LOCUS       DACBZN010000001        93451 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.001, whole genome shotgun sequence.
ACCESSION   DACBZN010000001 DACBZN010000000
VERSION     DACBZN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.001"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            83..970
                     /locus_tag="IVD64_000001"
     CDS             83..970
                     /locus_tag="IVD64_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6147288.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IVD64_000002"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IVD64_000002"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP6147289.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IVD64_000003"
     CDS             2272..2718
                     /locus_tag="IVD64_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6147290.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IVD64_000004"
     CDS             2991..4343
                     /locus_tag="IVD64_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP6147291.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IVD64_000005"
     CDS             4514..4801
                     /locus_tag="IVD64_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147292.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IVD64_000006"
     CDS             5027..5470
                     /locus_tag="IVD64_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6147293.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IVD64_000007"
     CDS             complement(5476..6660)
                     /locus_tag="IVD64_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP6147294.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IVD64_000008"
     CDS             6928..7626
                     /locus_tag="IVD64_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP6147295.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IVD64_000009"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IVD64_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP6147296.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IVD64_000010"
     CDS             complement(8436..8969)
                     /locus_tag="IVD64_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147297.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IVD64_000011"
     CDS             complement(9266..11647)
                     /locus_tag="IVD64_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6147298.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IVD64_000012"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IVD64_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP6147299.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IVD64_000013"
     CDS             12348..12887
                     /locus_tag="IVD64_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP6147300.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IVD64_000014"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IVD64_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP6147301.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IVD64_000015"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVD64_000015"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IVD64_000016"
     CDS             13994..15160
                     /locus_tag="IVD64_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP6147302.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IVD64_000017"
     CDS             15206..16702
                     /locus_tag="IVD64_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP6147303.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IVD64_000018"
     CDS             16783..17466
                     /locus_tag="IVD64_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6147304.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IVD64_000019"
     CDS             18111..19301
                     /locus_tag="IVD64_000019"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP6147305.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IVD64_000020"
     CDS             19403..20356
                     /locus_tag="IVD64_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP6147306.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IVD64_000021"
     CDS             20425..21774
                     /locus_tag="IVD64_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6147307.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IVD64_000022"
     CDS             complement(21957..22280)
                     /locus_tag="IVD64_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147308.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IVD64_000023"
     CDS             22409..23572
                     /locus_tag="IVD64_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147309.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IVD64_000024"
     CDS             23642..24991
                     /locus_tag="IVD64_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6147310.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IVD64_000025"
     CDS             complement(25036..25467)
                     /locus_tag="IVD64_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6147311.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IVD64_000026"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IVD64_000026"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP6147312.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IVD64_000027"
     CDS             28697..29293
                     /locus_tag="IVD64_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6147313.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IVD64_000028"
     CDS             29415..30563
                     /locus_tag="IVD64_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6147314.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IVD64_000029"
     CDS             complement(30601..31014)
                     /locus_tag="IVD64_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP6147315.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IVD64_000030"
     CDS             31093..31524
                     /locus_tag="IVD64_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP6147316.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IVD64_000031"
     CDS             complement(31623..31856)
                     /locus_tag="IVD64_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP6147317.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHQ"
     gene            32039..33709
                     /locus_tag="IVD64_000032"
     CDS             32039..33709
                     /locus_tag="IVD64_000032"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP6147318.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IVD64_000033"
     CDS             33856..34242
                     /locus_tag="IVD64_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147319.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IVD64_000034"
     CDS             34229..34927
                     /locus_tag="IVD64_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6147320.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IVD64_000035"
     CDS             34932..35396
                     /locus_tag="IVD64_000035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP6147321.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IVD64_000036"
     CDS             35393..36304
                     /locus_tag="IVD64_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6147322.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IVD64_000037"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IVD64_000037"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP6147323.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IVD64_000038"
     CDS             37092..38375
                     /locus_tag="IVD64_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP6147324.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IVD64_000039"
     CDS             38388..39314
                     /locus_tag="IVD64_000039"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP6147325.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IVD64_000040"
     CDS             39333..40613
                     /locus_tag="IVD64_000040"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP6147326.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IVD64_000041"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IVD64_000041"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP6147327.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IVD64_000042"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IVD64_000042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP6147328.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IVD64_000043"
     CDS             44938..45714
                     /locus_tag="IVD64_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP6147329.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IVD64_000044"
     CDS             45711..46643
                     /locus_tag="IVD64_000044"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP6147330.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IVD64_000045"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IVD64_000045"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP6147331.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IVD64_000046"
     CDS             47363..47992
                     /locus_tag="IVD64_000046"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP6147332.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IVD64_000047"
     CDS             47980..48294
                     /locus_tag="IVD64_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147333.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IVD64_000048"
     CDS             48332..48973
                     /locus_tag="IVD64_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6147334.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IVD64_000049"
     CDS             49004..49708
                     /locus_tag="IVD64_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP6147335.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IVD64_000050"
     CDS             49705..50607
                     /locus_tag="IVD64_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6147336.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IVD64_000051"
     CDS             complement(50765..52471)
                     /locus_tag="IVD64_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP6147337.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IVD64_000052"
     CDS             52661..53284
                     /locus_tag="IVD64_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147338.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="IVD64_000053"
     CDS             53734..54783
                     /locus_tag="IVD64_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6147339.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="IVD64_000054"
     CDS             54780..55661
                     /locus_tag="IVD64_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147340.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="IVD64_000055"
     CDS             55664..56434
                     /locus_tag="IVD64_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6147341.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="IVD64_000056"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="IVD64_000056"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP6147342.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="IVD64_000057"
     CDS             57745..58908
                     /locus_tag="IVD64_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6147343.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="IVD64_000058"
     CDS             59025..59435
                     /locus_tag="IVD64_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6147344.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="IVD64_000059"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="IVD64_000059"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6147345.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61975..62292
                     /locus_tag="IVD64_000060"
     CDS             61975..62292
                     /locus_tag="IVD64_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147346.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62452..62688
                     /locus_tag="IVD64_000061"
     CDS             62452..62688
                     /locus_tag="IVD64_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6147347.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63083
                     /locus_tag="IVD64_000062"
     CDS             62685..63083
                     /locus_tag="IVD64_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP6147348.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63750..64694)
                     /locus_tag="IVD64_000063"
     CDS             complement(63750..64694)
                     /locus_tag="IVD64_000063"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6147349.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64990..65934)
                     /locus_tag="IVD64_000064"
     CDS             complement(64990..65934)
                     /locus_tag="IVD64_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6147350.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66155..67369
                     /locus_tag="IVD64_000065"
     CDS             66155..67369
                     /locus_tag="IVD64_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6147351.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67391..69406
                     /locus_tag="IVD64_000066"
     CDS             67391..69406
                     /locus_tag="IVD64_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP6147352.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69489..70862)
                     /locus_tag="IVD64_000067"
     CDS             complement(69489..70862)
                     /locus_tag="IVD64_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6147353.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70888..71172)
                     /locus_tag="IVD64_000068"
     CDS             complement(70888..71172)
                     /locus_tag="IVD64_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6147354.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71174..73189)
                     /locus_tag="IVD64_000069"
     CDS             complement(71174..73189)
                     /locus_tag="IVD64_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6147355.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73368..73673)
                     /locus_tag="IVD64_000070"
     CDS             complement(73368..73673)
                     /locus_tag="IVD64_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP6147356.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73762..74793)
                     /locus_tag="IVD64_000071"
     CDS             complement(73762..74793)
                     /locus_tag="IVD64_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6147357.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75000..75875
                     /locus_tag="IVD64_000072"
     CDS             75000..75875
                     /locus_tag="IVD64_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6147358.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76027..76869)
                     /locus_tag="IVD64_000073"
     CDS             complement(76027..76869)
                     /locus_tag="IVD64_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6147359.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76961..78838)
                     /locus_tag="IVD64_000074"
     CDS             complement(76961..78838)
                     /locus_tag="IVD64_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6147360.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78835..80256)
                     /locus_tag="IVD64_000075"
     CDS             complement(78835..80256)
                     /locus_tag="IVD64_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6147361.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80259..83648)
                     /locus_tag="IVD64_000076"
     CDS             complement(80259..83648)
                     /locus_tag="IVD64_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6147362.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83905..85296
                     /locus_tag="IVD64_000077"
     CDS             83905..85296
                     /locus_tag="IVD64_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6147363.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85369..86016)
                     /locus_tag="IVD64_000078"
     CDS             complement(85369..86016)
                     /locus_tag="IVD64_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6147364.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86024..86710)
                     /locus_tag="IVD64_000079"
     CDS             complement(86024..86710)
                     /locus_tag="IVD64_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147365.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86716..87788)
                     /locus_tag="IVD64_000080"
                     /pseudo
     CDS             complement(86716..87788)
                     /locus_tag="IVD64_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            88010..88684
                     /locus_tag="IVD64_000081"
     CDS             88010..88684
                     /locus_tag="IVD64_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6147366.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88689..90029
                     /locus_tag="IVD64_000082"
     CDS             88689..90029
                     /locus_tag="IVD64_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6147367.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90178..91164)
                     /locus_tag="IVD64_000083"
     CDS             complement(90178..91164)
                     /locus_tag="IVD64_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP6147368.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91888..92919
                     /locus_tag="IVD64_000084"
     CDS             91888..92919
                     /locus_tag="IVD64_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6147369.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93028..>93451
                     /locus_tag="IVD64_000085"
     CDS             93028..>93451
                     /locus_tag="IVD64_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6147370.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        29894 a        16573 c        18963 g        28021 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat ttcagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattgat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag caaccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaaccaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg atttgactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg tcactcgcca ggcgaaatga
    62761 tcggtgtaaa agatgcaaat gcattagata tggccgtcaa acagccttca caagcagtat
    62821 tcaatcaaga actctatcct gaaatctatg aaaaagcggc gattttagca attaatttag
    62881 ctaaaaaaca tccttttcat aatggcaata aacgaactgc tcttgttgcc atgttgttgt
    62941 ttcttcaatt gaataattgt ccagttgctt tttctcgtca agaagctgtt gattttatca
    63001 taatgattac cactagttca ttggatttcg attcactaaa gtccaagata accagttatt
    63061 tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta tttctcaatt agatcagcat
    63121 gtcattaagg aatatgctga tttttttatg tttaatcata caaaatagtt ttttcataaa
    63181 aatatggaaa atatggatta attttgaaat aattgattta aaaggaatac aaaagagtga
    63241 atagtaggaa atagtggttt agaactagtg ggctgatttt ttatatattt tagtttacat
    63301 aatatatatt atacaaagta gatttaaaaa atgataaacc tctctcgaac ggttttttat
    63361 caaaaatcgt catttttcca tcaaaatctg cttatgcaaa attataaaaa aattttgcag
    63421 tttttcttac tttttttgtc gtttaattga atgataaaaa ccagtttctt tattcagtta
    63481 atcaaatatg aacgaattag atatgaatca ttgtatttgc cgttgcggaa ggatttttta
    63541 attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga ttaaatttca
    63601 tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc tgtttatttg
    63661 cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt taagcagttt
    63721 taggattttt agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa
    63781 gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact
    63841 tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg
    63901 tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct
    63961 tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg
    64021 atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata
    64081 cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat
    64141 gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca
    64201 acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta
    64261 ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct
    64321 actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg
    64381 atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt
    64441 tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct
    64501 gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc
    64561 ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg
    64621 atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga
    64681 cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag
    64741 tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt
    64801 tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat
    64861 cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa
    64921 cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag
    64981 attttcttct taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa
    65041 taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga
    65101 aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc
    65161 ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc
    65221 aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa
    65281 tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa
    65341 taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg
    65401 actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt
    65461 ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat
    65521 caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa
    65581 ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct
    65641 ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc
    65701 attaataatt tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt
    65761 atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa
    65821 tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt
    65881 gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa
    65941 tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta
    66001 ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata aagactttct
    66061 atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat
    66121 atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa
    66181 attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat
    66241 tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac
    66301 tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat
    66361 cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc
    66421 cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt
    66481 tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg
    66541 agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt
    66601 tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag
    66661 cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca
    66721 atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta
    66781 tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac
    66841 agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat
    66901 gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg
    66961 ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt
    67021 gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt
    67081 cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa
    67141 tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct
    67201 ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat
    67261 cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac
    67321 agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag
    67381 gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt
    67441 tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc
    67501 agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct
    67561 tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg
    67621 acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat
    67681 ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc
    67741 agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct
    67801 ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc
    67861 agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg
    67921 ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga
    67981 gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag
    68041 aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg
    68101 aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc
    68161 aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg
    68221 tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc
    68281 gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac
    68341 gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta
    68401 ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt
    68461 tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa
    68521 tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat
    68581 ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat
    68641 gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca
    68701 tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga
    68761 agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc
    68821 gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa
    68881 gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga
    68941 acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta
    69001 ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca
    69061 aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc
    69121 atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa
    69181 gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg
    69241 tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat
    69301 tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc
    69361 aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct
    69421 tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg
    69481 aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag
    69541 ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg
    69601 tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta
    69661 gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc
    69721 aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg
    69781 aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta
    69841 gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag
    69901 ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta
    69961 gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc
    70021 attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt
    70081 gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct
    70141 aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct
    70201 cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca
    70261 aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca
    70321 acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt
    70381 aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc
    70441 gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga
    70501 aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc
    70561 atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg
    70621 ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt
    70681 gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata
    70741 gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc
    70801 aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc
    70861 attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc
    70921 ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga
    70981 agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac
    71041 acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag
    71101 tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc
    71161 aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg
    71221 atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca
    71281 aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat
    71341 gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt
    71401 cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa
    71461 acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat
    71521 tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat
    71581 ccgataattg gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct
    71641 tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta
    71701 cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca
    71761 ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca
    71821 agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag
    71881 gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg
    71941 aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca
    72001 atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt
    72061 ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt
    72121 cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt
    72181 agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat
    72241 aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa
    72301 acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt
    72361 ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa
    72421 gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt
    72481 gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca
    72541 tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc
    72601 gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt
    72661 tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa
    72721 gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc
    72781 ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt
    72841 catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt
    72901 gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc
    72961 ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc
    73021 agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag
    73081 gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta
    73141 gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca
    73201 cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt
    73261 acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa
    73321 agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca
    73381 tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg
    73441 atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa
    73501 ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct
    73561 tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta
    73621 ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt
    73681 cctcctagta gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca
    73741 aacccgattc ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc
    73801 ttcttccaaa attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc
    73861 attaactaat gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat
    73921 tactttttct tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc
    73981 gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc
    74041 ttgtcgatct tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg
    74101 acgttctttg atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa
    74161 atccagatcg aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct
    74221 gatatcatct ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc
    74281 atataaatag ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa
    74341 ataatcataa tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt
    74401 ttggactgtc aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc
    74461 tttttctttt gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt
    74521 ctctactagt acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg
    74581 actaggtgta gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg
    74641 atcagtatat tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt
    74701 tttgatattg attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact
    74761 ctttccattg ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt
    74821 tcagtatatc ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc
    74881 agaaaaacac ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat
    74941 caaaaagata gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca
    75001 tgcttattga agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg
    75061 tcgattacat ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa
    75121 aagctacgta tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg
    75181 gatttaaaga attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca
    75241 atcaaacgat tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa
    75301 agaaaatggc ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg
    75361 aagatttaga tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa
    75421 aaggtgattc tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt
    75481 ttttgatcat ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg
    75541 aggattgcgc catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga
    75601 cctatttgaa gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa
    75661 tggccaaaca ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag
    75721 tagcgacttt ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga
    75781 tatattctca aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc
    75841 aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag
    75901 aaagatagtg tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt
    75961 tttcaaacct cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag
    76021 gaatagctaa cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag
    76081 gaaaatcagt atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac
    76141 tttctgtact tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag
    76201 taagcgatga ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc
    76261 tttttcgatt ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg
    76321 ataagcgagt gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg
    76381 taagtctgtg aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt
    76441 attttctccc cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag
    76501 cacagtattc gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat
    76561 accgatcgta tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt
    76621 aaagggatct gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat
    76681 gttttcttgg attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg
    76741 agagatgatc tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag
    76801 tatccctatt cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt
    76861 tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt
    76921 gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc
    76981 gtctagcaaa gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat
    77041 agattccctt accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag
    77101 gagaaatcaa ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt
    77161 ttgttaatgg aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt
    77221 ttagacctgt cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt
    77281 tcgccgatac ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt
    77341 cactatcttg atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt
    77401 caccatctac tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt
    77461 gatcatttgt atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg
    77521 ctttttcgtt caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag
    77581 gaatataatt cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat
    77641 ttactgggtc gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat
    77701 cattatcaaa tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt
    77761 tttgcgttcc ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa
    77821 ttttttcacc gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag
    77881 acgtatcatt tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag
    77941 gcgtgattgt ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat
    78001 tatattttcc aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg
    78061 caaagtcatt gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt
    78121 gatcagttgt ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg
    78181 gtgttccttc ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag
    78241 taccgatttt tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat
    78301 ggatagtatc tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct
    78361 catatacagg gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt
    78421 ctttgatcgt gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat
    78481 ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg
    78541 cttgttttgc tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga
    78601 cgttataaac tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt
    78661 ctttcccgct gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga
    78721 tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta
    78781 atggtaaaat aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt
    78841 tttcactcct gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc
    78901 aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt
    78961 ttctttttta ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt
    79021 tgaaatggta aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc
    79081 ttttaatcga taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga
    79141 aatcaggtga agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg
    79201 gaggtaattt cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg
    79261 tttatttgtc tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt
    79321 actgacacca aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc
    79381 tgtactgctt agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa
    79441 cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc
    79501 tgtcagcttt tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc
    79561 atccgcacta tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt
    79621 atgcaaggaa taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac
    79681 cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt
    79741 tgaaagtttt tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt
    79801 ttctttgacg acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc
    79861 ttgatcttta gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt
    79921 tgttgcttct gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc
    79981 ttcttctacc gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac
    80041 ttggaatgtg acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc
    80101 atttggatga tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg
    80161 aaccatttct gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa
    80221 gaagagacac atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt
    80281 ttcaaaaaaa gataccaaat agaaaacgca caaccaatca tccctactag atagattcct
    80341 aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa
    80401 gaaatctggt tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact
    80461 tgaattgacc catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct
    80521 tctggtgtaa aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag
    80581 gtatctgttt cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg
    80641 aattttgctc cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac
    80701 gcttttagtc gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt
    80761 cccgtgtaaa tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta
    80821 tcatatccgg caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca
    80881 aaactggcaa gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt
    80941 gcttcttttt tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct
    81001 tgcatcgcat acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac
    81061 tggatcgtat catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa
    81121 gcgatcttga tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt
    81181 tctgtcagcg tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt
    81241 ccgtcgccat gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg
    81301 ccaataagat ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg
    81361 aattgtttag tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca
    81421 ggtaattctt tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt
    81481 gataatatct cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt
    81541 tcccacgtat ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa
    81601 gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca
    81661 gctagattgt tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct
    81721 ttagcagatg gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt
    81781 gtcctgccgt tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt
    81841 ctcacttggt aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg
    81901 atggtatttc cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc
    81961 ttgactgaaa gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt
    82021 ccattgctta cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct
    82081 gttgcatgat cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt
    82141 cgcatacgag actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg
    82201 atgccaagac catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt
    82261 gcgataaacg tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt
    82321 cttgagatta gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa
    82381 taattgctgc ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca
    82441 tccgtcagca aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca
    82501 tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt
    82561 acttgatttt tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat
    82621 ccttcgcttg agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct
    82681 aaagtatcga caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc
    82741 atacttcctg accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca
    82801 tttccacgag cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac
    82861 tttcgtatcg catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc
    82921 cgatcattag gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta
    82981 ttgccttgat gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa
    83041 gtgccctgat catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca
    83101 gctggcaagg ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat
    83161 gattcttctt ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct
    83221 gtctcatcaa taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg
    83281 taaagtgctg gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt
    83341 tccaacgtaa ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc
    83401 acatcactta agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg
    83461 ttattcgcat ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca
    83521 gctgaaattg tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc
    83581 acaaagtttg ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg
    83641 gtcgtcattt ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat
    83701 taaaataaac aggcgttaca taattttatt tattttaata tgataattat tatacgaagg
    83761 gcgctaaagg gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc
    83821 ttcgattaat gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg
    83881 ttgttggaac gaaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac
    83941 aagtggatga ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta
    84001 aggtatatcg aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa
    84061 aaattggggt atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa
    84121 atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa
    84181 acgattgagt attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg
    84241 aaacaatgca atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt
    84301 cagttaacta gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta
    84361 agttactacg gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt
    84421 atggtcaaga ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct
    84481 ttctttatta tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa
    84541 tggaaagctg gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc
    84601 ttgaaaaatc tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt
    84661 gtcaatacga ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata
    84721 cgaaaaaata tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa
    84781 tatgttgttc aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg
    84841 actcatttga cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt
    84901 ctatatgctg gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg
    84961 atttattatt tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat
    85021 tatacttctg ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat
    85081 attgaagaat atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct
    85141 attactgaag atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat
    85201 caacccattt tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca
    85261 aatgaagaag aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata
    85321 aaccaccctg ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc
    85381 ttcagcaatc caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt
    85441 atttgcaatg agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact
    85501 aactgcttct tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt
    85561 ttcgttcatt ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg
    85621 ctgggttaac aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca
    85681 ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa
    85741 gatatcttga tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa
    85801 atcttgaaaa tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga
    85861 gactttgact tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct
    85921 ctgtttcttt gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt
    85981 atctctgatt atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct
    86041 cttcttctgt caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac
    86101 ggacatcatg agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta
    86161 atttgacgac ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga
    86221 tcaagcttgg ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag
    86281 aaaggtcttt aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat
    86341 cgatttttgt tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct
    86401 ttaccgttaa aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc
    86461 gtaaatctgc tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt
    86521 ctccggaagt cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac
    86581 cagaaggacc aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca
    86641 aagctgagac ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata
    86701 tttttttcat ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg
    86761 tatcgaaaac aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca
    86821 ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc
    86881 aaacaaaaga gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga
    86941 tttagcgatg aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt
    87001 ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa
    87061 gtatatcatc gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa
    87121 tgtccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    87181 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    87241 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    87301 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    87361 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    87421 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    87481 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    87541 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    87601 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    87661 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    87721 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    87781 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    87841 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    87901 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    87961 ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact
    88021 attagtagta gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat
    88081 cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt
    88141 ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc
    88201 aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt
    88261 ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta
    88321 tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga
    88381 tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta
    88441 taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca
    88501 aaataaggga atcattttga caaaagagca gatatttgac cggatctggg gatttgattc
    88561 tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag
    88621 tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt
    88681 gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg
    88741 tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc
    88801 aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag
    88861 tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg
    88921 aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca
    88981 ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc
    89041 gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata
    89101 ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag
    89161 cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca
    89221 agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc
    89281 tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt
    89341 ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga
    89401 ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat
    89461 ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt
    89521 tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata
    89581 cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa
    89641 aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc
    89701 atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga
    89761 tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc
    89821 cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat
    89881 ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg
    89941 aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag
    90001 tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa
    90061 atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga
    90121 aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta
    90181 cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc
    90241 tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac
    90301 ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt
    90361 aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt
    90421 gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac
    90481 cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt
    90541 ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt
    90601 cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt
    90661 catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc
    90721 tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg
    90781 tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa
    90841 ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt
    90901 ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc
    90961 ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa
    91021 tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc
    91081 ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat
    91141 gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta
    91201 tattgttact tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg
    91261 gaatttgatt aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat
    91321 tctaataaaa tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca
    91381 ctcgttgtga tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag
    91441 gattaattct caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag
    91501 taccaactat ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat
    91561 tattgaataa atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata
    91621 aaagaaagag cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac
    91681 cacatagcta aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa
    91741 ataactaaaa atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg
    91801 cttcctaaga tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa
    91861 aatgaacact attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa
    91921 aagttaaagc aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat
    91981 cgttcttccc tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact
    92041 gattatttga gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa
    92101 aaacaagatt atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact
    92161 acagcgaacc aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag
    92221 caatattcat taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag
    92281 atttctcttt tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat
    92341 gcagaacttt tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc
    92401 agagaagcta gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt
    92461 ctgtattgcg gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg
    92521 accaagcaga ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca
    92581 aaagaagcga tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa
    92641 aattcttttg tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa
    92701 attttgagca aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat
    92761 cgttattatg cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat
    92821 cgatacctct catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat
    92881 cacgaattag cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt
    92941 tcacaataaa ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat
    93001 tacttaacaa aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac
    93061 ttatttacga attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat
    93121 gagccacttt cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa
    93181 agtgtgttga aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt
    93241 attatttata agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt
    93301 ggtgcagtgc ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc
    93361 gatcgtttag aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa
    93421 atttcaaatg gaacaaaatc aaaacagatt c
//
LOCUS       DACBZN010000002        92615 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.002, whole genome shotgun sequence.
ACCESSION   DACBZN010000002 DACBZN010000000
VERSION     DACBZN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.002"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            160..840
                     /locus_tag="IVD64_000086"
     CDS             160..840
                     /locus_tag="IVD64_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6147371.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IVD64_000087"
     CDS             930..1964
                     /locus_tag="IVD64_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6147372.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IVD64_000088"
     CDS             1978..2313
                     /locus_tag="IVD64_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147373.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IVD64_000089"
     CDS             2297..4777
                     /locus_tag="IVD64_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147374.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IVD64_000090"
     CDS             4833..6392
                     /locus_tag="IVD64_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6147375.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IVD64_000091"
     CDS             6389..7753
                     /locus_tag="IVD64_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6147376.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IVD64_000092"
     CDS             7924..8994
                     /locus_tag="IVD64_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147377.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IVD64_000093"
     CDS             9009..9677
                     /locus_tag="IVD64_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147378.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IVD64_000094"
     CDS             9697..10275
                     /locus_tag="IVD64_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6147379.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IVD64_000095"
     CDS             10676..11587
                     /locus_tag="IVD64_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6147380.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IVD64_000096"
     CDS             complement(11965..12882)
                     /locus_tag="IVD64_000096"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP6147381.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IVD64_000097"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IVD64_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP6147382.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IVD64_000098"
     CDS             13579..14127
                     /locus_tag="IVD64_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP6147383.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IVD64_000099"
     CDS             14242..16602
                     /locus_tag="IVD64_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6147384.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IVD64_000100"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IVD64_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP6147385.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IVD64_000101"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IVD64_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP6147386.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IVD64_000102"
     CDS             19108..20007
                     /locus_tag="IVD64_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147387.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IVD64_000103"
     CDS             20000..21262
                     /locus_tag="IVD64_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147388.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IVD64_000104"
     CDS             21423..23321
                     /locus_tag="IVD64_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP6147389.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IVD64_000105"
     CDS             complement(23651..24370)
                     /locus_tag="IVD64_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP6147390.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24706..25038
                     /locus_tag="IVD64_000106"
     CDS             24706..25038
                     /locus_tag="IVD64_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147391.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25025..27028
                     /locus_tag="IVD64_000107"
     CDS             25025..27028
                     /locus_tag="IVD64_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP6147392.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27047..27517
                     /locus_tag="IVD64_000108"
     CDS             27047..27517
                     /locus_tag="IVD64_000108"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP6147393.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27538..28122
                     /locus_tag="IVD64_000109"
     CDS             27538..28122
                     /locus_tag="IVD64_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP6147394.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28137..29123
                     /locus_tag="IVD64_000110"
     CDS             28137..29123
                     /locus_tag="IVD64_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP6147395.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29113..29424
                     /locus_tag="IVD64_000111"
     CDS             29113..29424
                     /locus_tag="IVD64_000111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP6147396.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29446..31227
                     /locus_tag="IVD64_000112"
     CDS             29446..31227
                     /locus_tag="IVD64_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP6147397.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31220..32596
                     /locus_tag="IVD64_000113"
     CDS             31220..32596
                     /locus_tag="IVD64_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP6147398.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32603..33238
                     /locus_tag="IVD64_000114"
     CDS             32603..33238
                     /locus_tag="IVD64_000114"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP6147399.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33268..34623
                     /locus_tag="IVD64_000115"
     CDS             33268..34623
                     /locus_tag="IVD64_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP6147400.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34815..36209)
                     /locus_tag="IVD64_000116"
     CDS             complement(34815..36209)
                     /locus_tag="IVD64_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6147401.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36301..37272
                     /locus_tag="IVD64_000117"
     CDS             36301..37272
                     /locus_tag="IVD64_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP6147402.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37269..38051
                     /locus_tag="IVD64_000118"
     CDS             37269..38051
                     /locus_tag="IVD64_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP6147403.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38220..38591)
                     /locus_tag="IVD64_000119"
     CDS             complement(38220..38591)
                     /locus_tag="IVD64_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6147404.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38725..39168
                     /locus_tag="IVD64_000120"
     CDS             38725..39168
                     /locus_tag="IVD64_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6147405.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39211..40020)
                     /gene="yidA"
                     /locus_tag="IVD64_000121"
     CDS             complement(39211..40020)
                     /gene="yidA"
                     /locus_tag="IVD64_000121"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP6147406.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40039..41406)
                     /locus_tag="IVD64_000122"
     CDS             complement(40039..41406)
                     /locus_tag="IVD64_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6147407.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEYIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41390..42220)
                     /locus_tag="IVD64_000123"
     CDS             complement(41390..42220)
                     /locus_tag="IVD64_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP6147408.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42383..42820
                     /locus_tag="IVD64_000124"
     CDS             42383..42820
                     /locus_tag="IVD64_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP6147409.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42896..43516
                     /locus_tag="IVD64_000125"
     CDS             42896..43516
                     /locus_tag="IVD64_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP6147410.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43705..44211
                     /locus_tag="IVD64_000126"
     CDS             43705..44211
                     /locus_tag="IVD64_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP6147411.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44228..45127
                     /locus_tag="IVD64_000127"
     CDS             44228..45127
                     /locus_tag="IVD64_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP6147412.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45214..46098
                     /locus_tag="IVD64_000128"
     CDS             45214..46098
                     /locus_tag="IVD64_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6147413.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46290..46361)
                     /locus_tag="IVD64_000129"
     tRNA            complement(46290..46361)
                     /locus_tag="IVD64_000129"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46326..46328),aa:Arg,seq:ccg)
     gene            46421..47755
                     /gene="rpoN"
                     /locus_tag="IVD64_000130"
     CDS             46421..47755
                     /gene="rpoN"
                     /locus_tag="IVD64_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP6147414.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47958..48995
                     /locus_tag="IVD64_000131"
     CDS             47958..48995
                     /locus_tag="IVD64_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP6147415.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            49036..50037
                     /gene="gap"
                     /locus_tag="IVD64_000132"
     CDS             49036..50037
                     /gene="gap"
                     /locus_tag="IVD64_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6147416.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50147..51337
                     /locus_tag="IVD64_000133"
     CDS             50147..51337
                     /locus_tag="IVD64_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP6147417.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51441..52196
                     /locus_tag="IVD64_000134"
     CDS             51441..52196
                     /locus_tag="IVD64_000134"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP6147418.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52312..53610
                     /gene="eno"
                     /locus_tag="IVD64_000135"
     CDS             52312..53610
                     /gene="eno"
                     /locus_tag="IVD64_000135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP6147419.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            54010..55149
                     /locus_tag="IVD64_000136"
     CDS             54010..55149
                     /locus_tag="IVD64_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP6147420.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEELTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(55216..55791)
                     /locus_tag="IVD64_000137"
     CDS             complement(55216..55791)
                     /locus_tag="IVD64_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6147421.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(55796..56770)
                     /locus_tag="IVD64_000138"
     CDS             complement(55796..56770)
                     /locus_tag="IVD64_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP6147422.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(56794..56838)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            57053..58021
                     /locus_tag="IVD64_000139"
     CDS             57053..58021
                     /locus_tag="IVD64_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6147423.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            58134..58412
                     /locus_tag="IVD64_000140"
     CDS             58134..58412
                     /locus_tag="IVD64_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6147424.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCTYAKKEFEKKPEYQDVLRQS"
     gene            58530..58766
                     /gene="secG"
                     /locus_tag="IVD64_000141"
     CDS             58530..58766
                     /gene="secG"
                     /locus_tag="IVD64_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP6147425.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            58878..59624
                     /locus_tag="IVD64_000142"
     CDS             58878..59624
                     /locus_tag="IVD64_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6147426.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            59627..61984
                     /gene="rnr"
                     /locus_tag="IVD64_000143"
     CDS             59627..61984
                     /gene="rnr"
                     /locus_tag="IVD64_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP6147427.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            62006..62470
                     /gene="smpB"
                     /locus_tag="IVD64_000144"
     CDS             62006..62470
                     /gene="smpB"
                     /locus_tag="IVD64_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP6147428.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            62772..63491
                     /gene="atpB"
                     /locus_tag="IVD64_000145"
     CDS             62772..63491
                     /gene="atpB"
                     /locus_tag="IVD64_000145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP6147429.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            63544..63759
                     /gene="atpE"
                     /locus_tag="IVD64_000146"
     CDS             63544..63759
                     /gene="atpE"
                     /locus_tag="IVD64_000146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP6147430.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            63850..64374
                     /gene="atpF"
                     /locus_tag="IVD64_000147"
     CDS             63850..64374
                     /gene="atpF"
                     /locus_tag="IVD64_000147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP6147431.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            64361..64903
                     /locus_tag="IVD64_000148"
     CDS             64361..64903
                     /locus_tag="IVD64_000148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP6147432.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            64926..66482
                     /locus_tag="IVD64_000149"
     CDS             64926..66482
                     /locus_tag="IVD64_000149"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP6147433.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            66496..67398
                     /locus_tag="IVD64_000150"
     CDS             66496..67398
                     /locus_tag="IVD64_000150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP6147434.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            67421..68827
                     /gene="atpD"
                     /locus_tag="IVD64_000151"
     CDS             67421..68827
                     /gene="atpD"
                     /locus_tag="IVD64_000151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP6147435.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            68841..69263
                     /locus_tag="IVD64_000152"
     CDS             68841..69263
                     /locus_tag="IVD64_000152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP6147436.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            69732..69962
                     /locus_tag="IVD64_000153"
     CDS             69732..69962
                     /locus_tag="IVD64_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP6147437.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            70059..71360
                     /gene="murA"
                     /locus_tag="IVD64_000154"
     CDS             70059..71360
                     /gene="murA"
                     /locus_tag="IVD64_000154"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6147438.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            71373..71549
                     /locus_tag="IVD64_000155"
     CDS             71373..71549
                     /locus_tag="IVD64_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6147439.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            71684..71980
                     /gene="yidD"
                     /locus_tag="IVD64_000156"
     CDS             71684..71980
                     /gene="yidD"
                     /locus_tag="IVD64_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP6147440.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            72164..72631
                     /locus_tag="IVD64_000157"
     CDS             72164..72631
                     /locus_tag="IVD64_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6147441.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            72716..73600
                     /locus_tag="IVD64_000158"
     CDS             72716..73600
                     /locus_tag="IVD64_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6147442.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            73745..73951
                     /locus_tag="IVD64_000159"
     CDS             73745..73951
                     /locus_tag="IVD64_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP6147443.1"
                     /translation="MRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGPKEYEIFFGE
                     EDFGKEKSLENAIETVIRHWNLHE"
     gene            74039..74119
                     /locus_tag="IVD64_000160"
     tRNA            74039..74119
                     /locus_tag="IVD64_000160"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74073..74075,aa:Tyr,seq:gta)
     gene            74126..74197
                     /locus_tag="IVD64_000161"
     tRNA            74126..74197
                     /locus_tag="IVD64_000161"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74158..74160,aa:Gln,seq:ttg)
     gene            74712..75419
                     /locus_tag="IVD64_000162"
     CDS             74712..75419
                     /locus_tag="IVD64_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6147444.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(75479..76123)
                     /locus_tag="IVD64_000163"
     CDS             complement(75479..76123)
                     /locus_tag="IVD64_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP6147445.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            76250..76537
                     /locus_tag="IVD64_000164"
     CDS             76250..76537
                     /locus_tag="IVD64_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147446.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            76645..76965
                     /locus_tag="IVD64_000165"
     CDS             76645..76965
                     /locus_tag="IVD64_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6147447.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            77046..78359
                     /locus_tag="IVD64_000166"
     CDS             77046..78359
                     /locus_tag="IVD64_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6147448.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            78356..79075
                     /locus_tag="IVD64_000167"
     CDS             78356..79075
                     /locus_tag="IVD64_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP6147449.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            79203..79754
                     /gene="raiA"
                     /locus_tag="IVD64_000168"
     CDS             79203..79754
                     /gene="raiA"
                     /locus_tag="IVD64_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP6147450.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            79976..82510
                     /gene="secA"
                     /locus_tag="IVD64_000169"
     CDS             79976..82510
                     /gene="secA"
                     /locus_tag="IVD64_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP6147451.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            82636..83737
                     /gene="prfB"
                     /locus_tag="IVD64_000170"
     CDS             join(82636..82698,82700..83737)
                     /gene="prfB"
                     /locus_tag="IVD64_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP6147452.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            83842..84528
                     /gene="ftsE"
                     /locus_tag="IVD64_000171"
     CDS             83842..84528
                     /gene="ftsE"
                     /locus_tag="IVD64_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP6147453.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            84512..85405
                     /locus_tag="IVD64_000172"
     CDS             84512..85405
                     /locus_tag="IVD64_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147454.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            85557..87005
                     /gene="cls"
                     /locus_tag="IVD64_000173"
     CDS             85557..87005
                     /gene="cls"
                     /locus_tag="IVD64_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6147455.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            87342..88199
                     /locus_tag="IVD64_000174"
     CDS             87342..88199
                     /locus_tag="IVD64_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP6147456.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            88314..89234
                     /gene="pstC"
                     /locus_tag="IVD64_000175"
     CDS             88314..89234
                     /gene="pstC"
                     /locus_tag="IVD64_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP6147457.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            89234..90118
                     /gene="pstA"
                     /locus_tag="IVD64_000176"
     CDS             89234..90118
                     /gene="pstA"
                     /locus_tag="IVD64_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP6147458.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            90133..90939
                     /gene="pstB"
                     /locus_tag="IVD64_000177"
     CDS             90133..90939
                     /gene="pstB"
                     /locus_tag="IVD64_000177"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6147459.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            90955..91713
                     /locus_tag="IVD64_000178"
     CDS             90955..91713
                     /locus_tag="IVD64_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6147460.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            91726..92403
                     /gene="phoU"
                     /locus_tag="IVD64_000179"
     CDS             91726..92403
                     /gene="phoU"
                     /locus_tag="IVD64_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP6147461.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT        30685 a        16128 c        19804 g        25998 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaatta
    24421 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    24481 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    24541 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    24601 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    24661 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    24721 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    24781 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    24841 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    24901 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    24961 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    25021 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    25081 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    25141 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    25201 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    25261 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    25321 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    25381 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    25441 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    25501 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    25561 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    25621 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    25681 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    25741 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    25801 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    25861 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    25921 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    25981 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    26041 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    26101 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    26161 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    26221 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    26281 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    26341 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    26401 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    26461 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    26521 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    26581 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    26641 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    26701 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    26761 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    26821 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    26881 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    26941 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    27001 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    27061 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    27121 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    27181 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca
    27241 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    27301 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    27361 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    27421 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    27481 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    27541 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    27601 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    27661 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    27721 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    27781 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    27841 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    27901 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    27961 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    28021 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    28081 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    28141 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    28201 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    28261 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    28321 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    28381 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    28441 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    28501 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    28561 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    28621 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    28681 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    28741 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    28801 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    28861 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    28921 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    28981 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    29041 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    29101 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    29161 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    29221 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    29281 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    29341 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    29401 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    29461 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    29521 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    29581 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    29641 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    29701 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    29761 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    29821 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    29881 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    29941 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    30001 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    30061 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    30121 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    30181 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    30241 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    30301 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    30361 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    30421 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    30481 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    30541 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    30601 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    30661 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    30721 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    30781 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    30841 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    30901 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    30961 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    31021 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    31081 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    31141 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    31201 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    31261 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    31321 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    31381 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    31441 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    31501 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    31561 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    31621 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    31681 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    31741 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    31801 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    31861 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    31921 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    31981 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    32041 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    32101 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    32161 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    32221 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    32281 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    32341 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    32401 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    32461 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    32521 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    32581 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    32641 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    32701 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    32761 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    32821 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    32881 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    32941 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    33001 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    33061 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    33121 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    33181 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    33241 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    33301 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    33361 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    33421 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    33481 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    33541 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    33601 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    33661 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    33721 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    33781 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    33841 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    33901 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    33961 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    34021 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    34081 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    34141 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    34201 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    34261 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    34321 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    34381 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    34441 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    34501 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    34561 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    34621 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    34681 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    34741 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    34801 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    34861 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    34921 gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    34981 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    35041 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    35101 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    35161 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    35221 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    35281 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    35341 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    35401 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    35461 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    35521 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    35581 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    35641 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    35701 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    35761 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    35821 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    35881 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    35941 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    36001 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    36061 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    36121 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    36181 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    36241 aattttacca ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct
    36301 atgaaaatta ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa
    36361 gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg
    36421 gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg
    36481 ggggagaaca gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat
    36541 catttacctt ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa
    36601 gcattgataa acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca
    36661 acagattact tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa
    36721 gcattgtcgc atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac
    36781 gatattttag aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa
    36841 ataacgttta taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt
    36901 acgttgacct ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat
    36961 atcaatgctg tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa
    37021 acttctcaaa tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt
    37081 atagccatgg cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta
    37141 gtttctgaga aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt
    37201 ggtctagcta ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg
    37261 gctttaaaat gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag
    37321 gaaatccagc agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa
    37381 ttgccattga aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg
    37441 agcttagatg gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa
    37501 cagcatttgt tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc
    37561 aaagcggtaa tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta
    37621 ttatgccgaa aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta
    37681 aagaagccta cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta
    37741 aattgcagcc agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga
    37801 cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca
    37861 atgaagatcc agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa
    37921 tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg
    37981 aattaaaaga agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct
    38041 cgtttatttg atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat
    38101 agctacaatg gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc
    38161 tcattttaat tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac
    38221 tatcctcgga tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc
    38281 gcagtgccag gtaatccttt taagtcagta atctcaagac cagcagcttt atgttgctcg
    38341 tgtaattttt ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca
    38401 tacgcaccat gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc
    38461 aaataaacaa gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca
    38521 aatgcctttt gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga
    38581 gccattttca tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa
    38641 ggaaatagtt gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata
    38701 aaactgttaa ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt
    38761 gctgattatc aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca
    38821 gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag
    38881 acgaaagaag aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt
    38941 tcttcttatc tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat
    39001 tataatccag gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa
    39061 aagaccaata aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt
    39121 agccaccgat gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt
    39181 agatcagctt tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc
    39241 tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac
    39301 ggcatttccc attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt
    39361 atctccgata gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac
    39421 tgccgcacct ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac
    39481 cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc
    39541 tggttcgtcg atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt
    39601 tcgatatttt agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc
    39661 tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg
    39721 tgccattact tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt
    39781 acctgatttc gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg
    39841 aaaaaggttc aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa
    39901 aacgatcgaa acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac
    39961 ttctcttttc tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat
    40021 aatttcctcc aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc
    40081 aaattcttga taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat
    40141 ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag
    40201 ctgactaacc tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt
    40261 gcgatgtctg ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac
    40321 agcagtgtaa taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac
    40381 taattttgaa tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa
    40441 acgtttagct aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt
    40501 gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa
    40561 tggcagaagt aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg
    40621 tgcgcgatga agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta
    40681 cacttgcaca tacatttgat atcggctaac gatataatct tccactgcat gcatgccact
    40741 catggaaaaa gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc
    40801 aaatgttcca tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc
    40861 tgcatcgatc tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat
    40921 cacactagct accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc
    40981 tgtttctgga gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatata
    41041 ttcaaatgta tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt
    41101 caccagccgt tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg
    41161 agaaaagata tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg
    41221 gctatgttct gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag
    41281 acgttggact tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata
    41341 gttatggact ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt
    41401 tgtcataatg tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc
    41461 attgctccag ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag
    41521 aatacagctt ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt
    41581 ggtcaagcag ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg
    41641 taccaaagtc ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccacgttttt
    41701 cctgattacc gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct
    41761 gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag
    41821 aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga
    41881 gcatttgctc gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca
    41941 aagaaatatt caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca
    42001 ctggagcgta acctgcatct tccagtgttt ggatcccgtc acttagaaaa ggagttcgaa
    42061 catccttcat acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct
    42121 ggtgttcttt agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt
    42181 tttgataaat gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata
    42241 aattcatatt ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt
    42301 ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat
    42361 gagaagtaaa ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt
    42421 gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca
    42481 aatggtgaca attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg
    42541 cgatgagatt cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc
    42601 aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg
    42661 aacaccatac ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa
    42721 agaccgtccc acagcaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa
    42781 aatcggcgaa tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat
    42841 tttttgtgtc tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga
    42901 aattagtgta tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc
    42961 gatcttagaa caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa
    43021 ctatcttggg aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt
    43081 gaatattgac ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta
    43141 tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac
    43201 tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat
    43261 cgattacaat gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct
    43321 gtttgatgac gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga
    43381 aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga
    43441 tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga
    43501 ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta
    43561 tcagaataag gaggtgtgac acgatttttg tcacaccttc ttttttgtat ataaaaatcc
    43621 caagtaaata cataaaaaat aaaaaaattg aaacttttag cgcaccttgt acgtcatatt
    43681 aagtgaggca gaaaggagtg gagaatgatt tttactggat ataagaaaaa acggcagatc
    43741 gaaaaatacc gtcacctgct gaatggactc atcgaaactg attatcaaaa aatgttccgt
    43801 atcacgatga attacgtaca tgataaagaa gaggccttag atgtgatgca agatagtttt
    43861 cataaagccc tgaactattt tgaaaaagaa aaagagatcg agcattttag tgcatggttt
    43921 tatcggatat tgatccgcac ggcattagat agttggcgca gacaaaaaag gacgcctgtg
    43981 gacttatttg aaattcaaga tatcccccaa cagttacctt tagaaacttc cgttgcagag
    44041 ctccatatga ttttagataa aatagacagt ccggaaaaag agattctgat cctctacttt
    44101 tttgaaggat ttcgtttgaa agaaattgcg actatcttgg atatgaatga aaacacagtt
    44161 aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg aaggagagaa
    44221 gagtgccatg tattcccaaa aagaaaattt aaaaaaacta aagaaaactt ataaagaaca
    44281 agcaattccc aaagggctta aagaggagat ccgccagcgt tttattatcg aagagcaatc
    44341 atttataaaa gcaaagcgga gaaagagacg tctccaaggg ttcggagtat ctttggctat
    44401 cgtaatgatt acaacaagta gccttctttt taatagtcaa gttcgaagct ttgcagaaga
    44461 tttacccatc ttaggatcag tcattgaatt gatcttaggc gaacgcttta ctgatcgatc
    44521 tgaaaaaatc gatattcagg taccgaagat caatacacaa aatgagaaag aaaataaaac
    44581 aattcatgga ttaaatcaaa aatactttcg cgaaggacaa gcagattttg aaaaagctga
    44641 aaaagaatat ggaaattttg aaacggatca ttaccaagtt ttgggagatt atcagaaaat
    44701 cgtagacgat aaccggtttc tcgtgattga aaggcagatc acccaaacag cagcagatag
    44761 ccatgttgaa aaaagatacg acacaatcga caagaaaaac agtgtccagc tctcacttcc
    44821 acttttattc aaagatgaca cctatctatc tgttttgaca aaagaagtca aacggcagat
    44881 ggccgaacaa gtcaaggaag atccatctaa gtactattgg actgagcaag atattcaaga
    44941 agggacgata gaaaaaccaa cacttgttac gcctacacga agtttttatt tgaacaaaga
    45001 gcatcaatta gttttgactt tttctcaata cgaaattgct cctggttata tgggtacacc
    45061 agaatttgtc attcctaagt ccgtgacaaa aacgattttg gcatcggaag attatttgga
    45121 tcattagacg agaagtagtt gcgactagca gtttcaattt tcaagtaagt acaagtaaat
    45181 aataagaaga gaaaaagaga aagaagagat atagtgaaaa gaaattattt agttatcagt
    45241 attattgctt tatccgtgct tttgtttttc gaaattactt cggtggcctc cttgatcacc
    45301 gaagtaaaag gacaaacaca ctcttcggag cattcgtcac aatctttcaa acaatcagaa
    45361 caatcaacca ttaaaagcca atcaactgaa acagctaacg tcaaagaagc cattcaagaa
    45421 tcagcggaaa aggtacaaag tcagtcatca caatcaacac aagacagtac aaaaacgagc
    45481 cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg agcgaccaca
    45541 acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta
    45601 ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc agggcacgag
    45661 attggcaatc acacatacga tcatcagaat ctggccactc tttctgccca gtcgatgacg
    45721 gaggaagtga cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac ccccactttt
    45781 gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc
    45841 atcgaatggt ctgttgattc agaagattgg aagacaagaa atcctgattt gattttgcag
    45901 aagatccaag caacagtata cgatggggca attattttat tccatgatat ctatcctgaa
    45961 acgattcgtg ccgttcctca agtgatcgat tatctgaaag agcaagggta tcgcatcacg
    46021 acagttgggg atttattagg gcatccaacg gctgtcgaga actattatgg cagaaacgat
    46081 catcggccag ttcagtaagt gagacaagta tctttaataa ataacacatt ttaataagta
    46141 gttgaacaca taactacctt acttgcaaag gcttaacaat atctatacag gtacaacaaa
    46201 aaactgtcca ccaattttct ttggcggaca gttttcactt aaaaataaag aagcaactat
    46261 tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca
    46321 agaaccggaa tcttacgtga tatccactac actaaggacg caagcactgg gataattgta
    46381 actaaacaga taaaaaatta caaggggatt ggacaaaaaa atgaaatttg agcaaaattt
    46441 ttcacaaaaa cagcagcaga cacagaaact agccatgacg caacagttgc aacagtccat
    46501 ccaagtttta caatataaca gtgaggagct gttggcattc gttgagaatc aagcaatgga
    46561 aaatccattg gtggaagttg ttgagccgga atggcagcca gattacataa aagcttcttc
    46621 atcttcttat gaaggagaag agacaaatta cctaaatcag attcctgata caaaagggtc
    46681 tttatttgat tcattgatcg aacaagtcca tttgaattac cgagatactt ttttgaggaa
    46741 aatcgttctt tatcttgtag aatacatcga tttgaacgga tttttgacga ttagtttaga
    46801 agaagccgga aaagagattg gagctacgcc tatccaaatg ctcgatgcgt tgactctgat
    46861 tcagcaatta gaaccagctg gagtaggtgc acgtagtttg caagaatgtc tcatgcttca
    46921 aacagaacga gatgattatg cgccggaatt agcttatatc gtgttggaag aatgttttga
    46981 ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg atctacatgc
    47041 tgttcagcag atattcgact atttacaaac gttgagtcca agccctggcc ggattttcga
    47101 ccgtagcagc gaattgtaca ttattcctga tgtccgggta ttagtggacg acgataaaaa
    47161 tgtacaagtt atctcaaacc gaaaaaatca gccaaatata cgctttcaag agagttactt
    47221 caaacaaatg tcacaacaag cagataaaga aactgaaaat tatttaaaag aacggaaaca
    47281 ggaattcgaa tggttgaaga aaacgatcct acaacgtggg gatacaattt tgcgggttgc
    47341 tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc
    47401 tcttacgtta aaggaagttg caactgagat agatgtgcat gaatccacgg tcagtcgtgc
    47461 agtcaacggg aaatatttgg aaacagactt cggtgtattt gaattaaaaa aatttttcac
    47521 tacacgaatt cctacgaata gtacagaaca gacagaagac ctttcagcag atacagcgaa
    47581 gaaaaagctg caggaattag tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa
    47641 attggttgaa ttgctgaaaa aagatgagat agcgatctca agacgaacag tagcaaaata
    47701 tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata actaatcagt
    47761 caaaagacta gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa acgagagcaa
    47821 aactgttaga cttacagtcg tgaggttgag atttcccttt attgatgtga aattcgaata
    47881 gttaatttct ttttttatcg tatatgggtc gttttaagtc tacattggac gtaaagtgtc
    47941 caactaagga gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt gcaccagata
    48001 ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa tatctattgg atgcagccga
    48061 ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga acgagtattg cgaacagaaa
    48121 cagatctttt gaaaaatctg aatttgatcg acacctcaaa aagcggtatg acgctgacaa
    48181 ctaagggcga agaagtttat caaagccttg aaaacttcat ggatcagctt ttaggtacgc
    48241 atcaaaccga acaacagttg gcagaatatt ttggtaacca acgctgtatc gttgtttcag
    48301 gaaacagcga agagcagacc aaagtggctg atgcattcgg cgaagcgctg agcgaagccc
    48361 tagaccgact acttccagaa ggcgagaata tcatcgctgt gatgggagga actactatgg
    48421 ctgtggtagc cgaacaattg tctaatctgg aaaataagaa acggcataac ctgtttgtcc
    48481 ctgcaagagg cgggattggt gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa
    48541 tggctgctaa agcaaacggt aaccaccgtg cactttatgt tccggaacaa ttaagtctgg
    48601 caacttacaa ttctttactc aatgagccat ctatccaaga agtcttaaac ttgattagtg
    48661 aagcgaattg tgttattcat agtattgggc gtgcattaca catggcagca cgtcgtaaga
    48721 tgactgaaaa agaattagtc atgctgaaac aagcaaatgc tgttgcggaa tcttttggct
    48781 actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc atagaattgc
    48841 aagatttgga aaaagtcccg atcatcatgg caatcgcggg cggaaaatca aaagccaaag
    48901 caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact gatgaagctg
    48961 ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa atattttttt gattttcata
    49021 aggaggaaat cttaaatgac agttaaagta ggtattaatg gttttggacg tattggacgt
    49081 ctagcattcc gtcgtatcca agatgtagat ggaatcgaag tagtagcaat caacgacttg
    49141 acagatgcta aaatgttggc acacttgtta aaatacgaca caactcaagg acgtttcaac
    49201 ggaacagttg aagttcatga aggttcattc aacgtaaacg gaaaagaagt taaagtatta
    49261 gctaaccgca acccagaaga attaccatgg ggcgaactag gcgtagatat cgttctagaa
    49321 tgtactggtt tcttcacttc taaatcagca gctgaaaaac atttaacagc tggtgctaaa
    49381 cgcgttgtta tctctgctcc tggcggaaac gatgttccaa caatcgttta taacacaaac
    49441 cacgaaacat taactggtaa agaaacagtt atctcaggtg cttcatgtac tactaactgt
    49501 ttagctccta tggctaaaac attgaacgac aaatttggtg ttgttgaagg attaatgaca
    49561 actatccacg cttacacagg tgaccaaatg actctagacg gacctcatcc taaaggtgac
    49621 ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta actcaacagg tgctgctaaa
    49681 gctatcggtt tagtaattcc tgaattgaac ggtaaattag acggagctgc tcaacgtgtt
    49741 cctgtaccaa caggttcatt aacagaatta gtaacagttc ttgaaaaaga agtaactgtt
    49801 gacgaaatca atgcagcaat gaaagaagct tcaaacgaat cttacggata caacacagac
    49861 gaaatcgttt cttcagatat cgttggtatg acttacggtt cattatttga tgctacacaa
    49921 actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac
    49981 gaaatgtcat acactgcaca attagtacgt actttagaat acttcgctaa cttataagat
    50041 agacaaacgt acaagtgtaa attatagaca tgtacattta aaagcgggga agcaatcgcg
    50101 cttcttccgc tttttttgat aataagttta ttcaggagga aataaaatgg ctaagaaaac
    50161 agtgaaagat atcgatttaa aagacaaaaa agttcttgtc cgtgttgact tcaacgttcc
    50221 tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa gcagcattgc caacaatcaa
    50281 ctacgttcta gaacaaggcg gaaaagcaat ccttttctct caccttggac gtgtaaaaac
    50341 agaagaagat aaagaaggaa aatctttagc tccagttgca aaacgtctag gcgaattatt
    50401 aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa caattagaag aagcaatccg
    50461 caacatgaat gacggcgacg ttgtcgtatt cgaaaataca cgatttgaag atattgacgg
    50521 taaaaaagaa agcaaaaatg atacagaact aggtaaatac tgggcttcat taggcgatgt
    50581 atttgttaac gatgctttcg gtacagctca ccgtgcacat gcttctaacg taggtatcgc
    50641 ttcaactggt atcccaacag ttgctggatt cttgatggaa aaagaaatca agttcatcgg
    50701 tgaagcagta gaagaaccaa aacgaccaat gattgcaatc cttggtggcg caaaagtttc
    50761 tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca gataaaatct tgatcggtgg
    50821 cggaatgact tacacattct acgaagcaaa aggaatcaag atcggtaact ctctagtaga
    50881 agctgataaa gtggaactag caaaagaatt aatcgaaaaa gcaggcgaca aacttgtctt
    50941 gccaatcgac aatgtctgtg caccagaatt ttcaaatgat gttgaaacac aagtaatcga
    51001 aggagacatt cctgatggtt tgatggcttt agacatcggc ccagcatcag taaaattatt
    51061 tgctgataca ttacaaggtg caaaaacagt tgtttggaac ggacctatgg gtgtatttga
    51121 aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa gcaatcgcta acttagaagg
    51181 cgcaacaaca atcatcggtg gcggagactc agccgcagca gcagaacaat taggatttgc
    51241 tgacaaattc acacacatct caacaggtgg cggcgcaagc ttggaattgc ttgaaggtaa
    51301 agaattacca ggactagcag caatcaacga taaataaacg accaagtttg ctcaattctg
    51361 acgagtaatc gatcaaaagg tgaagggagt tttcatgact ccctagccat ttttcaataa
    51421 attaaggagt gtgttcctca atgcgtaaac ctattatcgc aggtaactgg aaaatgaaca
    51481 aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa aaacaaaatc ccttcaaatg
    51541 acaaagttga ttcagtcatc ggatctcctg cattattctt acaagaatta atgacatcag
    51601 ctgaaggtac agaactaaaa atctcagcac aaaactgcta ctgggaaaac tcaggtgcgt
    51661 tcactggtga aacttcacca gcagctcttg ctgatctagg tgttcattat gtgatcatcg
    51721 gacattcaga acgtcgtgaa tatttccatg aaacagacga agacatcaat aaaaaagcga
    51781 aagctatctt tgcaaatggt atgacaccaa tcctatgctg tggcgaatct ttagaaacct
    51841 atgaagcagg caaaactgct gaatggatcg aaggacaaat tacagctggt ttagctgatc
    51901 tttcagctga acaagtgtca aacatggtta ttgcttatga accaatctgg gcaatcggta
    51961 ctggtaaatc agctgacgca caaatcgctg acgaaatttg tggagttgta cgtcaaacag
    52021 ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg tatccaatac ggcggttctg
    52081 taaaaccaga aaatattgct gaatacatgg caaaagaaaa tgttgacggt gctttagtcg
    52141 gtggcgcaag cttgcaagca gattcattct tgtcattgtt agacgctgta aaataattgt
    52201 tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg agcaaaatta actaaaatcg
    52261 tatatgaggg aaatccctta ggaaaaaaca aactcaaagg agagacaaaa catgtcaatt
    52321 attactgatg tttacgctcg cgagatctta gactcacgtg gtaacccaac aatcgaagta
    52381 gaagtataca cagaaagcgg agcttttggc cgcggaatgg ttccttcagg tgcttcaact
    52441 ggtgaatacg aagcagttga attacgtgac ggagacaaag ctcgttatgg cggtaaaggt
    52501 gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg aagcaatcat tggctacgat
    52561 gttcgtgacc aaatggctat cgacaaagct atgatcgctt tagacggaac tcctaacaaa
    52621 ggtaaattag gtgcgaacgc tattttaggt gtttctatcg ctgttgcacg tgcagctgct
    52681 gactacctag aagtaccttt ataccactac ctaggcggat tcaacacaaa agtattgcca
    52741 acaccaatga tgaacatcat caacggcgga tcacatgctg ataactcaat cgacttccaa
    52801 gaattcatga tcatgcctgt tggcgctcca acattcaaag aagctctacg tatgggtgct
    52861 gaagtattcc acgcattagc ttcaatcttg aaagctcgcg gattagcaac ttctgttggt
    52921 gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag aaggttttga agtaatcatc
    52981 gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg ttgttcttgc tatggatgct
    53041 gcttcttcag aattctacga caaagaaaaa ggtgtttacg tactagctga ttcaggcgaa
    53101 ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag aattagtttc taaatatcca
    53161 atcatctcaa tcgaagacgg attagacgaa aacgactggg acggattcaa gaaattaact
    53221 gacgtattag gcgacaaagt tcaattagtt ggtgacgact tgttcgtaac aaacactcaa
    53281 aaattatctg aaggtatcga aaaaggaatt gctaactcaa tccttatcaa agtgaaccaa
    53341 atcggtacat taacagaaac tttcgaagct atcgaaatgg ctaaagaagc tggctacaca
    53401 gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa caatctctga tatcgctgtt
    53461 gcaacaaacg ctggccaaat caaaactggt tctctatcac gtactgaccg tattgctaaa
    53521 tacaaccaat tactacgtat cgaagaccaa cttggtgaag ttgcagaata caaaggtttg
    53581 aaatctttct acaacttaaa aaacaaataa ttcttttcta atgaagatac atgatcgact
    53641 attgaaagat agtcgagtat gatcacttag ttaagtaatc gaactctcta cttgctgaaa
    53701 tatcagcagg tagagagttt tttttgcaac tagcttctgt atatgatgtt tacaaagaat
    53761 ggtacaagaa aagatcatcc ttgaaaaagg aaagctaaca ataactagca tcatataaga
    53821 gcttttaagg atagaacagt agaataggaa tctatgagca agtcctctcg tataaataaa
    53881 ataggaggga agtagcagca tatttttcat aaagtggctc tctttcttca tttttacttt
    53941 ggttgctcct atttgaaaat tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga
    54001 gaggattaga tgaaaaaaga agaaaaaatc gcgattttac aagagatcat ccgaattaaa
    54061 tcagtgaatg gaaacgaagg tgaagtagca gcctatttga ataagctttt ggcaaagcat
    54121 gacataacag gagagattgt ttcttacaga gatggtagag ataatttgat tgctcgttac
    54181 caaaagggcc aatcaggaaa agtgttaggc ctatctgggc atatggatgt cgtggcggct
    54241 ggtgatgaat cgtcttggac ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg
    54301 tacggacgtg gtgctacaga tatgaagagt ggcttggcag caatggtgat tgccatgatc
    54361 gaactgaaag agtcaggaaa accatttaac ggaacagtca aattattagc cactgtcgga
    54421 gaagaagtgg gtgaactagg tggggaagag ctcacgaaag caggctatgt agatgactta
    54481 gatgccttga tcatcggaga gccaactaat tacagtttga tgtatacgca catgggatca
    54541 atcaactata cagtcacctc gcacggaaaa gaagcgcata gctctatgcc agaccaaggt
    54601 tataatgcaa tcaatcacct aaatgaattc attacgaaag caaatgctga aatgaatcac
    54661 ctcgctgaaa caatcgagaa tcctgtccta ggaaaaacaa tccataatgt tacattgatc
    54721 agtggcggga atcaagtcaa cagcatccca agccatgcgc agctccaagg gaacattcga
    54781 tcgattccag aatacccaaa tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa
    54841 ttgaatcaag agacagacta tcatttggaa ttgatgatcg attacaacaa aatacctgtc
    54901 aaagctgatc cagattctcc tttgattcat tgcattcagc aacaattcag ccagccatta
    54961 cctttagttg gagcggcagc aacaacggat gcagccgaat ttacgaaagc taaccattcc
    55021 ttcgactttg tcgtctttgg gcctggtgtc gttactttgc ctcatcaagt agatgagtat
    55081 gttgaaatcg ataattattt ggatatgatt gaaaaatatc aaggaattat tttatcctac
    55141 ttagcgtaag atttttttaa taaatgaaaa aacagagcca gattagcatt attttgctaa
    55201 tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt gtggcaacat
    55261 agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt ctgtacctgc
    55321 ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata gctgaacgat
    55381 catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga tcgaagaaac
    55441 gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc tgtttttatc
    55501 tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa tttggattgg
    55561 cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc tggacaatcc
    55621 tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc cgatagaatc
    55681 gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat tgatcccaat
    55741 acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca ttttgttagc
    55801 ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt aaaagaacgg
    55861 ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta ctgttagcag
    55921 gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt ccttgagttt
    55981 ccacagcagt gaagcttttg aaaccttcac agatggcccc ctcgataaaa taagcaacag
    56041 ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg aagtcaaaat
    56101 aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga cagcagtatt
    56161 cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc atttctatcg
    56221 tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag taattgtatt
    56281 cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag acaaaacgat
    56341 ccatatgttt cccaatattt gggttgactt tcaaagcaaa agcgatattg gtcaaaggac
    56401 ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa aaatcaacgg
    56461 catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc cctaaaccat
    56521 ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt tccattcctt
    56581 gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca ttctgcgtac
    56641 ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg atatcagggg
    56701 atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa tcaataatta
    56761 cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta ttctgggaag
    56821 tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag tatactggct
    56881 tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac tttaagtaaa
    56941 tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc ctatggatcg
    57001 gtacattgga taatagttag attatagttt ggcagaaagc ggcgggtgta caatggttat
    57061 tttttatttc attcttattc tgataattat agggatcagt taccattttc tttcttattt
    57121 ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt atgaagacac
    57181 tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac gacaaaaatt
    57241 agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca aaagttatga
    57301 cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata aatgggtcat
    57361 ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag gtaaaagata
    57421 tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag aaagtgaagg
    57481 agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga tccaactcat
    57541 cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg gagcttctac
    57601 gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta ttttagacag
    57661 cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca ctgtctttcc
    57721 taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg gttactcctt
    57781 aaagcaagca tcagctacga gacagcttgg cagcaaccat ctgcctttat tggtcattca
    57841 tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa atgctacggc
    57901 aggggataaa gcactgctgc ttgttccaga agcagaacac ttggaagcat cgatgaaaga
    57961 tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa cgatgaatta
    58021 attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag atcaggagac
    58081 tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg catatggaaa
    58141 tcaaagaaga aaaaaatcgt cttgctttat tcaatgatga acaacaagag atcggagaaa
    58201 tgacatggtc agatgcgggc ccagacatca tgatcattga ccatacattt gtagatccag
    58261 catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa ttagcaagac
    58321 gagacgggaa aaagatcatt cctttatgca cttatgcaaa aaaagagttt gaaaagaaac
    58381 cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt ttgaactttc
    58441 agctgttatt ccatttatat agagttgtca cggactttta tctatgataa agttaaagag
    58501 acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga atcgtgatcg
    58561 ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa aatagtgcag
    58621 cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct cgcggctttg
    58681 aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc ttattatttg
    58741 tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg cgggcggctt
    58801 tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg taaaatgagg
    58861 cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa catggaaagc
    58921 gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga atgctcgcac
    58981 gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga cacggaacaa
    59041 tggagccata tgatattttg caagaaaaag cagaaagctg gtgggcagac actaaaaaag
    59101 ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga ttatcaatgg
    59161 gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc ggttttttct
    59221 gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa tacgtacgtc
    59281 aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca gttgcttacc
    59341 gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc gtagaaagta
    59401 ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat gaaatgatcg
    59461 accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg acacttcgat
    59521 ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa ttagaaaaag
    59581 acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga caaaagaaac
    59641 gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt tttctatgga
    59701 agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac tagtacaaac
    59761 gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa aagtgaaact
    59821 tcctttaaaa ccaatcttga tcgaaggaac atttcgagca aatgaacgag gatttggatt
    59881 tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga ctggttttgc
    59941 aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag atatggatcg
    60001 cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt tagcagggga
    60061 attcgttgct tattcagaag aagaaataca agaaacggat ctttacggcg ttgttatccc
    60121 gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag ttcgtccagt
    60181 tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt atgcgactag
    60241 tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca tggatatcct
    60301 ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat tgtctgaagc
    60361 agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag atttaagaaa
    60421 tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg cagttacggt
    60481 taaaaaatta gataacggta attattttct tggagtacac attgcagatg tttcttacta
    60541 tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa gtgtatattt
    60601 gactgatcga gtgattccga tgatcccgca acgtttatcg aatggtatct gttcactgaa
    60661 tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa atggacaagt
    60721 agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga cctacacagc
    60781 tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg ctcagctcgt
    60841 acctatgttc caagaaatgg gtgaattgca tcgaatcctt gaaacgatgc gaatcaaacg
    60901 tggcgcgatt tcttttgagg atcgcgaagc aaaaatcctt gttgatgctg aaggacaccc
    60961 aaatgatatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat ctttcatgct
    61021 tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt ttatttaccg
    61081 aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg cttcagcttt
    61141 gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac agcatgtgat
    61201 cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc taagaagcat
    61261 gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg cagattacta
    61321 cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc gtctgatccg
    61381 ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctacgg aacttcctga
    61441 aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac gtgaagtaga
    61501 ttccatgaag aaagcagaat tcatgatgga caaagtgggg gaagaattcg atgggatcat
    61561 cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag agggattgat
    61621 ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt tagctcttgt
    61681 aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa aagtagtcaa
    61741 agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag aggttacagc
    61801 gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa aacaagcagg
    61861 aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta agcaaacgtt
    61921 cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga aaaagaaaaa
    61981 ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa ttgcacaaaa
    62041 caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga tggtcttgca
    62101 aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg gatttattcg
    62161 cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg aacaaggaaa
    62221 tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa aacaaatcat
    62281 ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa aagtctatat
    62341 tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat cttatgataa
    62401 acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat taaaaaattt
    62461 ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt acttaatgat
    62521 aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt cttagatcag
    62581 gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa agtgtctaat
    62641 tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg tggttagaaa
    62701 ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc ggggaaaaga
    62761 ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt tggtttgatg
    62821 gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta gcttactttt
    62881 ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa tgggtcattg
    62941 attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc aatttccatt
    63001 tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt ttagtaacaa
    63061 aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac ccgtttgtta
    63121 ctttaacttt ggcatttata atgatcttac ttactcactt atttggggtg aaaaagttag
    63181 gcttcaaagg atatttcgta aattcgttct tgaaaccata tagttttatg tttccgatga
    63241 aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat gggaatattt
    63301 atgcggggga agttttgcta accttgattg caaaaatggt aataagctta ggctggttct
    63361 cattgccgtt agcaattcca ttagagatgg tctggatcgc attctccttg tttatcggaa
    63421 gcatccaagc atttgtcttc gttactttat ctatggttta tatgagccat aagattgaag
    63481 tggaagaata atattcgttt taaaaacaaa ctactattta tctaatttag gaggaaaaca
    63541 attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg agccggttat
    63601 ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc tgaaatgtct
    63661 ggtcaattcc gtacaacaat gtttatcggg gtagctctag tcgaagctgt tcctatctta
    63721 ggggtagttg tcgccttgat tctagttttt ggtgtttaat tgaatcagta ttaaggcaga
    63781 aacaatttgg tttgttactg cttttttaca tgaaaactag aagtacttca aagaaaggaa
    63841 ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat gttaggaaat
    63901 gtcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca ttttgcttgg
    63961 ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga tttggattct
    64021 gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca acaattgtta
    64081 gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg agaattaagc
    64141 cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag taaagcccaa
    64201 gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga tgttgcagaa
    64261 ctatctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga aatgcacgaa
    64321 tcattaatca atcaatatat cgaaggtcta ggttcttcaa atgaaactag ataaatatac
    64381 agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa ataaagcaga
    64441 agaaatttat caagatctcg taagcttacg tcaaatctat gaacaagtac ctggattagg
    64501 taatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca tggacaagct
    64561 tgttagtggc tatgatggaa tcgttaaaaa ctttttagaa gttgtttatg cgtataaccg
    64621 aatggatgat cttttgttca tgatcgaaga atatgaacgt cgatatgatg aacatcaagg
    64681 tttgctgttg ggaagtgtca caacagcagt tccactttca gacgaacaac gaatacaatt
    64741 agaaaagaat gttgcaaaaa caatgaacta tcaatcagtc gaattaaaac aaatcgttga
    64801 ttcttctatt attggtggcg cgattgttga agctaaccat cgagtaatcg acggaagcat
    64861 tcgcacgcaa ttagaaaata tgcgtaatca attaaataga tagtacaaag aggtaggtga
    64921 atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa attgaaaatt
    64981 atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt gacgggatcg
    65041 ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt tcaaatggct
    65101 catacggaat ggcgcaaaac ttagaatcaa atgatgtagg tatcattatc cttggcgatt
    65161 tcgagtccat tcgtgaagga gataaagtaa aacgaacagg aaagatcatg gaagttccag
    65221 ttggagaggc cttgattgga cgggtagtta atccgctagg tcagccaatt gacggactag
    65281 gtgaaatcgt tactgacaaa gctcgtccag tagaagcaat ggcaccaggc gttatgcaac
    65341 gtaaatctgt taacgaacca atgcaaacag gtctaaaagc aatcgacgct cttgtaccaa
    65401 tcggacgcgg ccaacgtgaa ttagttatcg gtgaccgtaa aacagggaaa acttccattg
    65461 cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat gtagcaatcg
    65521 gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat ggcgcaatgg
    65581 attacacaat cgttgtgaat gccggtgcgt ctcaaccagc gccgttgctt tatatcgcac
    65641 catatgctgg tactgcaatg ggtgaagagt tcatgtacaa cgggaaacat gtcttgatca
    65701 tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg ctgcttcgcc
    65761 gtccaccagg tcgtgaagct tatccagggg atgttttcta cttacactca cgcttattgg
    65821 aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc ttaccatttg
    65881 ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt tcgatcactg
    65941 atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca gcggttgatg
    66001 ccggactatc tgtatctcgt gtagggggtt ctgcgcaaat caaagcaatg aaaaaagttg
    66061 ctgggacttt acgtctagac ttagcaagtt accgtgaatt agaagcattt acacaattcg
    66121 gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt acagtagaaa
    66181 tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg attttgtatg
    66241 ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac ttcgaacatg
    66301 aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc cgtacaacaa
    66361 aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa gacatgttct
    66421 tagctacaaa aggaaataat tcctctacag aagataaatt gaaatccatt caaaatgcat
    66481 agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat cgcttcaacg
    66541 aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa gttgacaaaa
    66601 tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga aatcgttacg
    66661 catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg ggatatcaat
    66721 tacaacagca tgttgctcag ccgtcctgtt aagaaaacgg gttatatcgt catcactgca
    66781 gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat gtctatttta
    66841 gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg aactggtgca
    66901 gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct atctgatcag
    66961 ccaagctttg atgaagtccg caaaatcgta aatatggcaa caactatgta ccaaaacgaa
    67021 gtattcgatg aattatacgt atgttataac catcatatca attcgctaac tagtcagttt
    67081 cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac tactttcgaa
    67141 caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt gcctcagtat
    67201 gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca tgctgctggt
    67261 atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga gctgacggtt
    67321 tcttacaacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat tgttgccgga
    67381 gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag gcaagattgt
    67441 tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct taccagatat
    67501 caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg ttcttgaagc
    67561 agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa cagatggttt
    67621 acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg taggtaaaga
    67681 aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa cacctttccc
    67741 agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg aattaagtac
    67801 aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc catatttaaa
    67861 aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac tgatccaaga
    67921 actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg gtgtaggtga
    67981 acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag ttatcgaaaa
    68041 aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc gtgttgcttt
    68101 gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg tactgttgtt
    68161 tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct tgttaggacg
    68221 gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc aattgcaaga
    68281 acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct atgtgccagc
    68341 cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg cgacaaccaa
    68401 cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat tggcttcttc
    68461 ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag caacagaagt
    68521 ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct taggtatgga
    68581 tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc aattcttctt
    68641 gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat atgtccctgt
    68701 agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg atcttcctga
    68761 agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga aaacactagg
    68821 ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta actcctgccg
    68881 gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct ggtgagcttg
    68941 ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa gttcgtgtaa
    69001 aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga atcatggaag
    69061 ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt gatatcgatg
    69121 tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa gcaaaagcaa
    69181 aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca atcaatcgta
    69241 tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt taagaacagt
    69301 aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt tacgaaaatt
    69361 gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt ctggaacacc
    69421 gtatatcgga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac aaatggaaaa
    69481 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga ttgaacaccg
    69541 tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt ttgcatattt
    69601 ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta tacttgtttt
    69661 tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca atcgttagaa
    69721 aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg agtcatttgg
    69781 cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact tttttcagac
    69841 caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt ttagggtaca
    69901 ctgcaagtaa ttttttctta gaagttatcg ctttatgccg aaatcttttc gttagcctgt
    69961 aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa agattttgag
    70021 taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc atcgtaagag
    70081 gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct gttttgccta
    70141 ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac gttccgattc
    70201 tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat gtagattttg
    70261 atgaacaaaa gaatcaagta acaatcgatg cttcaagaca attagaaatc gaagcgcctt
    70321 atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta cttgccagaa
    70381 atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga ccaatcgatt
    70441 tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac ggctatatcg
    70501 aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct agtgtaggtg
    70561 ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc attgaaaacg
    70621 tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg ggcgctcaag
    70681 tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta catgcagtca
    70741 accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct gctgcaatga
    70801 cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca ttgatttcta
    70861 aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt gtcatcggac
    70921 caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc cctacagata
    70981 tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgta gtgactgaaa
    71041 cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac gctcatgtca
    71101 aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt gcagaagtat
    71161 atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa gcaaatggaa
    71221 tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc catatcaaac
    71281 tacagcaatt aggtgcagac gttgaacgtg tagacatgga tcaaacttct gctgaaaaaa
    71341 cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat atcttattaa
    71401 ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg gccggaacga
    71461 tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag ccaagtttgt
    71521 ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt ctagtcgata
    71581 tgaagtgagc catgaagcag aacatgacaa aagaaaacag aagggaacgt tgacgttagt
    71641 cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga agatattgat
    71701 ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta gctgccgtta
    71761 ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg cactaaaagg
    71821 gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag gcggtatcga
    71881 ttatgtacca ttgacattcc aattaacgcg aaataagaaa gataaaaaag aaccagatta
    71941 taaaaaggtt gtgaaaaata cgagtagcac tgagcgataa agaaacaatt tggaaaatag
    72001 cttcttatat tttgcaaaat tgtgagttat tgccgaagtg ttggattttg aacaccgtca
    72061 aaaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag gaaaattatt
    72121 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
    72181 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
    72241 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
    72301 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag tagccccaac
    72361 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
    72421 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
    72481 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
    72541 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag taattttagc
    72601 aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa aatgctttca
    72661 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
    72721 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
    72781 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
    72841 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
    72901 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
    72961 tcatggattt aatattttcc gagatttgga aactctgttt caactcccag taacgattga
    73021 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gtgattatca
    73081 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
    73141 aatcaataaa ggggcacatc tttatggtgg agaatttggg ttgatgttct taaatggcga
    73201 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
    73261 aggtctcgat aaaaattctt ataccggaaa agatgtcttt gaattagcag aaactggaga
    73321 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
    73381 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggtgttt ctgcaaaaga
    73441 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
    73501 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
    73561 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
    73621 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
    73681 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa ataaagatgt
    73741 tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt aagaattatg aagtcactca
    73801 gttatttata gggaaaaaaa tgattggtga gatcttagcc tatgggccaa aagaatatga
    73861 aattttcttt ggtgaagaag attttggaaa agaaaaaagt cttgaaaatg cgatagaaac
    73921 agtgattcga cactggaatc tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg
    73981 gttgcaaaaa tctggaaaat taggtattat aactaagtca ggttgataca aacctttcgg
    74041 aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc gttccttagg gttcagtggt
    74101 tcgaatccac tctcctccat ttcattgggg tatagccaag cggtaaggca agggactttg
    74161 actccctcat gcgttggttc gaatccagct accccagtat accacaatcg taagctctcg
    74221 ttagttcgag agctcttttt tttgctttgc taccttgttc cttgaagatt ctgctgattt
    74281 aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg aaagaaaaag
    74341 tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac aatattgaag
    74401 tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc ccaacgaatc
    74461 atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat aagtcggcat
    74521 tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa aaaattgttt
    74581 ctgacaatag gagtaggaag aacgatgtgt gtctaaatcc tctgttgatg gatagaaacc
    74641 tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa acgaaaggac
    74701 gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac caattaaaaa
    74761 atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa agagaattag
    74821 cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac ttggcagatg
    74881 aaggaatctt agaaagaaag atcggttcgg gtacatatgt tgcgcgagaa aaagtacaag
    74941 aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcgcaaaat cgagtaccgt
    75001 ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg gaaaaactaa
    75061 acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca gatgatgtcc
    75121 ctatttgttt tgaagttgcc agtgtgccac aaaagctgat cgatggctac agcaaagcag
    75181 agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag atcggtccag
    75241 cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat ttagaggtaa
    75301 aacgaggaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac ggtacgccgt
    75361 ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg gaaaaataaa
    75421 aaacaggtga tccctgattc cttacttaga aataaaagag aatcagaatc acctgttttt
    75481 atatatgatc agttaccagt tgctcgtgat aagaaacagg accctcttca aattggactt
    75541 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
    75601 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
    75661 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
    75721 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagacagca tgagtgtatg
    75781 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
    75841 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgcca gccgttccac
    75901 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
    75961 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
    76021 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
    76081 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
    76141 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
    76201 caatcgctgt agtatgcgat aataaagaca aaggtaggtg gatctgggga tgcaggaacg
    76261 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
    76321 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
    76381 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
    76441 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
    76501 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
    76561 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcgtagt
    76621 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
    76681 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
    76741 gactcggata ttttcgctgt gtccgcttct gacgcagata acaatcttgc aaataaagat
    76801 gtagatgtac tgttattagg acctcaagtt cgctttatga aagctgattt tgaaaaacgt
    76861 ctggaaccaa aaggcattcc tttagacgtc atcaatatgg ctgattatgg tatgatgaat
    76921 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
    76981 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
    77041 ataatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattaaa gcaatagact
    77101 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
    77161 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
    77221 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
    77281 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
    77341 ttactgaaaa acaaacggaa atcgcaaaga gaatactttg ccattcagaa aaaagacatc
    77401 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
    77461 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
    77521 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
    77581 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
    77641 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
    77701 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
    77761 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
    77821 acaaacttgc tttgcgcttt catcggcgtt tacttcctga acccgtcttg gttttcatga
    77881 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
    77941 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
    78001 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
    78061 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gacatactgt
    78121 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
    78181 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
    78241 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
    78301 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
    78361 gtgcagttgc tgcagtaaag atatcgtcca aaatttaaca ttcagcgaga ttattggtgt
    78421 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
    78481 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
    78541 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
    78601 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
    78661 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
    78721 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
    78781 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
    78841 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
    78901 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
    78961 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
    79021 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttagcaa gatgaattag
    79081 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
    79141 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
    79201 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
    79261 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
    79321 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
    79381 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
    79441 tggttgttga caaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
    79501 cccgtgaaac gggcatgaat actgcagatg ctactgcttt attcaatgaa gaggacgaaa
    79561 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
    79621 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
    79681 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
    79741 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
    79801 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
    79861 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
    79921 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
    79981 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
    80041 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
    80101 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
    80161 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccaca
    80221 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
    80281 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
    80341 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
    80401 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
    80461 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
    80521 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
    80581 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
    80641 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
    80701 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
    80761 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
    80821 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
    80881 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
    80941 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
    81001 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
    81061 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
    81121 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
    81181 tcgtccagat ttgctttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
    81241 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
    81301 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
    81361 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
    81421 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
    81481 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
    81541 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca cagttctact tgtcattgga
    81601 agatgatttg atgaaacgat tcggttcaga acgaatcaaa tctttcttag atcggatgaa
    81661 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
    81721 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
    81781 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
    81841 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
    81901 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
    81961 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacagctca
    82021 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
    82081 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
    82141 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
    82201 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
    82261 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
    82321 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
    82381 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
    82441 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
    82501 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
    82561 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
    82621 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
    82681 aaggtcacaa gcttcaggag gtctctttga cttagaccag ctggaagaag acattgcgga
    82741 agcagaagca aaaatggctg aacctggttt ttgggatgat agcgaagctg ctcaaaaagt
    82801 gatcagtgaa aacaatgcaa ataaagaaac ttatgatcaa ttccattctc ttgctgaaga
    82861 actggaagaa atcgaattga tggtggagat gttgcaggaa gaacctgatg cagccatgca
    82921 agaagaagca gaaacacgta tcaatgcgct agatgaaaaa atgaagacat atgaattatc
    82981 tatgttgtta gatggtcctt atgatcgaaa caacgccatc gttgaattgc atccaggggc
    83041 tggcggaacg gagtcccaag attggggcag tatgctttta cgtatgtata cacgctgggc
    83101 agagcagcat gggtatcaag tagagacact agattatcaa gcaggtgatg aagcaggaat
    83161 caagagtgtg accctactga tcaaaggaca caacgcattt ggttatctga aatcagaaaa
    83221 aggtgttcat cgccttgtac gtatctcgcc ttttgactca gcgaaacgac gtcatacttc
    83281 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
    83341 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
    83401 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
    83461 acaacgttca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
    83521 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
    83581 aatcggctgg ggttcacaga tccgttctta tgttttccat ccatattcta tggtaaaaga
    83641 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
    83701 atttattgac ggctatttaa aaatgcatct acaataagaa cccattgtta aattgaaata
    83761 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
    83821 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
    83881 ggtacgaccg ctatccgcaa tctctcaatt gtcatcgaac aaggagagtt tgtctatgtg
    83941 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
    84001 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
    84061 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
    84121 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgggat
    84181 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    84241 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatagtg
    84301 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    84361 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    84421 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    84481 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    84541 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    84601 cagtgcggta acgatcacat tgactttagt cggtgtattc atggcagtta tcatgaacgc
    84661 aactaaatta gctcaagata tagaaggaaa cgttgatgtc tctgtattcg tagatatcgg
    84721 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    84781 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    84841 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    84901 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    84961 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    85021 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    85081 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    85141 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    85201 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    85261 caatccatca ttattacgtt cccattattc attgattcat ccacaaaata tcgtttggaa
    85321 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    85381 tatgagacgt ttcttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    85441 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    85501 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    85561 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    85621 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatgggtg ctcgttttga
    85681 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    85741 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    85801 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    85861 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    85921 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    85981 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    86041 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    86101 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    86161 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    86221 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    86281 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    86341 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    86401 atacaaaaga agtaggctat tctcccaaat tttttccgtc cattcactct actggtgata
    86461 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    86521 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    86581 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    86641 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    86701 cagagctaat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    86761 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    86821 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    86881 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    86941 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    87001 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    87061 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    87121 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    87181 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    87241 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    87301 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    87361 tcttaccaat aataggatta gctattcttt tatcaggctg ttcaaagtgg attgatcgag
    87421 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    87481 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    87541 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    87601 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    87661 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    87721 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    87781 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    87841 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    87901 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    87961 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    88021 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    88081 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    88141 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    88201 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    88261 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    88321 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    88381 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    88441 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    88501 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    88561 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    88621 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    88681 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    88741 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gactgggttt ggtatcttag
    88801 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    88861 cattaaaggc agtgccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    88921 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    88981 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    89041 cagcattgat gccaacaggt cttactactc ctgcttctac attgacgtcc atcttaacaa
    89101 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    89161 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    89221 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    89281 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    89341 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg
    89401 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    89461 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    89521 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    89581 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    89641 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    89701 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    89761 aacagtgata aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    89821 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    89881 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    89941 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    90001 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg attattgctg ttttactgtt
    90061 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    90121 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    90181 gagaaagaag tagcactata tacggatgat ctacatgtct ggtatggcga caatgaagca
    90241 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    90301 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    90361 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    90421 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    90481 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    90541 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    90601 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgtattgct
    90661 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    90721 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    90781 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    90841 tatttaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    90901 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    90961 aaagagatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    91021 gggatcgatc taagcttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    91081 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    91141 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    91201 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    91261 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    91321 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    91381 cataaaagtg ctttatcttt atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt
    91441 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    91501 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    91561 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    91621 ggtcacttga tcgaatacaa tgatacgaag aagatattca tgaatccaga aaaacaagag
    91681 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    91741 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    91801 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    91861 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    91921 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    91981 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    92041 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    92101 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    92161 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    92221 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    92281 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    92341 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    92401 taaactgaag ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg
    92461 tgaaaaagct gttctgcatc aagtaataag aacagtcaaa caaaaaatag ctcctcatat
    92521 ttttcgtttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggga
    92581 aagggtttgt gacaaaacat gtcacaaacc ctttt
//
LOCUS       DACBZN010000003        80298 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.003, whole genome shotgun sequence.
ACCESSION   DACBZN010000003 DACBZN010000000
VERSION     DACBZN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.003"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(3..872)
                     /locus_tag="IVD64_000180"
     CDS             complement(3..872)
                     /locus_tag="IVD64_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6147462.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(968..2272)
                     /locus_tag="IVD64_000181"
     CDS             complement(968..2272)
                     /locus_tag="IVD64_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6147463.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(2269..2778)
                     /locus_tag="IVD64_000182"
     CDS             complement(2269..2778)
                     /locus_tag="IVD64_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147464.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(2953..5088)
                     /locus_tag="IVD64_000183"
     CDS             complement(2953..5088)
                     /locus_tag="IVD64_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6147465.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(5171..6826)
                     /locus_tag="IVD64_000184"
     CDS             complement(5171..6826)
                     /locus_tag="IVD64_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6147466.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(6828..7142)
                     /locus_tag="IVD64_000185"
     CDS             complement(6828..7142)
                     /locus_tag="IVD64_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6147467.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            7312..7572
                     /locus_tag="IVD64_000186"
     CDS             7312..7572
                     /locus_tag="IVD64_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP6147468.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            7586..7900
                     /locus_tag="IVD64_000187"
     CDS             7586..7900
                     /locus_tag="IVD64_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP6147469.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(8630..9184)
                     /locus_tag="IVD64_000188"
     CDS             complement(8630..9184)
                     /locus_tag="IVD64_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP6147470.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(9310..11239)
                     /locus_tag="IVD64_000189"
                     /pseudo
     CDS             complement(9310..11239)
                     /locus_tag="IVD64_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(11545..12261)
                     /locus_tag="IVD64_000190"
     CDS             complement(11545..12261)
                     /locus_tag="IVD64_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6147471.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(12262..14085)
                     /gene="glpO"
                     /locus_tag="IVD64_000191"
     CDS             complement(12262..14085)
                     /gene="glpO"
                     /locus_tag="IVD64_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP6147472.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(14099..15595)
                     /gene="glpK"
                     /locus_tag="IVD64_000192"
     CDS             complement(14099..15595)
                     /gene="glpK"
                     /locus_tag="IVD64_000192"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP6147473.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(15731..17191)
                     /locus_tag="IVD64_000193"
     CDS             complement(15731..17191)
                     /locus_tag="IVD64_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6147474.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(17181..18476)
                     /locus_tag="IVD64_000194"
     CDS             complement(17181..18476)
                     /locus_tag="IVD64_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6147475.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(18808..19485)
                     /locus_tag="IVD64_000195"
     CDS             complement(18808..19485)
                     /locus_tag="IVD64_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP6147476.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(19515..20606)
                     /locus_tag="IVD64_000196"
     CDS             complement(19515..20606)
                     /locus_tag="IVD64_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP6147477.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(20707..21141)
                     /locus_tag="IVD64_000197"
     CDS             complement(20707..21141)
                     /locus_tag="IVD64_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6147478.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(21725..23182)
                     /gene="ascB"
                     /locus_tag="IVD64_000198"
     CDS             complement(21725..23182)
                     /gene="ascB"
                     /locus_tag="IVD64_000198"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6147479.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(23179..25074)
                     /locus_tag="IVD64_000199"
     CDS             complement(23179..25074)
                     /locus_tag="IVD64_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6147480.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(25149..26876)
                     /locus_tag="IVD64_000200"
     CDS             complement(25149..26876)
                     /locus_tag="IVD64_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6147481.1"
                     /translation="MDLNKRQLQILVVLRNKNHWITSEELAEALGTNKKTIQTEIKNM
                     LAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRASTIVTYLLFQ
                     KGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLKIIAEENSKRI
                     FLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSGEDFIRFSRYL
                     VLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIENRLLELNYLM
                     INDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTRMDAGHNTMNH
                     FVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFRKQGEILIVSN
                     QPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTTEQELIFEWDD
                     LIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKIHKKIDSIQQL
                     ISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKRVFLSPLTIRE
                     TIYLFSFKQ"
     gene            complement(26956..28452)
                     /locus_tag="IVD64_000201"
     CDS             complement(26956..28452)
                     /locus_tag="IVD64_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6147482.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(28646..30268)
                     /locus_tag="IVD64_000202"
     CDS             complement(28646..30268)
                     /locus_tag="IVD64_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6147483.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(30596..31909)
                     /locus_tag="IVD64_000203"
     CDS             complement(30596..31909)
                     /locus_tag="IVD64_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6147484.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(32154..32378)
                     /locus_tag="IVD64_000204"
     CDS             complement(32154..32378)
                     /locus_tag="IVD64_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147485.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(32398..32766)
                     /locus_tag="IVD64_000205"
     CDS             complement(32398..32766)
                     /locus_tag="IVD64_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6147486.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(32963..33673)
                     /locus_tag="IVD64_000206"
     CDS             complement(32963..33673)
                     /locus_tag="IVD64_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147487.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(33691..34188)
                     /locus_tag="IVD64_000207"
     CDS             complement(33691..34188)
                     /locus_tag="IVD64_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6147488.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(34225..34677)
                     /locus_tag="IVD64_000208"
     CDS             complement(34225..34677)
                     /locus_tag="IVD64_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6147489.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(34812..35417)
                     /locus_tag="IVD64_000209"
     CDS             complement(34812..35417)
                     /locus_tag="IVD64_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6147490.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(35867..36487)
                     /locus_tag="IVD64_000210"
     CDS             complement(35867..36487)
                     /locus_tag="IVD64_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6147491.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(36489..37358)
                     /locus_tag="IVD64_000211"
     CDS             complement(36489..37358)
                     /locus_tag="IVD64_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP6147492.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(37381..37788)
                     /locus_tag="IVD64_000212"
     CDS             complement(37381..37788)
                     /locus_tag="IVD64_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6147493.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(37807..39096)
                     /locus_tag="IVD64_000213"
     CDS             complement(37807..39096)
                     /locus_tag="IVD64_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6147494.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(39123..39425)
                     /locus_tag="IVD64_000214"
     CDS             complement(39123..39425)
                     /locus_tag="IVD64_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6147495.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(39450..41543)
                     /locus_tag="IVD64_000215"
     CDS             complement(39450..41543)
                     /locus_tag="IVD64_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6147496.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(42032..42796)
                     /locus_tag="IVD64_000216"
     CDS             complement(42032..42796)
                     /locus_tag="IVD64_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP6147497.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(42907..43776)
                     /gene="mdcB"
                     /locus_tag="IVD64_000217"
     CDS             complement(42907..43776)
                     /gene="mdcB"
                     /locus_tag="IVD64_000217"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP6147498.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(43801..44877)
                     /locus_tag="IVD64_000218"
     CDS             complement(43801..44877)
                     /locus_tag="IVD64_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP6147499.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(44901..45494)
                     /locus_tag="IVD64_000219"
     CDS             complement(44901..45494)
                     /locus_tag="IVD64_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP6147500.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(45498..47174)
                     /gene="mdcD"
                     /locus_tag="IVD64_000220"
     CDS             complement(45498..47174)
                     /gene="mdcD"
                     /locus_tag="IVD64_000220"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP6147501.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(47164..47463)
                     /locus_tag="IVD64_000221"
     CDS             complement(47164..47463)
                     /locus_tag="IVD64_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP6147502.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(47565..49211)
                     /gene="mdcA"
                     /locus_tag="IVD64_000222"
     CDS             complement(47565..49211)
                     /gene="mdcA"
                     /locus_tag="IVD64_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP6147503.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(49218..50129)
                     /locus_tag="IVD64_000223"
     CDS             complement(49218..50129)
                     /locus_tag="IVD64_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6147504.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            50249..51106
                     /locus_tag="IVD64_000224"
     CDS             50249..51106
                     /locus_tag="IVD64_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6147505.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(51293..51655)
                     /locus_tag="IVD64_000225"
     CDS             complement(51293..51655)
                     /locus_tag="IVD64_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147506.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            52001..53440
                     /locus_tag="IVD64_000226"
     CDS             52001..53440
                     /locus_tag="IVD64_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6147507.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(53447..55999)
                     /locus_tag="IVD64_000227"
     CDS             complement(53447..55999)
                     /locus_tag="IVD64_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP6147508.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(56113..57444)
                     /locus_tag="IVD64_000228"
     CDS             complement(56113..57444)
                     /locus_tag="IVD64_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6147509.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(57410..58888)
                     /locus_tag="IVD64_000229"
     CDS             complement(57410..58888)
                     /locus_tag="IVD64_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6147510.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(59193..61028)
                     /gene="tdc"
                     /locus_tag="IVD64_000230"
     CDS             complement(59193..61028)
                     /gene="tdc"
                     /locus_tag="IVD64_000230"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6147511.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            61498..62925
                     /locus_tag="IVD64_000231"
     CDS             61498..62925
                     /locus_tag="IVD64_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6147512.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(63017..63382)
                     /locus_tag="IVD64_000232"
     CDS             complement(63017..63382)
                     /locus_tag="IVD64_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6147513.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(63379..63744)
                     /locus_tag="IVD64_000233"
     CDS             complement(63379..63744)
                     /locus_tag="IVD64_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6147514.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(63936..64184)
                     /locus_tag="IVD64_000234"
     CDS             complement(63936..64184)
                     /locus_tag="IVD64_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147515.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            64183..64311
                     /locus_tag="IVD64_000235"
     CDS             64183..64311
                     /locus_tag="IVD64_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP6147516.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            64368..66062
                     /locus_tag="IVD64_000236"
                     /pseudo
     CDS             64368..66062
                     /locus_tag="IVD64_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
     gene            66066..66185
                     /locus_tag="IVD64_000237"
                     /pseudo
     CDS             66066..66185
                     /locus_tag="IVD64_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            66267..66458
                     /locus_tag="IVD64_000238"
     CDS             66267..66458
                     /locus_tag="IVD64_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147517.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            66836..67969
                     /locus_tag="IVD64_000239"
     CDS             66836..67969
                     /locus_tag="IVD64_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP6147518.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            68000..68320
                     /locus_tag="IVD64_000240"
     CDS             68000..68320
                     /locus_tag="IVD64_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147519.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(68349..68813)
                     /locus_tag="IVD64_000241"
     CDS             complement(68349..68813)
                     /locus_tag="IVD64_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6147520.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(68945..70199)
                     /locus_tag="IVD64_000242"
                     /pseudo
     CDS             complement(68945..70199)
                     /locus_tag="IVD64_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(70228..70545)
                     /locus_tag="IVD64_000243"
     CDS             complement(70228..70545)
                     /locus_tag="IVD64_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6147521.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(70571..70891)
                     /locus_tag="IVD64_000244"
     CDS             complement(70571..70891)
                     /locus_tag="IVD64_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6147522.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(71355..72770)
                     /locus_tag="IVD64_000245"
     CDS             complement(71355..72770)
                     /locus_tag="IVD64_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6147523.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(72787..74103)
                     /locus_tag="IVD64_000246"
     CDS             complement(72787..74103)
                     /locus_tag="IVD64_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6147524.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(74253..74951)
                     /locus_tag="IVD64_000247"
     CDS             complement(74253..74951)
                     /locus_tag="IVD64_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP6147525.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(75240..76229)
                     /locus_tag="IVD64_000248"
     CDS             complement(75240..76229)
                     /locus_tag="IVD64_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP6147526.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(76309..77142)
                     /gene="msrA"
                     /locus_tag="IVD64_000249"
     CDS             complement(76309..77142)
                     /gene="msrA"
                     /locus_tag="IVD64_000249"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6147527.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(77317..78945)
                     /locus_tag="IVD64_000250"
     CDS             complement(77317..78945)
                     /locus_tag="IVD64_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147528.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(78932..80266)
                     /locus_tag="IVD64_000251"
     CDS             complement(78932..80266)
                     /locus_tag="IVD64_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147529.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        23577 a        16847 c        13499 g        26375 t
ORIGIN      
        1 ttttatttct ttttgcggat gattccgtaa gctgctgcga ctaacacacc cgcccctgct
       61 aaggaataac tgacagaagc tgttttttca tttgtttttg gcaaagtctg acttgttgat
      121 ttggatggct ctgtcgtttg ctgaactgtt tctgtcgtgc ttccagaggt cactgatttt
      181 tctcctgccg aatgcaaaat atcttttatt cgttggaaac tagctgctaa gtcagctttt
      241 tttgcttcaa aatcattagc aactgtttgt gcttcgctga tctttccgtg gattgcttga
      301 ctggccgctg tgatatccgc ttgagtttga cttagctttc cagaagctgt ttgagcaacc
      361 tgatttaatt gctgattgaa ttcttttgct tgttggatct tcccgtcaag atctgtttgg
      421 acagcttccg cttttgcttt cgcttcggca agctttttct caagttgtgc acggtcattt
      481 cccactttat ttttaagatc agctaaatct tgatcgattg ctgcttggtc ttctttcaat
      541 ttgttttttg cagcttctaa tgcttcttcg agttcttttc ttttattttc caagtcttgc
      601 aaatgatcga tttggtcaga agaggtagaa ctatttattg atgtatcttc tgaagatgaa
      661 atcgtttctc ctgaattatc tgtcggtaca ttggaagtca tttctgtttc cgaggcaccg
      721 ttttcagcac cagaagagct tatggttgtt gaaggttctg tttgagcagg ttcagaagtt
      781 tccgcaaaag ctgtctgtcc caataagaaa atactggaaa cagcaattgg ccagattaac
      841 ggcttcattc tttttttcat cacttttccc atccttacta ctttttaact aatttcttat
      901 tataagcaaa aatgtttgga agaaccataa acacaacagg ctgtttgtct aaaaaacggt
      961 gcatctttta tttttttgta cgttccgggc ttttccatgt cccgcttcca taacctaaaa
     1021 tcgttttgac tgctggaatg aaagtcgtca cgatcgttat cgcgttcact atccagaaca
     1081 aaagcatata tagtggtgcg aaaagaaggt atttcaattt tcttcgtcga tcatctacaa
     1141 tcagcgaagc tagcaactgg aaaaatcctg cgaccatttc aaagcagatg aacacaaaac
     1201 tgaatgtaaa agtaatataa atctgttcgt agtttccttg atatacataa tgcccgatta
     1261 atccagcaaa aagaactacg gaaatacaaa agaatattga ccagacaata ctcaatgttt
     1321 gatcaatgaa gatgatcgtt cttccaatat gtttaaaagg atggagcatc acttttttga
     1381 aattcgtcag ccagacttct gttccacctt ttgcccaacg ttttctgtgt cgatatagca
     1441 tcttcaacgt cacaggtact tccatgaaaa agataatctc aggagcaaaa acagataacc
     1501 agtcatttaa ctgatgatcc caagcaatac tgatgtcttc tgtcgctcga tcttgtctga
     1561 atccgcccac atcgatcaag gcttcttttc gatacatcgt atttgctcca ctgtacgcat
     1621 aaagaccacc gaaaacagct gactgtgttc gtttaatgat gcccacgata cttgagaatt
     1681 caaccgtttg agattttgca atcaatttgg tacgattttg gacatccata tttgcagtga
     1741 ccgcagcaat atgtcttgca ccagggcgaa tgaaataatt cacataccta atcagtgcat
     1801 ctggctccgg taccgtgtct gcatcattgc ttaaaatcaa tttccctttt gcaaaggcaa
     1861 gtccgatatt aaacgcgtgg gcttttcctt tatttttttc gatccgaacc acacgaagat
     1921 tagcgtattt tttcatcagt ctagctaata tttctggtgt ttggtccgtt gaaccatcgt
     1981 ctgtcactaa tacctcatag ttgtgataat tcaatttcgt catcaaatac tcgattgtat
     2041 attcgatcac aatttcttca ttatgggctg gcaccatgat cgtgatgaac ggttcgacac
     2101 tcaagggaac atctacccat tctttttgct tatgtttgta taaaaacaca taacaccaga
     2161 caccaataaa ccatacaaat ccgcctaaaa tcggataaga aacgagaaaa aaatacataa
     2221 tagacaaaca agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc
     2281 caccccattt tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga
     2341 gacagaataa aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac
     2401 ttcttcaagt aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt
     2461 tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa
     2521 actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa
     2581 aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca
     2641 tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt
     2701 taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc
     2761 tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc
     2821 atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa
     2881 acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt
     2941 tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat
     3001 cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa
     3061 gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc
     3121 taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg
     3181 ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa
     3241 caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga
     3301 tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc
     3361 gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa
     3421 acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc
     3481 tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt
     3541 ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac
     3601 aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca
     3661 agcataagct gagccatatc gtttttcata aattctttca aacaactgat gctcatccac
     3721 ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct
     3781 tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa
     3841 ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt
     3901 caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat
     3961 caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac
     4021 tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc
     4081 tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac
     4141 attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag
     4201 ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag
     4261 gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga
     4321 tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt
     4381 tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat
     4441 tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg
     4501 gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac
     4561 caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt
     4621 ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg
     4681 tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat
     4741 ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg
     4801 acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt
     4861 catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa
     4921 gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc
     4981 tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata
     5041 cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct
     5101 ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt
     5161 ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag
     5221 aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta
     5281 gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat
     5341 ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt
     5401 ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca
     5461 attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta
     5521 gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg
     5581 gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat
     5641 ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga
     5701 tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata
     5761 catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg
     5821 atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc
     5881 gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa
     5941 gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg
     6001 cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc
     6061 ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa
     6121 taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt
     6181 caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt
     6241 ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca
     6301 ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg
     6361 caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat
     6421 ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa
     6481 caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac
     6541 cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg
     6601 gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt
     6661 cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat
     6721 acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag
     6781 cagcagacat tccacctgct acacctccga caataacgat tttcatctta tttccctcct
     6841 actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat
     6901 ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg
     6961 gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct
     7021 tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc
     7081 aaaagctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc
     7141 attttctgca cctcatttac atttttataa atacctcatg gggtatatta aacaggagct
     7201 ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata
     7261 aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt
     7321 gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa
     7381 aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt
     7441 tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag
     7501 aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt
     7561 attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa
     7621 atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat
     7681 tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt
     7741 cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat
     7801 ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt
     7861 tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga
     7921 agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt
     7981 gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     8041 agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     8101 gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga
     8161 aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca
     8221 ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt
     8281 aaaaatggat ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
     8341 ataccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa
     8401 cggaaaaata gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt
     8461 gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat
     8521 ttcaaaaaca acttctcctg tttttggttc ggttggctta ttatcgagaa gtagctactg
     8581 ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt
     8641 gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga
     8701 tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga
     8761 tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt
     8821 tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga
     8881 atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa
     8941 gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat
     9001 atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc
     9061 ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac
     9121 ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt
     9181 tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa
     9241 taaaaccaat gatttcactg tttttatttt tctttacaga cactgatatt atgagctaat
     9301 ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat
     9361 ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt
     9421 acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc
     9481 tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata
     9541 tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt
     9601 gacaaatatc cgatcacctt ttgcaaagag ctgacgttct tttttattct ccttcctatt
     9661 tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa
     9721 gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa
     9781 agcttggctt tctcctgttt cttccaaccc gcaactgata tttaggacga agtgcttccc
     9841 gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat ttcttgattt
     9901 ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat tccatgattg
     9961 cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca tctaaaagta
    10021 ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata tgaccagaaa
    10081 atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa atctcatcaa
    10141 agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc gccatcaaag
    10201 tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct ccggtgataa
    10261 tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc tcaggtaaaa
    10321 atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta ggtgtgcttc
    10381 cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc gtgatcgttt
    10441 cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat gccgccagga
    10501 cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca ttcaatttag
    10561 aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg ataatcgttc
    10621 catctatttt atttttatag ctggctttga taggaatcgt atagtattcg cctttttgaa
    10681 caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat ttcagtaatt
    10741 ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc aaattacgtg
    10801 acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct tctacattta
    10861 tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat tgattcttat
    10921 caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg atcaaatcgg
    10981 ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc ctcggcaacc
    11041 ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt gtttcttctt
    11101 gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt cgttttttgc
    11161 tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat tgtaattgac
    11221 taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc ccaaaataaa
    11281 taagggccta gagataaata aaaggataga acacagctta aaaaagtcat gtcctatcct
    11341 tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca caccaaataa
    11401 actttgttta ttggctattc gacttactga ttagatacca gctacactca caaaacatac
    11461 cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca ttttggttgt
    11521 caaactttag tatgttttat agatttacac cattaatgaa tagacaccta ccgcgataat
    11581 tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg aagttccttt
    11641 attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag cagggttgat
    11701 tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc cacctacagc
    11761 gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc caagaactaa
    11821 tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta tagcaggacc
    11881 tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat gtggcaaata
    11941 agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa tgatgaaagg
    12001 aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga ctgcaggatt
    12061 caaatgagct gggcccatga atccagaaat atagacagcc attgttacag cgagtcccca
    12121 accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta aattcacagc
    12181 cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact cccccatgat
    12241 ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat ctgattctgc
    12301 aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc attcaaaata
    12361 tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc gttcaaacaa
    12421 cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt cttcttctaa
    12481 accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg ccatttcttt
    12541 tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat aagtagcatc
    12601 ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg tttcttctaa
    12661 tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga tttctttcgt
    12721 ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc cttctgccat
    12781 cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg gttctctttc
    12841 taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt tttccaaatg
    12901 gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt cacttactgt
    12961 atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac cattgtaatc
    13021 agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt cgatatcatt
    13081 caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat aatctacatc
    13141 ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg tcgtaccaaa
    13201 gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct ttcctgtatc
    13261 gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat ggatcccttt
    13321 agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta ctttatctac
    13381 ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt catctgtcag
    13441 caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc caaccgcttt
    13501 cattttactt actagataag ccccatcttc ggcagccttt ttgatattat caatcactaa
    13561 acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc cttctttttt
    13621 caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct catatttggt
    13681 tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg agaacatatt
    13741 gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg gttttgggat
    13801 gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta ctacttccac
    13861 atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc gagaggaagt
    13921 cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg ctgctgactg
    13981 gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca attcttcttg
    14041 ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga tttcctcctt
    14101 attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt ttttctcggc
    14161 gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg cggatctcgt
    14221 ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct agcgccgttg
    14281 tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg aattgcatca
    14341 gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg attccagtgt
    14401 catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt aaagtagctt
    14461 taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct cgtgctgcct
    14521 gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc tcatcttcgc
    14581 ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt tgaagtccat
    14641 ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa gcatagtaaa
    14701 cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat aattgcggtt
    14761 tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg accatgcctg
    14821 gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc cctgaaattg
    14881 gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct gagttagaag
    14941 tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc tgatcccaat
    15001 ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca gtaacatgtg
    15061 cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac atcaattcgc
    15121 ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt atcttcgttg
    15181 ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct tgatagcctt
    15241 cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca actgcatgat
    15301 agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg ttcgttatgc
    15361 caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg gcaataactg
    15421 actgtacaga attccagatt tcatttgcat catgttccac ccaactttct tgcgggaaat
    15481 attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca aagataattg
    15541 ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct gtcattgtga
    15601 ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac tgttttaaaa
    15661 aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt attatgaagc
    15721 tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc aatcgaataa
    15781 tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg tttctctggc
    15841 aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc tcaataccgc
    15901 cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa atcggcagac
    15961 tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc atcaaaagaa
    16021 cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt ttcctcggca
    16081 attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa tgcccgagca
    16141 ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt ccaacaaaga
    16201 aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct tctttgattt
    16261 tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta tctaaaacag
    16321 cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct tcgtctacta
    16381 tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc agttctgctg
    16441 ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc cggtccgact
    16501 gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc aatagtcttt
    16561 ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta aacgttgttt
    16621 ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt tgcaaaaaaa
    16681 gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat cgtatctgta
    16741 gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct aagagctggt
    16801 tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct gtcgctaatc
    16861 gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac ttcaagcttt
    16921 cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac acaagcagcg
    16981 cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca atcaattcaa
    17041 tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct ctcaaaccat
    17101 tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc tgtaactgct
    17161 cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt tgaccacacc
    17221 atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa ctgttcaaac
    17281 gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat ctcttttttg
    17341 ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt gagtttaact
    17401 ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat tttttgggga
    17461 taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc aaaccactga
    17521 ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat tgtcttgctg
    17581 acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt ttcatttgtt
    17641 ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg agacgtttgc
    17701 aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc ttcccacatc
    17761 tgattgtcgg gacgagaatg attagccagc aaaacaagat catagttctc ctttcttttt
    17821 tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc ttctaaaaaa
    17881 agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc aggatctaga
    17941 taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt tcttgatgcc
    18001 aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat tttctttgca
    18061 ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg gtgaataagc
    18121 gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag aatcaaacga
    18181 tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa tcctattact
    18241 gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt cgtgatccaa
    18301 gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc gctgggaatc
    18361 catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat caattggcga
    18421 agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat gttcatctgc
    18481 attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac acagtattca
    18541 taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct ctccatcata
    18601 ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag agtatcattt
    18661 ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc ttttacgtac
    18721 ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg tgtaatgtaa
    18781 tagttagaat agacacatca tttattttta attctttaat gcttcaacaa ctgcttcgcc
    18841 taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa tttgaaccac
    18901 ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag catcttctgc
    18961 taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac tgaacccagt
    19021 cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca acaatcctac
    19081 aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg aagcgacttt
    19141 cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag cataataaac
    19201 cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca aggcccgatt
    19261 tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca aaacactttg
    19321 cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga tcccattttt
    19381 tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag gacgctcgat
    19441 cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc catcctcttt
    19501 ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg gcttggcaaa
    19561 taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc acggttttcc
    19621 aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc tgcaagttct
    19681 gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac accaactaaa
    19741 gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc gttgtattta
    19801 tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc tagttttgac
    19861 tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg tgtttcatat
    19921 tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg ttcaaccgct
    19981 gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa gctaccgccc
    20041 cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa atacgtattt
    20101 gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt ggctttgcca
    20161 aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt ttccgcatca
    20221 ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa agttgctcga
    20281 cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc tccttggatt
    20341 gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct gagttcacct
    20401 ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc accaagacct
    20461 ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt gaaaaagctg
    20521 agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc tgaaggtaaa
    20581 gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc tgattaacct
    20641 atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta atttgcttaa
    20701 aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg ctaattcttt
    20761 acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc agctaccatc
    20821 gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca agcgaccttc
    20881 gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag caatcgttga
    20941 tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt tttcttttaa
    21001 tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg cttcatcagc
    21061 taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt catctaacgc
    21121 tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat agtatgattt
    21181 ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat ttgaaaaaaa
    21241 gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc tggaacaccg
    21301 tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat tttgtgaaaa
    21361 tggctttcca catttttgca taaatttggc ttaataccga aaaactggct tttgaacacc
    21421 gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac agccaaacaa
    21481 aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc tgattgtaca
    21541 ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga gcaatttcaa
    21601 aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt ggcaggagaa
    21661 tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc cttatttcct
    21721 tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca aatgattttt
    21781 tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag atatatccat
    21841 aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc cacgacgtat
    21901 aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata tgctgcttca
    21961 aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg tctatagctc
    22021 cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat tgattcaaag
    22081 caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga agatacggat
    22141 ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct ggatgagcac
    22201 tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag gctgctaaga
    22261 tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac attcgttgcg
    22321 tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa gttctttgct
    22381 gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga agctgatagc
    22441 tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt gtagctaaag
    22501 cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt
    22561 tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg atctcattga
    22621 aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca gcgaaacgaa
    22681 cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc aaatataacg
    22741 gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga cattcgtcaa
    22801 acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca ccatgaggaa
    22861 aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca gcaaataaac
    22921 ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga taataatact
    22981 tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc cattgatctg
    23041 tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca gataatcctt
    23101 tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg ccccataaaa
    23161 atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc ttgtttgatt
    23221 atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa caataaccat
    23281 tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca tcaataaatc
    23341 gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc catttaaatt
    23401 cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga ctaccccaat
    23461 tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acagaactgt agatttttcc
    23521 gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg ttggatcgtt
    23581 tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta tatcctttga
    23641 ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac aggaactgat
    23701 catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat aaatcgggaa
    23761 agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac cagctactcc
    23821 accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt taagagtcac
    23881 accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag ataaagataa
    23941 actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc ctgcttgcgc
    24001 agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag aaagattgtt
    24061 ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt gccaaaatcc
    24121 gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat atccagctgc
    24181 aatcagctga cttgcataat ctgcaattgg acctacaact aataaaataa ttgggaccat
    24241 cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca agcttttgcg
    24301 aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa taaccgtact
    24361 tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc tatcactata
    24421 tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga tgaaacgatc
    24481 tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat agaaaaatgc
    24541 atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata aattcaaact
    24601 cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc ctaaagtcgg
    24661 ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt tctcagttcc
    24721 tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata cgtcatacac
    24781 ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga ctacagtgat
    24841 cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt ttagggtaaa
    24901 gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg
    24961 gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt taattaatgc
    25021 cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct ccattcaatt
    25081 agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta cttagccgag
    25141 caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt caaaggagac
    25201 aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct
    25261 gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac
    25321 ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta
    25381 tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt
    25441 tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca
    25501 tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta
    25561 gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt
    25621 tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta
    25681 ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga
    25741 ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat
    25801 tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg
    25861 atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt
    25921 ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa
    25981 aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca
    26041 tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga
    26101 ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga
    26161 ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa
    26221 acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata
    26281 agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt
    26341 tgttgaggca accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt
    26401 ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct
    26461 aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt
    26521 tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat
    26581 aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc
    26641 acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa
    26701 cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt tttgatttca
    26761 gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa
    26821 tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc
    26881 ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt
    26941 gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg
    27001 gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc
    27061 atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc
    27121 cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat
    27181 tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat
    27241 tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg
    27301 taattcataa cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg
    27361 ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc
    27421 gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa
    27481 tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg
    27541 tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat
    27601 gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg
    27661 atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc
    27721 atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg
    27781 acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc
    27841 agggaatgta accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt
    27901 aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa
    27961 ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa
    28021 gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt
    28081 gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg
    28141 aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa
    28201 acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag
    28261 tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg
    28321 ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca
    28381 tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat
    28441 ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg
    28501 attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct
    28561 actcttcatt tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca
    28621 ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc
    28681 gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta
    28741 aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc
    28801 attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta
    28861 attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt
    28921 acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat
    28981 ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc
    29041 attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct
    29101 cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg
    29161 cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat
    29221 aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat
    29281 acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata
    29341 aactattcca tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt
    29401 cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta
    29461 tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa taaagtttag
    29521 cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag
    29581 ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt gtgatttctt
    29641 tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat
    29701 cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca
    29761 aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact
    29821 gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat
    29881 cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc
    29941 acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt
    30001 tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg
    30061 cttgataatt actgatatca tacccattgt catcattcgg agactcgtat actggagaaa
    30121 gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca
    30181 aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa
    30241 ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt
    30301 tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta
    30361 ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac
    30421 tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa
    30481 acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact
    30541 attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt
    30601 tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag
    30661 atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg
    30721 caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga
    30781 tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta
    30841 ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga
    30901 acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa
    30961 cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac
    31021 ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt
    31081 ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag
    31141 tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat
    31201 gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag
    31261 gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga
    31321 tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca
    31381 accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga
    31441 tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg
    31501 catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga
    31561 tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc
    31621 ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc
    31681 caattggata acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag
    31741 tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa
    31801 ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg
    31861 tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc
    31921 ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt
    31981 gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat
    32041 ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat
    32101 tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag
    32161 tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca
    32221 ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc
    32281 ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga
    32341 tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca
    32401 atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa
    32461 agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt
    32521 ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa
    32581 cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac
    32641 aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc
    32701 tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg
    32761 tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat
    32821 tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag
    32881 tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaaacgg caatgaatgc
    32941 cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt
    33001 gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct
    33061 gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg
    33121 tttcttccgt cagcatcgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc
    33181 cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta
    33241 aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt
    33301 cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta
    33361 caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa
    33421 tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa
    33481 tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct
    33541 cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat
    33601 ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta
    33661 ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc
    33721 tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt
    33781 acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt
    33841 ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg
    33901 tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc
    33961 agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac
    34021 taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc
    34081 ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa
    34141 tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc
    34201 gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt
    34261 ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc
    34321 ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa
    34381 aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt
    34441 atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt
    34501 tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag gattttcgca
    34561 aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt
    34621 gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc
    34681 atcccacctt tattttgttt gtaagactag ttcacctctt tttgttgcaa atgcaactat
    34741 tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt
    34801 tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc
    34861 ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg
    34921 gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca
    34981 agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct
    35041 cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt
    35101 tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt
    35161 ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt
    35221 ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt
    35281 cctatttcac tgattagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca
    35341 aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga
    35401 gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa
    35461 gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag
    35521 aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc
    35581 gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt
    35641 cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa
    35701 cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct
    35761 gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt
    35821 aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat
    35881 tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta
    35941 catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct
    36001 ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac
    36061 catagtgata atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt
    36121 accgacgttg ccataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc
    36181 cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg
    36241 acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat
    36301 aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg
    36361 catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta
    36421 gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact
    36481 ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc
    36541 ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac
    36601 ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc
    36661 tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct
    36721 ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc
    36781 agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca
    36841 atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta
    36901 tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt
    36961 tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg
    37021 atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg
    37081 attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc
    37141 atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta
    37201 gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc
    37261 atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct
    37321 tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt
    37381 ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc
    37441 catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt
    37501 atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt
    37561 ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg
    37621 ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg
    37681 tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc
    37741 cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac
    37801 tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc
    37861 tactgtatct acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac
    37921 tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc
    37981 tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt
    38041 tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat
    38101 cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa
    38161 aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa
    38221 tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat acacacccac
    38281 tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc
    38341 agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac
    38401 catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc
    38461 gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa
    38521 gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat
    38581 gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa aatcaccgac
    38641 caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc
    38701 catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa
    38761 aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa
    38821 atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa
    38881 tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc
    38941 actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga
    39001 tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt
    39061 caaaatattt tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt
    39121 atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt
    39181 gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac
    39241 tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa
    39301 atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac
    39361 atctgtgctg caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg
    39421 ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt
    39481 ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc
    39541 aattcaatta gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa
    39601 aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta
    39661 gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg
    39721 tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca
    39781 tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc
    39841 tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac
    39901 ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac
    39961 ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga
    40021 gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt
    40081 ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa
    40141 agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta
    40201 tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt
    40261 tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact
    40321 gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg
    40381 acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga
    40441 acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat
    40501 ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag
    40561 agattttcga ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca
    40621 aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga attggagata
    40681 tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca
    40741 ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt
    40801 atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc
    40861 cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac
    40921 ttattggaat agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag
    40981 attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga
    41041 ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc
    41101 agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac
    41161 cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt
    41221 agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc
    41281 cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc
    41341 ttttccagct tcaacaaaaa acctttgttg cgaattgtac ggatctccgg aagcttttta
    41401 ctgctcaacc agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg
    41461 aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt
    41521 atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc
    41581 gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc
    41641 aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta
    41701 ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa
    41761 gtaataagaa caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta
    41821 tcgcagatgc aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc
    41881 ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta
    41941 ttgccgatag gctatctctg gcacaccgtt tattcagaaa aaggagctgc gacaaggctt
    42001 ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat
    42061 cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact
    42121 gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac
    42181 agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta
    42241 cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca
    42301 gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag
    42361 tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg
    42421 ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct
    42481 tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta
    42541 atgcaagaaa tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat
    42601 aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa
    42661 atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag
    42721 cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat
    42781 aattgatttt tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat
    42841 agccttgcta aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg
    42901 tcttatttag tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc
    42961 tatcttcagc atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg
    43021 gttttcctga caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact
    43081 agcagcatga gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac
    43141 gcatgtgtca tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata
    43201 tgaatcatta tctagctcct tagctgcttg aattgcaagc ctgatatgcg gataccctaa
    43261 tatcgcttct tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt
    43321 ggttttttta tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag
    43381 tattttcgat aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa
    43441 tagccccatt gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat
    43501 tgccttttcc gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc
    43561 atgatgccat gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt
    43621 aagaaataaa gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc
    43681 aggttttgga cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg
    43741 ctgagtcact gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta
    43801 ttacttgctg aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc
    43861 aaatgcacaa cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg
    43921 cagccaacta tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata
    43981 cgtacgataa gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac
    44041 aatcattttg gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg
    44101 gatcgtattc aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga
    44161 acggacagaa aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc
    44221 caaatattga tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact
    44281 agcaacaaac cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt
    44341 ttgatagaac agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac
    44401 atatttttta ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg
    44461 gatctgcagc atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt
    44521 cagtaaaaag cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca
    44581 aagataagcc gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa
    44641 aaattcccaa taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat
    44701 aaacattggt gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt
    44761 tgcgctgtgg ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac
    44821 agctgatact ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc
    44881 tttctctctc tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt
    44941 ttcaatagaa tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc
    45001 cgagtcacta cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga
    45061 attggcaatt caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt
    45121 actgccggaa tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat
    45181 cctgataata tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc
    45241 tcttgtctat acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca
    45301 tgttttgaga tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt
    45361 agtatttctg cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga
    45421 acgacccatt caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca
    45481 tgtgcttgaa tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt
    45541 gtttcgacac gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg
    45601 tcatttgtat tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca
    45661 ttttgttctg aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt
    45721 tttgtataat tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt
    45781 tcagcaaccg aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta
    45841 atttctttat tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca
    45901 cctgaaatag catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt
    45961 gcataagcag cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag
    46021 ccgtatgctt ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct
    46081 ttatcttctt cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca
    46141 acttcaccgt ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg
    46201 atggcttctt ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt
    46261 ggatttttca tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt
    46321 tcatttcctt ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct
    46381 tcattgtaat cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa
    46441 taaaatgctg agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca
    46501 gcagctttga ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc
    46561 cgttgacgag atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga
    46621 acgcctgctt cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt
    46681 gcgattaaat aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt
    46741 cctggaatca aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt
    46801 tctgaaatag ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt
    46861 acaatgactg gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca
    46921 gcaacgattt ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata
    46981 gcgattacta ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca
    47041 ctttcaggga cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca
    47101 atcaattcac gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca
    47161 aaactatttt tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc
    47221 tggtgtagca ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa
    47281 tctcgttaat acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac
    47341 agtagattct gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac
    47401 tcctacatgg attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc
    47461 catggatctc tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt
    47521 cttccatctg ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg
    47581 aatttcattg gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt
    47641 gcagctaata atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc
    47701 acgatgccgg ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt
    47761 gtaacaccgc caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac
    47821 aaataagcaa ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact
    47881 ggtgcattgg ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag
    47941 actggttttt tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc
    48001 cctaatggat catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg
    48061 ccaggattat gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg
    48121 gtagaagagt ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca
    48181 tattgtcctg cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca
    48241 aaaaagacat ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta
    48301 tggatgcttt caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat
    48361 acccagttct tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac
    48421 aattcgatag ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct
    48481 gcgtagattc ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga
    48541 ggatctcgtg tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct
    48601 acccagtcac cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg
    48661 atcacgatac catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg
    48721 aaacctgtgt acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt
    48781 ggaatcaaat cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat
    48841 ttcattgtgc cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag
    48901 aaatcgatct tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata
    48961 ctagacatga tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct
    49021 gaaagaaaac ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca
    49081 atcagttttt ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag
    49141 ttgctgattt tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt
    49201 ggtttttcca taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca
    49261 tgttggtcta gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc
    49321 tttgtcaaag tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc
    49381 aagtccatca tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga
    49441 atctcttctg cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca
    49501 tcagatagat ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag
    49561 agacagtgag atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg
    49621 gcttctgcca acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga
    49681 caattttcat ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc
    49741 tcagcttcgc ttctcgtcac acctactact acgcccattg cataaccgct tggataagct
    49801 tctttcatta agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat
    49861 cgacaagcaa cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga
    49921 ataccgtttt tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg
    49981 ataaaaaccg tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt
    50041 tctaaacgtt ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct
    50101 tgtccaggaa ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc
    50161 actgctatct tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa
    50221 caaaaaggta tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa
    50281 agtattcgaa acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc
    50341 cttgagccgt gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag
    50401 cggcagaaac attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac
    50461 attagacaca ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac
    50521 ttccgtcata ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt
    50581 tatggaaaag catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat
    50641 taccgatcct gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat
    50701 catgaaagaa cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca
    50761 tctgcaatta gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt
    50821 ccgtcataca ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac
    50881 gacagatgat cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt
    50941 cccaaaagtt tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga
    51001 tttgcccttg acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat
    51061 caaaatgatt gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg
    51121 ggacaaaatt cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt
    51181 agaaaataag ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat
    51241 ttcttgaagc taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg
    51301 ttgatttaaa acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta
    51361 gatcaataga gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc
    51421 ctcgcaaaat tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat
    51481 ggtcacttgc atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt
    51541 ttttgctttt ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg
    51601 gagacaaatt gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct
    51661 tccatctctt tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga
    51721 atacaagtag ttgctaatca aatacttcga tatttttctt ttttatcaac tcttaaatgt
    51781 tcaatccgcc tgtgttggtc ttgctaccat ctactattaa aatgtttatt attgtttgcc
    51841 cacctatttt ttgattttag tggtatcatt tttcaagttc atgatgaaat tcttatcatc
    51901 agtaaatgct ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt gaccaccgtt
    51961 tattttctcc gtattcttag aatagaaggg agtctgattg atgtattcac taatgaaaaa
    52021 aatcattacc gaaaaagata ttcgacgtca tgtttcttta gtagaacaat tgctgaatca
    52081 tacaaagata accgtaaatg aattagctga aatcataggg acaaccgaac gaacgatttt
    52141 ttctgacctt caatctattc gctctcagct gcccgaaggc tgggacatca tcagcgatca
    52201 agcaggaatc agtttgcaaa accagcaaaa tttattaaca aatgaccttt gggagatttt
    52261 tttcaaacaa tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa aaaaagttgc
    52321 tgtccctgat tttctagccg accatgggct ttcttatggg acattgaaaa gacatgtgac
    52381 taaaatcaat cagcgtttag caagttacga cttgcagatc gatttgacga aatatacagc
    52441 ttgtattctt ggaaaagagc gtgcgattcg tactttttat catcgattgc tgattccttt
    52501 cactcataac aactattttt tcgaggatta ttcgatccat gaatcccatt attttcaatt
    52561 tttaagaaac ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt tcggaacatg
    52621 ttggttcttt atcaatacga tccgtataaa agcgaattgc cgattagatt cttctatcca
    52681 taccaacagc acactctcct ccctgtatga ctctgcattg aaaaaattgt atttaacgga
    52741 aggtatttat cttaaagaca ctgagctttc ttttgcttct ttctgctttc tggaaagttg
    52801 gaattacaac aataactatg gacaagaaac cgctcgctgc ctgcatcatt ctccattttt
    52861 agaggtcctc gaaacgtttg tagaggaact agcttcagag ctgtctcttg atcaactaaa
    52921 aaaaacatct ctgactgata acttggctct cctcatatta aaatatcatg aatcgccgat
    52981 attgattgaa gcattggacc gtcaatacca tcgtttttta gaaacatatg atcagcaata
    53041 tgcacacttg tatcaacaga aagagaattt actgaacgat ctaaaaaaag aaatagaaat
    53101 caatgaacca aattactttc tccaactctt gtctctactg atccaacaaa cgattttttc
    53161 tatcaaaccg aatttgttca atgtctattt tattttccaa ggagaacctt catggaaggc
    53221 gttcttgcag caggaattaa aagattactt aggaaaacgt gtctgcttct tacctatcga
    53281 actaaccgat ctttccacta tttcttttga aaaaacagat atcttgattt ccaatttccc
    53341 tctagattct ctagatatcc ctattgtata catttcttct attccaacga aaaatgagct
    53401 caatcgtttg actgaactca ctttcaaaac ttttttatag taacggctag cgactgtatt
    53461 ctctagctgt tttttctttt ttgcgaaaca gtttgttcat gatccctttt tctatttcta
    53521 caactaagaa tagaagaatc acatttgctc caatcaataa ctgttgtgtc atcgttaaac
    53581 ttactgtacc gatcagctgc tggccgactg gtagaaaaat aaccatccct tgcaataaag
    53641 caaggatacc taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg ttcaatgacg
    53701 gcttggacaa ctctctgcaa ttgatcatgt aaacagcttg agctaagacg atattttgca
    53761 gaagaatggt ttgctgtaag ctttgccctt caaactgcat agccaataaa taggcaggaa
    53821 ccatgatcag tagggagaca tagatgatcc gaaacaaatg ataaccagat aggattcctt
    53881 gctttggatc tcttggtggg cgcttcatga tttctggatc tggcgactcg aaccctagtg
    53941 cataagataa agtgattgtc gttaccatat tgacccataa aatctgtact ggggacaaag
    54001 gtagcgggcg attcagcata agtgcccaaa tcaagatcaa tccttgagct aatgcagttg
    54061 gcaagaagaa attgatggtt ttttgcaaat tatccaaaat ccgccggccc tcttttacgg
    54121 cttttgcaat cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc tgtttgctta
    54181 cttcactacc tttgattccc atagcaattc ctacatcgga tcttttcaaa gcaggtgcat
    54241 catttacgcc gtctccggtc attccgacga tttctccatt tttctgcaaa gctttaacaa
    54301 ttcgcagttt atgttctggt gtcgttcgag caaagacatc gactattggc acttgctgaa
    54361 tcaactcttc atcagacata agatccaaat cgatcccttc taaaactttg gcagtatgtt
    54421 ttaagccaat ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt
    54481 tcactgagat ccctgcttgt tggcattcct ttacagcttt gactgcactt tcttttggcg
    54541 gatcgatcaa tccggcgatc cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt
    54601 cctgtttcga atctactttt ttataagcaa atcctaaaac acgctgacct tttctcgcaa
    54661 attctgcagc tgtctgactc catgcttctt gttctgcaga agaaagagtt gatttagcaa
    54721 ataatacttc tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg
    54781 ttgccatata tttgtattca gaactgaacg gaatctttgc ttctattttt ttcaaaggta
    54841 gattggctgt ttctgtgtgt cgaatcagcg caacttctgt cggattgcca cttaaatttt
    54901 ctgttttctg ttcttctttt gtttttaatt cctgacagtt tttcataatg gacaacacgc
    54961 aggcttcttc ttttgcagct acatccacaa ctgtcatttc attttttgtc aatgttcctg
    55021 tcttatctga acagatcact gtcatagaac ctaatgtttc taccgaagga agtcctttga
    55081 tgatcgcctt ttcttttgcc atttcatgta ctccaacgga tagaatcatg gtcaaaacag
    55141 ctggcaatcc ttcgggtacc attgacacca ccaaagcaat cgtagccgaa agaaggatat
    55201 gccaatccat accatatcga aatgtcgtaa aaataactaa gaataggctc aaacaaagaa
    55261 tgccttgaaa aatttgtttg tttaatcggt tgattttttt aatcagaggc gtcgtttgtt
    55321 gttcgaccga ttgcaatgct tgattgattt taccgatttc tgtcgtgttt cctgtttcta
    55381 caacgacacc gactgcagaa ccagattgta caagcgttcc tgaaaaaccc atatttttct
    55441 ggtcgccgat cgattcttct tctgtcaaaa cggcatgact tttttcagat gcttctgatt
    55501 ctcccgtcaa aatcgattct tccactaata aatcatgtgc ttcgattaat cggatatctg
    55561 ctggaacgac atctccggca cgtaagacaa tgacatctcc ttttaccagt tctttcgcag
    55621 ggattttttc ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc agctgcttca
    55681 aaccattcaa tgattcttcc gcttttcttt cttgccagta actaacgagt ccattgacga
    55741 tcactacgaa taaaataata cttccttcaa tatattcact agaagcaaat ttcaaaaaag
    55801 aggcaatgat caacacgatc atcagcagat cagtaaaatg ctttgctatt tttttccatc
    55861 gttttgtctg ctgccttgct tttatttcat tatatccttg atcagtcagg cgtttctgtc
    55921 gttcttcttg cgacaagcct tctgttgatg tttgatagtt tttcaataca tcttctaccg
    55981 tcaatctgta ccagttcatc ctatcccctc ctgtttaatt gatactgtta ttatacgcag
    56041 ggaagttttt tcaggcgtat cgattatttc aggggggtct gaaataattc gttttgcaaa
    56101 atgtatctgg gcctaactga tatttctatt gcttattctt ttatatacaa aatttaagaa
    56161 tacaaaccag ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct
    56221 gaaagcaatt gaagtaaaaa acaccaacaa agcaagatct ttatatcttt gtgtagtgtt
    56281 ccgtttcttt tctttaataa attgaacatg tgcacacaag atcgaagacc acacaaatag
    56341 aaaacaactc gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa
    56401 aaaatgtaga caaagaccgg caaaaaataa cattcctgta attaaaatac cgtgtaaagg
    56461 tacgcctttc ttagaaagcg cacaaaacca atggggcaaa cttttttctt ttgctgtttg
    56521 atataaaatc ctgctagtac tgtagactgc actatttgct gaggacaaag aagcaaccaa
    56581 aatcattaga ttgacaattg ctgctccgcc ttggcaatgg atcacttgca gcatagtcac
    56641 taaaggactt tcttttaaat ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat
    56701 cgttcctata tagaaaaaga gaatacgcca aggcagacag ttgattgctt ttttcaaatt
    56761 cttttgcgga tcacttgtct cgccaacggt taagccgatc atttcaattc cagcaaaact
    56821 gaataaaacc ataggaaatg agactaccat tccagcaaag ccaaacggta gtaattcatc
    56881 gatctgtcca aaattttctt tgattccgat agactgggta tgaaaatctg agacactcaa
    56941 tcctataccc aataagataa aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga
    57001 ttccaattca ccaaaccact tgactgcaac aaggttgact gccattaaga tcattagtgc
    57061 gctacctccg cttatcaaag ctggtacatg tacaaaccag aaatggatat acaatcctat
    57121 tgctgttaat tccgcaagtc cagctccaag ccagcaaagc caataagtcc aattgacaaa
    57181 aaatgcccac tttttgccta aataatagtc aacgaaatca ctgatagagt taaatgtact
    57241 atttgctaaa aataaatcac ccatggcccg catcaaacca taaatagcca aaccgcatat
    57301 caaataaacg agaataacag ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa
    57361 taatcctgtt ccaattgcgc cacctaatgc gattaactgg atatttcgat tagttagagt
    57421 ccgtttcatt gccggttttt ccatcaagtt cacctttttc tttttcaagc agcatggcga
    57481 cttcctgttc ccaagaaggt ttctttattt tttcaataca gagtggtact acaaacaaaa
    57541 tgacactgat ggctgtgact gcaattagat tgatccatgt tccagctttc atcgtcagtc
    57601 cgacagaaaa tccgattgcc gcaaataaaa caataacgac agtccaaact ccaagattgc
    57661 tctttttctc tttttttcca attctaaatg gtcgattcaa ctttgcatgt gtataacgca
    57721 atctcaaaac tgcgatcccc atgatgacat agacgataca gtagataaca gtagttgaat
    57781 ttacgagcat caaaaatgct tgattgacgc caggaatcag caaataaaaa agtgcaaaga
    57841 ttgagataat gacagactga gtcaaaataa cggatttgga tacgtcaaat tgattcgtct
    57901 tccaaaaatt gaattttggt ggatacgcac cttttctagc actttctgtc actgtctttg
    57961 ctggtcctga ggcccaagca gataattgga ctgctacacc aatgaagaca agtaaactaa
    58021 acagattcac aataagatgt ggcaaaccaa gaatttggca ataaatcgaa atagattgcg
    58081 cgatattatt caattccatt tgtccttttg gtacgacatt tgctacaact aaagcattca
    58141 aaatattgac gaaaaatata aaaattaacg cggcaaatac tccgcgaata tacgttttaa
    58201 ctggattttc caatcgagtg atatacacag aggacatttc gataccagta aaaataaaca
    58261 tgatcggcgc aaaataaaca aatgaggatg cagtcgtagt atctggtatt aatttgtccc
    58321 atgaaaaact gcctaatgtt ccggttggta cgatcccaac tttgatccac gcagcaaacc
    58381 ctaataagag catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg acttttccgc
    58441 cgatttttgc catatcaaat ttcaaattca aaaaagtgat gatccaataa acgactaaaa
    58501 tgacaactaa tgtaaatttg ctgttcaaac caagcggcac attatcgata atattaccaa
    58561 acaacggagc gagtgctgaa gcgaccatca ccatcccagg aaacatttgg acccataaca
    58621 gccatgagac aacgaacccc catttttttc ctagagcatt cgatacccaa agttctggac
    58681 cacctttttg gggaaacatt ccagcaaatt caccagagat caatgagatt ggaaaagcgt
    58741 acaataatac tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg
    58801 gaatatttcg tacacttgct acaagggcgc aggtcattcc gataaaggtc atggtcgtga
    58861 tttgctttga tgaaccagta tctttcatct ttattccttc tttcaatagc gacatatgtt
    58921 tttgttttct tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca
    58981 aaacccagta tcaccgaaac atccattgaa cgatcttagt gaactgggtt tgatacatga
    59041 aacgaacgat ccaacttata gaacgtttta taaaaaagaa tatcttggcg aggaatatat
    59101 gaaaaacagg gggaaataca attgttttga tgaagcaaat aattgtctgt tctccatatg
    59161 tctcttgtta ttctttcaca caaaaatcag ttctattgga tgatttcgtt tagtttcttt
    59221 tccattgctt tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc attcaaataa
    59281 ggagtcatga tagccgcacg tagcagagtg attttctgtt cttttttcca ttcttctttc
    59341 gttagaccca ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat
    59401 gtagtatgag aagtaataaa ccgttcacca taaacatcgc catccatata agaagaccga
    59461 tcgaacattt tttcatttaa ttcattgata gcgttcaaat ctgtacagcc ttgttctttg
    59521 aatacccaat tgaccatatt aaaatcagga tgattcaatg gatagacttc gattgttttt
    59581 cctttaacag taaaagtcag atgatcgaga aattctctga atctttgagc tgcctcgatt
    59641 gaagccccaa tcaattgacc atatccggtc acattcaatg gcaatacacg atgtgctgtc
    59701 cagactgcgg cggcggttgc tccagctttt gatccttcca aaatataagc tcctaacata
    59761 tctggagcct ttactgattt ttcaaacaca tatggcgcga aataagaaat gatatttctc
    59821 atatttttat gtttgattac gattcctcca gcagcataag ggacatagcc catcttatga
    59881 ggatctatcg tgacagaatc cgcttctgaa atagctctga acccttcata tacctctttg
    59941 tcaatcgtta cgcagtggtg gaaaacatga tgttcttcaa agaattcagc taaggaagca
    60001 tacggcacaa attctcctgc ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg
    60061 gcatcaacat gtaaatagaa atagatccct tcatctttta acctttctct taattggacg
    60121 attttatcta cactgtctac ttgaccttct tctgtcgttc ctacgacagc aaccacacct
    60181 agaattggga ttttttgtcc agctaattca cgaattgttt tttccaaagc attaatatcc
    60241 attcgataat cctcttgaac tgggatagca accatctgat ctaagccgac tccgcaaata
    60301 tctaacgcct tcatccaaga atagtgtttt gtttgaggga ccagccattt ccctaatctt
    60361 tggatatttt ttccgctacg ggaagaagct gctttgacct catccatttc ttcgtccgta
    60421 aagctttcgg tcatttccaa aatttcttca acagataaat tcaaaagttc ccactcagac
    60481 atttttttca ctttttcagg cagcacttcc ttgacagcta aaggaatcga tttgatgcaa
    60541 cgggcgtacc ataatccttc aagattagca atagaaccat ctgctgcaat gtgtccccat
    60601 ccgtctgtca tattgaataa tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa
    60661 gttgccatag aagattccaa tgcaacatta tttgggttcc acagcatcgc atagttgtaa
    60721 gctaataaag caggcatcaa tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa
    60781 tgccatggga tcgatcctgc gcgtaatcgc tgactgactt catccaagac ctctatcata
    60841 tggtcgcggg tggctagata atctggagtg agcttatctc cttcgctaat tgcaggcata
    60901 tctgaaggaa tataattttc tctccatcca agatgttcat ctaccatctt gttcaacaac
    60961 attttataaa ctggtccatt ttcagctttg tcgcctataa agacggcctt gatatccata
    61021 tctttcattt ttcttccctc catattttgt tggtcaatgt cttgcagata catgtgttta
    61081 tctgtaacac ctcttcacta tagcgccaaa aatgagaatt ttttttttat aacatcaaat
    61141 ccacgtagat ttttacaaac aaaaataaat aaagtcggat ttttctgata attcaacgca
    61201 aaaaagcgaa caatttttca ataaatcagt cttctaaaat agataaccta aaaaaagcga
    61261 acaaacaaat aaaatgactg tccttcattc actaaacata tttttatttt tttatattgt
    61321 taaaaattcg caatttattt tttaatagaa ttatctgttt tccaaatttt ataacataaa
    61381 aaaccttttt taacggcatt tatatgcttt ttcagaaatt aactgatgat ttcaaaaaga
    61441 aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg ctctactatg
    61501 attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg gcaattctat
    61561 gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg caaaaccact
    61621 ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc ttatacacat
    61681 cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg caagatcatg
    61741 cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt ggaagaagat
    61801 gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa agcttattca
    61861 ttaaaaaaac aagtattagc ttattttaat gactgcggga tcgaaatcga ccgctactcc
    61921 cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta tcgtttaggc
    61981 ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg tttcattgaa
    62041 tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat cttatcgatt
    62101 ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga ttcaaaattt
    62161 atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg ggaaaacact
    62221 gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact attcaatctt
    62281 tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca gctacaccaa
    62341 gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga atctcacttc
    62401 aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct gatgcgttcg
    62461 gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa tgaggaacag
    62521 acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca gttaccttat
    62581 gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag cggaattctc
    62641 cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt tcattatctg
    62701 atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc accaacaatt
    62761 tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg ggtattatgt
    62821 gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc tatttcgatc
    62881 aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc tgtgacaaaa
    62941 atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc tcttgataca
    63001 cgtcaggctc acaacctcac ttattttgga catctacggg tccgatctct ttatttaaaa
    63061 aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata tcctgcaagg
    63121 taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa cttagaagta
    63181 tatagcctcc caacatcttt ttaatagcat tgttctcgct gactcggtcg ctttgcactt
    63241 ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca ccccaaacca
    63301 ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat ggaagaagaa
    63361 actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt tgtcttctat
    63421 ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg caagcttcaa
    63481 aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga ctagtacttc
    63541 caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac gagtattacg
    63601 ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa caagaacgat
    63661 cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc attctagaaa
    63721 ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt gttcctattc
    63781 ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta atgataacgg
    63841 ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat aaatcgtgga
    63901 agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag cttttcaaag
    63961 aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc atacacctcc
    64021 tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga cacttcttct
    64081 tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa taaaaacgaa
    64141 ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc
    64201 aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg aaaaaagaca
    64261 ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta gcatcctatt
    64321 tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg agaaaaagta
    64381 aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat ttgattcaag
    64441 aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac ggttcaaatg
    64501 atggtgcacc gaccgacact gtagtggatt aatattggaa taaaaaccat ccattagaaa
    64561 accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt
    64621 tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact gctgaagaag
    64681 atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg atggaaggtg
    64741 gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt attcttctga
    64801 ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc actatcgaag
    64861 aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg tgggaaacaa
    64921 catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg catcaaatga
    64981 tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt tacaatcaat
    65041 acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc aaaatcattt
    65101 tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat gaaatcactg
    65161 tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt gtggtcgatg
    65221 aagatacagc agttattttc actaatgggt ccattacaga ttcagctact ttaggagatt
    65281 acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc tggaaaaaag
    65341 ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat cgagatgcaa
    65401 gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat gaaatcaccc
    65461 gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc acaccgatca
    65521 cgccgttagg ccaaaaagat gtcaatatgt ctatcgtcgt gcatcatcaa ccgcatttta
    65581 cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca agacgacgtg
    65641 gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta gaagaactga
    65701 ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa gaaacagaaa
    65761 ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc gcgttgttca
    65821 ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc aatctggcct
    65881 ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa agtgctgtac
    65941 gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt cttgtcaaaa
    66001 caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa atgtttgaat
    66061 aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc aggctaaaac
    66121 agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct tttgaacacc
    66181 gtttaccaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca cccaagctaa
    66241 aactgcttct tatgtttttg acttggttgg acttattacc gcagatgcaa gcttttgaac
    66301 accatttacc aagaggttat gaaaaagctg ccctgcatca agtaataaga gcagccaagc
    66361 taaaacagct tcttatgttt ttgacttggt tagacttatt accgcagatg caagcttttg
    66421 aacaccgttt accaagaggt tatgaaaaag gcgtttagtt tcgagtatga agaaaaattt
    66481 tgtgaaaatg gttcctcacg tttttgcata aatttgactt aataccgaga aactgatccc
    66541 ttctgtcata acctcttttt atttatgtct gtttcttttt gaaaacaact attttttcaa
    66601 cgtttactct cttgccaatc caacatcatc taacggatac aactatttcg atgattacca
    66661 ttacactaaa aaatcggaaa cacacctctc cgaacacaaa tacataagac tatagaatgt
    66721 ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt aaaatgaaat
    66781 tatctgtagt actaataatg cagacaattt tatgaatctg aaaggagcat ctccaatgaa
    66841 agcagtcact tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg atccaaaaat
    66901 attagaacca acagatgcaa tcatccgtat cacagcaaca gccatttgcg gctccgatct
    66961 gcatctttac catcatggtg aagcagtttt ggaaaaaggg tatgttgtag gacacgaacc
    67021 aatgggaatc gttgaagaag tgggatctca agttcaaaca ttgaaaaaag ggcaacgtgt
    67081 ggttatccct tttaatatta gttgtggtca ttgtcatttc tgcctgaatc acatggaaag
    67141 ccaatgtgat gaatcaaatc cagatcagtt atatggcggg cttttcggat ttggcaagtt
    67201 gaacggtaat cattggggtg gtcaagcgga atatctaaga gttccttatg cagactctac
    67261 ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa gttttatttc tatcagatat
    67321 tttgcccaca gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg atacagttgt
    67381 gattctaggt tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta tggctggagc
    67441 agagcgcgta atcgcagtag atccagtaca gcaccgtcta gataaagcgg ctaattacaa
    67501 tggggttgaa acttatttct tagaagacac ggcacaagct ggaacagaat tgtatgaact
    67561 ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga atggacggat tagagcctgt
    67621 caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc acaatctctc cattacagat
    67681 ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta actggtgttt atatgactcc
    67741 agcttcttct tacccgctgc aagaattttt tatgcgggat atcgctgtca agcatggaca
    67801 ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg attgcagaag gactattcga
    67861 tcctacacaa atcattactc attcgctgcc actatcagaa gcagcacaag catatgagat
    67921 ctttgacaaa aaagaagata aaaatataaa agttgttttg aaaccttgaa ttcagtaaaa
    67981 aatacaggtg attttcttta tgcccaaaat caaacgaaat acccatgaga tcgaaatagg
    68041 aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc aaaatcgtta
    68101 cacattgatc tcactggctg tcgatttatt aaccgggatt ttctatattt ttggaagcct
    68161 tgcttcctta actaaaatcc ccgatcgata tggcacctat ttttatttgg ctggtgctat
    68221 tttcttgacg attcgtccta ttctcagaat cgtaaggaac atttttattt acgacgaaaa
    68281 aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga gaatttttca aatctcatgc
    68341 ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc actttcttcc
    68401 ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg atctgtaatt
    68461 aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc taatatccgc
    68521 ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag atttggtgtc
    68581 agatcagggc ggttatggaa gtcgttttca atgacgcccg ctcctccgac gatggtttct
    68641 ttttcatcta agaccacata ccattgagga atactttcct tattagcgat tccagtacga
    68701 atactttcct catacacaac agcaggaact ccccattttg atgaaaacca attcgcgctt
    68761 tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac caatgtttaa
    68821 acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa aattagcaat
    68881 gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc cgttttttga
    68941 ttgatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc ttgacgaacg
    69001 gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca cctgcaatac
    69061 tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc ataacggtag
    69121 ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag accattggtg
    69181 tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca aacatcgttg
    69241 gttcattaat attgaaggac acctggcgca atcgacaagt ttgttacaac catatatggt
    69301 ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc acctgttcct
    69361 cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc gctatgccca
    69421 gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg catcaaagga
    69481 tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat aaatgcaagc
    69541 agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac cactgtatag
    69601 aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac
    69661 gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac tgccggtggc
    69721 acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac aaagatttct
    69781 gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat acctaatgct
    69841 ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc gtcttttcgg
    69901 tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag aatggcaaat
    69961 gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa aattgagttc
    70021 atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa tgctcctaag
    70081 atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg ccgttgtgca
    70141 ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa tttatccata
    70201 aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca gatcaatgat
    70261 ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac tgtggaccgt
    70321 caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct ctgtttgaga
    70381 gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg ctaaagtgaa
    70441 atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat ttccgccaaa
    70501 catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt gtatcctccg
    70561 ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt tcaccattca
    70621 tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt gctagctttt
    70681 cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg tctacatttt
    70741 tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct gcttccattc
    70801 cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc attccagctg
    70861 aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct atacttacac
    70921 ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg atatcatcac
    70981 atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac taaacacaca
    71041 aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact
    71101 tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt aataagtcca
    71161 accaagttaa aaacataaga tgttgttttt actttgctat tcttattact tgatgcagga
    71221 cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg gtattagctc
    71281 aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa tgaacatcta
    71341 caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct cttatcaatc
    71401 aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc gttcaattcg
    71461 attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca gtcagtgaat
    71521 gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat caagtaatgc
    71581 aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt ctcatccata
    71641 tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg gatattatga
    71701 taattttctt ttagatacat ggctatatca tacatgattt tcggatagat ttcccatccc
    71761 ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg ttcaggatgg
    71821 aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg ataatattga
    71881 ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga
    71941 tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta gtatcctttt
    72001 accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc agctttttgg
    72061 tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca gccaatcctt
    72121 ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa aactttatga
    72181 taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa
    72241 tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt taccctatca
    72301 ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac ttttcttgaa
    72361 gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc caaacaaatc
    72421 atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg ctgagcatat
    72481 tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt gatagaagtc
    72541 cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta ttgttccata
    72601 aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc atttttatac
    72661 cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc cgtttgccaa
    72721 gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat gatttcctcc
    72781 tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg gcagatagat
    72841 caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat ttcctgctgt
    72901 tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa ctgctttcgc
    72961 cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag gcgttaagat
    73021 aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa ctggttcatt
    73081 gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat cttcccgttt
    73141 tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc ccatactcat
    73201 atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag cttcaaaagc
    73261 ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct tcaccatttg
    73321 atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata gcaaaatacc
    73381 cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa tgatatcata
    73441 aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac ccgatacagt
    73501 cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca cggtatcagg
    73561 cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa tagaaaggat
    73621 ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag ccaatacgtt
    73681 ttctactata acagtctgat tttcattcac taattcataa acagttggat taacagataa
    73741 ataagacatc actgcgatga taccagggaa tacaccatcc actttattca gcttggctaa
    73801 ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga catagctgat
    73861 ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct gtgctaagcc
    73921 attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg taataattac
    73981 cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat tattgattct
    74041 cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc cagatcccct
    74101 cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc gcttacattt
    74161 cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa attcatctga
    74221 tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc tcctattatt
    74281 ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa cgcagcaagt
    74341 gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc
    74401 agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat gattgggata
    74461 atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa atcgatgtct
    74521 aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg cgttaatttt
    74581 ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc catcttaggc
    74641 tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa
    74701 gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt ccccgctcct
    74761 tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg caccgtgctt
    74821 cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc tcctacttgc
    74881 aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac tagggtttta
    74941 ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa ttattataac
    75001 acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt catagacata
    75061 caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc tagccttcga
    75121 tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc ttcattcgat
    75181 tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt acatcgtttc
    75241 tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa attatccatc
    75301 attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac acttgtaaca
    75361 gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg
    75421 gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc ttctgaaagg
    75481 aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag ataacgattt
    75541 gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc ttcttctaat
    75601 actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg catctgatga
    75661 ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt atcttctcca
    75721 ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg catcaaagct
    75781 tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca
    75841 taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga tttacgagag
    75901 ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga aataatcagt
    75961 tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt ctctgcagct
    76021 ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat gtcgaatgcg
    76081 cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt ttgccataat
    76141 ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata ttccattttt
    76201 tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat ctttttaaaa
    76261 cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt aatcaaaatg
    76321 attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca gaaattgatt
    76381 acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt gctgatgata
    76441 gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg gttggtggaa
    76501 agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt gtcgttcgcc
    76561 ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc caaatgtatc
    76621 tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta tttctcttgt
    76681 gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta cttgatcata
    76741 atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga tccctttttt
    76801 cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg cacgttcttg
    76861 atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct tatcaaatcc
    76921 ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc ggatagctaa
    76981 tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat tttctcttat
    77041 gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg gattcaaaat
    77101 cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc ctcctttccg
    77161 aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa taagcgatta
    77221 cttcatcccc ctacagattt ttcgtataaa aaagagagaa tccttatcga cagattctcc
    77281 tttttaatca atttatttta taaatgaaaa gttaatttac cttttatgga aaactatttt
    77341 aaaaaataaa cataaatatg cagttaatgt aacaagataa gaaataatag tcgttactgt
    77401 ataacttgtg cttactttaa tatcagcaac tcctttgact ccgcttattt gtaccaatcc
    77461 attagcattc acctgctcct ttacaggttg atgatttatt tctacattcg ttcctaaata
    77521 agcatataca ggaagtgtga ttacttggct cttctcatta gttactctat atatgataga
    77581 actaccagtt gttttataag gaaggtttac ccattgactg ccaatctttg cttcgtgttt
    77641 tattatatgc tccttattct ccaatgaagc gataggagaa taatcatata aaaaataatc
    77701 cattccttta gtggtatacg cctctactgt ttctttgttt acaacaatat gtcccggctg
    77761 agaaattatt ttttgattga aatttttttt actagaaaca gttaaagtga ttaataacaa
    77821 aacagtcata aataaataaa atagttctct tgtcttattg tatcttctat ttttgaaaaa
    77881 tatcatcgtt aatatcagag aagagaatat tatttgcaag cttaagatcc gccaaggaaa
    77941 ttgtaacaat ttggctggcg tattttgcag aagataccat ggcaccaaac tagttgtagc
    78001 tagccaagtc aaacacgtac cataataaat ctttttttct atgtttgtta attttttaaa
    78061 taaaatcagg ggaatacata aagataacag accaactagt cccattgagt aagtagttaa
    78121 gtcgttgttc atagcttcag tcagactatc gaacacatta gatgcatgag catataaatt
    78181 tgtcacgcta ggtttgttga ttttttggta gagcatttgt tcaaacatcg gataccaaaa
    78241 agcactcgtc agaaccaatg tcattcctgc cgcactaaat aattttttaa atatagcaat
    78301 aaaatctgca ttcttgaaac ctttagaaac caaacgaata aaaatgataa ttaatgctgt
    78361 actcattaat gttgatagta catggctgta cactagcaaa ctcatactca atgctagctt
    78421 tacagacgga tacttttctt cttttccaga gatgatttga taagcataaa ataatacagg
    78481 gataacaagt gttattgcga tagcttcggc aattgcggaa cgatagtaga tgtctactgt
    78541 ccgataagca gagaaaatat agagacaact aaataaaaat gccgcttttt tttgttttaa
    78601 aaaccgctcg ccataatgat aagctacgag tgttgtacac aagttcaata accaaacgta
    78661 aataatatag cttacaatca aattttttgt catccaatat aacattacag ctggaaaaaa
    78721 agtcagaaag ggataaaaat aatttacacc atatccagta taattgaatg tctcataatt
    78781 aatcggactg gtaaaaatag tcgataaccc cttgattcga gataagtgaa aagaaagatc
    78841 tccagtggta taaatactct gtgtattttt gaaaaagatg aaagaatata tactactaac
    78901 tataattaat ccactcaata ttactacttt tttatttatt ttcattcttc ttgtttatct
    78961 cctttttagt cacatatcga gaatctttca acttattagg tgcaaatcgt ttaaaacttg
    79021 aatacaccac taaacaataa gtcagaatac tgatccataa agccatgaga gcagttggag
    79081 tatattttgt tgtaatttct atcttatttt ctccagcatt tacatccaca ttcactaagc
    79141 catgtttttt ataagagtca tttacgactt taccgtttac ttttacctgt gtacctaaat
    79201 aagcatatac tggtactgtc actttttgct ttttgttgct tgccactgtg taagtaatgt
    79261 tattatctgc agctttatac ttcccttttt cccacgattc tcctactttt acttcatgtt
    79321 tttctaagtg aggcctttct tttatcgctt ctgttggggc gtaatcgtat aatcctccca
    79381 tatctgatgt tgtataacgt gggagtgttt cttcattgat aacaatatga ccgtgttttg
    79441 attggataac ttttgcgtaa ttttcttttg ctgctataga cgtgacgagt agaaacacca
    79501 cagtagcgcc aatgattaac catgaatatt ttttattcat agtctttttc ataaaaacaa
    79561 gcgttatgat cattgaagaa aaaaagattt ggatacctaa gatacgccaa ggaaactgga
    79621 gcaagcttat cggtgttttt tgaaacaaaa accatggaaa taaattagtt acgcataccc
    79681 atgtagaaac tacgccaaca tagatgattt tttctttttt ctccatttct ttcagtatga
    79741 ataaaggaaa aatcaacgca accaatccta ataaccccat agtgtatgtt gtaatatcgt
    79801 tatttaacgc ttgaatgata ctatctgaaa tagaaagtgc ttctagctgc aaatctgttt
    79861 tgaagggact gtgtatttct tgataattca tctgctgcaa cataggatac caaaaatagc
    79921 tagttaatgc acaagtcaaa aaaacagcct ttactaaatt cacgaccaca aattttatat
    79981 cgttttttat ttgcttgctt aaaaacaaac gaactataat catacaagca atcaatgcac
    80041 tacacaatag tgtagttaag acatgagtat acacaagtag acttatactg acagataaag
    80101 cgatgacttt tttttctcta ttgaatatca atccataagc ataatataaa acagctggga
    80161 taatcgtaag agcgattgct tctgcaattg cggatcgata atagatatca actgttcgat
    80221 aagttgcaaa agtatataaa caactaaata aaaaagcagc atctatcttt ttaaaaaact
    80281 tttctccata ataaaaac
//
LOCUS       DACBZN010000004        64187 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.004, whole genome shotgun sequence.
ACCESSION   DACBZN010000004 DACBZN010000000
VERSION     DACBZN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.004"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(137..1528)
                     /locus_tag="IVD64_000252"
     CDS             complement(137..1528)
                     /locus_tag="IVD64_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP6147530.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IVD64_000253"
     CDS             complement(1741..2127)
                     /locus_tag="IVD64_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP6147531.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="IVD64_000254"
     CDS             complement(2183..2806)
                     /locus_tag="IVD64_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP6147532.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="IVD64_000255"
     CDS             complement(2870..3538)
                     /locus_tag="IVD64_000255"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6147533.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="IVD64_000256"
     CDS             complement(3550..3990)
                     /locus_tag="IVD64_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147534.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="IVD64_000257"
                     /pseudo
     CDS             complement(4049..4720)
                     /locus_tag="IVD64_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4905..5303)
                     /locus_tag="IVD64_000258"
     CDS             complement(4905..5303)
                     /locus_tag="IVD64_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP6147535.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="IVD64_000259"
     CDS             complement(5300..6073)
                     /locus_tag="IVD64_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6147536.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="IVD64_000260"
     CDS             complement(6070..6753)
                     /locus_tag="IVD64_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6147537.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7807)
                     /locus_tag="IVD64_000261"
     CDS             complement(6920..7807)
                     /locus_tag="IVD64_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6147538.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7804..8673)
                     /locus_tag="IVD64_000262"
     CDS             complement(7804..8673)
                     /locus_tag="IVD64_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6147539.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8810..10096)
                     /locus_tag="IVD64_000263"
     CDS             complement(8810..10096)
                     /locus_tag="IVD64_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6147540.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10369..10947
                     /locus_tag="IVD64_000264"
     CDS             10369..10947
                     /locus_tag="IVD64_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6147541.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10947..11876
                     /locus_tag="IVD64_000265"
     CDS             10947..11876
                     /locus_tag="IVD64_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6147542.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11873..13609
                     /locus_tag="IVD64_000266"
     CDS             11873..13609
                     /locus_tag="IVD64_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6147543.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="IVD64_000267"
     CDS             complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="IVD64_000267"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP6147544.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14505..15014)
                     /locus_tag="IVD64_000268"
     CDS             complement(14505..15014)
                     /locus_tag="IVD64_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP6147545.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="IVD64_000269"
     CDS             complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="IVD64_000269"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6147546.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16315..17406)
                     /locus_tag="IVD64_000270"
     CDS             complement(16315..17406)
                     /locus_tag="IVD64_000270"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP6147547.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="IVD64_000271"
     CDS             complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="IVD64_000271"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP6147548.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="IVD64_000272"
     CDS             complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="IVD64_000272"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP6147549.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="IVD64_000273"
     CDS             complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="IVD64_000273"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP6147550.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21369..22343)
                     /locus_tag="IVD64_000274"
     CDS             complement(21369..22343)
                     /locus_tag="IVD64_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP6147551.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22324..24207)
                     /locus_tag="IVD64_000275"
     CDS             complement(22324..24207)
                     /locus_tag="IVD64_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP6147552.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24225..26450)
                     /locus_tag="IVD64_000276"
     CDS             complement(24225..26450)
                     /locus_tag="IVD64_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP6147553.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26565..27599
                     /locus_tag="IVD64_000277"
     CDS             26565..27599
                     /locus_tag="IVD64_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6147554.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27666..28679)
                     /locus_tag="IVD64_000278"
     CDS             complement(27666..28679)
                     /locus_tag="IVD64_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6147555.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28757..28945)
                     /locus_tag="IVD64_000279"
     CDS             complement(28757..28945)
                     /locus_tag="IVD64_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP6147556.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29385..29513)
                     /locus_tag="IVD64_000280"
     CDS             complement(29385..29513)
                     /locus_tag="IVD64_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6147557.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="IVD64_000281"
     CDS             complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="IVD64_000281"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6147558.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31000..32055)
                     /locus_tag="IVD64_000282"
     CDS             complement(31000..32055)
                     /locus_tag="IVD64_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6147559.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="IVD64_000283"
     CDS             complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="IVD64_000283"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP6147560.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="IVD64_000284"
     CDS             complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="IVD64_000284"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP6147561.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="IVD64_000285"
     CDS             complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="IVD64_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP6147562.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="IVD64_000286"
     CDS             complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="IVD64_000286"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP6147563.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="IVD64_000287"
     CDS             complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="IVD64_000287"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP6147564.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39817..41376
                     /locus_tag="IVD64_000288"
     CDS             39817..41376
                     /locus_tag="IVD64_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP6147565.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41487..43418)
                     /locus_tag="IVD64_000289"
     CDS             complement(41487..43418)
                     /locus_tag="IVD64_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6147566.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="IVD64_000290"
     CDS             complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="IVD64_000290"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP6147567.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44347..45099)
                     /locus_tag="IVD64_000291"
     CDS             complement(44347..45099)
                     /locus_tag="IVD64_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6147568.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45258..45467)
                     /locus_tag="IVD64_000292"
     CDS             complement(45258..45467)
                     /locus_tag="IVD64_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147569.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45478..46806)
                     /locus_tag="IVD64_000293"
     CDS             complement(45478..46806)
                     /locus_tag="IVD64_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147570.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47171..47758)
                     /locus_tag="IVD64_000294"
     CDS             complement(47171..47758)
                     /locus_tag="IVD64_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP6147571.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47841..49091)
                     /gene="clpX"
                     /locus_tag="IVD64_000295"
     CDS             complement(47841..49091)
                     /gene="clpX"
                     /locus_tag="IVD64_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP6147572.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49338..50627)
                     /locus_tag="IVD64_000296"
     CDS             complement(49338..50627)
                     /locus_tag="IVD64_000296"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP6147573.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50781..51713)
                     /locus_tag="IVD64_000297"
     CDS             complement(50781..51713)
                     /locus_tag="IVD64_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147574.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51870..52529)
                     /locus_tag="IVD64_000298"
     CDS             complement(51870..52529)
                     /locus_tag="IVD64_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP6147575.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52532..53557)
                     /gene="sppA"
                     /locus_tag="IVD64_000299"
     CDS             complement(52532..53557)
                     /gene="sppA"
                     /locus_tag="IVD64_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP6147576.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53570..53860)
                     /locus_tag="IVD64_000300"
     CDS             complement(53570..53860)
                     /locus_tag="IVD64_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147577.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54269..56074
                     /gene="glmS"
                     /locus_tag="IVD64_000301"
     CDS             54269..56074
                     /gene="glmS"
                     /locus_tag="IVD64_000301"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP6147578.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56146..57501)
                     /locus_tag="IVD64_000302"
     CDS             complement(56146..57501)
                     /locus_tag="IVD64_000302"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP6147579.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57525..58694)
                     /locus_tag="IVD64_000303"
     CDS             complement(57525..58694)
                     /locus_tag="IVD64_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147580.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58691..59578)
                     /locus_tag="IVD64_000304"
     CDS             complement(58691..59578)
                     /locus_tag="IVD64_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP6147581.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59767..63459)
                     /gene="nifJ"
                     /locus_tag="IVD64_000305"
     CDS             complement(59767..63459)
                     /gene="nifJ"
                     /locus_tag="IVD64_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP6147582.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63658..64179
                     /locus_tag="IVD64_000306"
     CDS             63658..64179
                     /locus_tag="IVD64_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6147583.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17833 a        13921 c        11458 g        20975 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 tttaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
     7801 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
     7861 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
     7921 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
     7981 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
     8041 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
     8101 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
     8161 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
     8221 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
     8281 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
     8341 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
     8401 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
     8461 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
     8521 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
     8581 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
     8641 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
     8701 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
     8761 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
     8821 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
     8881 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
     8941 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
     9001 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
     9061 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta
     9121 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
     9181 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
     9241 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
     9301 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
     9361 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
     9421 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
     9481 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
     9541 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
     9601 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
     9661 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
     9721 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
     9781 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
     9841 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
     9901 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
     9961 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    10021 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    10081 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    10141 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    10201 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    10261 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    10321 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    10381 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    10441 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    10501 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    10561 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    10621 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    10681 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    10741 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    10801 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    10861 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    10921 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    10981 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    11041 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    11101 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    11161 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    11221 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    11281 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    11341 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    11401 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    11461 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    11521 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    11581 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    11641 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    11701 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    11761 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    11821 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    11881 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    11941 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    12001 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    12061 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    12121 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    12181 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    12241 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    12301 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    12361 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    12421 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    12481 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    12541 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    12601 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    12661 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    12721 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    12781 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    12841 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga
    12901 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    12961 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    13021 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    13081 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    13141 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    13201 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    13261 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    13321 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    13381 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    13441 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    13501 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    13561 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    13621 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    13681 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    13741 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    13801 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    13861 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    13921 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    13981 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    14041 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    14101 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    14161 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    14221 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    14281 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    14341 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    14401 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    14461 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    14521 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    14581 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    14641 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    14701 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    14761 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    14821 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    14881 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    14941 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    15001 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    15061 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    15121 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    15181 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    15241 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    15301 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    15361 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    15421 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    15481 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    15541 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    15601 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    15661 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    15721 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    15781 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    15841 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    15901 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    15961 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    16021 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    16081 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    16141 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    16201 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    16261 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    16321 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    16381 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    16441 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    16501 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    16561 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    16621 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    16681 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    16741 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    16801 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    16861 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    16921 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    16981 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    17041 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    17101 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    17161 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    17221 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    17281 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    17341 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    17401 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    17461 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    17521 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    17581 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    17641 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    17701 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    17761 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    17821 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    17881 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    17941 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    18001 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    18061 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    18121 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    18181 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    18241 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    18301 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    18361 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    18421 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    18481 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    18541 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    18601 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    18661 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    18721 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    18781 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    18841 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    18901 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    18961 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    19021 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    19081 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    19141 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    19201 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    19261 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    19321 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    19381 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    19441 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    19501 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    19561 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    19621 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    19681 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    19741 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    19801 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    19861 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    19921 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    19981 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    20041 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    20101 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    20161 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    20221 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    20281 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    20341 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    20401 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    20461 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    20521 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    20581 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    20641 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    20701 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    20761 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    20821 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    20881 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    20941 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    21001 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    21061 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    21121 tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt
    21181 tttatgaaac tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt
    21241 ggttttattg aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt
    21301 tcatgacata tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct
    21361 aaagattgct actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa
    21421 ggtgactcaa catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa
    21481 atcgtaagaa ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt
    21541 tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc
    21601 aacgaaaaat cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct
    21661 acccatttcg tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg
    21721 agtcctgcca ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc
    21781 cctgataggc cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc
    21841 aatgcgggta attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta
    21901 ccctctccag cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc
    21961 agtgtaaaaa tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact
    22021 ggagcaatcg gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt
    22081 ggatacaatc cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca
    22141 ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg
    22201 tttttattga gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc
    22261 gatttgacac ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga
    22321 cttttattta ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg
    22381 tccatctgca aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa
    22441 gtcaccgcca tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg
    22501 atctttaaaa tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat
    22561 gtaagaatcc attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa
    22621 cggtttttcc gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca
    22681 gctttttaca tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg
    22741 gacaagtcga tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc
    22801 cgcataagat tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc
    22861 gaaattcgtt tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac
    22921 gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt
    22981 ttccgccatc acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg
    23041 gtaatgagat gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc
    23101 catatcttct atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc
    23161 gttgataatc atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg
    23221 aaaaccgaag gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa
    23281 ttcaagctta tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc
    23341 gatgcgaatc gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc
    23401 aggatctagt atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc
    23461 tacttcaagt ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc
    23521 taaatggttt tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa
    23581 aaagtcctga tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat
    23641 attttcttct tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc
    23701 atacccgata aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg
    23761 aatcgagatt tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc
    23821 aaccggattg tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt
    23881 gaaaggggga cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat
    23941 cttgtcataa tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc
    24001 atcaaaatct tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa
    24061 acgccatgat ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt
    24121 tgcataacaa acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc
    24181 atctaaccat tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa
    24241 tgaagcagtt ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc
    24301 aattcggccc ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct
    24361 aaccccatca gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa
    24421 aacacaatag cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca
    24481 aaaggactcg ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag
    24541 gatataattt ggtcttttac ttgcttttct tcttcaggag acagctttgt caaatccagt
    24601 tcataaccaa aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg
    24661 tcggtctgat gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca
    24721 aggcttgttc catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat
    24781 gactgcggca tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct
    24841 tcccataaga tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc
    24901 atataacgat gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct
    24961 gaaagcgaac ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt
    25021 ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt
    25081 gatctggaag cagaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga
    25141 tacagttcag aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct
    25201 ttgctatgag cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg
    25261 aaccagtctc ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat
    25321 acgaacattt cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta
    25381 ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga
    25441 tgacgacctc ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc
    25501 aatccctgac ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc
    25561 aattcccacg aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga
    25621 tctttttcaa gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct
    25681 ccagtatttt ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg
    25741 ctcgttcctc gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc
    25801 tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg
    25861 gcaaagtcta gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt
    25921 cttatcttta cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg
    25981 aaataaatcg ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg
    26041 tccatcacat aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg
    26101 ctgtcatatc tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc
    26161 gttcgaaagt ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga
    26221 gaccggtcag gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt
    26281 ttattgtctt tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt
    26341 tcttctatct ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt
    26401 tcatcaaata agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc
    26461 ccaacaaaac ggaaaccgtt tacttttttt tataattcct gtatactgtt tactaaacaa
    26521 ttttgtcaat aaaaattttt tatttttttg aggagtgtgt aaaaatggtt ggaattagag
    26581 atgtcgcacg aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg aataacgatc
    26641 agacgatcca tgtggctcag actactcggc aacggatagt agaggcagct gctgcgttgg
    26701 attatgacat cagtactcgt aaatatatca aaaagagaat gccttcgatt ggtgtgatct
    26761 ctacgatcgg caaagcgagc gaagaacagg atatctatta caaacatttg cgagtaggga
    26821 tggaagaaga agctcgacgt ctgcatttag ggatgaatcg aatctataat ctatctgata
    26881 atcctaaaca atgggaagat ctagatcagt taggtgcggt gatcgttgtt gggactgtta
    26941 cgaaagaatc gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc gtggataacc
    27001 ctgacatcca gcaagaggtc gatatggtgt atgcagatct tgaacggatg actaaaaccg
    27061 tactggaatt gttcttagaa aatggtcata aaagaatagc ctatatcggg ggatataaag
    27121 tcgatatgga tgaattcggt gggaagcaga tttctataaa tgaaaagagg atgcgtgcct
    27181 atcagcattt tatgatcgat catcacttag aaaaatatat gtcttatcat ctaggtggct
    27241 ggacagaaaa acaaggtgag gtacttgcga ctaatctatt agaaaatgaa tcccctcttc
    27301 ctactgccat tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg gcattgaaac
    27361 aaaaaaatat ccaaattgga gaagagattg ttgtagcaag ctttgataat atcgagacaa
    27421 tcgcaaacct ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag
    27481 cagctgttcg aatggcattg gagcgaattg acggtatacg ggacgaacca atcattttga
    27541 cttatcctgc acgattgatt gttagggaaa gttttatccc aagaacaaaa aggtattgaa
    27601 agagaggttg tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg ctttttcaca
    27661 acctcttact tatacttatt cacaaaatcc aatgtctgct ggtaacaatt ttgcgattct
    27721 tttagcgtgt aatcaaattg gtattcatgg gtaatctctt tttttgtatc tgtataaaac
    27781 aaggattgca cgggtacctt taattctgtc aaccgatttt ctaatgcttg accttgctcc
    27841 tggaatgagt atgcatttcc gtcagttata tacgttggcg gaaattcctt agaaacatgg
    27901 tcagccacac tagcttcttt caattgctca ctcgttttcc aatcttttgt tccaatcaac
    27961 gaccacgcaa cggttttgac gaaaaacttc ataaatcgat catccgaatg ctgctgtgct
    28021 gttagcttca aatcaacagg tccgcaataa gaaatggttc ctttcagact atcttttgga
    28081 agtagatgtt tgattttcat ttgtttagca tacgcatcat tcgtttgtat agctgcatat
    28141 tgcaatgcga tttgtgaacc tgcactatct ccaccaaaaa ataattgttc catgtctagc
    28201 aatttgtcgt ctttttcttc agctaataaa tctttgatca gttcattcac ttgaagaact
    28261 tgattagggt attctgaatc aggcgctaat tcataattca tcgcaaccac tgcgatgttg
    28321 gcgtctgaaa ctaattttgt cgcaaattct ttgacacccg atttatctcc gccgacgaat
    28381 cctccaccat gtacccaaaa tagaacagga acaggcccct tgctgttttt tggatagtag
    28441 atgtcataag tattctcttt atacttggac tcatacgtca aatctgtttt tttcgccacg
    28501 tttttttctg cttgatcgaa cgttttttta tcagtgattt gaacagtatt tgaaaacata
    28561 tggttgatca agaatgctcc aggtctgggg gaaagctgga aggcaagaaa gattgcaaac
    28621 gcgatcccaa gaatgattcc gaagaaccat ccgataaatt taagaatttt ctttttcata
    28681 aggctccctc agtagttagt attaagcatt ctgattatac catagtgtta tttcctatca
    28741 agctctccta gatgatttaa tgattttccc caaacttctt ctgcaatgct tttgacaaaa
    28801 gaaatttttt ccattgttct tcaaacaaaa cattttcttc ttctgttgaa gcaaatccac
    28861 attgaggact taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg tagatccggt
    28921 caatcacttg ctggtgctct tccatttcgc catttttcgt tacagtcaaa atctaagaca
    28981 actaatttat cttcagagac tttctctaaa agaacaaaac tcccagcacg ttcactatcg
    29041 tattctaaat aataggcacg gacattttct tgagtaaaat aatgggtctg ctacgtcgtc
    29101 atcccccctg atgccgccaa aatcgaataa taatttccgc gtcaaacatg tatatttagt
    29161 gttgaatcat ttgagaatat gaaaataaaa atctttttga tcaatcagtt aatcaaattg
    29221 aaataacgaa aaatctgaga cagaatgacg ttcattctga cccagatttg attttgcaca
    29281 actcaacagt ccttattggc aaatcgccaa aataatgtag actaactaaa aagactaaga
    29341 aacaataaca aaagttaaca cttcttagtc tttctttagc acgtctattt attaaagatt
    29401 ttagggcctt ttctcatgtt tttacgatca ttcttaggtg tttccatttg tttggatcgg
    29461 cctccacctt gtttcttttt gatcaattct ttcatttttt ctttttggtc catttttatt
    29521 ctcctttaac gaatgaagcg actaattcga tatgatgggt ttgcgggaaa agatctacag
    29581 gctgaacttt cttcacggca taacctgctt ctgcaaataa cttagcatct cttgcaaaag
    29641 ttgccggatt acatgagata tagacgattc gttctggtga catttcgatt gctgattcaa
    29701 taaaatgatg atctaatcct tttcgtggag ggtcaactac gatcacagat ggtttgatcc
    29761 ctttttcttg ccattttttc ataacttttt ctgcttttcc gacttcataa tgaacatttt
    29821 cgattttatt atttaaagca ttgaactgtg catcttcaat tgcttctgga ataacttcca
    29881 tcccatacac cttatccact ctgtctgcta aagacaagcc aatcgtacct attcctgaat
    29941 aagcgtcgat cacaatgtct tttttatcta actcggcaaa ctcgatggct gtttggtaca
    30001 gatactctgc ttgttgagta tttacttgat aaaatgactt tgctgagatt cgataggttt
    30061 ttcctaatag ctgatcttcg atatagtctc tgccatataa aactttttct ttgtctccta
    30121 aaatcacatt tgttttttct tcattgatat tttggatgac cgaaaccact tctggcaatt
    30181 cttcatgaat agcttcagca atttccttgg atttaaagaa tttttcttta cgagtaacta
    30241 aaacgaccat catttcatgg gaataatgac cacgacggat aatgatgtga cgaagctgtc
    30301 cttcattttt ttcttcatta tatcctcgta cttggaaccg ttgcaagata tctctgacta
    30361 ctttgattgc ttgatcgatg acaggatctt ggatataaaa atcttcgata gggactagat
    30421 catggctatt ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg cgaacaggga
    30481 tctgtgcttt attacggtag ccgaatggtt ctgccattcc gaccgttgga agtacaggaa
    30541 cttctggcat cttagcaatc ttcgccatta catgctctac ttgtttttgt ttgaagacaa
    30601 gctgttgttc atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt aatgcattgt
    30661 tttctacagg ctgacgatca ggtgattgtg taataaatgt ttcgactttt ccatatccaa
    30721 acttattgcc tgcttttact actagtattt ctacttcttc tccagggagt gcattttcga
    30781 taaataatgg ataaccatcg atttttgcta cccccattcc ttcataactc aagtcgacga
    30841 tttcgactgt atatcgttcg tttttcttta ctatttgctg ttctgtcata ttttgctcct
    30901 ttaagcctat ttttcaatcc tactgaactc tactataaat cagctaatta cacaactaaa
    30961 accggcactg accttactac gttcagcacc ggcttttgat taattctttt gattaggtgt
    31021 ttcgtctgct atttttccgt caccgtcgat atcttcatcc gttaaacgtt cgacttcttt
    31081 tacgaattct ttgctgacaa cctctaattc ttcttcatct gtacctgtga ttgcttcatc
    31141 aggaatctga tcggtattgg caaagaactc aatgtgttga tgaagatttt ggaaatgcat
    31201 cggtgcttcc ccgccgtatt caccatctaa gttgatcatt actggtcgat tgatatcatc
    31261 ttctgtttca acgaacaaac tgcttgtttt ggtatagatt attcttggat catcgacatg
    31321 tcgaccgcca ttcagcatga gtgctgcaat atgcaagatc tcaaagatat ttgctgtctt
    31381 cacaatgatt aaagaaaact tcccatcatc caatttggca tctggcgcga tctgttcgaa
    31441 tcctccaaca gagttcgtca gccctaggaa aaacatcgaa gcatttccga tatattcccc
    31501 ttcatcatac ttcaagcgca ttttgattgg ctttactcga gggagcatct ctgctccttt
    31561 cgcaagataa gcaagataac caaaaatact ctttagttct gatggaactt cataagtcaa
    31621 ttctgtcaaa tagccacctg cagcgatatt gatgaagtaa ctgttatcag aaaggccgat
    31681 gtccattttt acggtttggt gtttttttat cacttctgct gctgctttaa tattatctct
    31741 aggaattttt aatgctcgag catagtcgtt cgttgtacca gctgggatga tggccatttt
    31801 aggacggtgt tcaagcggag cgattccatt aaccacttcg ttgattgtgc catcgccacc
    31861 agctgccact actagatcaa aacctagttc tgcgactcta catgcttcat tcttggctga
    31921 atctggttct ggtgtagttg cataggcgct ggcttcaaat cctgcttctt ctaatgcctg
    31981 taggatatcc gccaaatttt ttttcaataa ttcttttccc gaggtggggt tataaattac
    32041 gcgtgctttt ttcattttca gcctctatcg tttcgctaat tcatctttaa gtagcttatt
    32101 cacgacacca ggatttgctt gtcctttagt tgctttcatg atttgaccga ctaggaagcc
    32161 aaccgcacga tcttttccat ttttgaaatc atcgacagat tgttggttgt tatccaaaat
    32221 ttcattaatg attggcagta gtttgttcgg atcagataat tgcacccaac catttttttc
    32281 aactacttct tgggcttctc cgccatttgt gatcaattca cggaagactt ttttggcgat
    32341 ttttgagctg atcgttccat cggcgatcaa acgaatcatt cctgctaagt tctcaggtgt
    32401 caattttgtc tcagctaatt ccagtttctc actgttcaag taagcagaaa cttcacccat
    32461 caaccagtta gatgcttgct tggcatctgc accattttcc aaaacagctt caaaaaaatc
    32521 agacatttct tttgtaagtg tcaataccat tgcgtcatac tctggaagtc caaactcacg
    32581 gatatagcgt tcgcgacgag aagctggcat ctcaggcaaa ctagctttta ctttttgaat
    32641 ccattcgtca tcgatcacga atttcgggat atcaggctct gggaagtaac gataatcact
    32701 agacccttct ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa aacggcgtgt
    32761 ttcttgttgg atttctcctc ctgaaagaag aacttttgct tgacgttttt cttcaaaagc
    32821 taaacctttt ttcacgaaag ataaagagtt caagtttttc aattctgctt tcgtaccgaa
    32881 ctcttcttgt ccataagggc gaagagaaat attggcatca cagcgcatag agccttcttc
    32941 cattttcaca tcgctgactt ctgtaaattg gataattgat cgcaatgctt ctagataagc
    33001 gtatgcttct tctggtgaac gcatatcagc ttcagaaacg atttcgatca aaggcgtgcc
    33061 ttgtcggttc aaatcaacgt aagaataacc gccaatacca tgcatgtttt ttccagcatc
    33121 ttcttctaaa tggacacgtt cgatccggat ttttttcttt ttaccttcta cttcgatttc
    33181 gatccatccg tcatgaccga ttggttgatc aaattgggaa atttgatatg cttttggatt
    33241 atctgggtaa aagtaatttt tccggtcaaa atgagtatct ttagagattt cacagtttaa
    33301 tgccaaagct gctttcatcc cgaattccag cgcagcttta ttcattactg gcagcacgcc
    33361 tggataaccc cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa aatgagctgg
    33421 cgctggagag aaaatttttg agttggtttt taactcgacg tggacttcta gtccaatgac
    33481 tgtttcaaaa ttcattcgtc tttccctcct aaaatagctg gttgttgttt atggaattct
    33541 gttgcttgtt caaacgcata tgccgcttga tacatcgttt tttcatcaaa ataattaccg
    33601 atgatctgca atccaactgg taatccttct gataaacctg ccggtacaga catacctgga
    33661 agtccagcca agttgacagg gatcgttaag atgtcactca tgtacattgt gattggatcg
    33721 ttaatgttct caccgattcc gaatgcaact gttggtgaag atggcccgat gatcaagtca
    33781 taatctgcaa agactttttc aaaatctcgt ttgatcaatg tacggacttg tcctgctttt
    33841 ttgaagtaag cgtcataata acccgcgctt aaggagaatg tacctaacat gatacggcgt
    33901 tttacttctt caccgaatcc ttctgaacgt gagttgacat atacatcatc taatgatttc
    33961 acattttcag agcgataacc gtagcggata ccatcgaatc gttgcaaatt ggaacttgct
    34021 tctgatgagg caatgatata ataaacagcc acaccgtatt ttgaatgagg caagctgact
    34081 tcttccacaa cagcacctaa tgaacggaat gtttctgctg ctttcaatac agcttctttt
    34141 acgccagggg ccacaccttc gcctaagtat tctttaggta aagcaatctt cattcctttg
    34201 atgtcgcctg tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga agtaccgtct
    34261 ttttcgtcat aaccgctgat agctgtcaat gctaatgcat tatctttcac cgtacgggtc
    34321 attggtccga tttggtcaag agatgaagca aaggcaatca aaccaaaacg tgagacacgg
    34381 ccataagtag gtttcatccc tacgatgccg ttaaaagctg ctggttggcg gatacttccc
    34441 ccagtatcac tacctaaaga aactggaatt tgtccagctg caactgctgc tgcagaacca
    34501 ccagaagagc caccaggtac ttttgttggg tcccaagcat tttttgtttt cttgaaataa
    34561 gaagtttctg tacttccacc catagcaaat tcatccatgt ttagcttacc aacagggatc
    34621 atatctgctt gatagatttt atccatgacc gttgcatcat aaattggttc aaaattgtaa
    34681 aggatcttag atgcagcggt tgtcaagata tcttttgtta cgatattatc cttgatacca
    34741 attggaatac cagcaaggac gttgctttca tcaatccctt tttcatctaa agcttttgcc
    34801 tgttccaatg ctttttcttc gttcaatgta atgaatgcct cgacctcagg ctctgtttct
    34861 ttgattcgtt caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt cgatacaagc
    34921 atatgatgca gttcttctag agaacgattg tgtaattctg tcattatgcg ccagcctccc
    34981 cattatcgat gattgcgggt actttgataa agccgtcttc tgtttccggc acatttttca
    35041 ataattcatc gcggttccat ccagcttgcg cccgatcttg tcgcatcacg tttactgttt
    35101 caaccacatt cgacgtaaat ggaacacctt ccgtatctac ttcttccagc atttcaacca
    35161 tatcgatgat tttacctaat tggtcggtaa aacttgcaag ttcttcatct gcgaacgcaa
    35221 gttttgctaa tttggcaacg tgtttcactt gttcttcact aatcgccata ctaccccttc
    35281 tttctaaacg agttaaaaac tttgctctct attttaacac aagagtgccg tgtctgaaat
    35341 tgtttattga aatttgtctg aaaaaactca tttttcattc ttctactgag cgacttttag
    35401 ccaatgcatc tgccatttga tcttctgtcc aaacttcgat tcctaattct tgtgctttgg
    35461 tcagcttgct gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac
    35521 cagtcacttt cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg
    35581 ttagtttacc agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt
    35641 gggcatttcg aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca
    35701 attcatggac ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga
    35761 tcgtatcaag cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga
    35821 aatgttctgc taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa
    35881 tcaaccgctc gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg
    35941 atttttcttt tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag
    36001 caacatctgc tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat
    36061 cgatattcat cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc
    36121 atttaggatt gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac
    36181 aaactggaca atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca
    36241 cgacttgcga aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca
    36301 agcggatatc cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg
    36361 ttcctgctac tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga
    36421 ctgtccattc gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg
    36481 ctgcccatct cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca
    36541 ctttgatgac gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt
    36601 caatgtatga ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga
    36661 atccaatttt ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga
    36721 aatcagctac tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta
    36781 actgacgcaa agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg
    36841 cttctctgtc ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta
    36901 cttcgatgtt cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt
    36961 cagtgatgtt ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat
    37021 cttcataacg caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta
    37081 cacgtcccac tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa
    37141 cgtcgttcaa actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt
    37201 ttccgcctac tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga
    37261 ttgctactaa ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt
    37321 ctacgtagta ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag
    37381 cagcttcgtc aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca
    37441 attggcgcaa aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt
    37501 tctagatcct tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt
    37561 ttgactttat ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga
    37621 acaaccgctg tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt
    37681 tttttaggcg taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca
    37741 aaacgagaag gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt
    37801 aaatacaacg cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc
    37861 agttcgcttt cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc
    37921 aggaatacaa ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg
    37981 gaagcatctt cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc
    38041 gttagttttt cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg
    38101 aattcttgtg tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat
    38161 gtattttgga tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat
    38221 tctgtcactg tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca
    38281 cctaattgct gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca
    38341 agcagtggcc attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg
    38401 atgcctcgtt tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc
    38461 agcacattca aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct
    38521 ccaaccattt tataaggaat atttgctttc aacagagttt cttccattac acgagattgt
    38581 gcgttcgtcc ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct
    38641 tcttgcatcc ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa
    38701 gtgatcttgt ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg
    38761 ttttcaatga cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct
    38821 agcaaaatta ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct
    38881 gcaccgcgcc agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat
    38941 ctacctgcca ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct
    39001 acatgaatat aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat
    39061 aatcgaatcg tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct
    39121 ttttggtaag cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt
    39181 gcaaaatcct gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt
    39241 cgaggatcat attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc
    39301 agctgttcag aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct
    39361 cttcttaaaa tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca
    39421 gatgcgagaa tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta
    39481 atcgctagga tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt
    39541 gtcagcactc tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg
    39601 tgaagaacag cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac
    39661 attaacaaca tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt
    39721 aaactagcta gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg
    39781 atttcgcgct aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag
    39841 aaattagata agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca
    39901 ggaatcgtcg ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt
    39961 atctatgaaa tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc
    40021 cttgcagcat ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg
    40081 attccacaag tagtgggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta
    40141 tggaaaaagt tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa
    40201 ggaccttcta tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt
    40261 ccatcttcag aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca
    40321 ttcaatgctc ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct
    40381 ccacttgttt ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac
    40441 tttttcggct tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat
    40501 tatggttcct tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat
    40561 gtcttgctgg ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg
    40621 ttcgttcctt tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga
    40681 ggcaatcaaa tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt
    40741 ttgtttgttc ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg
    40801 attttccttc cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg
    40861 gttgatttcg ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt
    40921 tatttcacag cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt
    40981 ggctctctta atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat
    41041 gaccttctcg gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact
    41101 cagaccgcct cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt
    41161 tctttagacg gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct
    41221 atacgcagag gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat
    41281 attttggtta tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct
    41341 aaatcttcta tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca
    41401 acaactattc atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg
    41461 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
    41521 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
    41581 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
    41641 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
    41701 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
    41761 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
    41821 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
    41881 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
    41941 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
    42001 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
    42061 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
    42121 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
    42181 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
    42241 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
    42301 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
    42361 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
    42421 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
    42481 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
    42541 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
    42601 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
    42661 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
    42721 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
    42781 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
    42841 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
    42901 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
    42961 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
    43021 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
    43081 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
    43141 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
    43201 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
    43261 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
    43321 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
    43381 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
    43441 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
    43501 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
    43561 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
    43621 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
    43681 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
    43741 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
    43801 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
    43861 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
    43921 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
    43981 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
    44041 agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt
    44101 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
    44161 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
    44221 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
    44281 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
    44341 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
    44401 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
    44461 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
    44521 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
    44581 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
    44641 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
    44701 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
    44761 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
    44821 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
    44881 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
    44941 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
    45001 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
    45061 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
    45121 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
    45181 tataaatcag tcaaaaaaaa ataaaaaacc agtcagaaag actggttttt tatttttttg
    45241 tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt catataaaca
    45301 gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc gccaaacatg
    45361 accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag acagacggat
    45421 aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt cttcacctca
    45481 tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt gcacacgcag
    45541 atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct ttattcgttt
    45601 gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg acagggattc
    45661 tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat ctgttacctc
    45721 tacttctaga tattccctaa tataataaca ataagcttcg attccattat ccaaagcaac
    45781 agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa cccaaggaaa
    45841 atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc cttgttggat
    45901 atcataactt tttgttacaa aaaatgaagc attcttttta ggaacaggtt gcatttgtgc
    45961 attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa cgaatggcgg
    46021 tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct ggtccctaaa
    46081 ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc gataaagctg
    46141 gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga tgtcgagaaa
    46201 aatcggttta gggatgattt gatgataata gcgactataa tacatttccc aacacgaagt
    46261 gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat cgactactac
    46321 ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt tctcaaacaa
    46381 tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag ctcgcatata
    46441 ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca aaaattgctt
    46501 ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat cttccaagcg
    46561 aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc ttaccgatgc
    46621 tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt gtgtgaaaga
    46681 gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc ttatttcctg
    46741 ttttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt tatcttcgcc
    46801 aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa tgataaagaa
    46861 aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga ctaagtaaaa
    46921 agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa tttagataaa
    46981 cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga agagatcgat
    47041 cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt
    47101 aagctcaaaa aaagacttac cagccaaact tttatgaaag agtttgactg gtaagtcttt
    47161 tttattcttt ttatgattca attgcttgtt cgattgctgc ccacgcttct tcttttccct
    47221 gttttgtaac ggaagaaaag agaataaacg tgtcattgcg atcaaaatcc aacgcttttt
    47281 tgatttcgct ctcatgcttg ttccatttac cacgagggat cttatctgcc ttagtggcaa
    47341 caacaattac agggatatca taatacttca aaaattgata catctggata tcttcttgag
    47401 aaggcttatg acgcaaatct actaaagaaa cgaccgcacg tagctggtct ctagtcgtta
    47461 aatatgtctc gatcatcttt ccccattttg ctcgctcagt tttggatact tttgcatacc
    47521 catatcccgg aacatcgaca aaatgtaatg cgttctcgat caaataaaaa ttcaatgtct
    47581 gggtctttcc tggcttaccg gaagtacggg ctaaattctt tcgatcaatc aatgtgttga
    47641 taaaagaaga tttccctaca tttgatcgtc ctgccagagc tatttctggc aattgtgtat
    47701 ctggatattg ttttggtgcc actgcactta tgataatttc agcttgatgg actttcatgg
    47761 tctctctcct taacaaggtg atgaataaag caagcggctg tcgcaaaagc caaaatgact
    47821 tttacggcag cctcttttca tcaacccgct tttttctctg aatctttata gattactgtt
    47881 ggctcaccag acaattcagc tgcttctttt gtgataatca ctttttcgat gttttcattt
    47941 gaagggatat caaacatcac atccatcatg atttcttcaa tgatggaacg aagtcctcga
    48001 gcaccggtat tgcgttcgat tgcttttttc gcaatggctc tcaacgcttc aggttcgaat
    48061 tcaagttcgg tattatctag agaaagaagt ttttgatatt gtttgactag cgcatttttc
    48121 ggctcagtca agatccgtac cagatcatct gttgtcaatt tctctaaagc tgccataaca
    48181 ggtaatcgtc cgataaactc agggatcaat ccgaatttta gtaaatcttc aggaatgata
    48241 tgctgcatga cactttcgtc ttcagaaagt ttttggtttt gtgtaccaaa tccaatcgtc
    48301 ttttcgccca tacgattctt cacaatcgtt tcgatgccat caaatgcacc acctacgatg
    48361 aacaatatgt tcgtcgtatc aatctgaata aattcttgat gtggatgttt acgtccgccc
    48421 tgaggtggca cacttgcaac agttccttct agaattttca gcaaggcttg ttggacacct
    48481 tcgccggaaa cgtcacgagt aatagataca ttttcgcttt tacgcgcaat tttatcgatc
    48541 tcatcaatat agatgatccc tttttctgca agttccacat tgtaatcagc agattgaagc
    48601 aatttaagca aaatgttttc tacatcttcc cccacataac cagcttctgt caaacttgtc
    48661 gcatctgcga ttgcaaaagg tacattcaat gtttttgcta atgtttgtgc taaaaatgtt
    48721 ttaccagacc ctgtaggtcc gatcaagcag atattgcttt tttgcagttc aacatcatct
    48781 gtgttttctt gtgcttgctg attgacacgt ttatagtgat tgtatacggc aacagctaac
    48841 gagcgttttg cgcgatcttg tccaatcacg tagttattca atacttcaag aatttctagt
    48901 ggtttaggga cttcagtgaa ttcgcgtaca gcttcttcat aaaattcttc atcaatgatt
    48961 tctttacata ggtcgataca ttcattacag atgtagaccc ctggaccagc tacgatcttt
    49021 ttcacttctt cttgggtttt cccgcaaaaa gaacagcgta cggtctcatt gctacttgga
    49081 ttgtcataca tggtcttcac cccttaactt gcaaaaagta tgcaagaaac tatcaaagac
    49141 tttacagtct tttactatca taacatagtt tagtaaaaag aaaaagtgtt taacaaagga
    49201 tcaactgacc tatacctttg cattttttac tcttaacaaa aaataaggaa ctgtgacaaa
    49261 atcgtgtcac agtcccttat ttcgaataaa caattttcta gaagtagctt cttcagaaat
    49321 gatttattac ttgatttcta ttctttcgct tctgtcgctg tacctgtgat caattcgatt
    49381 gcttttttca ttgtcacatc atgttccagc atatcttctg tcaacacgcg tttgatttgt
    49441 tcgattggca tgttgtatgt ttctgataat tcttggattt ctgcattgat ttcttcttct
    49501 gttgcttgaa gattttcagc agctgcaatt gcttcgatca caagatttgt tcttgtgcgc
    49561 atttctgctt caccttcgaa ttgtttatgc aagtcttctt ctgtagaacc agttaattga
    49621 tagtacattt caggagagat accttgacgt tgcatgttgt tcaagaattc gtccattgaa
    49681 cgatgtactt catcatgaac catcacgtgt ggcaattcaa cgatttctgc gttttcaaca
    49741 gccatacgga tcgctgcttc atcttttgca tcgtccgctg ctttttcttt tgtttctact
    49801 aattctttgc gatatttttc tttcaattcg tctaatgttg atacttcgtc atcaacatct
    49861 tttgcgaatt cgtcatctaa ttcaggcact tcttttgctt ttacttcatg gattgtcact
    49921 ttgaagactg cttcttttcc agcaagatct tctgcttgat aatcttctgg gaaagttaca
    49981 tttacatcta gactttcacc tgatttatga ccaactaatt gatcttcaaa acctggaatg
    50041 aatgagcctg aacctaattc aagtgagtaa ttttcgcctt ttccgccatc aaacgcttcg
    50101 ccatctacaa agccttcgaa gtcgattact actgtatcgc cattttcagc tgcagcatct
    50161 tctttgataa ctaattcagc ttgtgcttct tgttcacgtt tgatacgtgc atccacgtca
    50221 gcatctgtta cttcacggtc ttgtttttca acaactagat ctttatattg tcctaatttt
    50281 acttctggtt tcactgttac ttcagcagtg attacccagt cttgaccttt ttccatgctt
    50341 tcaacatcga tttttggttg agaaacagga tcgatgccag cttctttgac agcagcttca
    50401 taagcttctg gtaatacagc attcaatgca tcttcatata aagcttcttc accgtacata
    50461 cggttgaata cttgacgaga aactttccct ttacggaatc caggtacgtt caagcttttt
    50521 ttcactttat taaacgcagt tgttaatcct ttttggattt cgtcttgcgc aatcgtaaat
    50581 tttaaaacac catcattcgt gccttttttt tcaaatgttg cagtcattta attccctccg
    50641 agtttagaaa ttcattttat acatcaaaaa cgtttcatgc atacctttaa atagtacact
    50701 aaccctttca aattgtaaac atattgaatg gatagaatcc ttgtttttca taacattttt
    50761 atctttttca ctgataaaat ctaatggaag aagatcattt gctgcaaaat tttttctctt
    50821 ttttttaaaa aatcttgttt ttcatcttct gtaccttcat acatcgtttt tcctgtacac
    50881 gcctctacag tcagttgaaa ccagcgcttc gggtcttcga tgtatgtatc atagacagga
    50941 taaacaattg cagattgcaa cgtgaattct tcttttaaca cggaactaag cagcgcatct
    51001 tcattcccgt aacgatctga aaatacacat aattgctggt aacttgcctg ctgctgcggc
    51061 tttggcagat cttttggtat gactttgact aacttttctt caatcgtcaa atagaaaaca
    51121 ggatggcatt cgttcaattg cctaagtgtc tccagtaatt ttgcccttgc taaaatatgg
    51181 acctctggcg atacaataag tttactcgct gtttggataa actcatgata aggaagctgt
    51241 tcgatctcat caatagacgc aagctgacgt gcaggttcca acgaaggaag ttcctccgat
    51301 ttattgtgga gtgccttgat cttctgcctc tccatttgtc cggataattc ttctgcaaat
    51361 tggatttttt caatgacccg ctgctcgaaa gcttcataaa aatctgtact tgctatcaat
    51421 tttctagcat ataaaaaatc ttgtgtactg attaacaagt caaaataaaa ttcagcaaac
    51481 tcctcatttt gaagatactg tttttcatgc aagagtgctt gcttcaatgc ttcttttttc
    51541 tcgtccaact cgtaaagaca aaaaacaagc aggctattag cctcaaagct ttctttcaac
    51601 tcgtaagctc gttgaaataa ttcttttgcc tgctgatatt gctggtttgc tagtgcttgt
    51661 tgtgccactc gcattaaatt ctcatatttt tctggaaaat caatcggctt cacgcagtgt
    51721 cctcctcttg ctcatattca gctcatgttc aatagtagca aaacaaaaaa agcatgacaa
    51781 ttaaaaataa ctgaagcacc ctctaaaagc tagattttag atctgacttt tagaaggtgc
    51841 tttcaactgt tactgctttt tattgctcat cagctgttga cttcctggtt gaacgctttg
    51901 atcagattga tcgtaacaac acttgtatta gtgaaaaaat cgcccagatg ttgctttttc
    51961 tgattaaaga ctgctggaag ataccctaac atcagcaaag gaaattttag aatgaaccgg
    52021 caagcacctt ctctaactaa aacggttgac cagctaagtt cttcttcatc aaagctgatg
    52081 actcgaatac caaagatcat tttcccaatc gtttggccat gattgaattt cgtcaataaa
    52141 ataaaatacg ctagataaat aagtaaggct gtgaatccgt agagactcag ataagaatct
    52201 gaaaaattaa gccaacctag atttttggca ataccaagtg ttaaacgcgt aatagctccg
    52261 atacacaaaa gatcgaccaa gaaggcaaac attcggataa aaaaacctgc atagaaatac
    52321 ttaggaaaat catttgcttt tggcggttca tttcttgtgt ccgtatactt tttccattta
    52381 cgttgtttct ttttgatttc ttcgggcgtc aaaggctctc tttcaaaaga gctcagtacc
    52441 ttttgggaaa agttggagtc aggtcctaca gcaggctgct gttgcttatc tttttcttgt
    52501 tccttatttt catttgaaag ctctgacatg cttactcacc tccgtaataa tacattgctt
    52561 ttggtgattc cgctgtccct agattctcaa gaacggataa gatctgacta gtctcagaag
    52621 gcttcaatcc ctggaattct gctagtttac ttcctaacca tgtactagca aacccagatg
    52681 gcgtggaaga atattctact agttccgcat cttcaagctg ttgttccttt cgtatagctt
    52741 ttaaagcatc ttcaggaaat cccaattcat cgactaaacc tacttctttt gcttggacac
    52801 catcataaat ccttccatca gcaatttttt tgacttcttc ttcagattta ttccgtccct
    52861 cgctgacgat tgctacaaaa cgactatatg cgctgtcgat atatgcttga agcaccgctt
    52921 cttcttcttt tgtctctggt cgcgttcctg aacccatatc tttcaacgct ccgcttttaa
    52981 ctgttgtatc ttccacaccc agtttttcta gaagtcctga ataattcaat cctgacatga
    53041 tcacaccaat tgagccggtc actgtttctt ctgttgcaaa gattttatct gcttgagcag
    53101 aaatatagta gccaccgcta gctgccatgt ttttcatgct gacatacatt gggatatcat
    53161 gttctttacg aatcgtatcc aatttcttag cgatttccgc actttcgtag acaccgccac
    53221 caggagaatt tacttctaaa aagatacctt tgactgttgg atcttcttca atcgccttca
    53281 gttgttccat aaaattttta tgattgtaac cttcagaagc aaataaacct gaagaccctc
    53341 ccgcagcaat cgttccatct actgataatt ttacgatttt ttgcgtactg tctccctctt
    53401 ccaacacatt tggagctagt tcttcatccc catataacca actgttcaat ccctcaattt
    53461 gatcgttttc ctgaggctta gtcaaactag ctgaaactaa cgaaaccaca aataatcctg
    53521 cagcaatcag aactgctgcc caacgtcttt tattcattct ttctcctttc taatacaagt
    53581 tttttatgac gatcacagtt tctgactgct cagaagtgtc gaaccgctct gctttttctt
    53641 cccgcgcata cccggaaaac gactcttttg ttaattcatc ttctaccgta aactcacgag
    53701 cttgagcaac ttccaaggca tcaggtgcga tttcttggat gctatcccag ccagtatcta
    53761 aaaatatttt cgtatcatct gatacaccag ctgatgcttc gaatttggac aattgttttt
    53821 ttaacattcc cagtgtcatg atctcaattt ttgaatccat ttttactcct cctctatccg
    53881 attactttct attgtaccga actcctgaaa aagatgatag ataaaaaccc attatttttt
    53941 gacataaaaa ggacatttga caaagaattt gatatatacc aatttatttt ttattctttt
    54001 tttgttgaca agaacattcg caaaaggtta tgattttaaa tgtttccaga taaaaaacaa
    54061 aaaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag atgacgagga
    54121 gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca gaaaatccac
    54181 aaaaaatgtc tgcaaagata aaacaaaagg attttcctgc agctggaagc attcatacga
    54241 attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga ttggattaaa
    54301 aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg gttatgattc
    54361 agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac aaggcagaat
    54421 tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta ttggtcatac
    54481 acgatgggca actcacgggc aaccttctga agaaaacgcc catccacata cttcccaaag
    54541 tggacgcttc gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac ttaaaattgc
    54601 ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg ctcatctaat
    54661 tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt tgcgggtcat
    54721 caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta tttacgtcgc
    54781 taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga ttgccagcga
    54841 tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg aagagatggt
    54901 taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg tagaacgttc
    54961 ttcattcgaa gcacaagtag atgcttcaga tatcgagaaa ggtacctatc ctttttatat
    55021 gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa aatacgtaac
    55081 agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg atagcgatcg
    55141 aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta aacagacttt
    55201 ggaaaaattg acacagattc ctgttgaggt tcatttagcc agtgaatttg gctataacac
    55261 acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg aaacagcaga
    55321 cagccgccaa gtgctggtaa aaatcaaccg tctcggctac ccttcactta cgattacaaa
    55381 tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc atgctggacc
    55441 cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac taactttatt
    55501 agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg atatcactca
    55561 tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag attatttaga
    55621 agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg gacgtggtgc
    55681 tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt atatccaggc
    55741 agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg aagaaggaac
    55801 accagtgatc ggaatcatca gtgaagaagt gactggtgcg catactcgag gaaacctgaa
    55861 agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat tagaaaagga
    55921 aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa ccacagtcgt
    55981 accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg ttgacaaacc
    56041 acgtaattta gcgaaatcag tgacagtaga ataataaaag aacaaggacc agtgtcagac
    56101 atctgacact ggtccttgtt cttttatttt ttatgaaaat atctcttaat ccagtccgat
    56161 ttcttctttg actactgccg cgattttatc gacatagtaa ttaactttct catcagtagg
    56221 cgcttctgcc atcactcgaa gtaatggttc tgtaccggaa ggacgaacta ggattcttcc
    56281 ttctgatccc atttcagctt ctgcctcttc aatcactttt ttgattgccg gaacatccat
    56341 cgcaccgttt ttatcactta cgcggatatt cactaatttt tgcggataga ttgttacctc
    56401 tgctgcaagc tctgataatt tcttacctgt ctgtttcatg atattcagta actgaattcc
    56461 tgaaagcatc ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc cagactgctc
    56521 gccgccaaag ttataatcgt ttttgcgcat ttcttctaca acgtaacgat caccaacttg
    56581 tgtaacgaca tctttcaaac cgattccttc caccgctttg tggaatccta agttactcat
    56641 gacagtcgta acgatcgtat cttttttcaa tcgatttttt tctgccaagt atttagcaca
    56701 gataaacatg attttatcac cgtcgacaat gtttccaagt tcatccaccg caataatacg
    56761 atcgccatcg ccatcgaaag ctagtccagc atctgctcct ttttcaacga ccatctcagc
    56821 taaacgttct ggatgggtag agcctacgcc gtcattgata tttaacccgt ctggatttgt
    56881 gcccattgta taaaaatctg tttcaagatc ggcaaacaaa cgattaacac ttgttgaagt
    56941 tgctccgttt gctgcatcga cacaaatggt caaaccggaa aggtcaccgt tgatcgtttg
    57001 tacaaggaat tgagaatatt tcaataagcc ttctggaaat tcttctacag ttcccaatcc
    57061 ttcagctgat ggacgaggca attcatccac ttcagcatct aataatgctt caatttctag
    57121 ctcttggtca tctactagtt taaaaccatc tgcaccgaaa aatttgattc cattatcttg
    57181 tgctggatta tgagaagcag aaatcatgac ccctgcgctt gctttttgca aacgtgtcag
    57241 ataagcaaca cctggtgtag agatcacacc tagctggaac acttcgatac ccaccgataa
    57301 aagtcctgaa atcagcgctt cttctaacat ttgacctgag attcttgtat cccgccctac
    57361 taacacgcgt gggcgttttc cttcttcttc atgctggctt aggacataac ctccgcaccg
    57421 tcccagctta aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc gtacgccgtc
    57481 tgttccaaaa tatttaccca tagttgtaaa attactcctt taacttaatt ttctgaggaa
    57541 tttgttctgc tcatttgtgt tgattcgctt gtttctgatg tttcttctgt acttgtggtc
    57601 gagctgcttg tcccagattc cgaactggaa gaaatggttg ttgtcgatgg ttccgttcct
    57661 gaatcagcag atgaaatact tctactcgta cttgtatttt cttgtgtccg actgctcgtc
    57721 gaagcagagc tactgcttga cgtttgcaac acgggtgtaa tctccacact aactgattcg
    57781 ggcttgatac ttaacccctc ttctactggt atttcaattt tacgagttgt tcgatgattg
    57841 atacctgaga cattgatcgg tacagcaata ctgtcaccga tcgatgcaag caaatcgcta
    57901 gagccgctta cttctgcttc cagttttgaa aaactgaaat catagctttc taccccggca
    57961 gctggtgttc cttgctgtat cccataaagt cgtaccgact tgcttggctt gatcgaatca
    58021 gctctcactg ttacttgcac tggatcagag acgatactta atacttcccc tgctgcattc
    58081 agcgcctgta ttgggacctc actattaatc ccatcatctg tgaccatcga agcgtccaca
    58141 tcagctacta ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat agagactttt
    58201 ttaggactaa cggaagtatc atcaagttca tatccagatg gtgtgaacgt tgaaggaatc
    58261 aacgtttcca catcgaaatc ttttattacc tttttctcaa ccgttactgt aatagtcgat
    58321 ggttcgatct ttgctgtgac agcactgctc aaattttgaa tttttagcgg aacttcatgt
    58381 gttccttcgc ccagcttagt gatatctgct gtgacgcgga acatccgtgt atcttcgttt
    58441 gcctctgcat tcaactgaac tcggtttgca ctggaaagtt tgacagtcac tgcattttca
    58501 tatccatgga tatagtagcg ctttgaatca taagaaatct tgatcggcac ccccgtaact
    58561 gtttgattat acgcttcatt tccagaaagc gtatttggaa cggactggcc atttgcattg
    58621 aagtacagcg caatactgaa cagcaatgca ataaaactgt aaagaatgct tgtccgtttc
    58681 tcttttgaga tcatttgcga tcccctccct tagtcatacc gtctaggaaa gattgaatca
    58741 cgtttttctt gttttttgtt ttctcttcag caattagttc tctacgcaag attgccaaat
    58801 attcttgctg ggataagcca gccatcagtt cattatctaa cgtgatgctg acgtccccgg
    58861 tttcttcaga gacaacgatg gtaatcgcgt cactgacttc gcttataccg acagctgccc
    58921 ggtgtcgagt cccgaattct ttagggatca acatactttc tgatagagga agataggcgc
    58981 ttgctactgc gatctttcct tcttttacaa taactgcacc gtcatgcagc ggtgtattag
    59041 gaataaagat attgatcagc agctccccag tgatatctgc atctaaagca atccctgtct
    59101 caatatactc atctaatcca gtatgacgtt cgatagtgat caacgcaccg atttttcgct
    59161 tggacatata ttggatggct ttgtcaaaag acaagatcat cttctcatct tcctgctgtt
    59221 cgctttttga ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct ctccggattt
    59281 ccggctgaaa aatcacgata gcagctatca ccccgtacgt aatgacttgg ttcatcagcc
    59341 aagacaaggt gtgcagacca atgatttccg ctacgattcg gataacaata aaaatagcta
    59401 cacctttcag aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt ttgtatacaa
    59461 gataccagac aaccagaata tcaagtatgt tcaccaaaaa attcagagag aacaagtcac
    59521 cggaaaacat ttgctgccaa tagtccaaat taaaaagttc cccgatttga aatgacataa
    59581 aaagcctctc ttctcttttg agatttcctc tctagtatat catatgtttt aattttagag
    59641 gctgatatgc tcgtattttt cttgagaaaa acgcataatt ttcaagaaaa ttgcaagtat
    59701 gcgttttatt tttctctgca ataaatattt ttaaacttca gagaattatt cattatattt
    59761 tttctgttat ttttcctttt tttcttttgt agcttgttcc atttttttac gtcgttctgc
    59821 tctggcagct cttctagcag ctcttctttc ttctgcttca aggtccgtcg ttttttcttt
    59881 tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct tgattttttc
    59941 taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtcgt ggacagtctt
    60001 ttcaaataaa gcttttgcaa gatttggatt tgcgttatct agcgaggaaa agcgaacttg
    60061 tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca aagtcattgg
    60121 gttcttgttt ttttcgacca ttgtagggtt atatcgatat aatgaccaat aaccagaaga
    60181 aacagcttct tttgcttctt taagcgattc tttcattccg ccaagcaagc catgtgtgat
    60241 acaaggcgta taggcaataa tcagagaagg accaggatac ttttctgctt cttcaaatgc
    60301 cttgatcgtt tgcatttgat tggcacctga agcaatttgc gccacataaa catttccata
    60361 tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag ccgaaaattt
    60421 agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg aatagacttc
    60481 gttatccaaa acaaggatat tcacgtctgc tccgctggca agtacatgat cgatcccgcc
    60541 aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt tttttacgaa
    60601 tagatccttt ctcttgtata tatcctccag ttcagggaaa attgatcgtt cgctttcgat
    60661 cgcagcttgc agttttgctg ctctttgctg cgttccttca ctctcaaaca tgtgtactaa
    60721 ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt ctttgattga
    60781 acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac caaattcagc
    60841 attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat tggtcgtata
    60901 aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat tcgcgatcat
    60961 cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc cacagcctga
    61021 gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga cagtattctt
    61081 tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat tcacagcttg
    61141 ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg gacaagcctc
    61201 tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt attttacgcc
    61261 gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca tttcctcttc
    61321 atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt cattacacat
    61381 cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct tttccataga
    61441 agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg taccaacaga
    61501 gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat aaccagtgtt
    61561 ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga tcgcgacttg
    61621 atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga tagccattga
    61681 tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct catcaaaagg
    61741 aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc ggcgtcccaa
    61801 accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt tattttcagc
    61861 caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc tccaaagagt
    61921 attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc tatgcaggta
    61981 agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgctg aacgtatcgg
    62041 cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg aatcataata
    62101 aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag ctgatttatt
    62161 tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg tttgcggttc
    62221 tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat ccacgatccc
    62281 tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa aaactgctga
    62341 gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa taacagccgg
    62401 gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt ctccacctgc
    62461 ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag gcaacttttg
    62521 cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac ctgttttccg
    62581 tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg atcccataga
    62641 aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat aagaagttcc
    62701 tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt tatcataata
    62761 ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg taccactaac
    62821 agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct cctgattgat
    62881 cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt gactcgtacg
    62941 gaatccatca aagaaattca aaaaagggac ttctccttca atagaagcaa gatgggcaac
    63001 aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa atcctgtttg
    63061 tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg tcgtcactgc
    63121 ccgactagca acgtggaaaa cacttggcag tagttcccca gcaatcttgt acatattcgg
    63181 tatcatcaaa agcagccctt gtgaagcggt atatgtagtt gtcagtgcac cagttttcaa
    63241 tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta cttttactgg
    63301 ttgaccgaat acgttttctt ttcctgctgc tgaccattga tctactaatt cagccatggt
    63361 cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag aaatatatgc
    63421 ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc cttgttcata
    63481 ttctctctta ttatctgttc cattataacg taaaacatgc catttttttc ttttatttat
    63541 aaaaatattt tacatgtatc tatttgaatt ggtcatctaa tttttagaca aacagtctaa
    63601 atcgttgcct ttttttgtca gacatgtata aactataagc gaaagaggtg ttttaacatg
    63661 gatatatcag tgattgatgc aacgaaaaca aatacacaaa aaggaatcct ttatggttca
    63721 tcgaatgctc ctaaaaaaat ggtcgaattc atcaacttag cttgtccgta ttgccgacaa
    63781 tggtttgaag aatcttatga gctattagaa gaagcagtcc aaagcgggca attgcagcga
    63841 gtgatcaaat tgttcgataa agaaaaagaa agcttacagc gagggaacgt gatgcaccgg
    63901 tatctcacaa tcagcgatgg ccaaaaagcg atcaaagaga tcaaacaaat attcgatact
    63961 caagacgagt ggaaacatct ttctctccaa gaagtagcgg attttgcagt agataaacta
    64021 aagctgacag aacaaaaaga cgaacagctg agccaagctg tcatcaacga agctgagcaa
    64081 gcacatattc gatttgttcc aactgtcatc ttgggtaaag aaatctttga tgaatctatt
    64141 tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataa
//
LOCUS       DACBZN010000005        63116 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.005, whole genome shotgun sequence.
ACCESSION   DACBZN010000005 DACBZN010000000
VERSION     DACBZN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.005"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            107..970
                     /locus_tag="IVD64_000307"
     CDS             107..970
                     /locus_tag="IVD64_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6147584.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IVD64_000308"
     CDS             complement(1099..2721)
                     /locus_tag="IVD64_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6147585.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IVD64_000309"
     CDS             2970..3491
                     /locus_tag="IVD64_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147586.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IVD64_000310"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IVD64_000310"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP6147587.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IVD64_000311"
     CDS             5572..6390
                     /locus_tag="IVD64_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP6147588.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IVD64_000312"
     CDS             complement(6493..6690)
                     /locus_tag="IVD64_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147589.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IVD64_000313"
     CDS             6859..7437
                     /locus_tag="IVD64_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6147590.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IVD64_000314"
     CDS             complement(8482..8985)
                     /locus_tag="IVD64_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6147591.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="IVD64_000315"
     CDS             9344..10369
                     /locus_tag="IVD64_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6147592.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10716..11594
                     /locus_tag="IVD64_000316"
     CDS             10716..11594
                     /locus_tag="IVD64_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147593.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12070..12186
                     /locus_tag="IVD64_000317"
     CDS             12070..12186
                     /locus_tag="IVD64_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6147594.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13114..13452
                     /locus_tag="IVD64_000318"
     CDS             13114..13452
                     /locus_tag="IVD64_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147595.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13546..13776
                     /locus_tag="IVD64_000319"
     CDS             13546..13776
                     /locus_tag="IVD64_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147596.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14176..14547
                     /locus_tag="IVD64_000320"
     CDS             14176..14547
                     /locus_tag="IVD64_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP6147597.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14821..15513)
                     /locus_tag="IVD64_000321"
     CDS             complement(14821..15513)
                     /locus_tag="IVD64_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6147598.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15516..16301)
                     /locus_tag="IVD64_000322"
     CDS             complement(15516..16301)
                     /locus_tag="IVD64_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6147599.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16538..17938
                     /gene="lacG"
                     /locus_tag="IVD64_000323"
     CDS             16538..17938
                     /gene="lacG"
                     /locus_tag="IVD64_000323"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6147600.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17963..18280
                     /locus_tag="IVD64_000324"
     CDS             17963..18280
                     /locus_tag="IVD64_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6147601.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18293..19966
                     /locus_tag="IVD64_000325"
     CDS             18293..19966
                     /locus_tag="IVD64_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6147602.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19980..21611
                     /locus_tag="IVD64_000326"
     CDS             19980..21611
                     /locus_tag="IVD64_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP6147603.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22483..23673
                     /locus_tag="IVD64_000327"
     CDS             22483..23673
                     /locus_tag="IVD64_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP6147604.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23807..25996
                     /locus_tag="IVD64_000328"
     CDS             23807..25996
                     /locus_tag="IVD64_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6147605.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            25993..27786
                     /locus_tag="IVD64_000329"
     CDS             25993..27786
                     /locus_tag="IVD64_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6147606.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            27986..28300
                     /locus_tag="IVD64_000330"
     CDS             27986..28300
                     /locus_tag="IVD64_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6147607.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28321..28698
                     /locus_tag="IVD64_000331"
     CDS             28321..28698
                     /locus_tag="IVD64_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6147608.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28708..29481
                     /locus_tag="IVD64_000332"
     CDS             28708..29481
                     /locus_tag="IVD64_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147609.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29503..30906
                     /locus_tag="IVD64_000333"
     CDS             29503..30906
                     /locus_tag="IVD64_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6147610.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31088..32272
                     /locus_tag="IVD64_000334"
     CDS             31088..32272
                     /locus_tag="IVD64_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6147611.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32269..32559
                     /locus_tag="IVD64_000335"
     CDS             32269..32559
                     /locus_tag="IVD64_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147612.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32556..32777
                     /locus_tag="IVD64_000336"
     CDS             32556..32777
                     /locus_tag="IVD64_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147613.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            32819..33598
                     /locus_tag="IVD64_000337"
     CDS             32819..33598
                     /locus_tag="IVD64_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP6147614.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            33665..34159
                     /locus_tag="IVD64_000338"
     CDS             33665..34159
                     /locus_tag="IVD64_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6147615.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34215..34853
                     /locus_tag="IVD64_000339"
     CDS             34215..34853
                     /locus_tag="IVD64_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147616.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            34925..35320
                     /locus_tag="IVD64_000340"
     CDS             34925..35320
                     /locus_tag="IVD64_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6147617.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35304..37757
                     /locus_tag="IVD64_000341"
     CDS             35304..37757
                     /locus_tag="IVD64_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6147618.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            37754..39781
                     /locus_tag="IVD64_000342"
     CDS             37754..39781
                     /locus_tag="IVD64_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147619.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            39778..40800
                     /locus_tag="IVD64_000343"
     CDS             39778..40800
                     /locus_tag="IVD64_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6147620.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            40817..41746
                     /locus_tag="IVD64_000344"
     CDS             40817..41746
                     /locus_tag="IVD64_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6147621.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            41991..42107
                     /locus_tag="IVD64_000345"
     CDS             41991..42107
                     /locus_tag="IVD64_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6147622.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            42123..44042
                     /gene="tet(M)"
                     /locus_tag="IVD64_000346"
     CDS             42123..44042
                     /gene="tet(M)"
                     /locus_tag="IVD64_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6147623.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            44140..44328
                     /locus_tag="IVD64_000347"
     CDS             44140..44328
                     /locus_tag="IVD64_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6147624.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(44386..44739)
                     /locus_tag="IVD64_000348"
     CDS             complement(44386..44739)
                     /locus_tag="IVD64_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6147625.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            45270..45692
                     /locus_tag="IVD64_000349"
     CDS             45270..45692
                     /locus_tag="IVD64_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6147626.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            45689..45919
                     /locus_tag="IVD64_000350"
     CDS             45689..45919
                     /locus_tag="IVD64_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6147627.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            46417..46617
                     /locus_tag="IVD64_000351"
     CDS             46417..46617
                     /locus_tag="IVD64_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6147628.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            46644..47837
                     /locus_tag="IVD64_000352"
     CDS             46644..47837
                     /locus_tag="IVD64_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6147629.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(47905..49470)
                     /gene="guaA"
                     /locus_tag="IVD64_000353"
     CDS             complement(47905..49470)
                     /gene="guaA"
                     /locus_tag="IVD64_000353"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6147630.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(49529..50452)
                     /locus_tag="IVD64_000354"
     CDS             complement(49529..50452)
                     /locus_tag="IVD64_000354"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6147631.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            50648..51253
                     /locus_tag="IVD64_000355"
     CDS             50648..51253
                     /locus_tag="IVD64_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6147632.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            51561..52577
                     /locus_tag="IVD64_000356"
     CDS             51561..52577
                     /locus_tag="IVD64_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6147633.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            52718..54019
                     /locus_tag="IVD64_000357"
     CDS             52718..54019
                     /locus_tag="IVD64_000357"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6147634.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            54065..54727
                     /gene="deoC"
                     /locus_tag="IVD64_000358"
     CDS             54065..54727
                     /gene="deoC"
                     /locus_tag="IVD64_000358"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6147635.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            54748..55146
                     /locus_tag="IVD64_000359"
     CDS             54748..55146
                     /locus_tag="IVD64_000359"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6147636.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            55212..56294
                     /locus_tag="IVD64_000360"
     CDS             55212..56294
                     /locus_tag="IVD64_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6147637.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            56506..58068
                     /locus_tag="IVD64_000361"
     CDS             56506..58068
                     /locus_tag="IVD64_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147638.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            58061..59194
                     /locus_tag="IVD64_000362"
     CDS             58061..59194
                     /locus_tag="IVD64_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147639.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            59194..60150
                     /locus_tag="IVD64_000363"
     CDS             59194..60150
                     /locus_tag="IVD64_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147640.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            60249..61415
                     /gene="deoB"
                     /locus_tag="IVD64_000364"
     CDS             60249..61415
                     /gene="deoB"
                     /locus_tag="IVD64_000364"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6147641.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            61439..62257
                     /locus_tag="IVD64_000365"
     CDS             61439..62257
                     /locus_tag="IVD64_000365"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6147642.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            62274..62981
                     /gene="deoD"
                     /locus_tag="IVD64_000366"
     CDS             62274..62981
                     /gene="deoD"
                     /locus_tag="IVD64_000366"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6147643.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        20559 a        10701 c        12828 g        19028 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tatatttatt ctatcaaaaa attttgttta
    10561 tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga
    10621 tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat
    10681 accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg
    10741 gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc
    10801 ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg
    10861 agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag
    10921 acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc
    10981 aaagttggaa aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa
    11041 tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg
    11101 accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg
    11161 agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt
    11221 gcctttctct tcatattcct tccgatgcag atttatctca gaataagata agagatagtt
    11281 acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt
    11341 acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa
    11401 tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag
    11461 tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt
    11521 tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta
    11581 ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa
    11641 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
    11701 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    11761 cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac
    11821 gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa gagggccttt
    11881 tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta
    11941 gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca
    12001 caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg
    12061 gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat
    12121 atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa
    12181 aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc
    12241 aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca
    12301 tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat tccagttata
    12361 tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt
    12421 tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac
    12481 ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg
    12541 ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac
    12601 cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc
    12661 atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa
    12721 ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag
    12781 aataacatca aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg
    12841 gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa
    12901 gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt
    12961 gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga
    13021 ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt
    13081 attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa
    13141 catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg
    13201 gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat
    13261 atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta
    13321 gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt
    13381 gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata
    13441 ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa aatttactag
    13501 atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag
    13561 ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg
    13621 aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa
    13681 ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta
    13741 gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt
    13801 tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat
    13861 gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag taagtagcta
    13921 cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt tcttaattag
    13981 cttattgatt ctataatatt tttgatttat ataggaaatt aaaaatatta tagaatcaac
    14041 actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga aatttctcct
    14101 ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag
    14161 tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa
    14221 tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc cattatttct
    14281 tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga taaagacaaa
    14341 attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga tcttaagttt
    14401 aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa agatacggaa
    14461 acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac tcagcttggt
    14521 ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa agtatatcaa
    14581 aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa cactattttt
    14641 aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa aaagtcaaag
    14701 atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt ttcactggca
    14761 gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta gttgaatcgt
    14821 ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa aatctttgat
    14881 ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac cgaatggatc
    14941 aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg agtaagcatc
    15001 ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt taagaaaaat
    15061 atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa
    15121 ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga cagtagccgt
    15181 ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt caagcccttt
    15241 tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc tttctttttc
    15301 ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat gtttcaaaat
    15361 ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata aaccatcttt
    15421 tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt ccaaaaacat
    15481 tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt ggtattttcg
    15541 gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac ctcccgcgag
    15601 atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac ataaaaatca
    15661 tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt atttagagcg
    15721 atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca
    15781 tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt
    15841 ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat ttctggtcca
    15901 tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg aattttcttg
    15961 ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa aatcgtttct
    16021 ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg aatctgcaga
    16081 gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact ttgagcccct
    16141 ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct gcgaacggtc
    16201 atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc ttttttattg
    16261 accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac ctctaattta
    16321 aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa gtaaacattt
    16381 agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt tttatgatta
    16441 tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat cagttagaaa
    16501 gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac cagaagattt
    16561 tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg aaggtggcaa
    16621 aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc ccgatccggc
    16681 aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa
    16741 tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg ggaaaccaaa
    16801 tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac
    16861 gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc atggagattt
    16921 tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt ttcatgaatt
    16981 caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa ccaatcaata
    17041 tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg tcgcctgtct
    17101 tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc
    17161 gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag acgcgccgga
    17221 agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat tagatgctac
    17281 ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat gtgaagcaaa
    17341 tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg
    17401 taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt acgatggaga
    17461 aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata aagtgaaggg
    17521 aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact ggctgattta
    17581 tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac attacaacaa
    17641 aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg gcattattat
    17701 ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca aggcgataac
    17761 agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt tctcatggac
    17821 aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc agaagcgtta
    17881 cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga ttatttaata
    17941 agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg gttttgagat
    18001 cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag aagtaagaca
    18061 aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt taacccttgc
    18121 acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga tggagatggg
    18181 attcattttt atccatgggc aggatcattt aatgaccact ttattgctga gagatctgat
    18241 tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg ctatggatgg
    18301 aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac gtaatagtta
    18361 tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat tttcaagcat
    18421 tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg aatctgtctc
    18481 cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac tcgtttcagc
    18541 aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat
    18601 caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc
    18661 gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg cttcctttgt
    18721 cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata ttaccattaa
    18781 aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt ttcctttctc
    18841 tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct tccaaatctc
    18901 atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg acgggtactt
    18961 aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg ttcatggacc
    19021 gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca
    19081 attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag ggaattttgt
    19141 aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc tactctttgc
    19201 taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa gtttcgctgt
    19261 caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt ttattccatt
    19321 tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg tattgggcat
    19381 gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt tggttctagg
    19441 aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg ttgacttcct
    19501 gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag aagcacaaaa
    19561 agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt
    19621 ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc
    19681 tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa
    19741 agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc tgcaggattt
    19801 tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca aagaagacac
    19861 cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg gattaacgag
    19921 agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga ggagccataa
    19981 tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta ggggctttgg
    20041 gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga gcagatattt
    20101 cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat atttcaaaag
    20161 atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt ttgtggcagg
    20221 atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg gaaacgacga
    20281 ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat tttcactaca
    20341 gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa ggtctgacct
    20401 ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc
    20461 agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc ggaatgctct
    20521 ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg gctgcttttt
    20581 tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca gtaatgctcc
    20641 acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa atcacgagtc
    20701 gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac ggttcaatga
    20761 atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa gtgatcgtag
    20821 ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat gcttttggtg
    20881 aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca gagtatttgc
    20941 tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc ttcttctatt
    21001 gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt atgaattatc
    21061 tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt gattttgctg
    21121 gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata aatctagtta
    21181 aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg caaacctggt
    21241 ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt tttggggact
    21301 acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa aatgttagtg
    21361 gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga gagtttgata
    21421 ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg gttgttaaaa
    21481 caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt gtttgtgaaa
    21541 ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt gtttcattgg
    21601 tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc agccacatcg
    21661 ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata accagaaacg
    21721 atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca ctttgtatta
    21781 tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca
    21841 gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga atatgatttg
    21901 agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga gaacttcttt
    21961 ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag ggaaccatct
    22021 tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat agggattccc
    22081 cctccatatt atgttggctc tttattccca ctctgttttc aacgtaaagt gtataaaaca
    22141 gaatgcgata ctatcaaggg agttaaaaca atatacagaa cagtaaaaca ctataaataa
    22201 aaataaaagt gcaataaaag tgcaattttt gcacctaatc atactctgtt tatatatgaa
    22261 aactagattc tgaatccaat atttggttaa cgagaggcgt tcgaatttga acgtctttta
    22321 tatattactt tcaaataaat atgaggtaga atactatcta ttttcttttt aacccaattg
    22381 ttataatgga gagtgtaaaa tattttgtgt aaatagaaaa aaggaagtcc cttctgtaga
    22441 atagagttac cacaacacat tcacagaaga gaggacttcc ctatgaacga ttttactaca
    22501 gaaattctaa agactctagc gaacaaaggc gatttgaatg aattattccg tgtccatttg
    22561 gagaaagcag tcaatacgct tctcaaaacg gagttaacgg ctttcctaga ttacgaaaag
    22621 tatgatcgca ttggttttaa cacgggtaat tctcgtaacg gctcctatga ccgtacggtc
    22681 aagaccgagt atggggaact tcatctccag attccgcgcg accgcaatgg tgagttcaag
    22741 caacagactg ttcctgctta tagacggacg aatgacacgt tagaggagac cgtcattcac
    22801 ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa aatgtatggg
    22861 cattactaca cgccccaaac catgtccaat ataacaaaat catttacaga agaggtaacg
    22921 gcgtttaatg ggcgggagct tcatgaccgt tatgctgcta tttatatgga cgcaacgtat
    22981 attccgttaa agcggaaaac cgtcgccaag gaagctattc atatcgcagt tggcattcgc
    23041 ccggacggat caaaggaagt attgagctat gcgattgcac cgactgaatc catcacgatt
    23101 tgggaggaaa ttttattgga ccttcaagag cgcggtttga aaaatgtcct cctgttcatc
    23161 acggatggct taaaggggat ggtaggagcg attagtcggt tctatcccaa agctcgtttt
    23221 caacattgtt gtgtacacgt ttcccgtaat atcagtcata aagtgcgtgt cgatgatcgt
    23281 aaggaagtct gtgatgattt taaaatggtg tatcaagcct catctaaaga ggtggcgttg
    23341 gaagcacgtg gtacttttgc ggaaaaatgg aaaaccagct atccaaaagt ggttgaatcg
    23401 attctttcga acgatcactt gctcactttc tatgatttcc ccttggccat acgcaagagt
    23461 atttattcta cgaacttgat tgaatccttt aataaacaaa tcaagaaata cagccaccgc
    23521 aaggaacagt tccaaaatga agagtcgatg gaacgtttct tagtctcgtc ttttgatact
    23581 tacaaccaaa aattcctagg tcgcagtcat aaaggtttcc aacaggccga aggcgagctc
    23641 gaacaaatgc taagccaact gattgagaat tagaaacaat ctgcagtagg gaagaatcat
    23701 ttacacaaaa ttattgacgc tcccttataa tgagaacttt tactaacata atttatgata
    23761 aacttactat ataatcatat taattaaata gttaggagat ctgcttatgt ttatatctac
    23821 tcttcaaatt cgaaatttta gaaattatga atccgaaatc tttcgattcg ataatgagac
    23881 aaatactatt ataggtgaga atgattcagg gaaaaccaat gctcttactg cattgagaat
    23941 cttattagat gatagttatt actatagtag taaaacacta aaagaatcag atttttttca
    24001 tggaattcaa aatgggtggc aagggcattg gattattatc tctgcgacat ttgaaggaat
    24061 cagcgaagaa gaatttgaca atgagatttg tgcttccatt tctttaaatt ctgaaagtca
    24121 aacaatctta gaagaattga tttcaaatgc agataaaggt gtaggtagta tttctctatt
    24181 tattagacct aacaaagcta ttagaaagca attatttgat atttccactg aattagactc
    24241 tcatcaattt aacgaattta gaaattctat tcgtctatcc gactatgaat tttactacac
    24301 atctaaatca aatctaaatt tctgtatcaa tgaaaattat gataaattgg taggaagact
    24361 caatgacaat atagctagta atcctgaaga agacgatgaa gctctgttgg gctcaagaat
    24421 tgacatgtca gatattttca agcatatttc ggttgtttat gttgacgctc ttcgagatgt
    24481 tcttagagaa atgaaaaaca atcgaaaccc agtcaaaaga atcatggaaa caattgaatc
    24541 aaaaatatca tcagataatg ttgagtcatt aaaaacaatt atacagcaat taaatgagac
    24601 tattaccagt gttcctgaaa ttaggaaaat tggcgaaaat attaatcggc aactaaatag
    24661 cataattggt tcagtgtatt ctcctaatct tcttctttcc tcaactatgt cagatgatat
    24721 gggttcttta gcaaaatttc tttcaatgaa gccagaacaa aatatcgact tagacttact
    24781 agggttagga catctaaata tgatttattt agctttgaaa atagtcgaat ttgaagcgtg
    24841 tcgttcaaga gaactactta atattatgtt gattgaagaa ccagaagctc atattcatca
    24901 tcatattcaa aaaactctgt tcgaaggatt aaatcttcaa aaaaactata ctcagattct
    24961 catgaccaca cattctgtac atttagctga agtgtctgaa atatctcgga tgaatgtatt
    25021 aaaatcgttt aatggcaaat ctatcgttat gaatccagca aataatctga actcatttgc
    25081 taaagaaaag ttaaaaaaga acaatctaaa tctaataaac gcagtagaga gatatttaga
    25141 tgttaaaaga aatggattgc ttttttctaa aggtgttatc ttagttgaag gagatgctga
    25201 agaaatactt attcctcaaa tagtaaaaaa attatttggt ataaacttag acgaacttgg
    25261 aataggtttg attaatgttg gtagtacttc gtttgaatat atcgctagct tgtttgacga
    25321 tgatagaata cagagaaagt gtgctattgt aactgattta gatgaacagg caatcccatc
    25381 agaacatcga ttgtataaaa gcactgcaca agagaaaggt caagcaagaa aagaaaaatt
    25441 atcctccctt tttaatgaaa acaattgggt taattgtttt tatgctaata caacatttga
    25501 aattgagttt ttagatgtga ataaagaaaa tattctaact tatgtcagtc caattataga
    25561 tgacgcattc aaagataaaa aaacaataac tgcttataaa gaagaaatac aaagtgaaca
    25621 atatttagaa agaaatttag cgatgttaaa actagccaat catgttggta aggggtggtt
    25681 tgcaattgag ttaggtgaaa aaattgactt tttaatcaaa attcctaagt atattcttaa
    25741 tgctattgct tttgcatgtc aggaaatagt agatctctca atttataaaa aaattctact
    25801 ccatacaatc gacttgtacg aggaaactac agaattgtca gctttatcag ctagtttaaa
    25861 aacaagtgat acagctgatt tgaatatagc aataatttct ttattagagt actttggtaa
    25921 agatgaggat gttgtgtatc taatcaatca gatagaaaat tacacaaaaa tactttatgc
    25981 tgaggtttca aaatgacaaa tatcttcgaa aaaatcgtaa atgatttaaa tttagaacaa
    26041 aaagatgttg tggaaagtga agataacctt tatgtaaatg catgtcctgg gagtgggaaa
    26101 acaagggtac ttacaaggaa aattgcttac caagcaatta aacacgatgg atcactaaaa
    26161 agaataattg ctatcacata cactaataga gctgctgaag aaattaagga acgcctaagt
    26221 ttacttagta tagatgatga tattaatatt tgggtgggga caatacatca gttttgtctt
    26281 gaatttatta tttatccttt taaaatgaat ctctcaagag tatcaaaagg atttacaatt
    26341 attgatgatt atactcaaag agcttacgtc agccaaatta ataatatgct gaatttaaat
    26401 ctcaaaacgt atgaactgaa caaaattgat ttgtcacttt ctcatgattt taaaattaat
    26461 gaagttcagt atactcaagt ttctgaaatg tatcacacta tgttggaaaa aaataaagaa
    26521 attgattttg atatgatttt actagtagcg tataaaattt taactgtaaa cagtaacgtt
    26581 gcacgtaata ttgctagtaa tataagggcg atttatgtgg atgaatttca agatactaga
    26641 gaattacaat acaatgtaat agctaaatta cttcaaaaca accctcaaat tcagtcaatg
    26701 tttgttggag atattgacca agcaatttat ggatcattag atggtatagc taaatcagtt
    26761 gaagaactag aaatgctaac tagtcacact tttcaatcaa agacgctaca tggctgttac
    26821 aggtctaatc agagattagt agacttctac tctaattttc agagttgtcc ttacgagatt
    26881 gaaagcagag ggaataataa aaacgaccgt ggttttataa gttatgattg caaagttact
    26941 aaggaggatt tacccaacat aataaaaaat ataataaagg aaaagattga aagcggcatt
    27001 catgaaaaag atatttgtgt acttgctcct caacactatc ctctgttttc actgggtgag
    27061 tatttaaaga aagaattacc gtattgcaat tttgatgcta taaatatatc accaattaag
    27121 gtaaataatc atagtttatt tttcaagtta gcaattttgt actttactga atcaggtgaa
    27181 aaggttcgaa gaagaaagct aattgcaaac gatattttaa ttatattaaa aaatgaattt
    27241 ggaatagaag ttaaagatta ttttattgac ttagatttat tacagctaat aaacagtgtg
    27301 aaaagaatgt atagtggaga cgacaacggt gttgatttat acaaatttgt taccaaacat
    27361 atatttgtta atcttaatat tgacgagaaa aattacccgt ctatttttaa gcactatcta
    27421 ttttttatta acgaagtaga agatcggata aaaaataatt cattatctag tagtgtatct
    27481 agttttaaga agatgtttaa atctcgagat ggtatcacac ttactacagc acataaagtt
    27541 aaaggaaatg agtatgatac tgttatcgct attaatcttc taaacgggaa aattcctcat
    27601 tggaatgaaa tttttaatgt ttctgataaa ggaattagtt cagcgagaaa actactatac
    27661 gtaatttgtt caagagcaaa aaataattta tttcttttct cagaggtggg ttattttacg
    27721 agtaaaggag acttgcttta tcccactcaa ttattagata aaatagaatt tgattataat
    27781 atttaaaata cctaggtagt cgttgtttta aatatagtag gaatcggacg ctcagtacgg
    27841 agcgtctatt tttttaccca attttgaaag gaagtgaact tatgaaaata aaaaatcaag
    27901 aatcaaaagg tcgctctccc ccctttaaaa ccatcacaca aataagattc attcaaacat
    27961 aaaaacagaa aggataggta aaaatatgga acttaaatat gtcattccca atatggaaaa
    28021 aacatttgga caattagaat atgctggtga agggaacatt gaacaacgtc gaataaatgg
    28081 cagaccaacc attctctctc gtagttacaa tttatactca acgattcaac gagcagatga
    28141 tattgttgtc attcttccct ccgaagctgg cgagaaacat tttgaatccg acgaaaaagt
    28201 caaactcatc aacccacata ttaccgcaga aggttataaa attggcaatc gaggatttac
    28261 caattacatt atgcacgcag acgatatggt gaaagcataa gaaaggaaag gtgaaaaaat
    28321 atgagattag cagaaggtat tgtgattgaa aaagaccaga catttggcac attgaaattt
    28381 tccgccttgc gtcgtgaggt tcgtatccag aatgaagatg gtacggtttc cgaagaaatt
    28441 aaagaacgta cctatgactt aaaatcaaaa ggacaaggac gcatgataca agtcagtatc
    28501 cccgcttctg ttcctttgaa agaatttgac tacaacgcag aagtggaatt aatcaatcct
    28561 gtggcagata ccgtagcgac ggctactttt cgaggggcag acgttgattg gtacgtgaaa
    28621 gcagacgatc ttgtattaaa aggacagaca aaacctcatc agaaatataa aaaagaagcg
    28681 tccgaaagtg aaaaataggt gttgcttatg acaaacacac cattgatttt gaaagaaatt
    28741 ccgaaggatg aagccatttc ttttattcgg caataccatt attcaaagat actccctaga
    28801 ttgtgtaaat actttctagg gattttttca gaagaaaaat tattaggcgt tgtggaatta
    28861 ggctggggta cgcaacccct acaaaccata cgcaaactct ttcccgacag ttcccttcaa
    28921 accacagatt atttagaaat tggaaagatg tgttttcttc cagaaatgaa tcaaaccaat
    28981 tattttggca gtcaagctct ttcagctcta attaagtggt tgaaagaaca tacggactgt
    29041 cattttttat atacactggc agatggaatt gagggaaaat gtggctatgt ctatcaagcg
    29101 tccaacttct tttactgcgg gtatttcaag acaagtgtct atcgtgataa gcagtcatgg
    29161 gaaaagatac accctcgaag tgctcgcctt ttattagagg aaaatgctcg ctttgaacag
    29221 gtagagaaaa agcattggct ttcccaggca ttttgcgaat acaaagggat tgagaaaatc
    29281 aatggacgca tgtttcgcta tctctatccg ttgacaaaag aagcgaaaaa actgttagga
    29341 cacactctct atcgacgtca ttattatccg aaagaaaaaa atttacgctt tgaaaagcga
    29401 atcgcttatc agaaatatga agccatttct caaccaactt ttgataaaca ggctcgtatc
    29461 tacaatacac aattattttg ataggtggaa aggaggattt ttatgccatt gcttcatgac
    29521 agaggagagc gaatcaaagc caacgataag cacttggtct atcattttgt actaatttca
    29581 ttgctggtag tttttctagt cgtcctactc ccttttcatt ggagagcgtt acaatcggtg
    29641 aactggcaag cagttcagtt agatacattt ttctatcaac ttcatacgcc atacttttgg
    29701 agcagtattc tcactgctct ttttgtatgc ggacttatca gctggcttta ttatcactat
    29761 cgtatagatt ctataaaaca gttagagcac cgtcaaaagt tagctcgtat gattttagaa
    29821 aatcattggt atgaagccga agaaactcaa tcagagggat tctttaaaga tttacccagc
    29881 aagaaaacca aacaacgaat ccgccatttt cctaaaatat attatcgaat gaaagatgga
    29941 ctaatccata ttcatgtaga aatcacctta gggaaatatc aagatcagct tttgcaccta
    30001 gaaaacaaac tagaaacagg attgtattgt gaattagtgg aaaaaacgtt gaaagattct
    30061 tatgtagagt atgtcctact ttatgatact attggcaatc gaattggcat tgaagacgtg
    30121 gtagctcaag atggacgtgt caaattgatg gaatcggtct attgggagtt cgattctctc
    30181 cctcacatgt taattgctgg gggcactggt agtggaaaat cctattttat tctcacattg
    30241 attgaatctc tcctgcatac aaatgcaaag ctttatattc ttgaccctaa aaatgctgat
    30301 ttggcagact taggggcggt catgaataat gtctattacc gtaaagaaga tatgttggca
    30361 tgtatcaatc aattttacga agatatgatt gatcgcagtg aaaccatgaa acaacaccct
    30421 aactataaaa caggagaaaa ttatgcttat cttggtttgt ctgccaattt tcttattttt
    30481 gatgaatatg tggcaatgat ggatatgcta ggtcgtgaaa gtaccaccgt tattcataaa
    30541 ctcaaacaaa ttgtaatgtt aggacgacaa gcaggcttct ttttaattct tgcctgtcaa
    30601 cgtccagacg caaaatattt tagtgatggg attcgggaca actttaactt ccgtgtggct
    30661 ttgggtcgta tgtctgaaat gggctttggt atgatgttcg gaagcgatac acaaaaacaa
    30721 tttttcttaa aacccatcaa gggaagaggt tacgtggata caggaaaaag tgtgattagt
    30781 gagttttata caccccttgt accaaaaagg tacgatttct taggagaaat aggaaaagtc
    30841 attcaaaaaa agcaatctga gcctgtaccg cacgaagtga aaggtacagg ttcagacttg
    30901 ctttgattgg tgtggcgtta gccacgccaa tcctttagcc ccctctatct aacagggggg
    30961 cacaaataca actgaaaaca ggcaattctc atcaaccaat gttgatggta caaaggattg
    31021 cggaaaatat gaaaggtgta aactcaaggg tcatagttta catctttttt agattggagg
    31081 tttgcgaatg gaaaacgaaa aaatttggat taaggaattg aaagaaaagc gactggctta
    31141 tggcgtttct caaaataaac tggcggttgc ttctcatatt accagacctt acctatcgga
    31201 tattgaaaca ggcaaggcag tccctactca acaggtgaaa gaagatttac tgaacgcttt
    31261 ggaacgcttc aatcccgata acccattgga gatgttattc gactatgtac ggattcgatt
    31321 ccctaccaat gatgtggctc aaattatcgg tgaggtttta cgtttgaata tggattatat
    31381 gctacacgaa gactttggct attattctta tcccgaacac tatcgtttcg gtgatattgt
    31441 tgtcctagtc tctcatgatg taagtaaagg tgttttatta gaactgaaag gaaaaggctg
    31501 tcggcaattt gaaaactttc tattggctca acatcggagc tggtatgatt tctttcaaga
    31561 ttgcatggaa cataaaggta ttttcaaacg cttggattta gctatcaacg ataaaacagg
    31621 gattttgaat attccagatt tggcaaagaa atgtaaacag gaagaatgca tttccgtctt
    31681 tcgaagcttt aaaaattatc gcagtggtga attggttcac cgtgatgaaa aaccatacat
    31741 gggaaacaca ctttatattg gctcattaaa aagtgaagtc tatttctgta tatatgagaa
    31801 agattatgag caatacgtga aaaatgatgt tcccttagaa gacgcagaag taaagaatcg
    31861 gtttgaaatt cggttaaaaa atgaccgagc cacccaagcc atgaaagatt tattggcaca
    31921 tcaacaggcg gaaaaaacag cctttgaaat tatcaatcgc tatatccgct ttgcagataa
    31981 agacgacact aaacgtcgga gtgattggaa aactaatgaa cgttgggaat ggtttatcgg
    32041 taaaaatcgt ggagcattac gcctaaccac acagccagag ccttattctt ttgaacgaac
    32101 attgaactgg ttacatcatc aagtcgcccc tacattaaaa atcgcaagca ttttagatgt
    32161 tctcaacggt acaaccatta tctccaccat gattcaggaa gccaaactga cagaaaaaca
    32221 tgagaaatta attgaacaac aacatctagc catggaggat ttgattacat gagcctttta
    32281 acaaaagaat taaagaagtt agggtttcag tgtggcattg agtttcaagc gtatatacaa
    32341 aatacaggga aatatacgtc tctcattatt gagggaaaac ggcaagctgg tgataccatc
    32401 tatacctatg atttttacaa ggtgagattt tataataatt ataccaatcg agtaaccgtc
    32461 tatggagagc atttaacccc ttttcaatta ttaaggcgtg taaaaagcta tatctattat
    32521 cgagaaaaat atcttaagga aaggaggaca atcacatgaa ttttgggcag aatttgtaca
    32581 actggttctt ggataatgca cagtcattgg tattgttagc gattgttgtc attggactgt
    32641 ttttaggttt taaacgagaa ttttcaaaac tcattggctt cttgattatt gctctaatcg
    32701 ccgtcggact tgtgttcaat gcagctggcg tgaaagatgt gttattaaac ttatttaacc
    32761 gaatcattgg tgcttaacag atatttttca tgataaaata ggaagtatat attttgttat
    32821 gaaaggattt atgaatatgt ttacaaatga tcaatttaaa tctcttttaa ttaaaacatt
    32881 agaaagaaag agctcttcaa attattatga aggaggtaac gagattgttt ctaaaatgaa
    32941 aatatcagag gtaactttag atgagactga cgattttact tattataaag gatataaaaa
    33001 gggctggaat acgctttgca catatcttaa aataagagtt ccttttgatg atttagactt
    33061 ttttgaaagt cataaagatt tgattaccca gacagcaagc tccatatatg ataaacaagg
    33121 tgataatgta ttagtggata ctattttagt accacttcca gagaattacg aagtaattaa
    33181 ctttagtcag ttgaaaatta gtgatgtggt ttcacaagct attgaagacg ctgagtcttt
    33241 tatgagtaat ggtgaatatc aacgtgcctt tgacagagtt cacactgctt ttcatggata
    33301 tttaattgaa atactaaaaa aatatgaaat tacagttcct agagacgaaa atctatctaa
    33361 attatatagt aggatacaac aattaattga aaaagaaatt caacctactg aacttgctga
    33421 tatagtgaag acaacgataa ggtcatcaaa tggaatgatt agctctctta atgaagcaag
    33481 aaataggcat tcacttgcac accctaatac aaatattatt ggaaagagag aagcaaaact
    33541 aataataggt atttcaagta cagttacaga ttatattagc ggataccttg ataagtagcg
    33601 tcattaactg attgctactt tttttatgac tatttttata aaaaggatgt gataagaatt
    33661 ggaaatgaaa gtctatgtgg caaatttagg acggtataat gaaggtgaat tagtcggagc
    33721 atggtttact ccaccaattg atgaggaaga aatggcagaa agaatcggtt taaatgagga
    33781 ctacgaagaa tatgcaatac atgattttga gcttcctttt gatgtggacg aatacacgcc
    33841 gattagtgaa atcaaccgtc tatgtgaagc cattcaagaa atcgaaggta cacctattta
    33901 taatgaactc aaagaaattc aaggcatgtg gtttagtagc ttggaagaat tactggaaaa
    33961 caaagaggat attcattgtt atagtgattg tgattcgatg gaagatgtcg cccgttatta
    34021 cgtggaagaa acaggacagc ttggtgaagt cccttccaat ttacaaaatt atattgacta
    34081 ccaatctttg ggacgtgata tggaaataga gggtaattac ctagttacct ctcatggggt
    34141 atttgaatat tgtcagtaag tttaacaatc cgtgttataa ttaaattaat aaatagcacg
    34201 gagaggtata aattatggta aatgtatttg aaaatcttaa actattaaaa ctagacatga
    34261 aagataaagg ctgggtgata gattcctttt actttcgtta caaacagcaa aattatattg
    34321 tacttgttaa gctatttgaa aaagaggaaa aagttccaga atatgctctg ttaaaactag
    34381 aatttttaaa ggaaaacgat ttttcagata tgctagctgt ctatgctaat tcggtaaaat
    34441 tatatacaga cacgaaaaca ataagagaat attttggtat cgaatacagt agtaacttgg
    34501 gagatgtttt atttcaattt agccaaactc ttgctcgatt tattccgaca gaagttagtg
    34561 agaagaaaaa tgaagaccaa aaagaagcca tgtgtttctc attaagtcaa agtgattcag
    34621 aagatcccag aaaaaaatat tgtttttcag ttagaagaaa tcctttaaag ggaaatggtg
    34681 agctgggaaa acgaagtcct tttaatgaca ataaaacgag attgtataga cccagcttat
    34741 atgaacgatt agggtcagat accaatttaa gtttcagata ttcaatgaat ccagatgatg
    34801 aagaaactga tgaaggtatt attgcaaaat ggacaaaaaa taaaattgaa tagttaaagc
    34861 attgaaagag caatcaccta ttttgattgt tctttttcta tttttacgga aaggacgtga
    34921 agacatgaaa aaaataagaa gttacaccag tatttggtca gtagaaaaag tgatttatgc
    34981 gattaatgat ttacaactcc ctttcccaat tacctttacg caaatgacat ggtttatcgt
    35041 ttctttgttt gtcgtcatac tctttggcag tttaccacca ctttctctga ttgacggagc
    35101 actactcaaa tacttaggga ttcccattgt tctcactggg ttcatgtgcc aaaagacctt
    35161 tgacggaaag aaaccctatg gatttctaaa gtctatcatt acctatgctc tacgccctaa
    35221 agtaacctac gctggcaaac aaatacgatt aaaaaaagaa acaatgaatg aatcgattac
    35281 gattgtaagg agtgaacaac tagatggcat atccgattaa atatatggaa gataatctcg
    35341 tatttaatca tgatggcgaa gtattcgctt attatgaatt aattccctat aattacagtt
    35401 ttttaagttc cgatgaaaaa attcaagttc atgataattt ctgtcaattg attgcccaaa
    35461 atcgtgatgg caagattcat gctctgcaac tagcaaccga atcaagtatt cgagccacac
    35521 aggaacaatc gaaaaaagaa atcactggac gattaaaaga gatagcttac gaaaaaatag
    35581 atgaacagac cgaagcgtta atttctatga ttggtgacaa tcaagtagac tatcgctttt
    35641 ttattggttt taaactctac ctcaatgaac aggaagtatc cgtaaaaaat gttgggaaag
    35701 aaattcaaaa agcatttgcg gaattctttc atggtgtgaa tcacaaatta atgggcgatt
    35761 ttgtttctat gcctatggcg gaaatcgaac gcttttctaa gatggaacag ttgttagaaa
    35821 gtaaattatc cagacgcttt cagattcgta aattggagaa agatgatttt ggttatatta
    35881 ttgaacatct ttacgggcaa acaggtgtcc cttttgatga ctacgactac catttaccga
    35941 aacgatacga agataaaaaa gtgttggtaa agaaatacga tattctcaaa cccactcgct
    36001 gtttaatcga agaaaatcaa cgctatttac ggattgaaca ggaagatacc actacttatg
    36061 tggcgtattt tacaattaat agtattgttg gtgaattgga tttccctaat agtgaaattt
    36121 tttattacca gcaacagcag tttactttcc ccgttgatac cagtatgaat gttgaaattg
    36181 tcgccaataa aaaagcctta tctaccgttc gcaataaaaa gaaagaactg aaagatttag
    36241 atagccacgc atgggaaagc gacaacgaaa ccagtactaa tgtggtggac gctttagaat
    36301 ctgtcgatga attagaaagt aatttagacc aaaccaaaga atctatgtac aaactaagct
    36361 atgtggtacg ggtatcagca aagaatttgg acgagctgaa acaacgttgt gatgaagtaa
    36421 aagattttta tgatgatctg tctatcaaat tagtacgacc ttttggcgat atgctaggct
    36481 tgcataatga atttattccc tcaagcaagc gatatatcaa tgactatatt caatatgtca
    36541 cgtcggattt tttggctggt cttggtttcg gtgctacaca aatgattggt gaaaattctg
    36601 gtatttacgt cggttacaat ctcgatacag gcagaaatat ttatctccaa cctagccttg
    36661 caagtcaagg aattaaaggc tctgtaacaa atgcattagc gtccgctttt attggttcat
    36721 taggtggcgg aaaatcattc tctaacaata tgctggtcta ttattcagta ctctttggcg
    36781 gacaggcaac cattgttgac ccaaaagctg aacgtggtga atggaaagaa accttacctg
    36841 atattgccca tgaaatcaat attgttaatc tcacgagtga gaacgacaat aaagggttac
    36901 ttgaccctta tgtgatttta aaactcccga aagattcaga aagccttgcc attgatattc
    36961 tcacgttctt aacaggtatt tccagccgag atagcgagaa gtttcctgtc ctgcgtaaag
    37021 ctattcggag tgtaactcaa agcaaacaac gagggttgtt attggtgatt gatgaattgc
    37081 gaaaagaaga tacgcccatc agcaatagta ttgctgacca tatcgaaagc tttgtcgatt
    37141 atgattttgc ccatctgtta ttcagtgatg gcagtgtcac ccaatccatt agcttggaaa
    37201 aacaactcaa tattattcaa gtggcagatt tggtcttacc cgattccaat agctcttttg
    37261 aagaatacac taccatggaa ttattaagtg tggcaatgct gattgtcatt agtacctttg
    37321 ctttagactt tatccactct gaccgttcca tttttaaaat tgttgacctt gatgaggctt
    37381 ggagtttttt acaagtggca caaggaaaag ctctatctat gcgattggtt cgagcaggac
    37441 gtgccatgaa tgcaggggtc tatttcgtta ctcaaaacgc ggacgattta ctagatgaaa
    37501 agatgaagaa taatattggc ttgaaatttg cctttcgttc cactgaccta gtggaaatca
    37561 aaaagacctt agaatttttt ggtgtggaca aggaagatga aaacaatcag aaacgcttac
    37621 gtgaattaga aaatggacaa tgtttagttt cagacatcta tggtcgtgta ggggttatgc
    37681 agtttcaccc gatttttgaa gagttgcttc atgcctttga taccagaccg cctgtgcgaa
    37741 atgaggtgga agaatgacac aaaaacaaaa gaaatttctt cactatagtt ggataacgct
    37801 cctttgtgtg ggcgttattt tacttttatt aggcacgctg ggcaatgcag tacaagcgac
    37861 aggcttagtt gatgaaacca ttgatacaag caatgaatac tccaagtatg gtttgaatca
    37921 atatcagctt gattattatg tggacaatag ctggggctgg cttccatgga attggagtga
    37981 tggcatagga caatcggtga tgtatggatt gtatgccatt actaatttta tttggacaat
    38041 ttctctttat ctatcgaacg ccacaggtta tttggtgaaa gaagcctatg ccttagattt
    38101 cattagtcaa acatcccaag caattggcga aaacatgcag acgattgcag gcattacacc
    38161 gaatggcttt tccagttcag ggttttatgt cggtttcctc cttcttttta ttctcattgt
    38221 tggaatctat gttgcctata caggattggt taagcgagaa acgacgaaag tgatacgagc
    38281 tgttttaaac tttgtcgtgg tgtttattct atcggcttcc tttattgcgt atgcacccga
    38341 ttatattgcg aaaatcaatg atttttccaa agatgtcagt accgccagtc tgaatattgg
    38401 gacaaaaatt gttcttccta acacggatag tcaagggaaa gacagtgtgg acatgatacg
    38461 tgacagctta ttctctgttc aagtaaagca accatggcta ttgcttcaat atggctccac
    38521 tgatgtagaa gcgttagggg aagaacgtgt ggaaagttta ttgtctacca gtccagatac
    38581 aaacaatggt gaagatagag aaaatgtagt gattgaagaa attgaagatc atgacaatac
    38641 ctatttaacc ctaccgaaga cgatttccag attaggaaca gtcttctttt tgtttgtatt
    38701 caatattggt atctcgattt ttgttttcct tctaacagga atcatgattt tttcgcaagt
    38761 gctatttatt attttcgcta tgtttttgcc aatcagtttc ctattaagta tgttgccgag
    38821 ttttgatggc atgggcaggc atgccattac caagttattt aatgtgatta tgacaagggc
    38881 tggtattacc ttgattatta ccgttgcctt tagtatttcc actatgcttt attcgctgtc
    38941 agcaagttct ccctttttca tgattatgtt tttacaaatt gtgacctttg cagggattta
    39001 ttttaaactg ggcgatttaa tgagcctatt cactttacaa agcaacgact ctcaaaacat
    39061 gggcagacaa gtgtttcgca gacctcgtca aatgatgaat cgacaaacca gaagatttca
    39121 acgcagtatg agtaagattt ttcgtggaaa agcagggtca acgaagcaaa agacaatgac
    39181 aacaaagcag aatcaaacca ataaagctaa ccatactcgt caaaatgaac ggactacacc
    39241 aaagaaagaa acagcacttt ccaatgtgaa aaagcaacaa ggcggtatcg ggcaacgatt
    39301 aggtgaaaag acagcccatg tattggacac gaaaaatcgt ctagtcaata aaagaaagca
    39361 ggcaaaacaa cagatacagg cgacaccaac ccatgtcaaa tatgcccttc atcaaggaaa
    39421 agagaatgtc actcataatg taacagactt taaacaatct atggcagata ccaaagctca
    39481 acgcaaacaa cagcgtatga accagcagga gcaaaaacga aagaacattg ctcaaaagcg
    39541 tttggagctg gataaagcaa aagaaaaaag acgaaaagaa aacccatcat ctcctgttca
    39601 gaataaccag cagcatgttc gcaagcgtca gcaaaccgtc acgcctgttt tgtcacaaaa
    39661 cactaaacgt gaaaatcatc agcgaccatt gaaaaaagag caaaaacaaa agacaccacc
    39721 gaagcaattc aatcagagga agctaaataa aagaacggat aagcgaggac aacaaacatg
    39781 aagctaaaaa taacagtgat ttgtagtgtg gtcttctctc tcttcttttt tctcttatta
    39841 ttcctagtca ttttctttgc agatgatacg gacagtggcg aaaacaacaa ggattcttct
    39901 attccacaag gcggtgtgac cgtttcacca gaagtgttgg cacaccgtcc gttgattgaa
    39961 aaatatggta aggaatatgg gattgaagac tatgtttctt atatcttagc cattatgcaa
    40021 gtggaatcgg gcggtacagc agaagatgtt atgcaaagtt ccgaatcttt gggtttaccg
    40081 cccaacagtt tgagtaccga agagtccatc aaacaagggg ttaaatattt cagtgaatta
    40141 ttaaccagtg cagaacaaca aggagtggat atagattctg ttatccaatc ttataattat
    40201 ggcggtggct ttttaaatta tgtgaggagt catgggaaaa aatataccta tgaattagcc
    40261 gaacagtttt ctaaagagaa gtcgggtggt caaaaagcag actatcccaa tcctatagcc
    40321 atacctgtga atggtggttg gcgatataac tatggaaatc agttttatgt tcagttggtt
    40381 tcacaatacc taaccgacac ttccccaaca gaatttgatg gtgaaaccgt gcaagtcatt
    40441 atggacgaag ccttgaaata cgaagggttt ccctatgtct ttggaggggc gtcgcctacc
    40501 acctcttttg attgtagtgg cttaatacaa tgggtgtatg acaaagctgg catttctctc
    40561 cctcgtgtcg cccaagatca gtatgacgct acgcaagaaa tctctatgga agaagcccaa
    40621 gcaggcgacc ttatcttctt tcattcaacc tataatgcag gcacatatgt gactcatgtg
    40681 gcgatatatt tagagggaaa tcgcttttat catgcaggcg acccaatcgg ttatggcgac
    40741 ttaagcagtc gttattggca agaccatctg attggggcta gacgtgttat tcataactaa
    40801 gaaaggaatg aatcaaatga ttataaaaat cgagcgaaaa gaaaagaagg agaaaccaaa
    40861 gaaatctaaa aaaccgagca agcaaagaaa agttccgatg attaaagttg gcacgcataa
    40921 gaaattaacc tttgttttat gggttttatt gattggtagt gtcggatttg gaatctataa
    40981 aaattttact gccattgaca ctcatactgt ccgtgaaaca gaaatcatta aacagcagat
    41041 tgtcgatacc aaccaagtag aaagttttgt gaaatccttt gcgaaagact acttttcgtg
    41101 gcaacaatcc caagaagcca ttgataaacg aaatgaaaaa ttgacccatt atttgacgga
    41161 agaattgcag gtattaaatg aagaaatgat tcgtaaggat attcctacta gttctagtgt
    41221 gaacgatata caggtgtggc aggtttctca agtgaatgag aacacctttg aggtactgtt
    41281 ttctgttgaa caagttatta ccgaagacaa agacaaggaa actatatcgt ctagctttca
    41341 tgtggttgtc catatagatg aatcagataa tatggttatt atcaaaaatc cgacaatgag
    41401 taagaaacct caaaaatcgg actaccaacc caaacagcta gaaagtgacc atacagtaga
    41461 tacagaaacg atggacgaaa tcactagctt tttagaaacg ttcttccagc tctatcctac
    41521 cgccacagaa aaagaattga cttactatgt aagtaatcat gtgttaccta tgattaacaa
    41581 ggaatatgtt ttcgaggaat tggtgaatcc tatctttacc agaaaagata accaagtaat
    41641 cgtgaatgtc gctgtgaaat atttagatca agaaacaaag gcaacccaaa tctcgcaatt
    41701 tgagttgata ttagaaaaac aagataattg gaagattgta aaataacaaa tattggtaca
    41761 tgattacaga tactttgtaa tcatgtactc tttttgataa aaaattggag attcctttac
    41821 aaatatgctc ttacgtgcta ttatttaagt gactatttaa aaggagttaa taaatatgcg
    41881 gcaaggtatt cttaaataaa ctgtcaattt gatagcggga acaaataatt agatgtcctt
    41941 ttttaggagg gcttagtttt ttgtacccag tttaagaata cctttatcat gtgattctaa
    42001 agtatccaga gaatatctgt atgctttgta tacctatggt tatgcataaa aatcccagtg
    42061 ataaaagtat ttatcactgg gatttttatg cccttttggg tttttgaatg gaggaaaatc
    42121 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcaggaaaa actaccttaa
    42181 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
    42241 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
    42301 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
    42361 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
    42421 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
    42481 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
    42541 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
    42601 tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
    42661 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
    42721 tcgaacaaga ggaaagcata agatttcaga attgttctct gttccctctt tatcatggaa
    42781 gtgcaaaaag taatataggg attgataacc ttatagaagt tattactaat aaattttatt
    42841 catcaacaca tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa
    42901 aaaaaagaca acgtcttgca tatatacgcc tttatagtgg agtactacat ttacgagatt
    42961 cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact tcaataaatg
    43021 gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg caaaatgagt
    43081 ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg
    43141 aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa
    43201 tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg
    43261 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
    43321 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
    43381 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
    43441 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
    43501 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
    43561 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
    43621 gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt cggatgctta
    43681 ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta gagccatatc
    43741 ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac gatgctccca
    43801 aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt attattggag
    43861 aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt acaaatgggc
    43921 ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa cctgtttgcc
    43981 agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat aaaataactt
    44041 agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga aatattcttt
    44101 aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta caagtggata
    44161 ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac tatacttaaa
    44221 aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt tcaaaaaatg
    44281 aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt tgagaaatga
    44341 aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc cttcaatatt
    44401 tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt tgtctgtaaa
    44461 gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg
    44521 taagaaaaat tcatcaatag atacatggag taatgataca agatcataca gcacttgtgt
    44581 acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa tttcaatcat
    44641 tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa tcgctaaacc
    44701 aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc tctatacatt
    44761 ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag tttatgagtt
    44821 cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata gaaaggtcgc
    44881 tgattttatt tacctataca actttataaa atttagtcat actcctagaa agtagataaa
    44941 attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca tctagttctc
    45001 ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat gaccatcaat
    45061 gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga aaacaatctg
    45121 tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc atttatgcaa
    45181 caaatccccc tttcgtgtcg gggtttagta tgaagggaga taaagaagca catgccactc
    45241 cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag
    45301 aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa gattacttca
    45361 aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat aactaccttg
    45421 tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt aaattatttg
    45481 gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat ctttcagaga
    45541 aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg gagattgcgg
    45601 agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga ctagccatga
    45661 ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg ttcccttccc
    45721 cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg ttaaacatta
    45781 tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg gaaattctca
    45841 tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct ctaagatttt
    45901 atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg tatccacgct
    45961 tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt aggttttgat
    46021 aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata cgtttcggta
    46081 agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg attcatgtta
    46141 gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg tgataatgat
    46201 actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt acggctacat
    46261 gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga ttgctaagat
    46321 attcttctct tgtcagtgta ttattataaa actatttgat ataattatag gtgaatattg
    46381 gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac aaaaaaggat
    46441 ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa agcaaaagaa
    46501 ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag agtccctcgt
    46561 gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc tgagtagttt
    46621 ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca aagaatggaa
    46681 cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt caaaaatatc
    46741 gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg ctattgatga
    46801 aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat atctttgaaa
    46861 ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat cgcttaccaa
    46921 ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt gagcctattg
    46981 atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat gtaagagctg
    47041 ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata accaccacaa
    47101 atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa ttttggacaa
    47161 aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat caacactttt
    47221 tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa gctactgcca
    47281 ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa acactctatt
    47341 acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc gttcgtcata
    47401 ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta caacaaaatc
    47461 taattcagac agactttgta atgagctata acggtattcc tacccaaaaa catacactat
    47521 catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga atccatgggt
    47581 tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt atcattaaag
    47641 atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat ctttatccta
    47701 acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa gaagcaaaac
    47761 aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga aacaaagaaa
    47821 tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac ccttggtata
    47881 agaggatttc tagctctcct acaactattc ccactccact gttgctggtg gtttagaagt
    47941 gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt
    48001 ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac
    48061 agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg
    48121 gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat
    48181 ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc
    48241 gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc
    48301 aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg
    48361 ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat
    48421 cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag
    48481 gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa
    48541 ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa
    48601 gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc ctagactttc
    48661 cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa
    48721 ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga
    48781 aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc
    48841 gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg
    48901 cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa
    48961 attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc
    49021 aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt
    49081 tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata
    49141 ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc
    49201 atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc
    49261 attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc
    49321 ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg
    49381 aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc
    49441 aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat
    49501 ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga
    49561 tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt
    49621 cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt
    49681 cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca
    49741 gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat
    49801 agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg
    49861 tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa
    49921 tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta
    49981 cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt
    50041 ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca
    50101 gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg
    50161 tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt
    50221 atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat
    50281 aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat
    50341 tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa
    50401 attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct
    50461 cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt
    50521 aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc
    50581 ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat
    50641 ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca
    50701 atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc
    50761 acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt
    50821 acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt
    50881 ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct
    50941 agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat
    51001 ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc
    51061 tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg
    51121 tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga
    51181 agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag
    51241 tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca
    51301 tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt
    51361 tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga
    51421 tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac
    51481 atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta
    51541 gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg
    51601 tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga
    51661 caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt
    51721 caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt
    51781 tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga
    51841 tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta caagagcttt
    51901 cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg
    51961 cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc
    52021 gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc
    52081 tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc
    52141 aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta
    52201 atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa
    52261 aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg
    52321 gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag
    52381 gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc
    52441 caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc
    52501 aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag
    52561 tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt
    52621 agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata
    52681 atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga
    52741 aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta
    52801 tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca
    52861 agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt
    52921 catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg
    52981 tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa
    53041 aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc
    53101 cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt
    53161 agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg
    53221 tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa
    53281 aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat
    53341 gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc
    53401 taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg
    53461 aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct
    53521 agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac
    53581 aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa
    53641 attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg
    53701 aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa
    53761 aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa
    53821 agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt
    53881 gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat
    53941 tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca
    54001 tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa
    54061 agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga
    54121 agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa
    54181 tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac
    54241 agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga
    54301 tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc
    54361 aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg
    54421 tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc
    54481 ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac
    54541 aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg
    54601 tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg
    54661 tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg
    54721 ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca
    54781 gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt
    54841 ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga
    54901 ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca
    54961 tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt
    55021 cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat
    55081 ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat
    55141 ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag
    55201 gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag
    55261 ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct
    55321 catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat
    55381 tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag
    55441 gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg
    55501 accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag
    55561 cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca
    55621 tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg
    55681 caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa
    55741 aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga
    55801 aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag
    55861 ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg
    55921 cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg
    55981 acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat
    56041 taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag
    56101 tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt
    56161 atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa
    56221 aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc
    56281 ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg
    56341 tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg
    56401 aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta
    56461 gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat
    56521 attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac
    56581 atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc
    56641 aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg
    56701 atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg
    56761 atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg
    56821 ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa
    56881 aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca
    56941 gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg
    57001 atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc
    57061 atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa
    57121 atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc
    57181 aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca
    57241 ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta
    57301 gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct
    57361 ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct
    57421 ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact
    57481 aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat
    57541 aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat
    57601 caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga
    57661 gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta
    57721 tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg
    57781 ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa
    57841 cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa
    57901 gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc
    57961 gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta
    58021 gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa
    58081 attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat
    58141 attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc
    58201 ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac
    58261 agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca
    58321 agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa
    58381 agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc
    58441 tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat
    58501 gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta
    58561 tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg
    58621 gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc
    58681 gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt
    58741 tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt
    58801 gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac
    58861 gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt
    58921 ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat
    58981 tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa
    59041 tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat
    59101 ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc
    59161 aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg
    59221 atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact
    59281 ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct
    59341 tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg
    59401 atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca
    59461 acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct
    59521 gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga taacatcacg
    59581 cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat tggaccaatt
    59641 ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt cctagcttgg
    59701 gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga aaatcctcaa
    59761 gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt actgatttct
    59821 ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg taacttttca
    59881 gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt tggtaagtgg
    59941 aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag cttgagtatc
    60001 attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca aattgctcca
    60061 tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg tccaaaagca
    60121 aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg gtgggttacg
    60181 ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag
    60241 gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga attggagaag
    60301 caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac attgcgaaag
    60361 aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc cgtcctcttg
    60421 aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa gaagtttctg
    60481 tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc aaaactccat
    60541 ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa gaattttctg
    60601 gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac gattacggtg
    60661 aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct gtccttcaga
    60721 tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc caattcgttc
    60781 gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg
    60841 gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg aatccattcg
    60901 gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct gttggaaaga
    60961 tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg
    61021 atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc agctttacca
    61081 atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg
    61141 cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa gaagacgact
    61201 tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc gatcatacgc
    61261 gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg gcattgcctc
    61321 aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg
    61381 aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga gaaagcaaat
    61441 gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata aaggagtaaa
    61501 agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag atgaaatcga
    61561 agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga cagtagttgg
    61621 gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag ccatgcaagg
    61681 gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag tacgtgtgat
    61741 ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg tcaacgaatc
    61801 atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg gtgaaaatcc
    61861 tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga gtcaagctta
    61921 tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt tggatcttaa
    61981 agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg agatccgaat
    62041 gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag tcatcgtagc
    62101 agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag cagcaggtat
    62161 gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca aacaatcttt
    62221 taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat aaaatgagtg
    62281 tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg ggagatcctt
    62341 tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac aatcaggtac
    62401 gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt caaggaacag
    62461 gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg tatggtgtaa
    62521 agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat gttcgtgatt
    62581 tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct
    62641 ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag atagctaaag
    62701 aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc tacaaagatg
    62761 atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa atggaagcag
    62821 cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg atgaccgtta
    62881 gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct acgttcaatg
    62941 aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat gaaaaagctg
    63001 ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt ttgacttggc
    63061 tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt tgtgaa
//
LOCUS       DACBZN010000006        56980 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.006, whole genome shotgun sequence.
ACCESSION   DACBZN010000006 DACBZN010000000
VERSION     DACBZN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.006"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            347..3181
                     /locus_tag="IVD64_000367"
     CDS             347..3181
                     /locus_tag="IVD64_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6147644.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3372..3863
                     /locus_tag="IVD64_000368"
     CDS             3372..3863
                     /locus_tag="IVD64_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP6147645.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3966..4955
                     /gene="manX"
                     /locus_tag="IVD64_000369"
     CDS             3966..4955
                     /gene="manX"
                     /locus_tag="IVD64_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP6147646.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4988..5791
                     /locus_tag="IVD64_000370"
     CDS             4988..5791
                     /locus_tag="IVD64_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP6147647.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5812..6726
                     /locus_tag="IVD64_000371"
     CDS             5812..6726
                     /locus_tag="IVD64_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP6147648.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6809..7183
                     /locus_tag="IVD64_000372"
     CDS             6809..7183
                     /locus_tag="IVD64_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP6147649.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7322..7567
                     /locus_tag="IVD64_000373"
     CDS             7322..7567
                     /locus_tag="IVD64_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6147650.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7739..8803
                     /locus_tag="IVD64_000374"
     CDS             7739..8803
                     /locus_tag="IVD64_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6147651.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9049..9474
                     /locus_tag="IVD64_000375"
     CDS             9049..9474
                     /locus_tag="IVD64_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6147652.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9467..9916
                     /gene="nusB"
                     /locus_tag="IVD64_000376"
     CDS             9467..9916
                     /gene="nusB"
                     /locus_tag="IVD64_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP6147653.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10013..10867
                     /locus_tag="IVD64_000377"
     CDS             10013..10867
                     /locus_tag="IVD64_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP6147654.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10868..12205
                     /locus_tag="IVD64_000378"
     CDS             10868..12205
                     /locus_tag="IVD64_000378"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP6147655.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12210..12434
                     /locus_tag="IVD64_000379"
     CDS             12210..12434
                     /locus_tag="IVD64_000379"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP6147656.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12436..13326
                     /locus_tag="IVD64_000380"
     CDS             12436..13326
                     /locus_tag="IVD64_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6147657.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13335..14153
                     /locus_tag="IVD64_000381"
     CDS             13335..14153
                     /locus_tag="IVD64_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP6147658.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14260..14709
                     /locus_tag="IVD64_000382"
     CDS             14260..14709
                     /locus_tag="IVD64_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6147659.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14727..16409
                     /gene="recN"
                     /locus_tag="IVD64_000383"
     CDS             14727..16409
                     /gene="recN"
                     /locus_tag="IVD64_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP6147660.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16464..16580)
                     /locus_tag="IVD64_000384"
     CDS             complement(16464..16580)
                     /locus_tag="IVD64_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP6147661.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16653..17609)
                     /locus_tag="IVD64_000385"
     CDS             complement(16653..17609)
                     /locus_tag="IVD64_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6147662.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17780..18148
                     /locus_tag="IVD64_000386"
     CDS             17780..18148
                     /locus_tag="IVD64_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP6147663.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18265..18750
                     /gene="mraZ"
                     /locus_tag="IVD64_000387"
     CDS             18265..18750
                     /gene="mraZ"
                     /locus_tag="IVD64_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP6147664.1"
                     /translation="MGDCGRLILVSGGMGGLSMLMGEFQHNIDAKGRLIVPSKLREEL
                     GEKFVLTRGLDGCLFGYPMSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQ
                     GRINIPSTLRNYAALTKGCVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFG
                     L"
     gene            18771..19730
                     /gene="rsmH"
                     /locus_tag="IVD64_000388"
     CDS             18771..19730
                     /gene="rsmH"
                     /locus_tag="IVD64_000388"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP6147665.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19735..20136
                     /gene="ftsL"
                     /locus_tag="IVD64_000389"
     CDS             19735..20136
                     /gene="ftsL"
                     /locus_tag="IVD64_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP6147666.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20137..22329
                     /locus_tag="IVD64_000390"
     CDS             20137..22329
                     /locus_tag="IVD64_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6147667.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22355..23317
                     /locus_tag="IVD64_000391"
     CDS             22355..23317
                     /locus_tag="IVD64_000391"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP6147668.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23331..24692
                     /locus_tag="IVD64_000392"
     CDS             23331..24692
                     /locus_tag="IVD64_000392"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP6147669.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24718..25806
                     /gene="murG"
                     /locus_tag="IVD64_000393"
     CDS             24718..25806
                     /gene="murG"
                     /locus_tag="IVD64_000393"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6147670.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25821..27041
                     /locus_tag="IVD64_000394"
     CDS             25821..27041
                     /locus_tag="IVD64_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP6147671.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27197..28522
                     /gene="ftsA"
                     /locus_tag="IVD64_000395"
     CDS             27197..28522
                     /gene="ftsA"
                     /locus_tag="IVD64_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP6147672.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28545..29786
                     /gene="ftsZ"
                     /locus_tag="IVD64_000396"
     CDS             28545..29786
                     /gene="ftsZ"
                     /locus_tag="IVD64_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP6147673.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29803..30480
                     /locus_tag="IVD64_000397"
     CDS             29803..30480
                     /locus_tag="IVD64_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP6147674.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30494..31093
                     /locus_tag="IVD64_000398"
     CDS             30494..31093
                     /locus_tag="IVD64_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP6147675.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31108..31395
                     /locus_tag="IVD64_000399"
     CDS             31108..31395
                     /locus_tag="IVD64_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP6147676.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31444..32226
                     /locus_tag="IVD64_000400"
     CDS             31444..32226
                     /locus_tag="IVD64_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP6147677.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32247..32954
                     /locus_tag="IVD64_000401"
     CDS             32247..32954
                     /locus_tag="IVD64_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP6147678.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33216..36014
                     /gene="ileS"
                     /locus_tag="IVD64_000402"
     CDS             33216..36014
                     /gene="ileS"
                     /locus_tag="IVD64_000402"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP6147679.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36063..37589)
                     /locus_tag="IVD64_000403"
     CDS             complement(36063..37589)
                     /locus_tag="IVD64_000403"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP6147680.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37604..38251)
                     /locus_tag="IVD64_000404"
     CDS             complement(37604..38251)
                     /locus_tag="IVD64_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP6147681.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38435..38764)
                     /locus_tag="IVD64_000405"
     CDS             complement(38435..38764)
                     /locus_tag="IVD64_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP6147682.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38941..39669
                     /gene="sfsA"
                     /locus_tag="IVD64_000406"
     CDS             38941..39669
                     /gene="sfsA"
                     /locus_tag="IVD64_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP6147683.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39685..40698
                     /locus_tag="IVD64_000407"
     CDS             39685..40698
                     /locus_tag="IVD64_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6147684.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40698..41975
                     /locus_tag="IVD64_000408"
     CDS             40698..41975
                     /locus_tag="IVD64_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6147685.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42038..44740
                     /locus_tag="IVD64_000409"
     CDS             42038..44740
                     /locus_tag="IVD64_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6147686.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44892..45209
                     /locus_tag="IVD64_000410"
     CDS             44892..45209
                     /locus_tag="IVD64_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6147687.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45239..45559
                     /locus_tag="IVD64_000411"
     CDS             45239..45559
                     /locus_tag="IVD64_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6147688.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45667..47127
                     /locus_tag="IVD64_000412"
     CDS             45667..47127
                     /locus_tag="IVD64_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6147689.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47195..47416
                     /locus_tag="IVD64_000413"
     CDS             47195..47416
                     /locus_tag="IVD64_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147690.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47447..47629
                     /locus_tag="IVD64_000414"
     CDS             47447..47629
                     /locus_tag="IVD64_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP6147691.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47629..48042
                     /locus_tag="IVD64_000415"
     CDS             47629..48042
                     /locus_tag="IVD64_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP6147692.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48165..49346
                     /locus_tag="IVD64_000416"
     CDS             48165..49346
                     /locus_tag="IVD64_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6147693.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49735..49808
                     /locus_tag="IVD64_000417"
     tRNA            49735..49808
                     /locus_tag="IVD64_000417"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49769..49771,aa:Arg,seq:cct)
     gene            complement(49877..51016)
                     /locus_tag="IVD64_000418"
     CDS             complement(49877..51016)
                     /locus_tag="IVD64_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6147694.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51315..51950)
                     /locus_tag="IVD64_000419"
     CDS             complement(51315..51950)
                     /locus_tag="IVD64_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147695.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52063..52698)
                     /locus_tag="IVD64_000420"
     CDS             complement(52063..52698)
                     /locus_tag="IVD64_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP6147696.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52732..53193)
                     /locus_tag="IVD64_000421"
     CDS             complement(52732..53193)
                     /locus_tag="IVD64_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP6147697.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53323..53754)
                     /locus_tag="IVD64_000422"
     CDS             complement(53323..53754)
                     /locus_tag="IVD64_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6147698.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53772..54092)
                     /locus_tag="IVD64_000423"
     CDS             complement(53772..54092)
                     /locus_tag="IVD64_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6147699.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54391..55167
                     /locus_tag="IVD64_000424"
     CDS             54391..55167
                     /locus_tag="IVD64_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP6147700.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55182..55385
                     /locus_tag="IVD64_000425"
     CDS             55182..55385
                     /locus_tag="IVD64_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6147701.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55401..55739
                     /locus_tag="IVD64_000426"
     CDS             55401..55739
                     /locus_tag="IVD64_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP6147702.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            55726..55905
                     /locus_tag="IVD64_000427"
     CDS             55726..55905
                     /locus_tag="IVD64_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147703.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(55948..56418)
                     /locus_tag="IVD64_000428"
     CDS             complement(55948..56418)
                     /locus_tag="IVD64_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147704.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            56505..>56980
                     /locus_tag="IVD64_000429"
     CDS             56505..>56980
                     /locus_tag="IVD64_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP6147705.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGG"
BASE COUNT        18930 a         9555 c        11927 g        16568 t
ORIGIN      
        1 aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt
       61 tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata
      121 ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta
      181 taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt
      241 tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat
      301 gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat
      361 tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga
      421 tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag
      481 taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt
      541 acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag
      601 ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat
      661 ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc
      721 tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg
      781 aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga
      841 ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat
      901 gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg
      961 gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc
     1021 tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga
     1081 aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg
     1141 ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt
     1201 tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc
     1261 aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag
     1321 tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg
     1381 atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg
     1441 gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa
     1501 aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga
     1561 ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa
     1621 gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca
     1681 tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga
     1741 atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc
     1801 tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt
     1861 tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc
     1921 ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta
     1981 ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt
     2041 acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac
     2101 agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt
     2161 agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca
     2221 agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt
     2281 taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac
     2341 ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt
     2401 gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca
     2461 ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg
     2521 tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga
     2581 attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa
     2641 agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat
     2701 tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga
     2761 atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg
     2821 tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat
     2881 gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt
     2941 caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc
     3001 attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt
     3061 ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat
     3121 gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg
     3181 aatacacaaa aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct
     3241 ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg
     3301 ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag
     3361 gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag
     3421 tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag
     3481 tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag
     3541 caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag
     3601 caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg
     3661 tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta
     3721 ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag
     3781 aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac
     3841 tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt
     3901 gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta
     3961 taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat
     4021 ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg
     4081 aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag
     4141 acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat
     4201 tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga
     4261 ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca
     4321 ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac
     4381 cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg
     4441 gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag
     4501 ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag
     4561 tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag
     4621 caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata
     4681 ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg
     4741 tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa
     4801 gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag
     4861 gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt
     4921 tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag
     4981 gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat
     5041 ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg
     5101 tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc
     5161 tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc
     5221 atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc
     5281 gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc
     5341 tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga
     5401 aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc
     5461 gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt
     5521 aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat
     5581 caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc
     5641 aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta
     5701 cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga
     5761 ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa
     5821 gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt
     5881 caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca
     5941 gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg
     6001 gaattcttta atacacaccc atacattgct tctccaattt taggggttac attggctctg
     6061 gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta
     6121 ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca
     6181 atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc
     6241 ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc
     6301 tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca
     6361 aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca
     6421 atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg
     6481 gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc
     6541 ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct
     6601 ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct
     6661 ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt
     6721 ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg
     6781 ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt
     6841 ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga
     6901 taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga
     6961 agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc
     7021 cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct
     7081 tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga
     7141 tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc
     7201 taagaggagg ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca
     7261 attatttgtc ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat
     7321 tatgagttgg ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat
     7381 caccagtaaa ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc
     7441 aagtattgga caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat
     7501 tccatctatt ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg
     7561 atcataagtt tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt
     7621 ttggctttat ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg
     7681 tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat
     7741 gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt
     7801 ggttaccagt ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc
     7861 tgttattact ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc
     7921 agcgcaggct caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc
     7981 aaacttagta cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt
     8041 tttagaatat tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat
     8101 gatcgaagaa ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg
     8161 tcatattgcg gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga
     8221 gattgaagtc gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc
     8281 ttttgatacg atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa
     8341 aaaaatcatc gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta
     8401 cgtttccgat atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat
     8461 ttatcaaata gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac
     8521 tggtgtccaa ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc
     8581 atttggacat tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc
     8641 ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta
     8701 cttaccagga atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa
     8761 tcgtgtgttg actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa
     8821 gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata
     8881 cactatagaa gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca
     8941 tagcagtttg cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat
     9001 agagaattcg gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa
     9061 aaaaatttga ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc
     9121 atcgaagtaa ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga
     9181 acttttgcca gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac
     9241 ttagcaaacg aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt
     9301 tcagttccta aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg
     9361 acggatatcg atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta
     9421 gaacaaccaa caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt
     9481 atcaagacac gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc
     9541 agacctaaca aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat
     9601 caacgaagat gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg
     9661 tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat
     9721 caatcggatc tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta
     9781 tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt
     9841 tagtgatgac cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt
     9901 agaagctggg gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc
     9961 gggaaaattc tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga
    10021 attgattaac ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca
    10081 aaaattgaaa gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc
    10141 agccagccaa atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc
    10201 attggtagag cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa
    10261 atacaatcaa gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat
    10321 agatgaagaa aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc
    10381 gatgaattta ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg
    10441 catcatgaaa atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat
    10501 cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac
    10561 tgttactgtt gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat
    10621 tttagtcgta gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc
    10681 tgtagtgata gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa
    10741 atttgatgaa gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc
    10801 aatgactatc acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg
    10861 tgaatagatg gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa
    10921 atttgatgcc gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg
    10981 tttacgtgcc aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc
    11041 tattatgttc aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt
    11101 ccttgtcgtt gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga
    11161 gcacatggag cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa
    11221 gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg
    11281 aatcgcggtc cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa
    11341 aagaagatat ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc
    11401 agcggacaat gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat
    11461 gatcatcggt cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt
    11521 tgctcgggcg atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga
    11581 taccacgatc gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga
    11641 attagctgtt cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt
    11701 gcagcaggcg tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg
    11761 ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca
    11821 gttgaaacag cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac
    11881 gatcgcatta agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg
    11941 ccaagaaact gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca
    12001 acagcagcag cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat
    12061 catgggaaga ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga
    12121 gattcatcaa ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt
    12181 acaagagata gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc
    12241 aagaattaga aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc
    12301 tagattcgtt taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag
    12361 cagaagagac cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa
    12421 acgaggaaag ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa
    12481 aaagaaatct tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg
    12541 agttattcag tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt
    12601 gcttcctttg atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg
    12661 gtgcatacgt attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt
    12721 agaggaaaac ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat
    12781 ggtttgctta caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag
    12841 cttctgctgc ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa
    12901 gctgcagata tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat
    12961 gaaagaaaga cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag
    13021 caacctgaag aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt
    13081 cagataagag atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct
    13141 ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca
    13201 cgtgaggcgt tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag
    13261 gacgtttttt cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag
    13321 aaatgaggga aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat
    13381 ttgaaacaag agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa
    13441 acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag
    13501 gtaaaaaact tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat
    13561 ttgatctatc tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta
    13621 ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc
    13681 agctagcttg gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc
    13741 gttatgcaaa acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt
    13801 cttttatttc actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag
    13861 aagtgattgc tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg
    13921 gaattgttcg tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg
    13981 cacaaggtgg atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa
    14041 atatcgagtt cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta
    14101 ttaatatggc agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa
    14161 ctgtgaaaat caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta
    14221 tcattgatta agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat
    14281 cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa
    14341 atcctgaaaa gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa
    14401 ctgaaactga tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa
    14461 acaaacgaag atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt
    14521 gaccaaatgg aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca
    14581 aatttgattg ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat
    14641 gttttgatga ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat
    14701 tacctttaag ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga
    14761 ttattccgga attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg
    14821 gtgcgggtaa atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa
    14881 gcgattatat acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa
    14941 aacaagaagg attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc
    15001 tgattgttcg acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata
    15061 ttatcacatt agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa
    15121 acgaacatca ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag
    15181 atgtagcttt ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc
    15241 tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata
    15301 tgctccattt ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga
    15361 aattaaaaga agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg
    15421 caggttacgg agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg
    15481 tttcagaact gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata
    15541 tccaaagtgc ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt
    15601 tattggagtt agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac
    15661 agttgaaacg taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca
    15721 ccgaagagct agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac
    15781 tagagcaaaa agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa
    15841 aaatcgcaaa agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa
    15901 acactgagtt tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat
    15961 ttgaccaaat cgaattttac attacgacca atccaggaga gccactgaag cctttggtga
    16021 aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt
    16081 ctgagcaagg tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag
    16141 ttgcacaggc catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca
    16201 tcactcatct gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag
    16261 tgattgatgg acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag
    16321 aaattgcacg tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag
    16381 aactattgaa actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag
    16441 cagctgatag ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat
    16501 ccaatcgtta aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt
    16561 gtttgttttt tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac
    16621 aatctttttt ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa
    16681 taggtagtta cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc
    16741 ctttcagcaa taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata
    16801 gttaaaataa tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg
    16861 attgctgaaa aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc
    16921 attgcctgct ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct
    16981 tggaaaagtt tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct
    17041 ctgaaataaa cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg
    17101 gatacttgaa gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg
    17161 ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata
    17221 tcactttttg gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta
    17281 attgtgataa gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga
    17341 aaataaccgc taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt
    17401 gtcaactttg attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa
    17461 gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta
    17521 ggacgttcta cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc
    17581 tcattatcta atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt
    17641 ataacggata gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca
    17701 taaatttaaa taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat
    17761 aaaaaaggag tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta
    17821 cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg
    17881 ttttaaaata aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt
    17941 gtctagaaat acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat
    18001 gggaatttgt attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta
    18061 tttcaagatg acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat
    18121 cattgtgaac atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa
    18181 aaggcttttt gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg
    18241 atttcccaga aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga
    18301 ggaatggggg ggctttcaat gttgatgggt gaattccaac ataatattga tgcaaaagga
    18361 cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc
    18421 ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct ggaagctaaa
    18481 ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt cttctattcg
    18541 gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag cacgttaaga
    18601 aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg aattgaaatt
    18661 tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt tgacgaaatt
    18721 gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta atgactgaaa
    18781 catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac atcaaaccgg
    18841 atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat ttattaagcc
    18901 aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg
    18961 ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc aaagcgaact
    19021 tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc attttgtatg
    19081 atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt tatcatcaag
    19141 atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat cacgtaatta
    19201 atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag gagaaatttt
    19261 ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc caaacaactg
    19321 gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc
    19381 atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg
    19441 aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga atcagcgtaa
    19501 tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa tatagtacgg
    19561 tacaagatct accaccggga cttcctgtca tacctgacga attccaacca gaactgaaag
    19621 tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac aatcgatcac
    19681 gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag aatgatggca
    19741 gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga tcagccggaa
    19801 caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa
    19861 cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc gattgctatt
    19921 gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc tgaaaaagaa
    19981 atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa
    20041 gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga aaaaggattg
    20101 tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg
    20161 cggtttatgg aaaagaaaaa tctaaatccg ataaacaatc gcaaaaaagt aggtatcatt
    20221 ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc gtatatcgtt
    20281 attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt gtaccaagga
    20341 agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt ggcgctcgca
    20401 gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac ctctggggat
    20461 aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct gcataagaat
    20521 ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa aaaagagctg
    20581 tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc
    20641 gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg
    20701 atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc agacaaagat
    20761 gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat cttgagcggt
    20821 aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc aggtacagtg
    20881 gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga cagcagacta
    20941 caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc agaagaattg
    21001 acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca acgacctact
    21061 ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg taatttcttg
    21121 gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc agctgctatc
    21181 gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca agtagaagat
    21241 gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt
    21301 tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg acgatggtat
    21361 aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga tgatgaagta
    21421 aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta tggacaagcg
    21481 gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa taacggtaca
    21541 atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga agagcgaaca
    21601 actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa
    21661 tatatgagag atgttgtcga aagcgaaaac tacggtagtg cttatggtgt atatagtgta
    21721 cctggataca atgtatcggc aaaaaccgga acagcccaaa tcgcttctga tacaggggga
    21781 tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc ttctgaagat
    21841 ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg aatggcattg
    21901 gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat
    21961 tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa tcttgaagcg
    22021 gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt
    22081 aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg agaaaaactg
    22141 atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc aaaagcagat
    22201 atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga tggttattgt
    22261 gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag ttttacttta
    22321 gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag cattgattcc
    22381 cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg gttattttca
    22441 aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca atagcaaagc
    22501 cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga caggaatttg
    22561 ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt ttgtattagc
    22621 gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga aaagaaacat
    22681 gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca tcttttatct
    22741 tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag atctaccttt
    22801 aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa
    22861 tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt ttgcagctta
    22921 cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct taagtgtttt
    22981 aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt ttatgggaga
    23041 tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc taaaccaaga
    23101 atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg tgatgcttca
    23161 agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa ttcatcatca
    23221 ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt tgatcagcat
    23281 tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa
    23341 tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg
    23401 cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg
    23461 acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata acaggtagtc
    23521 atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca gggattccct
    23581 acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt acggaagttg
    23641 agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg aacggaaaaa
    23701 caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa ggaattgcac
    23761 gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca aaatcagaag
    23821 ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat ttccgccctc
    23881 atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt acaagaaaag
    23941 aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat tatctgatcc
    24001 ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc
    24061 ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac tcaatatact
    24121 ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag cataatgtag
    24181 aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat gaagctatac
    24241 gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta ggagaaatcc
    24301 aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact aaaatggctc
    24361 taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac agaggaaata
    24421 catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta tttggccaaa
    24481 caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata gtaacagccg
    24541 attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt gatgtagttt
    24601 tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact agaggaaaca
    24661 gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg
    24721 aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct tgcctttgtc
    24781 aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc aaaacgaggt
    24841 ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga gattcaagga
    24901 tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct taagagtatc
    24961 cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg tactggagga
    25021 tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac agttatccat
    25081 gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt ggatcggatc
    25141 gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt
    25201 aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa aacatttgat
    25261 ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc acttaaaatc
    25321 aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca agttttgtat
    25381 gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga aatacaaaat
    25441 attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag tgatctgctg
    25501 gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt accggcagtg
    25561 ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat gagtcttgtt
    25621 cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc actaagtcag
    25681 acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa
    25741 gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa
    25801 aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt
    25861 cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat ctggcagaac
    25921 aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca cccctttcag
    25981 attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag tccgagaaaa
    26041 aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat aacggttctt
    26101 ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt cgtcggttag
    26161 ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat gtttctcctt
    26221 atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc caagaaatca
    26281 tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt catcgatcta
    26341 cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat atacatttca
    26401 aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct ttgatcgcaa
    26461 aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa aaagtagcaa
    26521 atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa atcaaagaat
    26581 taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct gaaatcaagt
    26641 acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac ggcaatcaag
    26701 ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag
    26761 atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat ccttacggta
    26821 gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt gaagaaaatg
    26881 cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa acacctggat
    26941 cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt caaatagaag
    27001 ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt
    27061 aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt gaatatatct
    27121 attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga atagtaagta
    27181 ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata ttggaacgac
    27241 atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta ttggagtagg
    27301 aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata aaacggtcca
    27361 agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt
    27421 aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa tgattgcagt
    27481 taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt cagctgccct
    27541 tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag attttactgt
    27601 cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat tagaaatgta
    27661 cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat gcgtggaaaa
    27721 tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg aatcaattct
    27781 ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg gccaaacaac
    27841 aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag aaggtggcga
    27901 atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg cagaagcgtt
    27961 gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag aatttccagt
    28021 agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt cagaaattat
    28081 ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc aaatcgaagc
    28141 attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc ctggtgttgt
    28201 ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg
    28261 gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag caaacttgag
    28321 tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat
    28381 ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg aagaaacaat
    28441 ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa tcaagggttt
    28501 ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa ttctcaattg
    28561 ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa
    28621 atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca
    28681 cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta ggccccaaat
    28741 atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa
    28801 gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc actgcaggaa
    28861 tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg
    28921 gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc
    28981 gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta ttaattatct
    29041 caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa gctttccgtg
    29101 aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc acagcgccag
    29161 gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat
    29221 tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa
    29281 tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt cttaacatca
    29341 caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg
    29401 cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa atgggcgatg
    29461 aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta
    29521 gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta ttagatatgg
    29581 atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg gatatcagac
    29641 gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc gaaaaaaaag
    29701 aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc
    29761 caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc tgacaacttg
    29821 cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa
    29881 gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga attggttaga
    29941 gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa gaaacaagat
    30001 ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg cagaaaggta
    30061 aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag attagcagaa
    30121 gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt
    30181 gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga atctttagct
    30241 gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga agcttctgat
    30301 gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa
    30361 ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga ctttcaaatt
    30421 gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa agacgaataa
    30481 aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt tcggattagc
    30541 tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg ctgtacagcc
    30601 aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg cgaatactca
    30661 tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc agccggctga
    30721 aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga aagaaagaac
    30781 agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga
    30841 accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg gagaagcaac
    30901 cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg attttctaac
    30961 aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga tttttctttg
    31021 tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag atgcgaattt
    31081 gtttgatctg taagaggagg aactacgatt tatactattg cactattact tcatttactt
    31141 caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct ctcttggttt
    31201 ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga accgtatatc
    31261 agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat tttaggggca
    31321 atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg gataactgtc
    31381 catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata ggaggggaaa
    31441 attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt tatcgattcg
    31501 gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac cgaatttctt
    31561 gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga tttgcgtttc
    31621 atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt ccccgattat
    31681 tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta tcctagcaaa
    31741 tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg gatcaaacgc
    31801 tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat tgcaaatgag
    31861 gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt acgcttggaa
    31921 ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga aagaggaaca
    31981 gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc cagacagcgg
    32041 tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa tacaagacct
    32101 gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag agtccagatc
    32161 caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt
    32221 aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc caaaataaga
    32281 cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc ttagatttag
    32341 ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac
    32401 attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac caatcaatca
    32461 tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga
    32521 tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa aaacgtgcac
    32581 atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat gcaacagaaa
    32641 aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga gtattccatc
    32701 aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg gaatgggatg
    32761 aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc aaagaagttt
    32821 tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag
    32881 aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg cctggtgaaa
    32941 atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg atgagagtgg
    33001 aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct tttccgaaat
    33061 tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca ctagatagat
    33121 gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac atgctttttt
    33181 gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat
    33241 taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa
    33301 aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta
    33361 gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta ggacattcat
    33421 tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc tatcgtgcac
    33481 catatgttcc aggatgggat acacatggat tgccgattga acaagtattg gctaataaag
    33541 gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt gaatatgcac
    33601 tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca ggagattggg
    33661 aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc gtatttggta
    33721 aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg tctccgtcaa
    33781 gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct ccatctattt
    33841 ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca gcttttgtca
    33901 tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta aatccaaaat
    33961 ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag
    34021 aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa tttgctggac
    34081 aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca cttgtgattt
    34141 taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct ccaggacatg
    34201 gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca cctgtagact
    34261 caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat gacaaagcaa
    34321 atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta gatttcttta
    34381 ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg gcaactccac
    34441 aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta gaaaaagtag
    34501 agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac cgcggcgatt
    34561 gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat gctgaaaatg
    34621 gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt gctgaacatg
    34681 gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga tttacccacc
    34741 caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc tggtttgatt
    34801 ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc ccagcagata
    34861 tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc acgactagtg
    34921 tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt gctttagatg
    34981 gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa aaagtgatca
    35041 aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat gaagcagatg
    35101 ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa atcagaaata
    35161 cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat actgtttcgt
    35221 acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa gtaatcaaag
    35281 aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt acagttatga
    35341 acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat gtagtttata
    35401 ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat caaacagctg
    35461 tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa atctggtcat
    35521 tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat gaagagtttg
    35581 caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt gataatgtct
    35641 taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg gaagcaaaac
    35701 taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat gcagatattg
    35761 cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat gaacaagtgc
    35821 cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca gacggggaaa
    35881 cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc
    35941 tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta gctgaaggat
    36001 tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac ccccttttgt
    36061 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
    36121 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
    36181 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
    36241 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
    36301 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
    36361 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
    36421 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
    36481 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
    36541 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
    36601 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
    36661 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
    36721 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
    36781 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
    36841 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
    36901 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
    36961 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
    37021 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
    37081 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
    37141 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
    37201 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
    37261 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
    37321 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
    37381 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
    37441 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
    37501 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
    37561 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
    37621 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
    37681 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
    37741 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
    37801 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
    37861 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
    37921 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
    37981 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
    38041 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
    38101 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
    38161 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
    38221 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
    38281 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
    38341 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
    38401 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
    38461 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
    38521 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
    38581 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
    38641 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
    38701 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
    38761 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
    38821 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
    38881 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
    38941 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
    39001 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
    39061 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
    39121 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
    39181 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
    39241 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
    39301 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
    39361 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
    39421 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
    39481 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
    39541 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
    39601 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
    39661 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
    39721 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
    39781 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
    39841 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
    39901 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
    39961 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
    40021 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
    40081 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
    40141 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
    40201 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
    40261 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
    40321 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
    40381 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
    40441 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
    40501 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
    40561 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
    40621 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
    40681 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
    40741 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
    40801 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
    40861 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
    40921 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
    40981 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
    41041 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
    41101 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
    41161 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
    41221 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
    41281 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
    41341 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
    41401 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
    41461 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
    41521 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
    41581 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
    41641 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
    41701 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
    41761 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
    41821 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
    41881 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
    41941 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
    42001 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
    42061 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
    42121 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
    42181 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
    42241 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
    42301 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
    42361 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
    42421 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
    42481 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
    42541 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
    42601 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
    42661 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
    42721 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
    42781 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
    42841 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
    42901 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
    42961 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
    43021 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
    43081 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
    43141 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
    43201 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
    43261 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
    43321 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
    43381 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
    43441 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
    43501 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
    43561 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
    43621 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
    43681 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
    43741 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
    43801 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
    43861 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
    43921 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
    43981 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
    44041 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
    44101 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
    44161 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
    44221 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
    44281 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
    44341 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
    44401 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
    44461 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
    44521 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
    44581 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
    44641 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
    44701 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
    44761 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
    44821 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
    44881 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
    44941 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
    45001 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
    45061 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
    45121 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
    45181 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
    45241 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
    45301 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
    45361 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
    45421 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
    45481 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
    45541 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
    45601 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
    45661 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
    45721 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
    45781 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
    45841 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
    45901 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
    45961 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
    46021 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    46081 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    46141 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    46201 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    46261 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    46321 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    46381 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    46441 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    46501 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    46561 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    46621 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    46681 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    46741 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    46801 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    46861 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    46921 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    46981 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    47041 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    47101 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    47161 gttcctcata atgaattttc taggaggaac tgaaatgatt acaattgcca gtgcaatgca
    47221 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    47281 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    47341 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    47401 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    47461 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    47521 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    47581 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    47641 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    47701 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    47761 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    47821 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    47881 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    47941 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    48001 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    48061 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    48121 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    48181 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    48241 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    48301 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    48361 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    48421 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    48481 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    48541 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    48601 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    48661 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    48721 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    48781 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    48841 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    48901 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    48961 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    49021 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    49081 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    49141 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    49201 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    49261 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    49321 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    49381 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    49441 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    49501 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    49561 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    49621 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    49681 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    49741 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    49801 caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt tgtatttgtg
    49861 ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca gtattgattt
    49921 tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac attgtagttt
    49981 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
    50041 aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca atccctaatt
    50101 cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat ccatttttta
    50161 agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat aattcttttt
    50221 gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa attttcctat
    50281 ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat gttttattaa
    50341 tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga agttcagcaa
    50401 atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac tgcgcagaaa
    50461 ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa tatttatctt
    50521 cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga tcaaacggaa
    50581 ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta aacttaataa
    50641 cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat cccactctat
    50701 ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac gtatcttcgt
    50761 attttctata agtgatagga gatactgttc cttctttata aagttgcatc caatcctcaa
    50821 agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc aagttatatt
    50881 ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat tttcttatct
    50941 tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta tttccacgtt
    51001 tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg aactataaag
    51061 aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc ggcgcaagga
    51121 tgtgctattt gtcgttttaa tggtataatt ggatgtgtat tagatcatcc aatcagaggt
    51181 gtttattatg attaaagtga tttttaaaag aataactgaa cttccagagt ttagattatt
    51241 tgttgattat tggtatgtct ggttttttat tgttgcattg ctgataggtc tagatgtcat
    51301 attagaaaga aaacttaagt aagagttgag ttaataaatt gaattcctaa atgagatatt
    51361 ttgtagccca tattttgttt gccgacatca cctaatacta tggagctaat cgttttttct
    51421 aacagaccaa aatcagataa tctatttaag ctaccatcta ttagctcttg tgaatagcca
    51481 gacatgctga tatttccagt gaaattttcg tttccacgac tcattaagca tgctaacaat
    51541 tgaatctcaa tcgatgttaa ttgattcagt acaagaagat aatactcctc gttatcccat
    51601 gaagcgctgt taattcgaag taaacaattc ttataaagat ttttatacat atctatttta
    51661 ttttgtgcct ttgatttttc tacctcgtca tttatccctt cgaatattcc aatgagttgg
    51721 tccttgttct ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt ttctaatgat
    51781 tcgtttaaat ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt tttagaccca
    51841 aaataaagtg actgcacaac gccaccaata taaggaattg ctccaatacc gccttcaact
    51901 attaattcaa gatattcttt tttggttagt ttttcattgc tattatccat agttacctcc
    51961 tattttgtca tttcctttac aagcattgtg actcctgatg gcactctttt gtataggttt
    52021 tattattgtg cacaatctaa ctcttagtgg ggcgtggttc atttattttt tacttacttt
    52081 actattaatc atatcccatt gagcatagga tttttctatg ttcgatcctc ttgaactaat
    52141 ttctcctgta aaagttactt tatcgccctt tgtaaaggta tcagcattgt ttaagtcctt
    52201 cgcaaaaatt acatatgaaa ctttaggtgt tgcagaaata tcattccaac ttttatcatc
    52261 gtactctttg tctgctataa tggctattct agtagtcatt gattcgataa cagttccttc
    52321 aaaagtatat gttttaccac taatatattt agtaaaagct gtattttgct cagtagctgg
    52381 agtaatagaa taataactat ctaagaaagc accccagtca tgattatttg tctctaaaaa
    52441 ttcttcaaat tgagttaggc ttttattgct gctgttagat gtagtagttt gagtatcaga
    52501 tgatgagctt tcctgtttgt tattgtcatt tgcctcgttt gatgaggaaa gcgagaaacc
    52561 tacggtagta aaaataatcc cgataataag gagaatcata ggtgttttct tatttgtttt
    52621 tttgataaat cttacaatta acatgattaa acctataata aataaaacaa taccagtaaa
    52681 accaaatata ccgtccatta atagatcctc tttctctttt gatattgaat attataaacc
    52741 aagcaattct tttttcttaa aatcgaattc ttcttgatta ataatccctt catctaacaa
    52801 ctctttatac tttttaattt catctgcact tgaaatttgt gtttctttgg aattattatc
    52861 taatatagat tgttgcttct ctatatattt ttttaaattt tccatttcat tccaatattt
    52921 ttttgtaaac ataacggtgt tttcatcttt cgtggccgct aatatacctc cgcgactttc
    52981 gtttccgcct agcaaggtaa attgtatata accattactc atacctggtt tttttaattg
    53041 tacggctgat atatttttaa aggggatagt tttttctcct ttaatacctt gattaactaa
    53101 atttataaat ccttttctaa taattgaaat agaatcatca gttactttaa tcaaggtttt
    53161 tcctggagat tttattaaaa tctctttttc cattttttct tcctcatttc tgtgatatga
    53221 ttttttttgt agaagctcat atatgaggaa agagcccgtg ttgcagcacg ggctttttta
    53281 atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc
    53341 gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt
    53401 attctgaata gaataatact ccataaattt ttcgagatta aactgagatt catcagtcag
    53461 ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc
    53521 aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa aatgagaggc
    53581 gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcac cgtatagatt
    53641 ttcattcaaa aaaatgattc tgggttttgg atagtagaaa ccaggctctt tcatctccat
    53701 ataaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaaac
    53761 catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt
    53821 gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc
    53881 acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga gacatcgaaa
    53941 tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat tatttttcca ctttgctaga
    54001 taaccgttag aatatccaaa attaatttct aactgccgta tagatatctt tttcctcttc
    54061 gccaagtctt ttattatttc ataggtattc atcgataaaa caacctttct aaatgtttac
    54121 aaaaaaagtt tagaaaaata cgcagaaata atttgactaa ttctgagtaa tgagctatac
    54181 tatatcttgt aaacagattt aacaactaaa aagacaacaa aaaacactat tgatatgtaa
    54241 atgcagaccg ccaagaaagc tttaaaatca atgtttttat gtcttattta attatgctct
    54301 tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg ttaaatttgt
    54361 ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa
    54421 caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt
    54481 gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa
    54541 gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac
    54601 gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa
    54661 cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca
    54721 gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta
    54781 aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa
    54841 atctcctatt tagatagcat attatcttct acagattcag taacaattag tcagattgca
    54901 gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag
    54961 aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa
    55021 tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa
    55081 tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct
    55141 caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa
    55201 tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt
    55261 acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg
    55321 gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc gattatcact atgtacgaat
    55381 tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa aatcccaatt ccagaaggct
    55441 atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat
    55501 ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta
    55561 gagttttaaa gaacccaaga tatagaaata ttatcgatat cgagaaaaac aaaaatggat
    55621 ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg
    55681 aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat
    55741 agagcgctag tgttcagcgc accgctaatc atctacgctt taggactttg gggaagcagg
    55801 caagcgttga taggaacgat tgtttacatg gtttggattt ttatggggct tgatgaagct
    55861 gaagctgagt acagagcgaa aaagccaacc gagggggctg actaaaaatg aatagaaaag
    55921 agaaactaga atgtatatta ctattactca gtttaattct ttcactaatt tctctattgg
    55981 gaagtttgta tttttgatat caacaatttt gtggatttgg gttctattta gagtaaatat
    56041 catttttgtt tggtgattaa ggagtttatt aaaattaatt ttccctactt caaatgcaag
    56101 gtaacaagat atagcacttt taggaggaat atttagcggt acagcttcag agtatattct
    56161 attatcatcg actgatcctc ctttagcaat taatttctta aatggagtag ctgaccatct
    56221 gcggtttaag ttagcggatg aaagttctaa attaactaac atagctggtt ctgttgaata
    56281 gttagaaatg attacttttg tataaatcat atctgcagcg aagtaagaag cattaagttc
    56341 aacttctagt tgagggcgca ttccttttat ttttatccct gttaaaaacg tactcaatat
    56401 aaaacttatt attgacatcc attgaaaaat tgttaagtga aaaaaattca aaataaacac
    56461 caccagtttt taactaaatt ataccaaaaa ggagagaaga aataatgcaa gaattagtaa
    56521 ttttgaaaaa taaagaagct gtgactacga gcttacaagt ggcagaaagc ttcgagaaaa
    56581 aacatcaaca tgttttaaga gatattgatg cactaaaaaa agatgtgtcc aattttggac
    56641 agatgtttgt agaaggtaat gaaccagatt catatggcag aaatcgacga gttttcttca
    56701 ttagtagaga tggatttttc ttgctggcta tggggtttac aggaaagaaa gctatctact
    56761 tcaaacaaaa atacattgaa gcattcaacg aaatggaaga tgttattcgc aagaatactg
    56821 ttcctcaaac aattgaagat atgatgatct atcaattaga agaaatgaaa gatgttaaaa
    56881 aagatgtttc catgcttaaa gatactatgc gaattagcgg acaacaagag tttgaaatta
    56941 agcaaaaagg aaatatgaaa gttatgggag ttctaggggg
//
LOCUS       DACBZN010000007        56347 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.007, whole genome shotgun sequence.
ACCESSION   DACBZN010000007 DACBZN010000000
VERSION     DACBZN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.007"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            290..1135
                     /locus_tag="IVD64_000430"
     CDS             290..1135
                     /locus_tag="IVD64_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6147706.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IVD64_000431"
     CDS             1416..2636
                     /locus_tag="IVD64_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP6147707.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IVD64_000432"
     CDS             2633..5353
                     /locus_tag="IVD64_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6147708.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IVD64_000433"
     CDS             5337..6281
                     /locus_tag="IVD64_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6147709.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IVD64_000434"
     CDS             complement(6350..7360)
                     /locus_tag="IVD64_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6147710.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IVD64_000435"
     CDS             complement(7540..7884)
                     /locus_tag="IVD64_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6147711.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IVD64_000436"
     CDS             complement(7877..8311)
                     /locus_tag="IVD64_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP6147712.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IVD64_000437"
     CDS             8449..9186
                     /locus_tag="IVD64_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147713.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IVD64_000438"
     CDS             9179..10390
                     /locus_tag="IVD64_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147714.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IVD64_000439"
     CDS             10597..11376
                     /locus_tag="IVD64_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP6147715.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCNNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IVD64_000440"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IVD64_000440"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP6147716.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IVD64_000441"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IVD64_000441"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP6147717.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IVD64_000442"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IVD64_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP6147718.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IVD64_000443"
     CDS             14978..16027
                     /locus_tag="IVD64_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP6147719.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IVD64_000444"
     CDS             16024..16302
                     /locus_tag="IVD64_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP6147720.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IVD64_000445"
     CDS             16277..16747
                     /locus_tag="IVD64_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP6147721.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IVD64_000446"
     CDS             16764..17066
                     /locus_tag="IVD64_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147722.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IVD64_000447"
     CDS             17083..17454
                     /locus_tag="IVD64_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP6147723.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IVD64_000448"
     CDS             17426..17785
                     /locus_tag="IVD64_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147724.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IVD64_000449"
     CDS             17957..18964
                     /locus_tag="IVD64_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6147725.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IVD64_000450"
     CDS             18989..20173
                     /locus_tag="IVD64_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP6147726.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTMMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IVD64_000451"
     CDS             complement(20610..22193)
                     /locus_tag="IVD64_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6147727.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IVD64_000452"
     CDS             complement(22771..22914)
                     /locus_tag="IVD64_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP6147728.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="IVD64_000453"
     CDS             complement(23137..23616)
                     /locus_tag="IVD64_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6147729.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="IVD64_000454"
     CDS             complement(23689..23973)
                     /locus_tag="IVD64_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147730.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="IVD64_000455"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVD64_000455"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="IVD64_000456"
     CDS             complement(24317..25603)
                     /locus_tag="IVD64_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP6147731.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="IVD64_000457"
     CDS             25741..26412
                     /locus_tag="IVD64_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP6147732.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="IVD64_000458"
     CDS             26378..26866
                     /locus_tag="IVD64_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP6147733.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="IVD64_000459"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="IVD64_000459"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP6147734.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="IVD64_000460"
     CDS             27827..28591
                     /locus_tag="IVD64_000460"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP6147735.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="IVD64_000461"
     CDS             28824..31037
                     /locus_tag="IVD64_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP6147736.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="IVD64_000462"
     CDS             31048..31494
                     /locus_tag="IVD64_000462"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP6147737.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="IVD64_000463"
     CDS             31665..32924
                     /locus_tag="IVD64_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP6147738.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="IVD64_000464"
     CDS             complement(33013..33960)
                     /locus_tag="IVD64_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6147739.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="IVD64_000465"
     CDS             34354..35646
                     /locus_tag="IVD64_000465"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP6147740.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="IVD64_000466"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="IVD64_000466"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP6147741.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="IVD64_000467"
     CDS             37539..38414
                     /locus_tag="IVD64_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6147742.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="IVD64_000468"
     CDS             38680..40563
                     /locus_tag="IVD64_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6147743.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="IVD64_000469"
     CDS             complement(40638..40922)
                     /locus_tag="IVD64_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6147744.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="IVD64_000470"
     CDS             complement(40912..41646)
                     /locus_tag="IVD64_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP6147745.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="IVD64_000471"
     CDS             41775..42398
                     /locus_tag="IVD64_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP6147746.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="IVD64_000472"
     CDS             42590..43711
                     /locus_tag="IVD64_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP6147747.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="IVD64_000473"
     CDS             43718..46846
                     /locus_tag="IVD64_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP6147748.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="IVD64_000474"
     CDS             46865..50059
                     /locus_tag="IVD64_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6147749.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="IVD64_000475"
     CDS             complement(50205..50912)
                     /locus_tag="IVD64_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6147750.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="IVD64_000476"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="IVD64_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP6147751.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="IVD64_000477"
     CDS             52407..53060
                     /locus_tag="IVD64_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP6147752.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="IVD64_000478"
     CDS             53286..54284
                     /locus_tag="IVD64_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP6147753.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="IVD64_000479"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="IVD64_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP6147754.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18537 a         9724 c        11679 g        16407 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaataatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaatgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcac
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 atttttt
//
LOCUS       DACBZN010000008        54446 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.008, whole genome shotgun sequence.
ACCESSION   DACBZN010000008 DACBZN010000000
VERSION     DACBZN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.008"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..588
                     /locus_tag="IVD64_000480"
     CDS             <1..588
                     /locus_tag="IVD64_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147755.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="IVD64_000481"
     tRNA            complement(828..911)
                     /locus_tag="IVD64_000481"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            1294..2511
                     /locus_tag="IVD64_000482"
     CDS             1294..2511
                     /locus_tag="IVD64_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6147756.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="IVD64_000483"
     CDS             2695..3531
                     /locus_tag="IVD64_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6147757.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     NEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="IVD64_000484"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="IVD64_000484"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP6147758.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4976..6034
                     /locus_tag="IVD64_000485"
     CDS             4976..6034
                     /locus_tag="IVD64_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP6147759.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6053..7072
                     /locus_tag="IVD64_000486"
     CDS             6053..7072
                     /locus_tag="IVD64_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP6147760.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7174..7890)
                     /locus_tag="IVD64_000487"
     CDS             complement(7174..7890)
                     /locus_tag="IVD64_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6147761.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7859..8644)
                     /locus_tag="IVD64_000488"
     CDS             complement(7859..8644)
                     /locus_tag="IVD64_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6147762.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8635..9384)
                     /locus_tag="IVD64_000489"
     CDS             complement(8635..9384)
                     /locus_tag="IVD64_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP6147763.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9682..10206
                     /locus_tag="IVD64_000490"
     CDS             9682..10206
                     /locus_tag="IVD64_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147764.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10222..11721
                     /locus_tag="IVD64_000491"
     CDS             10222..11721
                     /locus_tag="IVD64_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP6147765.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11809..12411)
                     /locus_tag="IVD64_000492"
     CDS             complement(11809..12411)
                     /locus_tag="IVD64_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6147766.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12515..13471
                     /locus_tag="IVD64_000493"
     CDS             12515..13471
                     /locus_tag="IVD64_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP6147767.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13776..14732)
                     /locus_tag="IVD64_000494"
     CDS             complement(13776..14732)
                     /locus_tag="IVD64_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6147768.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFVSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14906..15466
                     /locus_tag="IVD64_000495"
     CDS             14906..15466
                     /locus_tag="IVD64_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147769.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15459..16283
                     /locus_tag="IVD64_000496"
     CDS             15459..16283
                     /locus_tag="IVD64_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP6147770.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16293..16910
                     /locus_tag="IVD64_000497"
     CDS             16293..16910
                     /locus_tag="IVD64_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP6147771.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="IVD64_000498"
     CDS             16928..17611
                     /locus_tag="IVD64_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6147772.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="IVD64_000499"
     CDS             18294..19577
                     /locus_tag="IVD64_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6147773.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="IVD64_000500"
     CDS             19593..20480
                     /locus_tag="IVD64_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6147774.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="IVD64_000501"
     CDS             20497..21333
                     /locus_tag="IVD64_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6147775.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="IVD64_000502"
     CDS             21336..21959
                     /locus_tag="IVD64_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147776.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="IVD64_000503"
     CDS             21956..23248
                     /locus_tag="IVD64_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6147777.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="IVD64_000504"
     CDS             23820..24401
                     /locus_tag="IVD64_000504"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP6147778.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="IVD64_000505"
     CDS             24404..25711
                     /locus_tag="IVD64_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP6147779.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="IVD64_000506"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="IVD64_000506"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP6147780.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26599..27732
                     /gene="purK"
                     /locus_tag="IVD64_000507"
     CDS             26599..27732
                     /gene="purK"
                     /locus_tag="IVD64_000507"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP6147781.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="IVD64_000508"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="IVD64_000508"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP6147782.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWVELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="IVD64_000509"
     CDS             complement(29220..29597)
                     /locus_tag="IVD64_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147783.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="IVD64_000510"
     CDS             29853..30581
                     /locus_tag="IVD64_000510"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP6147784.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="IVD64_000511"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="IVD64_000511"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP6147785.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="IVD64_000512"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="IVD64_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP6147786.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="IVD64_000513"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="IVD64_000513"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP6147787.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="IVD64_000514"
     CDS             33716..35155
                     /locus_tag="IVD64_000514"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP6147788.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35187..36230
                     /locus_tag="IVD64_000515"
     CDS             35187..36230
                     /locus_tag="IVD64_000515"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP6147789.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="IVD64_000516"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="IVD64_000516"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP6147790.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="IVD64_000517"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="IVD64_000517"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP6147791.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="IVD64_000518"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="IVD64_000518"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP6147792.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="IVD64_000519"
     CDS             39749..40663
                     /locus_tag="IVD64_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP6147793.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40796..42361)
                     /locus_tag="IVD64_000520"
     CDS             complement(40796..42361)
                     /locus_tag="IVD64_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6147794.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42354..43805)
                     /locus_tag="IVD64_000521"
     CDS             complement(42354..43805)
                     /locus_tag="IVD64_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6147795.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43819..44835)
                     /locus_tag="IVD64_000522"
     CDS             complement(43819..44835)
                     /locus_tag="IVD64_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6147796.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45055..46143
                     /locus_tag="IVD64_000523"
     CDS             45055..46143
                     /locus_tag="IVD64_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6147797.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46197..47723
                     /locus_tag="IVD64_000524"
     CDS             46197..47723
                     /locus_tag="IVD64_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP6147798.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47725..48930
                     /locus_tag="IVD64_000525"
     CDS             47725..48930
                     /locus_tag="IVD64_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6147799.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49187..50164
                     /gene="guaC"
                     /locus_tag="IVD64_000526"
     CDS             49187..50164
                     /gene="guaC"
                     /locus_tag="IVD64_000526"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP6147800.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50428..51852
                     /locus_tag="IVD64_000527"
     CDS             50428..51852
                     /locus_tag="IVD64_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6147801.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52313..53422
                     /gene="pdhA"
                     /locus_tag="IVD64_000528"
     CDS             52313..53422
                     /gene="pdhA"
                     /locus_tag="IVD64_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP6147802.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53425..54402
                     /locus_tag="IVD64_000529"
     CDS             53425..54402
                     /locus_tag="IVD64_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP6147803.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17403 a         9315 c        11921 g        15807 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaatcgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcaa cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     4981 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5041 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5101 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5161 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5221 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5281 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5341 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5401 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5461 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5521 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     5581 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     5641 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     5701 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     5761 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     5821 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     5881 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     5941 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6001 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6061 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6121 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6181 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6241 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6301 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6361 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6421 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6481 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6541 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     6601 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     6661 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     6721 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     6781 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     6841 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     6901 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     6961 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7021 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7081 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7141 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7201 cccattcttt gtcaattcat aacaggttag cggccggccg atttccaaat atttgattct
     7261 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7321 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7381 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7441 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7501 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7561 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     7621 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     7681 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     7741 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     7801 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     7861 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     7921 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     7981 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8041 gcgtaaatat tttttctgag atttcttctg atatcccgac accgtcatcg cagacggtca
     8101 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8161 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8221 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8281 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8341 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8401 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8461 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8521 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     8581 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     8641 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     8701 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     8761 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     8821 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     8881 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     8941 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9001 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9061 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9121 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9181 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9241 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9301 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9361 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9421 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9481 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9541 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
     9601 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
     9661 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
     9721 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
     9781 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
     9841 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
     9901 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
     9961 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10021 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10081 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10141 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10201 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10261 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10321 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10381 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10441 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10501 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10561 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    10621 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    10681 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    10741 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    10801 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    10861 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    10921 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    10981 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11041 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11101 atcgtagaac gtcaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11161 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11221 gcacaagctc aaaaagtcaa agaagtgact gaagccgaag cagaacgatt ccgtgtggaa
    11281 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11341 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11401 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11461 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11521 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    11581 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    11641 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    11701 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    11761 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    11821 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    11881 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    11941 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12001 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12061 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12121 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12181 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12241 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12301 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12361 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg ccaaattgca tagagattct
    12421 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12481 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12541 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    12601 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    12661 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    12721 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    12781 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    12841 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    12901 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    12961 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13021 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13081 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13141 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13201 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13261 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13321 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13381 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13441 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13501 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13561 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    13621 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    13681 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    13741 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    13801 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    13861 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    13921 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    13981 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14041 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14101 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14161 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14221 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgacgaaa
    14281 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14341 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14401 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14461 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14521 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    14581 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    14641 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    14701 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    14761 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    14821 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    14881 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    14941 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15001 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15061 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15121 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15181 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15241 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15301 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15361 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15421 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15481 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15541 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    15601 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    15661 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    15721 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    15781 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    15841 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggtag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40861 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40921 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40981 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac
    41041 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    41101 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    41161 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    41221 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    41281 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    41341 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    41401 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    41461 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    41521 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    41581 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    41641 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    41701 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    41761 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41821 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41881 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41941 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    42001 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    42061 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    42121 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    42181 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    42241 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    42301 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    42361 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    42421 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    42481 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    42541 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    42601 ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg
    42661 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    42721 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42781 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42841 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42901 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42961 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    43021 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    43081 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    43141 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    43201 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    43261 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    43321 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    43381 ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg
    43441 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    43501 ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata
    43561 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    43621 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    43681 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    43741 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43801 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43861 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43921 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43981 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    44041 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    44101 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    44161 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    44221 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    44281 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    44341 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    44401 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    44461 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    44521 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    44581 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    44641 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    44701 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    44761 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44821 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44881 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44941 ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt
    45001 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    45061 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    45121 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    45181 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    45241 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    45301 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    45361 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    45421 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    45481 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    45541 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    45601 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    45661 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    45721 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45781 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45841 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45901 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45961 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    46021 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    46081 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    46141 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    46201 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    46261 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    46321 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    46381 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    46441 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    46501 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    46561 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    46621 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    46681 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    46741 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46801 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46861 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca
    46921 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46981 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    47041 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    47101 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    47161 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    47221 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    47281 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    47341 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    47401 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    47461 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    47521 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    47581 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    47641 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    47701 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    47761 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47821 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47881 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47941 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    48001 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    48061 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    48121 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    48181 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    48241 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    48301 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    48361 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    48421 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    48481 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    48541 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    48601 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    48661 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    48721 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48781 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48841 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48901 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48961 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    49021 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    49081 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    49141 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    49201 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    49261 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    49321 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    49381 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    49441 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    49501 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    49561 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    49621 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    49681 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    49741 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49801 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49861 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49921 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49981 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    50041 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    50101 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    50161 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    50221 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    50281 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    50341 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    50401 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    50461 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    50521 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    50581 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    50641 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    50701 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    50761 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50821 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50881 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50941 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    51001 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    51061 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    51121 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    51181 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    51241 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    51301 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    51361 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    51421 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    51481 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    51541 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    51601 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    51661 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    51721 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51781 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51841 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51901 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51961 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    52021 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    52081 gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    52141 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    52201 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    52261 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    52321 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    52381 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    52441 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    52501 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    52561 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    52621 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    52681 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    52741 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52801 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52861 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52921 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52981 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    53041 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    53101 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    53161 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    53221 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    53281 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    53341 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    53401 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    53461 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    53521 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    53581 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    53641 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    53701 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    53761 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53821 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53881 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53941 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    54001 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    54061 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    54121 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    54181 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    54241 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    54301 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    54361 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    54421 aaagtcatgg cttatcaatt taaatt
//
LOCUS       DACBZN010000009        54407 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.009, whole genome shotgun sequence.
ACCESSION   DACBZN010000009 DACBZN010000000
VERSION     DACBZN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.009"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            156..464
                     /gene="rpsJ"
                     /locus_tag="IVD64_000530"
     CDS             156..464
                     /gene="rpsJ"
                     /locus_tag="IVD64_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP6147804.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            495..1124
                     /gene="rplC"
                     /locus_tag="IVD64_000531"
     CDS             495..1124
                     /gene="rplC"
                     /locus_tag="IVD64_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP6147805.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1152..1775
                     /gene="rplD"
                     /locus_tag="IVD64_000532"
     CDS             1152..1775
                     /gene="rplD"
                     /locus_tag="IVD64_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP6147806.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1775..2065
                     /gene="rplW"
                     /locus_tag="IVD64_000533"
     CDS             1775..2065
                     /gene="rplW"
                     /locus_tag="IVD64_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP6147807.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2104..2937
                     /gene="rplB"
                     /locus_tag="IVD64_000534"
     CDS             2104..2937
                     /gene="rplB"
                     /locus_tag="IVD64_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP6147808.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            2980..3258
                     /gene="rpsS"
                     /locus_tag="IVD64_000535"
     CDS             2980..3258
                     /gene="rpsS"
                     /locus_tag="IVD64_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP6147809.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3281..3628
                     /gene="rplV"
                     /locus_tag="IVD64_000536"
     CDS             3281..3628
                     /gene="rplV"
                     /locus_tag="IVD64_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP6147810.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3642..4298
                     /gene="rpsC"
                     /locus_tag="IVD64_000537"
     CDS             3642..4298
                     /gene="rpsC"
                     /locus_tag="IVD64_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP6147811.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4301..4735
                     /gene="rplP"
                     /locus_tag="IVD64_000538"
     CDS             4301..4735
                     /gene="rplP"
                     /locus_tag="IVD64_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP6147812.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4725..4913
                     /gene="rpmC"
                     /locus_tag="IVD64_000539"
     CDS             4725..4913
                     /gene="rpmC"
                     /locus_tag="IVD64_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP6147813.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            4938..5204
                     /gene="rpsQ"
                     /locus_tag="IVD64_000540"
     CDS             4938..5204
                     /gene="rpsQ"
                     /locus_tag="IVD64_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP6147814.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5262..5630
                     /gene="rplN"
                     /locus_tag="IVD64_000541"
     CDS             5262..5630
                     /gene="rplN"
                     /locus_tag="IVD64_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP6147815.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5667..5975
                     /gene="rplX"
                     /locus_tag="IVD64_000542"
     CDS             5667..5975
                     /gene="rplX"
                     /locus_tag="IVD64_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP6147816.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6003..6542
                     /gene="rplE"
                     /locus_tag="IVD64_000543"
     CDS             6003..6542
                     /gene="rplE"
                     /locus_tag="IVD64_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP6147817.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6561..6746
                     /locus_tag="IVD64_000544"
     CDS             6561..6746
                     /locus_tag="IVD64_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP6147818.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6782..7180
                     /gene="rpsH"
                     /locus_tag="IVD64_000545"
     CDS             6782..7180
                     /gene="rpsH"
                     /locus_tag="IVD64_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP6147819.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7212..7748
                     /gene="rplF"
                     /locus_tag="IVD64_000546"
     CDS             7212..7748
                     /gene="rplF"
                     /locus_tag="IVD64_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP6147820.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            7909..8265
                     /gene="rplR"
                     /locus_tag="IVD64_000547"
     CDS             7909..8265
                     /gene="rplR"
                     /locus_tag="IVD64_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP6147821.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8286..8786
                     /gene="rpsE"
                     /locus_tag="IVD64_000548"
     CDS             8286..8786
                     /gene="rpsE"
                     /locus_tag="IVD64_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP6147822.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8801..8980
                     /gene="rpmD"
                     /locus_tag="IVD64_000549"
     CDS             8801..8980
                     /gene="rpmD"
                     /locus_tag="IVD64_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP6147823.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9024..9464
                     /gene="rplO"
                     /locus_tag="IVD64_000550"
     CDS             9024..9464
                     /gene="rplO"
                     /locus_tag="IVD64_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP6147824.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9464..10759
                     /gene="secY"
                     /locus_tag="IVD64_000551"
     CDS             9464..10759
                     /gene="secY"
                     /locus_tag="IVD64_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP6147825.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10821..11468
                     /locus_tag="IVD64_000552"
     CDS             10821..11468
                     /locus_tag="IVD64_000552"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP6147826.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11659..11877
                     /gene="infA"
                     /locus_tag="IVD64_000553"
     CDS             11659..11877
                     /gene="infA"
                     /locus_tag="IVD64_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP6147827.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            11910..12026
                     /gene="rpmJ"
                     /locus_tag="IVD64_000554"
     CDS             11910..12026
                     /gene="rpmJ"
                     /locus_tag="IVD64_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP6147828.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12044..12409
                     /gene="rpsM"
                     /locus_tag="IVD64_000555"
     CDS             12044..12409
                     /gene="rpsM"
                     /locus_tag="IVD64_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP6147829.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12437..12826
                     /gene="rpsK"
                     /locus_tag="IVD64_000556"
     CDS             12437..12826
                     /gene="rpsK"
                     /locus_tag="IVD64_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP6147830.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12906..13844
                     /locus_tag="IVD64_000557"
     CDS             12906..13844
                     /locus_tag="IVD64_000557"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP6147831.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13872..14252
                     /gene="rplQ"
                     /locus_tag="IVD64_000558"
     CDS             13872..14252
                     /gene="rplQ"
                     /locus_tag="IVD64_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP6147832.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14524..15363
                     /locus_tag="IVD64_000559"
     CDS             14524..15363
                     /locus_tag="IVD64_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6147833.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15339..16208
                     /locus_tag="IVD64_000560"
     CDS             15339..16208
                     /locus_tag="IVD64_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6147834.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTADELAD
                     AIVHYAGGNAHDE"
     gene            16198..16995
                     /locus_tag="IVD64_000561"
     CDS             16198..16995
                     /locus_tag="IVD64_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6147835.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17198..17944
                     /gene="truA"
                     /locus_tag="IVD64_000562"
     CDS             17198..17944
                     /gene="truA"
                     /locus_tag="IVD64_000562"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6147836.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18277..20946)
                     /locus_tag="IVD64_000563"
     CDS             complement(18277..20946)
                     /locus_tag="IVD64_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6147837.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(21184..21372)
                     /locus_tag="IVD64_000564"
     CDS             complement(21184..21372)
                     /locus_tag="IVD64_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP6147838.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21642..22004
                     /locus_tag="IVD64_000565"
     CDS             21642..22004
                     /locus_tag="IVD64_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6147839.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            22056..23738
                     /locus_tag="IVD64_000566"
     CDS             22056..23738
                     /locus_tag="IVD64_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP6147840.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            23855..25891
                     /gene="recG"
                     /locus_tag="IVD64_000567"
     CDS             23855..25891
                     /gene="recG"
                     /locus_tag="IVD64_000567"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP6147841.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            25940..26941
                     /gene="plsX"
                     /locus_tag="IVD64_000568"
     CDS             25940..26941
                     /gene="plsX"
                     /locus_tag="IVD64_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP6147842.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            27063..27305
                     /gene="acpP"
                     /locus_tag="IVD64_000569"
     CDS             27063..27305
                     /gene="acpP"
                     /locus_tag="IVD64_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6147843.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            27573..28577
                     /locus_tag="IVD64_000570"
     CDS             27573..28577
                     /locus_tag="IVD64_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147844.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            28578..29522
                     /locus_tag="IVD64_000571"
     CDS             28578..29522
                     /locus_tag="IVD64_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147845.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            29522..30484
                     /locus_tag="IVD64_000572"
     CDS             29522..30484
                     /locus_tag="IVD64_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147846.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            30511..31425
                     /locus_tag="IVD64_000573"
     CDS             30511..31425
                     /locus_tag="IVD64_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147847.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            31451..33232
                     /locus_tag="IVD64_000574"
     CDS             31451..33232
                     /locus_tag="IVD64_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6147848.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            33580..34266
                     /locus_tag="IVD64_000575"
     CDS             33580..34266
                     /locus_tag="IVD64_000575"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6147849.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            34286..37867
                     /gene="smc"
                     /locus_tag="IVD64_000576"
     CDS             34286..37867
                     /gene="smc"
                     /locus_tag="IVD64_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP6147850.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            37876..38685
                     /locus_tag="IVD64_000577"
     CDS             37876..38685
                     /locus_tag="IVD64_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6147851.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            38696..39694
                     /gene="ftsY"
                     /locus_tag="IVD64_000578"
     CDS             38696..39694
                     /gene="ftsY"
                     /locus_tag="IVD64_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP6147852.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            39881..42148
                     /gene="gshAB"
                     /locus_tag="IVD64_000579"
     CDS             39881..42148
                     /gene="gshAB"
                     /locus_tag="IVD64_000579"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP6147853.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(42237..42689)
                     /locus_tag="IVD64_000580"
     CDS             complement(42237..42689)
                     /locus_tag="IVD64_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP6147854.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            42839..43789
                     /locus_tag="IVD64_000581"
     CDS             42839..43789
                     /locus_tag="IVD64_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6147855.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            43782..44741
                     /locus_tag="IVD64_000582"
     CDS             43782..44741
                     /locus_tag="IVD64_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6147856.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            44738..45493
                     /locus_tag="IVD64_000583"
     CDS             44738..45493
                     /locus_tag="IVD64_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6147857.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            45516..46469
                     /locus_tag="IVD64_000584"
     CDS             45516..46469
                     /locus_tag="IVD64_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6147858.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(47113..47409)
                     /locus_tag="IVD64_000585"
     CDS             complement(47113..47409)
                     /locus_tag="IVD64_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP6147859.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            47764..48120
                     /locus_tag="IVD64_000586"
     CDS             47764..48120
                     /locus_tag="IVD64_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6147860.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            48130..49029
                     /locus_tag="IVD64_000587"
     CDS             48130..49029
                     /locus_tag="IVD64_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP6147861.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            49545..50276
                     /locus_tag="IVD64_000588"
     CDS             49545..50276
                     /locus_tag="IVD64_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6147862.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            50421..52448
                     /locus_tag="IVD64_000589"
     CDS             50421..52448
                     /locus_tag="IVD64_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP6147863.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            52556..53653
                     /locus_tag="IVD64_000590"
     CDS             52556..53653
                     /locus_tag="IVD64_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6147864.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            53960..>54407
                     /locus_tag="IVD64_000591"
     CDS             53960..>54407
                     /locus_tag="IVD64_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6147865.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        18168 a         9627 c        11677 g        14935 t
ORIGIN      
        1 ttactttagt gaattgcaca gacgtaatca gaatgatttc gtcggctaag aaacgagagg
       61 ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac ggaaattttc gtggagctat
      121 gtctactttt caaaataggc gaaggaggga acaagatggc aaaacaaaaa attcgtatcc
      181 gtttaaaagc gtatgaacac cgtattttag atcaatcagc ggataaaatc gtggaaactg
      241 caaaaagaac tggagctagc gtgtctggtc caattccatt accaactgaa cgcagtcttt
      301 acacaatcat ccgtgcgact cataaataca aagattcacg cgaacaattc gaaatgcgta
      361 cacacaaacg tcttatcgac attgtgaacc caacaccaaa aactgttgat gctttgatga
      421 agctagactt accatctggt gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata
      481 atggaggtgt actcatgacc aaaggaatct tagggaaaaa agtgggaatg acacaaatct
      541 tcactgaatc tggtgaatta attccagtaa ctgttgtaga agctactcca aacgtagtac
      601 tacaagtgaa aacaatggaa acggatggct acgaagctat tcaagttggt taccaagata
      661 tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca aacacggctc
      721 ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag
      781 aaattacagt agatgttttc caagcaggag acattattga tgtaacaggt actacgaaag
      841 gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag ccgcggacca atggctcacg
      901 gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac cgtgtattta
      961 aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa aacttggaag
     1021 ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt cctggagcta
     1081 aaaaatcttt aattactatc aaatcagctg tgaaagctaa ataattgtag gaagaaagga
     1141 ggaactaagg aatgccgaat gtagcattat tcaaacaaga tggaagccaa aacggtgaaa
     1201 tcacacttaa cgaagaaatc ttcggaattg aacctaatga aagtgttgtt tacgatgcaa
     1261 tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg
     1321 ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat
     1381 ctatccgctc accacaatgg cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt
     1441 acagctacaa acttcctaaa aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa
     1501 aagttgctga aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa
     1561 aagaattcaa acaagttctt gctaacttat ctatcgactc taaagtattg gttgttctag
     1621 aaaaaggtaa cgactttgct gctttatcag cacgtaactt accaaacgtt tctgtagtaa
     1681 cttctgataa tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa
     1741 ctgctcttac tcaaattgag gaggtgcttg cataatgaac ttactagacg taatcaaacg
     1801 cccagtgatc actgaaaaat caatgcttgc catggatgac aagaaataca cttttgaagt
     1861 ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt ttgacgttaa
     1921 agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc
     1981 aggatataca aaaaaacgtc gcaaagcgat cgttacattg actgaagatt caaaagaaat
     2041 ccaattattc gaagctgctg aataagcact aatagataac tcattaaatt aggagggaaa
     2101 aacgtggcga ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat gacaggttct
     2161 gattttgctg aaatcacaac atcaacacct gaaaaatcat tgttacaacc attgaaaaac
     2221 catgctggac gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg cggacacaaa
     2281 cgccaatatc gcgtgatcga cttcaaacgt aacaaagata acgttgttgc aactgttcaa
     2341 acgatcgaat atgatccaaa ccgttcagct aacatcgcgt tagtacacta tgaagatgga
     2401 gtgaaagctt acatcttagc acctaaagga ctagaagtgg gtatgaaact tgtttcaggt
     2461 ccagaagcag atatcaaagt cggaaatgct cttccattag aaaacattcc agttggtact
     2521 gttatccaca acattgaaat gaaacctggt aaaggtggac aattgatccg ttctgctggt
     2581 acaagtgctc aagtacttgg taaagaaggc aaatacgtat tgatccgctt gaactctggt
     2641 gaagttcgta tgatcttagc tgcatgccgt gctacaatcg gttctgtagg taacgaacaa
     2701 cacgaattaa tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa acgcccaact
     2761 gtacgtggta gcgtaatgaa ccctaatgat cacccacacg gtggtggtga aggtaaagct
     2821 cctatcggac gtaaagcacc agtttctcct tggggtcaac cagctcttgg ttacaaaact
     2881 cgtaacaaaa aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa taaataatta
     2941 ctttgaccat gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag tttaaagaaa
     3001 ggaccttttg tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag
     3061 aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt tgttggatat
     3121 accatcgcag tttatgatgg acgtaaacat gtaccagttt acatccaaga agacatggta
     3181 ggacacaaat taggtgaatt tgcacctaca agaacttatc gtggacatgc tgctgacgat
     3241 aagaaaacga aacgctaatt tgaggggagg ataaatccaa atggcagaac aaatcacatc
     3301 agctaaagca actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt tagtaattga
     3361 ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca caccaaacaa
     3421 atctgctgga atcattgaaa aagtattgat gtcagcagtt gcgaatgcag aaaataactt
     3481 tgacttagat gttgaaaact tggtagtaag cgaagctttt gttaacgaag gacctacaat
     3541 gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca atcaacaaac gtacaagtca
     3601 tatcacggta gtcgtatcag aaaaataagg agggataatc agtgggtcaa aaagtacatc
     3661 caattggaat gcgtgtaggc atcatccgcg actgggatgc taaatggtat gctgaaaaag
     3721 agtatgctga gttcttacac gaagatttaa gaatccgtaa atttatttca actaaacttg
     3781 ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa
     3841 ttcacacagc taaaccaggt atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa
     3901 gaaaagaatt aaacaaatta actggtaaaa gaatccatat caatatcgtg gaaatcaaaa
     3961 aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg
     4021 ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag
     4081 ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat
     4141 actcagaagg aactgttcct cttcatactt tacgtgcgga cattgattac gcatgggaag
     4201 aagcagatac aacatacgga aaactaggag ttaaagtgtg gatctatcgt ggagaaattc
     4261 ttccaactaa aaaaaacact gagaaaggag ggaaataatc atgttagtac ctaaacgtgt
     4321 aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt
     4381 agcattcggt gaatacggtt tgcaagctgt tgattcacat tggatcacaa accgccaaat
     4441 cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag tatggattaa
     4501 aattttccct cacaaatctt atactgccaa agcaattggg gtacgtatgg gttctggtaa
     4561 aggggcacct gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc
     4621 aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca aattaccaat
     4681 gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa
     4741 tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt
     4801 ttaatcttag attccaatta gcaacaggtc aattagaaaa cactgcacgt attaaagaag
     4861 tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga acaagctaag taattatgga
     4921 aggaggccat taagcgtatg actgaaggaa gaaatcaacg caaagtttat caaggtcgcg
     4981 tggtttcaga caaaatggat aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc
     5041 ctatctatgg caaacgcatg aactattcta agaaatacaa agtgcatgat gaaaacaaca
     5101 cagcaaaagt tggcgacatc gtgagaatta tggaaactcg tccattatca gctacaaaac
     5161 gtttccgttt gttggaagta gtcgaagaag cagttattat ctaataacac gagattttcc
     5221 tatatagatt gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc
     5281 gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg
     5341 gatctggtcg taaaactgct aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa
     5401 cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat
     5461 caggagctcg tcgtacagac ggttcatata ttaaatttga tgaaaatgct gcagtgatta
     5521 ttcgtgatga taaaagccct cgtggaacac gtatctttgg gccagttgca cgtgaactac
     5581 gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga agttttataa tcttgagaca
     5641 cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa gttaaagtta
     5701 tcactggaaa agacaaaaac aaagaaggcg ttgtactagc agcgttcccg aaaaaagata
     5761 aagttattgt cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct caagctgctc
     5821 ctcaaggtgg aattgttgag atggaagcgc caattcatgt ttctaacgtg atggtggttg
     5881 actctactgg tgtagctggc cgagttggct acaaagaagt agacggcaaa aaagtccgtg
     5941 tttctaaaaa aaccggtgaa gttttagata aataaaagtt taaggaagga gggtcttact
     6001 gaatgaaccg cctaaaagaa aaatatctta aagaagtaac tccatcattg atggaaaaat
     6061 ttaactatag ctctgttatg caaacaccta aagttgaaaa aatcgtcatc aacatgggtg
     6121 ttggtgatgc ggtatcaaac gctaaaaact tagataaagc agttgaagaa ttaactttga
     6181 tcactggtca aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga ttccgtttac
     6241 gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg agaaagaatg tacgaattct
     6301 tagacaaatt ggtttctgtt tcactaccac gtgtacgtga cttccatggt gtaagtaaaa
     6361 aagcctttga cggtcgtgga aactatactt taggtattaa agaacaattg atcttcccag
     6421 aagtagatta tgatttagta gataaagtac gtggtatgga catcgttatt gtaacaacag
     6481 caaactctga tgaagaatct cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat
     6541 aattaaagga ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg
     6601 caaaattttc aacacaagct tatactcgtt gcgaacgttg cggacgtcca cattcagttt
     6661 atcgtaagtt ccatctttgc cgtatttgct tccgcgaact tgcctataaa ggtcaaattc
     6721 ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca taagaaggag gtaaaagatc
     6781 aatggtcatg acagatccaa ttgcagattt tctaactcgt atccgtaacg caaacatggt
     6841 taaacatgag tctttagaag tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt
     6901 gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa gatgacaaac aaggcgtgat
     6961 ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat
     7021 ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg
     7081 tttaggtatc gcaatcattt cgacttctga aggtgttgtc actgataaag aagctcgtga
     7141 aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa tctacaagga
     7201 ggtgtcttta agtgagccgt atcggaaata aagtcgttgt tatccctgaa ggagtaacag
     7261 ttactcaaga cggaaacaac gttacagtta aaggaccaaa aggtgaattg actcgtactt
     7321 tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt aactttcact cgtccaaacg
     7381 acagtaaaga aatgaaaaca attcatggaa caactcgtgc gaacttcaac aacatggttg
     7441 taggtgtcag cgaaggtttc caaaaagcgt tagaactaat cggggttggg taccgtgcac
     7501 aattacaagg atcaaaactt gtattgaacg ttggttactc acatccagta gaaatcacac
     7561 caccagctgg agtaactgtt gaagtaccag ctaacacaca agtaattgtt aaaggtgcga
     7621 acaaagaaga agttggcgaa ttagctgcaa acattcgtgg aactcgtcct ccagaacctt
     7681 ataaaggtaa aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg
     7741 gtaaataatc gtttagaaaa attgcataga agccagcagc aggaaaaagg catttggctg
     7801 aagtttacct tcaaaaaaat acatttttac ttgtgccgca gcttctattt ttcgaaacgt
     7861 atttactgct tgaaatagta gtacaataaa aataaagagg tgacaattgt gattacaaaa
     7921 ccagataaga acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt
     7981 actgctgagc gcccacgctt gaacgttttt cgttctaaca aaaacatcta cgctcaaatt
     8041 attgatgacg tagcgggtgt aacgctagca agtgcctcta ccttggataa agaaatttca
     8101 ggtggaacaa aaacagaagc agcacaagct gtcggtaaac tagttgctga acgtgcagct
     8161 gaaaaaggca ttaaagtagt agtctttgac cgtggtggat acctttacca tggccgtgtg
     8221 caagctttag ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa ggaggaatac
     8281 cattcatggt ttatatcgat ccaaaacatt tggaattaga agaccgcgtt gttgcaatta
     8341 accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct ttagttgttg
     8401 tcggtgacaa aaacggacac gtagggtttg gtacaggtaa agctcaagaa gttcctgaag
     8461 caatccgtaa agcaattgaa gatgcgaaga aaaacttagt tgaagtacct atggttggtt
     8521 caacaatccc acacgaagta atcggtgctt ttagtggtgg tagaatccta atgaaacctg
     8581 ctgtagaagg ttctggggtt gctgctggtg gaccagttcg tgccgtatta gaattagctg
     8641 gggtagcaga tatcacttct aaatctttag gctcaaatac accaatcaac gttgttcgcg
     8701 caacagttga aggtttgaaa caattgaaac gtgcagaaga agttgcagaa cttcgcggca
     8761 aatcagttga agaattaatc ggataaggag gacaacaata atggctgaat taaaagttac
     8821 tttaaaacgc agtattatcg gacgtcctca aaaccaacgc gatacagtta aagcgttagg
     8881 tcttacaaag attaacagtt cagtggttaa gccttctaat gaagcaatta aaggcatgat
     8941 caatactgtt tctcacctag tggacgttga agaaatttaa tttagaaaaa tcataactag
     9001 aacattaagg aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct gaaggttcac
     9061 gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa actgccggac
     9121 gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga tttgaagggg
     9181 gacaaacacc attgttccgt cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag
     9241 actatgcagt cattaatctt gatgtattga accgctttga agacggagct gaagtaactc
     9301 ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa agctggaatc aaagtattag
     9361 ctaatgggga actaaacaag aaattaactg tgaaagcagc aaaattctct aaagcagctc
     9421 aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta gactattaaa
     9481 agatgctttt aaagtaaaag acattagatc gaaaatccta tttactgtat ttgtgttgtt
     9541 tgtatttcgc ctaggtgcac atattactgt accaggcgta aatgctgctt cattgcaaaa
     9601 cctttcttct ttgccatttt tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa
     9661 cttttccatt ttctcaatgg gggtttcacc atacattact gcttctatcg tcattcagct
     9721 tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg
     9781 taaaaaactg aatcaagcga ctcgatatct gacattagta ttggcattta tccaatctgt
     9841 cggtatcact gctggattca atcaatacgc tcaattaggt ttcgtgaata atcctaacgt
     9901 agcaacttac gtaacgatcg gtttgattct aacagccggt acgatgtttg ttacttggct
     9961 aggagaacag atcacagaaa aaggtatcgg aaatggcgta tcaatgatca tttttgcagg
    10021 tatcatttct agattgcctg aaagtatcag agaaatttat gaagattact ttataaatat
    10081 tgatccttca gacatatgga aatctgctat ttttgtggcc attttactag tagcggtctt
    10141 gttgatcgtg acattcgtaa catttttcca acaagctgaa cgcaagatcc cgatccaata
    10201 tacgaaacgc gtagctggcg cgcctacaag cagctatctg ccgttgaaag taaatgcagc
    10261 tggagttatt ccagttatct ttgctagttc gtttattgct acgccaaatg ctgttttaca
    10321 agcattgagc ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa
    10381 ctataataca gcacctggtg ctacgattta tacgattctg atcgttgcct tcactttctt
    10441 ctacgccttt gttcaagtaa accctgagaa attagcggaa aacttgcaaa agcaaggaag
    10501 ttatatacca agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa gattactgat
    10561 gcgtttaagt acagttggtg cagtcttcct tggtttagtg gcattgcttc caatcattgc
    10621 tcaaatggtt tgggatcttc cacaatcaat cggattaggc ggtacaagct tactgatcgt
    10681 tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata
    10741 tgttggattc attgagtaag aaagcgtgtt gaggtttccc ctcaacacac gttctcacac
    10801 atttattagg agggaaaaca atgaacctca ttttaatggg gctgccaggc gcaggtaaag
    10861 gaacacaagc tgaaaaaatc attgatgctt atggtattcc gcacatctca acaggagata
    10921 tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag tcttatatgg
    10981 ataaaggtgc attagttcct gatgaagtaa caaatggaat cgtgaaagag cgactagctg
    11041 aaccagatac agagaaaggc ttcttattag atggctttcc acgtacgcta gatcaagcaa
    11101 aagctctgga cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc atcgatatcc
    11161 atgttggtga agaaatcttg gtcgaacgtc tagccggccg atttatctgc agcaattgtg
    11221 gagcaactta ccataaaatt ttcaacccta caaaagttga agacacatgt gatcgttgcg
    11281 gtgggcatga attctatcaa agagaagatg ataaacctga gacggttaaa aatcgtctgg
    11341 ctatcaacat caaaaatagt gaaccaatct tggcttatta taaagaacaa ggtttgctaa
    11401 acactattga tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg
    11461 aaaaatagca cgattgcccg tagccgactt gcattatgca gttactcttg tcaagttcaa
    11521 taaattatga tagaatatgt cagtccgact tctagagaat cctctagatg atccattttg
    11581 agaattttga ggtgggaact gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag
    11641 tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc
    11701 gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa acggacacca agttcttgct
    11761 actgtttcag gtaaaattcg tatgcattac atccgtatct tacctggtga caaagtaacg
    11821 gttgaattgt ctccatacga tcttaaccgt ggccgtatta cttatcgctt taaataattg
    11881 tactccgtaa aactacagga ggtattatca tgaaagtaag accatcagta aaaccaatgt
    11941 gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc
    12001 caaaacataa acaacgtcaa ggataattgg aggtgctata aatatggctc gtattgcagg
    12061 agtagatatt cctcgtgata aacgtgtagt agtttcactt acttatatct atggtattgg
    12121 taacactact gctaaaaaga tcttagctga tgctggtgta tctgaagatg ttcgcgttcg
    12181 tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa gtagataaat taaaagttga
    12241 aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa tcggttctta
    12301 ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga aaaacaatgc
    12361 acgtactcgt aaaggcccag ctcgcgctat cgctggcaag aaaaaataat tcttaagtga
    12421 aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa
    12481 tatagaagca ggtattgcac atatccattc tacattcaat aacactatca tcatgatcac
    12541 tgatactcac ggaaatgcat tagcgtggtc atcagctggt tcattaggtt tcaaaggaag
    12601 caaaaaatca actccttttg ccgctcaaat ggcagcagaa actgctacaa aagcagcaat
    12661 ggaacacgga ttaaagactg ttgaagtaac agttaaagga cctggttctg gacgtgaagc
    12721 agcaattcgt tcattacaag ctacaggttt agaagtgact gcaattcgtg acgtgactcc
    12781 agttcctcat aatggtgccc gccctccaaa acgccgtcgt gtttaatgag tgccgctcat
    12841 tttgagtttt atcagtaacg tcattgaaca agacactttt cgttttgaaa ggggtaaaga
    12901 taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg
    12961 gcaagttcat cgttgaacct cttgaaagag ggtatgggac tactttaggg aattccctac
    13021 gtcgtatttt attatcttct ttgccaggtg ctgccatcac taatattcaa atcgatggtg
    13081 tactgcatga attctctacc atcaaaggtg ttagagaaga cgtcactcaa atcatcttga
    13141 acattaaagg cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata
    13201 ttaccggacc tgctacagta actgctggcg atattatcgt tgatagtgat gtagaaattc
    13261 ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa
    13321 cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa
    13381 ttggcgttct tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg
    13441 agaacacacg tgttggacac cgtgaagatt ttgataaatt gacgatggag atttggacag
    13501 atggttcaat tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg actgaacatt
    13561 tggatatttt tgtgaactta actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag
    13621 aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga agagttagac ttgtctgttc
    13681 gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt acaaataaat
    13741 ctgagcctga aatgatcaaa gtccgtaact taggacgtaa atcattagaa gaagttaaag
    13801 caaaattgca cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga
    13861 gggaaaccaa cgtgagttat cgtaaactag gacgcacatc tagccaacgt aaagcgatgt
    13921 tgcgtgattt aacaactgat ttattaatca acgaacgtat tgtgactact gaagctcgtg
    13981 cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc
    14041 atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa
    14101 atgaggacat cgtcattgaa tcagctttac aaaaactgtt caacgatatt gcacctcgtt
    14161 acgcagaacg tcaaggcgga tacacacgca ttttgaagac agaaccaaga cgcggtgatg
    14221 gtgcaccaat ggttgtctta gaacttgtct aaaatcacac taataacatc actttataga
    14281 tgattctttt agagtgttat gatgttggaa gatatttttc cgagtctagc tcagcgctgc
    14341 tcctcaagcg aatatcgctt ggggagtaag cactcatcat aaagtgtttt tttatacctt
    14401 caattacctg tcatgacaga tatgtttgaa ggcataaaaa aacattttaa gcgagaaata
    14461 attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac tttaggatga agaaggagta
    14521 atcatggagc caattattga attagaaaag ataaattata aataccagcc agatgatctt
    14581 cgccccgcat tgaaagatgt ctcctttaca attgataaag gtgagtggat cgctattatt
    14641 ggacacaatg gttcaggaaa atcgacactt gccaaaacga tcaatggatt gcttctgcca
    14701 gaatcaggga tcgtgaaagt gggaaatcaa atattggatg aagaaaatat ttggacgatc
    14761 cgacagatgg ttggaatggt ttttcaaaat ccagacaatc agtttgttgg ttcgacagta
    14821 gaagatgacg ttgcttttgg tcttgaaaat caagggattc ccagagaaga aatgctagtg
    14881 cgtgtgaaag atgcacttga gaaagttaga atggctgagt ttgcttcacg cgagcctgct
    14941 cgtttatcag gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc cttacgacct
    15001 gatatcatta ttttagatga agcaaccagt atgctagatc cagaaggtcg tgaagaagtg
    15061 atctctacga tcaagaagat caaagaagaa agtcaattga ccgttatatc tatcactcat
    15121 gatattgatg aagcagctaa tgccaatcgg atactagtga tgagacaagg cgaacttgtc
    15181 cgtgaaggta caccaaaaga aattttttct gctggtcctg aactgatcga cttaggtcta
    15241 gatctaccat ttcctgaaaa actgaagagc gcactgaaag aacgtggggt tgatgtgccc
    15301 agtgaatata tgacagaaga aaggatggtg gattggttat ggacatccgt tttgaacaag
    15361 tagacttcac ttatcagccg aatacaccat ttgaacaaag agccttattt gacatcaata
    15421 tgacgatcaa agaaaacagt tatactgcat tagtagggca taccggaagc gggaaatcaa
    15481 ccttacttca gcatttgaat gcgcttgtta agccaacaag cggtacagtc catattggag
    15541 aacgagatat tcagccggat acggataata agaatttaaa gcccatccga aaaaaagttg
    15601 gcatcgtctt tcagtttcca gaagcacagc tattcgaaga aacggtagca aaagacattg
    15661 ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa
    15721 cattagaaca agttgggctg gatgaaagct atttggaacg ttcgccattt gaactttcag
    15781 gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg
    15841 tattggatga accaacggca ggacttgatc cccagggcag aaaagaaatg atggagatgt
    15901 tttggcgtct tcataaggaa cagcagatca ctattgtatt agttacgcat ttaatggatg
    15961 atgtagccaa ttttgcagat tatgtctatg tgttggaaaa gggacgaatc gttaatagcg
    16021 gggagccgca agaggtcttt caaaacattg aatggctaaa agaaaaacaa ctaggcgtac
    16081 ctactgccac cgagtttgca gaagaattaa tggctaaagg gatgaacttt gctaccttgc
    16141 ctttgacagc tgatgaatta gcagacgcta tcgtccatta tgctggagga aatgcccatg
    16201 atgaataaat tgatatttgg tcgttatatg cctggagatt ccttgatcca tcgattggat
    16261 ccccgagcta aattattagc cagtttttat tttatcggaa tcattttttt agcaaataat
    16321 tgggaaacgt accttttgtt ggcatttttc acattacttt ctgtcttctt gtcaaaagta
    16381 gatttgaaat tttttatccg aggcattcgc ccattgatct ggctaatcct cttcactgta
    16441 gctttgcagg tcttgtttac aagtggcgga gaaacttatt ggagttgggg tgttttccat
    16501 atcactgaat ttggtgtgat gaacggcctc tttattttct gtcgattcgt gttgatcatt
    16561 tttatgtcga ctctactcac gcttactaca ccgcctttgg aattatcgga tgcgattgag
    16621 tacttgcttc gtccattgaa agccgtacgg ttcccagtcc acgaaatttc attgatgctg
    16681 tcgattgctc ttcgttttgt gcctacattg atggacgaaa cagaaaagat catgaacgct
    16741 cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc
    16801 attcctttgc tgattccttt atttgtcagc agcttcaacc gtgcggaaga tcttgcgact
    16861 gctatggaag caagaggcta caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat
    16921 tggcataata gagacacgat ggttatcatt gcttttgtat tattaactgt gctactgatc
    16981 tttttaagag gataaacgtt cagtgggccg ctatctattc ttttcttgct gatctgtttg
    17041 caagaaagga tgcaagcagt gtatgacaag aaatattagg gtctgtggca gactaaggtc
    17101 acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa attttataga aaaatcatta
    17161 ttccagataa ccttttagga acaaaggaga gaaaaacatg gtacgttaca aagcaatcat
    17221 tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag ccaaatgggc gtactgttca
    17281 agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg
    17341 ttcgggaagg acagatgccg gagtacatgc gatcgggcaa gtaatccatt ttgattatcc
    17401 agaggagcgt ccgttagaac gaatgcgttt cgcgctagat acccagtcac cagaggatat
    17461 tgctgtaaga caagtagaaa ttgtttcaga agacttccac gcaagatatt tggtcaaaga
    17521 aaagacgtat caatttcgag tggatatagg gaaaccacgc agtccttttc gacgccatta
    17581 tgcaagttat tttccttacc cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt
    17641 attagggact cacgatttca cctctttttg tgcatcagga agctcgatag aggataaaat
    17701 acgtacgatc tacgaggcta agatggaggt aaatgaaaca aaagacgaat tgttgtttac
    17761 atttagggga aatggcttct tgtataagat gatacggatc ttagtaggaa cgcttttgaa
    17821 aattggcaat ggccgattgc ctgaagatag tatcccagat attattgcga aaaaagatcg
    17881 taatgcagca ggacctacag cacaccctga aggactttat ttgtatgaag tgaaatatga
    17941 ataagaggtt atgatagaag tggtcagctt caagaaataa gaaggtattt acgaaaatta
    18001 cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga agctacttct ggaacaccgt
    18061 ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaaagagca atttcaaaaa
    18121 acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat aagtgaatca
    18181 tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc
    18241 ttggtgcgga cagtctgaac aggttccatc gtttctttat tttctacgtc ttcttctgtg
    18301 tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg
    18361 gtcaaggtac cagacataaa gaataaacaa tgcaatacat aggacaaaaa ggattgcacc
    18421 tatcgtaaat ccttcaggca agtaactgaa agtgacagta tgtttacctg ctggaacttt
    18481 tatcatcaag aatgcgtctt tgaaattgct gaccttgact ggtttcccat caattttagc
    18541 tttccatcct ttgtcataag gaatagttgt caccaacatg gaatcttcag cggtatttac
    18601 agtcccagaa gcttttctgc cagtggcttc catttcgaca cctttttctt tgatctgatt
    18661 cattgcggct tgataggcta caacatctag accaacgact ttaggctcca tgaaacttat
    18721 ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc
    18781 aagatcatag tattgaccag aaatgttgat ttgcgttttt cgcgatgtac cattgacagt
    18841 gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc
    18901 ttgtgtattt gcaggcacat ggacttccca agtgagctct tttggctggt tatctgctgt
    18961 ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa
    19021 agtaggtgta tagaatgtaa aatactgttc atttagacca gacaatgtat tgatcaagtt
    19081 tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc
    19141 agtaaatgct agtggcaatg cattgacatt ctgatacagt gtatagtcgc cgctttgacc
    19201 agcttggcgg aagccatatt tactgaacgt gctttcgttt ttagaaatgt tatatttgat
    19261 tcctgtaaac ccatccatca gcaaagtatt attgttataa cggatattca aatttgtccc
    19321 tcttgaacgg aaacctaatt tatccaggta agaggaagaa ttacgatttc ttatagaaga
    19381 aaacaaactg ataccactgt aaccatagtt gatcgaatcg tttgcagata ctggatccag
    19441 attttccaag cggtaaaacg agtcggaatc tttttttgtt tcatttacaa gagctttcaa
    19501 gtcaggataa ggatcagtat ataaactgcg ggaagcataa ttccaatcat caagaatgcc
    19561 atggatcata ctgttcgtat tgaccagtgc ttcagcagac atgatcaata ataacaatat
    19621 actcagatga ttgaatgaaa gttttttatg ctgataaaat cccacagcaa aagcataaat
    19681 ccctaagaaa accactgtca atacaaagga agtcatcgtc acataggtgt agcttccttc
    19741 aggtttgatg ccccatgcca ccgcaaagag agcagctacg accaaaatga ttcctaacaa
    19801 cagcccacga tcgtcttgtt ctatcttttc ccatcctttt cctgcaagca ggagcactaa
    19861 gaaagagaac agataggcat aacggaataa aaacatattc ggtgcatgca taccatgcca
    19921 gaacaaattc aatggaacaa gataaaaact tgcgataagt atggcgaaaa gactgccgta
    19981 caaaaattta tttttcaacg gtactttccg actggcaaaa taaaacagac agaggactaa
    20041 tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat tttgtcgtgt cataaacacc
    20101 aatcatattt ttcatgatta gatccagata agaagtcgct tctgttttca acgtagtgat
    20161 ctgactcagt gcctcgccat ttgaccgaag atccagcact gctggcaata caatgatcat
    20221 tgaagcgccg cctgccagta aagaagtgat accataagga acaatcgttt ttttatattc
    20281 tgaccagttt gtcaatagac gggcgataaa atataagaaa gaaaatacgc caatcataaa
    20341 acccatataa aaactggata aaaacaagag taaataactg ataaataaaa gtttcggccg
    20401 tttaagatcc ataacacgat gaatccccaa aatcactaat ggaagataaa caaatgcatc
    20461 cagccacatg atgatctccg aatgtgcagt gataaatgag atcaatgcgt aactgacgct
    20521 aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa
    20581 tccggcacag ccgaccttaa gcaaagtcag aacatacagt gcatctggca tcatctggtt
    20641 tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa
    20701 ataattcaat cctagagaag cattccatgt ataaaaaata ctttgctttc caagaagaac
    20761 atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact
    20821 tctgctgctt ccaggataga ttccgatact tagataaaca agtgctaaaa tcaaaaacgg
    20881 aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta aagaatgact ttaaattttt
    20941 tttcatattt attttccacc aaatttctat gaagttgtat tcagtttatc atatccttgt
    21001 ttttctctgc ttaaaaagag tctagttttc acaaaaaaat aaaatttaga ataaataact
    21061 tcataatttg ttcgtataac aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca
    21121 cccttaaaaa tttttattaa ataaaaaacc gttcactatc tgaacggtcc aagtatcatt
    21181 caattaaacg cgttcaactt ttcctgattt caaagcacga gttgacaccc aaactttttt
    21241 aggttttccg tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt
    21301 tgagttcata gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg taaagtaaca
    21361 aacttttgcc atggtaattt cctcctttgc cttgatttgg agcaatagaa taattcagct
    21421 cataccagac taacatatca tatagaatgg gaatttgcaa gtacattttc atagatacaa
    21481 ttgacaattg agaagaataa ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt
    21541 ttttgagtac tttgggtaac tgccttttat ttcaagttgc gctatgataa aatatgatag
    21601 aagaaaaatg ggagtttttt acccgttaag gaggctcatt catggctgta aaaattcaaa
    21661 cgtcagctgg aaccattgaa attaccaatg atgtaattgc aacagttgtt ggtggcgccg
    21721 cgacagatgt gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta
    21781 ctgagatttt gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa gaagataatg
    21841 gtattgctgt agatgtttat acgattatca gctatggtac gaaaatctct gaagtttccc
    21901 gcaacgtcca agaaaaagtg aaatacaatc tagaggcatt attaggcgta actgccaatt
    21961 cagttaatgt ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga gactggctgg
    22021 taactcagca gtcgatctga aggaggatta tctaggtgag tgtaacagaa ataagtgcaa
    22081 gccagttcca ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg
    22141 tcaattcatt aaatgtattc cctgtgccag atggcgatac gggaacaaat atgaatctat
    22201 ctatgactag tggagctaag gcagtagtag attcaacctc agaaaaagta ggagaattag
    22261 cagcaatgct ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt
    22321 ctcaattgtt tcgtggattt tctaagcaaa ttccagatgt tgtctcatta aatgcttctg
    22381 atcttgctgc agccttcact catggagttg aaactgcgta taaagcagta atgaaaccag
    22441 tagaaggaac gattttgaca gtagctagag aagcagcgaa agctggagaa aagaaagcaa
    22501 aaagtactga tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca aagcgtgctt
    22561 tagctaaaac tccagacttg cttccagtcc taaaagaagt cggtgtcgta gacagtggag
    22621 ggcaaggact gctgtttgtt tatgagggct ttttaagtgc attgaatgga gaattccaag
    22681 cggatgaaac atatgaacct tctccagcag aaatggatga tatggtcaat gcagaacatc
    22741 atcgcagtgt ccaaggtcaa atggcgacag aagatatcca ttttggttac tgtacagaaa
    22801 tcatggtaaa aatcggagaa ggacctacag tcgatagtac attcgattac gaagaattcc
    22861 gtaactattt agatggtata ggagattctt tacttgtggt caatgatgac gagatcatca
    22921 aagtccacgt gcatacagaa aacccaggag aagtcttgaa ttatggacaa aaattcggtt
    22981 cattagtcaa aatcaaagtc gacaacatgc gcttgcagca tgagacgatt ttggaacacg
    23041 atgaagaagt gaacgagttt gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag
    23101 cgatcgctgc tggtaaaggc gtccaagagc tgtttgaaag tttaggcgct aattatgtta
    23161 tcagtggtgg acaaacaatg aatccaagta cggaagacat tgttaaagcg attcaagaag
    23221 taaacgcaga taaagtgatt gtcttgccaa acaataagaa catcttcatg gcagctgatc
    23281 aagcagcaga agtagcagat atcccagtag cagttgttcc ttcaagaacg atttcccaag
    23341 gattgacagc tatgctttca ttcaatgatc aagcttcttt agaagaaaac aaagaaacga
    23401 tgacagaaat gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc agagatacct
    23461 caattgacgg tgtagagatc aaagaaggag actttttggg catgatcgat ggaaaaatcc
    23521 tgatctctaa cccagatatc acagcaacag cgttaagtac actagaacaa atgatcaatg
    23581 aggacacaga gatcgtaacg atcttggcag gtgatgaagg aacagttgaa caagcaaatg
    23641 aatttgctga gaaactaaca gaaaaacatc ctgatttgga aatcgagatc caccaaggag
    23701 atcagcctgt ctatccttat ctattttctg ctgaataatt tctgtgtcag aatatctttc
    23761 taacacagta ttcattcata aaaggccttc ggttagtgat caggtatgat cgctgaccaa
    23821 gtcctttttt tgttaaagga gggaggaaaa atttatgcgt tctttagatg atccaatcac
    23881 tatgctggca ggagtcggag aaaaaagagc agctagtctt gccagcttgg gcatccaaac
    23941 gattgaagac ttactgactt attatccttt tcgctacgaa gatattcagg aaagaaatct
    24001 aaacgagatc caggatcaag aaaaggttac tttgaaagga atcgtagtct cgcctcctgt
    24061 gatgaatcga tttggttata aaaaaagccg attgcaattt cgtatgatgc aagaacatga
    24121 tgtgttcaac gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga
    24181 ggaaatcgcc gtatacggta aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat
    24241 tttaggttct caaacaatgg atgacttttc gccgatatat cacgtcaaca aatcaattcg
    24301 tcaaacgaca ttagttgatc taatcaaaaa agggtttcaa gaatacggca cattgattga
    24361 agaaaatctg ccaaacaacc ttgtggagaa atatcgactg cttgaccgag cttctgctgt
    24421 aagaagtatg cattttccaa aagaccacga agaaaaccat caagccaaac gacgtgtcgt
    24481 ttttgaagag ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc
    24541 agaaacgaat ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa
    24601 gctacctttt gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct
    24661 tcgcagtccg cagcatatgc agcgactttt gcagggagat gtaggtagcg gaaaaacagt
    24721 ggttgccgcg attgcgttat acgctaccgt tacagcaggt ttccaaggtg cgttgatggt
    24781 gcctacagaa atcttggcac aacaacatat ggaaagcttg caacagctgt ttgatcccaa
    24841 tgaagtaacc atcgcattga ttacggggtc aacgaaagca aaagaacgac gtgagctcct
    24901 tgaaaaacta gagcaagggg aaattaacgt cgtgatcggc acacatgcat tgatccaaga
    24961 tggagtcgaa tttcagaatt taggtctagt tattacggat gagcagcacc gttttggcgt
    25021 gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc
    25081 gactccgatt cctagaacat tagctattac agcatatggt gagatggatg tatctatcat
    25141 tgatgaactt ccagctggac gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt
    25201 ggacagtgta ctggattgga cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat
    25261 ctgtccgctg attgaagaat cagaagcatt agatgtaaaa aatgcagtgg aaatctatga
    25321 aaaacttcgt gaactatttg caccaaaata tgaagttggc ttgttacatg gtaaaatgaa
    25381 gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac aaccagatgc agatattagt
    25441 ttcgactact gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat
    25501 ggatgcggat cgatttggat tggcacagct gcaccaactt cgtggacgtg ttggacgcgg
    25561 aagtgatgct tcctactgta tcttagttgc taatcctaaa aatgaactag gtgtggaacg
    25621 gatgaaaatc atgacagaga ccaccaatgg ttttgtacta agcgaaaaag atctggaatt
    25681 acgtggtcct ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc
    25741 tgacttggtg acggatggaa atgttttgga ggtagccaga gaagaggctg cagccctttg
    25801 gcaaataaag gattggcaga tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat
    25861 agaaaatcag gataatcgtt atttcgatta gcagactgtg ctatacttta tactgatgac
    25921 ggaaaaagga gtggaaacaa tgaaaattgc cgtagatgca atgggcggag atcacgcacc
    25981 tcaagcaatt gtagaaggcg tggctttagc gcagcaagat tttccagaag tagaattctt
    26041 actttacgga aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa acatcacgat
    26101 tatccataca gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa
    26161 aaagactgca tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt
    26221 attttcagca gggaatacag gcgcgctact agcagcaggg ttgttcatcg taggacgtat
    26281 caaaaatatc gaacgccctg gcttgatgtc taccttgcca gtagtaggga aagaaggtgc
    26341 cggtttcgat atgctggatc ttggcgctaa cgcagaaaac aaaccagaac atttgttgca
    26401 atacggtatt ttaggttctt tttatgcacg taaagtccga ggtatcggac gtcctcgtgt
    26461 cgcactctta aacaacggaa cagaagcaac aaaaggtagt gaagtgacga aaaaagccta
    26521 tgaactcctt cagaatgaaa caagcttgaa ttttattgga aacgtagaag ctcgtgagct
    26581 actcaatggt gtggctgatg tagtggtcac agatggattt acaggcaacg ctgttttgaa
    26641 atctattgaa ggaacagcaa tgaatatgat gaatctatta aaatctgcta ttctaaatga
    26701 agggatcaaa ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc
    26761 agaaatggat tattccaaac atggcggtgc cgttttgttt ggcttgaaag caccagtcat
    26821 taaaactcat ggagcaacag gccccgatgc tgttcgctat acgatccgcc aaatccacac
    26881 aatgcttgag acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata
    26941 gtatttggat atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat
    27001 gccagtaaaa ttagccatgg tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa
    27061 agttgactcg cgaagaagta tttaacaaag tagcgaaaat tatttcaaat cattttgaga
    27121 tagataccga taaagtgacg gatgaattga acatcaaaga tgatttaaaa gctgattcaa
    27181 ttagtattat ggaattcgtt ttagagcttg aagatgaatt cggtacggaa atttcagatg
    27241 aagacgctga acaaattgaa acagtcggtg gcgcagtaga ttatatcagc agccatctta
    27301 aataatttca aataaaagca aagaaggata aaaagctgca tgcagctttt tatccttctt
    27361 tttttaatct ttttgacacg taaaagtcat ttttctgata attctggcga taaacagaaa
    27421 ttatgttgca aagtaaagcg gttttctata taattattaa taattcatta cacaaagata
    27481 agaataacct ttgttataaa acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa
    27541 cttccattta ggaagcagag aggggtattc gattgtccga taaccaatta ttagaagttc
    27601 agaacctaca tacaggattc cgactcaaag atgactatta cgatgcagta gatgacgttt
    27661 catttacttt agacaaaaac gagattttag caatcgttgg ggaatctggg tgcgggaaaa
    27721 gtaccttggc tactacgatt atgggattac ttgatcctaa taacacaaaa attacaggtg
    27781 agattttata taacgatctg aatttaatcg acctgaacga aacgctctat aataaaatcc
    27841 gcggaaatga tatcggaatg attttccaag atccgttgtc tgcattaaat cctctcatga
    27901 gaatcgaaga ccagatcaaa gaaggacttt cctatcacac aaagatgaat gcagaacagc
    27961 gtcaagctcg tgcactagaa ttgctagaac aagtagggat tcctaatcct gcgagagttg
    28021 gacgccaata tccccatgaa ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg
    28081 caatcgcctg caaacctcca atcgtcattg cagatgaacc gacgacagcc ctagatgtaa
    28141 ccatccaagc acagattctt gatttactga aagaccttca ggaagaaaca aaaacaggga
    28201 tcatcttgat cactcatgac ttaggagtcg tagcagaaat ggctgatcga gtggcagtga
    28261 tgtatggcgg acagttcgtt gaagtagcag gtgtaaaaga actgtttgat catcctaagc
    28321 atccttatac acgctcttta ctgaactcta taccacaaga aggaaatcat gatgcagaac
    28381 tccatgtgat cgaaggtgtc gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt
    28441 ttgcaccacg tattccatgg ataccagcaa gcgatcatga agaagagcct atccttcatg
    28501 aagtcgcacc gaaccacttg gtacgttgct cctgctataa gcatttccac tttagagacg
    28561 aaaaaggaga ggtgtagatg acagaattaa tcacaatcaa agacctgaaa gttcattacc
    28621 ctattcgcag tggattcttc aatcgagtaa cggatcatgt atatgctgta gatggtgtgg
    28681 attttattat cgaaaaagga aagacatatg gtttagtggg agagtctggc tctggaaaat
    28741 ctactacggg aaaagccgtt gtgggattag aaaaggtaac agccggacaa atcatgtatg
    28801 aaggaaaaga tgtaacgaag cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc
    28861 agatgatctt ccaagattca atgtccagtt tgaatcctaa aaagcgagtg ttggatatca
    28921 ttgctgaacc catccgaaat tttgaacggt taagcgatca agaagaaaag aaaaaggtaa
    28981 aaggactgct agatatcgtt gggatgccgg aagatgcgct atataaatat cctcatgaat
    29041 tttcaggcgg acaacgtcaa cgtctaggag tagcccgtgc tgttgctacc aatccaaaac
    29101 tgatcgtagc cgatgaacct gtatctgctc tggatctatc tgttcaagcg caagtattga
    29161 atttcatgaa acgaatccag caagaatacg gcctcagcta tttattcatt tcacatgatt
    29221 taggtgtagt aaaacacatg tgcgacaata tcgcaattat gtacaaaggt cgatttgtag
    29281 aaatcggtac aagagaagac atttataatg acccgcgaca tatctatacg aaacgtctgc
    29341 tttcagctat tccgcaaatc gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa
    29401 gagtagaaca agagtatatc caaaaccaaa aagaatacta tgatgcgact ggtcgagtct
    29461 acgatttgcg tacgatcaca actacacaca aagtggcgtt gaaagatgga ggtgcaagct
    29521 aatgtggaaa acgattctgc gacgtgtttt attgatgatc ccgcaagtta ttattttaag
    29581 tgttctgatc ttcttattag cccaagcgat gccgggggat ccatttaccg gtttgatcaa
    29641 tccaaatcag gatccggcag tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc
    29701 atggtatgtc cagtacttcc gttggatcgg aaatgctttg caaggcgatt ttggtcaaag
    29761 tttcttatac aaacgctctg ttgctgactt gatcggcgaa cggatcatca acactttata
    29821 tctatcgatc ttgacagttg tccttactta tttgattgct gtgcctctgg ggatgcttgc
    29881 tggccggtat caaaattcaa tcttggataa gattgtcgta atctacaact tctttagttt
    29941 tgctgttcct ttatttatct tcggcttgat catgctattt gtttttggtt atcgtttagg
    30001 ctggttccca acaagtggat cacaaacact aggtttgcaa ggtggattta tcactcaatg
    30061 gatagaccgt ttgaaatata ttctgttacc atcaattacc caagcatttt tggcgactgc
    30121 cgtcacaatc caatatctgc gaagtgaagt aatcgattcg aaatcgctcg attttgtcag
    30181 aacagcccgt tccaaagggg taccgacgaa taaagtattc agtcgacata ttttccgaaa
    30241 tgcagcgttg ccgatggctt ctcaaatggg gtatgaaatc acttcgttga ttgctggttc
    30301 tgtagttatc gaaaagattt ttggttatcc gggagtcgga aaactattta ttgattccat
    30361 cggccaacgg gactataccg tgatcacatc cttagtattg attttaggaa tcgcgacgct
    30421 agtggggaca ttactttcag atatcatcat gagtatcgtt gacccgcgta ttcggattca
    30481 ataaaaccaa tcagaaagga gaatggatcg atgagtgaaa aaaacaatac agcagttcaa
    30541 gaaagtattc ctcctatggg gatacgaatg attgctcgag aatttaaaaa agataaaatt
    30601 gctatttttt cattaggttt gctagtggtt ttattattag tgatctttat cggtgcgatg
    30661 ctgaccgatc aagacaaagt gatgtatgtt agtatcttcg acaaatacgc tgcaccagga
    30721 acagtttcga cacaaggaca aaagttcatt ctgggagcag atgaaggcgg acgtgatgta
    30781 ttaggacaat tgatcatagg tgctagaaac tctgttacga tcggatttgc gatcacgatc
    30841 cttacttcga tcatcggggt tggattaggg atcatctctg gctactacgg agggatcatt
    30901 gacaacatct tgatgcggat cgttgatttt atcatgattc taccgatcat gctgatcatt
    30961 atcgtcttcg tatcgattat ttccagatac aatgtctggt cctttatatt catcatgagt
    31021 gcattttatt gggtagctaa agcccgatta ttccgaagca agacattatc tgaagcaaga
    31081 agagattatg tcagtgcctc aaaaacaatg ggaaccaatg attttaaaat catgttccgc
    31141 gaaatcatgc cgaatctaag ttcattaatc attactaatt tgacgatgaa ctttgcggca
    31201 aacatcggta tcgagacaac tttgactttc ttaggtttcg gcttgccggc aaatgtacct
    31261 agtttaggta cattgatcgg ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg
    31321 atctggttac ccgcatcaat ccttatttta gttttgatgt tgagtatcaa ttatgttgga
    31381 caagcattca agcgttctgc agatgcgcgc caacggttag gctagtatag aagcgaaggg
    31441 ggaaatagga atgaagaaaa cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct
    31501 tgcagcatgc gggggcggag gtacagatac ctctggaaac aactctggag gcaacggcaa
    31561 taacaaagag gaattaactt ttccaattgc aacaaaaaat aatgatgatg caatcgaaga
    31621 cggcgaatta gaagctgcgg tcgcaacgga cagtcagttt aaaggattgt tccagtggga
    31681 gttttaccaa gattcatatg atgcagcttt tatggctcct tctcatgaac cgctatttgc
    31741 aaacgatgac gatttcagaa tcacagacga cggagctgca acgcttgatc tagatgaaga
    31801 tacgaaaaaa gcgacaatca aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt
    31861 aacggcggac gatgtgattt tcccttacga aatcattggg aataaagatt acactggtat
    31921 ccgttatgac gacactttcc gtaacatcgt aggtatggaa gaatatcatg atggaagtgc
    31981 cgacacaatc tccggaatca aaaaaataga cgacaaaaca gttgaaatcg aatataaaga
    32041 agtaaaccca agcatgcttc aatcgggtgg tggtatctgg tcatatgcag caccaaaaca
    32101 tacgttagaa ggtgtagcaa tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc
    32161 agtgactttt ggcccttatt acatgagtaa tatcgttgca ggagaatcag tagaatactt
    32221 gccaaacgag cactactgga atggtacgcc taaattgaaa aagatcacga tgaaatctgt
    32281 accgatggca agtgcaactg aagcattgaa ctctaaatta tatgatatga ttttccaaat
    32341 gccaacagat acttacgata cctataaaga aattgctggc tatacaaact taggccgtga
    32401 acaaacttct tatacttatc taggattcaa gttaggtaaa tggaatgcag atacaaagag
    32461 tgtggaatac aatccagatt caaaaatggc tgataaatca ttacgtcaag caatgggcta
    32521 tgcattagat aataatgcag taggtgaacg tttctatgca ggtttgagaa gcaatgcgac
    32581 ttcattgatt cctccagtat tccagtcata ccatgatgat gagatgaaag gattcacaca
    32641 agatctagac aaagcgaaaa aactattaga tgatgctggc tacaaagatg tggatgacga
    32701 tggtttaaga gaagacaaag atggaaataa actaacaatc aactttgctt cgatgtccgg
    32761 tggggaaaca gcgcaaccat tggcagatta ctatatccaa caatggaaag aaattggttt
    32821 ggatgtccaa tacacaacag gtcgtctgat cgaattcaat tctttctatg ataaattaga
    32881 aaacgatgat cctgaaattg atgtttacca agctgcttgg aatacaggaa ctgatccaaa
    32941 tccaagtgga ctatggggac caaatgctgc gttcaactac actcgttatg aatcagaaga
    33001 aaatacaaaa ttgatcaacg atatcagttc aagcaaagca tttgatgaag agtaccaaaa
    33061 agaagcatat caaaaatggc aacaatatgc atttgaagaa gcatttgcga ttccaacgtt
    33121 gttccgtaat gaagtattgc cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga
    33181 tgtaccaaac ccatgggcaa atgtttctgt tacagcagat tcacgttcat aatcactaaa
    33241 aatgaccgaa aaagggcgat gtcctttttc ggttttttga tatttttctg tttatttctc
    33301 taatattcag gtcaaggcgc tgatttcatg acttctccaa taaacggtgt tcaaagtcca
    33361 gtttctcggt attaagtcaa atttatgcaa aaatatggaa agccattttc acaaaatttt
    33421 ttcttcatac tcgaaactaa acgaattttt cacaacttct cgtcaatcac taggattatt
    33481 aaagctttac ttaccttttg accgaaccat ttcaaatttg acaaaaaacc agtataataa
    33541 ggaagtatcg aaaaaatatc cataaaagga atgtggaaaa tggacaacca gctgacaaaa
    33601 gaactaaaag aaaactataa catcgttttt cacgatctaa atttgcttga acaagctttt
    33661 acccattcat cctatgtgaa tgagcatcgc aatctgcaat tatcggataa tgagcgactt
    33721 gaatttttag gagatgccgt tttagaactg atggtttctc agtatttatt ccgtttatat
    33781 cctcatttgc cagaaggaaa attgacaaaa acacgagctg cgatcgttcg cgaagacagc
    33841 ttgtcgaaat ttgccaaaga atgtcacttt gatcaatatg ttttgttggg caagggagaa
    33901 gagaattcag gaggacgtac gcgaccagca ttgctttgcg acctattcga agctttttta
    33961 ggcgcgttat atcttgacca gggattcgac acgacccatg cattcatcga aaaagtgatt
    34021 tttcctaaag tcaaagcagg tgctttttca catgagatgg atcataaaac aaaactacaa
    34081 gaagtcttgc aaaaatcagg tgatgtatca atcgaatacc gattgatcaa tgaagaaggt
    34141 ccagctcatg aacgagtatt ttggatcgaa gtttatgtag atgatcaatt gattggcact
    34201 ggacaaggga aatcgaaaaa attagcagaa caagctgcgg cggaaaatgc attggcagca
    34261 ctgtaaaagt aagtgaggga aagctttgta tttgaaacga attgaaatag ccggttttaa
    34321 atcatttgca gacaagacag tcattgattt tgaaaatagc gtcacagcag tggttggacc
    34381 aaatggcagc ggaaaaagca atattacaga ggctatccgc tgggtattag gagaacaatc
    34441 tgctaaaagt ctgcgaggcg gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg
    34501 taagcagctg aatattgcag aagtcacagt gattttagac aatagtgatc attatctgcc
    34561 attggagtac aatgagatca gtgtaacccg ccgttatcga cggactgggg aaagcgagtt
    34621 tttcatcaat aaacaaagct gcagattaaa agatatccaa gaattgttct tagattctgg
    34681 attaggaaaa gaatcgttct caatcatttc tcaaggaaaa gtagaagcga ttttcagtag
    34741 taaaccggaa gaccgtcgtg ggatctttga agaagcagct ggtgtcttga aatacaagca
    34801 gcggaaaaag aaagcagaac agaagctttt tgaaacagaa gacaacctca gccgcgtcca
    34861 agacatcatc cacgaattgg aagaacagtt aactcctttg gccgcccaga gtgaggcagc
    34921 aaaggagttt ttacggctaa aagaaacact gactcaaacc gatgtctctt tgatggttgc
    34981 tgaaatcaag acagcaaaaa aagattggga caacaaacaa gcacagcttg cgaaattcaa
    35041 tctagaatta ggcaaactat ctgagtcgat ccaagaacaa gaatccattt tggcgaagca
    35101 gcgtaaagaa aatgcgcaag ccgatcgtct gatcgaaaaa aatcagcaag tattattaga
    35161 tctttcggaa aaactgaagc agacagaagg acaaaaagat gttttgcagg agcggacaaa
    35221 gcatacacaa aaaagctcgc aagaatatca aacttcatta gcagaagcac aaaaaaaggt
    35281 taaacatttt gaaaagcttc aagaaagctt gatgaaagca gcagcagaaa aagaaacaga
    35341 gatccaaaaa gctgaagcaa atctcataaa aacacagcaa gaattagaaa aataccaaaa
    35401 atcaacgaaa gaattattgg cagaattaag agatcaatat gttgatttga tgcaagaaca
    35461 agcagctgta ggaaacgaac tgaagtatct agaacgtcaa tatatacaag aaacagcaaa
    35521 aagcaagcag acgttagcca agcaatcgga agtagaagca tcagtagacc gattgatctt
    35581 acaaaaagaa gaattgacac aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa
    35641 agagaagtta gaaatgatcc aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga
    35701 acagcctaaa atgtatcaat taatgaatca agtccagcag ctacgtgctc gtaagaaaag
    35761 ccttcaagaa atccaagaaa actattttgg tttttaccaa ggggttcgat tggtcttgca
    35821 gcataaacag cagctttcgg gtattgtcgg agcagtggct gaactgatcg atgttcctgc
    35881 agatttcacg ttagctatcg aaacagcttt aggtggggca gcccagcatg tcatcgttga
    35941 aaatgaaaaa gatgctcggc aagccatcac gtatttgaaa cagcaaagag gaggaagagc
    36001 aacattcctt ccactgacga caatcaaacc aagacaatta ccggctcata tattgacaca
    36061 agcagcagct gtagaagggt ttatagggat tgccagtgaa caagtcagtt atcccgatca
    36121 aatccaaact gtggttcaaa acctgctagg aacgatcctt ttagccaaag acttaactag
    36181 tgccaatgca atcgcgcaaa cgatccgcta tcaatatcga gtggtttcct tagaaggtga
    36241 tgtgatgaat gccggaggat ccatgacagg tggagcgaat aaacgaggaa accaaggcag
    36301 tttgtttgta caaaatcaag aattgaaaca gttgactagt gaatttgaag aagcagataa
    36361 acaactccaa gcacaagaaa aaaaagttca ggagctgcaa caagaaacag ctcgtttagc
    36421 cgaagaacaa gaagttttgc gtacacgagg cgaacaatta cgttttgaag aacaagaagc
    36481 gacaaatcag ctacaaaaca tcataaatga acttgaacgt tttgaaaaag aaaagcaaat
    36541 ctcaactttt gagacacgtg aactgcagca gtttatcgaa gattacgaaa agcaaacgaa
    36601 cgaattgaaa gacaaacaaa cagatcttga atcgcaacga caaaaaatcg acgaagaaat
    36661 caaatcgtta agtcaagaaa gcgaccagat ggaagccaga agagcacaag tacaaagtca
    36721 aaaagcacaa gaacaagcag atcttgctgt cttgaaagag cagtttaatc atctgcagat
    36781 ccagctccgc ggtgcccgag tccaaaaagc agaggcaacc gaacgtcaag aagcgataga
    36841 aaagcagttg gcgacattga cagcagactt ttctgatcat gaagtcacag aagaaagttt
    36901 ggaaaaacaa attaacgaat tatctgctca gcgagaaaca ttaaaagccg agttggcaaa
    36961 agccaaagag cagcgagata gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc
    37021 agagcgtaat cgtgagcaaa aagcacgact gtcggaacaa tccaaattag aagtccaaaa
    37081 agaccgagct gaaatgattt tagataatca tttgacctat ctgcagtcag aatatcagct
    37141 gacatttgag aaagcttccc aagactatca agaaacaacc gatatcgaag acagtagaac
    37201 aaaggtttca tccttaaaag aacagattga aaaactggga ccagtaaact taaattcaat
    37261 cgaacaatac gaacaagtca gtgaaagaca tacattctta gcaacacagc gagatgatct
    37321 gttagcagcg aaaaaccaat tgtttgaaac gatggatgag atggatgatg aagtgcggac
    37381 aagattcaaa gaagtatttg aagctatccg tcaagaattc aaaattgtct ttccaaatat
    37441 gtttggcggc ggtaaagctg aattagtact gacggaacca actgatttgc tgaaaacagg
    37501 tattgagatt gaagtacaac ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg
    37561 aggagaacga gcacttacag ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt
    37621 tccgttttgt atcttagatg aggtagaagc agcgttagat gaagccaacg tgaaacgttt
    37681 tggtcgttac ttgagcgatt tccaagatga cacacaattt atcgtagtca cacaccgcaa
    37741 aggaacgatg gtcgcagcag atgtactgta cggagtgacg atgcaggaat ccggtgtatc
    37801 aaaaatcgtt tctgtacgga tggaagatat caatgaagag gcgaaaatca acgaaacgaa
    37861 agtgtaggaa cgaaaatgat caaattagta gcaatcgatt tggacggtac attattaaat
    37921 gaacaaaagc tgatttcaga tgagaataaa caagcattag cacaagcaaa acagcaaggg
    37981 gtcaagatcg ttctatgtac aggacgcccc ttagctgcga tggcgcatta tctgcaagaa
    38041 ttaggactgg tagatgaagg tgactacagc atcactttca acggcggttt ggtacaaaaa
    38101 aatgatactg gtgaaatcat ggaaaagaaa gtgatggaag tatcagatat ccaacgctta
    38161 tacaaactgg ctcagcaatt ggatctgcca ctagatgttt tgtctgacaa tgtggtttta
    38221 cagctgccta gtgccccaag gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa
    38281 ttccaaccag cggatataag cgatattaca gatgaatttg tgcttaataa ggcagtaatc
    38341 gcttatgacc aagaagaatt agatcctaaa atcaaagaaa taccagcaga attccatgaa
    38401 atgtatgaga tcatcaaaac aagaagtatg cttttggaat ttatgccaaa aggtgttaca
    38461 aaagcttatg gtatttcttt attggcaaaa gatcttggtt tgaaacaaga agaaatcatg
    38521 gcgatcggtg atgaggaaaa tgatttaccg atgatccaat atgctggact aggcgtagcg
    38581 atggagaatg ctgtgccttt cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa
    38641 gaaaatggcg tagccaaagt gatccaaaaa tttgtattag agtaggggga gtcaaatggg
    38701 attttttgat aaaatcaagc gtgcttttac tggcgaagag aaaacagaag aagttgaagt
    38761 acaggagaag tacgacaagg gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa
    38821 tgagttgttt gccaattttc gaacagtcga tgaagacttt tttgaagaat tagaagaaac
    38881 acttatcggt gcagatgtag gttttgaaac agcgatacga attaccgaag cattacgtca
    38941 agaagtgaaa ctcagaaatg cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa
    39001 acttgtggat ctctatgaag cggaaggaat caatgaagta aatgaactaa atatccaaga
    39061 agacggacta acagttattt tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat
    39121 tggtaaactt gcccatcagt tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga
    39181 taccttccgc gctggagcaa tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga
    39241 agttgttcgt ggcaatgcag gaggcgatcc agcagctgtc gtattcgatg ctttggaaag
    39301 agcaaagaca gagcaggcag atgttttact agttgatact gcaggccgac tgcaaaataa
    39361 agtgaacttg atgaacgaat tagaaaaaat aaaacgagta atccagcggg aattaccaga
    39421 agcaccccaa gaaacacttc ttgtattaga tgcaaccact ggtcaaaacg cgatggttca
    39481 agcaaaacaa ttcaaagaaa caacagatgt gaccggtctt gttttgacaa aattagatgg
    39541 tacagcaaag ggcggtatcg tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt
    39601 ggtcggtctt ggtgaaggaa tcgatgactt ggagccgttt gatccaaatg attttgtcgt
    39661 tggattgttc aaaggattac taaaagaaga ataacccatg tctaaaaagc aggagctgtg
    39721 acaaaagttg cagctccttg tttgaatgat tgtttttaac atagctgtgt ttttgatgcc
    39781 ttcattttta tacttatttg taagcggttc tcaaacaatt tcgttctttt tcttagactt
    39841 tttgattata attagattca tatgaattgg aggtcactta atgatgaatt ttaagcaatt
    39901 attattgcat gtcaatgcgc gcccttttat cgaccaagct cgatttggta tagaacggga
    39961 aggacagcgg gttgatcttg caggaaatct agcaaaaacc gatcatccag caatctttgg
    40021 cgatcgatcc tatcacccct atatccaaac agattttagc gaaacacaaa cagagatgat
    40081 cacccctgtt accgattcta ttcccgaatt atttcagtat ctcgctgctg tttatgatgt
    40141 gactgctcgt tctataccga aagaagagat gatctggcca ttaagtatgc cacctgcctt
    40201 accggaaaaa gacgaagaga tcattattgc aaaattaaaa aatttcgaag atgtcttgta
    40261 tcgacgttat ttagcaaaag aatacgggaa gcgaaaacag atggtaagcg gtattcattt
    40321 caattttgaa tttggcgatg agttgctaag aacattgttc agccatcagg aagaatttca
    40381 agatttttcc gaattcaaaa cagaacttta tttgaaaaca gcgagaaact ttatgcgcta
    40441 tcgctggatg atcacttatt tattcggtgc ttcaccaatg agtgagaaga actacttttt
    40501 agatgaatca catccgcaag aacctgttcg cagtattcgc aacagtgcgc tgggctatac
    40561 gaatcatcca aatgtgaaag tttcctatgc ttctatgaaa cagtatttag cagatatcga
    40621 gcgaatgatc gaagaaggga aactttcgga ggagaaagaa ttttatacac cgcttcgttt
    40681 ccgaggcgga aagaaagtcg cagatttagc aacaacaggt gttcgttata ttgaattgcg
    40741 gaatatcgat ttgaatcctt atgcaagatt gggtatcaat ccagagcaag ttcggttctt
    40801 acaattattc ctgatgtaca tgttatggac agaagaaaaa gaagactgtg accaatgggt
    40861 agcagaaggt acgactcgaa ataacaaagt agcactagaa caaccatcag atcaaacaga
    40921 atttcatcaa gaaggtagag aaattcttga aggaatgaag caaatgctgg ttgaattgga
    40981 ttggttggat tctctttatt tagtagagga agcattgact caaatggatc atcctgaaca
    41041 aacgttagca gcgaaactct atcaagaagc acagctatcg agtcagcaag aagttgctgt
    41101 ggcattggga catcaatatt ataaagaaag ccatgaacgt ccctatcagt tggccggttt
    41161 tcgcgaaatg gaactttcta ctcagatatt tatgttcgat gccatccaaa aaggtgtcca
    41221 agtcaaagta ttagatgagt cggatcaatt tttgcgtctg caattccaag accatgtaga
    41281 atatgtgaaa aatgcaaaca tgacaagtaa ggacagctat atcgtcccac ttatcatgga
    41341 aaacaaaaca gtaacgaaaa aagtcttaaa agaagctggg ttccgggtac caggaggcgc
    41401 agaattttca tccatggaag aagcggtaaa agcttatccg agatttgccg agcaggcatt
    41461 tgtgatcaaa cctaaatcaa ccaattacgg gttaggtatc acgattttca aggaaggagc
    41521 tagtctggaa gattatcaag caggtttagc cattgctttt cgtgaagaca gttcggtttt
    41581 agtagaagaa tttatgccgg gaacggaata tcgtttcttt gtgatcgacg gagaagtcca
    41641 agccatcatg ttgcgagtcc ctgctaatgt tattggtgac agtatccgaa cagtgaaaga
    41701 acttgttgaa gaaaaaaata gtgacccttt gcgtggaacg aatcatcgtg cacctttaga
    41761 attgatccaa ttaggtgaat tagaacaatt gatgttgaaa gaacaagggt tgacgatcga
    41821 atcagttccc caagccaatc aaattgttta tctaagggag aactcaaata tcagtacagg
    41881 tggtgattcg atcgatatga ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc
    41941 tgcggtagaa gcactaggag ctaagattag tggtatcgat ttgattatac cagataaaga
    42001 aatcgatcct acaactgata aaaaagcata tgggatcatc gaagcaaatt tcaatccagc
    42061 catgcatatg catgtttatc cttttgcagg aaaaggcaga agattgacga tgaatgtatt
    42121 gaaattgtta tatcctgaag tattttaaaa gaaaaagctt ttgacagatt gtcctatttg
    42181 atgtcaatct gatcaaaagc tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa
    42241 aataaacgat cccagaagct ttttttcttt tttggttttt ctttttcagc tggcgaagag
    42301 ttttcttctg ggtatttttt ttcgatatca atcacaggtt catttactgc ttcttttgct
    42361 gttaagagca atccgaattc ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa
    42421 cggctattgc ttttcgttgc tagctggata tattggtttt ggacagaaag gggtagagcc
    42481 ccattcaaga gtatggaagc atctggatgt tttttgagtt cttttaaaaa gttttctttg
    42541 ttttttggtt ggatcatttc agcaacagtc atactaagat agcagcgttc cctgaatgtc
    42601 cctaaatatt tatgctgttc ttcagggttc actaaaggtg taccataaag tcccttatcc
    42661 aaatggcttt ttacatcatc ttgtgccata aaaaataccc tccttcttct ctttgtcttt
    42721 cttacgatta tagcatattc ccaataatac ctaaaacata tgaaaaagcg gttgcttttg
    42781 ttcaacgatt cacttaaaat aagaatgaaa atcattctca attagaaagg tggattggat
    42841 gcgctacgga cgactagtag cattattagt tattttaagt agtatttcat tgttcatcgg
    42901 agtatcgaat gtacaattgt cagaactgct ccagttagat gggcaaagct tacaaatctt
    42961 gctagtgact cgtttgccac gaacgatttg tttgatcctt gtgggagcga ccagtagtat
    43021 ttgtgggttg atcatgcaac atttgaccca aaataaattt gtttcaccaa cgactgcggg
    43081 cacgatggat agtgcgcgtt taggtatttt agtggctatg attttcctac ctggtgcatc
    43141 cttgattgtc cgatccttga cagcattttg ctttgctttt gcaggaacat tgcttttttt
    43201 atcccttacg cgattgttcc tccagaaaaa tcaattgatg cttccgttga ttggagtcat
    43261 gttagggaat atcattggtt cagttgctac tttttttgct tatcaattcc agttggtaca
    43321 aaatatgact tcatggcttc aagggaattt tgcgactgtg atacgaggaa attatgagtt
    43381 attgtatgct gttgttccat tgttgctctt ggtttggcta tttgcttacc gatttacgat
    43441 cattggtctg ggcgaatcgt ttgcagtaaa tctgggaata gattaccaga agatgcagtt
    43501 tttaggcatt ggcttagtat cattggctag tgcagtcatg ttgatcatgg taggaagcat
    43561 tccttttatc ggagtgatca ttccgaatct tgtctctaga atatatggag atcaagtgca
    43621 caaaacaatt ggcgtaactg ctttagcagg tagtttgttc ttagtatttt gcgatcttgt
    43681 cgctcgcgtg ttgatttttc cttatgaaat accagtcagt gtgatcgttg gaatcatcgg
    43741 tggggctatc ttcctttatc ttctgataag gggacgacga tatgcttaaa aaaaagccat
    43801 ctatttttat tggtatgctt gttcttgttt tatttgttac aggaatgtac ttgacgatcc
    43861 atacatatgg aaactggcat tttgcatgtg cattgaggag taagaaaatt tttgcattta
    43921 tccttgtctc gctagctgtc agcagtgcca caatcagctt tcagacaatt acgcataacc
    43981 agtttttgac tccttcagta ttgggacttg agtcgttata tgtccttgtc caaacggtcc
    44041 ttttcttctt tatcggagga gtgacgatgc ttcagcagac aggtacagtt tttttcttat
    44101 tgaacgttgg gctgatggtc gggctgagtc tattgttatt tttctttctt ttgaaaaaaa
    44161 tgagagcgaa tcttttttta ttactgatgt cagggatgat tttaggtacg ctttttggta
    44221 gtgtcagtac ctttttacaa gtcatcatgg atccgaatga atatgatttg cttcaaggca
    44281 gattatttgc aagctttggc aatatccaga gtcagtatct tttgcctgct tctttgatca
    44341 ttatcggctg ctggtacctg ttatttcgat atcgacatgt gctagacgtt ttccatctcg
    44401 gaaatgaaca agcagtaaac ctcgggatag acacagatcg ttttgaattt ttgattttat
    44461 gttgtgtcag tcttttgaca gggacatcaa cggctcttgt gggtccagtt acttttttag
    44521 ggtttattgt cgcaaatatc agttatcgtg tcttttctac ttatcgtcat ggtcagctat
    44581 ttatcggaag ctttctggta gctatgctgt ttctgacagg aggacagctc gcagtagagc
    44641 agattttcgg ctggaataca acagtgagtg tcgtcgttga atttgtcgga ggaatttatt
    44701 ttgtcacaaa attgattcgc gaaaggaaga gtatcctatg attgatttac aaaaggtcaa
    44761 taaactgtac catggaaaat cagtggttaa agatgtacag ttaacgattc ctgaaaagaa
    44821 attgactgct tttatcggtc caaatggtgc tggaaagtca acggtgttgt cgatgatcag
    44881 tcgtttgata ccaaaagata caggtgaaat ctatttagac cataacgaag taaaggcttg
    44941 gaaatccaat gaattagcca aaaaattatc gatcctacga caatcgaatg aaataaatgt
    45001 ccaaatcacc gtgaaagaat tagtagaatt tggacgattt ccttatacga aaggaaaatt
    45061 gacgacaaaa gacgaggaaa tcgttcagga atcactggaa caccttgggt tagaagaaat
    45121 agcggatcaa tcgattgaca ctctttcagg cggtcaactc caacgtgcgt atattgcaat
    45181 ggtgctagcg caagacacgg attatatttt actggatgaa cctttgaaca atctagatat
    45241 gaattttgcg gttcaaatca tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac
    45301 aattgttatt gttcttcatg atatcaactt tgctgcaagt tatgcagacg aaattgttgc
    45361 aatgaaagat ggacagttat ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt
    45421 attggatgaa ttatacgaaa tggacatacg gatttgtgag attgaaggcc gcagattctg
    45481 tctttatttc taaaactaac attaggagag aagagatgaa aaaattagta gtaggtatct
    45541 tgattctagc atcatttggt ttagcagcat gtggaaatag caacaacacc agtcaggcag
    45601 acacaaaaaa aagcagcaca caaactgaaa ctacattgac gataacagat agtaatggag
    45661 accaaatcga ggtgccaaat aatccagaaa aagtcgtcgt attcgacaat ggttctttag
    45721 atacgatgga tgctcttggt gttggagata aggtagttgg agcagctaca agcagccttc
    45781 ctgagtatct ttcatcctat aaaaaagtag aatcagcagg tggaataaaa gaaccagact
    45841 tagaaaaaat taatcagctt cagccagatc taatcatcat ttctggccga caaagagatt
    45901 ttcaaaaaga tctaagtcaa attgctccga ccattttcct ttctttagat gcgaaaaatc
    45961 catgggaatc tttccagcaa aatgtcactg ctttagggga gatctttggt aagcaagaag
    46021 aagctaaaac tcaattagag gaattatcta gtgcaatcga tcagacgaaa aagaaagcag
    46081 aagctactga caaaaaagca ctagtcacac ttgtgaatga agggcaatta tcagcctacg
    46141 gatctggttc tagattcggt ttcatccatg atttatttgg ctttgaacaa gcagatgatc
    46201 aaatcgaagc atccactcac ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc
    46261 cggatatctt atttgtagta gacagaacaa aagctattgg tggagacgac agcaaagatg
    46321 atatttctgc caatgaactg gtagcacaaa cgaatgctgg taaaaatcag cagatcatct
    46381 cccttgaacc agatgtctgg tatttaagcg ggggcggttt agaatcaatg aaactgatga
    46441 tcgaagatgt gaatcaagca tttaaatagc ataaataaca gaggattaaa ctctcatact
    46501 atgtaacaat aatgtttctt acattcttaa atagcaagag cctatcctca aagaagttat
    46561 caaaaaccat ccgtaaaatc gtattgatga ttttacggat ggtttttatt tatttgagaa
    46621 atcttttttc aacgtgaatt cattatctac atcatacggg ttgattctag aatggggagt
    46681 ggcaaaaaat agtgtgatcc attttttcag catcaagatc gcctcctttg atttgaatca
    46741 aaggtacaaa gcgaaactta gataaaactt aaatttagat atttttattt tgtaccgatc
    46801 tcttctttaa acgatgttcc atctgctgct tctcggcaat aagtccacta gactagaaaa
    46861 tatgagaagc tattttctgt ccagcgttct tattactcaa agcaaatcgc agttgtcaca
    46921 atctcttcta aacgatgttc catctgctgc ttctcggcaa taagcccact agactagaaa
    46981 atatgagaag ctattttctg tccagcgttc ttattactca aagcaaatcg cagttgtcac
    47041 aatctcttct aaacaatgtt caatcagctg accttcggta ttagctcaaa gcactgattt
    47101 cacaacctct atttaggcat gtgtctcagc aagcggttga tagttgatga tctgcatttt
    47161 tcgataaaaa tcggcgggaa gcttactcca ccgttttaat aattgataga aataactctc
    47221 ttctgctttg attttatact cgcttaatat caatccaaaa ctattcgttt tttttgaagc
    47281 taatttttta taaatatcca atgctttggt tttatcttca ttgacaaaga gtgtttttac
    47341 ttcaatataa gaaccctcga tattttgtgt ttcttcgata aaatagatgt tttttatata
    47401 ttccttcata gtgattcctc caaagaagtc ttttatttga taagtttact ctaccgaaga
    47461 gttgttaata atccgtgaac aaaaagtaat tctttttcca atagttgtta aaggtttttt
    47521 attttttata gtattttttg ttatttaaga aaggaataag gttaaacaaa taacaggttg
    47581 ctttttgggt ctttttccgt tcaagaaact gaacgaagaa aagggaaagt tgtctctttt
    47641 gtgataagag aaatcataat aaacgaatga cttactttcc aataaattga gaacagtcag
    47701 aaccagacaa attagttgca tctgatgaat aaagtgatta ttctatgaca tgaggtgata
    47761 cagatgaaat tagaagtgac aaaagaagca caagaagtat tgaagaataa attagaagca
    47821 gacgatcaac ttttattaga tatcgaaaac ggtgacggtc catttgcaga aagccaagtc
    47881 agttgccaat tggatacggc atttcgattg atcattgtca aaaaagatac acaaacagat
    47941 ctaagtcttt attctgtagt ggctgataca ccagttggac caatcaaaat caaagacagt
    48001 gcaatacttt acatggatga tccatccaca cttgctttag aaccaactta taatagttta
    48061 caattaaaag gaccaagcgg cttgagaaaa ggcaacttgc aagttgttcg aaaagactag
    48121 agggataaaa tgggaaaatt ggcgatcatc agcgacctac atgtggacat caataaattc
    48181 ggcgaatcag agctgatgca gctttggcaa gttttgcaaa agcacaagat cactcggctt
    48241 catctagccg gagatacggc taataaagta gaccgttgcc tagctgttgt cgaatttttc
    48301 gtcaataaag gattatctac gacatttcat ttcggcaatc acgagttggc agatgtgccc
    48361 aatgaacaga tgatggacca ttttccagac ccacattttt taaatgaacg gtatattgct
    48421 ttaaatgagc agaccgttct tttagggatg aatggctggt acgactatca gttttccgaa
    48481 ttgaaagatt acgaggcaat cgtacgaatg aagcgtttgt attggtatga tcggttcatc
    48541 cagcgtggga aaacagatct cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg
    48601 ctagaccagc tggaagcaaa aaaacttgag gtgattttat ccactcattt cgttcctaaa
    48661 cgggcattta tcatgtatca atctgctcct tatgagcgtt ggaacaaatt aaatgcattt
    48721 ttaggatcag aatcattcgg agaattactt gaccgttatt caaatatcaa acaagtagtt
    48781 tttggccata cgcatcgtcg ctttgaagat aaatccatcg gtcacacgat ttacagctgc
    48841 agaccttttg gttattatta tgaatggcag ctgacccgag attttgtctt gaaagaacag
    48901 ttgctagaca gctatgatcc tatgaaatta cgtgtcctct tgcgtgccca tcacccagct
    48961 tttcaggcct atcaaacaca acatttgcaa gaggaatttg aacaagcgat gacaatcgtt
    49021 ccgtattgag acagcattag taatgtgtaa aagaaaatac cttgtaaaac ataaggataa
    49081 gtaagagcag tagatccatt tatcgtggat atgctgcttt ttttgcggaa aataatcagc
    49141 gaatgtttat ttgatcggat gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc
    49201 tttttttgaa cgataatcac aaaaaaaata taatatacat tttttattta attataatta
    49261 atattgattg aagtctaggc agtcaagtca cattttgata gataggaggg ggacttacga
    49321 attattttaa aggctatgac agctgtattt ctagattgtg tttgcatcaa gttgcaataa
    49381 aaactagaaa tcaaatcggg ctgataatca tttataacta atcgatacat aggaccaaat
    49441 ataggtctat gttcttttat ttcataagta tcgtaaaaca gaattttgct atggattaat
    49501 ccaatcgact gatatgttta gtaaataaag aaagaggaat gtcaatgatg aaaaaagaaa
    49561 tcgtattaac agcagcaatg atgttattca gcatggtagc aagtacgact tttgtttctg
    49621 caactgaagt atacccaaag gaatataaca cagaaggaac gattacattt gaagcaggag
    49681 atgagggtgt aactcctcct gttgatccag agaatcctga tcccaataaa cctgtcgatc
    49741 catcagatcc tccaagccct ggaacaggag gggcattatc gattgattat ggatcaaaat
    49801 tcaaatttgg gactcaaaaa atttctactg cggataaaac gtattatgca gctgctgatg
    49861 tgatgaacga tgggtctaga aaaccgacat atgtccaagt gacagatagg agaagtacct
    49921 tatctgggtg gaaattaagt gtctctcaac cagaacagtt taaaactgcc agtggggatg
    49981 aactagttgg agcacagctg aaatttacga aagggcaggc tgtttctttg gttgatccaa
    50041 catatacgcc gcaaacggtg aattctgaat taacgttgac accgggagga aacaatacgt
    50101 tagcaattaa tgctaaatca ggaacaggtg tggggacttg ggtctatcga tttggtgcaa
    50161 atgaaaatga aaatcaagat gcggtacagc tttcggtacc cggtaaatct gtcaagttag
    50221 cgcaacaata ttccactaaa ttagtttgga cattagaaga tacacctaac aactagttta
    50281 ctgaaaagaa ctgttaaaaa taaatacgta gtcatattgt cttgcgaggt aatcgagcaa
    50341 aaattgtcaa aggctaggct aaatttgttt ggatacgaat agttcatcta ggaatttaac
    50401 gatcaggagg aaaagatata ttgaaaaaga ggaggatcgt gctcatttta agcagtctct
    50461 tagtgactaa tatctttccg atcatagggt actctttgga attgaatcag ttggagcatg
    50521 gaaatagaag agacgaagaa agcaaactca cttcgacatt cgacagttta atagaatcca
    50581 accagacaca agaaagcagt gaggatttaa gtatttcgag tagtggagag accactgaca
    50641 acgtagaagc tgagggttcg gacaaacaag gactagaaga tgcaccaatt tctgcggttg
    50701 agctgcctga cgatcccaat attatttcta tcgataaaat ttttcaaaca cctatcggta
    50761 ctagcacgtc catattggaa aatgggaagt tactgcagtt gaacccagca gaaaggtctc
    50821 aaagaggagc tatttggtca aataagaaga ttagtttact ctcggatttt acatttaaat
    50881 cttatttata cttaggaaat caacgagata gagcgggaga tggcatgacc tttacattga
    50941 ctggcgatcc tagaatggct agtaatccca gtcaagttat tggatcgcca ggtatgggaa
    51001 ttggctcata ttctactcgt tctggccaac cttacattgc gaatgcgctc tcgattgagt
    51061 ttgatacgta taaaaatagt ggttcttctg atcgaatgga tcgagagata actgctgata
    51121 atagagggca tggacatgtc gcttttgtca cgcctaaacc aaataataat aattattctg
    51181 gagagcatac tggcgtgacg gtagctccta catttctttc taatggcaca tggcgcttgt
    51241 taataattaa atgggacgcg gagcaaagaa gtcttactta tgatttggaa ggtgtcggtc
    51301 gctcttctcg gattatacct gatctcatta ggcagtttgg ttctacagaa gtaaattggg
    51361 ggtttacttc ttcaacaggt actttttttc aagaaaatgc tgtcgctgtc acgcgacttc
    51421 catcgaatgt cacatcaaca gctgatgtaa aagtcaacga tagtgattat ggggccatcg
    51481 ctgaagctgg aggaaacgat aagataacta tcagaaatac attgaaggtc gagggggatt
    51541 ttcttgaata taagaaaatg cagactagta tcgacctccc acaagaatta actattcccg
    51601 agcatatctt gatcaatgga aatcaaattg atgccaaaga tatcttgata accggaaatc
    51661 gagtcacgct aaatctgaca gattatttta ttgctggaga tacgttaata atcgaattgg
    51721 agacaacttt gcaggaagta attcctgaaa aagtactcac tatgcatttt gaataccttg
    51781 aaaacgggaa tttgattgag caatcgaatg aaataaacct aaagatcgca aagctgaagg
    51841 agatagatat ccatgtttat tataaagata aagacagtca gattgatatt gctgaaatga
    51901 aaacaatcac tgggaaaata ggagagaagt ataaagaaac tcctattgat attgaagggt
    51961 atgtttttga ctcggattca gcgaatacag aaggcatctt ctccgaaaat acagaggata
    52021 tctattttta ttacagaaaa ggcaaacttt atctctcaac tgctcctcaa tatgtcaatt
    52081 ttggaaaaca taaaatatcc agtagaccat taagtgaatt cggacagttt tcaggtgggt
    52141 tgaaaattat agatgaacgg gcttttggaa gttggaaatt acaattgaaa caagagaaag
    52201 ttcttacaaa tggagatttt gagcttccag aagctctttc ctttgttacc tctgaaaata
    52261 caacggttat agggacatcc gctgttacga ttttcgaaag tgatcaaaag ggtgaaagcg
    52321 atttgtcctc tcttttagat tctacagctc atcgtggaat cagaatagat attcctgtag
    52381 aaaaccaaag aatcggaacc tttgagggga aactatcttg gatacttgca gatgtcccag
    52441 gaaattaaca ggaagagaag aatataacaa aattattgag tttcaagaag ggcacaggta
    52501 ccataggctc ttgtgtccca cagagacaag attttgttat agtcttctct tacatatgat
    52561 taaaggagcg attttcttga gaccaaaaca acaattattt ataacactcc tagttatttt
    52621 aggggcactt tttaacaata tatcagtgtc agctagtgca tttaattttt cagtgacgcc
    52681 gataacttca gagaatcaaa tcgacaagag aaaaacttac ttcgatcttc aacttgatcc
    52741 ggatcaggaa gtagaagtaa aagcggaatt gagaaatgat acagaaaaag aagtgaaaat
    52801 cgatatttct gtgaatagcg caaccaccaa ttcaaatgtg atggttgaat acggaaaaaa
    52861 cgagattgaa aaagacatga gcctgatctt tgatctggta gattatgtga gctatccaca
    52921 gatagtgacg ctaaaaccta aaagtgtcca aaccgttgct tttcatgtca ggatgccgaa
    52981 tgagcgattt gatggtgtat tggctggtgg gatcacattc caagaaatcc agacggaaaa
    53041 ggaccaaaca gaaactaagg atcaaagctt atcgattgag aatgaataca cctatattat
    53101 tgctttgttg atgcaacaga cactgaacga agttgctccg aaccttactc ttcatgaagt
    53161 taaaccagat caaatcaatg caagaaatgt catcttagca aacgttcaaa atgatcaaaa
    53221 aacctatatc aatcaagtag ttattgaaac aaaaattaca aaaaaaggac attctgaagt
    53281 tttatatcaa gaggaaaaag aaggattgca gattgcgccg aatacaaatt tttcttttcc
    53341 aactgcttta aatggtcaac cgctaacccc aggtgaatat caccttacaa tgactgtttt
    53401 aggaaatgaa aatgaaaatg gaaaattttc gagaaaaaaa ggaaatacta cgatcaacta
    53461 taccaatcaa tgggtatttg aaaaagattt tacaatagac ggaaaagtag ccaaggaatt
    53521 gaacacgacg gatgttacga ttaaaaaaga caataattgg atttatatct tagtagcttc
    53581 acttttctta ttgtttattt tattatgcat acttttgttt gttttgagaa aaaaagaaga
    53641 gaaagaaaag taaggttaag ataacaaata gtaaattttt attattgtag gatggttggg
    53701 ctctttttat aacaatataa aatataccat tttattaata aaaatgcata ctagtaaatg
    53761 atataattct aataacaggt ttgatcgcga gaaaggccaa ataaaacgct ctccaaaatg
    53821 ttaaaaaaaa gacaggacga tgtatattat acaaacaaga atcgctgaca acagtgactc
    53881 ggatccagga aatcagtttt atccccagct agttggtgaa tgcatggaaa gtaatatacg
    53941 tagaaagagc tgatgactaa tgaattatga gtttttttta agcaaagaag atcacagaag
    54001 ttattcatta cttaaatatt tagaagcaag ctcaaattta tcagagtcaa tatcgaatgt
    54061 tcaagaagag ttgtcgctat cgacatttct cttgaaaaaa acgatcgata agctgaaaca
    54121 tgatttacaa aagttcgatc tagagaaaaa tttgcgagta accgtcagtg aattggatat
    54181 atgtctggaa attaatggaa aatgttctag caaagctctg ttatctcgtt atataacgga
    54241 ttctttatct atgaaaatgg tattggcttt ctttcaggaa aaatatatct cagtagaaaa
    54301 ttttgctaaa cagcatcacg tgagttattc tgtggcttat aaagtattac aaagactaaa
    54361 aagaaatcta aaaaaatatc aaattttttt tgaaaagaga aagttga
//
LOCUS       DACBZN010000010        48646 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.010, whole genome shotgun sequence.
ACCESSION   DACBZN010000010 DACBZN010000000
VERSION     DACBZN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.010"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(23..2095)
                     /locus_tag="IVD64_000592"
     CDS             complement(23..2095)
                     /locus_tag="IVD64_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6147866.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IVD64_000593"
     CDS             complement(2028..2405)
                     /locus_tag="IVD64_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP6147867.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IVD64_000594"
     CDS             complement(2534..3094)
                     /locus_tag="IVD64_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6147868.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IVD64_000595"
     CDS             3244..4536
                     /locus_tag="IVD64_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6147869.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IVD64_000596"
     CDS             complement(4596..5714)
                     /locus_tag="IVD64_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP6147870.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IVD64_000597"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IVD64_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP6147871.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IVD64_000598"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IVD64_000598"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP6147872.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IVD64_000599"
     CDS             8920..9519
                     /locus_tag="IVD64_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP6147873.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IVD64_000600"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IVD64_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP6147874.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IVD64_000601"
     CDS             complement(11214..11558)
                     /locus_tag="IVD64_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP6147875.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IVD64_000602"
     CDS             complement(11695..12831)
                     /locus_tag="IVD64_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6147876.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IVD64_000603"
     CDS             complement(13020..13994)
                     /locus_tag="IVD64_000603"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6147877.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IVD64_000604"
     CDS             complement(14074..14949)
                     /locus_tag="IVD64_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP6147878.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IVD64_000605"
     CDS             15079..16986
                     /locus_tag="IVD64_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP6147879.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IVD64_000606"
     CDS             complement(17527..20805)
                     /locus_tag="IVD64_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP6147880.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IVD64_000607"
     CDS             complement(21076..21621)
                     /locus_tag="IVD64_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6147881.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IVD64_000608"
     CDS             complement(21633..22325)
                     /locus_tag="IVD64_000608"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP6147882.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IVD64_000609"
     CDS             complement(22344..22628)
                     /locus_tag="IVD64_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147883.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IVD64_000610"
     CDS             complement(22641..23204)
                     /locus_tag="IVD64_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6147884.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IVD64_000611"
     CDS             23432..24094
                     /locus_tag="IVD64_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP6147885.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IVD64_000612"
     CDS             24218..25414
                     /locus_tag="IVD64_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP6147886.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IVD64_000613"
     CDS             complement(25515..26891)
                     /locus_tag="IVD64_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6147887.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IVD64_000614"
     CDS             complement(26928..27794)
                     /locus_tag="IVD64_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP6147888.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IVD64_000615"
     CDS             complement(27883..28908)
                     /locus_tag="IVD64_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6147889.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IVD64_000616"
     CDS             complement(28926..29600)
                     /locus_tag="IVD64_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6147890.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IVD64_000617"
     CDS             complement(29784..30677)
                     /locus_tag="IVD64_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6147891.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IVD64_000618"
     CDS             complement(30751..32448)
                     /locus_tag="IVD64_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6147892.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IVD64_000619"
     CDS             complement(32441..33754)
                     /locus_tag="IVD64_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6147893.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37272)
                     /locus_tag="IVD64_000620"
     CDS             complement(33754..37272)
                     /locus_tag="IVD64_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6147894.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37565..38023)
                     /locus_tag="IVD64_000621"
     CDS             complement(37565..38023)
                     /locus_tag="IVD64_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6147895.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38321..38839)
                     /locus_tag="IVD64_000622"
     CDS             complement(38321..38839)
                     /locus_tag="IVD64_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6147896.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(38841..39458)
                     /locus_tag="IVD64_000623"
     CDS             complement(38841..39458)
                     /locus_tag="IVD64_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6147897.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39455..40309)
                     /locus_tag="IVD64_000624"
     CDS             complement(39455..40309)
                     /locus_tag="IVD64_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6147898.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40306..41217)
                     /locus_tag="IVD64_000625"
     CDS             complement(40306..41217)
                     /locus_tag="IVD64_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6147899.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41258..42592)
                     /locus_tag="IVD64_000626"
     CDS             complement(41258..42592)
                     /locus_tag="IVD64_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6147900.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            42800..43630
                     /locus_tag="IVD64_000627"
     CDS             42800..43630
                     /locus_tag="IVD64_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP6147901.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(43874..44123)
                     /locus_tag="IVD64_000628"
                     /pseudo
     CDS             complement(43874..44123)
                     /locus_tag="IVD64_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44314..44508)
                     /locus_tag="IVD64_000629"
     CDS             complement(44314..44508)
                     /locus_tag="IVD64_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147902.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(45013..45198)
                     /locus_tag="IVD64_000630"
     CDS             complement(45013..45198)
                     /locus_tag="IVD64_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147903.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(46048..46389)
                     /locus_tag="IVD64_000631"
     CDS             complement(46048..46389)
                     /locus_tag="IVD64_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6147904.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(46389..46619)
                     /locus_tag="IVD64_000632"
     CDS             complement(46389..46619)
                     /locus_tag="IVD64_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6147905.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            46906..47244
                     /locus_tag="IVD64_000633"
     CDS             46906..47244
                     /locus_tag="IVD64_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147906.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(47449..47688)
                     /locus_tag="IVD64_000634"
     CDS             complement(47449..47688)
                     /locus_tag="IVD64_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP6147907.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(47664..48011)
                     /locus_tag="IVD64_000635"
     CDS             complement(47664..48011)
                     /locus_tag="IVD64_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP6147908.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        14162 a        10370 c         8504 g        15610 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg tcaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta ttattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaaca cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    37561 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    37621 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    37681 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    37741 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    37801 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    37861 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    37921 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    37981 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    38041 aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta
    38101 atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac caattataca
    38161 acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa
    38221 gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa
    38281 atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata
    38341 ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa
    38401 gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa
    38461 ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag
    38521 gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag
    38581 gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac
    38641 tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag
    38701 caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga
    38761 tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt
    38821 tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg
    38881 ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt
    38941 ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg
    39001 cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc
    39061 agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta
    39121 gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg ataatcgatc
    39181 aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaaa agagaagttc
    39241 tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga
    39301 taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac
    39361 aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa
    39421 taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat
    39481 ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga
    39541 caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt
    39601 gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga
    39661 aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca
    39721 atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt
    39781 cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat
    39841 tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga
    39901 ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca
    39961 aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag
    40021 aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat
    40081 agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg
    40141 ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca
    40201 tccaaagcaa tggaatgatc aaaatgattc ctattactgt catgaggaca taacttagta
    40261 ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc
    40321 tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac
    40381 gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc
    40441 cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt
    40501 tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac
    40561 atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac
    40621 gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc
    40681 taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt
    40741 cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt
    40801 gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc
    40861 tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg
    40921 attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac
    40981 gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt
    41041 gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc
    41101 aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga
    41161 gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt
    41221 ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt
    41281 tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa
    41341 tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg
    41401 tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg
    41461 ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac
    41521 ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag
    41581 aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc
    41641 aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg
    41701 gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc
    41761 cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat
    41821 ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag
    41881 tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg
    41941 cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc
    42001 cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat
    42061 cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat
    42121 ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg
    42181 gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg
    42241 cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag
    42301 ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta
    42361 catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc
    42421 gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt
    42481 cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta
    42541 ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc
    42601 tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa
    42661 taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat
    42721 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
    42781 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
    42841 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
    42901 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
    42961 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
    43021 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
    43081 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
    43141 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
    43201 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
    43261 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
    43321 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
    43381 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
    43441 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
    43501 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
    43561 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
    43621 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
    43681 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
    43741 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
    43801 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
    43861 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
    43921 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
    43981 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
    44041 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
    44101 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
    44161 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
    44221 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
    44281 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
    44341 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
    44401 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
    44461 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
    44521 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
    44581 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
    44641 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
    44701 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
    44761 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
    44821 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
    44881 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
    44941 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
    45001 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
    45061 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
    45121 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
    45181 tttagaattt ttactcattc atagacactt ccttaaataa cttttattat agaaggtatt
    45241 gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaacttaca ttatactgga
    45301 atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa
    45361 agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat cataaaaggt
    45421 acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc tgacccaatt
    45481 gcacattttc gatcattaca agtattggtg tcgttagaac aactaaataa aaactagcgg
    45541 tagatttttc agaatctatg ctaatttctg tatggatgtt ccagtgatgg caaatttctc
    45601 ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt aaactataga
    45661 aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc gatatagttt
    45721 aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac tgtcagttgt
    45781 tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta agatatgtcg
    45841 tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat
    45901 tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatgcc atcgaccaca
    45961 ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt tagcatataa
    46021 aaatattata gagtttactt acaatgctta aaacatatac tgtataatct gatcaatttt
    46081 agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag
    46141 agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta
    46201 acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt
    46261 acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa tttcttttcc
    46321 tacggaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat aaattctttc
    46381 ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc gtctttctca
    46441 taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac taacgtaatt
    46501 gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt ctttccgtta
    46561 tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt aacaagcatt
    46621 atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa attccccttt
    46681 gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata aatagaaata
    46741 aattgtagcc agttcatttt ttctttaact aaaaaaataa tccaagaaac attgaaccta
    46801 tggatccaaa agctacccaa atactatagc taaccccccg ctaccttcaa ttttgcagat
    46861 aaaacgcttt gggagaaaat ttcaaagaca acaggccatc ctatcatgcc cgattataaa
    46921 caaaaagctg gctattcctt ttgctctgac aaatttcaat tgggaaagtt cacgaaacta
    46981 gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat agaaacattg
    47041 gttcaacgaa tttcaactag acaagacttt gctattgtag aacgtgagat cgtagctatt
    47101 tttgtagaag aagggatttt acatgatcca actcatatca atgtggataa atggcaacct
    47161 cttatctata aattcagaga atatacaact gccaaccagt cgttaacttt gaattttaga
    47221 ttccaagaat tcaaagagtc ataatgataa acatcgttag gtataaataa aaaagactcg
    47281 cagcacgagt aaaaaacaaa tagaagctat acattagaaa ggtcgactgt gaccctatag
    47341 atgcagtata tgcagcttat gagcatttct tctgttctct ttgtttttgg gatctgtgct
    47401 gaagtctttt tcgttttttg ccgctctttc tttgaaaaat cgattatgtt ataccttctc
    47461 cattactaat tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa tatctgttac
    47521 ccaaggttcg atttgttctt cagtcaagac aagcggcatt cggtcatgga ttggtgcgac
    47581 tgtgcggtta ggttcagtag tcattatgat tgattctgcc attttttcat cctgatggct
    47641 acgataaaaa ccgccaatat acactatttt ttccttattt gaaaacataa atttgtcttt
    47701 ttcttcattc cactcattgg gaaaacacaa cgattttgca aaaatggttg atgaaaggtc
    47761 tttttttctt ctactgtctc tgcacgggca ttaatcatca attgtccttt tttaaatccg
    47821 gtaaatcccc acttcatgat ttccggagtg aggatgtttt cttcctcttt tcttaacgtc
    47881 acaacaagat ttgacggaaa aacttctcca gctgctaatt ctgctgcctg cttcggttgt
    47941 ttcgtctggg cttctcgata ataattctga aggagctctg actctagatc aaaaaaataa
    48001 cgaccacaca tcctaatccc tcccaatctt tttttctatc atacactgca acaagaagtt
    48061 cgcaccaaca aaacactttc aatttttgta gaactaaaaa agttgtgaca agtgacaaac
    48121 tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat attcaacaaa
    48181 catgagaagc tgctttttga aattgttcct tgtgactcgg agtgaaacgg caggcttacg
    48241 acctctgggt aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    48301 aatgtagaaa gctattttca caaaattttt cttaatactc gaaactaagc gactttttca
    48361 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac
    48421 gaaaaatatg agcagctatt tttatttggc tgttcttatt acttgatgca gggcagcttt
    48481 ttcacaacct ctccactaaa cgatgttcaa tcagctaacc ttcggtatta gctcaaaaaa
    48541 cggcaaaata tgaggaacta ttttgccgtt tgtttctcta atactcaggt caaagcgctg
    48601 atttcacaac ctctaataca cggtgttcca gaagtagctt cttcga
//
LOCUS       DACBZN010000011        48208 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.011, whole genome shotgun sequence.
ACCESSION   DACBZN010000011 DACBZN010000000
VERSION     DACBZN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.011"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVD64_000636"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVD64_000636"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP6147909.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVD64_000637"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVD64_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP6147910.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVD64_000638"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVD64_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP6147911.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVD64_000639"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVD64_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP6147912.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVD64_000640"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVD64_000640"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP6147913.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVD64_000641"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVD64_000641"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP6147914.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IVD64_000642"
     CDS             complement(8053..8523)
                     /locus_tag="IVD64_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP6147915.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IVD64_000643"
     CDS             complement(8569..8994)
                     /locus_tag="IVD64_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP6147916.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IVD64_000644"
     CDS             complement(9061..9330)
                     /locus_tag="IVD64_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP6147917.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IVD64_000645"
     CDS             complement(9341..10936)
                     /locus_tag="IVD64_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6147918.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVD64_000646"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVD64_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP6147919.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IVD64_000647"
     CDS             complement(14489..15049)
                     /locus_tag="IVD64_000647"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP6147920.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IVD64_000648"
     CDS             15402..16376
                     /locus_tag="IVD64_000648"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6147921.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IVD64_000649"
     CDS             16546..17946
                     /locus_tag="IVD64_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP6147922.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IVD64_000650"
     CDS             complement(18024..18746)
                     /locus_tag="IVD64_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6147923.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IVD64_000651"
     CDS             complement(18825..19331)
                     /locus_tag="IVD64_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6147924.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IVD64_000652"
     CDS             19454..21253
                     /locus_tag="IVD64_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP6147925.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IVD64_000653"
     CDS             complement(21321..22895)
                     /locus_tag="IVD64_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147926.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IVD64_000654"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IVD64_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP6147927.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IVD64_000655"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IVD64_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP6147928.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IVD64_000656"
     CDS             complement(24672..25310)
                     /locus_tag="IVD64_000656"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP6147929.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IVD64_000657"
     CDS             complement(25311..25961)
                     /locus_tag="IVD64_000657"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6147930.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IVD64_000658"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IVD64_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP6147931.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IVD64_000659"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IVD64_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP6147932.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IVD64_000660"
     CDS             complement(29104..29844)
                     /locus_tag="IVD64_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP6147933.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IVD64_000661"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IVD64_000661"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP6147934.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IVD64_000662"
     CDS             complement(31215..32153)
                     /locus_tag="IVD64_000662"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP6147935.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IVD64_000663"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IVD64_000663"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6147936.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IVD64_000664"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IVD64_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP6147937.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IVD64_000665"
     CDS             complement(35313..35618)
                     /locus_tag="IVD64_000665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6147938.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IVD64_000666"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IVD64_000666"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP6147939.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IVD64_000667"
     CDS             complement(36724..37008)
                     /locus_tag="IVD64_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147940.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IVD64_000668"
     CDS             complement(37026..37916)
                     /locus_tag="IVD64_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP6147941.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IVD64_000669"
     CDS             complement(37936..38154)
                     /locus_tag="IVD64_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147942.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IVD64_000670"
     CDS             complement(38124..39824)
                     /locus_tag="IVD64_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP6147943.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40090..40863
                     /locus_tag="IVD64_000671"
     CDS             40090..40863
                     /locus_tag="IVD64_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147944.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="IVD64_000672"
     CDS             complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="IVD64_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP6147945.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41579..41681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41687..42658)
                     /locus_tag="IVD64_000673"
     CDS             complement(41687..42658)
                     /locus_tag="IVD64_000673"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6147946.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="IVD64_000674"
     CDS             complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="IVD64_000674"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP6147947.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44628..45446)
                     /gene="purR"
                     /locus_tag="IVD64_000675"
     CDS             complement(44628..45446)
                     /gene="purR"
                     /locus_tag="IVD64_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP6147948.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45613..46437)
                     /locus_tag="IVD64_000676"
     CDS             complement(45613..46437)
                     /locus_tag="IVD64_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6147949.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46410..47090)
                     /locus_tag="IVD64_000677"
     CDS             complement(46410..47090)
                     /locus_tag="IVD64_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6147950.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47113..48051)
                     /locus_tag="IVD64_000678"
     CDS             complement(47113..48051)
                     /locus_tag="IVD64_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP6147951.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
BASE COUNT        13201 a        10455 c         8695 g        15857 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcaactcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttagtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctga ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcgcgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgt gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacatccttt aaataaaaaa tacaaaaatc acaaaaatta
    39961 attgaaaatg agagtgtttt caatgaaaac gtgattttgt aatggaattt taaaataaaa
    40021 gaaacaagct tttttaatgc aaatgttgta aaataaaaga gattaagagg aaataagagg
    40081 aggaacatga tgactgagtc agtttatgta catattgata ccacaagtaa tgctgtttta
    40141 acaaaagggc tgactgctac tgactttgca aacagtatcg tgcattttcc tcaaaacttg
    40201 ctcttgcttg atccatctgc ttctgctgga gaatatgaat cccatacagg gctgaaagta
    40261 atccgtggta cagaaaatat tcagcgtttc ttttcctctt ctcgtaacag gcgtggattc
    40321 gatgaattga agtggatcga ctttactgat ttaacgatgc tgaaagaatt gacaccttta
    40381 gaaatttcag aattattata ttttggtcat atgaaaacac atcttcattc gccgtttttt
    40441 tataaactac agaataattt tgtatacttc gatcttaatg atcagctgaa tcggatttat
    40501 tatcgctatt tagatgaatt ttataggatt tttgctagta aattaagttc gatcttgtca
    40561 gataagatca atgtaaaaaa atcttttttc aaaaaagaaa cacctgttga aaaaatcagt
    40621 gtggaattat tgaaagaaat gcgtaatttg atgcaggaag ggatcatttt ttcttttgat
    40681 caagttggtt tgatcgatgg taaatatcgt atcccaatct atgtgattga agattatatt
    40741 tggaaattaa aaggcacccg gtatcgagaa gaggaaatca tcgctacttt aatctactct
    40801 gtcaccgatc gacactggca attggtagaa gatgccgatc tttcttatct gccattgaag
    40861 tagatctatt tcttatcaaa aaataaaaag gaaacaagaa actaggacat gattctgtct
    40921 caagtttctt gcttcttttt tatttacagc tcctgtcgtt tatctggcac aaaaatcgaa
    40981 ggagtcgttt gttggatccc taataaaacg atgatcgtta caatagctgt actcaacccg
    41041 ctggcaccat tcattacgaa agaaaagagc caagaattca tcccccatag tgcatacgct
    41101 ccccagaaaa ccactcctgc aataaaatgc caaaaatatc tggaaagtgc gccaatcaat
    41161 gcaccaaaca cgatgctttt tctaatttgt ttcgtattat cgtctacata gcctttttgg
    41221 acatttgaag ccatgagccc tgctgcgcct gcaaaagtat aggcaatcgg gtactcaatc
    41281 aacacttgaa cgacacttaa ataataaact tgtcccaacg gaaaatgtag gatgccccaa
    41341 atgaaacctg ccagcatccc tgcctttgtc cctcttctta acgcaaaaag agtcaatgga
    41401 atctgcccta atgaaataga aaaactgctg ccgatctgga tcggaatcaa cgacaacacc
    41461 atcgctaacg ctgcaacaat cgttccttct acccaaactc tattttttcc cataaaaaaa
    41521 cttcctcctt tgaataaaaa agtcacaaag gaggaagcag ctttcgaaaa tagtcaaaca
    41581 gctgtttatt cacaattcct acgctcgcat aaccgataca ggtacgaagg gtttagaaat
    41641 agattcaatc tcagcccaat tggactcccc tttgtgagtt tatttattat ttattttttg
    41701 tttcaaacaa tggactaact ggtttgtttt catggatacg tttgattgca tctcccatta
    41761 gtccgccaac acttacttca tcgattttgt cgattttacg atcttctgga agatagatcg
    41821 agtctgtaac aactagacgc tcgatagctg aatcttctat tctttgtaaa gcagggccgg
    41881 ataatactgg atgtgtacat gaagcataca cactagttgc accagcttct ttcaaagcat
    41941 tggcagccaa tgtgatcgta cctgctgtat cgatcatatc atcgatcagt acacaagttt
    42001 tgcctttgac ttgtccaatg atattcatta cttctgcaac atttgctttt ggacggcgtt
    42061 tgtctatgat cgcaatcgga gatttcaaaa attccgctaa tttacgtgcc cgtgtgacac
    42121 caccatggtc tggtgaaaca acgacaacat catcgccttt gattcctttt tctaagaaat
    42181 aattggcaat aagtggcgca cccatcaaat gatcgactgg aatatcaaag aaaccttgga
    42241 tttggactgc atgaaggtcc aaagtcagca ttcttgttgc accagctttt tgaatcatgt
    42301 ttgctactaa tttcgctgtg attggttccc gtgcacgtgc tttacggtct tgacgcgcat
    42361 agccataata aggcatgact acgttgattg tttttgcact tgctcgtttc agtgcgtcaa
    42421 tcatgatcag caactccatc aagttatcat tgactgggct gctcgttgat tgaataacat
    42481 agacatgcga tccacgaata ctttcttcaa tgtttacttg aatttctcca tcactaaatt
    42541 gagtaacaga tgattttcct aactcgacgc cgactgctgc tgcaattttc tctgctaatg
    42601 ggcgattgga atttaacgca aaaattttta atcttgggtc aaagtaatgt tttgacatgg
    42661 ggacctccac tttctttttc acaactctag aattctacct aataaatagt agtcatattt
    42721 tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag ataagggagt ttttttgcat
    42781 atccttcttt attgacttgt cttgctctcg caattgccag cgcatattct ggaatattgt
    42841 ctgtaattgt tgacccggca gcaatcgaac tgtttttagc tacctcgaca ggtccgatga
    42901 tattcgtgct agaaccaata aagctgtggt cgccgacagt cgtatgatgt ttgttctttc
    42961 catcatagtt gacgaagaca acaccacatc cgacattgat ttcttctccc agcgtagcat
    43021 caccaacata agtcaagtga ccgactttag ttcctttgcc gatttgagca tttttcactt
    43081 ccacaaagtt tccgatatgg acgttttcgc caatttcagc ttttggtcgt aaatgtgcat
    43141 aaggtcccac atctgcatgt ttcttgacat ggctgctttc aatgactgag ttttcaatga
    43201 cgacgtgatc ctcaatccta ctgtctacga tttttgaatg tgacccgatg acacaatcag
    43261 aaccaatgac tgtatttcct tggatttgta cacctgcttc gatcaatgtg tctggaccga
    43321 tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac aaagcttacg ccattgacca
    43381 tatgcatctg gttaattcgc tttctcatga tctcatttgc tttagctaac gcgatccgat
    43441 cattgactcc cattgattct tcaaaatcat ccgtttgata agcagcaact gtatggcctt
    43501 cttcttttaa gatttcgatg atatctgtta gatagtattc accttgagca ttgtttgttc
    43561 caactttgct taacgcatcg aacaaagctt cgttgtcaaa acagtaagtt cctgtattga
    43621 tttcttgtac aagagcttct tctggtgttg catctttttg ttccacgatt ttttctacga
    43681 tcccaatatg gtcacggatg attcgtccat atcctgttgg gttttctgct tgtgccgtca
    43741 agatcgttgc acttgcattt tttccttggt gatattcaaa aagattgttc agcgtttcag
    43801 tagtcaataa tggtgtatcg ccactgataa ctaacgttgt tccttttttt ccttccaaga
    43861 aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg ttctgcttgt aacgcatatt
    43921 tactgcgttc tcctagttgt gctttaactt gttctgctcc gtgtccaacg atcgtaatca
    43981 cttcatctgg tttcgtttca ctgacccgat taatgatatg ttctaccata ggtttaccag
    44041 aaacgggatg taaaacttta tataattttg acttcatgcg ggtacctttc cctgccgcca
    44101 aaataattgc ataacgtgcg tccaactgcg tcactcctaa atatagtttg ctgatttcag
    44161 tttatcccag tatacccatt ttttcaacaa aacaagtgat tttcttttat cccagcgttt
    44221 tctggataaa aaaatagata gaccataacg ggcctatcta tttggtaacc ggtgctctcc
    44281 tgccaactcc tcggtcacaa gtcacactac tcataaaaca tgaaaagctg ttttttgaaa
    44341 tcgtttcttg tgacttagag ttaaacggta ggcttacaac ctcttcatta aacgtttttc
    44401 aatcagctga ccttcggtat tagctcaata aacggcaaaa tatgaggagc aatttttccg
    44461 tttattgagc taatactcag gacaggacgc tgatttcaca acctctagat aaacggtgtt
    44521 caaaagccag tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa gccattttca
    44581 caaaattttt cttactactc gaaactaagc ggctttttca caacctctta ttctgaaaaa
    44641 taatttcctg gcacaaccgt aatatttttc gtttgagtat ctacatcttt gacataaagc
    44701 agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc aaatacagtg
    44761 atgccaacaa gttctgattc gaattcttca atcaagctct tcattccgtt gactgttccg
    44821 ccgcctttca tgaaatcatc cactacaagg acacgtgaac cacgtttcaa acttctctta
    44881 gacagttcca tcttttcaat tctttcagaa gatccagaca catagttgac acttactgtt
    44941 gatccttctg ttatttttga gtcccggcgt acaataacga atggcgcatt cagataatag
    45001 gacacagctt gggcaatagg tacgcctttt gtggctacag tcattacagc atcgattttt
    45061 tcaccaaggt atttagaagc aatgattctt ccaacttgac gcagcagttc aggttctcct
    45121 aacaaatcag agagataaac atatcctcct ggaagcagac ggtcttgttc tgatagacgt
    45181 tctgctagag attcaatata ttttttcgcc tcttcatatg aaatttcagg gatgaacaat
    45241 acaccacctg cagcacccgg gattgtttcc aaggttccat accctcgttc tttaaatgtt
    45301 ttcttgatga ttcccaaatc ttcactgata gaagatttag cagattcata acgttcggca
    45361 aagcttgtta aggaaatcaa ttcatgcggg tgattcaata aatactgtgt catgtcaact
    45421 agacgttcac tgcgacgtac tttcacctat tccaactcct tttaagaaat tttatcgttt
    45481 tacattatat aagtttttct tcaaaaattc gagcatttta tgaaaaaata agaaaatagt
    45541 tcggtattta ttaaaataaa aagaagccta taaaaaattt ttcgttttta taaggcttct
    45601 tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt
    45661 tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag
    45721 ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc
    45781 aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat
    45841 tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat
    45901 cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc
    45961 atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac
    46021 gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag
    46081 ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag
    46141 cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt
    46201 tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg
    46261 attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc
    46321 agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc
    46381 aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga
    46441 gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga
    46501 tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga
    46561 taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag
    46621 tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat
    46681 tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca
    46741 tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa
    46801 cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga
    46861 atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct
    46921 tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt
    46981 tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca
    47041 ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct
    47101 ccattctaaa acttaattga tactttcttt taaagctttc agattttttt ccatgacaga
    47161 aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac
    47221 ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc
    47281 ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg
    47341 acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta
    47401 ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc
    47461 ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc
    47521 attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt
    47581 tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga
    47641 atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc
    47701 gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga
    47761 atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc
    47821 tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag
    47881 tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt
    47941 cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc
    48001 aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct
    48061 cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata
    48121 aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta
    48181 aatcgtaaaa tggatgagtt gctgctca
//
LOCUS       DACBZN010000012        41266 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.012, whole genome shotgun sequence.
ACCESSION   DACBZN010000012 DACBZN010000000
VERSION     DACBZN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.012"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            214..498
                     /locus_tag="IVD64_000679"
     CDS             214..498
                     /locus_tag="IVD64_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP6147952.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IVD64_000680"
     CDS             complement(779..1198)
                     /locus_tag="IVD64_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP6147953.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IVD64_000681"
     CDS             complement(1200..1784)
                     /locus_tag="IVD64_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP6147954.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IVD64_000682"
     CDS             complement(1831..3306)
                     /locus_tag="IVD64_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP6147955.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IVD64_000683"
     CDS             3487..3798
                     /locus_tag="IVD64_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147956.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IVD64_000684"
     CDS             complement(3913..6249)
                     /locus_tag="IVD64_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6147957.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IVD64_000685"
     CDS             6391..7059
                     /locus_tag="IVD64_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6147958.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IVD64_000686"
     CDS             7062..8105
                     /locus_tag="IVD64_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6147959.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IVD64_000687"
     CDS             complement(8342..9223)
                     /locus_tag="IVD64_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6147960.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IVD64_000688"
     CDS             9382..10110
                     /locus_tag="IVD64_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP6147961.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IVD64_000689"
     CDS             10266..10583
                     /locus_tag="IVD64_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6147962.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IVD64_000690"
     CDS             10576..11184
                     /locus_tag="IVD64_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP6147963.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IVD64_000691"
     CDS             11200..12030
                     /locus_tag="IVD64_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP6147964.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12656
                     /locus_tag="IVD64_000692"
     CDS             12168..12656
                     /locus_tag="IVD64_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP6147965.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="IVD64_000693"
     CDS             12707..13579
                     /locus_tag="IVD64_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP6147966.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="IVD64_000694"
     CDS             13755..14117
                     /locus_tag="IVD64_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6147967.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="IVD64_000695"
     CDS             14126..14878
                     /locus_tag="IVD64_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147968.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="IVD64_000696"
     CDS             14892..16694
                     /locus_tag="IVD64_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6147969.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="IVD64_000697"
     CDS             16971..18011
                     /locus_tag="IVD64_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6147970.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="IVD64_000698"
     CDS             complement(18063..20207)
                     /locus_tag="IVD64_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6147971.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="IVD64_000699"
     CDS             complement(20564..21937)
                     /locus_tag="IVD64_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6147972.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IVD64_000700"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IVD64_000700"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP6147973.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IVD64_000701"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IVD64_000701"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP6147974.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="IVD64_000702"
     CDS             complement(23385..24398)
                     /locus_tag="IVD64_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6147975.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="IVD64_000703"
     CDS             complement(24479..25078)
                     /locus_tag="IVD64_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6147976.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="IVD64_000704"
     CDS             25264..25965
                     /locus_tag="IVD64_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6147977.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="IVD64_000705"
     CDS             25975..28686
                     /locus_tag="IVD64_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6147978.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="IVD64_000706"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="IVD64_000706"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP6147979.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="IVD64_000707"
     CDS             complement(30825..32006)
                     /locus_tag="IVD64_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6147980.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="IVD64_000708"
     CDS             32258..33781
                     /locus_tag="IVD64_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6147981.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="IVD64_000709"
     CDS             complement(33850..34578)
                     /locus_tag="IVD64_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6147982.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="IVD64_000710"
     CDS             complement(34591..35871)
                     /locus_tag="IVD64_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6147983.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="IVD64_000711"
     CDS             complement(36012..36614)
                     /locus_tag="IVD64_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP6147984.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="IVD64_000712"
     CDS             36859..37065
                     /locus_tag="IVD64_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147985.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="IVD64_000713"
     CDS             37377..38096
                     /locus_tag="IVD64_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6147986.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="IVD64_000714"
     CDS             complement(38177..38860)
                     /locus_tag="IVD64_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP6147987.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="IVD64_000715"
     CDS             39078..41264
                     /locus_tag="IVD64_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6147988.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13384 a         7322 c         8275 g        12285 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccatat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcatg
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagttg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctggtttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatgc tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggaa cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatatc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgata
//
LOCUS       DACBZN010000013        40724 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.013, whole genome shotgun sequence.
ACCESSION   DACBZN010000013 DACBZN010000000
VERSION     DACBZN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.013"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(129..800)
                     /locus_tag="IVD64_000716"
     CDS             complement(129..800)
                     /locus_tag="IVD64_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147989.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(793..1287)
                     /locus_tag="IVD64_000717"
     CDS             complement(793..1287)
                     /locus_tag="IVD64_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6147990.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1307..2527)
                     /locus_tag="IVD64_000718"
     CDS             complement(1307..2527)
                     /locus_tag="IVD64_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6147991.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2674..4509
                     /gene="lepA"
                     /locus_tag="IVD64_000719"
     CDS             2674..4509
                     /gene="lepA"
                     /locus_tag="IVD64_000719"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP6147992.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            4706..5617
                     /locus_tag="IVD64_000720"
     CDS             4706..5617
                     /locus_tag="IVD64_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6147993.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            6615..6866
                     /locus_tag="IVD64_000721"
     CDS             6615..6866
                     /locus_tag="IVD64_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147994.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            6949..7221
                     /locus_tag="IVD64_000722"
     CDS             6949..7221
                     /locus_tag="IVD64_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147995.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            7311..7550
                     /locus_tag="IVD64_000723"
     CDS             7311..7550
                     /locus_tag="IVD64_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147996.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            7697..11992
                     /locus_tag="IVD64_000724"
     CDS             7697..11992
                     /locus_tag="IVD64_000724"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP6147997.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
     gene            12708..12953
                     /locus_tag="IVD64_000725"
     CDS             12708..12953
                     /locus_tag="IVD64_000725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147998.1"
                     /translation="MEEIFFCMMVEFLFVSIGFLIMGEAIMIYLFPFDSKDECIFSSI
                     IIFFILACVWILAREIYHQFVLEDGKGIITWFLQKRK"
     gene            12970..13164
                     /locus_tag="IVD64_000726"
     CDS             12970..13164
                     /locus_tag="IVD64_000726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6147999.1"
                     /translation="MEKKNINICFLSGKECTYEECTDISVHERDGMLIVDMSPSDEPF
                     VVKHYFPLCNIESFRIDCEF"
     gene            13181..13546
                     /locus_tag="IVD64_000727"
     CDS             13181..13546
                     /locus_tag="IVD64_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148000.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            13548..14000
                     /locus_tag="IVD64_000728"
     CDS             13548..14000
                     /locus_tag="IVD64_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148001.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            14066..14422
                     /locus_tag="IVD64_000729"
     CDS             14066..14422
                     /locus_tag="IVD64_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148002.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            14612..15910
                     /locus_tag="IVD64_000730"
     CDS             14612..15910
                     /locus_tag="IVD64_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP6148003.1"
                     /translation="MYLIPGISIVMYLLLVSGLILGLMRWSRLKEGYFASVYWRQLLA
                     RMLIDNRFVYTKKQVGEKQTKEKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGS
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     LLISGAIGGGKTYLLYSLIKACLDVGTVDICDGKAADLAALGDIDVFKGHVFYKTNEE
                     MIICLRNALQEMNQRYTYMKTMKEPKYEPGKNYAYYGIPPHFIFFDEWAAFYGSLDME
                     TRNKVDKLIQPLVLKGRQAGMYVILAMQRPDAEYFPSGVRDSLTFRISVGRLSPIGYL
                     MTFGDDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIEEFKKFDTMIVQD
                     FSVLKEEAFTSYEEQIDRQAQSLKGNTKTNGVDEESYTLL"
     gene            15932..16246
                     /locus_tag="IVD64_000731"
     CDS             15932..16246
                     /locus_tag="IVD64_000731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148004.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            16609..17865
                     /locus_tag="IVD64_000732"
     CDS             16609..17865
                     /locus_tag="IVD64_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6148005.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            17862..18407
                     /locus_tag="IVD64_000733"
     CDS             17862..18407
                     /locus_tag="IVD64_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148006.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            18408..18908
                     /locus_tag="IVD64_000734"
     CDS             18408..18908
                     /locus_tag="IVD64_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148007.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            18988..19545
                     /locus_tag="IVD64_000735"
     CDS             18988..19545
                     /locus_tag="IVD64_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148008.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            19565..19909
                     /locus_tag="IVD64_000736"
     CDS             19565..19909
                     /locus_tag="IVD64_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148009.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            19928..20839
                     /locus_tag="IVD64_000737"
     CDS             19928..20839
                     /locus_tag="IVD64_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6148010.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            20918..21910
                     /locus_tag="IVD64_000738"
     CDS             20918..21910
                     /locus_tag="IVD64_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6148011.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            21907..22125
                     /locus_tag="IVD64_000739"
     CDS             21907..22125
                     /locus_tag="IVD64_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148012.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            22138..22548
                     /locus_tag="IVD64_000740"
     CDS             22138..22548
                     /locus_tag="IVD64_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148013.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            22602..25130
                     /locus_tag="IVD64_000741"
     CDS             22602..25130
                     /locus_tag="IVD64_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP6148014.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEYKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            25146..27356
                     /locus_tag="IVD64_000742"
     CDS             25146..27356
                     /locus_tag="IVD64_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148015.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            27364..28455
                     /locus_tag="IVD64_000743"
     CDS             27364..28455
                     /locus_tag="IVD64_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6148016.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            28476..29012
                     /locus_tag="IVD64_000744"
     CDS             28476..29012
                     /locus_tag="IVD64_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148017.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            29234..29464
                     /locus_tag="IVD64_000745"
     CDS             29234..29464
                     /locus_tag="IVD64_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148018.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKVYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            29478..29810
                     /locus_tag="IVD64_000746"
     CDS             29478..29810
                     /locus_tag="IVD64_000746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148019.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            29854..30192
                     /locus_tag="IVD64_000747"
     CDS             29854..30192
                     /locus_tag="IVD64_000747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148020.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            30206..30400
                     /locus_tag="IVD64_000748"
     CDS             30206..30400
                     /locus_tag="IVD64_000748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148021.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
     gene            30901..31308
                     /locus_tag="IVD64_000749"
     CDS             30901..31308
                     /locus_tag="IVD64_000749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148022.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            31492..31847
                     /locus_tag="IVD64_000750"
                     /pseudo
     CDS             31492..31847
                     /locus_tag="IVD64_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32550..33221)
                     /locus_tag="IVD64_000751"
     CDS             complement(32550..33221)
                     /locus_tag="IVD64_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6148023.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQHGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKSI"
     gene            33527..35158
                     /locus_tag="IVD64_000752"
     CDS             33527..35158
                     /locus_tag="IVD64_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6148024.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            35161..36240
                     /gene="uxuA"
                     /locus_tag="IVD64_000753"
     CDS             35161..36240
                     /gene="uxuA"
                     /locus_tag="IVD64_000753"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6148025.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEVPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            36314..37741
                     /gene="uxaC"
                     /locus_tag="IVD64_000754"
     CDS             36314..37741
                     /gene="uxaC"
                     /locus_tag="IVD64_000754"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6148026.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            37834..38481
                     /gene="eda"
                     /locus_tag="IVD64_000755"
     CDS             37834..38481
                     /gene="eda"
                     /locus_tag="IVD64_000755"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6148027.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            38601..39977
                     /locus_tag="IVD64_000756"
     CDS             38601..39977
                     /locus_tag="IVD64_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6148028.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANIQTGDVLEKLYNASTLIPSICFFIVGLILFFLYPLSKDKVLENC
                     ELLKQKYK"
     gene            39987..>40724
                     /locus_tag="IVD64_000757"
     CDS             39987..>40724
                     /locus_tag="IVD64_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6148029.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGEY"
BASE COUNT        13917 a         5970 c         8414 g        12423 t
ORIGIN      
        1 aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa cagtgttcca
       61 gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt ctgtcacaac
      121 ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta acgctcatct
      181 tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt atttgtcagc
      241 aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc atcagaagca
      301 gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca gaactcccaa
      361 aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca aggactggtt
      421 tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag agctgtcagg
      481 accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact gatcaaatgc
      541 atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa aacgattgct
      601 gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat caatgcaatc
      661 cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata
      721 aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc tatttgcacc
      781 tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta cttctgaaag
      841 caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta ttgtatataa
      901 gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg
      961 ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc ctttcgcata
     1021 agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta gtaaagcaat
     1081 cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc gactgtctgc
     1141 ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg aaagctcaga
     1201 agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac ttttagcaaa
     1261 cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa aaataatcgc
     1321 taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt tcttcatctg
     1381 tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc atcaagcaag
     1441 tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag acacggactt
     1501 gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg acaggatctt
     1561 caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga aggaattctt
     1621 ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc tgactatctt
     1681 caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag tagatcatgg
     1741 aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca agatagaaca
     1801 tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct cttggttcat
     1861 ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa cgccaatact
     1921 cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca aatttgtcat
     1981 aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca cttactggga
     2041 tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac atgatttccc
     2101 atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc cattgcttat
     2161 tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca tggttagcat
     2221 attctggata agttccaact ccagtcacac cacccatttt ataacgtgca ccgtgaatat
     2281 tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg aaccatccga
     2341 acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt ttattctcaa
     2401 acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc aattcattga
     2461 attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta aaatcttttt
     2521 gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat tctacaaaaa
     2581 aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt tggtataatg
     2641 gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat gaaagaacgc
     2701 caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa atctacattg
     2761 gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca agatcagctg
     2821 ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa
     2881 ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga caccccaggg
     2941 cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg tgccgtttta
     3001 gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta tttagcgtta
     3061 gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc agcagatccc
     3121 gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga agctgtatta
     3181 gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt ggaatatgtt
     3241 cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga ctctatttat
     3301 gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt caaacccgga
     3361 gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt cggggtcttc
     3421 tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta tatcacagca
     3481 agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc agacaatcct
     3541 gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg tggtctttat
     3601 ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact gcaattgaat
     3661 gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg gttccgctgt
     3721 ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg ggaattcaat
     3781 ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac ggatggtacg
     3841 actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca ggatgtagaa
     3901 gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa
     3961 ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga ttaccgagta
     4021 aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga caaattgaaa
     4081 tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca aaaaagcaag
     4141 ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt
     4201 caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc
     4261 ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat cgttgcccgt
     4321 tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg agatgtttct
     4381 cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc
     4441 tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga gcaagatagt
     4501 gtgaaataac ttataatcaa tcgaaaaacc tattaaatca acctttttga gtagatgcaa
     4561 cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac ggcgatacta
     4621 aaattaacaa aattggaata ttttttcttg aagattttta gctgtcagta tatgatgaaa
     4681 gcatctcata ggaggtgctt ttttcatggt tgataattca agtggaaggg ttcttaaatc
     4741 tctaagaaaa gagaaaaaac tatctcaaaa aaagttagct gatttagcag gtatttcaca
     4801 aagtacttta gttaaatatg aaaaagggtc aagaaaaatc cctaaagacg tagataatac
     4861 tctaagcaag attttgaata tagaaactct aataaaagat gaggaagata agattgagat
     4921 tctcatcggt caactaattg catacagaga tatgaataag ttacttaaca aagagttagc
     4981 tgataatata ggaattagtg aagtattact cagttatgtg ttaaatagaa agagaaatcc
     5041 gtcaaaagaa atgcaaaaaa aaatcgatat ttttctatta agtaatgaaa aagaaatttc
     5101 gaaggagagt aatcgtgata gtgagatatt tagtttatca aaagacgata aaattgttat
     5161 gggaaaaaga atacgagaag tccgaaagaa ccgggaagag acgcttgaaa aatttggtaa
     5221 aaactttacc atctatacag gcaaaaatgt tataagtaga tgggaaaaag gtattaatat
     5281 tccagatatt gaaaaactaa tgaatattgc ctatcttggt aaggtcactg ttccttacct
     5341 tatgtacgga gaagattata agaatatact tccaaaagat gaaagagtta gcgattttaa
     5401 caagataaat tcttttagta tgggattaag gatgaggaaa attaggaaag attattatct
     5461 agaaagggag gagtttggaa agcttttttc tccttcaatt tcaaaatggt ccatagatag
     5521 gtatgagaat ggtagagata tacccaatac taatcgaatt atccaatatg cttatatagg
     5581 aaatctttct ttagaatttt tgatttacgg gatataaaaa tagtttacgt cgatacttat
     5641 taagttatcg acgttttttt attatagaaa aatttataaa aggaggtttc ttgatatgat
     5701 aagaaacaaa aagggtttgt tgaaagttgc tttactggca gcttttttct aagtaacttt
     5761 ttgacaagct caatggcaac acaagcggaa gaagtaatac aagaaataac tgttatgaat
     5821 accagctaga taaaatgggc taggtatgtt atccaacaat ttagtaatca ctagaagcct
     5881 agcgaaagtt cgcttgataa gttatagatg agtattgatt tgtagtcttt ccaaatcaaa
     5941 agggtacaga gccacatgaa atatgttgtc ctctgcagcg tcaggatggg aatccatacc
     6001 atggaaattc ggagatctgt gagggaatac ttacgtggtt aatatgtcgg ttgtgttgga
     6061 cgtagtaatg gtcatagtgg gtagattgac tgaattctta tatgagaaaa tgaaaccaac
     6121 aagaagatag ggttatttat acagaattgg ggatcttttc tagaaaagta atcggacaat
     6181 gcgagattcg tacggtcttt tttaaatttc tcttaaaaaa acggcatcga atgggttggg
     6241 tgattgataa gcaatgaatc atagactgat atagaataaa aaaatgattc tatttatctt
     6301 tttacatcta tcgttttttc aagtaaccgc aaattgtatc agcattcaac aatttttgta
     6361 tacaagttct agtaaaagga tctgaagaaa aagttggttg ttgtgaagat aaaaaaagaa
     6421 tcaggtttta ctgagttttt cttcaaaatt tgtgttatta ggttaggaca aagataagaa
     6481 gaagtatgaa gatttaaaaa aggaatgagg aaaagatagt aatttccgat tattggagtt
     6541 tttttatttt ataaagaaga agcgaacaag ttgtgattcc tagatttcgc gcgtgattaa
     6601 ttaatgagca aaaaatgcgg aaagttttag tgtttaatac acgaggacaa agtttcttag
     6661 gtattgaagg cacacgtgta acggttgatg ggtggtgtga ttgtttcgag gttggaaaac
     6721 atgttgaact tatggcattt actgagctaa aagataagaa caacgtggaa tttgtcgaga
     6781 gtgatattgg ttgggacgat catgaagaag tttatggaaa atttattttg cggaagggaa
     6841 attcttatac gaatggggaa gcataagtag cgatttgtta gagatcattg ataggattga
     6901 aattgtcggg aatatttatg agaatctaga gttatttgag gtgatagaat gagtgatggt
     6961 tttgaacaag tatcaataat taaaaatcaa gttcgggaga ttcttagaaa aaaaggctat
     7021 ttggtagata gttattttga aggagattac gaaacatggg taggtgtcta tgctcgtcca
     7081 gagaataaac ctacctattt agatcctacg acgtcagaag atgcctattt acaaaatagg
     7141 tatcgagtag atggttttaa gcaagacttt gcggagtggt ttgaatggga aattgaaaac
     7201 ggagaagtaa aagaagaata agcgtacttg ttgtatcaaa ataagtcagt agaacaacca
     7261 aaccagtagc tagtaatgac aataatactg gggaatcgga tcattatggt gtgattgata
     7321 tttctattcc agaagatctt aagccagatc ctgcaacaat tggcaacagt ggcatgattt
     7381 ttgattcgtt agatgaagct atgtcatatg gttggaaaga aattaaggat gaaggtagta
     7441 attggtatgg catgatattt ggtgatggtg aagttgccaa taaagctggt attccaattg
     7501 ggaaatggac agttgatttc cacgaagcag gttctttttt tgttctgtaa ttatagtggg
     7561 agggtaaaat cattgatgaa atgtgttcag ataagagcgc cagataaaaa tagaagagac
     7621 agttcacgtc gttatcactt ttggtaacgg tgttttgttt tattatataa aattctcagg
     7681 aaaaggagga attcttgtgt atgcaaaaac gaaaatgaat tggttgaaag cactcgcatt
     7741 ggtggcctta tctcaattct gtttcttggt gggaactgct caagcacatg cagaagaaac
     7801 gacggctaca caacctacgg tggaagccat tgcccaagtc gcttctgaag tgacagaagt
     7861 ccaaccagca gaagccgggg tcccagctgg tgaagccact gttgaacagg cccctgtgac
     7921 agaagcgcca acggttaatt ccaaaacgac tctcgatcag acgattgggg aaacacaaca
     7981 cccagggaat agtcctgtta cttcacaacc agttgaagaa actgcctcaa ttacaacccc
     8041 tgcttcagaa acgtccactc cagaaggaga aaaagctgcc gaagcaacgc cttctgtgaa
     8101 gccagagtct tctgagaaac aagcggaaga aaagcaacga atagaagaaa cagtggaaca
     8161 accagcggct actgctgaca aagaaggaaa aaatgaggaa aacgttgaaa aaacaatctc
     8221 ttcaaccgca gctagtcaaa atcaaccaac gaaactaaac aaagaatcta cggctagtat
     8281 tgaagatcaa aagaaaaaag agcaggacat tccaaaaaca ggtacaccta cactgttagt
     8341 aacagtacca accaatcgtt cggttcgtgt tcgccgtgca accaatacag ctgttttagg
     8401 tggtcggaaa attgattggg gcgaaaatat tggtgttact tctagtccga acaatccaaa
     8461 cttcacttta aattatatgg ttcgttttga gattaatgga caagtggcgt tttgtattga
     8521 gcctggtgtc tcagccgcag aaggtcaagt ttatacagct actgctttgg aaaactattt
     8581 gaaaaattca aatgtacgga aaaaaacaag cttgatctct tatttaggat atgtcacaaa
     8641 taaagacaaa tccgatgaac aatacattgc ggcacaattt atgatttggg aagctttggg
     8701 cacaaaaatc ccaatcaaaa atatccgtgt tgattatgat cgtcgaaaag cagaaattca
     8761 acgtctgctt aataattttg atgtaagagc tacttttcat aatcaaacac atacgataaa
     8821 aattggcgaa acgttaaaag taaaaagtgg caatgacttt ctacaacgtg taaaaggcat
     8881 tcaaaagcca aaagggatca atgtatctat tcatgggaat gaagcatgga tcacagtcac
     8941 aaaagaagca ccagaaaagg caaaaattca tttcaatcag attgaagtat tgggtacgcc
     9001 attagcgtat cgtcgtccag gtgcccaaac aattggtata ttgaatcctt ttgaacctgg
     9061 tgactctgtt gtcaatttga ctattttgaa acagggaaac attcgtgtct ttaaagaaga
     9121 tgcagaaacc ggtgtgaaag cacaaggcgc agcttcttta gaaaatgcgg tgtatggctt
     9181 gtatcaagca gatgggaaaa agatcaaaga aattaccttg aaaaatatga acggcaaagt
     9241 acaggcagaa attaaagatc ttgatcctgg caaatatgtt ttaaaagaaa tcaaagcgcc
     9301 gaaaggctat gcactttctg atgaaacaat tgaaattgtt gttgaaccag gaaaaactgt
     9361 caatgcgacc gcaaaagatc aagtgatcaa aggaaccatt gatttagtga aaattgctaa
     9421 taaagattta gtcgattcaa ccaatccaga taacaaacca aaattagctg gcattgaaat
     9481 ctctttgacg tcaaaaacaa ctggcaaagt ggtgaaaacc gttgtgacag acaatgatgg
     9541 ttacgctact tttggtaaaa atactgttgt ctttgatacc tacattcttt ctgaaaccaa
     9601 aggaaaagaa gggtataaac tatttgaacc acttgaagtc acaatttctg aaaatgggca
     9661 aactttccat tatgttttag aagataaagt aattgaacaa cgtcttaaag tggtgaaagt
     9721 ggatcaagaa acaggaaaag tgattccact tgccaataca gaattcaaga tttttgatac
     9781 tttagccaat cgttatgtca caatggagat tccaaatgat acagaaacaa cggatatctt
     9841 taaaaccaat gaaaaaggct ttttcctaac taacggtaca cttacttatg gaaaagagcg
     9901 ctatcgtttg gaagaaatca aagcacccgt aaattacgtt ttgaatcaaa acccattagt
     9961 gttctcgatt accaatgata cagaaccgat caaagtgatc cattttgcga accgtcaagc
    10021 acggaaaagt gtaaaattgt tcaagtatga agaatggaac aagaagaaaa caccacttgc
    10081 cggtgtgacg tttacgctat ataacggaaa aggtcaagca attggtaaat ataccaccga
    10141 tcaaaatggt gagattttag taaaaggtct ggttgccaat aactattact ttaccgaaaa
    10201 tgtaccatta gaaagtttcc aaccaaatac aggcaaacaa gcattcacta ttacattcag
    10261 acaaaatgcg ccagatgatg gggaaacgtt attggtttct gtacgtaact atttgattcc
    10321 accaaaatta gaaacacaag cgtcaaataa agccgatggt gaaaaagtgg ttgatccatt
    10381 agaaactatc gtcattaaag atagtgtttc ttataccaac ctgtttgtcg gcaaagaata
    10441 cactatttca ggtgtattga tgaacaaggc aacgaatgcg ccaattttac aagaaggcaa
    10501 agaaatcaca agtatcttga cctttattgc agacaaagcc aatggcacaa aagatcttga
    10561 atttgtggtt aatgctagtg ttctgaaagg tcaaacaatc gttgtctttg aaaaactttt
    10621 ccgtgatggc attgaagttg cggtacatgt ggatatcaat gatgacaatc aaagtgttcg
    10681 ggtaaacaat ccaacggttc ataccacagc tgtatttgaa gacgggttga cgatcgcaga
    10741 cccaacgggg ctgttcacct taaatgatac cgttgaaatt aatgagatcc ttccaggaaa
    10801 agcttacttg gtcaaaggaa tcttaatgga taaagcaact ggtgaagcct tacttgttca
    10861 tggtcaaccg gtagaaagtg ctgttggctt tattgccaaa gcagaaaatg aaacggtgat
    10921 cgtaccattt acctttgatt tgacaggttt aaatggtcgt gaaatggtcg tgtttgaaag
    10981 tctttttaga aatgacgaag aactagcaaa ccataaagac atcaacgaca aaggtcaaac
    11041 ggtacgagta acgaacccaa gtgtacgtac aacggctaca cataaaccca ctggcttgaa
    11101 agaggttaat ccattaacga aagtcaccat tacagacgct gtgaaatacc acgatttgat
    11161 cgttggaaaa acatacacag tcaacggtgt tctaatggac aaagtaacgg aaaaaccagt
    11221 gctagtagat ggaaaagaag tctcaagtac cttaacgttt gtggcagaaa caactgatgg
    11281 tacagtcaat ctagacttta tcctagacgc acatgcgttg cgaagcaaag aagtcgtagt
    11341 ttttgaagat ctgtttagag aaggcgtatt attggcttct cacgcagata ttaccgacaa
    11401 agaccaaacc atcaaaatcg tcagtccaac cattacgaca aaagctacca atgcaaatgg
    11461 tggaaaagag atcttagctt taccaaatca aaaggttgtc gaatgggttc aagttaatgg
    11521 gttagcaatc ggtcaaattt acaaactgat tgttcaaggc tacttaacac cagacggcac
    11581 cccatttgaa ggaacacatg cggaaaaagt ctttatggct gataaagaaa tgatggaatt
    11641 cttgtttgaa ttcatcgttg acggtcgcaa ccttgcagga aaaggattga gttttgcgga
    11701 atggctccaa gccaaaacag aagatgaaaa agaaacgttt gaagaagtcg caaaacataa
    11761 cgtcgatcta acaaacaaag atcaaacggt tcaatttgtg gaggcaccaa aaccaccgca
    11821 accagtaaaa ccaaacgaac cacaacctgt aaaagaagtg aaacaagctg caatggtacc
    11881 tatgttgcca aaaacaggct ctgaagacaa tgtattatgg atcatgattg gtatgatctt
    11941 tgtccttgcg gcaggattga ttggttatga tttgtataaa gtcagagact aaagaggacg
    12001 aacaagcggt taggcaacta gccgcttgtt tagtttttag aaagaatttt ttatcaaagg
    12061 caaataaatt attgtgttga gtagtaaatg atcatgagga tacaccaaga aatactgagt
    12121 gtaataaata agaatttaag gatccaaaag agatagaata taattatgtg ataatacagt
    12181 ttcttttttt ctatagaaga aatcacgttt agctactcct aattgctttc ttactttatt
    12241 tagttctgct aggtagcagt atgagaattc aaaatacatt ctttgtactt tgaagaggat
    12301 ttttttcgaa tgtttatttg tgttaattat tttttgtatt tctttggcaa aataaataag
    12361 ttcataaggc aaataaaagt ctatattttt attttcaact gtttggatta gatgttgaat
    12421 tgtttgtttt cgttctagaa tattttttct tgtaattttt tgaaatagat aattttcaat
    12481 tgaagattta aatggaataa acatttcttt tgttagggtt tcagtgcttt tcttttttct
    12541 tgtatagcga tgttttaaat aaaagctaga aatggaccaa acaatagtga cgattgaaac
    12601 tatacttgag atgataatgt gtaattcatt tgaaaatagt tgttgaataa aagtgtttat
    12661 aaaaataatc tcctttttta taaaaagtta gaaaggatgt aaaagagttg gaagaaatat
    12721 tcttttgtat gatggtagag tttttgtttg tatcaattgg tttcttaatt atgggggagg
    12781 caattatgat atatcttttt ccgtttgaca gcaaggatga gtgcatcttt agtagtatta
    12841 ttattttctt tattttagca tgtgtgtgga ttcttgctag ggaaatatat catcagtttg
    12901 tgttggaaga tggaaaagga attattactt ggtttttaca gaaaagaaaa taatggagga
    12961 aaaaagaata tggaaaagaa aaatataaat atttgttttt tgagtggaaa agaatgtacg
    13021 tatgaggagt gtactgatat ttctgtgcat gaacgagacg ggatgttaat agtagatatg
    13081 tctcctagtg atgagccatt tgttgttaaa cattactttc cattgtgtaa tatagagtcg
    13141 tttagaattg attgtgaatt ttaagaggag gaaaaatgat atggcagaaa aagcattgcg
    13201 tatgaagaaa gagtttttag gtcaggtatt tttattgggt tcacgggaac ctaatccacc
    13261 aaaggaaatt aatggtgtgt ctgggcgata tgttcaatta caagcagaat gttcgcgaga
    13321 agaacagcct gctttttttg tttatctcac agctgaagag gataaaagat tacgattacc
    13381 agaaaaagag gtacgtattt catttgaaga agcttatttt gtttttcgta aagaaagagt
    13441 gaatagtcgt gaaacaaatg atatgggaca aacagagcgt cgaacaactg gctatcaaga
    13501 tgtcggtgtc tttttaactg gtgtaaaaat tgaggaggtt ttgtaagatg ggattaaaat
    13561 ttccaaataa ttatgatatt acagaagcat tagatgaaac ttgtatagga gccgtacgct
    13621 ttggcgcatt agaagagtcc gatcgtgtat ttgataatga aacaggcgat tatacgggag
    13681 aaattcgatc acagcgagca caattagttt ttggaaatcc agatgaacca aagattgcac
    13741 gacgagttaa atttccagca gatgtagatt tgactggttt taaacgacgt gatcctgtga
    13801 aattaattga tccagaatat catgtggtat atattccagc acaagatggc aatcgtgaat
    13861 attcagattt agtgatttat gcgaaaggat tagagaaagt agaagctcca acaaataatt
    13921 ctaatccgaa acaatcacaa gaaaaaggtt tagatcaaaa acaaccaagt gctgattcta
    13981 aggaaaaaga gaaaaaataa gttttttaaa gagtaggagg aagtcttcct actctttcta
    14041 tttaaggtag aaggaagggg agattttgaa tctggacaaa accaatcaaa cacttcgaga
    14101 gattatggtt tgttgtcaaa atatccaaca attgattgag tattttggag acaatccaac
    14161 gatttttata caaacaaatg agtatatttg tgcggttatt gggcagtgta tgtttttacg
    14221 tgatcttgtt cgtttttcat taccgtatca gtggaaacaa gaccatcagg aagtggtttg
    14281 gattcgtgta gtacgaatat gtaatatgtg tgcacaccca tataaaaatc gattaaatct
    14341 ttttcgatta tggtttttta ttaaatgtga tattccagta ttagttgctt tttgtcaaaa
    14401 atttttagca caggagaagt aaaaacagaa aggaggaatg tgacaattgg aacctcattt
    14461 ttttaagtat cgtggtcgac gaattcgtta tttcaatcgt tatttatggt tttgttatta
    14521 tggtttcttt ctgttaccat ttttgattct attaagttgg tttagttgga aagtatttta
    14581 tcctcgttta aatacgtatt cgaattggca aatgtatctt attcctggaa tcagtattgt
    14641 gatgtatttg ttgctggtta gtggattaat tttaggtttg atgcgttggt cgcgactaaa
    14701 ggaaggatat tttgcttctg tttattggcg tcagttactt gctcgaatgt taattgacaa
    14761 tcggtttgtt tatacaaaga agcaagttgg tgagaaacaa acgaaggaaa aaatgagatt
    14821 accaaaagtt tatttttatc aaaagaaaca agaattagtg gtttcttttc ctttagatgg
    14881 tggccgtttc cacgaacgat ttttaaaaat gggtagctta ttatccgaag tatttttgcg
    14941 tgatttaatt cgtgaagaac gagaaaaagc ccgaatcaat tatgtatttc tttccgattc
    15001 tggtcggatt cctattgatc aatgtatagc agaaaatgga cgtattcatt taatgaaaac
    15061 attggattgg gtatatgatc aatcgccaaa tctattaatt tctggtgcga ttggtggcgg
    15121 aaaaacctat ttattgtatt cattgatcaa agcttgttta gatgtaggga cagtagatat
    15181 ttgcgatggg aaagcggcag atttagcggc attaggtgat attgatgtat tcaaaggaca
    15241 tgtgttttac aaaacgaatg aagaaatgat tatttgctta cgaaatgcgt tacaagaaat
    15301 gaatcagcga tatacctata tgaaaacgat gaaagagcct aagtatgaac cagggaaaaa
    15361 ttatgcttat tatgggatac cgccgcattt tatctttttt gatgaatggg cagctttcta
    15421 tggttcgttg gatatggaaa cgagaaacaa agttgataaa ttaattcagc cattagtatt
    15481 aaaaggtcgt caagcaggca tgtatgttat tttagcgatg cagcgaccag atgcggaata
    15541 ttttccaagt ggtgttcgag atagtttaac gttccgtatt tctgttggcc gtttatctcc
    15601 cattggctat ttaatgactt ttggagatga ttttaagaat aagccgtttt ataacaaaga
    15661 tcaacctggt cgtgggtatg cttttcctgg ttttgatgtg cctgtagaat tctttgcgcc
    15721 atttgtttac aaagactatc actttattga ggaatttaaa aaatttgaca caatgattgt
    15781 gcaagatttt tctgtactga aagaagaggc gtttactagt tacgaagaac aaattgatcg
    15841 tcaagcgcaa tcgttaaaag gaaatacgaa aacaaatggg gtagatgagg aaagttatac
    15901 acttttataa tttagaagga agtggtgaag tatgggtgaa gttttgtatc gtgtttcttc
    15961 agctgcgggt gaaatttcac ctgattttgc ggttcgaaga ttatacgagt ggattaacaa
    16021 agtagaatac tacacaaaag gttcttatgt tttccgacga attgagcgcg agacattgtt
    16081 tgttacgaga aatcagattg ttttgacaaa agaggatata ttgcgttttc gtcaggtata
    16141 tcggttatgc aaagagaaaa acatacagtt acgtttagcg attttacagt gttttgctcc
    16201 tgaacagtat aaagaattac aagaaaaaga agattcattg gtttagatat cggggtggct
    16261 ttcataggga ctatgggaga acacattgtc cgagtatgtg ctgccgcggc ttgtgtcggc
    16321 gaagccaccg agaccagtcg tggcagcaca tgcttggcat gtgtgcccac aaagaggtcg
    16381 tgcgcttgcg cacggcctct ttgtgtaaag ccccccgata tataatatgg ggggcagaaa
    16441 taacataaca caaataaaaa agtgtattta aatcagttat atcaaggctt tagctaaggt
    16501 tagaaggagt actccttttt agaaaaatta gggggtacca cctgttatat cgggatttct
    16561 agcagattaa gaaaatcgaa atttgttatt ttataaagga ggtagaatat gacacctatt
    16621 gaattgcgaa attatcgaaa aaaattgagt gctattcacg cttcaggtgg agggaaaaaa
    16681 ttactctctc aaaaacgttt tgcacagtta ttgggatgct cggtagatta tataaagaag
    16741 atggaacaag gaaaaaagaa aatcccagag gatttcgaaa aacgaatcaa ggaacgcttt
    16801 cgattgtata tgaaaagcgg aatagatctt cgagcaatga tcgactattt gcgtctctcc
    16861 ttttttggga cagactacaa aatagtgatt caacgtgtgt tacgaatgga tctaacagaa
    16921 tttgaaacac gtccgacggg cttgtatctt tttgattgtc tttctgttcg tggaaatatc
    16981 tgggtattct ggcattcaaa acaaactgta acacaaaatg tacttgtaca gttttcgggt
    17041 caaggttgcc gagaatatga ggaagtcatg cgtgaacaac gaacagattg gcgaacatgg
    17101 ctatattcca tttggaaaaa tcaaggaaag ttggaagctg gttataaacg agtacaatgt
    17161 agtcgattcg atattgcgtt agatgaactt tggggattgg aaacagaaga acattttgat
    17221 ctctttgaat tactagaaaa acaaaaagca ggtttattag aaatggactt taagaaattt
    17281 cagaatattg gcggttgttt tttagatgat ggttatttcc gaagcgaagg gttatccttg
    17341 tattttggat cgcgaaagag tccattgttt ttcaatttct acgaaaaacg ttttgaaatt
    17401 gcgaatcgtg agaaaatttc agaaatagaa gcattaacga aatatggcat ttataatcga
    17461 tatgagttac gcctagctaa tgaaaaagca acacaagcag tagaagcgtt tattttggat
    17521 cattccccta aacgtttagg agagattggc gttggtttaa ttaatgcaaa acttattgtt
    17581 tgggatcgac tggaaagtga tgcacgaatt gttgatgaac gatgggcaaa attgtttcgt
    17641 tcgatggaag aattggattt taccgtaaag ccagaaaaat tctcattaga tcgttcagaa
    17701 cgctggtttt tgaaacaagt agctccgaca ttaaagttat tacaagaagc agatcgtatt
    17761 ttaggaagag actttgtaca agaaggaatt gagaatgctg aactaaatga cgagcaacaa
    17821 agctatctag aatggatggg aacgtcagga gggaaaattg catgaatgca tatacgacaa
    17881 cagaatggct gcgtcttttg gatttaaagg ctgcaattga agcattaaat gaaaagatgg
    17941 ttgatttgtc ttattttcgt tttcgagttc cttatattga acaagcggta aaggctggac
    18001 gttatcagga aaaagaaaac tggcaagaaa ttgcacgctt attagaagtt cgtaaggggt
    18061 acgaacaaga actcgaagag ttggaattta gccgaagaaa aggaacgcta aaattcattc
    18121 gcttttatcg attttcattg ccggtacctg cgattttagc ggtaaaaaaa ggctgcgata
    18181 aaatgaagat atacgaaaac tgtgtggcag cacttagcaa cgaaaaacct cttgtggaag
    18241 aaatctcgtt ggcaactgtt acttgggaaa tgaatcgcca gccaacagaa gaacagacgt
    18301 atctttcttt agaagagatt gagctggaat tagaggaaat cggccgctat gccacttgtt
    18361 ctacctattg tggatcggtg attagtattg ctggtgtgat tgtctaaatg aaaaaaatta
    18421 tgatttatgt tggtgcgtat tggtcgcgtg atccaacagt attagaaaat ccagaagcag
    18481 tacactattg cttgcgccaa ttattctatc tgtataaaga acgattggaa tcattgattc
    18541 gtcagttgcc atatactgac agacgtttgg atgaacttct tctaaggtat ccagcaatgt
    18601 ataaacgacg aaaaaataga ttgttaccaa aagagtatcc cattgaaaaa cgtgaacttg
    18661 aagggcgatt tgtggcttat ttctatgatg atgttcgtat gcgcttagta gaacaacgaa
    18721 tggaaataga aaatgatcgc tactatttta tcaattattg taagaagcgt aagtatcaag
    18781 taactgatga tttttatgat tgtattttac aagatgggga agtgattcta tctaaaattg
    18841 ctccaagttt tcgtgaactt gtgccgatta attttttgaa aaaatgtcat ttgcggattc
    18901 ttccgtaaaa aagagctata aaaggaaaga aaagtagaag ccaaatcggt ttctgctttt
    18961 ttatttttta aggaaggaga agatggtatg tcactggaat ttttaggaga gccaattgaa
    19021 aaggaagagt tcgtttcgca ttatatggat ttgtttattc gggtgataaa aaagctttgt
    19081 acgattgatg aattttatcc acgcgaacaa gactatctag ccgctgaacg aataaaaatc
    19141 agtttattat gggaaaaatt cacagatgaa aataaccgac cgccagatta tcgctattta
    19201 tcagattgtt taacgaataa tgtgctttct aaagaatacc tatttcacaa acgtgagaaa
    19261 aatcaaatgt cggctgaaca tttttgtgat tgttatattg cattattgcg tagtcaacag
    19321 ttattaacgg tagaacgata tggtgcagag tttggttatg tcatgcaagc tgagcgtcat
    19381 agagctacaa gatgctatga gaaagaagat caagtgattg atggctatga agaattatgt
    19441 attcgaaaca atcgctattt gcaacaacgt ttatacgaaa aattaatgat ggatttttcg
    19501 gatctctacc gagggtatga atgttcggtg attcaagaaa ggtagaagaa aagggggaat
    19561 ttttatgtgg cgattattat ttttggtggt atgtggtatg gcattgttct atgtgtatcg
    19621 tgtctatctc ggtccacgaa cggaacgatg gacaagttgg tatagagatc ctttaaaaca
    19681 acgaaaagga ttgaagcggt atcgttatga attacttgtt gaattttcgg atgggcgaca
    19741 agcacagtat tatttttttg caaactatcc aaaatacaac accagtgaac tattgattca
    19801 attattcaaa aataatccat atgttgggtt agaagaaaac ggacgggagt tcttttatcg
    19861 ttcagataat attagtgcgg cgtctatgaa caaaacacga attgcataaa aaattggagg
    19921 aataatcatg aaaaaaacaa cattagcagt cgtaacaaca ctaggaattt cattattttc
    19981 gtttcaatta gggagttcat tggtttatgc agatgaaact gtagaaacac ctaagtcaga
    20041 aattgtagaa acattgccag gggaaggatt atcagatgtt gagacagagc taaatggagg
    20101 aacaacggaa aaaccagcag aacctgaaca tttgcagcca gagaaacctg aaaaagagcc
    20161 agtggcacca gaaccagaga ttcctgaatc aactttgccg actgatccaa ttgaagaaga
    20221 aggtgtgcac agttcaacaa caacggattc aagtatagtc acaactccag aacaatcagg
    20281 aacaaccagt tcttctgaaa cgaataccga ttcgtcaagt acggaacagc caacgatgtc
    20341 gtttaccagt gaagaaccaa aagaagaacc gaaacaacct gctgaaaaat cagaagcaat
    20401 tccaaaagaa aataaaccta ttgttccagc aaaagaagtc acagtatcgg taaccccaaa
    20461 cggtgaaatt acgacgaata attctcaagg aatgagtgta ccgattgtca ctagtaatgt
    20521 agaggaattg acccatattc caacaccaac aactccacta aaagcagaag gtggacaaac
    20581 aattgttggt gtaaaagatg gaattccatt aatccaagat agtgaaggga atttggtaaa
    20641 ggatttgtcg attcctgtaa agaaacttcc aagtggcaat attgaagtca aaacagcaga
    20701 tggaaaaaca aaagtcttac caaagactgg tgaagaaata catgttgtac tttctgttct
    20761 aggaggagtg ttgacaagta ttgctggatt tgttggatat aaaagaaaga gaaaaggaat
    20821 agaaaatgag ggtaattgaa tggactattt ttcagaaaaa caagcttatc gttgtcaatc
    20881 gtacgtacta gtaaaaacat actagaagga gtagcttatg aaaaaacgac aactacggcg
    20941 gaaagaaaaa gtttatacag agaaagtacc gaatcaaagg aaaatccatt caaaaaaagc
    21001 agtgaaatgg atttggtgtt tggttttatt tctttgtttt tcagggggct tggcattttt
    21061 tcaatccatg aaaacgaagg ttcaagtgtc gtttttgaaa gatgaaatcc aacaattaca
    21121 aaagaaagaa gtacaaagtc agtcagaatt aacgtacaca cctgaaattg aatcctttat
    21181 gaatcgtttt gttgctttgt atatgaatac attcgataat catgaacaag aacaacaacg
    21241 tcaagaaata ttaaaaaaag agttttatgc acaaaacttt attcaagaac caggaagtgt
    21301 ttcaggtatt cgaaaattaa ctgctgcttc gtttattgga ttaaaagaaa ttgaaaccat
    21361 tccaacagct agttataaag taacttatga tattcgctcg atggatccag agaataagaa
    21421 agaaacaagt gtgacaatca cacaagtact caatcttccc ttttctattt caaacaaggg
    21481 ctgtcgtatt attgcttatc cttattttac ggcactacca aagaatacag aaaaacaacg
    21541 aatgattact tatgatgaca cattatacga aacggttgat gcgtcggaag cgaaagatgt
    21601 ggaaggattt gtggaagatt tcttagataa gtatgcgaaa aacaatgcaa aagatatgtc
    21661 atacatgatg aaagaacctg aaggactcaa tcagacagct aaagttgatc aagtagagat
    21721 gaaagtattg cgttataaag aaacgaaaaa aacttttatt gtaaaagcaa cagtcgtctt
    21781 tcgagatggt gatacgtcac ttttacaaaa agaacaaatg acgcttgtta ttacgaaaaa
    21841 aacaaatcag tattatgttg aaaaactcac acataattgg ttggatagga aaggagacga
    21901 acggaaatga cactgaaggg aattgtagat tttatattaa cgcaaggtgg ctatttttta
    21961 ataatggtgg ttgtttatct gggattgcga aattttatga aatcaaaagt aggaaatgca
    22021 ttaatatcta tcgcagcagg tgccgcagtc tatttcttct taaatgatcc aacgcgtgta
    22081 tttgatgcgt ttggtaaaat cattgaaaaa ttatttatgg attaggagga tagtgaagtg
    22141 gattgggaac cttacgatta ttcgattgtt tatgcggagc cttatcgcgt acaacagatt
    22201 acaagcaatt ggcgtcttcc atttccagtt gcattagtca atgcagcaac ttttttattg
    22261 gtcgtaatct ttgaatattt atttttacgt tcatttattg gttgggttat ttcttttttt
    22321 ccaccagcag gggttgtttt ttttataggg ctaccctatt tatgtgtgtt agcactggca
    22381 aaactaaaac ctgatggaaa aaaagtgcat ttttatttat gggattggtt ttgttatgta
    22441 gtgcgtatga tgaatggtga ttgtgtgtat tataactggg acaaggtatc aaataatgca
    22501 ttggaagaat tactttttgc gaataagtat gtttatttag aggattagaa aaagggacaa
    22561 tcaactggtt gttccttttt ttaattggga ggtgagatct attggtactt gtaaaagaac
    22621 cagtgaaagc attaaagaaa aattgtttgt taaatggcga ggattgtatc tgggcctact
    22681 atcatgtttt tccacgaacc atttcaaaaa acacaccaaa aaagacacag gaaataaaag
    22741 aaatgatggc tcgtttactg ttacttttag cagattatga agatgtggag ttggtgatgt
    22801 atccacgaac aatgaattta cggacgaaat ttgacacatt aggaaaggga ttttctcccg
    22861 atagtcaagc aattggtgcg tattatgcaa aagaaacagt gacattgttg gaacgagaag
    22921 gtcaagtcta tcgtccttcc tttattttag gaatcaaagt caaagaaaaa gcaaccgtgg
    22981 agagttttta tgatgtacta aatctaatct ggttagatgt agcaggaagt ttagcacgct
    23041 tatttggatt agaagtagat gatttagaaa ctttgttttc tgaaagtgat ccttatgtaa
    23101 aagaagtgac acaattactt tatagctttt ctattcgtcc cttagtaaaa aaagagctgt
    23161 tgtatctcaa tcgtctgcaa tatattcgag gaatggctca taaagtagaa gaacaaaata
    23221 attgggaagt actgaaagac aatcaaggaa cgtatgtacg tatgccgaag gaacataaag
    23281 gccttcttca attagaagat gagttgggga ctagttattt agcgttgtta cctattggag
    23341 aaacacctga aaatatgaga aactgtgatc tatttcaatt tgcacaggat ctttctttcc
    23401 cagcagaatt acgaatgaaa attcactatc cacgcttgaa aggacaggga ggtattggtt
    23461 taaaactaaa ttggctatcg gaacggttca aaacagaata taaggaattg gttcaaaacg
    23521 atgaagaacc tagcgaacgc ttattaactg ttcggggatt agtaaaacat ttaaagcaac
    23581 gagtagataa tggccaacct gtcgtcgatt ggatcgcatg ttttattgta tacggtcaaa
    23641 cggttgaaga agttcgtcaa aatagtgcaa cgattattca acagcttcat acgactatcc
    23701 atatccaccg tgcaaaaaat gatcaacgag aattgttcta taaatgttta ttagggcaac
    23761 cattgggtcg acagttgcat tggaagcacc gaacaactgt agaagcaatt ggcgaatggt
    23821 tgttttcagc agtatcagaa ttaggtatgg aatccggctg gtatattggt cggcaagata
    23881 cattagggga tggtggttct cgaggagaaa aaacaccatt agaagaatta atttatgcgt
    23941 caaatcgttt tgtctttttg aatcttctag ctattgcgga aggaatcaaa ggtgcgttgt
    24001 ttgatgcgcc tcatgtagca gtaactggaa aaaccggtaa agggaagacc tttttagttg
    24061 gattgctctt tttgtatagt tcgtttatga atgtacaaag tttatttgtg gatcccaaag
    24121 gagaaaagcg caaatggttt acgaaactca ttcaagaccc gtattatcag aaacattacc
    24181 ctttgtttgt ggaacatttg caaaaaatga gttatgtcac gttagatgct acaaatcccc
    24241 gtaattatgg cgtattagat ccaattgttt gtttatcccg agtagatgcg aaacaagtag
    24301 cccaggatat gattgatgag ttaagtccgt taggtaccaa tcacctttta aaaggggcgg
    24361 tattaaaggg aatccaagaa gttttagttc aaaaagaagc cggacaacaa gttggcttga
    24421 tgcatgtggt cgagtattta gaacaaatgg aacaaaaaga ggttcgagaa tatggtgagt
    24481 ttttacgctt gacggtagaa gattcgattt tacgtttagg attttcttat ggagaaaacc
    24541 cgggattaga ctttaatgcc aaaacaacga ttttagagat ccaagatttg aaattaccaa
    24601 atgacaatgt ccgaccagaa ctatatacag atgcggatcg aaagtctttg tgtttgatga
    24661 ttagtttagg tcgtttttgt gaaatgtttg gaaaacgaga tagtagtaag aaaacagcga
    24721 tttattttac agaagcatgg gtatttaata attcgaacgc gggacgtagt attattgcag
    24781 caatggcgcg tgtggggcgt tcacagatga atcaattagt gctagataca caatttattg
    24841 gcgacttagg ttcagataaa gaaaaaggga attttggtgt tgtcttcgct tttgatgaag
    24901 acagtgagcg agaaaagatt ttgactcatt tacgtttgga agtaaatgaa gcgaacttat
    24961 ctgaaatgag ccatatgatt aaaggacagt gttggatgtt agatccctat ggacgtgtag
    25021 gaaaactcaa tgtacacagt ttgtttgaag aaatgaccgc tgcattacaa acaacggaaa
    25081 aaacaagtca ctctcaaata gaagaacagg atacgttaat catgcaatag aaacgaggtg
    25141 aaacaatggc ttatataaag cgtaaggaaa agaaatggtg cgtttggctt tttttaacgg
    25201 ttggattcgg actgtttttt gttggtggat tggtatatgc ggtagaaaaa caggtggatc
    25261 aatcgatcta tgagatcggg aaatttcata ttgatacctt ttcttcttat ttatctgata
    25321 gtgaattttc attaggcaaa atgttatttg gagcaaatgg cacagatttt ctaaaaatga
    25381 tgacgaatat gttctttagt ttaagtaaat tgatttggca aggccttgat tttgtcattg
    25441 agaagctcta tgaaggagct gcaatgaacg agttaattca ccagtttttc actttttcgg
    25501 atactattta ttcgaaaata tacgatccta ttggtgcggg gattgtcgtc ttgtatgtgt
    25561 tatatctatt tactcttcgt atatttagag ggaaacatta cgcaaaaatg gtgtttattc
    25621 gatttctctt tgttgtcatt ttttcaatgg tttggtttgg ctatggtagt aacgtaccta
    25681 gtaaaggaga agcttttact aaagggttaa atgatttgtc cgtggaggta gaaggcttta
    25741 ttttttctgc tacaaatagt ctagatgaat tggcgacaaa agatgtcaat ggtgttttag
    25801 tgccaacgac acaaaaggaa gccattcaaa aagtgcgcca actttattat aaagcggctg
    25861 ttgttgatcc gtatctattg ttaaattatg ggacaacaaa tttagaagaa ttgtatcaag
    25921 cagagattga accacaagaa tttctagcca aggaagcgtc aagtgaagaa ttatctgata
    25981 ttgagacaaa agtgaccact gctgcaggag aagatagagg gaaaaagaag gcttatcgtg
    26041 tctatattca accaactaaa gctgtttata aagcagtcgt tggttctatg gtgccattgc
    26101 ttaatctaag tattggatta ccacttgtag tgattggcat gattcgtttt atctttcaaa
    26161 tgttggtgtt gtgtttatta cttgtgattc ctttttcatt actcgcttct tttgttccag
    26221 catttgatta tatgatcatg aataccttaa agtccttttt gagcttgatg tttcaaaaat
    26281 ctttttacag tgtgattatt ttggtggcat ttttgatcta taacatagta gacgatttaa
    26341 ttctaatgaa tactgttttt ggttttttag gaaatatgtt tgtcaaagca ctcctatcaa
    26401 ttttactcat tgtaaaacgg aaagatttat taaagaaact agggttagga gctgcagatc
    26461 aaaccataaa taacacgaaa aacatagcaa gtcattcttt gtctcaaatg aaacaactgc
    26521 ctggtcaact atcaactgct gggcaaaatt tggcaatcaa aggtgcagga ttagcaggga
    26581 atgtgtaccg agcaacgaat ccactcgctt ctgcagcgat acatggattt caaaagtttc
    26641 aacaaaaacg tacaccacaa gaatcggtaa aaggggggca agtgattaat ccctttgaac
    26701 taggtgggcg ttcaaatcaa gacttgcctt atagtggtcg tcatgttcgt tatcatgggg
    26761 tagagcctga aatgtcttct accaaatcaa aaacagagtg gaattctggt ctttcaaatg
    26821 gtttgattcc aaattcgtcc atgaattcag taagaccgaa acaacaggta caatcgttgg
    26881 cacaatcgag taagcaacaa gggaataatc aaagaaaacc attgttaaat aaaggtatcg
    26941 cttttgttgg tcaatcgatt ccgttgcctg ctggaaaagt acccgtgcca caattaacag
    27001 ctgctggttc tctttcacaa cagattcacc aagtgaaaaa tcaggcgaaa aatgggagtt
    27061 atccagcaat gcagcagtta ttgaatcaat actctgttcc taagtcgtcg gtagccactc
    27121 tatcaggaaa tcgttctgtg caacgacaga tacaacgttc gtctgaaagt aaaccgtcga
    27181 tttccgtacc aaaatcctta caacctgcat tttcttcttt taataatcca gtagagcaat
    27241 cgaaaaaaat agaaaagaaa aatgaaacat tgccgatttt ggacgcaaaa ccaggaaccg
    27301 attttacttt accaaaagga cgaacagttc aaaaaccatt atcgaagaaa ggatgaggaa
    27361 gaaatgaaga aagggtgtct aggttgtctg cttttcttta ttcttccctt ttttctattg
    27421 ccattctttt tttcaggaga actttccgag gatggaggag aggctacagg tctagctgtc
    27481 gcttttaatt gggaatatac cgaaggatta ccgatttttt cagagattaa aggacgtggt
    27541 ccgatcacag atgaacatgc gcaagcagca gtcggtgcag cggtaaagta tcagttatta
    27601 ccaagtgtga ttctttctca attaggttat gaatcgactt atggacaatc acaatctgga
    27661 agaaacgata caaacttctt tgggattacc tggtttcctg gttgtcccta tccacaaggt
    27721 tctgcacggg ggattggtgg ttctgaaggt ggcttctata tgaagtttcc gaacctgaaa
    27781 gattgttatg actattacgg ctttttagtg gcgagtcaaa gcaactttaa tcaatgtgtg
    27841 ggcaataaag atcctgggag ttgtttattg attctaggtc gtggaggcta tgcggcggca
    27901 ggtattaccg aaggcagtcc gtattaccaa ggagcgatga gtatcataaa gtcttataac
    27961 ttaacagaat atgatacttt tgcgatcaaa cattggaata gcctaccaac aaaaaaatcg
    28021 tcacttgttg cagggaaaag taaaggtaat gcgaaagttt tagatcggat cattggacga
    28081 tcggtaaata atggagagtg ttatggtggc acggcgtggt atgtatccca actaggtggc
    28141 ccacagttaa tgggttctgg acacatgttt gcgatgttaa taggggaaga ttatgattgg
    28201 acagcctatg gttggcaagt gatcatgaac ccaaaaccaa gtgatttaca agcaggagat
    28261 gtggtgaatt ggcaagcagg tggtgcattg tctcctggaa tctatggtca caccggaatt
    28321 ataacagatg tttcagatgg cggacaacat ttctctagtg tggaacagaa tgccgagcaa
    28381 ggacggatta tggctcggta tcaacgcacg tacaatcaaa caaaaattcg tagtctggtt
    28441 cgtaaggtga aataagaaag gcagtgtcta gaagcgtgaa aaaaggaata attttagtat
    28501 ttagttggtt atgtttgata ggtcttactg gttgtcattg gttttcttct gaaaaacaaa
    28561 aaacacaatt gacaacgaca gaagaaacaa tcgaagcagt aaaagaaaca agtggaactc
    28621 ttcaaagtac tgaaagtaac agtcaaattg gttcattaga agacgagaca gcgcaacaaa
    28681 tagatgaaat gacaaaaaat ttcttaaaag attttgctag aaaatggagt aatttcacag
    28741 atgtttataa acgcaatcaa gctgtgaggg actatatgac ggatcgttgt attaaagaca
    28801 atagtattga tgtagacccg catgtacaaa tgaaaacaac tgggaaactt caccaggtga
    28861 cacaagatat agagaatccg gatcaatatt tgttatttgg agaagaaaca acgaatgagg
    28921 tcactcaatt tgtattactt caattgaaag tagatcaaga agctcaaaag attgatgcga
    28981 tcaagattta ttatgtacga agtgtgtatt aaaataaaaa atgaacactc atatttatta
    29041 tgcaccgaaa aaatagaaaa aaacagactg tttttttaag aagaagtatg tcattcttta
    29101 cttgaaaaaa gttgttatag gacagaaagg ataaaacaaa aacggaaaaa atatgtctta
    29161 tgaataacta caagaactat tctatttttt aggagatgga tacgagatga aaaaattaat
    29221 tggctatagt gtgatgtgta ttggttttgt tggtgtggga ttgtttgtat cgactgaatc
    29281 tgcttctgcg gctgaatggt ataatcctat ctggtataat ccgaacattg aatgtcaaaa
    29341 agtctatccg tatggaccga caaatctgaa aaatggaaaa tgtagagtaa attggaatgg
    29401 cgttgtagat agtgtaatta ctggttcaat ggactctgct ctacatggct ttacgggtca
    29461 ctagaaggag gaagagaatg tctgaagaga aaaaagaaat cgttgaatca ttagtagctt
    29521 taaaagattc tctttctaag attgaatatg ttgataggaa agaaattcaa aaactaattg
    29581 atgatactat tattgaaatc caagatgcgc gttgtgaagg tataaaaatt tctgtagctt
    29641 tatctaaagt aatagaaaaa atgaaccgtt cattagcgtt taatggattg aagctagata
    29701 gacaaactag tctggtatgg gatcatttaa aagacttata tgacaagtct aaaagaagtg
    29761 aacggactgc tgtatcaatc ttaaaaggcc tttgggggat gaattcataa tcaagatatt
    29821 ttaaaaataa cttattttta agtaggaaaa attatgaaaa gaatagttaa aatatttggt
    29881 attttaggtg ttttcttaat aggtttaagt acttttgtta gcgctggaat aagtgtcgat
    29941 gcttctgaaa caaataattt tttagtaaaa caacaagaaa ctgaaataaa tatcattcaa
    30001 aataatttag ccacacataa agattctaaa ggaaatcgat tagtatatat tattgatgag
    30061 agtgatttaa aatcaagttt agatagaatt aattctgaaa ttacagtttc tgaaatccaa
    30121 cagtatctaa atgaatttaa tcgttctatt caaaaaaaat ggccatggat ggctaaccga
    30181 tcagcttcat gaagttgaga attatatgaa agatcaaaaa acaaataata aaactggtga
    30241 acgaagtgcc tgcagttggg ctttcactgg ggctggactt ctacattctg ggatttatac
    30301 agctgctgct ctaggtttaa gccttacggg agtaggtgca gccgttattc catttgctgt
    30361 aggaacagcc tatggaatcg caggagctgc gatctgttaa tatgaaaaaa aacaatatca
    30421 aatagttcat tttttaatta ctttattatt tagcccactt ttttatagaa gtttagataa
    30481 atcagtagtc tttacgatgc tagttatttt acttttaaat agtacgagtt tctatttttt
    30541 gaaaaactat catggtagaa aaaaactttt tcctttattt caatttattt gggatatcag
    30601 tgtgatttta gtttttttct ggttatttaa atagtcactt aagaaaaagg aagaagcaaa
    30661 tttgctatct tcatttgaat gatgttggta ctgtttcgct atgtaggtaa caatacaatt
    30721 ttattatata ccgaaaaaat agactgtttt ttaagaagaa ttatatcgtt ctttacttga
    30781 aaaaagctgt tataagacag aaagaataaa atagaaacgg aaggaacgta aaaacgtctt
    30841 attgaacagc ttaaaaggat aaaaattgat aatcaacaat acttttcagg agggataaaa
    30901 atgagtcgtt tttttaagtt tttttcatta ttaggtgtaa tgttaattgg attaagtacg
    30961 ttagtcggtg ccggaaccag tgtttatgct gctgaaaaag tgaatgaagt tcctgatata
    31021 gaaacacaaa gacaacaggc agtagagagt ttgaaattct atttagaaga agctggttat
    31081 gttgatacag aaacacatca atatatagtt actgatttag aagctataca gaaaaaagct
    31141 gaattagatg gctctgaagg tatagcaggg aaatttattt tagaaaattt tgtgcagcca
    31201 acaggaaagc gtcaaaaacg tgatcttcaa aagtatgctg catgtgttgt tatgaactca
    31261 ttaccatttg gtgtgttgtt tgggatatac ttaatcgtga aaatctaatg caaactttaa
    31321 ttaatgcgtt gcaagggaaa aattatgatt atgctgttaa aatactttta gatgtagcaa
    31381 aaagaagttt aactcctgca caatttgcaa aattcaatgc tgcaactatt gcagctggta
    31441 tagctattaa tgctatttct tgttggggga agtaattagt ggagtgatta tatgagtata
    31501 gatgattgtt tagaagaaca aataagatta tttttaaagt ttttaaaagt taaagttcaa
    31561 aataactact tcacatgtta tagttatgca caccctgttt taaatagtct tccttttaat
    31621 tctttagtgc aaggattgat tacaaatatt gttttattag tctgtacacc aaatgaaata
    31681 tttgtaaaaa agataggcag tggtatttct tcagtaagat taaaagagaa agaatatact
    31741 caaaagatta ttcatattcc taagcctgaa attaagcgtt ttgagataaa agactattct
    31801 gctttaggac tgttctcaat tggttactgg tgataaaaaa agaatactaa aactatacgt
    31861 agaatataag taatcggcat caatcctaat tatgtgattg aatgccgatt tttgttacgc
    31921 gctattttta attatctgta agaagaataa ttttatttta ctcatattcg ttttcacttt
    31981 gaagaagtaa attgcaattt cttataagtt aagcagctat gcaaaaggct atttattcta
    32041 ttatgaaata taagatgtag ttgaaacagg atatatagaa tcaaaatacg aatcttattt
    32101 tgatttttct agttttttga aaaaagcacc ctttgatcgt tgtattagtg gaaaagacaa
    32161 aaaggagcat acttatcatg tcacaaaact acaactaaat atctaaacaa caccaatttg
    32221 atgcctattg taaaaaagta ttgcgaaatg aagccaagtc tattagaaaa tgaaataata
    32281 gaataaaaga aaatgagaaa ccgctgaatg atgtaaatga aggtaagtat ccatgaacta
    32341 attttgatga acaagatatc tattttttat ttggattgga gatactagtt taatatcaaa
    32401 agttatcagc agtcataatt agttatccaa tgtaaaaagt acaaacaata gttgttatgc
    32461 agaatagttt atgaaatata ttacataata agaaaacaag cgatattttt ctgatcagaa
    32521 caatcattta atttaaaaag agttaaaagt tatatacttt tgaaatattt tggatgagta
    32581 gctaaaacaa tattttgctc attaagcatt aaatgaagat gcaacgatgt tagaaagtta
    32641 gcttcttctg gatcttgttt tgttaatgcc tcaaatagtt gataatgttg ttccataatt
    32701 ttttcccact ttaatccttc cgtttttact cgtagccaac gaaatcgttc taagtgtgta
    32761 ttgttatttt ctaaccattg ccatacttct tctcgctctg caatttcaaa acaagcttta
    32821 tggaataaat tatcattatg aaaaaaacta cgagcatttt tttgactaac agctttttct
    32881 tgttctgaaa ttatagtttc taaaatccgt tttttttggt tattaatttt ggcacaacac
    32941 tctaacataa tttgtcgttc caaatgttct ctgacaaatc gagcattttt ggccgaatct
    33001 aaatcaatta atgagatata atttccgctt tgaggaattg tatcaacaag accatgttgt
    33061 cggagttgaa tcagtgcttc acgtattggt gtacgaccaa tagaaagtgt ttcttctaat
    33121 tttttttctg atactttttt tccaggttct aagtcagcat agataatttg tcggcgaata
    33181 gattggtaag cttgattttg gagactagtt ttaattgtca tactcaatgc tccttaactt
    33241 ttattttagc tgatatttta attctgttca tgccatataa tatcaaattt tgaagtaata
    33301 cactagttat aaaaataaaa gaatacatga aaaaaagaaa aaaataagtt tcgataaaat
    33361 cagtccaaaa agaaggagta ttttattttt ctgaataaaa aatacaataa ttctgatatg
    33421 gtagaaaaaa agcaagttta ttccattttt atattgacaa cggttacaac gggttttaaa
    33481 atataaaatg tatcaaaact aacatattag tttaaggagg ttaattatgg tttcgattaa
    33541 aaattggcaa aattctatcg aagaattcaa tgaaatgcag attctactgc cgtgttttga
    33601 tgtcaaagaa gttaagcaag aaggccagca acatccagta tggcttcatt ttggtggagg
    33661 aaatctttat cgaggatttc atgcagaaat tgctcaatgc ttagcagatc aaaatgaatt
    33721 agaaacagga gtagttgtgt gtgaaacatt tgatgaagaa gtagtggaaa aagcttatca
    33781 tgactacaac aatgacattt tggaaattgt tatgagtgga gatggtgaac tcaaaaaaag
    33841 gcttttggca gtaacggctg atagtttata ttgcaatccg aagaatatag cggactatca
    33901 aagagtggtt aaatattttg aagatcctaa attacagttt acaacgttta ctattactga
    33961 aaaaggttac agtttaaaag attctaaagg gcaattcttt tcggtagtca aagctgatat
    34021 caaatctgga cctagcaatc ctcaacatac gatgagtatt gtaacatcct tgttatatag
    34081 cagatttaat gctggagagt tgccgattgc gatggtttct actgataatt tctcacagaa
    34141 tggtcaacgt tttcaagata gtatttttac gattgcagaa gcttggcaaa aagaaggata
    34201 tgtttctgaa aaatttattg cttatttaaa ggataaagaa aaagtgactt ttccttggag
    34261 tatgattgat cgaattacgc caaatccttc agagaatgta aaggaagatc tattgatgat
    34321 ggggctcgag gatttagata tcattcacac aaaaaaacat accaatattg ctgctttcac
    34381 taatactgag gtagttcatt atttagttat tgaagatagt tttccaaatg gccggcctaa
    34441 attggaaaag gctggtgtga ttatgacaga tagagagacg gttgataaag cagacatcat
    34501 gaaggttacg acaagcttga atccacttca tactgctttg gcagtaacag gttgtctgct
    34561 aggatatcaa tcaatttcag cagagatgca ggatgatgat ttggttggtt taatcaaagg
    34621 aataggttat ttagaaggtt taccagtagt tgaaaatcca ggcattattg atccaaaaca
    34681 atttattgat gaagtaatta ataaacgttt gcctaatccc tatattccag atacaccaca
    34741 acgaattgca acagatactt ctcaaaaagt agcgattcgc tatggtgaaa caattaaaaa
    34801 gtatgtcgaa aaagacggaa atgcagaaaa tctaacattt attcctatcg ctattgctgc
    34861 ttggttacgt tatttactgg cagtcgatga tcaaggggaa aattttgtgc caagtcctga
    34921 tccgttattg gtagagttgc aggataaatt atcagtattg aaattaggtg aacaagatcc
    34981 ttcaaatatt cattgtgcgg tacaggaaat tttagaaaat aaagaaattt ttggtttaga
    35041 tttatatcaa gttggggtag gaaagaaaac tgaaaaaatc ttttgcgaaa tgctagtagg
    35101 tcctcaagca gttcgtaaaa caatcaataa atatataaca gaagttggag gaaagtaact
    35161 atggaaatga cttttagatg gtatggggca aatgaagaaa aaatctcact acaagaaatt
    35221 cgtcagatcc cagcaatgaa aggaattgtt ggaacattaa tggatatacc agccggagag
    35281 gtttggccga gagaacgaat taaatcttta aaagatgaag tcgaagccaa tggacttacc
    35341 ttgaaagtta ttgagagtgt taatgttcat gatgatatca aaattggttt acaaacacga
    35401 gatcaataca ttgagaatta taaacagacg attcgcaatt tggcagaatt tgggattgag
    35461 gttatctgtt ataattttat gccagtattt gattgggtaa aatcggattt ggattatcgt
    35521 ttagatgatg ggtcttctac gttagcctat atttcaaaaa atatccctac tgatccggag
    35581 gaaattatta gaaaagtaga aaattctact aataattttg aattgccggg ttgggaacct
    35641 catcggttat cacaaattaa gcaattatta gaagcctata aagaagttga tgaagagaaa
    35701 ttacgagaga actttgcata ttttctcaaa gcaattatcc cgacatgtga agaagttggg
    35761 attaaaatgg cagttcaccc agatgatcca ccatattcca ttttcggatt acctcgtatt
    35821 gttaaaaatc gtgacgattt agattggttg tgtaatgtag tggattctcc ttctaatggt
    35881 attacattat gtactggaag tattgcagaa cgaccagata atgatgtcta tgaaattcta
    35941 tctgaatttt gtcaacggga tcggattcct tttgcacatg ttcgtaatat caaatattta
    36001 agtgacaatg gactcgattt ttacgaagtg ccgcataaat cagaatatgg ttctctagat
    36061 atgtataaga tattaaaaac aatgtacgat aatggattta atggctatat tcgtccagat
    36121 catggtcgga tgatttgggg agaaaccggt agacctggtt atggattata tgaccgagct
    36181 ctaggtgcag tttatttaaa tggtatttgg gaagcgcttg aaaagggggc gagtaaataa
    36241 ttatataatg gagtttattt agaggaaaaa ttttctcttc taaaaactaa cggaaggaaa
    36301 ggagtgttta aatatgtttt tagataagaa ctttttatta gagaatgaaa tagctaaaag
    36361 actttttcat aaatttgcaa aacaccaacc aattatcgac tatcattgtc atttagaccc
    36421 agcatatttg ctagaaaata agcaatacaa gaatttgact caggtatggt taaatgataa
    36481 aggagctgga gatcattata aatggcgatt gatgcgagct aatggtatag aagaagagtt
    36541 gatcagtggt ccaggtgatg attatgaaaa atttttagct tttgtaaaaa cagtggagaa
    36601 ggcgccaggt aatcctattt ttgaatggtc tcatttggaa ttacaacgtt actttgatat
    36661 tgatttgaca atttctgaga agaatgcacc agaaatttgg cgacgtgcta acgaaaaaat
    36721 ccaaacggat tcctatcgcc cgaaagaatt gttggagatg atggatgtga aagccatctg
    36781 tacaacggat gatcctacag atgacttggg gtatcatact gagctttcta aacaggataa
    36841 ttctttcaag gtattaccta ctttccgtcc agataaggtc tggagtatca aatcatcagg
    36901 cttttcgaac tatattttaa aacttagtca agcagcggct ataccaatca aagactttaa
    36961 tgggttgaag caagcattga gccaacgagt ggatcatttt catcaaattg gctgtcgatt
    37021 agcagatcac ggtttgaata cttattttta tgaaatagca gatgaaaaaa tactcgacaa
    37081 tctgttaata aaagctttag caggaaaaac ggatttccat ttatcagaaa ttgcacaatt
    37141 tgttacaggt attcaattac atttgatgtc tgagtataca agatatggtt ggacgatgca
    37201 aatgcattta aatgctttaa gaaataattc aagtatatta aaggaacaaa ttggagtaga
    37261 tgtcggtggt gattcgatgg gggatcaagc tagtttgaca caaaatgttg tagcattgtt
    37321 cgataccgct gaaaagagtg ggtgcttacc aaaaacaatt ttatattcat taaatccaac
    37381 tgattggctt ccactagcaa cagcaatgca aagcttccaa gggggtatga ttcaaaaaat
    37441 ccaattggga tgtgcttggt ggttcaatga tacagctgaa ggaatgcgtc aacagttgac
    37501 tacgatggcg caacaaagtt tgttgtcgaa ttttacaggt atgttgactg attctcgaag
    37561 ttttctaagc tatcctcgcc atgagtattt tcgtcgagta ttatgtcaat tactaagcga
    37621 gtgggttaaa gcaggccgaa ttccagatga tgaaatgtat cttagtcaga tagtggagaa
    37681 tatttgttat cgtaatgcgt ataattattt taattttttt gaaatcggaa gtagaaaata
    37741 atacttgaaa taaaagctta aatttattct ttttaagtta gtacagctag acaaagtatg
    37801 tatccataaa agaaggagat agaggagata gatatgaaaa aagtagaaat tttaagtaag
    37861 ttagaagcgg ctggagtgat tgcggttgta cgtgggaaaa ataaggatga ggccattaaa
    37921 gcaataaatt caattattgc gggtggaatt aaagggattg aactaacttt tacagttcct
    37981 aatgctgaag aaataatcaa agaaattgct aaaaaatatg ctgatcaaaa agatgtaata
    38041 atcggagccg gtacggtttt agatgcgttt actgctcgta tcgcaattat ggctggggca
    38101 gaatatatcg ttagtccatc ttttgatcga caaacagctg agatttgtaa tttatatcaa
    38161 attccttatt taccaggttg tatgacaatt actgagataa aggaggcact tactgctggc
    38221 gtagatatca ttaaactatt cccaggttct gcttttggtc catccattgt aaaagcctat
    38281 caagctccat taccacacgt aaatataatg cctactggcg gagtaagtct agagaactta
    38341 gaagattggt ttgaagtagg agttgttgca gttggtgtag gtgggaattt attggcttct
    38401 gttgaaaaag gtgattttga agaagtaagt cgagtagcta gtaaatatat agataagttt
    38461 tatgagataa agaattatta ggagaaaaaa caaataatac tctgaaatgt aaaaaataaa
    38521 tactgactct ttacgcttca gagtatagta cgtactttca taagaatgaa aacgcttaac
    38581 aaaagaaggg agagaattgt atggaaagaa aatttggtat aagagacaaa ataggatata
    38641 tgtttggaga ttttggaaac gattttactt tcatttttgc tagttctttt ttaatgatct
    38701 tttatacaaa agtattgggg ataagcggag ctgccgttgg aacattattt ttagtagcaa
    38761 gatttatcga tgcagttact gatgtaacta tggggcgtat tgttgataga tcgaaagcaa
    38821 ctaagcaggg taagtttaaa ccgtggattt taagaatgag tggtccagtc gctttagcaa
    38881 gttttttaat gtatcaaact tctatgattt cagcttctat gagcgttaag gtagtataca
    38941 tgtatgttac gtatatatta tgggggagta ttttttacac atctataaat attccttatg
    39001 gatcaatggc atcagctatt acttctatta cagaagaaag aacaatgcta tcgacattcc
    39061 gaaatgttgg agctacttta gcaagtttga tcattggtgt attaacacca ttatttattt
    39121 atacaagaga tgattcaggc gctcaaatag ttcgtggtgg atcaactttt acaattgttg
    39181 cgggaatatt ttcgatatgt gcacttattt gttatttaat atgttataaa ctaactactg
    39241 aacgagtaag aattgaccaa tctcaattca aagtttcatt gttaaaatct ttaaaagggt
    39301 tagttaaaaa tagggcatta ctttctataa ttggtgcggc tattttctta ctccttgctc
    39361 aattattaat tatgagtatg aataattatg tatttcctga ttactataat gatgcacagg
    39421 gaatagtttt aatgaatttt atcaatccta ttttggtttt aatagttgtt tcacctttaa
    39481 ctttgttttt aagtaaaaaa tatggaaaaa aagaactggc atcagtagga atgttctttt
    39541 cagcaatagt atatttgatc ttatttttga taaaaactag taatatgtat gtattcttaa
    39601 ttcttagttc ggttggctat atgggtttgg gtgtgtttaa tactgttatt tgggcaaata
    39661 ttactgatgt tattgatgat caagaagtaa agtctgatca aagagaagat gggaccgtat
    39721 atgcggtata ttcttttgct agaaaaatag gtcaagctct agctggaggc gtcggaggtt
    39781 ggactctttc tattataggc tatgactctt tagcaaatat acaaacggga gatgttttag
    39841 aaaagttata taacgcttca acattaattc catcaatttg tttttttatt gtaggattaa
    39901 ttttattttt cctttatcca ttatctaaag ataaagtctt agaaaattgc gagcttttaa
    39961 aacaaaaata taaataggag agaaatatga ataattggat attttgtatt gattcagatg
    40021 gctgtgttat ggatacgatg acttataaac acaatctctt tttcggacca attgctgcag
    40081 agatttttga tgttcatcat agagaaaaat tcctacagga ttggaataat attaatttat
    40141 attcagagac tcgtggaata aacagatttt taggtttagt taaaacatta gaaaaaaatg
    40201 atgtaacatc cattaatcga ttaaagcact gggtatctct aacaaaagaa ttgtctaatc
    40261 catctttgaa gagggaaata gaatattatc agtcagagga tttattaaaa gctttaaaat
    40321 ggtcggaaga agtcaatcaa cagattagtc aagctaaagg attagataaa ccctttttaa
    40381 gtgctagaga tactataaaa gctctgaaaa aatatgggaa gataattatt gtaagctcag
    40441 ctaataagga agctgttcaa gaagaatggg aaagacatga attattatca ttagttgatg
    40501 aactgtgttg tcaagataaa ggaaaaaaag aagacattat tcgaagtgta attgaaaatg
    40561 gttgtgattt ggataagata ttaatgattg gtgattctcc tggtgatcta gaagctgcaa
    40621 ataaaaataa agttttcttt tatcctattt tggttaataa agaaaaagaa tcatgggaaa
    40681 acttaagaac tgatgtttta tttaaatgtt tatcgggaga gtat
//
LOCUS       DACBZN010000014        38068 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.014, whole genome shotgun sequence.
ACCESSION   DACBZN010000014 DACBZN010000000
VERSION     DACBZN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.014"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(284..1594)
                     /locus_tag="IVD64_000758"
     CDS             complement(284..1594)
                     /locus_tag="IVD64_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6148030.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1606..3024)
                     /locus_tag="IVD64_000759"
     CDS             complement(1606..3024)
                     /locus_tag="IVD64_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6148031.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3264..4832)
                     /locus_tag="IVD64_000760"
     CDS             complement(3264..4832)
                     /locus_tag="IVD64_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP6148032.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4836..5246)
                     /locus_tag="IVD64_000761"
     CDS             complement(4836..5246)
                     /locus_tag="IVD64_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP6148033.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5243..6166)
                     /locus_tag="IVD64_000762"
     CDS             complement(5243..6166)
                     /locus_tag="IVD64_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP6148034.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6163..7041)
                     /locus_tag="IVD64_000763"
     CDS             complement(6163..7041)
                     /locus_tag="IVD64_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6148035.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7028..8365)
                     /locus_tag="IVD64_000764"
     CDS             complement(7028..8365)
                     /locus_tag="IVD64_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6148036.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8368..9471)
                     /locus_tag="IVD64_000765"
     CDS             complement(8368..9471)
                     /locus_tag="IVD64_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6148037.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9545..11053)
                     /locus_tag="IVD64_000766"
     CDS             complement(9545..11053)
                     /locus_tag="IVD64_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6148038.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11019..11711)
                     /locus_tag="IVD64_000767"
     CDS             complement(11019..11711)
                     /locus_tag="IVD64_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6148039.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11733..12707)
                     /locus_tag="IVD64_000768"
     CDS             complement(11733..12707)
                     /locus_tag="IVD64_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148040.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13007..13879
                     /locus_tag="IVD64_000769"
     CDS             13007..13879
                     /locus_tag="IVD64_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6148041.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14337..16535)
                     /locus_tag="IVD64_000770"
     CDS             complement(14337..16535)
                     /locus_tag="IVD64_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6148042.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16767..17759
                     /locus_tag="IVD64_000771"
     CDS             16767..17759
                     /locus_tag="IVD64_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6148043.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17819..19240
                     /locus_tag="IVD64_000772"
     CDS             17819..19240
                     /locus_tag="IVD64_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP6148044.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19233..20732
                     /locus_tag="IVD64_000773"
     CDS             19233..20732
                     /locus_tag="IVD64_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP6148045.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20771..22165
                     /gene="uxaC"
                     /locus_tag="IVD64_000774"
     CDS             20771..22165
                     /gene="uxaC"
                     /locus_tag="IVD64_000774"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6148046.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22324..23007
                     /locus_tag="IVD64_000775"
     CDS             22324..23007
                     /locus_tag="IVD64_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP6148047.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23009..23845
                     /locus_tag="IVD64_000776"
     CDS             23009..23845
                     /locus_tag="IVD64_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6148048.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23856..25610
                     /locus_tag="IVD64_000777"
     CDS             23856..25610
                     /locus_tag="IVD64_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148049.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25886..26227)
                     /locus_tag="IVD64_000778"
     CDS             complement(25886..26227)
                     /locus_tag="IVD64_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6148050.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26295..28466)
                     /locus_tag="IVD64_000779"
     CDS             complement(26295..28466)
                     /locus_tag="IVD64_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6148051.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPVLEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28677..29534
                     /locus_tag="IVD64_000780"
     CDS             28677..29534
                     /locus_tag="IVD64_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6148052.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29605..30462)
                     /locus_tag="IVD64_000781"
     CDS             complement(29605..30462)
                     /locus_tag="IVD64_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6148053.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30447..33149)
                     /locus_tag="IVD64_000782"
     CDS             complement(30447..33149)
                     /locus_tag="IVD64_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6148054.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33162..34451)
                     /locus_tag="IVD64_000783"
     CDS             complement(33162..34451)
                     /locus_tag="IVD64_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP6148055.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34601..35647
                     /locus_tag="IVD64_000784"
     CDS             34601..35647
                     /locus_tag="IVD64_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6148056.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35649..37802
                     /locus_tag="IVD64_000785"
     CDS             35649..37802
                     /locus_tag="IVD64_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6148057.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11240 a         7830 c         6773 g        12225 t
ORIGIN      
        1 tttttcgatt tcgtttttcg aaatctttca gcaactcatt tatcataact tatcgtttgt
       61 tatttgtcaa gaactttttt gaagtttttt tcgatagaat caagcttcgc aagtggttct
      121 ttttaacgac ttagttatct tatcacgtct aaaaacatgt gtcaactctt ttttggaatt
      181 ttcataaaat gtttcaatga agcgcgacaa cgttgtttaa tatatcatta aaaaaaataa
      241 aggtcaagct aaaatattat ttaagcttga ccttattata atttcaacga ttgtcaaaag
      301 tattgtttgc agctgtgttg ccagtttcta tagataaatc acagcttttg aaacataagt
      361 tttgtactgc ttcgattcgc ccgaattctt ttgcagttat ttggcaatga gaaaagcaga
      421 catcttctat aggcttttca ggaaatgcta ctaaatcaaa agctcgggaa ggaagtgagt
      481 aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat gtctttgact ttcgtcattt
      541 gctttgattc aggtatttct tcagccactg cttgccaact ttctggcatt tgagcaaggt
      601 cattgctatt ttttctattg tattcattat gccagtccat tatccaagaa aaaggaaatt
      661 gtacatcaat catattcaga ttttctaccc tgatattttc gatcactccc cctctttctt
      721 ttgaagattt cattcgaata ccacagtctg actgaaaaaa atcgatatcg tgaatatata
      781 cgtcactaac tccagcacta acttcgctac caatagttac accgtatcct gaacggattt
      841 tacatctagc aacctcgatt cttgatgatt ttcgattgac tcttgcacca ttgccatctc
      901 tgccagactt aatagcaata caatcatcac cacaggccaa atcacattca tagacacgca
      961 catttgttga agaatcaata tcaatcccat ctgtacttgg tccatcattg ttgcggatga
     1021 ttactttttc tactaataca tcgttagaat aagtgatttg caagttccaa aaacctgatt
     1081 tctgaaaaat caaatcacga acaacaacat gttctgcatg atacagtaaa caagcgcgtg
     1141 gacgtttaat agcataatcc gcaatccaac gaagtccctt ttgatcgtat tcagctcttg
     1201 tcccgctttt ttgatctttt ccccaataca gctcccacca atggtctcct tgaccgtcga
     1261 ttattccttt tccttctata agaatatctt tcgccgagag aacattcaaa atcgctgctg
     1321 gccatttcat ttctacgccg gctactcgtg tatcgatttc tggaaatgct tcgatatctg
     1381 ttctcccctt gaggactgca ccttcttcaa agataagatg actcttatct tttaaaaaaa
     1441 gggctccgac taaataaatg ccttttggaa taaatacagg taagtgttct ttcgcacctt
     1501 gatcgattgc tttttgcagt attattgtat catcttgttg agtgtcgttt gttttaaagt
     1561 tcaaagcatt gataatccct ttttttgtcc gcaatacgtt tctccctatc tttcattgat
     1621 ttttttcaat tcttcaccaa taaatacagt ttttgaacga ttcagtgggt aacctactag
     1681 caaaatgatt gcggatacaa ataagcagaa tgctggtaac aagtttgcca tcgtataaat
     1741 cctttcttgt actaaaactg attggatggc tccacctgtt gtagacgatt ggtaaccaat
     1801 gagtgccagc ataaatccgc ctaccgctcc tgcacatgct tgtcctactt ttctcgcaaa
     1861 agaatttact ccatatactg ttccatcttc tcttaagcct gtcttatatt gatgataatc
     1921 aatcacgtca gtaatgaatg cccagaccat caaattgaat aaaccagatc ctaaagtagc
     1981 gataaataaa agaatcagat atacccatgc atttgttata tgaaggaaat acatcagcaa
     2041 gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct tcctttttac caaataattt
     2101 tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt ccgtaagtaa ataaaagagc
     2161 aacagacatt gcttgtttat tatggaagta atcattgaat aaataagtca tattcgtccc
     2221 agctaaaatc tgattgatca caatgaaaat gtcaacaatg actaaaataa tcagtgctcg
     2281 attagctaac aaacctttta ccaactgaga agcagatact ttttcgtttt tgacaactct
     2341 tactctttca gtagtcaatt tacaagttaa catatatgcg acaaatgcta ttgcagcaca
     2401 tccgattgaa atccaaaaga aatgattccc tgaaacgact tgttgtccac tcttgttatg
     2461 ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat ccgccaatcg cagaaccaat
     2521 gcttcggaac gtggacaacg aggtgcggtc attaggattt gagctaattg ctgctgccat
     2581 tgctccatac gggatattga ttgttgagag aaaaatccca taaacaatgt atgaaacaaa
     2641 aatataagca atcttcaccg tcattggaag atgttggacg aatggcaaaa acaaaatgac
     2701 agtcactaag caaaatggat acttcatttt tctgatccaa ggaataaatc ggccagtttc
     2761 tgttaactct gagacatcac ataatcttcc aaccatcaca tcagcaaacg catccaaaat
     2821 tctcgaaatc aagaataacg tacctacgat tgcccctgaa acgccaagaa cattcgtata
     2881 ataaatcatc aagaaactat tgactaaacc taaaataaaa cagttaccta gatcaccgaa
     2941 catgtagcct atcttatcgg aaatcccaaa ttcctttttt tcttttttta ttgtatttgc
     3001 tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc tcttctagta aacgttttca
     3061 aaattataac tctttttttc ttgtttttat attcacaatg ttggcttttt attgcattat
     3121 cttgacagat aaaaaacaaa aaataatcta aaaacattga tgcataagat tgataacttt
     3181 ttttacataa caatatatac aaacaaaaaa attcactaac tagtgaaaat aagttagtga
     3241 acctcattta ttaaagaatt aaattacaga tgatcttttc catcatactt gaaaaactca
     3301 aaatcagcat aacttcctct ttttttattt agatcatgca ctgctatccc gataaaattt
     3361 ccagtaaacc ctcctgagag tatacacatg tttttttgcg gacctacttt cttccattct
     3421 tcagttgata cgtctttcaa atgaaactgt gctgtctctt tctttacagt gactttcaaa
     3481 aaagtcttct tttctgtgag tggaatcgtc caatcttcta tttgtgctac tccgttttct
     3541 gattctatca tacgtaggca ttttcccttg tcctcattcc aagtaacata acaataaata
     3601 taattggatt catttaagta cagtaccagt ccagccatct gattaaaatt attaggcatg
     3661 tattcaaatg ctgtctccgc atcaaaagta aaatccgttt gccgaatagc taacaaatga
     3721 tgttcgaagg tagattgaag agattcaccg gaaatcagac gtaaaaaacc tggtctacat
     3781 tttgtatcac accaatcagc atttggcaaa atgcgtctgg cattccagtt cttctttagc
     3841 tctccttcaa atgtatccat aaactgatga tctttttctt gttgtacctc ttcatttgtc
     3901 tcaatcatta tttcttccat cggagttgtc tggctatctt taagtcttaa ccaatcgtcg
     3961 gacgtccaat acacttcttg aatagcggtt tctcttccca aaatagccgc accgtttaat
     4021 ggtctggtac aaagatatgt catgtaccat cttcctgacg gggtttgtac taagctccca
     4081 tgtcctgtac attgcaatgt agaagcaggt ttgtcactag ctgtcagcat aggaaaacca
     4141 ggatcaagct cgtaaggacc agtaatattc tttgctcggc aaactgtaac tgaatgtcct
     4201 cttcctgtac cgccttctgc cgtcatcaaa taataatacc cgttacgaaa atacaaatgt
     4261 ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt tatagatggg accatcaagc
     4321 ttcttcttct ctgcatcata ttcttgtaat acgataccgc tggatttatt cggtgtgtcc
     4381 agacgataat cccatagttc attgacaagc catttttttc ccgtttcaag atcatggaac
     4441 aaagaaggat caaagccgtt actatttaaa taaattggtt cggaccaagg acctgtgatc
     4501 tcagatgcag tgatcaaata attgtggaca tctttaaacg gacggcctgc acttttgaca
     4561 tctgtgtaaa caagataaaa caatccatca gcataactga tctgtggcgc ccatatactt
     4621 ccatttgtag gatttccttg caaagatact tgagtggtca aaatatccgt tgtatgtgac
     4681 cagttgacta aatttttcga ctcatatact cggattcctg gaagccattc aaaagaggag
     4741 acaataatgt aataccgctc tttcactctt aatatattag gatctggatt gaatcctctt
     4801 aaaataggat ttttcacttt gatagtctgc atgattcagg ctccttttct cattcttact
     4861 cgatataaac tgaatgcccc agctgcgaaa taaaaaaatg caaaaggaaa cgcaataaac
     4921 tgagtgccaa tcaacaatat gaatcctgtt ataaaataca aaccagtggc agttgttaag
     4981 tattttcctt tagcagtcaa aaacaatgct actgctaaaa aagcaagtga aataaacaac
     5041 acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc taaagacaaa
     5101 gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc agacatatta
     5161 cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc gaccattcct
     5221 agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa tgtatccatt
     5281 caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg acagtctgtt
     5341 catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca cctatcttac
     5401 acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag acgtttctag
     5461 tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt tgatttttcg
     5521 gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg atatgacact
     5581 gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca atccagcaat
     5641 attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa aaaggagtac
     5701 catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa ccagatagtc
     5761 gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc tgtcctacct
     5821 tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca ttcgatcctt
     5881 ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga tactcctgtt
     5941 ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta aaattggata
     6001 atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc tttggatggc
     6061 tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa tccgtcgggg
     6121 tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat gctcccttat
     6181 gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg ttaaagaaaa
     6241 agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc cttttctcag
     6301 cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc taagacgagg
     6361 gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata gatttggcac
     6421 atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtattt cgatgtccac
     6481 ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca cccaccagct
     6541 taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg tgtcttccct
     6601 tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc ataacgtttt
     6661 tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc gttcttcaaa
     6721 tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt ctgcaaaaga
     6781 tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg aaaaatctcc
     6841 gccgtgagaa tgcaggtaaa ggaccggcgg aaacggtcct tctttttctg gataagcata
     6901 gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa gcataaaacc
     6961 gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag atggagcacc
     7021 taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca tccatatttt
     7081 ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct aatagaaatg
     7141 ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc gcaaagccaa
     7201 tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga acaccgggaa
     7261 ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt atgaattgtt
     7321 ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg atattcgaaa
     7381 aatggatcct tctgaatgct ggtgtccgtt catctatcgg ataagccttt ttttcccata
     7441 catacggttc tttccctcgc ggaccacaga aataataaag attcagtata aatggacaca
     7501 tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg cctcgacctc
     7561 gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt gaaatggtaa
     7621 tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt ccatggacca
     7681 tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta tcttcagtcc
     7741 ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta atacgcacat
     7801 ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg ttcaagatcg
     7861 tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa cttggtgact
     7921 ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc atcaatttag
     7981 gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat ttttttccat
     8041 ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga gcatagatac
     8101 aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca ggataatctt
     8161 ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg acattgcttt
     8221 ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc ccgcctccgt
     8281 cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca tcaattgatg
     8341 cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca atgcttgcaa
     8401 gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt agtaagcata
     8461 tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt tattcaagtt
     8521 cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat gtgatttctt
     8581 cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt tagccattgc
     8641 gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt ttgtttctgt
     8701 tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg cagccagtgt
     8761 cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta attcataggt
     8821 atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat gggatagacc
     8881 tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa tcagcaaatt
     8941 tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa catcttggta
     9001 atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac ccatatataa
     9061 gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc cttcggatgt
     9121 gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat atttttcttc
     9181 gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt cgatattgta
     9241 ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt cgatattgtc
     9301 ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt ggaacccttt
     9361 aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt catataagcg
     9421 aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca ttatttgctc
     9481 tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt cccaatcgtc
     9541 tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt cgatcgcctc
     9601 cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc tgtcccatga
     9661 tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact tcattgttcg
     9721 tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt tctttgatca
     9781 attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc acttcactag
     9841 gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg ataaattcga
     9901 tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc atcaatttat
     9961 acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat tgtgcttcgt
    10021 cttttacttg agatttggga aatgctagca agccgccaat accatttcct tctgaccaaa
    10081 catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat tccatatcat
    10141 cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca gtgtagattc
    10201 cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact gcaaagtctt
    10261 gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc atatactctt
    10321 ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca tttggtgctc
    10381 caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc gcacgatcgc
    10441 caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta aattgtttag
    10501 ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta tccaaccagt
    10561 cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg ccgtacaagg
    10621 caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa tttttatagt
    10681 cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg cggacagaag
    10741 agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca gaagccaatg
    10801 cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc agttccgtat
    10861 cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca tgatgcaaaa
    10921 tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg ttacttgaac
    10981 tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat tttacgagcg
    11041 ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt ttcttctgtt
    11101 ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc tgtccacgtg
    11161 actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat tgtttggatg
    11221 atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg aagatgtgag
    11281 aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc ataatgagcc
    11341 atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat atacaaagcg
    11401 atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat cccgatggcg
    11461 ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata tgtgaagtac
    11521 gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac tgcaaccatc
    11581 gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa accgccccac
    11641 agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat tttatctaat
    11701 gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc tcctagcttt
    11761 ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt aaacaatccc
    11821 attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat ataggtgtcg
    11881 aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg ctcgaaccct
    11941 aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg acgaattgcc
    12001 ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc tgcagcttca
    12061 tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga ccagcccaac
    12121 tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga tgttagaaaa
    12181 ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc gtcaacattc
    12241 aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg gatgtaaatg
    12301 atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag taaagacaaa
    12361 atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa cgtatttctt
    12421 aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa accgacccaa
    12481 ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac gagtcccccc
    12541 ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat cacatataaa
    12601 aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt ttcttctttt
    12661 ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag cccctcctta
    12721 gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa ttacgtatat
    12781 aatcattttt accgtaagta aaaacgctta catagcaatg atttttgtgc tatacaaaac
    12841 gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt tttttctatc
    12901 tgacacttag cataggaaat gattatatac gtaatctttc accgatgata aattgaacat
    12961 atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga aaaagaaagt
    13021 ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt tgctagcttt
    13081 gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta cacaagaagt
    13141 cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt atcgctacat
    13201 tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta caggtgtcgg
    13261 aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca gaaagtattt
    13321 gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca gcggggggat
    13381 gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact gggccatgat
    13441 cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt tccaagcctt
    13501 gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg gtgtattttt
    13561 caaaattatg ctgccattag cgttaccgtc tattgctact atctcgcttt tttatgctgt
    13621 tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat ctaaatggcc
    13681 gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag cagaaagtag
    13741 tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg ttattgcaac
    13801 agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag gagcactcgt
    13861 aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt cagaagtggt
    13921 cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt gtgaaatttc
    13981 ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg tgaaagagac
    14041 gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa cccacaggag
    14101 caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg ccttattgtc
    14161 gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa gacttttgtc
    14221 acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca aatactaggg
    14281 ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct cttgattcac
    14341 aaatattttt ttcgatattc tcccggcgta atcgattcct tctttttaaa gaaacgaata
    14401 aaattttgcg gatttcgata ttgcaatctt tctgcaatct cttttacgga gaggttagtc
    14461 tctttcaacc atttttttgc tacctctaaa cgatgatttt gaacaaaatc accaaatttt
    14521 tctcctgttt cctttttaaa tacattactt aaatagttag ggttataatg caacctatca
    14581 gcgatcacat ctaaagacag atcacgatca tattctgtgt agacgatatg cataatattc
    14641 tccgacaaat ttttaaattc atgatcagtg attgcttgag agcattctgt gattggcaga
    14701 actaattgtt ctaaaacaag tttttctagt tctttgggat gataattgtt cagtgctttt
    14761 acataaattt gtttcatgtt atcaaagttt tttgaatcta ctcccaataa ctgtcctaat
    14821 tgaacgagtt cattgatcaa gcggaccgaa actacttccc gactcaaagg atttttattt
    14881 tgtgcaaaca atgcatcaat caattcatgt actaaattct tcacttcctg atttccagaa
    14941 cgaatggctt caaacaatcg attttgttct tctatcggat atctaataag tgttttttca
    15001 tgagcagcag gaacgatttc ctgataaaaa ataattgaac ttggaccagt atttacacga
    15061 taatacaggg cttcttttgc ccgatcaacc gattgcttgc tttcattcag cgtttcgaac
    15121 cggttactga tgcctatact gatagttaaa ttcaagtatt cttttacacg tttttgaatg
    15181 agtcggcaat actccatgac ttttttgctt gcttctgcat catctttatc cattcgataa
    15241 atagtggctt gcatttcttc attcaaaacg atcggcagca ttcgattttc ctttggcaca
    15301 atttccgtga tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc accgtgacta
    15361 tcttctaaca gatcgatttg aaccaaagct gtataatagc agtcattttg agaactataa
    15421 ccaaattgct gcaaccgctg atctagttct cgcctttcta ctcgatttct aaataaacta
    15481 agtacaaata gcgtttccag ctgtggcttt tgggtaacta agtgtgcact taacgcttct
    15541 ttttctccta tgatccgact gactgattga gcgaccaaat ctagttcttt cccactgaat
    15601 ccttttgatt taagattcag acgttcctgt atttgagaaa tcggacgggc aaatctgtcc
    15661 gaaaataagt aggaaagaag accaatgagt cctaccagtg ttaaagacac tgcaactagc
    15721 ccgattctca gattgcggat cattgcgcca attgcactag agtcaacttc taatctgtaa
    15781 atccaattat tataatctga tttcagtact atatacgttt tatctttttc tcttatcact
    15841 ttcagctgat tgtaatctag atccacgtcc gttaatacag ttggttgctc tttatttttt
    15901 actgtctgag tagtaaaaag gttgccaact tcgttttcaa tagaaagtat gtcttctcca
    15961 ctttcaccaa caacttgttc tattgtatgt ttctctatcg tagcaattcc caatgcatat
    16021 ttttcttttt caaacattgg caaagaaatc accatttcga ttcctttgtt tttcttttgc
    16081 caaaataaat tatttttgtt atttatgtac ttctgttgat acttttcaat ttcctcttcg
    16141 gtcagttgac tcaacgatcc attgattact ccccatttac cagccaaact gactaaatca
    16201 taattattat catccataac aatcgtttct ataaaattta gttctttcct gatttcagaa
    16261 taagtggagt aatctgtata tgtcagcgga tgcttgaata tgtccgagaa atttttggtc
    16321 gaaccatacg tagaaaacga atattcaatc gttctaattt tccattcaac gttagtttta
    16381 acttgcctga gcaaattctt tttgtctgtt aaataactcg attcgatcga ttcataggta
    16441 ttctggtaaa tgaataaact aaatcctatg acaagtacgg ctcctaataa aaacatcggt
    16501 aaaaatattt tataaaatac tgtacttttc ttcattttct tcctctcctc cttttaaaat
    16561 aaaaggcttt catttttctg taaaattgat actcatctaa ttaaatgagc attccccgct
    16621 ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt gcaatctttt
    16681 catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa
    16741 agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatgtcgct aaagcagctg
    16801 gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa
    16861 caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa
    16921 aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag
    16981 gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa
    17041 attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac
    17101 gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc
    17161 tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta
    17221 tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat
    17281 gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac
    17341 gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ccagcctatt
    17401 ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac
    17461 tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga
    17521 gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg
    17581 attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag
    17641 aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac
    17701 aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa
    17761 ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat
    17821 gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt
    17881 tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca
    17941 acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat
    18001 gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat
    18061 cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg
    18121 ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac
    18181 agaggcagga atccaattcg tggagactga taatttaaca gatactcccc cacagagcct
    18241 cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac
    18301 catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg
    18361 ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt
    18421 tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt
    18481 tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct
    18541 ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt
    18601 gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa
    18661 tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga
    18721 agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat
    18781 tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg
    18841 tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa
    18901 gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc
    18961 accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa
    19021 gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac
    19081 agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc
    19141 gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc
    19201 agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga
    19261 aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac
    19321 tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg
    19381 cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg
    19441 tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt
    19501 ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga
    19561 accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt
    19621 ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca
    19681 aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag catccttatg
    19741 gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa
    19801 atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc
    19861 ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc
    19921 aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg
    19981 cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg
    20041 gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact
    20101 acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag
    20161 aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga
    20221 tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc
    20281 ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa
    20341 aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag
    20401 ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct cttgcttcat
    20461 ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc tcatatgtcc
    20521 caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat tggatggatt
    20581 ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg tttgttcaaa
    20641 aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac gttcgagaaa
    20701 ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata aaaagaaaga
    20761 aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg cgaaaaagct
    20821 ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact tagatccaaa
    20881 agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag gaggcgatca
    20941 ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca caggaaatgc
    21001 ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta tcggaaatcc
    21061 gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact tgttgataaa
    21121 tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag ctcctgattt
    21181 tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa cagatgatcc
    21241 cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt tgaaggttct
    21301 accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta atgcatgggt
    21361 cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac tgatagaagt
    21421 cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg accacgcttt
    21481 agacatctta cgctataaag aagcggatga aacagtatta gaagagattt ttcaaaaagc
    21541 acgagctaac aaactattat cttcagatga aatcgatgct tatcgtacag aaacactgat
    21601 acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca tccatgctta
    21661 tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct atgatggaat
    21721 gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag aagaaacgga
    21781 tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg cgattttagc
    21841 tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt ctggatggtg
    21901 gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg atggtagtgc
    21961 tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct acactagaca
    22021 tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc gaggagaagc
    22081 acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata caaatgctaa
    22141 aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat caaaaacagg
    22201 atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga ccaaatttgc
    22261 tccgtcttct tctcttgctg ttctgcttca ttgcctataa acctttatga aagaagagat
    22321 aacatgccta ctatccatat tgccggtgat tctactgcgg cagaaaaaga tgaaaacaag
    22381 cgtcccgaga caggttgggg agaaaaaatc tctgcttatt tttcacccga gatccagatc
    22441 aataaccaag caaagaatgg acgcagcacc aaatcattta tccaggaagg aaggttagcc
    22501 cgtctagctt cacagtttca gccgaatgat ttcttatttg tacaatttgg acataacgac
    22561 cagaaaatcc aagaagaaag aggtacgctt cccttcggta gctatttaaa aaatctgcag
    22621 atattttttg aatgcgcaaa aaaagcgaac gtccaaatgg tcttactgac atctgtgacg
    22681 cgacgagatt acttagaaaa cggaacattg aatccagata tacttggaga ctatccaaaa
    22741 gcgatgcgtg catttgctga gaaacaccat attcctcttt tagatgtgtt ctcaagatca
    22801 caagaattgt tccagacctt ctcaaaagaa gagacaaaaa aattctacct gcatcttata
    22861 cctgatactt gtaaaaatta cccagaagga ctaaaagaca atacccactt tagtccagaa
    22921 ggcgctgata aagttgcccg aataattgtt gaattgataa aagaatcccg tctgcctctt
    22981 gctaattatt tacaaaaagg agtttgagat gtttccatta aatttaggca tccgtgccca
    23041 cgatttagat gttcgaaatc gtgaagaaat cgttcaaaaa gtatcacagc ttcacttgtc
    23101 ccatatccaa tttgcaccac aaaaagcatt tcctgaacaa cttaaattaa ctgacatgac
    23161 acttgggagt gctgcctatt ttggtgaata cttccaaaaa aacaatatcg aactttccat
    23221 tttaggatgc tatatcaacc tatccagcaa aaatcaagaa gttcgtgcac aagcattggc
    23281 aactttcaaa cagcaaattg ctctgtgctc tgcctatcaa gccaagatga ttgctacgga
    23341 aaccggtagt gtctctgtcg gttacacaaa agaaaatttt actgaagaag cttatcagat
    23401 cgccagaaat tctatcattg aattagtcgc tcatgcagaa aattttggcg taactgttgc
    23461 tgttgaagca gggatcaatc atccaatcta taactatcag ttagctaaac gtttaatcga
    23521 tgaagtagct tcaccaaatt taaaaatcat tttagattgt gcgaatttga tgcataaaga
    23581 aaatcatgct gatcaggaaa aagtgatccg aggtgccttg gacaatttac atggttatat
    23641 tacagcgctt catctcaaag actatatcat ggaaaatgga aagatccgca tggtacctgt
    23701 tggcaagggc tgtttagatt tccgcccggt actgcattat atcaaatatg agcgtcctct
    23761 aatgtacgcc actcttgaag ctactcctga gatttatgtt cctgaagcaa tagaacactt
    23821 gcagtttatt taccaagatt gttaaggaga agattatgga ggaacgaata cagaaaagtt
    23881 atcaaaaata tcatgcaaat gcttttcaga cattgctgca tctttatcag ggaaattatt
    23941 ggcgttttgc catgtcagga ctctttttta ttatcaaaca tcttccatct tggttaatgc
    24001 cgatcgctat tgccaatgta atcaatgcag cttcatcgaa agattcttct cagttgcagg
    24061 ccatttattt caacgctttt ttgatggctt gtctgattgg tcaaaatata ttgacgaatt
    24121 attttcatgt aaaataccat agcacttcga tccgtcaagt agaagctgga ctaagagcga
    24181 cccttatcaa gaaaatccaa gaactatcta ttccttatca aaaagaattg caatctggac
    24241 gaatccagtc aaagatcatc cgtgatgtag aagctatcca aacgttgtcc tctcaagtat
    24301 ttgtcagcgg attaaatatc gctgtcaatt tattcgtagc tttaggtatc acgctttata
    24361 aaagtcctgt tgttttcatg tttttcttat tgactgttcc tctcgccgca ttcgttactc
    24421 tctttttccg taaaaggatc agacagtcca actcagactt caggcaagaa atggaagaga
    24481 cttctgcaaa agtgatcgaa gtgatcgaat tagcaccgat tgctcgagct catgggttag
    24541 aaaaacacga aacaactcgc ttaaataagc attttactca aatctatgaa aaaggattcc
    24601 acttagatat cctgcaatct gttttcggat cagtcagctg ggctagtttt cagatatttc
    24661 agctgatttg tttgattttc actgggatat tagctttaca agggcgcatc caacccggcg
    24721 atgtcgtgat gtatcagacg tatttcacga cgatcgtcaa ttccgtttct ggaatgatca
    24781 cgctgattcc tacgatttca aaaggcatgg aatctatcac ttctattggc gaactgttga
    24841 ttgctggcga tgtagaaact tatcacggta agaaaaaaat ccaacatttg gatggaaatt
    24901 ttcgttttga ggatgtttct tttctttatc cggactcact caaaccgatt atcgatcatt
    24961 tttctctaga ggtgaaagct ggagaaacaa ttgcttttgt tgggccatct ggctctggaa
    25021 aatctacact ccttaactta ttgattggat ttatccagcc gacttcagga aaaatttttc
    25081 tagatgatca gcctttggat gaactggata tgcgaagtgt gcgaaattat ttagccgttg
    25141 ttcctcaaac cactttgttg ttctccgctt cgatcaaaga gaacatcact tatggcttga
    25201 aaaacgtatc aaaagaacgt ttagatgaag tcatcgaagc tgcgcaactt tcttcactga
    25261 ttgaccagct tcctgatggt ctggatacct tagtaggcga acatggcaac aagctgtctg
    25321 gtggccaaaa gcaacgtatc tccattgcac gagcgttgat acgtcagcca aaaatcattt
    25381 tgctagatga agcaacctca gctttagata accagtcaga gaaaaaaatc cagcaagccc
    25441 tgaattacct gacagaaaca cccactactt ttattgtggc tcatcggctt tcaacgatca
    25501 aagaggcaga taaaatcgtt gtgatcgatg aaggaaaaat cgttgaaata ggtacttacg
    25561 aaaaactgtt agctcaaaaa ggctatttct accgcttgaa aaatgaatga caaaaaatag
    25621 gagctatgac aagacttttg tcgtagctcc tatttctgaa taaacggtgt tctcctgcca
    25681 actcctcggt cacaagtcac aatagtcatc aaacatgaga agctgttttt tgaaattgtt
    25741 ccttgtaact cagagttaaa cggtaggctc acgacctctt ggtaaatggt gttcaagaga
    25801 cagcctatcg gcaataagaa gcaattttgt catcgctcaa ggccgaacgt ctctttcaca
    25861 ccctctgttg tttcataaaa tagttttatt cgctgtaaag gtcgcgaact ggtgtcatga
    25921 tgatacggtt caagtcattg atgattgtac tgaagccttg ctctttttgc atcaagtcat
    25981 tgattacttc ttctgattgg attttcacag ccatttcttg tgctttcatt gcatcttcgt
    26041 cgctgaactc ttgtccactc atttgttttt cttgtaattc gatttgcatc gcttggaagt
    26101 ctttgaacag tttgtgtgct tcttcgtttg cttgtacttt ctcgtacgct tctttcaacg
    26161 ctttgaattc tggtaattca cggatctcac gttcgatttg attcgcgcta tcgtagatgt
    26221 ttgacatgtt atgccctctt ttcttttagt agttactctc tattgtaaca tggggttatt
    26281 cgttttcaaa ggggctactc tccttgatat ttccgccaga ggtctttagc cgcttcacca
    26341 attccctgaa cgccttcttt caccatatcg ccgaagtttt tcagcccttc ttttgcttgt
    26401 tcaccatatt gattcaagtt atcttgccaa tttccagact ctccattaga atttcctgtg
    26461 tttccaactt gatctgcagc taccacttgt cctcctgtgg cataggcatc tgctacacta
    26521 aatcctgttt ttgaagtata cggaaggatg ccttctgccg ctgatttgaa gactttcccg
    26581 acaacatttc cacttgtccc ttccaaatag tgcagcttgc tggattcttc gtaacccatc
    26641 catgtcgaaa tcactacgtc tggcgtgtaa ccgatcatcc attggtcatt tgcttttgtg
    26701 gcatcgaaat tagtttctgt ggctcctgtt ttccctgcta tcgtataacc tgcaggattc
    26761 gcagcagccg ctgttccatt tgaaaatgta ccaagaagca tgctggtcat ttcatcggct
    26821 gtttcctttg atatgacctg ttttggtttt ttgtttgtgt tatcgacgat caccgctcca
    26881 gtcgaatcaa cgattttcgt gataagatgc ggcgtataca tctgaccatc attggcaaat
    26941 gcactgtaag cacccgccat gatcaatgga gatactcctt tttccagtcc accaagggct
    27001 aagccataat acttatcaga ttttgctaaa ggcaggccga attcttctgc tttatcatat
    27061 cctttttgta aaccgatctc atgcaaaagc catacagtag gcaaattcaa actttgtgca
    27121 acagcttgat acatcggtac ttctccttgg tatgtcccgt caaaattatg cgcatcgtaa
    27181 taactttgcg gttcgtcttt caagatactg gaaggcttgt atccagcttc cagtactggt
    27241 gtatatacgc ttaatggttt gatcgttgat cctggtgaac gacgcatctg tgtagcaaag
    27301 ctgaatcctc taaagacatg atctcctctt cgtcctacta gtgcttgtac accacctgta
    27361 ttcggatcta aagctactga agcagattgg accatggcac catctgaagc atttgctggg
    27421 aataatgcac tgttttgata agtagcttct aattgctttt gataatcctg atctaaagct
    27481 gtatagattt tatagccttt attcatgaca tcttcttcat caagaccata ctcgttaact
    27541 gcttcatcga tcactgcatc aaaataataa ggataacgat aaccagaatc tgaattatcg
    27601 tatgtgtcat taagatacgc gctgatatct gtctggcttt ctgcttgttc ttgttctttt
    27661 gtgatttttc cgttttcttc cattacactt agtactgtgt ttttccgatc attcgcattt
    27721 tttgggtgat cgatcggatt ataaatacca ggacctttca gcattcctgc caaagtagca
    27781 gcctcagaca gcgtaacatt cgttgcatct actccaaaat actttctgga agcgtcttgg
    27841 acaccccata ccccgttgcc aaaataggca ttgttaagat acatggtcaa gatttcttct
    27901 ttgctgtact tcttttcgat ctcgattgcc aaaaataatt ccttcgcttt tcgatcaaac
    27961 gtttggtcta atgtcagata cgcatttttg gcaagctgct gagtgatcgt acttcctccg
    28021 ccccctgatg ttccgccaga caagaccatt cggactgctg cacgggcaat ccctttgata
    28081 tcaaacccgt gatgctcgta aaaattccta tcttctgtcg agattactgc atcttggata
    28141 tagggagaaa tcgcgtttaa ttctacatat gttccttttt gggcgtacag tgtgccagct
    28201 tcttcgtttt tcttatcata aatgactgtc gattggctta atcctgattt taacgtttcg
    28261 acatttgctt gttttgctaa aataaagagg tagatacttg tcactaagac acagatcaaa
    28321 ccaatcagca aaatcaattt gttcacatga tattttttcc agatgcgctt tcgccatcga
    28381 tggaattgtc cgagatatgg tctgatccaa cgccaaaaac tgatcaaagc agcttttatc
    28441 ttttctaaaa tgctttgaaa atccatttgc ttcttctcct gtctatttga ttcaatcgtc
    28501 ttattttacc atagtgaaag agtgttttct tcctgcttaa gttgtagttc aatatgtttt
    28561 tttacgttag tgcaagtaga gtaacataag aatgaacaga aaaatgtaag ttttttctta
    28621 aatatgtttt aaaaatggta caattagtct gcgattgcac tgaaaggagt acacttgtga
    28681 aaatcacgat cactttacct atgaaccatc ctgaaacaac aattcgcgac ttgcttgaaa
    28741 aggaatggtt agttcctaga aaggttcgac actttttgcg tatacgtaaa aacgtttgga
    28801 tcaatcaaca acctgctttg ttccatcatg aagtaaaggg caatgaccag atcacattgt
    28861 tattcgaaga aacagattat aattatcaag aagttcaatt aggagatgcg aagcacatcc
    28921 gtgttttata tgaagatgag catttgatta tagtggataa gcctgcagga atgaagactc
    28981 atccaaatga accgacagaa aacagtacct tactcaatca tttagctgct tatctcgaca
    29041 ctaaacagca acgaccttac gttgtccacc gcttggacaa agaaacaagc ggtgccgttt
    29101 tatttgcaaa aaatccattt gtcttgccga tacttggaag aatgttggaa caaaaaatga
    29161 tctatcggag atatcaagcc atcgtatggg gagaaattgc caaagatatg accatcacca
    29221 ataaaattgg acgagatcgc catgatcgga gaaaaagggt cgtagatgat aaaaatggtc
    29281 agagcgcctt gacacatgtc actgtcaaca aagtactttc tgaacaaaca caagtctatt
    29341 gtgttttgga aaccggacgt acacaccaga tcagagttca tttagctcat aagggacatc
    29401 cgatcgttgg agaccctctc taccaaaata gaacagctga acgactgatg ctccatgcat
    29461 tagaactgca catgatccat ccatttactc aagaaaaaat agtcggtaaa gctttacctg
    29521 gattatggga atagaaataa aaaagaacag agtcttatca gcagataatg ataagactct
    29581 gttctttttg tgtaatttgt ctgcttactt ttgatgttcg tgttctgcta acagcaaact
    29641 accgataatc ccactttcat tcggcagtcc ccaacgaacg atatattcac ttacttgagg
    29701 tgtttccata taaccaccca tcaattcaac aaattgctga cggatttttt gaaggagatg
    29761 atcctgattc attaccccac cgcctaaaat aatcttttca ggtgctaacg tcaacgtata
    29821 gttgatcagt gcttgagcaa tataataagc ctgaatagcc caaacaggat gatcttgcgg
    29881 cagatcttgc cctttgatcc ctgtgcgtgc ctcaatagac ggtcctgctg ctaatccttc
    29941 taaacaatct ttatgataag gacaagtacc ttcataatgg tcatctggat ggcgtttcac
    30001 ccaaatatgt cccatttcag gatgtgcgat ccctgaaaaa atttcttcat tgatcacgac
    30061 acctgcaccg attcctgttc ccacggtaag gtaaatacaa ctattttttc cttgtgctgc
    30121 tccttgcttc aattccccat atgcagcagc attgacatcc gttgtccaag cagcaggaat
    30181 attgtagcgt tccttgattg tgccaagaaa atcgaattgt gcccaccctt ttttgggagt
    30241 tgccaaaacg tatccgtaat tatcttttgt tttgtcgata ccaatcgggc cgaatgagcc
    30301 aatcccgatt cccttcaatt cataacgatc aaaaaattca aatacctgat ccaatgtttc
    30361 ttctggtgtt gtggtaggga tacttaattt ttctaaaatt tctccaccat ctgaaaccgc
    30421 acacacaaat tttgttccgc ctgcttctat tccgccatac attcgttttc ctcctcgaat
    30481 ccgattgtcc gaatctcata aggttggatt attgggacaa ccgcttgctt cgttggttct
    30541 tcaagcagat tcaatatgac agtttttcca ttgtctttag ttatttccaa tttttctaaa
    30601 tgactagaca tattgaaacc tcttactact actttatcac taaacttgct gcgtttcaaa
    30661 gctgtaattg caaacgtttc actttttatc gttaaatatt gttgttttga aatcaatgtt
    30721 ccttcatgat gtttgatttg ttgagtggaa aaaggaatct gtgctgcgta tgcatgcaaa
    30781 taagtagaga atttttcttc tggtccatgt agttcaatgc tgtaattgaa ccgatgctca
    30841 cctagacact gtgcttctgg tgttggaaaa tatccccaat cgcctagttc tcccacactg
    30901 cgaagtagcg tcactgcgat ttgtccgtct tctgttactt catattcatt caaaccaaag
    30961 tttccgactg ttacaccgta ctcttcatca tgaatattga caaatgcttg ctgatgttgc
    31021 ggattcgtgg ggttttccca acttttagac acatggtttg ggcgcttcac cacttcaaaa
    31081 atactatctg cttcgtgatg ttccacatgt aactttgtag gaaacaagac acgtaagcga
    31141 tgatctttca tctgattgtc gagagtcgtt tcaaagtcga tttttttact atcttttctg
    31201 attgtcatga ttgtttttat ttgaaggatt cgtttttctt tggatcgttc tgcttttcga
    31261 taacgaaatt ccacaaccat ctgctgttct ttttctaaac gctcgtctgc agaaacaggg
    31321 atttccattt cctgtagaat acttattttt gctatttccg gtgtatctac taatactttt
    31381 acttcagagt tttctttgtt actggatagg attggctgat cacagaaagg ttgtttaaag
    31441 atatactcat ttcctatatc gccgatatct tcaaaaatat gcagattatt caactttcgt
    31501 ttgtttttgc gatcttccat tgtaatggta ccattttttt caatcgtcaa atgtagaggg
    31561 ccgttttcga tgattcgtcc actctgatga atcattgttt ccttttcttc tgctttcgct
    31621 tccccttcta caagcgcgaa tgtttcccaa gaaaatgctg gcatgttttc taatggtaaa
    31681 gtcactttga tcattcttgc catatatggc acacggaaac ggtctttcgg caaatcataa
    31741 ccaaattgga cagtttcttc agataaaata gctggtatag tggccccact ttttgtttct
    31801 acatgatact tacgtttagg caaattttct aactcttgat atagttgttc aggtattcct
    31861 tcagcgaatc tttttctttc taattcgatg gttatttctg cttcaccagt tttatccatt
    31921 cctgcagtat ttacaatgac aaatggaaag ctgtcttttg ggaatccggt cgtatcgatt
    31981 gctgcggtca gctgttctaa ggaatcttgt gcaagatact ttccaacttc atctgctttt
    32041 tcaaaacgag gtatcatttc ccgatggaca gaatcgactg aacaaccgca aatactgtcg
    32101 tgaggatgat tttgcattaa tgtcttccaa gcataatcta attgatcatg gggatagttt
    32161 ccactaactt cataagccat cgtcgcaagt ggttctgtaa tattttctag ctgtcgctgg
    32221 accttggtat tccattgttt taagtaaacc cgagcagatg ctgtattggc taaagtatac
    32281 cagccatccg tttcttgact tgtcaattct ccttctactg agcctaagtc ttctggcaca
    32341 tctttttgaa ctgcttccag atagtcagta aaattagaat gaataaactc gtaatctgga
    32401 aacagttcat ttgcgagatg gattgctttt gatatatctt tttgtaccgg ttgatgatca
    32461 acaccattca tcatcaataa gtggttcgta gaggcatatt gttctgcatc cgctaatttt
    32521 tgcttccaaa aggcaagagc tgcttctttt tctactggaa tctcattccc gttgctatac
    32581 caattggcaa atagcagacc aaagattgct gtttgatcag gtcctttcca ccacatctct
    32641 gagtattggg aagaatagtt ttctgcttcc aaaacttgat tatcaaagcc aattggtttt
    32701 acaccacggc caaacgctgc tgctgaaata ccagcttgtt tcattaattg aggcgtctgt
    32761 cccatatttc caaatgtatc gggaaaatag cctaacatga caggggttcc ccatttacgg
    32821 ctttcttcca tgccaatcag catatttctc acgttcgatt ccgagctgat aagaaaatca
    32881 tcttgcaaga tataaaatgg tccaattctc aattttcctt cattgattgc ttgttggatt
    32941 gcctgtcttt tttctggcct tacttgaaga tagtcatcta aaataatcgt ttgcccatcc
    33001 aaatgaaaac tgttgaagga cggatcaact tcaaatagct ctagtagatc gtctatcaat
    33061 tcaatcagtc gcatgtgatg ctgttcgtaa gccatgtacc attcacgatc ccagtggcta
    33121 tgagaaatga tatatacttt ctttttcatt tacgatacct cctacttttc tactctaata
    33181 tcaaagtaat ccatgactaa ttcgcaaaac atcatatttg cccaagaaaa ccattctctc
    33241 gtataatttt caggattgtt cacatcgaat ccttcgtgca taaggtgtgt tcctgcgtca
    33301 gttgctacaa gtaaattcaa tatccgctct ttttcagatt tgtctgacgt tgtcattcct
    33361 tccatagcta atgcaatcgg ccaaacgtat ttttctggcg tatgggagga tccaattcct
    33421 tttgcgtact ttccttcata gaaatatgga ttttctttac tcaacaatgt tcttcttgtt
    33481 tgcagataat cttcctcttg ttcagaacaa tatcccaagt aaggtgcggc gatcagattg
    33541 ggcacattac tgtcatccat gactgtggcg tttcccattc catctacttc atatgcataa
    33601 atcgtttgcc ctttttgatt aaccgttcgt ccgtgattct gaatcccctc ttggatctct
    33661 ttcttcaatt tctgtgtttg ctgtacaatt tctttatcat cgagtatcgt ttgaaaaatt
    33721 tcttccacat aacctaacac gaccaccgca aacatattgg atggtaccag atatccataa
    33781 cggcaagtat catcgctagg acggaaacca gaccatgtca ttcctgtaaa agctgttttt
    33841 gcacctcgcc cgtcattgat cagcgtatct tcttttcttg tcgtgtctcg tacaaatgta
    33901 taaggagagt gttcatgatc ttgctctgtt ttgaacacgt ccaagatttt tttgatacct
    33961 tcaataaaag attgattgaa ttgatcagtt tttcctgtat ttttgtaaag aagataggcc
    34021 aactgtattg ggtagcataa agaatcaatt tcatattttc tttcccagat ccaatcgttc
    34081 atttttgtat gatctgtttg gtggccagca ccgttagctt cttcgttgaa ggcattcgca
    34141 tatggatcga tattgatata aaaaaattgc tttttgacta acccgctgat catgtcagct
    34201 aattcctcat cttcttttgc aatcactaaa taaggacgaa cttgtgcagt agagtctctt
    34261 aaccacatcg ctggaatgtc tcccgtcaat aaaaaggtgg ttccgtcatt ttgctttttg
    34321 actgttgtca acagagtatt ggcaaatgct gctgtaaaat tctttgccca gtccgcatgg
    34381 atatccccac atttttgtgt gatatcttcc ataaatttct gcaccgattt tggtatatct
    34441 ttataagcca ctaataaaac cttctttcta tttgatatat ctttaaagat tatacttcat
    34501 acagccaatt atgtctacgc tttcaaaaaa tatcttttta tttacttttt catccattat
    34561 ggtattattt gaatatgata tatcattaag aggtgtttat atgaatcaac cattgtacca
    34621 gacaatattt aatgatttgc aaacggctat tttaactggt gaattacctg tagacagcca
    34681 actaccaact gaaaaagaac tttcacgtac ctataaagta agcagaatca cttccaaacg
    34741 tgcattgact gaattagagc aattaggact tatttatcgc gttcgcggga aaggcagttt
    34801 cgttaaatcc gcttctccgg aacagattct tgcttctcct acaaaaacac gacggattct
    34861 gtttcttctt ccatatgtcg ctgatttgtc tgttggtgat tttagcaagg gattgaaccc
    34921 tgtgatgcaa gataaacaga ttgatgtcat gatgacgacg cttgactttc tacaaaataa
    34981 aacagcaaaa gagctaatca atgaatttga cggattgatt tattatgctc ttgatacaga
    35041 acaacatctt gacttacttt ttgagctttc attgaaagaa ttccctgtcg tcatattaga
    35101 taaaaaaatc tatgagttac cttttcctac cgttctttcc gataatttcc aaggcggtgc
    35161 gcttgctgcc tcttatttga tcgaacaggg acattcaaaa atcgcttatt tgtttggtac
    35221 gcagactcat ccacaatccg ttcgacagcg ttaccttggt taccttgaag cattggacaa
    35281 agctggtttg actttccaca cttcactgga tgacaaaagc gcgacattag atgaattgct
    35341 ttcttatatc actaagtatg aagtaactgc ttttgtttgt gaaaacgatg ttgtcgctat
    35401 ccagaccatg aatctcttga gacagcatgg ttatcatatc cctgaagatt tttcaattat
    35461 cggatttgat gatatccaag cagcagctct tgttgacccg ccactgacaa ccatcgcaca
    35521 agattttgaa cagttaggta agcttgcggg agatgctttg gtcacttggt tagagacaaa
    35581 agatcgacca tccgatagta aatttccagt ttcattcatc aaaagacaat caacaaagga
    35641 gaaataaaat gaatatcggt caaatagata ctagacatgc aacagcgaat cagccaaatt
    35701 tctctaatgg gaactgccag ccttatactg gcgttccttt tgggatgaat tattttgcac
    35761 cgcaaactac ggatcaaaac ggcagctggt ggtttcatcc tgatgaccgc gtttttcaag
    35821 gctaccgtct tacccatcaa ccaagtcctt ggatgggtga tttcagtcat atgctgttta
    35881 cgccgatcaa tggaaaacta caggagaaca cccttttcca tgctcaaagt tcttaccgcc
    35941 cagaagaaag tatattttgc ccgactcatc tgtcgatctg tcaattacgt tatggcatct
    36001 gttctacatt gatacctagc atgtatggcg gaatcttatc tattgattac agcaaaaatg
    36061 acagcggttt acttttatcc tttccgggac gccatcagct ctttgttata gatccttaca
    36121 cagtggaagg aaaaatcaca cattttgctg gttcagaaga tcctgatttt tctttttatt
    36181 ttatattgag atttgaatat cctttaacaa ttgatgagtt agctgtttcc aacaaaaata
    36241 gcgactcgat cccgttgtat tttgaacaga caaaaaaaca aactatccga tttgggacat
    36301 cttttatcag ctcggaacaa gcagcttata acctggaact agaacaaaac caaagcaaag
    36361 agacttacct tgctcacagt caagaacaat ggcagtccta ttttgatcgt atacagctca
    36421 cacaccatga tcaacaacaa ttgcggacgt tttatcataa tttatatcgg gtgtttttat
    36481 ttccacaaac attttatgaa attgatccta gtgggcaaaa aatccactat gatacgacta
    36541 gtcgatctat aaaacctggt gttctctata caaataatgg gttttgggat acgtataaaa
    36601 cggtttatcc tctttattct ttgattgctg tagaaaaata cgaagaaatg ctggaaggtt
    36661 ttttgaacag ctatagagaa agtggctatt tgccaaaatg gctgtctcct gatgaacgtg
    36721 gacttatgcc tgggacattg atcgatgcgg tcattgcaga tgctgctgta aaagatatcc
    36781 gacgagactt gatgcctgaa tttctaacgg caatggaaaa aggtgctacg gttcaaagca
    36841 aacatccaaa ttatggacgc caaggaacag aagattactt gacatatggc tatgttccta
    36901 atcactacca cgaatcagtc aatcacacat tagactatgc ttatagcgat ttttgtatta
    36961 gccaagttgc caaggtacta gaagaagagg aaatgacgaa gaaatatcag cagcaatcat
    37021 tgaactatca aaatatcttt gactatgagt caggatttat gcgttcaaaa gataaagatg
    37081 gcaagttcag agaaccattt tcagctaatc gttggggaca agattatgca gaaggaagtg
    37141 cttggcaaag cagctttgct gtttatcacg atttttcagg actgatcgga aaatatggtg
    37201 gcagtgagag atataaagaa aaactcattg agctgtgcaa tcagccccca acgtttgacc
    37261 caggaggata tggttttgag atccacgaaa tgagtgagat ggccgcgata gaatttggac
    37321 aattagctat ttcaaaccag ccaagttttc actatcctta cttgttcagt tatataggac
    37381 aacctgaaat ggcacagcca ctgcttaaac agttgatgac tcagaccttc gacgattctc
    37441 caatgggtta ccctggagat gaggacaatg gaagtatggc cgcttggtat atcttcaata
    37501 gtctgggatt ttatcctgtc acgcctggaa ccggtgaata tgttatcggt atgccattaa
    37561 ttgacaaggc tgttttgctt ttgtcaaatg gaaaggaatt gacgattgaa gcaacgccta
    37621 atcggccgca gcaacaattt atccataaaa tcaaacggat ggacaaagaa tatacgaaat
    37681 tatattttac ccatcaagat ttgctgaaag gcggcacgat ctcttaccaa ttagggatca
    37741 tccctcaccc taagaaatat caagcaaaag atttgccttt ttccttatca aactataatt
    37801 aacaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    37861 gatcctcata tttttcgttt ggttggtctt attaccgcag atgcaagctt ttgaacaccg
    37921 tttattgaga ggttgtgaaa tcagcgttct gacctgagta ttagagagac aaatggcaaa
    37981 atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc
    38041 gtttagtgga gaggtcgtga aaaagctg
//
LOCUS       DACBZN010000015        36073 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.015, whole genome shotgun sequence.
ACCESSION   DACBZN010000015 DACBZN010000000
VERSION     DACBZN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.015"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            232..1500
                     /locus_tag="IVD64_000786"
     CDS             232..1500
                     /locus_tag="IVD64_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAP6148058.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="IVD64_000787"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="IVD64_000787"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP6148059.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="IVD64_000788"
     CDS             3076..3351
                     /locus_tag="IVD64_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP6148060.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="IVD64_000789"
     CDS             3357..4124
                     /locus_tag="IVD64_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP6148061.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="IVD64_000790"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="IVD64_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP6148062.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="IVD64_000791"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="IVD64_000791"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP6148063.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="IVD64_000792"
     CDS             8268..8570
                     /locus_tag="IVD64_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP6148064.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="IVD64_000793"
     CDS             8573..9736
                     /locus_tag="IVD64_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6148065.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="IVD64_000794"
     CDS             9762..13190
                     /locus_tag="IVD64_000794"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP6148066.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="IVD64_000795"
     CDS             13211..14335
                     /locus_tag="IVD64_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148067.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14325..14762
                     /locus_tag="IVD64_000796"
     CDS             14325..14762
                     /locus_tag="IVD64_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6148068.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="IVD64_000797"
     CDS             14746..15084
                     /locus_tag="IVD64_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP6148069.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="IVD64_000798"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="IVD64_000798"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP6148070.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="IVD64_000799"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="IVD64_000799"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP6148071.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="IVD64_000800"
     CDS             16241..17284
                     /locus_tag="IVD64_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6148072.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17443..18171
                     /locus_tag="IVD64_000801"
     CDS             17443..18171
                     /locus_tag="IVD64_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP6148073.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18287..18532
                     /locus_tag="IVD64_000802"
     CDS             18287..18532
                     /locus_tag="IVD64_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6148074.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18529..18747
                     /locus_tag="IVD64_000803"
     CDS             18529..18747
                     /locus_tag="IVD64_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP6148075.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18883..19422
                     /locus_tag="IVD64_000804"
     CDS             18883..19422
                     /locus_tag="IVD64_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP6148076.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19415..21688
                     /locus_tag="IVD64_000805"
     CDS             19415..21688
                     /locus_tag="IVD64_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP6148077.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21726..22772
                     /gene="holA"
                     /locus_tag="IVD64_000806"
     CDS             21726..22772
                     /gene="holA"
                     /locus_tag="IVD64_000806"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP6148078.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22861..23115)
                     /locus_tag="IVD64_000807"
     CDS             complement(22861..23115)
                     /locus_tag="IVD64_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP6148079.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23515..23784)
                     /locus_tag="IVD64_000808"
     CDS             complement(23515..23784)
                     /locus_tag="IVD64_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148080.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24254..24709)
                     /locus_tag="IVD64_000809"
     CDS             complement(24254..24709)
                     /locus_tag="IVD64_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP6148081.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24847..26775)
                     /locus_tag="IVD64_000810"
     CDS             complement(24847..26775)
                     /locus_tag="IVD64_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6148082.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26972..27805)
                     /locus_tag="IVD64_000811"
     CDS             complement(26972..27805)
                     /locus_tag="IVD64_000811"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP6148083.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27997..28701)
                     /locus_tag="IVD64_000812"
     CDS             complement(27997..28701)
                     /locus_tag="IVD64_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6148084.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28724..29350)
                     /locus_tag="IVD64_000813"
     CDS             complement(28724..29350)
                     /locus_tag="IVD64_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6148085.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29676..32051
                     /locus_tag="IVD64_000814"
     CDS             29676..32051
                     /locus_tag="IVD64_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP6148086.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(32075..32566)
                     /locus_tag="IVD64_000815"
     CDS             complement(32075..32566)
                     /locus_tag="IVD64_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP6148087.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32753..33673
                     /locus_tag="IVD64_000816"
     CDS             32753..33673
                     /locus_tag="IVD64_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6148088.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33827..34171
                     /locus_tag="IVD64_000817"
     CDS             33827..34171
                     /locus_tag="IVD64_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP6148089.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34361..34861)
                     /locus_tag="IVD64_000818"
     CDS             complement(34361..34861)
                     /locus_tag="IVD64_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6148090.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35389..35472
                     /locus_tag="IVD64_000819"
     tRNA            35389..35472
                     /locus_tag="IVD64_000819"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35423..35425,aa:Leu,seq:cag)
     gene            complement(35796..>36073)
                     /locus_tag="IVD64_000820"
     CDS             complement(35796..>36073)
                     /locus_tag="IVD64_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6148091.1"
                     /translation="VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11965 a         6295 c         7448 g        10365 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtagaaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    17341 gtcaaaaatc ttcacgataa aaatgaaaac aaaagaaaaa attgacgtaa ttagatgttg
    17401 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    17461 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    17521 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    17581 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    17641 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    17701 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    17761 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    17821 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    17881 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    17941 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    18001 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    18061 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    18121 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    18181 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    18241 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    18301 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    18361 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    18421 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    18481 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    18541 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    18601 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    18661 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    18721 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    18781 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    18841 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    18901 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    18961 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    19021 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    19081 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    19141 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    19201 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    19261 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    19321 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    19381 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    19441 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    19501 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    19561 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    19621 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    19681 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    19741 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    19801 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    19861 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    19921 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    19981 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt
    20041 atatattaat tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat
    20101 ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta
    20161 tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat
    20221 tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt
    20281 gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc
    20341 caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct
    20401 ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt
    20461 tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc
    20521 gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat
    20581 tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg
    20641 ctggctgccg gaaactttac ttttgttgat agaagaggga ttaatcttat ttgagcgaat
    20701 aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg
    20761 gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca
    20821 cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc
    20881 ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt
    20941 tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg
    21001 ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca
    21061 ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga
    21121 attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg
    21181 acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat
    21241 tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt
    21301 tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag
    21361 gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta
    21421 tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa
    21481 cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa
    21541 tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac
    21601 atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca
    21661 tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta ggataatttt
    21721 tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac
    21781 ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag
    21841 aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca
    21901 tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag
    21961 atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg
    22021 gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg
    22081 tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat
    22141 tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat
    22201 atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat
    22261 tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc
    22321 tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa
    22381 cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag
    22441 ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga
    22501 ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc
    22561 agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccatgc
    22621 aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga
    22681 tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt
    22741 tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg
    22801 aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt
    22861 ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat
    22921 tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac
    22981 gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct
    23041 tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga
    23101 ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat
    23161 aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt
    23221 ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct
    23281 gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt
    23341 atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa
    23401 agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt
    23461 ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc
    23521 tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt tttttatgac
    23581 caactggttc cgacggttcc atttgccact tacttccagc acatatttat ttaggggcaa
    23641 acgtaagata tattttgcca aatagcgatc tttaagcagg ccattgatca cacggtcctc
    23701 aagctgcaag ttgatcccag tcagcatcat tggctgttct tgtaaaacgt acgtatcaat
    23761 taaaagacct tcaaaagttt ccacaagcgt cttcctttct tttcaaagta acctgaaaac
    23821 aggcactaat tcttccattt tgatattcgt tgaagtggat gcgatcattc tttcttttgt
    23881 ccactgtaat ctagtataac aaaaaagagc agaagagcca aaaagaaaaa agagcatcga
    23941 attctatagg aagtctcatt ggcacggcct aaattattac aaaacattga ttaaaaaaag
    24001 cccctgcatc gcaggagcat cccctatatc gttttaatag gtcaatcaaa aagcagtatc
    24061 aaacacattt tgtatcaaat caggaaataa tagataagct aagcccagaa tcaaaagtac
    24121 cgctagaaga aaccagccga ttgttcctat gaaatacttt aatttttcga acattgtatc
    24181 acctcgttgt cagtatatca cggatcagta aattgggggt gttaagaatc tcctaaaata
    24241 gacaaaattt gttctatttt cctaaagcaa atacttgtaa gatacagtaa gtgataccag
    24301 aagcaatgac cggtccggcg gcgattcctc ttaagaagac aacacctaaa atcgtgccga
    24361 aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa tcctacaccc ttggaagata
    24421 agacagccac tagcccaccg caaaaaacag ctacccagcc aataggggat ttaaaggctt
    24481 ctaatagatc acgtagaccg atttctcctg ttgcgatagg gattagaata gccgctgtga
    24541 tgatcgtcac cccaatattg attcctttgc cttgaagcca agggaaaaag gaacgggagg
    24601 ctggtatgag cttcataatc aaaacaaaag ctgccgctat catcagactt tgatttttag
    24661 ctaaaattgc tacaagtaaa ataattccta aaaaaagcca actttccatg aaatatattc
    24721 ctcactttct tcgaaacaat cataacatgt ttttagaaag ttacaaaagt aggaattcat
    24781 ccttcaagac ctattcataa gctgtttttt tcataaaata aacaaaatca taaaaaaaca
    24841 ctttgcttat ttgacagaag acagccgcgc tttgtatagt ggacaagact tacatttttt
    24901 tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc aagccattca gaataaccaa
    24961 tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt
    25021 agaagcaatc aacaagagaa taacaagaat cgaaaaaata atattttgtt tgatgatccg
    25081 ccgcattttt aaagatagcc gatgactgta gacaaattta tttaagtcgc ttttcattaa
    25141 gacgatgtct gcgatatcca tagcggcatc tgtcccttgc cccatagcga cacctaaaga
    25201 agcggctgct aaagcaggcg catcattgac tccatcacca accatcgcat ttacttggta
    25261 aagttcattc tcttttttga ctagttgtgt tttttcttcc ggagtacagc ttgcatagta
    25321 atgttcaatc cctacttgtt cagcaatggc ctttgctgtg ccagaatggt cgccggttat
    25381 catacttgta tggatgtttt gggatttaaa gtaggaaatt acttgttggg tatttgcttt
    25441 tggaatatct agcagaccta aaacagcaat taactgctga tctttagaaa gataaatcac
    25501 ggttttgcct tgattttcca aacgttcgat catttccgca atctctttgc tgattatcat
    25561 cgtctcagga tcatatgcgt gcttgccaac tttccattgc gcacctaaat aggtagtttg
    25621 taatccaaaa ccagtaattt cttctattgg taagtattgg atttcttcag gtattgtgca
    25681 atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt ccatagcaac
    25741 aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta caactggttt
    25801 tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct tttaattcag aaagcttttc
    25861 taaatggata ccgcctttga ttaaaatccc attttttgca gaatttgaaa gagcagctag
    25921 agaagctggt gtagcagatg cgacaagcgc acaaggagaa gccacaacta ataataccat
    25981 tcctcgataa aagctttcat cccaactcca acctaggaaa aagtggggga gaaggatcat
    26041 tagaggaaca gcaagcaaaa tcaattttac ataattattt tcgattcgtt cgataaatga
    26101 agctgtttga gttggcgtat tctgcgcttc ttctactaat tggataatct tagcaaagac
    26161 agtttcggaa ctcgtttttg ttactcgaat cgtgatggga tttccttgat tcagcgttcc
    26221 tgcatacatc tcatcgccta aatatttttc tacaggcaca gattcaccgc ttaatgcaga
    26281 ctcatcgatc gttgcctgtg tgctttctaa aaagccatct atcggaacag ctgctccttt
    26341 agcgacaaat accaagttat ccacctttag ttgagaaaca tctacttctt gcatttgtcc
    26401 attttctttt aataattggg ctttttgtgg ttgaagattc atcaaagcag taatttcttt
    26461 ttgactttta ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa
    26521 cattgctcct tcaaagtaat taccgatcaa acaggcacca atagcagcta aagccatcaa
    26581 taaatccaca ttcagtgtcc gatcattcca caattctttc aagccttccg atgtttgttt
    26641 atatccaccg atgataatgg cactgatata aaaaagtggg gtgatggtaa gagagaatcg
    26701 gtcgaagata aaaccgaaga tcataaataa aaggcagccg attgttgctg caaaagcttt
    26761 tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca taataaaaat
    26821 gtaattgata ataataacag ctaaatgaga acgataatgg ttatcatttg gtgattaagc
    26881 ttgatgttgt ttttattatt tataatgatt attattaaag aaaataaaaa accagaacat
    26941 ttctcaaata agagaagtgt tctggttttt tctacattgc aataaaccaa taaccaatca
    27001 aaatgatacc aaggataata cgataccagc caaaagcggt aaagtcgttt ttcttcaaat
    27061 agcttagtaa gaatttgata gctaagacgg aaaccacaaa agatacgaat gttcctacaa
    27121 gaagaacaac agtttcttcc attttgaatg aattcccttc agctaaatat ttgacgatct
    27181 tcaagccgct tgcgccaaac atgacaggaa tccctaagaa aaatgaaaac tctgtagcga
    27241 caaaacgcga agcaccaatc aagatagatc ctaggatcgt tgcgccagaa cgggatgttc
    27301 cgggaacaag tgccaagact tggaataacc caacaagaag agctgcttgg tacgtaaagt
    27361 cattcagaga agtccaacga ggtgttttgt ttttgttgcg tttttcgaca ataataaagg
    27421 caataccgta aacgatcaaa acaatggcaa ctggtaagaa cttgtggaaa tttgcttcta
    27481 accaatcatc caaaggcaaa ccaatgatga tggctggtat gcaagcaacg atcactttaa
    27541 accaaagtgt ccacgtatct tttttttcta ccgttgattt acttggagaa aatgggttta
    27601 acttgtgaaa ataaagaacg acgacagcca agatcgcccc taattggata acgacattga
    27661 acatcgacat aaattgtgaa ctggcatcca gtttgatgaa ttcatctgct aggatcaaat
    27721 gaccagtact actgatgggc agccattccg ttattccttc aataataccg agaatcactg
    27781 ctttgataat atttgcaaaa aacataaatt ccttccttcc caattaagat acatcttata
    27841 gtataccgtt attttttgag aaacccaatc tgttttttaa tattaagaaa aacttgagaa
    27901 ataaagcaga taagtacacg aagagaaaaa agaagacaaa gccctcgttc agactttgcc
    27961 ttttatgatt ctctggtaaa aattaaaata tccccattat agaaacgagt cgcgactatg
    28021 tgttttcccg ttatcgatat aagacgtatc attgatgccg tggatagaaa atcgaccgtt
    28081 tgcataagtg acctttgtta cgctgccatt taaaagccca gcacgaacag gctgtttagg
    28141 atcgatcaga ttaagcaaaa aggcgatcgt caaaccatga gatacgacta aaacatttcc
    28201 accaccattt ttttcgcagt gatgggcgat atcttcaaat cctgtccata cacggtctcg
    28261 caatgtttca taagtttccg cccacccagc ggtatctaag ttgtatacag cattagcaat
    28321 ttgttcaaaa gaaacttctg tggtaaacat ttcatcatat gttttaaaat tcaatacacg
    28381 agggatcaca ccccacattt ccatgtcata tccaccttca agagacccga aacaccattc
    28441 tctaatcctt ttttcaataa aataaggaat ttcttttccc ttagaatgtt ctcctagaat
    28501 aatacgggca gtttcgattg cacgaccact gtcactagaa taagcaaatg aaaagtcgac
    28561 attttttaac ccgcgaccta aataatgaat gccttcttgt ccttgctttg tgaggggtgt
    28621 atcacaccag ccttgtactc gttcaattgt attgaacatc gtttttccgt gtcgtacgat
    28681 ataaagtgta gtttctgtca ttttattctc ctcctaagtc gttttaacga ttgaaaaaag
    28741 gatttgtcct tttttcctgt tcaatcgtcg ttgcatcgcc atgtcctgga tagactgcaa
    28801 attcatcagg taatacaaac agctgggtcc gaatactatg aagtaattgt tctaaattcc
    28861 ctgtatacag atcggttcta ccgatacttc ctctaaacag ggcgtcgcca gaaacgacaa
    28921 aatcatcaaa aataaagctg acgcttccta tagaatgccc tggggtaggg acgacgcgaa
    28981 aagacatgtt tcctaaagta taagttttca tttcaaattc atattctgca ggcgagacaa
    29041 taacatcagc aatatcatca tgtctaccga gtccagaaag attcaaaatc ggatcaccta
    29101 gccatttttg ctctagtggg ctgacataaa ctgggatttg ataatacgcg cgtaaaggtt
    29161 ccactgcacc gatatgatcg tagtgagtat gggtaagcaa aatcgctacc ggctgttggt
    29221 ttgttttttt gatttcttgt ttgattcgtt ccgcatcttc tccagggtca atgatcaaaa
    29281 gtgcttcttc gttgtagacc aaataacagt tttcttcaat cattcctgtt tttaatcttt
    29341 cgatatgcat gtgcattcct ccttgatagc caaaacttct ttcatccatt agtataacgc
    29401 attcattgct aggaaacaaa aacaggtact tatttctctt tacttatctg aattatcaga
    29461 ctatatgaaa agtatttgga tattctaaag agaaggaaag taagttttca tagcatttta
    29521 atatgtcctt gttacattag attcatccaa aaatccaaat tctatatcct atatccctgc
    29581 ctaatcagca gggtgttttt ttatgtttgg aaagagagct ctttttgcta aactaggttt
    29641 tttcaggtaa tcttagagag aaagaggtgg tggaaatgat caaacagtca aaaatatctg
    29701 tgcggcactt agtagaattt attttgcgac gtggaagtat cgacaatcgt aaaaaaagta
    29761 accatactgc attagaagga gcgagaatcc accgcaagct ccaaaaagat gcgggagaaa
    29821 actatgaaaa agaagtcttt ttaaaaacaa cagttcaatt agatgaatat tacctgatcg
    29881 ttgaaggccg tgcagacggt attttcaaaa gagacggtat ttattatata gatgaaatca
    29941 aaacctccga accacgattt gaagatttag aaccagaaca agtggaactt ttctttcatc
    30001 aagcaagggt gtatgcctat atctatagtc aagaacatga tttacaagaa ataaatctgc
    30061 aattgactta cttccagacg gcagaagaag tgatcacgag aaaggttgaa catcagacaa
    30121 gagagcagtt agaaactttt tttaaaaagc tgactgaaga ttataaagaa tggcttgttt
    30181 ttcaagaaaa ctggcgcact gtacgcaatg cctctttgat ggctttgaaa ttcccccatg
    30241 atgagtatag gaaaggtcag cgtgaactag cggtagctgc ttataaaacg atccgtacga
    30301 aacagaaatt gtttgtagaa gcacctaccg gaacaggaaa aacaatctct acgttattcc
    30361 ccgctttgaa agctgtaggg gaagaagagg gagaaaagat cttttatcta acggctaaaa
    30421 caataactag acaagtagct gaagatgcta tgacagcatt aaaagataca ggagctgaag
    30481 taaaaagtgt cactttgacg gcgaaagaca agatctgctt tttagatgag acgacctgta
    30541 atccagatca gtgcccatat gccaatggtt attacaatcg gattaatgaa ggtttatggg
    30601 atcttttgaa tcatgagaat cagatcactc gtgaagtcat cgaaacttat gcaagaaagc
    30661 atacactttg tcctttcgaa ctatcattag atgtcagtat ctggtgtgac gtaatcattg
    30721 gggattacaa ttatttattt gatccaactg tttatttaag acgttttttt gaagatgaaa
    30781 aaaacgagga ctatctgttt ttgattgacg aagcacataa tttggtgaat cgttcccgag
    30841 agatgtattc ggcagaacta tcttatgaaa aaacgaaacg aagtaaggaa gctataccaa
    30901 aggaattcaa aaaacttcat cgccgtttca acaaactatt aaaagaattt gattcgatcc
    30961 gagaaatagc aaaagaagat cattgggatt atcatcatca aaaagcaccg gctgaatctt
    31021 tagtaaaggc aggttatcaa ttaagcgaaa aaattaaaga atggctggca gaatttcctg
    31081 agcacccatc acaagaacaa ttgcttcctt attattttga tttgcttcat tttttgaagg
    31141 tcagtgaatt ttatgatgat cactacgaaa caacagtcga aaaaacgtat cgcgatctga
    31201 tagtaaaaga gttttgtatt gatccaagct tgtttcttga gcagtcgttg gataaaggaa
    31261 aaagcagtat tcttttttct gctagttttt caccactctc ttattatcag gaaacactgg
    31321 ggggacaaaa aagtctggct tacaaactgc cgagtccttt cccagaagga aaccagcaag
    31381 ttttcatcgc aaattaccta gagacgactt atcgaaaaag ggaacacagt ctggcaaaat
    31441 tagtagagac gattcatgct tttgctgatg gaaaggtcgg gaattatttc gttttctttc
    31501 cttcttatca atatttagat cttgcggtgg aagcattcag acagcaatat cccgacagca
    31561 atgtgctaat ccaagaaacg gacatgaatg aggaagaacg cgaacgattt ttaacaaaat
    31621 ttcaaacaaa tcccgctgaa acattgattg gattttgcgt acttggcggg attttttcag
    31681 aaggaatcga tttgagaggg gaccgattga tcggcagtat gatcgtaggt gtaggtttgc
    31741 cgcagatgaa tcatgagcag gaactgatca aatcatatta tgatgagaag gaacaccttg
    31801 gatttgctta cgcttatcag cttcctggaa tgaacaaagt cttgcaagct ggtggtcgag
    31861 tgattcgtgg catggcggat caaggaatcg ttgtattggc agatcaacga ttttctagtt
    31921 tttcttatcg acgtttattc ccacaacatt ggcaaacagc aagagaagta cgaagtgttg
    31981 gggaattgga agaaggaata cagcgttttt ggttatcaaa agaagcagta aatcaaaaaa
    32041 acgaggaatg acagacgtca gcctcgtttt ttgattaacg atagttagtg aattgcaact
    32101 cgattggtaa gtcaagttta cgtaataaag caatcacagc ttgtagatca tcacggttct
    32161 tacctgtcac tctaatctga tcttcttgga tttgtgcttt tactttcagt ttttcatttt
    32221 tgatagctgt agtgattttc ttcgcgtttt cacgatcgat tccgttgacc aaatcggcag
    32281 tttgtcgagc tttgccacct agagcgtgtt ctgattcgga aaaatggata tttttgattg
    32341 gcacatttct tttgatcagc ttaccaaata atacatcttt gatctgttcg attttgaaat
    32401 catcatcgct caatacaact agattgctgt tctctaattt gatttctact gtagaatcct
    32461 tgaagtcaaa gcgattggac aattcttttg ttgtttgctg aatcgcattt tttacttctt
    32521 ctgtattgac ttctgataca atgtcgaagc tagcatcttt tgccatagga accctcctga
    32581 tatacatata aatatttacc atacatctat cttatcatag gattagggca gattcttacc
    32641 tgtttgctaa aattcttctc atttgagttc gaagtatttt attttaatag ataaactgtt
    32701 attcttacat ttggaatacc aaagtagggg agaggtgctt gtgagtaaaa ttatgactgc
    32761 gtatcataaa aatgaaagat taaagaaaat agtggttatt ttgataaccg gattgaccgc
    32821 tgcggtcgct ttaaattatt ttttgattcc agccaatgtc ttttctgcag ggatgaatgg
    32881 gatcgctcaa atcatttcct cgcttttata tgaatggttt catatcaaga tagacacagg
    32941 aacatttatt tttcttttga atatccctgt gtttgtatta gggtttctca aagtaggaaa
    33001 acgagcaacg atccttagtt ttgtgaatgt tgtatgtgta tcggtgatga cgatgctgct
    33061 tccagtcgga cagatcactg ataatatttt gatgaatgcc ctagtaggcg gtgttttatt
    33121 aggtcttggc gtaggattat ctttaaaaat gggctttaca actggtggga tggatatcat
    33181 ctctcttgta ctttcacaaa caactggaaa aacagtcgga aactacatgc tgattctaaa
    33241 tggtatcatt gtagtcatcg caggcttctt ctttaactgg gaaagtgcct tgtatacgat
    33301 tatttccatt tatgcaatga ctcaagtaat tgatgccatc cacacgagtc atcaaaaagt
    33361 aacagcaatg atcgttaccg tcaagccaga agaggtaaca gaagaactag caaaacggat
    33421 ggtacgtgga atgacattac tgccgtctgt cggtggctat tcaaaaaaac aagggaacat
    33481 gatcatgatg gtcatcaccc gctacgaaat gtatgatctt gatcagatcg ttcatgaaat
    33541 cgacgacgat gcatttatca atattttacc aactcattca gtatttggcc ggtttgcaaa
    33601 tgaaaacgaa caaaaaatgt atcgaacaac tggcgtattg ccagaaataa aaaatcgttc
    33661 acaaaaaaaa tgaggaatca ggtttatttt agaataaatt taatctatta catctaataa
    33721 gaacattttt ataaatttaa acgagaatct tacttctaaa ttctcattta caagcagcta
    33781 aaaaaagagt atgataaata aaaatcgtgt taaggagtgt cgtacgatga gcgaagcaaa
    33841 ccaagaacgg tctggacaag aaatagaact aatcaaagaa cgtattcttg ctgccttgga
    33901 aatggtgatt gatcctgaat taggaatcga catcgtcaat ttaggcttga tttatgatat
    33961 agaattcaat cctgaaaatg gagaaacagt cattaagatg actctaacaa ctatgggctg
    34021 tccattagca gatattttaa cagaatctat ccatgacgca ttaaaagaag tgccagaagt
    34081 aacgaaagca gaagtcaaac tagtctggta tccagcatgg acgacagata agatgagccg
    34141 ttatgcacgg attgcgttgg ggatacgata gaaatcttgg ctgactagct atgagaagca
    34201 aataggaaaa acaaaaaagc cttttatcct ttctagaaga aaagagataa aaggtttctt
    34261 tgtttataaa attttgtctg ctatattata agttaattaa ataaatgtgt tctattgata
    34321 aaggacattc aaatcccttt ccttttggaa tccttctttt ttacatcagt ttgattttcg
    34381 tatagtattc tttctcatct tcacgataaa tcgcagcccc atttttttgt tgtgctttaa
    34441 gactgccaag atttttttta tttacagaca aaagtgcctc atcaatgccg attttttttg
    34501 cctcttgtag tactaatgct aatccttgtg tggcatatcc ttttttacga taattagggg
    34561 agatgccgta accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt ttatggcgca
    34621 aattaaaaat tcctacatac tcatgttcat cactttctaa aataaagacg gttgcgggaa
    34681 cgtaactctc aggaagatcg attcctaaag acgttttttc gcattttttt acataatcta
    34741 aaaattcagc aaatgtatag ccataagcat tgttgataaa tccactctca tttggtccaa
    34801 agctagtttg tagctgataa gctttcttta aatctgcttc ccatagtttg atcagtttca
    34861 tcgtattcac ctctttaagt aaccttattc atttgttttc tactatagca ctattgttgt
    34921 aaatagccat gcctattttt atttattgtg tcatatcgta ttaagaatca tcgaacaata
    34981 caaatatgaa ttacaataaa aaaattattt gaagttcgtt cattcgttac ttatgatctc
    35041 tagaagaaag aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac
    35101 agaagaaata atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa
    35161 agaaatgaag aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa
    35221 tcgatcatat ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg
    35281 gatgaattat ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa
    35341 tatgttcttg acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg
    35401 gaattggcag acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc
    35461 ttcttagccg cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag
    35521 aaaataacat attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa
    35581 ataaatcaaa aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa
    35641 atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag
    35701 actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat
    35761 attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag
    35821 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    35881 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacggccg
    35941 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    36001 tttgcagagc ttgtacttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    36061 gtttcagcaa cag
//
LOCUS       DACBZN010000016        35019 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.016, whole genome shotgun sequence.
ACCESSION   DACBZN010000016 DACBZN010000000
VERSION     DACBZN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.016"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(56..2653)
                     /gene="adhE"
                     /locus_tag="IVD64_000821"
                     /gene_synonym="adhC"
     CDS             complement(56..2653)
                     /gene="adhE"
                     /locus_tag="IVD64_000821"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP6148092.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="IVD64_000822"
     CDS             complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="IVD64_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP6148093.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="IVD64_000823"
     CDS             complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="IVD64_000823"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP6148094.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4798..5496
                     /locus_tag="IVD64_000824"
     CDS             4798..5496
                     /locus_tag="IVD64_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6148095.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5664..8303)
                     /locus_tag="IVD64_000825"
     CDS             complement(5664..8303)
                     /locus_tag="IVD64_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6148096.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8922..9953)
                     /gene="queA"
                     /locus_tag="IVD64_000826"
     CDS             complement(8922..9953)
                     /gene="queA"
                     /locus_tag="IVD64_000826"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP6148097.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10146..11321
                     /locus_tag="IVD64_000827"
     CDS             10146..11321
                     /locus_tag="IVD64_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6148098.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11325..11966
                     /locus_tag="IVD64_000828"
     CDS             11325..11966
                     /locus_tag="IVD64_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148099.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            11963..12880
                     /locus_tag="IVD64_000829"
     CDS             11963..12880
                     /locus_tag="IVD64_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6148100.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            12877..13548
                     /locus_tag="IVD64_000830"
     CDS             12877..13548
                     /locus_tag="IVD64_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148101.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(14089..14577)
                     /locus_tag="IVD64_000831"
     CDS             complement(14089..14577)
                     /locus_tag="IVD64_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP6148102.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(14778..15044)
                     /locus_tag="IVD64_000832"
     CDS             complement(14778..15044)
                     /locus_tag="IVD64_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6148103.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(15044..15307)
                     /locus_tag="IVD64_000833"
     CDS             complement(15044..15307)
                     /locus_tag="IVD64_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6148104.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(15300..15572)
                     /locus_tag="IVD64_000834"
     CDS             complement(15300..15572)
                     /locus_tag="IVD64_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148105.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            15783..15968
                     /locus_tag="IVD64_000835"
     CDS             15783..15968
                     /locus_tag="IVD64_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6148106.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(16110..17933)
                     /locus_tag="IVD64_000836"
     CDS             complement(16110..17933)
                     /locus_tag="IVD64_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP6148107.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(18531..21236)
                     /locus_tag="IVD64_000837"
     CDS             complement(18531..21236)
                     /locus_tag="IVD64_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP6148108.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(21542..22489)
                     /locus_tag="IVD64_000838"
     CDS             complement(21542..22489)
                     /locus_tag="IVD64_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP6148109.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            22679..22948
                     /gene="rpsN"
                     /locus_tag="IVD64_000839"
     CDS             22679..22948
                     /gene="rpsN"
                     /locus_tag="IVD64_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6148110.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(23124..23486)
                     /locus_tag="IVD64_000840"
     CDS             complement(23124..23486)
                     /locus_tag="IVD64_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6148111.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(23497..24618)
                     /locus_tag="IVD64_000841"
     CDS             complement(23497..24618)
                     /locus_tag="IVD64_000841"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP6148112.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(24634..24984)
                     /locus_tag="IVD64_000842"
     CDS             complement(24634..24984)
                     /locus_tag="IVD64_000842"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP6148113.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(25114..26625)
                     /locus_tag="IVD64_000843"
     CDS             complement(25114..26625)
                     /locus_tag="IVD64_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6148114.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(26947..28299)
                     /locus_tag="IVD64_000844"
     CDS             complement(26947..28299)
                     /locus_tag="IVD64_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP6148115.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(28452..29528)
                     /locus_tag="IVD64_000845"
     CDS             complement(28452..29528)
                     /locus_tag="IVD64_000845"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP6148116.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            29724..31469
                     /locus_tag="IVD64_000846"
     CDS             29724..31469
                     /locus_tag="IVD64_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP6148117.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(31749..32186)
                     /locus_tag="IVD64_000847"
     CDS             complement(31749..32186)
                     /locus_tag="IVD64_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP6148118.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(32170..33543)
                     /locus_tag="IVD64_000848"
     CDS             complement(32170..33543)
                     /locus_tag="IVD64_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148119.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(33865..34353)
                     /locus_tag="IVD64_000849"
     CDS             complement(33865..34353)
                     /locus_tag="IVD64_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6148120.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(34375..34977)
                     /locus_tag="IVD64_000850"
     CDS             complement(34375..34977)
                     /locus_tag="IVD64_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6148121.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9919 a         7476 c         6466 g        11158 t
ORIGIN      
        1 ttttccaagt tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc
       61 cgttgtaagc atcaatgatg atctgtttca attcagagat caatggttct ttcggattag
      121 ctgtcgtaca ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg
      181 tttcttgtgt gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt
      241 cataaacagc cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta
      301 agaattttgc gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca
      361 tcgcatgttt ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat
      421 ttgtccgtcc atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa
      481 tacctaggaa tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt
      541 cacgtgattc catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga
      601 ttgcttgtag gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga
      661 ttgcatgtgt taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga
      721 cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt
      781 tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg
      841 atgttgtcgg gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt
      901 tacggatatc caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga
      961 acatccacat tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat
     1021 ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg
     1081 gttcaacatc tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac
     1141 gaactgtatc gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt
     1201 caacgttttc cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg
     1261 gtaatttgaa ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt
     1321 ttacagcaga gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca
     1381 atgacggaat catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca
     1441 ctaaaatacg acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg
     1501 aatggataac tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa
     1561 acgcatggtc cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg
     1621 atagcggata atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg
     1681 gtacactgat tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat
     1741 tgaccgcata ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt
     1801 ttacgatata aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat
     1861 ccacgatcac agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga
     1921 tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac
     1981 ctggacctac acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc
     2041 cacctgtcgc taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga
     2101 tcgaaggatg ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat
     2161 cacgtaccac tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa
     2221 cgattggatt tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat
     2281 tcgttgttgg agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt
     2341 tttggacatc ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat
     2401 attcggacgc atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct
     2461 ctacagccat cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca
     2521 cgatatggtc tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt
     2581 tttctgccaa atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta
     2641 ttactttagc catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg
     2701 tatatcatac aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta
     2761 tttttaaata taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat
     2821 ttattattat ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct
     2881 tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt
     2941 taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt
     3001 gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt
     3061 gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca
     3121 aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca
     3181 actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag
     3241 ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat
     3301 agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg
     3361 tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt
     3421 taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa
     3481 aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt
     3541 agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca
     3601 catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat
     3661 ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct
     3721 tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg
     3781 tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa
     3841 cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat
     3901 ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga
     3961 tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat
     4021 ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt
     4081 tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc
     4141 atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac
     4201 atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata
     4261 tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc
     4321 ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa
     4381 tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt
     4441 gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt
     4501 ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag
     4561 cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc
     4621 caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt
     4681 taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca
     4741 gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg
     4801 caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa
     4861 gctgttttac taaatctaat ccctacattg attggtattg ctactattct tcttattatg
     4921 attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg
     4981 acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc
     5041 gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat
     5101 cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg
     5161 atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt
     5221 cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc
     5281 caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg
     5341 gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta
     5401 gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa
     5461 gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag
     5521 ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc
     5581 aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag
     5641 aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac
     5701 ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga
     5761 ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc
     5821 gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg
     5881 actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct
     5941 aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata
     6001 gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca
     6061 tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt
     6121 ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca
     6181 tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac
     6241 agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata
     6301 gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca
     6361 aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc
     6421 cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca
     6481 gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct
     6541 ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat
     6601 aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct
     6661 ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca
     6721 gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg
     6781 atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct
     6841 tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca
     6901 ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat
     6961 gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt
     7021 cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt
     7081 gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata
     7141 acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat
     7201 atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc
     7261 catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata
     7321 acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc
     7381 gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt
     7441 acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc
     7501 agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg
     7561 cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga
     7621 tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga
     7681 cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct
     7741 tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat
     7801 tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg
     7861 gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg
     7921 ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt
     7981 tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc
     8041 agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc
     8101 gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc
     8161 atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc
     8221 acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct
     8281 attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt
     8341 acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt
     8401 tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat
     8461 tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca
     8521 gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca
     8581 aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt
     8641 aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat
     8701 gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac
     8761 accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg
     8821 aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt
     8881 aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca
     8941 tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc
     9001 aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa
     9061 ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc
     9121 catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga
     9181 attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc
     9241 tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta
     9301 tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca
     9361 acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc
     9421 gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga
     9481 cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa
     9541 attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact
     9601 gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt
     9661 ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc
     9721 gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata
     9781 actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc
     9841 tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt
     9901 tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca
     9961 ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca
    10021 gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt
    10081 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
    10141 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
    10201 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
    10261 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
    10321 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
    10381 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
    10441 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
    10501 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
    10561 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
    10621 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
    10681 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
    10741 cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata
    10801 caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag
    10861 atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag
    10921 caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac
    10981 gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag
    11041 aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg
    11101 ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg
    11161 ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg
    11221 catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg
    11281 ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc
    11341 atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg
    11401 ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa
    11461 aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt
    11521 tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct
    11581 ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt
    11641 ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg
    11701 ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg
    11761 tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt
    11821 ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa
    11881 ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg
    11941 cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc
    12001 tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac
    12061 catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg
    12121 aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac
    12181 tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg
    12241 aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc
    12301 taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt
    12361 tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa
    12421 agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat
    12481 caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc
    12541 gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat
    12601 tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga
    12661 tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc
    12721 tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa
    12781 aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca
    12841 acgtttctta caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat
    12901 gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt
    12961 atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg
    13021 gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat
    13081 atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg
    13141 ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat
    13201 acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg
    13261 atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg
    13321 gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc
    13381 ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg
    13441 attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta
    13501 ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa
    13561 aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt
    13621 aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct
    13681 atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc
    13741 gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc
    13801 atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc
    13861 aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt
    13921 cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct
    13981 tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca
    14041 gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca
    14101 tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac
    14161 caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg
    14221 tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc
    14281 cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac
    14341 gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc
    14401 tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc
    14461 tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt
    14521 cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact
    14581 tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc
    14641 cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt
    14701 ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa
    14761 ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta
    14821 ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg
    14881 ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc
    14941 aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa
    15001 tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat
    15061 caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta
    15121 aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa
    15181 tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat
    15241 ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat
    15301 tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca
    15361 aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa
    15421 agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg
    15481 gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa
    15541 gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga
    15601 ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg
    15661 attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat
    15721 tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa
    15781 aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt
    15841 atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct
    15901 gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt
    15961 acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata
    16021 gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag
    16081 agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg
    16141 acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg
    16201 agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag
    16261 ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg attttcaatc
    16321 gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct
    16381 gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca
    16441 taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg
    16501 actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga
    16561 agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt
    16621 ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg
    16681 attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt
    16741 ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg
    16801 ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg
    16861 atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct
    16921 tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca
    16981 gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa
    17041 tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt
    17101 tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag
    17161 atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat
    17221 ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct
    17281 cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc
    17341 aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca
    17401 aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga
    17461 ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt
    17521 gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac
    17581 atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca
    17641 ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat
    17701 agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg
    17761 gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat
    17821 gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca
    17881 ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa
    17941 actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat
    18001 gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa
    18061 acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt
    18121 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    18181 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    18241 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    18301 gcttgcatct gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg
    18361 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    18421 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    18481 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    18541 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    18601 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    18661 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    18721 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    18781 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    18841 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    18901 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    18961 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    19021 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    19081 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    19141 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    19201 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    19261 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    19321 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    19381 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    19441 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    19501 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    19561 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    19621 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    19681 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    19741 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    19801 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    19861 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    19921 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    19981 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    20041 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    20101 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat
    20161 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    20221 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    20281 tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    20341 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg
    20401 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    20461 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc
    20521 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    20581 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    20641 tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    20701 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    20761 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    20821 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    20881 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    20941 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    21001 tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    21061 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    21121 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    21181 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    21241 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    21301 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    21361 tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg
    21421 aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca
    21481 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
    21541 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
    21601 gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg
    21661 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
    21721 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
    21781 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
    21841 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
    21901 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
    21961 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
    22021 acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
    22081 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
    22141 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
    22201 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
    22261 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
    22321 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
    22381 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
    22441 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
    22501 ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaaatg tcttttcgtt
    22561 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
    22621 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
    22681 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
    22741 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt
    22801 agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata
    22861 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
    22921 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
    22981 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc
    23041 tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag
    23101 gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca
    23161 ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt
    23221 tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt
    23281 tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt
    23341 tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga
    23401 ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca
    23461 aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat
    23521 ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc
    23581 ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg
    23641 aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca
    23701 gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata
    23761 accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc
    23821 gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc
    23881 tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc
    23941 tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt
    24001 gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc
    24061 tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa
    24121 accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc
    24181 aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg
    24241 cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc
    24301 tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt
    24361 caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt
    24421 tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc
    24481 atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc
    24541 aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg
    24601 gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa
    24661 agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt
    24721 cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc
    24781 gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa
    24841 ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat
    24901 tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc
    24961 atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc
    25021 attctaccaa attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt
    25081 ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa
    25141 tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct
    25201 ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt
    25261 tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc
    25321 tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag
    25381 tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt
    25441 atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc
    25501 tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt
    25561 ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc
    25621 ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat
    25681 atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt
    25741 cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg
    25801 ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc
    25861 caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac
    25921 atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg
    25981 atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat
    26041 gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg
    26101 tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa
    26161 catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg
    26221 gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa
    26281 gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt
    26341 gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc
    26401 gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc
    26461 aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa
    26521 tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac
    26581 tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata
    26641 tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt
    26701 attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc
    26761 accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct
    26821 ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt
    26881 tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac
    26941 agatttttat ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct
    27001 gccttttagc ataatactat ctgttgctgc aagattttgt ttgatagcat caatcaattt
    27061 attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc
    27121 ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat
    27181 atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc
    27241 taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc
    27301 catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt
    27361 tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg
    27421 tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata
    27481 acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt
    27541 tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt
    27601 ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa
    27661 tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga
    27721 cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc
    27781 cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt
    27841 ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt
    27901 tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt
    27961 ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta
    28021 ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt
    28081 ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc
    28141 ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc
    28201 ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct
    28261 gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg
    28321 ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat
    28381 ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc
    28441 tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt
    28501 cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag
    28561 agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat
    28621 tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg
    28681 taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga
    28741 atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct
    28801 tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg
    28861 atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt
    28921 gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc
    28981 atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact
    29041 tttttaggat tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca
    29101 ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat
    29161 acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat cgttccatct
    29221 tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc
    29281 ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga
    29341 tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct
    29401 tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact
    29461 gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta
    29521 atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta
    29581 gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga
    29641 acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat
    29701 gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa
    29761 tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg
    29821 aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc
    29881 tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag
    29941 atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc
    30001 tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg
    30061 ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc
    30121 ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga
    30181 tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac
    30241 gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc
    30301 aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga
    30361 aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg
    30421 aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg
    30481 caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg
    30541 atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta
    30601 gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca
    30661 cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg
    30721 ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga
    30781 aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg
    30841 atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta
    30901 ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata
    30961 atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg
    31021 tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac
    31081 attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat
    31141 tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt
    31201 ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca
    31261 ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag
    31321 ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag
    31381 ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga
    31441 tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca
    31501 agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat
    31561 ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca
    31621 cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc
    31681 cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt
    31741 ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc
    31801 gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa
    31861 cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac
    31921 actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag
    31981 tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg
    32041 tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg
    32101 ataaccacgt caagaccata ggattctcct aattttatga catcttcttt tactggagaa
    32161 ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt
    32221 tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac
    32281 actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc
    32341 catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc
    32401 tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt
    32461 ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa
    32521 gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag
    32581 caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt
    32641 tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc
    32701 gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt
    32761 tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa
    32821 aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct
    32881 ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa
    32941 taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta
    33001 acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa
    33061 gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca
    33121 gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg
    33181 aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact
    33241 tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc
    33301 accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt
    33361 gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag
    33421 ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt
    33481 acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa
    33541 catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa
    33601 ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat
    33661 aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca
    33721 aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg
    33781 tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa
    33841 tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat
    33901 ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt
    33961 ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt
    34021 tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg
    34081 tactttattt agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc
    34141 agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag
    34201 ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg
    34261 accaatgatc ggcacaaaag cttccggcca tacttcttgt atgactgcca tcagctcatc
    34321 cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt tacattataa
    34381 atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca
    34441 tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc
    34501 tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt
    34561 tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga
    34621 taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc
    34681 cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat
    34741 gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa
    34801 cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt
    34861 gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag
    34921 accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta
    34981 tattccccac atcttttttc atttattaaa actgcctcc
//
LOCUS       DACBZN010000017        33608 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.017, whole genome shotgun sequence.
ACCESSION   DACBZN010000017 DACBZN010000000
VERSION     DACBZN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.017"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(462..887)
                     /locus_tag="IVD64_000851"
     CDS             complement(462..887)
                     /locus_tag="IVD64_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148122.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1261..2232
                     /locus_tag="IVD64_000852"
     CDS             1261..2232
                     /locus_tag="IVD64_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6148123.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2264..3661
                     /locus_tag="IVD64_000853"
     CDS             2264..3661
                     /locus_tag="IVD64_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6148124.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3820..4443
                     /locus_tag="IVD64_000854"
     CDS             3820..4443
                     /locus_tag="IVD64_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148125.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(4669..5193)
                     /locus_tag="IVD64_000855"
     CDS             complement(4669..5193)
                     /locus_tag="IVD64_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6148126.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5186..5659)
                     /gene="tsaE"
                     /locus_tag="IVD64_000856"
     CDS             complement(5186..5659)
                     /gene="tsaE"
                     /locus_tag="IVD64_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP6148127.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5782..6762)
                     /gene="pta"
                     /locus_tag="IVD64_000857"
     CDS             complement(5782..6762)
                     /gene="pta"
                     /locus_tag="IVD64_000857"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP6148128.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6915..7595)
                     /locus_tag="IVD64_000858"
     CDS             complement(6915..7595)
                     /locus_tag="IVD64_000858"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6148129.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7721..8590
                     /locus_tag="IVD64_000859"
     CDS             7721..8590
                     /locus_tag="IVD64_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6148130.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8616..9131
                     /locus_tag="IVD64_000860"
     CDS             8616..9131
                     /locus_tag="IVD64_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148131.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9178..9741)
                     /locus_tag="IVD64_000861"
     CDS             complement(9178..9741)
                     /locus_tag="IVD64_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6148132.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9796..9975)
                     /locus_tag="IVD64_000862"
     CDS             complement(9796..9975)
                     /locus_tag="IVD64_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148133.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10052..10561)
                     /locus_tag="IVD64_000863"
     CDS             complement(10052..10561)
                     /locus_tag="IVD64_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148134.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10657..11037)
                     /locus_tag="IVD64_000864"
     CDS             complement(10657..11037)
                     /locus_tag="IVD64_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6148135.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11367..12116
                     /locus_tag="IVD64_000865"
     CDS             11367..12116
                     /locus_tag="IVD64_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP6148136.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12300..13577)
                     /locus_tag="IVD64_000866"
     CDS             complement(12300..13577)
                     /locus_tag="IVD64_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148137.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDRIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13656..14300)
                     /gene="pcp"
                     /locus_tag="IVD64_000867"
     CDS             complement(13656..14300)
                     /gene="pcp"
                     /locus_tag="IVD64_000867"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6148138.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14499..15443)
                     /locus_tag="IVD64_000868"
     CDS             complement(14499..15443)
                     /locus_tag="IVD64_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6148139.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15445..16131)
                     /locus_tag="IVD64_000869"
     CDS             complement(15445..16131)
                     /locus_tag="IVD64_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6148140.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16605..16925
                     /locus_tag="IVD64_000870"
     CDS             16605..16925
                     /locus_tag="IVD64_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6148141.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17079..17984
                     /locus_tag="IVD64_000871"
     CDS             17079..17984
                     /locus_tag="IVD64_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6148142.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            17981..18754
                     /locus_tag="IVD64_000872"
     CDS             17981..18754
                     /locus_tag="IVD64_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6148143.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(18935..20359)
                     /locus_tag="IVD64_000873"
     CDS             complement(18935..20359)
                     /locus_tag="IVD64_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6148144.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20570..21016
                     /gene="nrdI"
                     /locus_tag="IVD64_000874"
     CDS             20570..21016
                     /gene="nrdI"
                     /locus_tag="IVD64_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6148145.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21081..23063)
                     /locus_tag="IVD64_000875"
     CDS             complement(21081..23063)
                     /locus_tag="IVD64_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6148146.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23077..23973)
                     /gene="murQ"
                     /locus_tag="IVD64_000876"
     CDS             complement(23077..23973)
                     /gene="murQ"
                     /locus_tag="IVD64_000876"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6148147.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKSGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(23976..25037)
                     /locus_tag="IVD64_000877"
     CDS             complement(23976..25037)
                     /locus_tag="IVD64_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6148148.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25248..25775)
                     /locus_tag="IVD64_000878"
     CDS             complement(25248..25775)
                     /locus_tag="IVD64_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6148149.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(25842..27344)
                     /locus_tag="IVD64_000879"
     CDS             complement(25842..27344)
                     /locus_tag="IVD64_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6148150.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(27602..28723)
                     /locus_tag="IVD64_000880"
     CDS             complement(27602..28723)
                     /locus_tag="IVD64_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6148151.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            28849..29790
                     /locus_tag="IVD64_000881"
     CDS             28849..29790
                     /locus_tag="IVD64_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6148152.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(29940..30836)
                     /locus_tag="IVD64_000882"
     CDS             complement(29940..30836)
                     /locus_tag="IVD64_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6148153.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            31105..32445
                     /locus_tag="IVD64_000883"
     CDS             31105..32445
                     /locus_tag="IVD64_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6148154.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            32663..33106
                     /locus_tag="IVD64_000884"
     CDS             32663..33106
                     /locus_tag="IVD64_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6148155.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT         9959 a         6748 c         6151 g        10750 t
ORIGIN      
        1 aaaaattact acgtgatgta gaataagcct ttctatcgtt tggttatttt atattcaaat
       61 atttgaaata aataaatctt tactctttga tttgtaaact gaaatgatcc actgtttgtt
      121 ttactgatga agcttgatca aaggaagaag ctgagtttga agttcataaa aatcagccat
      181 agtgagtttt gcttggattt gtttatcttc ttctactttt tacataattt tgaacaggat
      241 aagattcaaa tgttttaaat gaatctttga ctagtttgtg acagttgtcg catatgctca
      301 agcttccttg gtcattaaat tctgtatgga tatttttgtt gttattacat gtacaataat
      361 caggactatc gatcatccat ttcgtctcct ttacaatcat cttgcttctt tactatagca
      421 gaaaagaagg ggagaaacaa aagaagatcc aacagacggc actatagact gtagatgttg
      481 ccatcctcat ccttcaaacg gtaattaccg atttttgtaa atatgactgt tgtttctttt
      541 ttattttctt ttcctgaaat aatatattcg tcatctttgg tagtgatctt gatatctgtc
      601 agcactgttt tttcttcaga tgattcagaa gaatcagttc cttgtgtctg atacgtgcgt
      661 gtccattcca tttttttatc tgtaaatgtc aactgtcccg cggataaatc catatcttta
      721 cgtactttta gcgtatatac actgttggtg gtatctgctt tttccgcttt taagacagaa
      781 gaatcggttg tttcgtccga ggattggtca gagccgccaa acaaatcaca gccagtcaat
      841 gtcaaaagtc ccgcagacaa aataaatagt acgattaatt tcttcaaaat acttccccca
      901 tttgatagga taaagaaatt ataccattgg aatgtaaaac ttcaaacgga tggaagagaa
      961 aaacgcacaa aacacccaca aatagagtaa attttagaat aaaattttaa aaaggcaaaa
     1021 aatccgccac tttcagaaag cgttttaaaa aagtgttgca atcttttaaa aaaatgctat
     1081 attaaaaatg aaccggttca aatacttgtt taagtataat taatagtgag aggaagttgc
     1141 tctactgaag actttagtta tatggatatt gatgacaagc aacactatgc aaagaatgcg
     1201 gtcatcataa tcgaacaaca agaaagagaa atcaaatgta actggcagga gtgagaatgt
     1261 atggcatcga tccgtgatgt ggctaagatg gctggtgttt cagtgggaac agtttcccgt
     1321 tacttgaatg gtcagcaact gaaggaaaag aacatgaaaa aaatagcaga agcgatttca
     1381 gcgttggact ataaagaaaa catcattgct aaagggttga agaataatcg aagcttctct
     1441 attggacttt tgataaacgg gatttccagc cgttttgggt cggaagttgt ctcagggatc
     1501 gaaagagtag ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca
     1561 gaaaaaattg aacaaaaaat tgaatacctg atgaagcatg tgatcgatgg gctgattgtc
     1621 ttcttatccg aagaagagtg gtctggattt gaaatgctca caaaaatgag tattccagtt
     1681 atcgcattga attgtccaga aggaccagca ggtattgata caatattagt gaatgatcga
     1741 gaaagtgtag caaaagtgat cgctcatcat ggtgaacaag gacataaaaa aatcggattt
     1801 attgctgcca ctcaaacaga ctacgtagca agagaacgtt tagcaggagc caaagaagcg
     1861 gtccaaaatg atccatcgat ccaattagag gtctttacag gagactattc tagaaaaagc
     1921 ggctattacg gggcaaaagc attattagaa aaaggggcaa ctgctatttt tgtcagcaat
     1981 tacaatatgt cgattggtgc catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag
     2041 gatattgcgt tcagtcacta tgattattta gataaaaacg atctgagtat ccttccgaaa
     2101 atcacgatca cgccaccaac agataaaatc ggggaacgag cagcagaaca gctgttaaaa
     2161 cgaatcaaaa aagaagagga agaaaatgaa aaagtgattg tgatggaaaa tatcatacat
     2221 ggaatcaact aaaacgaact aaaaaagaaa aagaggtgct ttgatgaaaa aatatcaatt
     2281 tccagcaaat ttttggtggg gttctgcagc aagtggccca caaacagaag gaaggatttc
     2341 aggcgatgga aaaggagaga acatttttga ttattggtac aaaaaagaac cgcaaaagtt
     2401 ctttgatcaa gtaggacctg aaaaaacctc gcaagtttat acgaagtatc aagaggatgt
     2461 acagttgatg aaacagactg ggcataattc atttcgaaca tctatccaat ggagccgttt
     2521 gatacctgat gaatctggaa caatcaacga agaagccgtt cgtttctatc atcgttattt
     2581 tgatgagttg attgaaaacg gtatcgaacc ctttgtgaat ctgtatcatt tcgatatgcc
     2641 gatgtatctg caagaaaaag gcggatggtt gaaccgagaa acggttgaag cctatgaaaa
     2701 atatgccaaa agttgttttg aattgtttgg ttcgaaagta aaaaaatggt ttactcataa
     2761 tgaaccaatc gttccagtcg aagcaggtta tttatatcga tggcattatc cagaagagtc
     2821 agatatgaaa aaagcgatcc aagttggcta tcatgaagcg ttggcttcag cgttagccat
     2881 caaatcttac catgaaatgg agcaaggtgg tgttattggt atcatcttga atctgacacc
     2941 aagctatcca agagacgaat caaaccctga agatgttaaa gcagcgcaaa tagcagatgc
     3001 gttcttcaat cgttcattcc ttgatccagc tgtaaaaggc gagtttccaa gtgaattgat
     3061 tgagatagta aaagaacttg atctcatgcc tgtgatccat gcagaggatt tgacggttat
     3121 caaagaaaat acagtagatc ttttaggtat caattattac cagccacgcc gggtaaaagc
     3181 aaaagaaaca ccaatcgata ctaaaaacgg tccaatgcct gaggattatt tcgacaatta
     3241 tgaaatgcct ggtcgtaaaa tgaatcctta tcgcggatgg gagatctatg aacaaggaat
     3301 ctatgatatt ttgatcaatg tacgagatca ttatggaaat atcgactgct ttatttctga
     3361 aaacggtatg ggtgtagaaa acgaagcacg atttatcaaa gaagatggca tgatccacga
     3421 tgattatcgg atcgaatttg tccaatctca tctgaaatat gtccatcaag ccattcaaga
     3481 aggtgccaat tgtttaggct atcatatgtg gacatgtatg gataactggt cgtggaccaa
     3541 tgcatataag aatcgatatg gctttatttc agtagactta gcacaagatg gaaaaagaac
     3601 aatcaaaaaa tcgggtcatt ggttcaaaga cgtatcagat aaaaacggat ttgaggctta
     3661 agaaagaata gttgagagca aatgcgtgaa attctccatt cttaccattt tgtgaaaaag
     3721 aagttcaatt atttacgatc tttttcaact tttttgtctt tctatttcat tttttgtata
     3781 taatagaata gtcaaaatta aataggagtg gaggaaagca tgaggaaaaa aagaccgttt
     3841 cttctctttt ggttattcgg ttttttctta ctgcttattt tatccgcagc attttcagga
     3901 aaaatgacag cagatcttgc tcagaaagaa acagaaaaaa tcgtagcaag caaagaatca
     3961 gtagataatt tcaaaaaaga attctcaact tattggtggg cggagtataa aataactgcc
     4021 aattacgaaa aaggcgaatt ggacatttat ttgccaaaag gaaaagaaga aaatcaattt
     4081 attaatatcg atgacgtact tagtgcgatt tctctcagac aatatcaatt taaagacaag
     4141 catttgaccg ttttgctttt gactcatgat ggcgacaaag tagcccgttt gaaaaatgga
     4201 aaagtgatca ccgcttattc tgaaaaagta gacaaaaaag tggtcgccga tcgtttagcg
     4261 gaatggaaaa agacaaatga acaagtcaag aaaacaactg tctcttctca aaaaaacgag
     4321 tcgacaggaa caagtccttc agattctggc agtggacaaa cgataacggg aagtgaagaa
     4381 aatcaagcct caattattac gaatgagtca tcacaaggac aatatcaatc ctatggacaa
     4441 tgaaaaacag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggcaa
     4501 aatagttcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
     4561 cgtttattca aaaaaaatgg actgcgacac gacttttgtc acagtccctt cgtgtgtttt
     4621 tctgccttct caaaaggatg acagtttctc actcttttcc tatttcttct attcaatcaa
     4681 ataactcatt ttatatacat ctaaaaattc acctagatct gttcttgccc ctctaggcat
     4741 gaccgcttcg atttggaagc ccatttttcg gtaaaggtgt acggctcttt cattacgaac
     4801 ttgaacgtca agctccaatc gaaaaagtac acccatttcc ttcgcccaac tgatgatttc
     4861 ttctagcata agagtgccta gtcccattcc ccaatattcc tgcagaatac tgataccgac
     4921 ttctcccacg tgagacaaac gaaattgact gtctgccttg acagatgctg ttccaataat
     4981 cgtcccttca tagattgcaa gcagcaataa attattattg ctttcgtata tataatccaa
     5041 ctcttcttct aaggtttctg gtggtaacag gagttctgct tcatccaaaa ctaaaaattc
     5101 tgtctcctta ttaacttgtc ccatcatcaa aagaatgtct tttgcatctc ctggcactgc
     5161 ttcccgcaag atgatcccaa cttcttcact catgattttc agcccatttt tctagaattc
     5221 gctgtttgaa ggtttcactt tgctccccgt atgagattag tttcacatac cgcttttcta
     5281 aatctgtatc acttttttct aaaatcagtt ccaaataatc ctccggcagt gcttcttcta
     5341 atagatttcc ccattcaaca acggaaagcc catcaccttc aaaatattca tctaacccta
     5401 aatctgctcc gctagctgct acccgataaa tatccatatg atacaacgga atccgacctt
     5461 gactatattc tctaataatc gtgtatgtcg gacttttgat catttgcgtt atccctaatc
     5521 cttgggcaat tcctttcgtc aacgttgttt tacccgcacc tagatcacca gtcaatatca
     5581 gattgtcacc tggcattgcg gaatgaccaa ttgcctctgc gaattgttct gtcattgtta
     5641 atcctgatag ttcgatcatt tttcatcttc tcccgctctt ctttcttttt gccagtatat
     5701 cgcgtaatga agttttttta aagaaaaaat gaactagtca agaacagcag ctgctgtttt
     5761 tggctagttc gcgtgtattt gttaatcaat caatgtttgt gctgctgtga taatcgataa
     5821 tttatataca tcttcttcat ttgctccacg agacaaatca gaaaccggtt tgttcaagcc
     5881 ttgtagaatt ggtccgattg cttcaaagtt accgaaacgc tgagcaattt tatagccgat
     5941 atttcctgac tgcaattctg gaaatacaaa gactgttgct tgaccagcta cttctgaatc
     6001 tggcgctttc agttgtgcaa ctgaaggaac ataagaagca tcaaattgca attcgccgtc
     6061 gatcatgtat tgtggcgcta gttttttagc gattttagtt gcttcaacta ctttgtctac
     6121 ttctggcgat ttcgctgagc cttttgtcga gaagctcatc attgctactt ttggttcgat
     6181 atcgaacaat tctgctgttt ttgcagaatc tacggcgatt tcagccaatt cttgcgcagt
     6241 tggattcacg ttgatcgcac aatccgaaaa gacatatttt tcttgatctc ttccacgtac
     6301 catgatcatc gctccgcttg tccgactgat gccaggcttt gttttgatga tctgaagtgc
     6361 tgggcgaaca gtgtcgccag tcgaatggat cgctccagag accattccgt ctgcaatccc
     6421 catatacgta agcatcgtac caaagtaatt cacatctttt aaaatctttt ctgcttgttc
     6481 tttggatgcc tttccatttc tacgttcaac aaaagtatcc accatttcat cccacttatc
     6541 gtaattatca gggctgatga tcgtaaagct agatgttttg attcctctgg catgcgctgc
     6601 atttcttatt tcttcttcgt tccctactaa aatgggttcc acaagttctt ctgctttcaa
     6661 ccgggcagct gcgcctaaaa tacgagcatc agtagcttcc gggaaaacga ttttgatccc
     6721 acggcgaaca attttaaatc ttaaactatc aaacaattcc acaagatgac ctccagacgt
     6781 ttttatatgt attatcatac actattccag aaaaaaagaa cagtttattt tatctgtatc
     6841 agtcaaaaat gctggaaaaa ttaagttttt cacatacaaa gcgaactgca ctataacagg
     6901 aaagcgctgt tttattacac tgtttcaggc agttgccaat caatcggttc ttctcctaac
     6961 gcaaggagcg catcattcgt tttcgagaag ggtttggaac caaaaaagcc acgacttgca
     7021 gataacggac tgggatgtgg agatgttatg atgatatggc gactcttatc gatcattttg
     7081 attttttctt gtgccggttt tccccacaag ataaacacga ttggtttttc ccgttcattt
     7141 aatttttcaa tgatcgtgtc tgttaatctt tcccaacctt tcccgcgatg agaatacgct
     7201 tgcccttccc gaacggtaag aacggtgttg agtaaaagga ctccctgttt agcccatgac
     7261 actagatttc catgttgaac aggtgcgatt ccaagatcat cataaagctc tttatagata
     7321 tttcttaacg atggcgggat ctttacgcca ggttgcacag aaaaagataa gccatgagct
     7381 tgattttctc catgataagg atcttgtcct aaaatcacca ctttaacttc ttcataagga
     7441 gtcaattcca atgcttcata gatatgatac atatctggat ggatcttttg ggacgcatat
     7501 tcttttttta aaaattctct tagatgcaga taataatctt ttgaaaattc tgaggacaaa
     7561 acttcttgcc aactgttatg aatgatttct ttcaaaaact ccacctccta ataaaaagaa
     7621 taactcacat tttcttcaat cacaagggac aatcttcacc ctcagcactt gattaatatg
     7681 gtaaactagg aacaagaagc agaaaggaac gataatcatt atgatcaaat taattgcatc
     7741 agatatggac ggcacattat tagatgccca tatgagtgtt tctcctgaaa atgccgaggc
     7801 gatacgtttt gcaaatgatg ccggtgttga gttcatggtc gctactggtc gaaactatca
     7861 agaagctcgc gcagctttag atgaggtagg aatcgattgt gcgatgatca ctttgaacgg
     7921 ggcacaggta tttgataaag aaggaaattc attgtttacc gtgtcgattc caaataatca
     7981 agcaatctct gttttagata ttctagatgc taacggtatt tactatgaag tagcaaccaa
     8041 cgatggtctt tattctgaaa atcaagcgaa acgaattgaa agttttgctt ccatggtggc
     8101 tacccattta cctcacctta cgtataaaat ggcgattgcc atggcctcag ctaagctgga
     8161 gttattgcac atcacatacg tggacagtat tcgtgctatt ttggaagaaa agaatctgga
     8221 agttttaaaa attatttgtt ttcatacaga agggccagct gtattaggtc cagttggaaa
     8281 gcagatttct cttttaggcg atctagccgt tacttcctct ggtcaaaaca atatcgaagt
     8341 gaaccataaa aacgctcaaa aaggaattgc tgttgcccat gtcgctcacg agcgtgggat
     8401 ttctttagaa gaagtcatga cgattggcga taacttcaat gatgtcagca tgctgcaagt
     8461 agcaggagtc agtttcgcaa tgggaaatgc agaaatcgaa gtaaaagatt atgccaaata
     8521 tacaacggac acaaaccttg aatctggcgt aggaaaagcc atattgcgcg cgattgcaga
     8581 agacttgtag ttttatctag aaaagaggga tatagatgtc agaatttttc attcaggaaa
     8641 agcagcttgg acgtgcaact cggacaattg tcaaaaacga aaaaggacat tccctgtttt
     8701 tgatggttgg tcgctgggga acaaagggcg atgcattgtc tctttatgcc atgaatggcg
     8761 atctcgttgc tcacatcaag cagataagcc tgacattcgg tacgcgtttt gagctgtata
     8821 aagagttcga aaaggtggga accatgcaga aaatattcaa ttggcccggt gacttttatt
     8881 atattcgtag acttcactgg acagtccaag gtgatatcta taatcatcaa tatcaaatcc
     8941 atcatttcaa tcagcaaatc atgaaaatgg ataaagcaac acttttttca ggagattact
     9001 atgtattgga tgtctcaaat gatgaagatg ctccagtttg tatttgtatt gctgcggttt
     9061 tagattactg gctctaccgt aaagacaaaa gtcgcgataa gcctgtttct taccgactta
     9121 acttaagtta aaaagatgta cctgaaatgc ggaaatacgc atttcaggta catcttttta
     9181 gatattcttt tgcatatgat cataaagatg accactgatc atccgctgac cgacaacttt
     9241 gaagtcatgg cgttcgtata attttttagc ctttggattt ttttcatcca cacttagacc
     9301 gatgacggag cgattagctt tcttcgccaa tttaggtaat gcttctaata atcgcgaacc
     9361 gatgccttgt cctctaaaat cttctgatac actgatagag tccaaatacc attcgttggg
     9421 aaacgtttct ttatcaatga acaaacgtgt agacgcatct agctgatatt tctctaagat
     9481 aggtaccagt ggctgatcga tgattgattc ttcttcatct gtataaccgt aagcaactcc
     9541 tgcaacttta ccgtctattt cttctacgat acctcgtgaa taactgtaac gataatttgg
     9601 atcagtgatt gcttcttcta aaatctctaa agtcttttgt tcccctgtct gcttcaaaaa
     9661 tggcagttcc atatctttta gaatcactaa gatcaatggg gcgatttctg gagcatcttc
     9721 ttttctcgca aaacgaatca tagtttcctc tccttttctc cattctaaca tgcagaaaaa
     9781 gaaatcgcaa aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga aataaccatg
     9841 agtaacgatt cttgccgctt cagttcggca ataagggcag acgactggca tttctttatc
     9901 attatgttcg actacattat tgacttcttc gtatgcaaat ttttttccgc aattagggca
     9961 tttcattttt tccatgttat ctcaacttcc tttcatcttt actctcttag cttactctta
    10021 tattgtaaaa aagagaagca atatccttct tctatgcaga tatccatacc tgacggtcct
    10081 gttctccatc tattaatcgg actttataat aaggacgttc atctttgact agaagtgtcc
    10141 actctttcac ttttgctttt ttcccgtagt agctgactgc ttccttcatc gctgcttgcg
    10201 gttgtttgaa ctcttccgtt ttttctatat caaaaagatg ttctgcggcc ttttccgtgt
    10261 catttcgtac ggttgttttc ccagtggatg gattgactgc tatgacttgt ccttcacaaa
    10321 aaaactggta ttcatatgtt gcattcgtag gattctccaa atctttttca actgcaaaat
    10381 gaatagcaga tatgttatgt tcttttgttt cgttttttac gattgctgct gcttttttca
    10441 atgagatccg atattcctgg tcactaatat catccatgtg aaaagcggca ttgagcagtc
    10501 ccgtcatacc ggtaatccct attacgatga gggcagcaac accaaacatc atttttttca
    10561 ttcttcattc tcctgtttct tttttttaaa gaatatcaag aaatgaacga atgaaaaatc
    10621 gaatgaaacg aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt ctaatggcaa
    10681 tccgtctgga tcaaagaaaa acgtcatttt ttttcctgta aaggtatctt ctctaatcgg
    10741 ttctgattcg atccctaagg actgcaattc tgcaattgtt tcttcaatat cgtctacttt
    10801 gaaagcaaga tgacgcaagc cacaagcttc tggatagttt ggtcgttgtg gcgcattagc
    10861 aattccaaaa atctccaatt cagcacttcc tagtttcaga tctaatttat agtcatctcg
    10921 ttctttgcgg taattttctc tgattacttc aaatcctaat ttattaacat aaaaatcacg
    10981 tgatttctga taatctgaaa caatgatggc tatatgatgg atcgttgtaa ggttcatatg
    11041 agcactccta ttcttcgttt aagctcggat ctatattaat ctccagttcc attgtaaatg
    11101 tgtcagattg aaaaaaatag ctgtttttcc tttttataag aaaaaaggca aaaaaaacag
    11161 aaagtaggtc ctccaagaca ctcttcctgt tcccaatata ataaatgaaa gtaagaaatt
    11221 atattttcaa gaaaacaaac agaaaattat cataacacaa attatttata attgaaagac
    11281 tagttttttt tcaagaaaag catacaatag attgtgttag taaatacaga acacactaca
    11341 tttagtgtct taaggagtcc tcatacatga agtcatattt tcagttttta gctcatcagc
    11401 taaaaggctg gccgcaacaa aattattatc ttttcttttt cagtcttggc tgccaggtca
    11461 tgactcttgt caatagtccc atcacttggc tgtccgttat tacatttatc ggtaccacgc
    11521 ttggtgtatt gtgtatttta tccatcaatg cggcaaaatc agtcaacggt attttaggta
    11581 ttttgtctgc gatttgtttt attatcgttg ggttttcagc aaagaactat ttaagtatcg
    11641 gtgaacagat tgcttatatg atcacattgg atatccccgt attgttaagt aaagaatgga
    11701 atcataatat ggcttcaaaa atccgaaaat tcactggaaa aagctggttt atcgcaatca
    11761 tcagtacttt ccttgtctat cttgtttcag gattcctgat tggtcgttat acagatgatc
    11821 ctcggccgtg ggtagatgcc attgcttttt ctattagctt gactgctggt atcatcagtt
    11881 tcttgcgtta caacaaccaa tattactggt ggttagcttc agggcttgct caaatggtcc
    11941 tttggtatat ttcatttacg catggatctg caagtttggc gatgttcgtg aacagctctg
    12001 tttacttgct gaatgatgtc cttgcattta ccgtttcacc ttggtataac caaaaagaac
    12061 gtgaacgtat gcaaaaaatg gaagaagaat actacaacgc acaacaacac gcataaaaaa
    12121 actgagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    12181 gcggaatcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    12241 ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctattttt
    12301 tatagtaatt ccttcagata ctttcttgcc tcatcattga aacgcagtga ttcttctaac
    12361 ttctgataat attccaacgc ttgtttctga gcttcagtaa aacgataatg ttttcctttc
    12421 catttgtcga ttgtctcttg cacatttcca gattggatat ctttcactac tagttgccat
    12481 ttttcttttg agactgtacg gtctttctta tcagcagatt gtatagcttc tcgttcttta
    12541 cgcaatactt ttaaaaaacc atttctgact atccgaggaa tgcggtcgaa gaacccttct
    12601 gtaaaaccat agtgacaata ttgcttccaa ataaatagat agatttttgg ttggatgaat
    12661 cgttggtttt tcgcaaaata ttccatctga gcagtgactt ctggtgttcg ttgacgtttt
    12721 gtctttctca acacggaaat aaatgtttca aaataagcat taggatgtac tggcacttct
    12781 tcatatacga tggattgcca atctttacgt aaaaatgcgc gatagttgat caagaattct
    12841 tctacatcct ttactaattc ttctgcaaag tcgtcaaata gttctttttt tatctgtcgc
    12901 ttttttgact gcccattgat gtgttcactt attttcttga tttcattttc caatgcttga
    12961 taatccgcta tgtaaatcgg catttctact tggcgaaatt ctgcgagccg ctgagctaat
    13021 tttatatccg gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac aaaacagtat
    13081 ttttgccata gtccttcagg aaaatcgata ttcttctgct taagccataa taattcgtct
    13141 aaggccagat caacttttgt cattccctga tggatcgaag ctgccactgt cggagagaca
    13201 tgcaggtgat cggcaaagtg gttgatgcct agttttagga tctttccggt ttttggcgat
    13261 ttaacgatgt attgcacttt taattctcta ccacactcac aatacaattt ttgctggctt
    13321 cgttttgttc gaaaaaacgg attgatcttg aagtcagaaa acacccaatc tgttgcagct
    13381 aaataatgtt cttccataaa acgagagctg attagatatc ggcggtgctt atcgactagt
    13441 tcccgctgtt ttttcgttaa ttttgcatag atctcccaac gttgactgct tgtgtattgt
    13501 ttagaaattt tgatgggata gcttttgctg atttcttttg gaaaccattc ttcaaagacg
    13561 gattgccatt cttccacggc tgtttctttc ctttctaatt acaaaaagct ggctactaac
    13621 ataacgttag caaccagcca gtgtttcttg ctaaattagt gagttgttcc ggcatttccg
    13681 gaaatatctt ctttattttc tactgctgct tcaactgcat aagtcaaaga agaagcaatg
    13741 tcttctaaag acatagaagg cgtacccact ggacgatcga tcacttgatc gggtgagaat
    13801 ggcacgtgga tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa atgaagcaaa
    13861 cgatacatga tatcattgca gacaaatgtt cctgctgtat aagagatgta cgctggtaga
    13921 ccatgatctt gtacattttt tgtcattgct ttgattggca atgaggtaaa gtaagcggct
    13981 ggaccgtttt cttctaaagc cgtatcgaat ggctgatttc cttcattatc agggattctt
    14041 gcttctgcta gattgattgc gacacgttcg aacgagactg ctgaccgccc acctgcttgt
    14101 ccgacacaga tcacgacatc tggttggtgc tgttggatgg cttctttgac tacttgactg
    14161 cttttttcga atacggtcgg tacttccact ttgatgattt cagcaccgct gatttcatct
    14221 ggcatttttt ttactgcttc ataggctgga ttcactttgt ccccaccaaa tggatcaaaa
    14281 cctgttacta gtactttcat ctgttgttcc tccgtccgat tgatttaaca tatggttatc
    14341 ttttatcata ccattttcct tgaacagcgt aaataaagga aacaatagtt taaataacat
    14401 aacagatcaa acttaatata ttcttataaa aaaatagtgt tccactacaa tgttagaaga
    14461 ggttgcgaca attattctgt cacaacccct ctcttctatt atttaaatag aatcatataa
    14521 gcaatttgga aaactagcat aaataacgca ataaacaact ggtttttgat cactccgtat
    14581 ttgtctttca tctccagcat cgctacaggc acgatattga agttagcagc catcggtgtc
    14641 agcagggtcc cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc tggattcgct
    14701 ccatgactaa atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat ggccgcaaat
    14761 gcattcccca tgattaccgt aaatatcacc atgccaagag cgtaaatgat gatgccgatg
    14821 ttcacatttc cttctggaac gatcgtacct actgcattgg aaatcactgt cccaactcca
    14881 gcgcttgtaa ataccgcacc tagagaagct aaaagcattg gcaacatgct taacggccct
    14941 actgtgccca gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt atctcgagaa
    15001 aagaacatca agattagcat ggctgcaaaa acaccgacgc ccacgccgac taaagcacct
    15061 aaattggtaa atagagcaaa caagatcgca aagacaccga tactcaaagc tggaataaaa
    15121 attttcattc ctagtttgtc tgacattttt tccatatatt cttttgatgg aagccggctt
    15181 tctcctttgc tgactttctt agcaattgct ggtatggtca tcgcaaagat caagccaccg
    15241 ttcacgattg ccggaagcca tcttcctcct gcaataacta cccctaagac cgtccagaaa
    15301 aaggctgacc cataacgatg aggattggtt ttatctgtta aatttcttac tccagtgtaa
    15361 atcaatacga gtcccatcac gatataaagg atctccagta atttatcgcc taataaaata
    15421 tcaggatttg taaaaaagtc catcttattg cccctttcct ggataatatt tcttcgctaa
    15481 tttcttttct ttaagataga agtagacact tgccacaact aaagccgtga tagccactgg
    15541 aacttccact aaagcaagct gtcccaatgt tacgtcatat cctagatctt tcagtgttga
    15601 ttggactagc aacgcccctg ctcctccgat gaatagtact tggccaaaga accagcagac
    15661 attttccata gctgaagcca taccttttag ttcttcttca tgcttaggat ggacttctaa
    15721 cccttttgat tcgatggtcc ccaatgccat cggtaaaatg attggacgaa caaatccagc
    15781 aactccacca aaactcacgt taaaagccgc aaagatcatt cgaaagatcc cataaatatc
    15841 tacaatcata ccagcagata ctttcttgaa tcgcttgatc aaggaagccg ctgattcttt
    15901 cagaccattt ctttcaagcg ttcctgttgc caacataatg atgataaaaa tcgccatccc
    15961 gcgattgttt acaaaacttg tacctaaaag ttctaacatt ccttgaatgc caagaccgcc
    16021 agtcaaagct gtaacaacta aagcaaccat tacgatcaat atcgagtcta atttaaatgc
    16081 aaacccgaga ataacaatca agacgcctaa aaggctaaga taattttcca tatattttcc
    16141 tccataaaat gacaatattt ttttaattat aaagcaaaac acataaaaaa tatagttgtt
    16201 ttctcacgaa atagacacta tttttaaata tttttcgtta tttttttatc acaaaaatta
    16261 tcttacttat tgtaaggtaa agcttttttt aatttattgc ccgataatat atagaaataa
    16321 ggtctagtta ctgttttttt taaatgatta ataaataaca acttctttct atgaaattgc
    16381 aaagcattca tcttcttttc cgctaacgat atatccgtca gtgcttggta cactttacta
    16441 aaaagtatga gattgccgat atctggatag gtgtgctata ctacagaaaa agatatacaa
    16501 tctgccaccg ggtaggataa agaggatgat cattccatta ggtctttgaa gggatgattt
    16561 tcctcttttt gtgtattttt ttatagttaa ggaggaatgt aagtatggca tggatggaat
    16621 taatcatagc aggaattttg gaagtattct ggtcaacgat gatgaaatgg agtgatggtt
    16681 tttctaaaat caattattct ttttacacag tgattggcat gattgctagt ttttactttt
    16741 tgtcaaaggc aatcaaaagt ttgccgatga gtctctctta tcctatctgg acagggattg
    16801 gcgcagttgg ttcggtcata attggtgtgg tcttgtttca tgataagctg aatctttcca
    16861 cttggttctt tgtggggatg ctactcatca gtatcattgg catcaagatt actagcggcc
    16921 actagacact tggaataagt actgatcaga aaaaataagc ctttttattt aatattcaat
    16981 ttttttatga aaaaataaga aataagtcca atgacctagc gacttctcca ctcatcaggt
    17041 gtatgcttgt actatctata gaaaaggagt gaatattttt gaaaagtctt tcggtccaag
    17101 aattatccaa gaactttggt cacaaacaag ccctcagcga tgtcagtttt agcttatccc
    17161 ctggcaaaat cgttggatta gtaggtccta atggcgctgg aaaaacaacg atcatgaaag
    17221 ctattttagg actgatccat tactcagatg gaacaatctg tattggtgaa aatgagattt
    17281 ctccctctag tcataaaggg ttggaacaag taggagcatt gatcgaatac ccgggtatct
    17341 atccattctt aacaggctat gatcatctga agttattctc tgaaaccaac gatgtatctg
    17401 ctatcgacac tatcgtgaat cagctcaaga tggaaaagta cattcacaaa aaagcgaaat
    17461 cctactcgtt gggaatgaaa caaaaattgg gaattgctct tgccttactg aaccatccgt
    17521 ctatcgttat tttagatgag ccgatgaacg gtttggatcc acaagcaaca agagatgtgc
    17581 gaaaaatcat taccgacttg gctactcagg gcacctcttt cttgatctcc agtcacttac
    17641 tcagcgagct tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga aagatcatca
    17701 aacaatgtac aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt atctctacaa
    17761 caaatgatct agctgctcga acgatcttga cagaagctgg atatactttg gaagatcctg
    17821 ataaaatcaa gcttttaaaa gaaaacgaag aattattagg aaccgtgatc cagctattgt
    17881 ctgcacaaaa tatccatgtg acagacgtcc agcatctgac gaatgatttg gaatcttctc
    17941 tattggaaat tctggattca gtaaaggggg aagaagaaaa atgacattgc ttgtaaaaca
    18001 agaattattt aaattgatca agaaaaagtc aactgctgtt ttatctgttc tattagttgt
    18061 tttgctcata ggaacagcac ttctagccaa aaaatacacg acgatcattg atccagttga
    18121 aatgactgct cagcttttta gtgccacatc ttggattgtc tttatcatga ttgcagctgc
    18181 aagcacgatc atttcgatgg aagcacaata tggtacattg aaaaatctcc tttatcgaaa
    18241 gtattctcga ggagaaatat tagtaagcaa atggatcaca ttagttattt attctgttta
    18301 tttatacctt ttagctatta ttgtcactgt gttgatgaaa ctgattcttt tcccttcgat
    18361 ttccttcacg gagaaggttt ctactggtca aacactgatc caaagcttgt tcacttatac
    18421 tttgggtagt tatatcggtt tatggctgat tttaagttta gtgttgatga ttgcttgctt
    18481 tatcaatagt tccggcgcat ccatctcagc aggaatcgtc ttttattttg cttcgtcaat
    18541 catatctggt atccttattg cactcatcca aaaatgggag tggatcaaat ggaatccgat
    18601 cagtatgttg aatctgcaaa accaaattgg aaacgaagag atcatgaagc aattgactca
    18661 tctttctact aatcaaatgc tattcggtaa tttagcttac atcgttttgt tcttagcatt
    18721 aggatatctc gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag aggctgtgaa
    18781 agagacgttc agccttattg ccgattggct gtctcttaaa caccgtttat tctgaaaaag
    18841 gagctgtgac aacatttgtg tcacagctcc tttcctagta tgtttttgaa taaacgacag
    18901 gctcacaacc tctctttata cttcttttat acttctatac gtgaagcatc ccagtggcaa
    18961 ataaaaccat tgcaatcact ccgaccatcg tcacacctat aagtaaaatc ttatctaagt
    19021 ggatatgttg tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa taaatgcctg
    19081 gcaagaaaag aacaagaaca agcattactt cattaaaggc tacgactagc atagcccata
    19141 cttgaaaggc acttgctaaa agccctacta aaaattggac ccattctttc cgctgataag
    19201 aaagtttcat ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct gctgtaccaa
    19261 gtgtgtaagc aaagttataa gcactgtcag tgaataataa tgaaagtaaa aagacattcg
    19321 tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc
    19381 atgctttagg caaaagtccg tcttcagcca tgtttcccat tgtttcaatc ggtaagatcg
    19441 tccatgagag ccaaacacca actaaagaaa tgatcaaacc gccgttgatc acgactgctc
    19501 cccatttacc aaccatcatt tcaaacacat agcccatagc aggctgcgcc ttgatcgttt
    19561 gcagctgatt catcgtcatt gtcccatatg gaagcatgga aaccaagacg taaataataa
    19621 tcaagccgaa tatccctaga atcgttgctt tcccaacatc tgattgtttt tttgctcgtg
    19681 aacctacgac agaggctcct tcaataccaa caaataccca aagcatcacc atgatgcaac
    19741 tcaatacttg actggaaaac gggatcggct gtccattcgc attcaatata atgtcgctag
    19801 aaaagttatc aatgaattgt tggatcgaaa acgtgctgat agctactaca ataaataaaa
    19861 ataatggcac taatttacag aatgtgacga tagcattgac gaaactagca ttttcgactc
    19921 cccgattcac tagccatgca tatccccata aaaagactgt actcatcacg atcgatgcca
    19981 gattttgccc accttcaaat actgggaaga aatatcctaa tgcgctcatc aacaatgttg
    20041 caaaagctac gttgcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag
    20101 cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt
    20161 ttttattgat attattgatc gacaaggaga gcatcaacat tgctgttcct acgagtaccc
    20221 aactgaaaat agctgggccg ccagccgatc cggagatgtc cgtcataata ccaaatacgc
    20281 cacctccaat cgctgaacca attactaagg cagcaagtgg aattaaacca attccttttt
    20341 tctgttgctc tgtatccatg taagcatcct ccaaaataaa accgctttta tcaaaaagta
    20401 taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac
    20461 cggctttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa
    20521 aaacttaaaa aagatgctat actaaaagga aagaattgga ggatgatcca tgaacatttt
    20581 atatatatcg atttcaggaa atacgcgtgc ctttgccaaa catctagcag aatatgctga
    20641 aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa
    20701 tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg
    20761 cggaaatggt ttagataacg gtgatacaga aatcttgacc gaaacgatgc gtgaatatct
    20821 ggaacatgaa gacaatcata aactttgtct tggtgtagtc ggtagcggaa ataaaaactt
    20881 caataatcag tattgtctaa cagccaaaca atatgcacaa acatttggct ttcctttctt
    20941 ggcagattat gaattacggg gaacaccatc tgatgtgaca cgaatttatc agattttagc
    21001 agaaaacaat aaataataaa aaagaagaag ctgaaaaata gatttcagtt tcttctttcg
    21061 tttctattgt ttctatcata ttaagctttg acatccatca caggtgcttt tgcttttgct
    21121 ttacctgttt tttccaagac aaagtttttt acacgatcca tattggttac caagacgatc
    21181 atggatgttt ctttgcctgc tgctttgatc gcttctaagt cagcttctgc tacaagcgta
    21241 tcaggtgtca cttgctgccc ttctgttact ttcacatcaa atggcgtacc gcctaagtca
    21301 actgtgttga ttcccatatg aagcaagatt tctaaaccat ttggcgttgt gatcccaata
    21361 gcgtgtttcg ttgggaagac agagctgact accccagtaa ctggagaata gatatgtccg
    21421 ttttctggtt ctacaaagta gccttcaccc atcatctttt gtgcaaaaac tggatcactt
    21481 gctttatcta aagaaacaac tgttccgtca gcaactgatg agaggatagt cacactttca
    21541 gctgaagttt gaggtactac tttttcaggc acttcattta ctgtggctgt ttcagtcgct
    21601 aattcttctt tttctaaacg gtctttagga ataccgaaga agaatgtagc aacgaagcca
    21661 ccagcataag ctgccaataa tccaaggatg tagcctaacc atttaccgtc agagatcaat
    21721 gggatcaaag cggctcctga aggtccaata gcgatagccc cgacatttcc gatcattccg
    21781 atcactgccc cgccgatacc tccaccgata caagcagtaa taaatgggcg tcctagaggt
    21841 aatgttactc cgtaaatcaa tggttcgccg attcctagga tcccgactgg taaagctcct
    21901 ttgatcattt ctgttaattc tttatctttt ttgcagcgaa tccataatgc caatgctgca
    21961 cccacttgtc ctgcaccagc catcgcaaga attggaagca atagcgtacg accagtttca
    22021 tcaatcattt gcacatggat tggagtcaaa atttgatgca gtccgaacat aaccatcggc
    22081 aagaataatg tgcctagaac gaagccggca aatgctccac caacgccaag tacccagttg
    22141 atcccaccga ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa gaaaatttca
    22201 atcagaccga tggcaatcaa cgaaagagtt ggtgtaacga tgatatctac cgcatcaggt
    22261 actactttat gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa gatgacacct
    22321 aggatcccac cttgtccagc tgcaagagct tcaccagtaa ataagttttt cagtggtgct
    22381 tccggattca tccctgtcag catcacaacg gccccgataa ctccaccaag agttggtgtt
    22441 gcaccaaaaa cttgtgcagc attgatacct gtatagataa ctaggtagct aaacatcccg
    22501 tttttcaaga tgttcatcac atcgatatat tgttgccatg ttgctgcatc aagtgttccg
    22561 gctgtgacta agtttgctaa aactgcggca atccctgcca cgatcccagt accgacaaat
    22621 gcaggaatca ttggcacaaa gatattagaa atgtctttta ataccgattt gattttagat
    22681 ggtttttgtt tcgatttttg tgccgctttc atttcttgtg cccgttcgtt tacacggtct
    22741 tttccagatg ctgatgaatt tgctgtagct gtacttacag aagaaagttt ttctcctaat
    22801 ttcacgcctg ccaaatcaac catttgctgt gctactttgt tcactttccc aggtccgatg
    22861 atgacttgaa gctgttcatc ttctactaca ccaagcactc ctggaatcgc ttttaattca
    22921 gagacattta caagatcatc attgatgatc ttcatacgca cacgagtcat gcagtggaca
    22981 atgctttcta cattgcccat gccgcctgcc cctttataaa tttgttttgc cagttcttcc
    23041 aaagtcagtt cgttttttgc catggtcttt tccccctcat aaggtccctt ttacaaatcc
    23101 gtttgcttca gctaattttg cttgagcttc ctctttatca ctatttgtca aaatcatgac
    23161 gatagccagc ttaacatttt gatctgcttc ttcaaagtac ttttcagctg tttcgtaatc
    23221 agcttctgtc gcttgcatga tgattcgttt tgagcgttcg attagttttt catttgttgg
    23281 tttgacatcg accatcaagt tattgaatac tttacctata ccaatcatac tgatagtaga
    23341 aatcatattc aagataagtt tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa
    23401 aaattctgga cctgcatcga cttcgattgg gaaatccgcg tgtttactga tttcggcttg
    23461 tttattgcaa gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat ctaatccacc
    23521 gatcacatat ggtgtacgac cgctagctgc gataccaata actatatcat tttttgtcaa
    23581 attcagcttg attagatctt cttgccctag ctcataagaa tcttcggccc cttcgacagc
    23641 tacagtcatt gcttcttctc caccagcaat tagacctatg accatttccg gttctacacc
    23701 gaatgtcggt acgcattcag cagcatctaa cacacctagt cttccacttg ttccggctcc
    23761 catataaatc agacgtccac tttttttaaa ggactcaatc gtgttagcaa tcactggttc
    23821 gatacttgtc agttcttttt caaccgcagc ggccactttc tgatcttctt tgttcattag
    23881 ttgaacggct tgagaaatac tcatctcatc taatccaaaa gtttcttggt ttctgcgttc
    23941 cgttgttagg ttctctaaat tgattttgct catttttatc ttccttcttt tcttataaat
    24001 tgatagttca taccagcttt gatttggttg atcaatggta gctcttcttt gataacttta
    24061 gctactacat tcactttttc atccgctggc aaatcatatt tggttaattg gatctcaccc
    24121 atatagcgca aatacttttc gttatctaaa gtaatagacc ctttcatacg cgtagctgta
    24181 tttcttgctg ggataggcgg aatctctcga aaacgtgcat ctacgcttcg tatgacatct
    24241 cttgcatcgt cttgccgatt cgtatgcttc cctaatatat attcgtaaaa ttcttcatct
    24301 acaggttccg tatggagcaa gactgtctgt tctttttgga aagaagcaaa ttgctcctga
    24361 acgttttctg acagacctgc atctccgatg taaacaatat ccgtgttact tgctaataga
    24421 tctagagcgc ctgcaagagg atggatccct cgatgttctt ccaaagttgg caatccttga
    24481 tatagtgggc ctctcaactc tgtatctcct ggtacaaaac cttggacaag gagaccttga
    24541 attttgagcc attgattttt tttctgatac cattctttgt ccagccctgt ttcgggacgt
    24601 ggataataat tatgccaagc ttcaatctgt gagaaatcag cttctgcttc tttcaattca
    24661 tcgatatctt taggcgtgat cgtgctagca ttcaaactga ttttcaattg atgtgaccag
    24721 tcggcaatct gctgattgga aatatgataa tccattctta aacctgttac accaattgct
    24781 ttcaaaggcg ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc tcctgaaata
    24841 tcgaccatca attctagttt attctttttc gcttcgcttc ctagttcgat caatcgcttt
    24901 tgatatgccg aaacatcatc ttctggtata tgcatggaag tgaaaatacc gacaaagcca
    24961 ttggatgcca tttggcggat atactgtttc ttctcttctg acaaagaatc gttcataaat
    25021 attgaaaagc caaacatatt atccctcttt ttctgtatcc gtcttcattt ctatgactag
    25081 agtatatatc taaagaaaca aaatttcaat acttttatta aaataattaa atttcatttc
    25141 taaaggttga gtttatatta tcaaaagaat tgtcataacc taaaaaaggc actgctaagc
    25201 aaaagcagtg ccttgaaata ttttatttaa ataacacaaa aacataatta ctcataagaa
    25261 accaattctg tattatctac catgaatgta aagaacccct cattaggcat cggatgaaaa
    25321 taagcattga tcactcttat caaggcatta tggcaaacta acagatagac attttcttcg
    25381 tcttcgatgc attctttcaa taaaggaacg atccgtgcat acacatctaa gactgactcc
    25441 ccatttggga atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc atctttgctt
    25501 ggcagtccat cataatcccc aaaatccatt tctaccaatc gttcatcaac ggtcagcggc
    25561 aagctgagac gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg
    25621 atgattttcg tgattggaac aggcaaatct actacctttt ctgctagttt ctgtgcctgc
    25681 atctgtcctt tttcagtcag cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc
    25741 caagttgttt ctccgtgtct cgttacataa agcatgtgat tctctccttc gttggcaaag
    25801 ttccccttaa ccataccttg ttgttcttga gattacaagg gttagattct ctcaagagaa
    25861 ataatctcag tagatacttt atccaggaag gtttgatcat gttccactag taacatggca
    25921 ggtttcacgt tcaagatcag ttgttctaat tgttcttgat tgaagacatc caaataattc
    25981 aatggttcat cccatgtata tagttcagct ggctgtgata aagatttagc caattccact
    26041 tttttccgtt ggcccatact catctgctcg atcttgttat gaaaaacatc tctttccatc
    26101 ccaagctttc ggaggttgtt caaaaatgct tggtagtcta cttggttttt ctctgcaaat
    26161 tccgccaacg ttcctcgatt gtcttcataa ttttggcttg cataactaat gctcagatgt
    26221 tttggctggt atttttctcc tataaccttg ccgttgaatg cccccagaag ataatggata
    26281 atggatgact ttcctgcacc gttaggacct gaaatcgcca cccgctgatg aggctcgatt
    26341 gtaaaatgaa ttggctcgaa taacagattt tcatacccta attgaagatc ttctacgctt
    26401 aaaagtcgct tatggtgaga cgcttggcta ttcatcgtca aagaatcgat atattcgata
    26461 tcttttaata gtttttcttt ctcgctgatc tgggtctcca tccgattcac gattgctttg
    26521 gatcgtttca tcgtccgtgc agcatcagca cccactgctc ctttattcac tcgtctagat
    26581 tcagtatcga tgaatccgac ttgtttcttt gttttatctc cttctcggga acgagaccat
    26641 tcggcttttt cagctgctgt tttctttagc ctactgactt cttttttcaa tttttcattt
    26701 tgagccattt caaattcatc acgaagtttt ttctgttctt catagataga gaaattccct
    26761 tgataaagtt ccagttgact tttttcgatt gctaaaacat ggtccactac ttcatcgata
    26821 aatccccggt catggctgac cacgatgaag ccttgttttt tctttttcaa ataagccgct
    26881 acttgttttc taccagagat atccaaatga ttcgttggtt catcgattaa cgggaaatga
    26941 gtgtcatcca caaataaaag tgccagtagg acttttgtct tctctccccc cgatagtgtg
    27001 ctgaattctc tccataagat ttcaggatct gtttgcatca attggagctc tctttcgatt
    27061 tcccatatct caaaatccgt aatatcattt aacacgtaat aggttaaacg ttctttatct
    27121 tttgtctgtt gcgggaaata ggcaaattct tgctgatgga ttacttgccc ttgataaggt
    27181 agtttgtttt gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg tccgataagt
    27241 cctagtttcc attgtgtgtc aaaatttaga tttgcttgtt caaataataa tgtgccttgg
    27301 ctgtcgtagc cgaatgtcag attttttatt tcgattttag acattttcca tcacctcgta
    27361 aaaaaagacc agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggatacacac
    27421 aaaaagaccc atacatcgaa aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt
    27481 gtctccacaa ggtacagtct gaatgcagct ttcatcaatc gacttaattt ttcaatgcac
    27541 ggatccctca ctttgtccta tatttgaaat tacgatagca taaagaaaat gattttgtca
    27601 actacttcgg gaggatggtg aaaaatctgc taagtgtctg tcctaagatt tttgtttttt
    27661 ctaatgtttg cctgtgttcc attgcttctt ctaaacctat tggacccaaa tgaatactta
    27721 agacaattgg gagaaaatca gaaagtcctt ctagctcttg gtctctactt ccgcaaatag
    27781 cgattattgg ttttcgatat ttttttgcaa gcactgctac accgtatgga acttttccgt
    27841 ggatcgtttg ctgatcgatc cgcccttctc ctgtaatgat gacatctgct tgttcgatct
    27901 ttttttccaa tccagtgatt tggctgacta aatcgaaacc agaaaccatc ttcgctccca
    27961 acaaaaaaag ggctccacca ataccgcctg ctgctccact tcctggaata tcatctaaat
    28021 ccacctccca gaaatctttt atttgctgag caatctttcc tgcttgttta tctaagaaag
    28081 ctagcgtcgt atctattcct cctttttgtc ttccaaattg ccaagcagct ccttgttgac
    28141 ctgtatatgg attggtaaca tctgcagcga taatcaattt tactttagaa acaagaacgt
    28201 ttcttcgatc aaaatgaagt tttttagtag ataataaagg atttccttct gtgtaccctt
    28261 ctaaagaagc acccaaggat tggagtaatc ctagaccacc atcgctacac ccgcttcctc
    28321 ctaaagtgac aataatttgt tctacttctt gaagcagtgc atctttaata agttctccta
    28381 aggcataact ggaagcaagt cggatagttt gatccgttgg tttatcaagt agatctaacc
    28441 ccaatgcttg tgcagattcg atgactgcgg tgttttttcc ttgccaagtt gtcaataaat
    28501 agctgatttt tcgctgacgg aataagagat ccatcccatc aatggtacgc catgttcccc
    28561 tgagcgcgta atagattgct tctgcagtat tttctccgcc atctgcaatc ggcacattga
    28621 tttttcttcc tttacgatct tcccagccat ttagaactgc ctgatttagt tcaaatgaag
    28681 aagcacttcc tttaaatgaa tcaattgccg ccaaaatatc catctgtatc cctcactttc
    28741 ttcttaagta agacactttt tttcattttt ttcaaacaaa acaatgctat tttcttccat
    28801 ttttctatat actaaaaggg aactcattga ataaaggaga gaaaaaacat gaaaatcgca
    28861 atagctgggg ccggagcaat gggcagccga atcggattga tgctacatca aagcggaaat
    28921 gaggtcctct tgatcgatcg ttggcctgct cacattgaag ccattcgtac aaatgggttg
    28981 attgctgact ttaacggtaa agaagtggtc gcaaaactgc caatctattc accagaagaa
    29041 atcatcgaaa gcaacgaaca cgtggacttg atcgtagcat taacaaaagc caatcaatta
    29101 gatgatatgt tttgttcgat ccaatccatt attactgaca atacttatgt tttgtgttta
    29161 ctaaatggat taggtcatga agatgtactg gaaaaatatg tgccaaagaa aaatatctta
    29221 tttgggatca cgatgtggac agctggtcta gctggtcccg gaaaagtcac attgttaggt
    29281 gatggagaaa tcgaactaga gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt
    29341 gtagaagtct ttcaagaagc aaatttaaat ccgatctata gccataacgt tcgttattcc
    29401 atttggcgaa aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat tttggattgt
    29461 aatattgctg aactaggcgc gcaaaaagcg gcagagagca tggttcgaac aatcgtatct
    29521 gaatttgctt ctatcgcagc aaaagaagga atcatactag atcaagaaga agtgtaccaa
    29581 catattgctt ctacttatga tcctgacaac atcgggttgc attacccatc tatgtaccaa
    29641 gacctgatca aaaaccatcg tttgactgaa atcgactaca tcaatggtgc catttggcga
    29701 aaaggacaaa aatatgatat tgctacacca tattgtgcct ttttgaccca attagtccat
    29761 gcaaaagaag gaatccttgg cgcagaataa aaggctctct gatgagaatc agctgaatca
    29821 gcagctggtt ctcttttttt aagcccaata aaagatcaaa aagaaaacac aactctcatg
    29881 atgccataag aattgtgctt gtttgtttca gtacataatc tcttcaaaat gtaggttttt
    29941 tatttgtttt caacagagga gaggtcatat tcgtattgct cataaatagt ggaatcgtta
    30001 gtgataaatc gttttgccca attttctgca tcataaaatt ccaatagctc tgataggatt
    30061 ggcttaaatg gtgtatgcag aaattccggt aattcatcta aatcacgttc tcgaagcaaa
    30121 aaaatatttt caggacgata aaaatgataa tgagcaatcg cttgattatc cgtcactagt
    30181 ttcttttgaa aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc
    30241 aacagttcca gaatcccgcg cgattcccct gtttttttca ggtactcttc ataagtaaga
    30301 tatccaggtt gttcattccc tcgatgtgga agaatgaaat agttggtcgt aacatctctt
    30361 tttataaatt cctgcttata gctttctgct cgttcttttc tcccattatt cgttgcacca
    30421 aaaaatagat ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga ataaaatgaa
    30481 ctattatgtt tcagcccaag ttcttttgct tccaaaaaat caaaatgata aatctcatcg
    30541 attgccggtt catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc
    30601 caaaaaaata acacaatttt tgcattggta aatgggcgca cgcgtcgagc catctccata
    30661 tcaaatccat aatctaaaaa aacgatcagc tgataatttg ccaactgttg tctagctgat
    30721 ttaatgaatt ccgactgtag taaatctttt ccggaccaaa catctaaaga gcggatggaa
    30781 ggcaaaaaat aaagtgattt attttttgca actagcagta cgtcattttt gttcatcttt
    30841 tccgcctcac tttctctgtc actttttcct atgatagcct gtttaaatag taaaaagcca
    30901 gtaaaacgtt tcctatattt gtatgaaaat ttaacatttg tatgaaaata tgatgaaata
    30961 aatcattctt ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa
    31021 tttgttatca ttagtcattg ttaaatattg ctatttattc ttaatataaa ttttgattgt
    31081 gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc
    31141 agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt
    31201 ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat tggtgggctg
    31261 attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat
    31321 cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat
    31381 tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc
    31441 tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt
    31501 ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg
    31561 gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta tcttgtcggt
    31621 cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg
    31681 tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac
    31741 ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt
    31801 cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt
    31861 ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg
    31921 atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgtgattgtc
    31981 cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc
    32041 cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta
    32101 ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat
    32161 tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg
    32221 atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg
    32281 gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg
    32341 ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc
    32401 agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt
    32461 ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga caaaagtctt
    32521 gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa
    32581 accctctgtg caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat
    32641 gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt
    32701 ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt
    32761 cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat
    32821 gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc
    32881 tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga
    32941 cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg caagtaaacg
    33001 agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga
    33061 agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca
    33121 aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag aagctacttc
    33181 tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    33241 caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc
    33301 ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag tattagagag
    33361 acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc
    33421 tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa
    33481 aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac cgagaaactg
    33541 gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt
    33601 tttatgtt
//
LOCUS       DACBZN010000018        30887 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.018, whole genome shotgun sequence.
ACCESSION   DACBZN010000018 DACBZN010000000
VERSION     DACBZN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.018"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(27..725)
                     /locus_tag="IVD64_000885"
     CDS             complement(27..725)
                     /locus_tag="IVD64_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP6148156.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IVD64_000886"
     CDS             complement(718..2070)
                     /locus_tag="IVD64_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP6148157.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IVD64_000887"
     CDS             complement(2293..3693)
                     /locus_tag="IVD64_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP6148158.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IVD64_000888"
     CDS             3854..4591
                     /locus_tag="IVD64_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP6148159.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IVD64_000889"
     CDS             4716..5195
                     /locus_tag="IVD64_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP6148160.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IVD64_000890"
     CDS             5192..5959
                     /locus_tag="IVD64_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148161.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IVD64_000891"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IVD64_000891"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6148162.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDTITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IVD64_000892"
     CDS             complement(6862..7464)
                     /locus_tag="IVD64_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6148163.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IVD64_000893"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IVD64_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IVD64_000894"
     CDS             8145..8729
                     /locus_tag="IVD64_000894"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP6148164.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IVD64_000895"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IVD64_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP6148165.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IVD64_000896"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IVD64_000896"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP6148166.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IVD64_000897"
     CDS             10630..10809
                     /locus_tag="IVD64_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148167.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IVD64_000898"
     CDS             10831..11856
                     /locus_tag="IVD64_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP6148168.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IVD64_000899"
     CDS             12031..13275
                     /locus_tag="IVD64_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP6148169.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IVD64_000900"
     CDS             13428..14375
                     /locus_tag="IVD64_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6148170.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IVD64_000901"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IVD64_000901"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP6148171.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPGGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IVD64_000902"
     CDS             complement(15277..15519)
                     /locus_tag="IVD64_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148172.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IVD64_000903"
     tRNA            15640..15712
                     /locus_tag="IVD64_000903"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IVD64_000904"
     CDS             complement(15831..16469)
                     /locus_tag="IVD64_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6148173.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IVD64_000905"
     CDS             complement(16592..17224)
                     /locus_tag="IVD64_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6148174.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IVD64_000906"
     CDS             17575..17874
                     /locus_tag="IVD64_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6148175.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="IVD64_000907"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="IVD64_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP6148176.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="IVD64_000908"
     CDS             complement(20806..21291)
                     /locus_tag="IVD64_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP6148177.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21771..22412
                     /locus_tag="IVD64_000909"
     CDS             21771..22412
                     /locus_tag="IVD64_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6148178.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="IVD64_000910"
     CDS             22431..23066
                     /locus_tag="IVD64_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6148179.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="IVD64_000911"
     CDS             23084..23899
                     /locus_tag="IVD64_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6148180.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24218..25654
                     /locus_tag="IVD64_000912"
     CDS             24218..25654
                     /locus_tag="IVD64_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6148181.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25843..28290
                     /locus_tag="IVD64_000913"
     CDS             25843..28290
                     /locus_tag="IVD64_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6148182.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28520..29587
                     /gene="rlmN"
                     /locus_tag="IVD64_000914"
     CDS             28520..29587
                     /gene="rlmN"
                     /locus_tag="IVD64_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6148183.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29780..30883)
                     /locus_tag="IVD64_000915"
     CDS             complement(29780..30883)
                     /locus_tag="IVD64_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6148184.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT        10591 a         5289 c         6283 g         8724 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atactattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gggggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
    24061 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
    24121 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
    24181 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
    24241 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
    24301 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
    24361 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
    24421 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
    24481 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
    24541 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
    24601 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
    24661 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
    24721 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
    24781 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
    24841 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
    24901 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
    24961 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
    25021 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
    25081 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
    25141 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
    25201 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
    25261 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
    25321 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
    25381 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
    25441 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
    25501 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
    25561 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
    25621 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
    25681 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
    25741 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
    25801 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
    25861 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
    25921 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
    25981 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
    26041 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
    26101 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
    26161 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
    26221 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
    26281 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
    26341 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
    26401 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
    26461 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
    26521 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
    26581 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
    26641 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
    26701 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
    26761 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
    26821 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
    26881 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
    26941 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
    27001 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
    27061 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
    27121 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
    27181 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
    27241 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
    27301 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
    27361 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
    27421 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
    27481 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
    27541 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
    27601 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
    27661 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
    27721 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
    27781 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
    27841 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
    27901 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
    27961 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
    28021 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
    28081 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
    28141 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
    28201 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
    28261 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
    28321 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
    28381 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
    28441 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
    28501 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
    28561 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
    28621 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
    28681 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
    28741 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
    28801 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
    28861 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
    28921 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
    28981 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
    29041 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
    29101 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
    29161 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
    29221 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
    29281 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
    29341 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
    29401 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
    29461 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
    29521 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
    29581 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
    29641 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
    29701 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
    29761 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
    29821 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
    29881 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
    29941 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
    30001 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
    30061 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
    30121 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
    30181 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
    30241 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
    30301 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
    30361 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
    30421 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
    30481 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
    30541 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    30601 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    30661 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    30721 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    30781 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    30841 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattc
//
LOCUS       DACBZN010000019        30506 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.019, whole genome shotgun sequence.
ACCESSION   DACBZN010000019 DACBZN010000000
VERSION     DACBZN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.019"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IVD64_000916"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IVD64_000916"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP6148185.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2423..3118)
                     /locus_tag="IVD64_000917"
     CDS             complement(2423..3118)
                     /locus_tag="IVD64_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP6148186.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IVD64_000918"
     CDS             complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IVD64_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP6148187.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(4317..4508)
                     /locus_tag="IVD64_000919"
     CDS             complement(4317..4508)
                     /locus_tag="IVD64_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP6148188.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4529..5419)
                     /locus_tag="IVD64_000920"
     CDS             complement(4529..5419)
                     /locus_tag="IVD64_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP6148189.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5406..6173)
                     /locus_tag="IVD64_000921"
     CDS             complement(5406..6173)
                     /locus_tag="IVD64_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6148190.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IVD64_000922"
     CDS             complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IVD64_000922"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP6148191.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IVD64_000923"
     CDS             complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IVD64_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP6148192.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IVD64_000924"
     CDS             complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IVD64_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP6148193.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10789..11127
                     /locus_tag="IVD64_000925"
     CDS             10789..11127
                     /locus_tag="IVD64_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148194.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(11295..12053)
                     /locus_tag="IVD64_000926"
     CDS             complement(11295..12053)
                     /locus_tag="IVD64_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6148195.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(12071..12889)
                     /locus_tag="IVD64_000927"
     CDS             complement(12071..12889)
                     /locus_tag="IVD64_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6148196.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IVD64_000928"
     CDS             complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IVD64_000928"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP6148197.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IVD64_000929"
     CDS             complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IVD64_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP6148198.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            14017..15351
                     /gene="dnaA"
                     /locus_tag="IVD64_000930"
     CDS             14017..15351
                     /gene="dnaA"
                     /locus_tag="IVD64_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP6148199.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15549..16679
                     /gene="dnaN"
                     /locus_tag="IVD64_000931"
     CDS             15549..16679
                     /gene="dnaN"
                     /locus_tag="IVD64_000931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP6148200.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            16908..17150
                     /gene="yaaA"
                     /locus_tag="IVD64_000932"
     CDS             16908..17150
                     /gene="yaaA"
                     /locus_tag="IVD64_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP6148201.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            17137..18261
                     /gene="recF"
                     /locus_tag="IVD64_000933"
     CDS             17137..18261
                     /gene="recF"
                     /locus_tag="IVD64_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP6148202.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            18258..20204
                     /gene="gyrB"
                     /locus_tag="IVD64_000934"
     CDS             18258..20204
                     /gene="gyrB"
                     /locus_tag="IVD64_000934"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP6148203.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            20224..22695
                     /gene="gyrA"
                     /locus_tag="IVD64_000935"
     CDS             20224..22695
                     /gene="gyrA"
                     /locus_tag="IVD64_000935"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP6148204.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            22931..23230
                     /locus_tag="IVD64_000936"
     CDS             22931..23230
                     /locus_tag="IVD64_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP6148205.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            23280..23792
                     /gene="ssb"
                     /locus_tag="IVD64_000937"
     CDS             23280..23792
                     /gene="ssb"
                     /locus_tag="IVD64_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6148206.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            23818..24054
                     /gene="rpsR"
                     /locus_tag="IVD64_000938"
     CDS             23818..24054
                     /gene="rpsR"
                     /locus_tag="IVD64_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP6148207.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            24197..26170
                     /locus_tag="IVD64_000939"
     CDS             24197..26170
                     /locus_tag="IVD64_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP6148208.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            26176..26628
                     /gene="rplI"
                     /locus_tag="IVD64_000940"
     CDS             26176..26628
                     /gene="rplI"
                     /locus_tag="IVD64_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP6148209.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            26902..28269
                     /gene="dnaB"
                     /locus_tag="IVD64_000941"
     CDS             26902..28269
                     /gene="dnaB"
                     /locus_tag="IVD64_000941"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP6148210.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            29049..30341
                     /locus_tag="IVD64_000942"
     CDS             29049..30341
                     /locus_tag="IVD64_000942"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP6148211.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         9213 a         6023 c         6024 g         9246 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa
     2221 attttgtgaa aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg
     2281 cttttgaaca ccgtttatcg taaaaaggga ctgtgacaag acttttgtca cagtccctta
     2341 ctattattta actatggata aacaaaatga ggttctttac tacccctaga tatctagcaa
     2401 aatagctgat ctttttgcta gtttatcttc ttgggacgga taaacttcca ataatcccgt
     2461 attttttctt caagtagtaa cggattgcta aagcagttgc cccgataatg ataactacta
     2521 ctggatctaa tactggattg ataactggtg gcaatagtgt tgtcgcagaa aacagtacga
     2581 cccaaagaag tgtcgttcca cctaggataa gcaatgtctt acccattcct ggtttcttgc
     2641 ttcgatctgc tccgggatat tcatattgat agatgtattt gtacatcata taaaagaccc
     2701 agccaccgat tgcagaacct agaacgagtg tagaaaagcc ataagctggc gttgttccac
     2761 gtgaaaacaa tccggtaatc gcaatcatga tacttaatag accaagcaac agcaacgtat
     2821 tatctaacca catcaataaa ggggtggatg aattttttac ttctgctggc ttattgatga
     2881 ttgcttccgt tctttcagag actgtcccaa acaattgacg ggctgttctt ccgccttttt
     2941 gttctgcaac taaagtaggc agaatgtcat gaagggcttt tactttttct tcttcagaca
     3001 aattcgctgc ttctaaagat tttttcaaat caaaaatgta ctgttgattt cttttggtta
     3061 attttgtttc taaactattg ttttctttta cgattgctcg taattgttct ggttccatta
     3121 tctaatagtc ctccttagcc gttccttttc tcaacggtcg tcacaaaaga cattctacca
     3181 tattctacag ctagtttgat agccctttta cacattaaag cggaataaca ttacgtcgcc
     3241 gtcttgtacg acataatctt ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg
     3301 catgtttccg taatgatcca agtcttcaaa agagacagtt tctgcacgga taaatccgcg
     3361 ttcgaaatct gaatggatga ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt
     3421 ccaagcacgt acttcttgtt ccccagcagt aaagtaagta gctaagccta acaaatcata
     3481 agcagcacgg atcaactgat ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc
     3541 cgctttgtct tcttcatcta attctgcgat ttcttcttct gcacgagcac tgaccacgat
     3601 cacttctgca ttttcttctg cagcaaagtt gcgcacttct tgtacataat gattcgcgtc
     3661 tgcatctgct acgtcatctt ctgagacatt ggctacataa agaattggtt ttgtcgtcaa
     3721 taaaaatagt gatttaacga ttttttgttc ttcttcagta aattcgatcg ttcgggcaga
     3781 ttttccttct tccagtacag gtttgatttt atccagtaca gctagttctg ccactgcatc
     3841 tttatctttg gttttagcaa cttttgctac acgagtatag cgtttgttga tagattctaa
     3901 gtctgctaac actagttcca agttgatcgt atcaatatct tctagtggat caacacgtcc
     3961 ttctacgtga gtaatattat catcatcaaa acagcgaaca acgtgacaaa tagcgtctac
     4021 ttggcggata tggcttaaga attgatttcc caaaccttct cctttacttg cgccttttac
     4081 gattccagca atatctgtaa attcaaaagt cgtcggaaca gtttttttcg gatgtacaag
     4141 ttctgttaag cgttgcaaac gccagtcagg tacttctacc atccctacgt tggggtcgat
     4201 cgtcgcaaag ggatagtttg ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt
     4261 tgattttcct acgttgggca acccaacaat tccagcagtc aatgccataa atttattcac
     4321 tctttctctg catttttttc aattactttt tttaatttct tttcaaaatc tcgacgggtc
     4381 atcatgacga tatgtccgca attcatacat ctgattttga tatctgcgcc cattcgaatg
     4441 acttcccagc gattcgtctg gcaagcgtga ggttttttca tttcaacgat atcgtgcaag
     4501 tcgtacataa aaatagcctc ctttttgttt attcttcatc aaattgaata ttcaagatat
     4561 caagaattcg agtcaaatct ttttgagaaa gatattcaat ctcgatctta cctttcccat
     4621 ttttttcttg gatagctaca cttgtaccaa atttatccat caaacgatct tcgctttctc
     4681 ggatataata tggtttttcc tttgctagcc ttgggatttt tttcttttct tttccatcat
     4741 tttgatttaa ctcagaaaca agctgttcca gttggcggac agttaaattt tcagttactg
     4801 cacgattggc taatttcaag atcaatgttt tattttttaa acctaacagc gtacgggctt
     4861 gtcccataga taaacgctga tcttgtacca tttccttgac aagatccggt aaagaaagca
     4921 agcgcaaata attggcgata taaggtctgc tctttcctaa acgactagct acttctgctt
     4981 gtgttagttt caggtttttc atcagcatct catatgcttc tgcttcttct aacggattca
     5041 gatcttctcg ctgcaagttt tccagcactg ctacttgcat catcgcttct tcgtcaaagt
     5101 ctctaacgat agcagggatc gtttcttttt cagctagttt ggacgctcga aaacgtcgct
     5161 cccctgcaat aatctcatat ccttttactg cggaacggcg tacgatgatg ggttgaaaga
     5221 cacctgattg ttcaatagaa ttagctagtt cctgcaaaga tacttcttca aatgttttac
     5281 ggggctgata cggatttggt cgcagctcat tcaatggtag ctggacaacg gtatcatctt
     5341 ttacatccac cgtctctaag ttttctaaat cttgaaacag tgcatcgatt cctcggccta
     5401 atcctttact tttcttcacg acttaccact tcctttgcca atgcttggta gacttcagct
     5461 cctcgagaac gtggatcgta gtcgatgatc ggttttccat gactaggtgc ttctgataaa
     5521 cgaatatttc ttggaatgat cgtttcgtaa actttttcgc ggaagtattt acgtacttct
     5581 tctacgactt catttcccag atttgttcgt gcatcataca ttgtcagtaa gactccctcg
     5641 atctctaatt ctggattaaa gtgtttttgg actaaacgaa ccgtattcag taattggctt
     5701 aatccttcca atgcgtaata ctcacactga acaggtatca aaatcgaatc acttgctgta
     5761 aatgaattga ttgtcagatg gccaagagaa ggcgggcaat cgatcaaaat ataatcatac
     5821 tgactgctta cttctgctaa agatcctttt aatcttgatt ctcttgccat cattgacgtc
     5881 aattcgatct ctgctcctgc taattggaga gtcgcaggca caatactcaa attttcgtgt
     5941 tcagtgatca aagttgcctc atcaattggc aattcattga ctagaacatc atagatgtct
     6001 cttgtcacat caggttttct gatcccgaca ccactggtag catttccttg tgcatccata
     6061 tccactagca atactttttt gccaaggcta gcaagacaag cacctagatt caccgtcgtc
     6121 gttgttttgc cgacgccacc tttttggttc gcaacagata ttatttgtgc catctcttct
     6181 cctcctattt gattggctgc ttattaggca gtccgggctt tcttgggtat ttctttgggg
     6241 ttggtttctt tttttgaata acgatgatat gccgttgatc agagacattc ggcaaggtaa
     6301 ccgcctcttc tttgaggaat ttaccaccta ataaagcaat cgcttgtttt gcttctgtca
     6361 attcctcttc actttttgct gctttcaatg cataaaaata gccttcttgt ttagccaagg
     6421 gcaaacacaa ttccgctaac acattcaaac gagcaaccgc acgagcagtg acaaggtcaa
     6481 atgatgcacg aaagtgagga ttttgcccaa atgtttctgc ccggtcatga tataaggaag
     6541 catcgattcc taataatcgg gttaactcag ataagaaggt tattcgttta ttcagagaat
     6601 caacgatcgt tatctgcagt tctggaaagg caatttttaa aggtatgcta ggaaaccctg
     6661 cccccgaacc aacatcgcat aaagataaag gttgcgtaaa gtctacttca aatgctaatg
     6721 taagtgaatc gtaaaaatgc ttcagatata cgtctttttc atccgtaata gctgtaaggt
     6781 tcatcttttc gttccattcg actaatgtcc gataataaat agcgaattga ttcatttgat
     6841 gatcggtaag ctctatccct ttttctgcca aggattggcg aaattcttct ggtatcataa
     6901 atattccttt cattgtgaaa cagcaattgt ttcacagatt ctctccctac tttaacgcaa
     6961 aatctctaga catgcaatcc ttccatatgg attcttaaaa atttgaaaag taagaaaagc
     7021 cgatagactt tttctttcta gtatgactca taaaatatac acagaagaga gaactagctt
     7081 tgagcaattt ctgcaaaaac tagttctctc ttctatttat tattacgtat tacgctggtt
     7141 cactgacttt cgcaatcttc ccttgttcga tatagaccat taggatactt aggtctgcag
     7201 ggtttacgcc actgattcgg ctagcttggg caattgtttc tggttggatt ttctgcaatt
     7261 tttgttttgc ttctgttgct aatccgttga ttgctgcgta gtcgatgttt tctggaatct
     7321 ttttcgcttc catgcgtttt aatttttcta ctttttccat ggcttttttg atgtagcctt
     7381 catatttgat ttggatttct acttgttcga tttcttttcg tgtcaattct tcattttctg
     7441 gaataaaccg tagaagttct gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta
     7501 gtacaccatc ttttaatggt gccgcattaa tcgttgctaa aaagtcttgg acttcttctg
     7561 ttggtttgat tcgtacattc atcaaacgtt tgatttcttg ttcaactgct tcttttttct
     7621 tcaaataatt cttgtattga tcttctttta ctaagcctac acggtgaccc atttctgtta
     7681 atcgcaaatc tgcattatca tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca
     7741 aacgataagg ttcgttcgtt ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac
     7801 cgtcacttcg tttgatcact aaaggttctt ttccctgaac tttcaaagct gcattgatac
     7861 cggcaatcag cccttgtcca gccgcttctt cataaccgct tgtaccattt gtttgtccag
     7921 ctgtataaag attttcaacg actttagttt ctaatgtcgg acggagctgg tgtggctcta
     7981 ctacgtcata ttcgatagca tatcctgtac gcatcatttc tactttttct aatccagcaa
     8041 tagaatgaag catgttgact tgaacatcct ctggtaaaga agtagataaa ccttgaacat
     8101 agacttcttc cgtatttaag ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg
     8161 caaaacgcac gatcttatct tcgatggatg gacaatagcg agcgcctacc ccttcaacga
     8221 tccctgtaaa cattggggca cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac
     8281 ctgtataggt cagccagcaa gattcctgat ctagtttata ggcactatct ggtgtgctga
     8341 agctaaaatg attcggctct ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg
     8401 tacttgattt tactcgaggc ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt
     8461 cttttagatg attcgctagt ccaatagaag gttgagagtt attcggacct gatgaatatt
     8521 tcaattcacc gataatgatt tctccgcgaa gtgctgttcc ggctgtaata acaactgctt
     8581 tggcccggta gctcgctcct gtggacgtca ctactccatg acaaacacca tcttcgacga
     8641 tcagttcttc gacgattcct tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt
     8701 tcatttctgt tgaataagca tgtttgtctg cttgtgctcg caatgctcgg acagctgggc
     8761 cttttcctgt gttcaacata cgcatttgga tataggtttt atcgatattc ttacccattt
     8821 ctccgcctaa cgcatcgatt tctcgaacga caacgccttt agctggtccg cctacagaag
     8881 gattacatgg catgaatgct accatgtcca aatttattgt cagtaataat gtttttgatc
     8941 ccatacgtgc agcagcaagt gcagcttctg aaccggcgtg tcctgcgcca acaacgatca
     9001 cgtcatacgc atctgcttca taatgttcca atcgtttgtt cctcctttta ttttcctaaa
     9061 caaaattgac tgaataattg agtgattagc tcatcttgta cactgtcgcc gacaacttct
     9121 cctagatagt cccagcaacg cgtcatatct atttgaacga gatccacagg cattcctgaa
     9181 tcaatacctg caatgacttc ttcaagtgag agggaagctt tttctaacaa cgcgatatgg
     9241 cgggtattag aaacataagt agcgtctctt tctcctgttt ctccgctgaa gaataaatca
     9301 gaaatagctg attccaaacg atctagtccg tcgtttttag ctacggaaat tgaaaaaact
     9361 ggttcatttt cgatcaattt cttcaatttt tcttgttcca gctgtgctgg caaatctgtt
     9421 ttatttagaa gaatgatccg tttcaatcca cttgtcgctt ccaataattg ttcatcttct
     9481 gctgtcagtg gttcgctttg gtttagaaca agtaagatca agtctgcttc tgctaatgct
     9541 tttcggcttc tttcgacccc gatccgttcc accacatctt ctgtttcccg aattccagct
     9601 gtatcgatta gcttgagcgg taccccccga acattgacat actcttcgat tacatctcga
     9661 gtcgttcctg caatatccgt aacgattgct ttttcttctc ttaacaaatg gtttaacaaa
     9721 cttgatttgc cgacatttgg ccggccgatg attgccgtac ttaacccttc ccgcagaacc
     9781 ttcccttgct tggatgtagc aagtagcgct tggattcttt gttggatcat ctgcgctttt
     9841 tcaagcaata acttcgtagt caattcttcg acatcatcat attctggata atcaatattt
     9901 acttctactt gtgctaatgt ctctaaaatc tcttgtctca atgagcggat caaagcagac
     9961 agattgccgt ctaactgatt aagagctagt cccattgctt tgtctgtttt tgcccgaatc
    10021 aaatccatca cagcttctgc ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt
    10081 gtgaattcgc ctggctcagc taatcttgca ccttctctta acaatagctg caaaatttga
    10141 ttgactacaa cgatcccacc gtgacaattg atttctacta catcttcccg cgtaaatgtc
    10201 tttggtgctc tcatcacgga gaccatgact tcatcgacaa gctgattgct cttgggatca
    10261 acgatatgtc cataatggat cgtatgactt ggtacttctg ataatcgttt gtttccagat
    10321 tgataaactt tatctgctag ttgcacagct tgatcgccac ttaaacgaac gatactgatt
    10381 gcaccttctc caggtggggt agaaattgcc gcaatcgtat caaattctaa agtaatttca
    10441 gccatttttt cacttccttt ttcattttgc gcacaaaaaa agtgcccact ccacccttta
    10501 tgcacaataa acggtgaact tcgcactttg actgcttgtt cttgattaaa ttctggtcat
    10561 agattatcac acttgctata aggaatcaag tgtttaaaac aattagatga agttgagcct
    10621 gttctgcttc cattacctta tatttaattg tccttggatg tctctttgcg ttctttggtt
    10681 tattcgaaga gaagatactg tataaacaag gctttatctt tcttaaaaaa aacggtacac
    10741 taaatggtag cgctatctaa actatacact tttgaaagga gaaaatatgt gagcccgttc
    10801 agatctgctg cattggaaga agaaagtgac cctcggatca acgatgtcgt tttatctaca
    10861 gggaatatca accagaaata tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa
    10921 gctgatttgt ttgacccatc ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa
    10981 ttaaagacga aggtacttga atatggtgcg aatgcagtaa tcaactgtca ttttgagtat
    11041 cagcaaaaaa aacgcgacgg aaagtatttt ctggaacttt ttgcttatgg aacagtgatc
    11101 caatttatcc aaacaacgat tggttaaatg aaacagacaa aaaaagcgga tcaaaactga
    11161 agacagtttc gatccgcttt tttctatcaa agttagtgac tacttcatcg tatactctga
    11221 taagaatgat ttctagtaaa atatacttgg tttgaaaaac tttgtcaacg tgatttattc
    11281 aaaaagtaat ggaatcaata atatttttta gcaggttcta ctactaaata acgataaggt
    11341 tcctcacctt ccgaatgtgt ttttacatgt tcatctttgc ttaatgcagc atggatttgt
    11401 ttacgttcaa atgcaggcat tggttctaag aaaacaggct gccctgtccg ttttactttt
    11461 tcagccgtgc gatcagctaa acgctgaatg atctcttggc gcttttcacg gtaattgcct
    11521 acattgacta ctacagacag cttatttctt gctaaacgat ggatgaagac ttgtgccaga
    11581 tattgcaatg cattcagtgt ttttccatgt ttcccaatca gtatgccttg cttttgtgtt
    11641 tctaaatgga aaacaaccat attatttgat tctctttcca ttttgacaag agctggagca
    11701 tcaagctgtt ttgaaatttc agtaagataa actgccaatt ccgttaaagc tgcttcatca
    11761 cctagctcac ttacttcttc tgttttttct tcaagaatcg tttcgttgac tacctcgatc
    11821 tcagctgttg gttcttcttc caaaatttct tctacagtct gttctactgt ttcagaaact
    11881 ttttcgctga tacttggctg gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa
    11941 cctttttttc cttcttccag tatttcgatt tcgacttggt ctttggccaa acccaaatca
    12001 gccagaccta gttggacagc ttcgtctatt gttgcagctt cataaatcgg cattagcatt
    12061 acctccccta ttatttccgt tttttcttag gattcattgc tttcttaaga gcacgttctc
    12121 tttcgcggat tctccgtgct tcttcttcac gctcttttcg aattttgaaa gggttgttga
    12181 tcagcagcgt ttgtacaact tggaaggcat tggaaactac ccagtataat gaaagaccgc
    12241 tggctaaatt gacacccatc actaaaatca ttacaggcat cacgaaattc atgatcttca
    12301 ttgatgcatt cgattcaatt tgactcatgc tagataaata cgtacttgca aaagtaaaga
    12361 ttgctgctaa aatcggcaaa ataaatgttg gatccgcgtt ccctaagttc aaccataaga
    12421 aatgcccttg tttcaactct ggtacacgtg aaatagattg ccataacgcc atcaagattg
    12481 gcatttgtac taataaagga agacaaccag catatggatt gacgccattt tctgcatata
    12541 aacgctgctg ctcttctctt aacttacttt gtgtttccgg atctcttgaa ctatattgtt
    12601 gctgcagtgc ttttaatttc ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga
    12661 aatgcatcaa cggcaatagg atcacgcgga tgatcagtgt aaataaaatg atccctatcc
    12721 ctgcattacc aaatgacaac gccttgatcg cctcagcaaa gtaataaaca atataacgat
    12781 cccaaattcc tgtactctgt gcgctgactt ccccagttcc gcaaccggat aagacgatga
    12841 ccaaactaac gataccggcc atcaaaagta aacgtttgta tttcttcact tactcttttc
    12901 ctctctgtcg attatttttg caagttttaa gacgtggact aaattggagc gaacctcatc
    12961 ataagtcaac cccttcacac ttggtctagc aatcacaata aaatccaact ccggatcaat
    13021 ttcttctttt atagaataaa ctgctgctcg gatttttcgt ttgacttcat tgcggtttac
    13081 cgcgttgccg accttttttc ctacagataa accaactcga aaatgaggct gtcccggttt
    13141 ttccaaacgg taaacgacga atttgcggtt agcaaaagaa tctcccgtat ggaacacggc
    13201 ttgaaactct cgttcttttt ttactcgata cgcttttctc attagttttc tttccttttc
    13261 tatctctcac ttacgctcat aatcatacca taattacgat tgtcctttca tcatttccct
    13321 aaaaaagagt ataaaaaaaa ccactgaaac cccagtggcc taagctgaaa gaacttttct
    13381 tcctttgcga cgacggctag ctaacacacg acgtccattt ttagtactca tacgtttacg
    13441 gaatccgtga actttttgac gtttacgttt atttggttgg tatgttcttt tcatatattc
    13501 cacctccaaa atgttcttat ctattttata aacacagaca tactttgata gtataacgac
    13561 aaagtagtga ttttgtcaat ataaaataga agatcgattg ttaaaactta tccacaagat
    13621 tatttttttc tatgggaatc ctatgtggaa aaagttatgc acagggatta tccacagata
    13681 ataaaaggct ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg
    13741 atttcctgtg tattccgtct cctaactatt ggtatatctg tattttttat ccacagacgc
    13801 aagaggaaat ctattggttt ttacgagttt tccacctgtg cattagtggt gtggaaaact
    13861 ttttttgaca actgtgtttt tctctttttt ctttttgtcg attgtgtgga taattgtaat
    13921 taaaaaattt ttttcttcgg tttctgtgga aaactattga aaaaaatgct aaattagtac
    13981 tagttattca ctttttaatg aaggaggagg ccttatatgg tatccctcga tgctttatgg
    14041 aatgaattaa aagcaacata ccaaaaggat ttatcgcctg ctagctataa tacatggatc
    14101 gaaacagcga accctcgaac ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag
    14161 attcataaag aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg
    14221 ttatcaggaa atgagattat ccctcgcttt attactggcg aagaagcaga gcaagaagaa
    14281 gttatagaag aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg
    14341 aaccctaaat atacctttga tacgtttgtc attggtaaag gaaaccagat ggctcatgca
    14401 gcagcgctcg ttgtagcaga agatccaggt tctatttata acccgctctt cttttatgga
    14461 ggcgtgggac ttggtaagac tcacttgatg catgcaattg gtcatcaaat gctgcaaagt
    14521 cagcctaatg ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga tttcatcaat
    14581 tcgatccaaa acaaaacagc agaagagttc cgtcaagaat atcgaaatgt tgatttactg
    14641 ctagtggatg acattcaatt ttttgcagaa aaagaagcta cgcaagaaga gtttttccat
    14701 actttcaatg ccttgtacaa tgaagggaaa caaatcgtac taaccagtga tcggttgcca
    14761 aacgagattc cgaaactgca agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt
    14821 gatattacac cgccggatct tgaaacaaga acagcgattt tacgtaaaaa agcggcggct
    14881 gaacgtctag agatcccaga tgacacacta agctacatcg caggacaaat tgattctaac
    14941 attcgtgaat tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa
    15001 gatatttcga ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat
    15061 cctcaattgt caatcctgca gatccaagaa gaagtggcaa agtattatca catccaattg
    15121 aaagatctca aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca aatcgctatg
    15181 tatttggcac gtgagctcac agataattct ttaccgaaaa tcggagctga attcggtggc
    15241 aaggatcata caacggttat ccatgctcat gaaaaaatcc agcagctgat ggattctagt
    15301 gtcagtatgc aaaatgaagt atcagaaatc aaaaactttt tactaaatta atttgtcgat
    15361 tctatcaact gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt tatacacact
    15421 ttttccacag ctgttttttc gtttctctct tacgtttttt gagttttcca cagaatcaac
    15481 atgccctatt acttttatta ttctttttaa ctaataataa ataaacaaac attcaaagga
    15541 gatacatcat gaaagttact ttaaaccgag ctagctttat gcaggaattg caaactgttc
    15601 aacgagctat ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa atcacactga
    15661 cacaagaagg tttgactttg acggggagca acgctgatat atcaattgaa acttttttgt
    15721 ctgttgaaaa cgaaaaagca aatatgcaaa tcgaatctac tggttccatt gttttacaag
    15781 cacgtttctt tagcgaaatc attcggagac ttcctgaaga aacatttact ttagaagttt
    15841 tagaaaataa acaagtagcg atcacttctg gaaaagcgaa ttttatcgta aatggattag
    15901 atgcagataa ctatcctcat cttcctgttg tcgaaagcca taaccagatg aaattacctg
    15961 tacacgtatt gactaaacta atcaacgaaa cagtttttgc tgtctcacaa catgagagtc
    16021 gtccaatctt gacaggtgtg catttcattt tatctgataa ttctttatta gctgtagcta
    16081 ctgattctca ccgtctaagt caacgcgtga ttccagtaga acaagcggct gatcattttg
    16141 atattgttat tcctggaaaa agtttgatcg aattatctcg ctcattaaca aatgaagaag
    16201 aaatcgtcga aatcagcatt atggaaaacc aagtgttgtt caaaacagaa acgatgtatt
    16261 tctattcccg tttgctagaa ggaaactatc cagataccaa tcgtttgatt ccttcaagtt
    16321 ttaatacaga ggttgaattt tctgttccta gctttttagc ggcgatcgaa cgggcttctt
    16381 tactttctca tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca gatgcggttg
    16441 ttttatacgg aaattcgcca gaaatcggga aagtagaaga aagcttgagt tatacagcaa
    16501 gcagtggcga tccattagat atttctttca atccggatta tatgaaagca gcgttgcgtg
    16561 catttggaga tatgagcatc aaagtgaaat ttatctctgc tattcgtcca tttacattgg
    16621 aaccaacgga agatggcgtt caatttattc agctgattac tcctgtacgt acaaattaac
    16681 aatgattttc aatgttcttg aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa
    16741 acagaaggcc ctaaaatctc ttttaaggct ttctgtttat ttttgtttaa atcacctttt
    16801 ttagattaaa tcgtcttata tggaaaaata tatggataaa tttgttttat tgcgaaaaaa
    16861 aaggtataat agagtatact tttaataatg aaaatgaggg atgggcattg aaacaacaag
    16921 tcattctaaa tacggaattc atgacgttgg gccaaatgtt gaaagaagtc acagttatcg
    16981 gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc gatggcgaac
    17041 cagaaaatcg tcgtggacgc aaactttatc ccggcatgat ggttgaagtt ccagaagtag
    17101 gaactttttt tatggtaaaa aaaggaaacg acgataatga ggctgaataa gctgtattta
    17161 aaaaattatc gaaattacga agagctgaac atcgaatttt ccaagaacct cgtcatcttt
    17221 ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt attagctatg
    17281 acgcgaagtc atcggacaag tagtgagcag gaattgattg gctggactga cgatcaggcg
    17341 atgattcaag gagagatcac aaaaggttca tccactcttc cattagaaat cttattatct
    17401 aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaga aaaagttaag tacttacgtg
    17461 ggacaaatga atgtcatttt atttgcacca gaagatcttt ctttagtaaa aggcagcccg
    17521 cagaccaggc ggaagttttt agatatggaa ttaggccaga tcaatccaat ctatctttat
    17581 gatcttgtcc aataccaagc aattttgaaa caaagaaacc agtacttgaa gcaattgaat
    17641 gaaaaaaaac aaactgattt gctttatttg gatattttgt ccgaacaact agcagcgttt
    17701 ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc gattggagtt ttgggctaat
    17761 tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca
    17821 gtcgattctt tagaaacaca tacgcaagag caaatccaag aacaatttct cgcacttttg
    17881 aaccaaaata aaaagaaaga cctttttcgg ggaacaacga cagttggtcc tcatcgtgac
    17941 gatctttcct tttttattaa tcagaaaaat gtgcagactt atggatcgca aggacagcaa
    18001 agaacaactg cactaagtgt caaattagca gaaatcgatt tgattaaaga agagacaggg
    18061 gaatatccaa tccttttgtt agatgatgtg atgagcgagc tggatgataa tcgtcaactc
    18121 catctgctag aaacaatcga aggaaaagtt caaacgttct taacaacaac gacacttgac
    18181 catgtaaaaa ataaaatgac tgttgaacct gagatttttt atgttcaaca agggaaacta
    18241 aaaggagaag aaactgaatg acagaagaaa gaagtttagt agaacgcgct aaagagtatg
    18301 atgccagtca gattcaagta cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt
    18361 atatcggttc gactagttct gagggtctgc atcaccttgt ttgggaaatt gtcgataatt
    18421 cgatcgatga agtattagca ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca
    18481 acagtatcac cgttattgac gacggtcggg ggatacctgt agatatccag gcaaaaactg
    18541 gacgtcctgc agttgaaacg gtctttactg ttttacatgc tggaggaaaa ttcggcggtg
    18601 gtgggtacaa agtttctggt ggacttcacg gagtaggatc ttctgttgtt aacgcgcttt
    18661 ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc
    18721 gtggagctgt agtggacgac ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg
    18781 ttcactttac gccagaccct gaaattttta cagagacaac tgagtttgat ttttcaaaat
    18841 tggctacccg gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata tcgattgaag
    18901 acaaacgaga agaagaacca gtcatcaatg aataccatta tgacggcggg attaaaagtt
    18961 atgtcgaata cttgaatgca aacaaaaccg tgatttttcc agaacctgta tatttggaag
    19021 gtgaacagca agatatcgca gttgaagtct ctatgcaata tacagacggc taccattcca
    19081 atatcatgag ttttgccaac aatatccata cgtatgaagg cggaacacat gaatcaggat
    19141 tcaagactgc tttaactcgt gtgataaatg attatgcccg aaaacaaaag ctgatgaaag
    19201 aaaatgacga caacttgacc ggtgaagacg tgcgagaagg attgacagct gttatttcta
    19261 tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc aattccgaag
    19321 tacggacagt cactgatcgg ttatttagcg aacatttcat gaaattcttg ctggaaaacc
    19381 caagtgtggg ccgtcaaatt gttgaaaaag gattactagc ttccagagct cgtctagcag
    19441 cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt agagattagt aatctgccag
    19501 gaaaattagc ggactgctct agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag
    19561 gagattcggc cggcggatca gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc
    19621 ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg
    19681 aagagatccg gtctttattt actgctatgg gtacagggtt tggagaagat tttgatgtat
    19741 cgaaagctcg ctaccacaaa ttagttatca tgactgatgc cgatgtcgac ggcgcgcata
    19801 tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt gaagctggat
    19861 acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat attacgtatg
    19921 tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca cttcctgcct
    19981 caccaaaacc aagcgttcag cgttataaag gacttggaga aatggatgat catcaactat
    20041 gggaaacaac tatggaccca agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta
    20101 ttgcagcaga tcagatattt gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat
    20161 tcatcgagga aaatgcacac tatgtgaaga acttagatat ttaatcatga aggaggaaaa
    20221 agaatgagtg aagaaatcag agaaaacatc catgatgtca atctaaccag cgaaatgaaa
    20281 gactccttca tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga
    20341 gatggtctga aacccgttca tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca
    20401 ccggataaag cccataaaaa atctgccaga atcgtcggag atgtcatggg taaataccat
    20461 cctcatggag actatgcgat ctatgagtca atggtacgga tggcacagcc attcagctac
    20521 cgttatatgc tagtggatgg tcatggaaac tttggttctg ttgacggtga cggtgccgcc
    20581 gctatgcgtt ataccgaagc acggatgagc aaaatcgcta cagaaatgct gcgtgatatc
    20641 aataagaaca ctgttgattt ccaaagcaac tatgatgata cagaaaaaga accagttgta
    20701 ctacctgctc gcttcccgaa tcttttagtg aacggaacaa cggggatcgc cgtaggtatg
    20761 gccacgaata tcccacctca taacttgtct gaggtagtag cggctatcga tttgctgatg
    20821 gataatcctg atgtgacgac caatgaattg atggaagtat tacctggacc agattttcca
    20881 acaggtggtt tggttatggg aaaatcagga atccgaagag cgtatgaaac aggtaagggt
    20941 tctattaccg tacgtgcaaa agttgagatt actgagatgc caaatggtaa agaacggatc
    21001 atcgtgacag aattgcctta tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa
    21061 ctgcacagag acaagcgtat cgaagggatc acagatttgc gggacgaatc ttcccgtgaa
    21121 ggtatgcgga tcgtcattga catccgtcgt gacgccagcg catctgttat cttgaataac
    21181 ttatataaat tgacatcctt gcaaaattca tttggattca atatgttggc gatcgaaaaa
    21241 ggtgtgccga aagttttgag cttgaaacaa attcttgaaa actatatcga acatcagcgc
    21301 gaagtcatca caagacggac agtctttgaa aaagaaaaag cagaagcaag ggcacacatt
    21361 ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa tcattgcgat tatccgcggg
    21421 tctaaatcag atgacgaagc aaaagcgacg atgatcgaac ggttcgattt atccgatcgc
    21481 cagtctcaag ctattttaga tatgcgttta cgccgattga ccggtttgga acgggaaaaa
    21541 atcgaaaatg aataccaaga actcttaaaa ctgattgaag atttaactga cattctggca
    21601 agaccagaac gtgttacaga aatcatcaag aatgaattag cagacttgaa tcaacgattt
    21661 ggtgataaac gacgcacaga attactagtc ggtgaggtct taagtctgga agatgaagat
    21721 ttaatcgaag aagaagaaat cgttattaca ttgacaaaca atggttacat caaacgtttg
    21781 gcaaataatg aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc
    21841 cacaatgacg acttcgtcaa aaatctggtt tcttgttcta cccatgatac attgttattc
    21901 tttacgaata acggaaaagt gtatcgggca aaaggatacg aaattccaga atacgggcgc
    21961 acagccaaag gaataccaat tatcaattta ctaggtatcg attcgagtga aagcatccag
    22021 gcaatcattg cggtgacggg agaagcagaa gaaggccact atctcttctt tacgacgcgt
    22081 caaggtacag taaaacgtac ttctgtcact gcttttgcta acatccgaag taatggtctg
    22141 atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg tgctactgac agatggtcaa
    22201 tctaatatca ttattggaac acataacggc tactctgtca ctttcaaaga agaagcagtc
    22261 cgcgatatgg gacgtacagc ttccggggtt agaggaatac gcctgcgtga ggaagattat
    22321 gtcgttgggg cagccttaat gaaggaagat caagaagtat tagtgatcac agaaaaaggt
    22381 tacggaaaac ggacaaaagt atcggaatat ccagtgaaag gccgcggagg taaagggatc
    22441 aaaacggcga acatcactga aaaaaatggt ccactagctg gattgaccac tgtttctgga
    22501 gatgaagaca tcatgctgat tacagataaa ggtgtcatta tccgtttcaa cgtatcgact
    22561 gtttctcaaa ctggacgttc aacattaggc gtacgtttga tgaagatgga agctgatacg
    22621 aaagttgtca cgatggcagc agtcgaacca gaagctgcag aacaaacagt tgaagaacaa
    22681 ggatcagaag aatagccatc aagactaatt gtgtaagcta gcaagaatga ccgattaaaa
    22741 aatctggtta ttcctgctgg cttttttgat tagttgaatt tctcttaaaa tattcgaaac
    22801 tgttcattct gtattgcttt tgaccaaaat agagtgtata atcagagctt gtgagtagtc
    22861 taattaatta gataactctc cttgctctta ggaacctaag agccgaaagt ccacagggag
    22921 gtgaatatca atggaaaaca cgaaatatga aatcatgtac attattcgtc caaacattga
    22981 tgaagaagca aaaactgctt taatcgaacg tttcgatgca atcttgaaag ataacggagc
    23041 agaagttatc gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa tgaacggtta
    23101 ccgtgaaggt atctatcaca tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa
    23161 tgaatttgac cgtcttgcta aaatcaacga cgatatcatt cgtcacatga ttgttaaaga
    23221 agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg atgagaggag atgactattt
    23281 tgattaataa tgttgtatta gttggacgtc tgacgaaaga tccagatttg cgttacacag
    23341 caagtggtac ggcagtcgca acatttactt tggctgtgaa tcgtaatttt acaaaccaaa
    23401 gcggcaatcg cgaggctgat tttatcaact gtgtgatttg gcgcaaatca gcagaaactt
    23461 tagcaaacta tgctcgtaaa ggaacacttt taggtgttac tggacgtatc caaactcgtt
    23521 cttatgataa tcagcaagga caacgtgtat acgtgacaga agtagtagct gacaacttcc
    23581 aattgctgga aagtcgttct gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt
    23641 ctggtcaatc agctggcgga tttggcggaa acaataactc taatcaaaca tctcaatcat
    23701 ccaacggtat gcctgatttc gaccgtgata cttctgaccc attcggctca agttcaacga
    23761 tcgacatttc agacgatgat ttaccattct aaacgaattt gataggaggg aaatagaatg
    23821 gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag actacattgc tgctaaccat
    23881 attgaatata tcgattataa agatatcgaa ttactaaaaa gatttatttc agaacgcggt
    23941 aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa aattactgtt
    24001 gcaatcaaac gcgctcgtat catgggcttg ctacctttcg ttagcgaaga ctaataaatc
    24061 ctaggaattg aatgactgat ccttttgatc agctattcaa ttcctttttt tctgtaaaaa
    24121 taacctctat tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca aattagtcat
    24181 atatgggagg aaaattatgg aaaataaaaa caatcattta tccccgaaac ctctcttcct
    24241 tatcctgttt atattaatcg aagtattggt agtcttaatt ttaccatttc gttttgttag
    24301 cgcttcggtc attctattga tgaatttatg gggtgttcgc tattggttga aaatgaaacg
    24361 ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa
    24421 tctagcttat gtctcagaag tcatgcctgt aggagtgatt gcttatcagc catcaagcca
    24481 aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg gattaacaga
    24541 ggaagaattg ctagatactt tttttactgc aaaagaaaaa aagaaaaacc aagtctatct
    24601 agcagataaa acatttcagt ttgatatgga tacagtaaag ggtgttgttt attttaatga
    24661 tattacccaa aaccagttgt taaaactcca tcagacgaac agtcagccgg tgattggtat
    24721 tttatctatt gataactatg cagatgccat tgataaattg gatgacaaag agatttctta
    24781 tttgaactct ttcgtcacta cactagtttc tgactggatg aatgaatacc atgtcttttt
    24841 caaaagaatc aacgcagaac ggtttttctt cgtagcacag atgatcgatc tgaaaaagat
    24901 gatggataaa aaatttgatt tgctggatcg ctttcgagca gaagctgaaa aacagagctc
    24961 gcctatcacc cttagtatgg ggattgctta tggctcaaga accctcgatg aaatcggtgg
    25021 agaagctcag aataatttag atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa
    25081 agaggtaaaa gaaggagcaa aacctgttta ttatgggggg aaatctgcta agaatgtcaa
    25141 acgcacgcgt gttcgttctc gagcgatgag taccgctttg cgtcggattt ttgatgaaac
    25201 aggcgatgtg tttatcatgg ggcacaagtt tccggatatg gatgctttag gctctgcttt
    25261 tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct tacatcgtta tcaacgagaa
    25321 cgagatcatt ccagatgtag aacggtgtct tgaagaaatc cataagcatc ctgaattaga
    25381 aatgcaacta atctctccag aacgtgccat agagctgaaa aaagaagagg acctcctcgt
    25441 gatggtggat taccataaac cttctctatc gatctctcag ggcctatatg agcaatttga
    25501 taagattgtg atcattgatc accatcgtcg tggggaagag tttccaagta aacctctgct
    25561 gacttatatc gaatcttcag cttcttcagc ttcagagcta gtttctgagc tgatccaata
    25621 ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc acattattgc ttgcagggat
    25681 ggttgtagat acaaatagtt tcaatgtccg tacaactgca cgaacttttg acgttgccag
    25741 ctatttaagg tcttgtggag cagacgcatc cttagtacaa tatttattga gctcggattt
    25801 agcgacatac ttggagatca gcaatttgat tgctggaagc gactatgtga cttctgatat
    25861 tgttgtggcg gcagggacag acgaaaaaga gtataatagt gttatagcag caaaaacagc
    25921 agatacattg ctttcgatga tcaatatcaa tgcagctttc gtcattacaa agcggacaga
    25981 cggcttgatt gggatcagtg ccagaagtac cggcacgatc aacgtccaaa tcatcatgga
    26041 atccttaggt ggcggaggac acttcacaaa cgcagctact caactggaaa atacgacagt
    26101 tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa aatatgaatc aaatatatga
    26161 taaggagtaa tccaaatgaa agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa
    26221 ggggacatca aagaagttcc agcaggttat gcacaaaatt atctaatcaa aaataacttg
    26281 gcaaaagaag caacgaaaag cagtatcagt cagttagcag gacaaaagaa agcacaagaa
    26341 aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa
    26401 gatactgtag tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc
    26461 ccttcaaaac aaattgctga agggttaaac aaacaataca aaatcaaatt agataaacga
    26521 aaaatcgaat tagaaaatcc gatccgttca ctaggctaca caaacgtacc tgttaagctt
    26581 catcatgagg tgacggctac gatcaaagtt catgtcgtac aagaataaca aatcttgata
    26641 gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag
    26701 aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat ttcagattga tttcggtttc
    26761 ttttttggga acagcaaaat agcataaaga aaacgagtat aataaaagag aactattttt
    26821 ttattacact ttcggtacga atgatgactt ttctctcatt ttcttgtaaa atgaagcaat
    26881 tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat
    26941 attgaagctg aacaagcggt tttaggctct gtttttcttg acgcagaaac gatcattgat
    27001 gccatggagt acatagagcc aaaagacttt tatcgtcgtg gccatcaaat tatttttgag
    27061 acaatgatcg agttaaatga tcgtaatgaa gcgattgacg tggttactgt caaagaccgg
    27121 ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa gttatctctc agacttggca
    27181 ttagcagttc cgacagcagc aaatatcgtc tactatgcga aaatcgttga agaaaaatca
    27241 ttgctgcgta cattgatcca gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga
    27301 gaagaagttc aaacaatttt agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa
    27361 agaaatcgaa gcggcttttt atctattgct gaagtactga actccacgat tgaaaatatc
    27421 gatcgactag ctcaaaacaa tgaagaaatc acaggtctac caactggcta tcaaggtctg
    27481 gataaaatga cagcaggttt ccagcccgaa gaactaatca ttcttgctgc acgtccagcg
    27541 gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg taggaacaaa aacagatcgt
    27601 tctgttgcga tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt
    27661 gcagaaggtt cgattgaggc cagccattta cgtacaggtc agctttcgga agaagaatgg
    27721 tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa atatttttat cgatgataca
    27781 ccagggatca aaatctctga aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc
    27841 aatctaggct tgatattgat cgattatttg cagttgattg aaggtaacgg taaagagaac
    27901 cgtcaacaag aagtatcaga tatctctcgc caactgaaaa aactggcgaa agagctgaaa
    27961 gtacctgtca ttgctctttc acagttatct cgtggagtag aacagcgaca agacaaacga
    28021 ccagtgctaa gtgatattcg tgaatccgga tcaattgaac aagatgcgga catcgtagcc
    28081 ttcttgtatc gagatgatta ttatgaaaga gaaggcggag atgacgatga tggaaacgag
    28141 ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga accgaagcgg tgcacgagga
    28201 acagttgaac tactgtttat caaagaatac aataagtttt cgtcgctttc gccaagagaa
    28261 gaattttaaa gaagaggttg tgagccttga gcaatcccga gagctctcgt aagaagatgc
    28321 aaggagtaac cgcaggaacg actagagtac ttaatttaaa cagatctcca aagggattgt
    28381 gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg tgttcaatca gctgaccctt
    28441 ggtattagct cagcaaacgt caaaatatga ggaactattt tgacgtttgt ttctctaata
    28501 ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa acggtgttct cctgccaact
    28561 cctcggtcac aagtcacaat atcatcaaac atgagaagca gttttttgaa attgctcctt
    28621 gtgactcaga gttaaacggc aggctcacaa cctctttctt aaacggtgtt caaaagcttg
    28681 catctgcggt aatacgccca atcaaacgaa aaatatgaga agctattttt gtttggctgt
    28741 tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg ttccaaaagt
    28801 agcttcttcg aaaaataagt cgaaatttca caaaaatttg agaagcaatt ttcgtacatt
    28861 ccttcttatt tcttgaagct gaccacttct gttacaacct cttttttaat aaccattcta
    28921 aaaagataaa gttcgttttt taagcgtttt tataaaataa aaataaaaat cattcgattt
    28981 tatttgaaat atgtatctat tattgataca atggttcagg attaaataat atagaacgag
    29041 gtgttcacat gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa
    29101 aaatcacaga ctttctcagt gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca
    29161 atgcagggca cacaattaag tttaacggtg tgacttataa actgcatttg attccttctg
    29221 ggatttttta taaagacaag atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat
    29281 cattggttaa agaattagct tatttacatg aaaaaggtgt agaaacaagt aatttgcgta
    29341 tttctgaccg cgcgcacgta atcttgcctt atcatatcaa attggaccaa ttacaagaag
    29401 atgctaaggg cgaaaataag attggtacaa cgattaaagg aatcggacct gcctatatgg
    29461 ataaagcagc tcgtgtaggt atccgtattg cagacttatt agataaagaa atttttgaag
    29521 aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag
    29581 aaccacttga ttttgacgat atttttgaag aatattatga atacggacaa caaatcaaac
    29641 aatacgtcac agatacttcc gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt
    29701 tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca gggaacttat ccttttgtta
    29761 cttcttctaa cccagtagcc ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa
    29821 tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc
    29881 caactgaatt atttgatgaa acaggagaac aaatccgcga agtaggacga gaatacggta
    29941 cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg cgccattcta
    30001 aacgagtttc aggtattacc aacctatctt taaactcgat cgatgtatta agtggattgg
    30061 aaacagtaaa aatttgtacg gcttatgaat tagatggcga actaatctac cactatccag
    30121 caagcttgaa agagttgaac cgctgcaaac caatctatga agaattgcca ggctggtcag
    30181 aagacattac aggatgcaaa acacttgcag aactgccaga aaacgcacgt aactatgtac
    30241 gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa
    30301 cacaaacaaa catcttagaa agcgtctggg cgcaaatcta aggataaaaa cgacgactga
    30361 atcaggacgt tcttaccaga gcgagtcgaa gtgtttatct atacgataat aataacttga
    30421 acactgttta ttcagaataa gggactgtga caaaaaccgt gtcacagtcc ctttttccga
    30481 acaaacggtg ttcaaaagct tgcatc
//
LOCUS       DACBZN010000020        30068 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.020, whole genome shotgun sequence.
ACCESSION   DACBZN010000020 DACBZN010000000
VERSION     DACBZN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.020"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            59..517
                     /locus_tag="IVD64_000943"
     CDS             59..517
                     /locus_tag="IVD64_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP6148212.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            626..1309
                     /locus_tag="IVD64_000944"
     CDS             626..1309
                     /locus_tag="IVD64_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6148213.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            1456..2070
                     /locus_tag="IVD64_000945"
     CDS             1456..2070
                     /locus_tag="IVD64_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP6148214.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            2086..2292
                     /locus_tag="IVD64_000946"
     CDS             2086..2292
                     /locus_tag="IVD64_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148215.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            2833..3963
                     /locus_tag="IVD64_000947"
     CDS             2833..3963
                     /locus_tag="IVD64_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6148216.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            4055..4810
                     /locus_tag="IVD64_000948"
     CDS             4055..4810
                     /locus_tag="IVD64_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6148217.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            4868..6907
                     /locus_tag="IVD64_000949"
     CDS             4868..6907
                     /locus_tag="IVD64_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP6148218.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(7016..8167)
                     /locus_tag="IVD64_000950"
     CDS             complement(7016..8167)
                     /locus_tag="IVD64_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6148219.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(8321..8851)
                     /locus_tag="IVD64_000951"
     CDS             complement(8321..8851)
                     /locus_tag="IVD64_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148220.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(8950..9300)
                     /locus_tag="IVD64_000952"
     CDS             complement(8950..9300)
                     /locus_tag="IVD64_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP6148221.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(9457..10986)
                     /gene="ahpF"
                     /locus_tag="IVD64_000953"
     CDS             complement(9457..10986)
                     /gene="ahpF"
                     /locus_tag="IVD64_000953"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP6148222.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(10996..11559)
                     /gene="ahpC"
                     /locus_tag="IVD64_000954"
     CDS             complement(10996..11559)
                     /gene="ahpC"
                     /locus_tag="IVD64_000954"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP6148223.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(11703..12365)
                     /locus_tag="IVD64_000955"
     CDS             complement(11703..12365)
                     /locus_tag="IVD64_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6148224.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            12549..14195
                     /locus_tag="IVD64_000956"
     CDS             12549..14195
                     /locus_tag="IVD64_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6148225.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            14186..15268
                     /gene="uxuA"
                     /locus_tag="IVD64_000957"
     CDS             14186..15268
                     /gene="uxuA"
                     /locus_tag="IVD64_000957"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6148226.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(15376..16929)
                     /locus_tag="IVD64_000958"
                     /pseudo
     CDS             complement(15376..16929)
                     /locus_tag="IVD64_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            17077..18218
                     /locus_tag="IVD64_000959"
                     /pseudo
     CDS             17077..18218
                     /locus_tag="IVD64_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(18344..19423)
                     /locus_tag="IVD64_000960"
     CDS             complement(18344..19423)
                     /locus_tag="IVD64_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6148227.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(19526..19966)
                     /locus_tag="IVD64_000961"
     CDS             complement(19526..19966)
                     /locus_tag="IVD64_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6148228.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(20023..20433)
                     /locus_tag="IVD64_000962"
     CDS             complement(20023..20433)
                     /locus_tag="IVD64_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6148229.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(20613..21047)
                     /locus_tag="IVD64_000963"
     CDS             complement(20613..21047)
                     /locus_tag="IVD64_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6148230.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(21147..21995)
                     /locus_tag="IVD64_000964"
     CDS             complement(21147..21995)
                     /locus_tag="IVD64_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6148231.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            22384..24150
                     /locus_tag="IVD64_000965"
     CDS             22384..24150
                     /locus_tag="IVD64_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP6148232.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            24252..25511
                     /locus_tag="IVD64_000966"
     CDS             24252..25511
                     /locus_tag="IVD64_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6148233.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            25658..26959
                     /locus_tag="IVD64_000967"
     CDS             25658..26959
                     /locus_tag="IVD64_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148234.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            26962..27813
                     /locus_tag="IVD64_000968"
     CDS             26962..27813
                     /locus_tag="IVD64_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148235.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            27826..28641
                     /locus_tag="IVD64_000969"
     CDS             27826..28641
                     /locus_tag="IVD64_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP6148236.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            28792..29958
                     /locus_tag="IVD64_000970"
     CDS             28792..29958
                     /locus_tag="IVD64_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6148237.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT        10156 a         5365 c         5674 g         8873 t
ORIGIN      
        1 ttgtttatac tagtatctgg atggtttttt cattataaaa agcaaagggg tggatgttat
       61 gtatcagcaa gtagaagaat atgtcaaaaa gttccaagaa aaatatccta gttatcaatc
      121 agaaaaatta ggtttctttt catttgggga tacagaaaaa atggcagacg agttgagcga
      181 actagttgta aaaaagataa agactggtac atcaagtggt tacgatctgt ataaacaaga
      241 gaatgagcct ttaccaaaag ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc
      301 agtttgtatc atcaaaaata taaatgtgac catcttgcct tttagtgaag tcacgaaaga
      361 gcatgcttgg aaagaaggag aaggggatcg gacattagct tattggcgga aagtccatgc
      421 tgagtttttt aaacatgctt ataaagaagc gttgaacata gaatttacag aagagaagct
      481 ggtcgtatac gaagaatttg aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa
      541 tagctcatta ttgtctcctg aatagaaaag ggttacattt ttatatgaag ctgtattttt
      601 atataacatt actggaggag atattgtgga tacactggaa caaatcaaag gcggagtcgt
      661 cgtttcttgt caagctttgg aaaacgagcc gctacacagt tcttttatta tggggagaat
      721 ggctttggct gcaaaagaag gcggagcagt tggtattcga gcaaatacga cagaagatat
      781 caatgaaatc aagcgaatcg ttgatttacc ggtaataggg attatcaaaa gagattacga
      841 tgggtcagaa gtgtttatta ctgcgacaga aaaagaagtg gaagaacttt tggagacgca
      901 agcagaaatc attgcactgg atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc
      961 tgaacttgtc caaatgattc acgaacacgg tcggttagct atggcagata tttcgacttt
     1021 tgaggaagcg cttcaagcgc aagaagatgg ttttgatctt ttgtctacga ctttagcagg
     1081 ctatacagaa tattcacgaa agacgcagac acctgatttt gatttattga atcaaatcat
     1141 agaacaagtc gatatcccag taattatgga aggacatact gactctcctg aacaagtaga
     1201 gaaagcatat gaattaggtg ctttttcagt cgtagttgga tctattatca cccgtccgca
     1261 attgatcact aaaagatatg tcgaagccgc aagtaaagca aataactaaa aaacacgcac
     1321 ggcacagatt cttgacaaaa aagaaaaatt gatagatagt ttaaagcaga ttctaagaag
     1381 atgaaaaaag cattactttt cattttgtct tcaagatctg ctttttaacg aattttatag
     1441 aaagaaggcg aaaccatgtt tgaacggagt ccttcagaat tgacagaacg tgaaaattac
     1501 aagttgctga ttggttcagt catccctaga ccagtagcag tagtaacgac taaatcagca
     1561 aaagatatag tgaatatcgc acccttcagt tacttcaata tcgtcagttc gaatcgaccc
     1621 atcttatcct tggcgatcca gagaaaacaa gatgcagtaa aagacacagc aaagaatatt
     1681 cttgagacaa gagaagctgt tatccatatc cttgatgagg acaacgtaga agaagtaaat
     1741 aaaacagcag ccaatctgcc taatgaggaa agcgaactgt acctgactga cttgacttta
     1801 actgattcag taatcatttc agtaccagca ttgacagaag caaaagttcg ttttgaaaca
     1861 tccttatatg aacatgtcga gatacggaat aaaaataaag taacagctga tttatttttg
     1921 ctagaagtac agaaatatca tttaaatgaa acagtctatg atgataagac aggaaggatc
     1981 gatccaagaa aattgaatgc tgtaagccgt ttggcaggaa atgattatgc agcaatagga
     2041 gaaattttca cgattgcacg tcccgaataa aaaggagcac tagttatgtt aaaaataagc
     2101 tataaaccat cgacagattc aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt
     2161 ctgcaagggc agtacttaga agttccgcca ttgcaagacc attttgtcgt tactactgtt
     2221 actttagatg gtaaggaaat agaaatgcca gatcaaacga ttagcggatt gtttaattac
     2281 tttaacaaat aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac acataaaact
     2341 aaaaaattat caaagaatcc tggtgcaact gaaagaattt gcaacgggat tctttcaact
     2401 tatttgttat ttttttctaa aaaatattaa agattgttaa atttcactat ttcatttcaa
     2461 attccattgc ctaatcatta ggaaaagtct atcatgaact cgtaaataga atatagtaat
     2521 aaccttgtta ggtgaggctc ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc
     2581 gccaatgggt aaaacagaaa ctgttggatt aaggcttttt ccaatagcta agaatgtttt
     2641 ctttacgttg tatagtgcta aaactcggac gatgagatga tgctaggtca gaattcttgt
     2701 tcgaaaaggt ttctgacctt ttctttatat ttttgtgact gacttatttg atgagacaaa
     2761 ctcgctcctc acctgacaag tggattgctg tgtgaggagg agatgcggtg aaaaaagatc
     2821 gattggtgga ttttgggtcc ttatttgact ttgtccatga tcggtctttt agaagtatac
     2881 agtgccagtt cttatcgttt attgcaagcc gatgaaaata caaaaagtct attattgcgt
     2941 caactaattt tcatattttt gagttggagc gtgatcttct tagctcgttc agtcaaacta
     3001 cactatttac ttcaccctaa aatagcagga tacggtttag ccttatcgat tttcttttta
     3061 gtattagtaa gaatagggat attcggtgtc actgtcaacg gcgcacaacg ttggatctct
     3121 ctgtttggca ttcaattcca gccttctgaa ctggcaaatc tttttttgat tttttattta
     3181 agctggtttt ttcgtgacgg aaatagtagc ccaaaagatc taaaaaaacc attcctgatt
     3241 acagtaggta taacttttct gattttattt cagccaaaga ttgctggagc attgatgatc
     3301 ctttcgattg cgtgggtcat attttgggca gcggcggttc catttaaaaa agggatctat
     3361 ctaatcgtta ctttttctgc attgctgatt ggagcagcag gcggggtatt atatttagga
     3421 aataaaggtt ggcttccaca gatgtttaat catgcttatg aaaggatcgc gactttaaga
     3481 gattctttta tcgatagtca tggtgcgggc tatcaaatga cccattcgtt ttatgcacta
     3541 tataatggtg gcatatttgg gcggggatta ggaaatagta tcacaaaaaa agggtatctt
     3601 ccagagacag aaacagattt tattttttcg attattacag aagagcttgg gctaatcggt
     3661 gcattgtgtg tgcttttctt attgttctca ttatgcatga gaatcttttg tttaagcagt
     3721 cgctgtaaaa atcagcaagc tggattgttt cttttaggat tcggaacttt gctattcgtt
     3781 cagacgataa tgaacgtggg aagtatcgcg gggctgatgc caatgactgg tgtgccattg
     3841 ccatttgtta gctacggagg aacaagttat ctgatccttt ctttaggaat cggaatcacg
     3901 ttgaatattt cttcaaagat acaggccgaa gagttggcgc tctatcgacc tgaaaaacaa
     3961 taaagagaaa ctgagaaact ctcagtttga attatcataa agtacaatct tttatttttc
     4021 aagcgagagg ttcttttaga aagaggaatt cgcaatgaaa gtatctcaaa aaatcatatt
     4081 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
     4141 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
     4201 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
     4261 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
     4321 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
     4381 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
     4441 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
     4501 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
     4561 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
     4621 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
     4681 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
     4741 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
     4801 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
     4861 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
     4921 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
     4981 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
     5041 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     5101 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     5161 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     5221 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     5281 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     5341 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     5401 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     5461 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     5521 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     5581 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     5641 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     5701 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     5761 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     5821 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     5881 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     5941 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     6001 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     6061 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     6121 tgctcctgga tctacgttta aaatgattac agcagcaatc ggtctcgaca acggcactat
     6181 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     6241 atcttatcaa gcaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     6301 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     6361 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     6421 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     6481 atatggacag ggcgaacttc taattactcc tatccagcaa gcagcaatgt attctgtttt
     6541 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     6601 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     6661 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     6721 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     6781 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     6841 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     6901 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     6961 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     7021 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     7081 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     7141 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     7201 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     7261 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     7321 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     7381 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     7441 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     7501 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     7561 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     7621 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     7681 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     7741 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     7801 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     7861 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     7921 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     7981 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     8041 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     8101 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     8161 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     8221 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     8281 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     8341 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     8401 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     8461 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     8521 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     8581 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     8641 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     8701 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     8761 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     8821 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     8881 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     8941 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     9001 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     9061 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     9121 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     9181 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     9241 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     9301 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     9361 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     9421 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     9481 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     9541 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     9601 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     9661 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     9721 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     9781 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     9841 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     9901 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     9961 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
    10021 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
    10081 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
    10141 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
    10201 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
    10261 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
    10321 tgcactacta gctgctggtc caccgccaat cactaataca tcataaggtt ctttttcaaa
    10381 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
    10441 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
    10501 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
    10561 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
    10621 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
    10681 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
    10741 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
    10801 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
    10861 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
    10921 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
    10981 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
    11041 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
    11101 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
    11161 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
    11221 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
    11281 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
    11341 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
    11401 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
    11461 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
    11521 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
    11581 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
    11641 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
    11701 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
    11761 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
    11821 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
    11881 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
    11941 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
    12001 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
    12061 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
    12121 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
    12181 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
    12241 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
    12301 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
    12361 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
    12421 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
    12481 atagcgattt ttattgcata aaattcactt gaatacaaaa actaatatgt tagtttagga
    12541 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
    12601 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
    12661 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
    12721 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
    12781 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
    12841 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
    12901 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
    12961 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
    13021 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
    13081 ttcttgctgt gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
    13141 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
    13201 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
    13261 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
    13321 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
    13381 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
    13441 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
    13501 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
    13561 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
    13621 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
    13681 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
    13741 caaatattcc taatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
    13801 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
    13861 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
    13921 gaaaaccatt catcccgagt ccagacccac tattgacaga ccttcagcat aagctttccg
    13981 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
    14041 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    14101 aaatgttcat caagatggta gcaggaccaa atgccgtacg aaaaacatta caggattact
    14161 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    14221 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    14281 attgatggat ataccagtag gagaggtatg gcctagggag aggatcaaag aactaaaaga
    14341 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    14401 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    14461 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    14521 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    14581 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    14641 atttggattg ccgggatggg aacctgagcg cttagctcct atcaaatcct tgcttgaagc
    14701 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    14761 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    14821 ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgtcg
    14881 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    14941 agaaaatgat gtatatggga ttttagcaga attttgccaa agagatcgaa tcccatttgc
    15001 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cacataaatc
    15061 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    15121 cggatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    15181 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    15241 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    15301 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    15361 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    15421 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    15481 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    15541 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    15601 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    15661 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    15721 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    15781 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaatac
    15841 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    15901 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    15961 aggtataaac aaatcaaata cagtacaaag ggactccacc atttctcttg aataaagaac
    16021 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    16081 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    16141 tggcagatta ggtcgcaaaa tatctacaga gagatcaata aaaaagtcaa tatattcata
    16201 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    16261 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    16321 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    16381 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    16441 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtactatgc
    16501 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    16561 ctgactgaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    16621 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    16681 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    16741 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    16801 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
    16861 aaaaatatct aattttgtat tgcttttttt cttcaagata aacgttttaa tcgcttcttg
    16921 agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt
    16981 tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat
    17041 ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata
    17101 tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag
    17161 acagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag
    17221 gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc
    17281 atcaaagcaa aaattgacta ttcgggagac ttagcaccta tactacaatc agctgttaca
    17341 tttgagaatg ttgatcccac tttgtcgatt ggctatgacg agaacacttt gactatccaa
    17401 cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg
    17461 ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatttaac gccgtatagt
    17521 cagcggcaga caattgagca agattcagcc atcgactcag gaggagatct tgttgaagaa
    17581 ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga aaatccagac
    17641 ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac tcaaccagag
    17701 ccagacaata aatcaaaacc tgaaataacc cctggagaaa aaccagatgt aactccagaa
    17761 ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa
    17821 actgaaaatc cagaaaaacc agaggtagat ccagaagaaa aaccagatgt gactccagaa
    17881 ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa
    17941 gaggatggaa aaaaagagag taaaaaatca aatctagcaa ttctaaaaat aaatgaagag
    18001 cagctgaata aaaaatcacg aatatttgat agcgcccaat cagctgaaac cttaaaaagt
    18061 agcaaggata caacctttgc tgtcctgaaa cgaaaaataa acagctacca aaatccggag
    18121 agtcacaaaa caaagttata ctgtggagcg gaatcattct attaagtata gcaacaatgc
    18181 tttctgcaaa aagatttaaa caaaacagat ctctctaaca tttgaataat gtctgtgaac
    18241 gcatttttca aataaaaaga aatatccaga aaatgacccc aaaataccag attgaaatct
    18301 gatatttggg gtcgttcata ctttatcttt cttcatattt attttaaggg atatctgttt
    18361 cttttggaag atggttcggg atctttttca ctttcaatac atctaaaaag aaggtaaaga
    18421 tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca agtaagacca
    18481 caaatgtata aaaaatcggc agttctgttt tgctggacat caacttcgga ttcagcacat
    18541 aagactcaat tagatggaca atcaaaatcg taatcaggat ataaacaaca tctcggatgc
    18601 ctccttggga ataagctatg aaagatagag gaatacaaga aatgataaca cctgctactg
    18661 ggatcagact taaaatgaaa atcatgatcg ctaaactcag cagctgatga aagccaaata
    18721 cagccaaggc gattgtagtg agcaacgtat ttaaaagagc gacaacaaat tgagcttcta
    18781 ataccaaacc aaatgtattg acaaacttat cggcaaaata gtaaatatct tgaaagaacc
    18841 aagaaaattc actttttaaa aacaatcttg agaatacaat tgtttttttc ttttcaatca
    18901 taaaaaagaa acttaagatg aatgacatag caaacgccac tgccattttt cctatatcct
    18961 gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct
    19021 caatataagt atggatatat tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa
    19081 aattaataac ggactggatc atgtcaaatg tttgatgaat aagcatcggg atatactttg
    19141 tgaatgccag atagataagg taaacgatca gtgtataaat catgatagct aaaacacctg
    19201 tagggattcg gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa taagtaaaga
    19261 taaatgtgag caaaatggtt gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa
    19321 ataataaaac agtgagacgg cgtatttttt cattcgccaa aaaacgctga taaacggtca
    19381 aatcgacaac ctccaaataa caaaaaataa acacgttatt cattatatga tggaatatga
    19441 aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag ttggttttcc
    19501 tcataaaatc gttcatctag cagtttcagc cctttttctg gtcttgatga aaattatcta
    19561 gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt ggaagataag
    19621 acttgatctt ttgtggaata ccttctgctt gtttttgcaa cgcagcacct tgagcagtca
    19681 gcgtaacatt tacgattctt tcatcacccg tttcacgctg acgattaata taattttttt
    19741 ctgctaactt ttttagtaag ggagtcagcg taccagaatc cagacacaaa tcatttccta
    19801 gctctttcac tgaagtcttt ttcttttccc ataaaactaa catcacaaga tactgggtat
    19861 aggtaagatt cagcggcttg aggaggggag tgtagtgtct aatgatttct ttagaggcgg
    19921 catataaagg aaaacatact tgattaacta aatatagatc ttccattttt ccacctccgc
    19981 tgttttacta tagtgtatca tagcggattt acacaaaaaa gtctattcac taactgcttt
    20041 cagagtaact tctatatttc cagctgtagc ttttgaatac ggacacactt tatgcgctgc
    20101 ctctaataat tcttgcgttt cttccagaga aagaccttcg atagatcctt caatctctac
    20161 acctaagaca aagctcgttt cactttctga ataaagggaa acttgtgcac tgacagtaga
    20221 agccccagag atattttttt gctgtttgat caattctaat gcactgttaa aacatgcact
    20281 gaaaccagct gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt taccaggagc
    20341 tgcaatattc aattcgaatg tacgatcggg attatgcact tctccagagc gtccgcctgt
    20401 gttgatcatt ttcgtagaat acattttttt cataaaatta ccacctttaa ttatatttta
    20461 tacaattgaa ttttacacaa tataattgtg gaaagcaaag aaatggattg gcataagata
    20521 aataagagaa agcacatgaa acagacgcgc tttctcgaat agcaaacata tacaaaaaat
    20581 atcagagaca aaaaataatc tcatcataaa cattacatat atttctcaaa aagttgtttt
    20641 ctttcttcaa aaattacttc acgatggacc tgatgaagca tccataaata accaaaagga
    20701 tctctgaaca ttgagttagt cacaccaaag tccatcattt ctgtcatttc ttgaatctct
    20761 acacagccat ttcgtaatgc tttattatgt gtgtcctcga tatcagagac gatgatattg
    20821 aaccagatcg tatttggatg ttcagcagta ggagcagtca aaccaagaga cgtgttttca
    20881 tccagcatat ggaatctcgt accatacaag gtaaaaatga cttcattatt gcctctaggg
    20941 taatctgttg cctcaatcag ttgtatttca tcaaagattg tttgatacag ttctagtgct
    21001 ttcaaactat catttaatac aaaatcgatt tccgttcctt tatgcataaa gatctcctct
    21061 ccttttcagt cgaacagtta ctagattctt ttattatacc agctaaagaa aacgcattca
    21121 aaaaaatagc ggatttactt accaactcat tcgattggat gataagtaaa tccgcgtccc
    21181 attacttctg ctacgctgct gacaatgaca aaggcacttg gatcgatttc ttctatcaat
    21241 ggtttcattc gggaaaattc atgatcagtc acaacgatca ataaaacctc ttttgacttt
    21301 cgattatacc cgccttttgc ctctagcagg gtaaggccac gtccaatctc agcacttaag
    21361 cgaaggcgga tgtcttccaa ataatgttca ctcatgacca taacagattt ctttcgattg
    21421 atacctgttt cgatataagt catgacaatt gaagtgattg caatagataa aatagcataa
    21481 agaaactctt ctattccaaa aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa
    21541 aataagccaa tcgaagtatt caagtggaaa tattttttaa aaatgagggg agggatcgtc
    21601 gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca
    21661 cttccaaaaa tgacagacaa caaccgatct gaagtaatca ttgtttctgg aactattgca
    21721 atgattaaag gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt tttacctaaa
    21781 aatagaagag ctagtaccaa cataacgata ttcaacacta aaataactat agctcggtca
    21841 aagcctaaag cagcttccag taatattcct agaccgctga cacctccagc agcaatatga
    21901 tgaggcccta aaaacatatt gatagcgact cctaatatga agagggcaaa aataatctta
    21961 atgtatgtcc atataaattg tctcgtttct ttcatttatt cacttcctta attcattaat
    22021 aatactctta tcttattata acttactttt caccttaagt taactttaat taataaaaaa
    22081 attaggctag tctaaattat tatgtttcat tcttctagaa tatacttcta cgccaaaaac
    22141 tgttaaaaaa cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga
    22201 cactaaatag tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc
    22261 aagtttaaaa aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg
    22321 ttgtcttttg cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa
    22381 tcaatggata cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg
    22441 aatacaagat ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg
    22501 ctatgtggag atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg
    22561 aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca
    22621 agtcgcttac agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt
    22681 gatcaaggaa tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga
    22741 ataccttatt ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta
    22801 tggtatcaaa tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac
    22861 actttacctt ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa
    22921 ggtgtgatgg atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt
    22981 cccatattta aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat
    23041 cccgcttttg gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg
    23101 atacgtatca tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa
    23161 gacgttctca aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc
    23221 ccagtagatt cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat
    23281 acttttgctt ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa
    23341 tatttgctgg atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt
    23401 gatgttgcta atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa
    23461 atcaaaccag acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa
    23521 ggggatgaat tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt
    23581 gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt
    23641 tacagagatc aggtgaacga aggaacattc aacttattag attctcatga tactgccagg
    23701 atcttgaccc tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt
    23761 cttcaaaaag ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat
    23821 gacccggatt gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc
    23881 ggttttataa aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt
    23941 tctactcagt ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta
    24001 gagggacaga tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag
    24061 gaaaatgagg tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata
    24121 cgacaatacg gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt
    24181 ttgcaaatga agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg
    24241 aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag
    24301 gaatcttagg cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg
    24361 atgaaggaaa aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa
    24421 taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt
    24481 tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga
    24541 tggcagcata tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac
    24601 tgggaaatga agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa
    24661 tctacggaga acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg
    24721 atacagcacc atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt
    24781 ttgagtcaga agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt
    24841 cctatggttt aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt
    24901 cagatatcgg tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt
    24961 tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc
    25021 agcaattcat gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat
    25081 acaaagaaaa caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc
    25141 cttatcaacc gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag
    25201 acctctctca aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg
    25261 aaatggtaaa tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg
    25321 atgaattaac aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc
    25381 cagaaatggg cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt
    25441 caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac
    25501 aaaaatactg attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag
    25561 ataaaagtgg caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag
    25621 cagtttgcgg tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa
    25681 tatcaacgaa aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa
    25741 tggccaagct tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat
    25801 gcttctgggc ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga
    25861 agatcattca ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt
    25921 cttgattttt tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga
    25981 gggcaaaaaa atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt
    26041 tccttatcta ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt
    26101 cctagttaca ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag
    26161 tttaattgat tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg
    26221 taatactttt tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt
    26281 gcaaatcacc cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa
    26341 acgtattttc ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat
    26401 gagtttttcg aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt
    26461 cataaaccag cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg
    26521 gacgaaagta gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat
    26581 ggttacagga atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg
    26641 agcaaatgca tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc
    26701 gccaacattt attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt
    26761 taacaatggc ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc
    26821 ctggatctat aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac
    26881 gttgattatc tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc
    26941 gttcaatatg ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga
    27001 ctttctcact tttttactta cttttttctt ttaatactgt cagtcattat catctatccg
    27061 cttttgatca cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat
    27121 ctttcaagtg actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg
    27181 acttggtata aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta
    27241 gtaactttgg cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg
    27301 atgttctttt tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc
    27361 atggctttct tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc
    27421 ggtggaattc caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat
    27481 ttagatgaat ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta
    27541 ttaccgcttg tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt
    27601 ggggatttca tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc
    27661 ggcttacaaa catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg
    27721 gcaattttga ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt
    27781 accggcctga catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg
    27841 ttaaactatt tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta
    27901 tcatggccta aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg
    27961 actctttttt atgccaatca gatccaagcg attccattag aacaatttct atccgttcat
    28021 tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg
    28081 ttacaagcat cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta
    28141 tctgagaaac aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg
    28201 atccaacaaa aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc
    28261 caaccagatt ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat
    28321 gccagtaatc gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt
    28381 gtcatgacga gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc
    28441 caactttcat ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga
    28501 ataggttcca tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta
    28561 agtgtccaaa caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc
    28621 aaagataagc gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa
    28681 atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct
    28741 tttctttttt agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag
    28801 aaaactttat acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat
    28861 tgggacggta caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat
    28921 atccatgaag aaatcccaat gaaagagatt acaagtaacg catctttggc actaggagaa
    28981 gcctatatgg atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct
    29041 tatcagcaag caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa
    29101 gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac
    29161 gacttttata aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca
    29221 cctgaagata cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaagctc
    29281 tttatcaaag aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc
    29341 actgctacca aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac
    29401 gatttgatta ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta
    29461 atggattacc gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc
    29521 gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa
    29581 ccaaaaggaa cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct
    29641 tggatcaata aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat
    29701 aatattacaa aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa
    29761 ttgacattag aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa
    29821 atgggtgatt ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt
    29881 gaatcaagta atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc
    29941 ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct
    30001 aagttttatg atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag
    30061 cttagttt
//
LOCUS       DACBZN010000021        29153 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.021, whole genome shotgun sequence.
ACCESSION   DACBZN010000021 DACBZN010000000
VERSION     DACBZN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.021"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..1030)
                     /locus_tag="IVD64_000971"
     CDS             complement(<1..1030)
                     /locus_tag="IVD64_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6148238.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IVD64_000972"
     CDS             complement(1112..1801)
                     /locus_tag="IVD64_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6148239.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVD64_000973"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVD64_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP6148240.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IVD64_000974"
     CDS             complement(2328..2831)
                     /locus_tag="IVD64_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP6148241.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVD64_000975"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVD64_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP6148242.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVD64_000976"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVD64_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP6148243.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVD64_000977"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVD64_000977"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP6148244.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVD64_000978"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVD64_000978"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP6148245.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IVD64_000979"
     CDS             complement(6443..7462)
                     /locus_tag="IVD64_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6148246.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSTSGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8521)
                     /locus_tag="IVD64_000980"
                     /pseudo
     CDS             complement(7635..8521)
                     /locus_tag="IVD64_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IVD64_000981"
     CDS             complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IVD64_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP6148247.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IVD64_000982"
     CDS             complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IVD64_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP6148248.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IVD64_000983"
     CDS             complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IVD64_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6148249.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9752..10396
                     /locus_tag="IVD64_000984"
     CDS             9752..10396
                     /locus_tag="IVD64_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6148250.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10452..10955)
                     /locus_tag="IVD64_000985"
     CDS             complement(10452..10955)
                     /locus_tag="IVD64_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6148251.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10968..11014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11552..12517
                     /locus_tag="IVD64_000986"
     CDS             11552..12517
                     /locus_tag="IVD64_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6148252.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12616..13788)
                     /locus_tag="IVD64_000987"
     CDS             complement(12616..13788)
                     /locus_tag="IVD64_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148253.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13775..14920)
                     /locus_tag="IVD64_000988"
     CDS             complement(13775..14920)
                     /locus_tag="IVD64_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148254.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14934..15866)
                     /locus_tag="IVD64_000989"
     CDS             complement(14934..15866)
                     /locus_tag="IVD64_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148255.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15983..17062
                     /locus_tag="IVD64_000990"
     CDS             15983..17062
                     /locus_tag="IVD64_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6148256.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17059..17688
                     /locus_tag="IVD64_000991"
     CDS             17059..17688
                     /locus_tag="IVD64_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6148257.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17798..18025)
                     /locus_tag="IVD64_000992"
     CDS             complement(17798..18025)
                     /locus_tag="IVD64_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6148258.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18128..20716)
                     /locus_tag="IVD64_000993"
     CDS             complement(18128..20716)
                     /locus_tag="IVD64_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP6148259.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20736..21395)
                     /locus_tag="IVD64_000994"
     CDS             complement(20736..21395)
                     /locus_tag="IVD64_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6148260.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21444..21947)
                     /gene="trmL"
                     /locus_tag="IVD64_000995"
     CDS             complement(21444..21947)
                     /gene="trmL"
                     /locus_tag="IVD64_000995"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP6148261.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22168..23034)
                     /locus_tag="IVD64_000996"
     CDS             complement(22168..23034)
                     /locus_tag="IVD64_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6148262.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23143..23784
                     /locus_tag="IVD64_000997"
     CDS             23143..23784
                     /locus_tag="IVD64_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP6148263.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23846..25207)
                     /gene="mgtE"
                     /locus_tag="IVD64_000998"
     CDS             complement(23846..25207)
                     /gene="mgtE"
                     /locus_tag="IVD64_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP6148264.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25225..26124)
                     /locus_tag="IVD64_000999"
     CDS             complement(25225..26124)
                     /locus_tag="IVD64_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6148265.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26126..26923)
                     /locus_tag="IVD64_001000"
     CDS             complement(26126..26923)
                     /locus_tag="IVD64_001000"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP6148266.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26901..27605)
                     /locus_tag="IVD64_001001"
     CDS             complement(26901..27605)
                     /locus_tag="IVD64_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP6148267.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27736..28311
                     /locus_tag="IVD64_001002"
     CDS             27736..28311
                     /locus_tag="IVD64_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP6148268.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28429..29106
                     /locus_tag="IVD64_001003"
     CDS             28429..29106
                     /locus_tag="IVD64_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6148269.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8582 a         6171 c         5242 g         9158 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgtactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtccgctg tagccgtgga
     8341 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
     8401 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
     8461 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
     8521 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
     8581 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
     8641 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
     8701 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
     8761 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
     8821 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
     8881 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
     8941 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
     9001 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
     9061 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
     9121 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
     9181 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
     9241 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
     9301 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
     9361 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
     9421 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
     9481 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
     9541 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
     9601 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
     9661 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
     9721 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
     9781 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
     9841 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
     9901 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
     9961 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    10021 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    10081 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    10141 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    10201 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    10261 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    10321 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    10381 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    10441 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    10501 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    10561 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    10621 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    10681 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    10741 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    10801 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    10861 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    10921 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    10981 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    11041 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    11101 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    11161 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    11221 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    11281 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    11341 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    11401 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    11461 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat
    11521 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
    11581 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
    11641 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
    11701 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
    11761 cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga
    11821 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaaa
    11881 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
    11941 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
    12001 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
    12061 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
    12121 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
    12181 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
    12241 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
    12301 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
    12361 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
    12421 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
    12481 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
    12541 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
    12601 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
    12661 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
    12721 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
    12781 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
    12841 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
    12901 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
    12961 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
    13021 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
    13081 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
    13141 gtttcgttta ctgatctctt tgcgattaaa cgctagattg atgacgctga aaccgctaaa
    13201 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
    13261 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
    13321 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
    13381 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
    13441 tatcttgatc gtcgccatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
    13501 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
    13561 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
    13621 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
    13681 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
    13741 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
    13801 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
    13861 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
    13921 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
    13981 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
    14041 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
    14101 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
    14161 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
    14221 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
    14281 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
    14341 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
    14401 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
    14461 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
    14521 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
    14581 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
    14641 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
    14701 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
    14761 ttccagtatt aatcgtttca agattgccac cttgatcaat ctcgccaaag gccgccataa
    14821 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
    14881 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
    14941 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
    15001 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
    15061 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
    15121 attgttcgct gaatcgctga caacacattt tctgggagta atgggatgtc gatcgtcaaa
    15181 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
    15241 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
    15301 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
    15361 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
    15421 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
    15481 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
    15541 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
    15601 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
    15661 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
    15721 agcatacagt tgatggcagt cgatttccca ctgccgtttg gacctaacag acctaagact
    15781 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
    15841 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
    15901 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
    15961 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
    16021 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
    16081 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
    16141 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
    16201 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
    16261 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
    16321 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
    16381 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
    16441 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
    16501 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
    16561 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
    16621 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
    16681 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
    16741 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
    16801 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
    16861 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
    16921 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
    16981 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
    17041 accaagggag ggacaccaat gataacagta gtcattattg atgatgatcc atttgtgacg
    17101 aaatcgttac aaaccatttt agaaagcacg aaagagatcc gtgtgttagg cattggtcat
    17161 tgtgccaagg atgcgctagt cttatacgaa acccatcgtc ccgatgtttt gctgacggac
    17221 atccgcatgc ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc
    17281 aaagccatcc ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt
    17341 tccatcggtg tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc
    17401 aaatccgctt ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa
    17461 atgatgaaga ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag
    17521 cttgcaatca tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg
    17581 tacttgagtg acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta
    17641 cgggatcgca cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac
    17701 acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc
    17761 aattgtcccg caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag
    17821 gattcgttgg attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt
    17881 ctagttttga ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt
    17941 ctttttccat ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta
    18001 atttatcttc ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat
    18061 atcacactga aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc
    18121 tttaaaatca agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa
    18181 tcatcggatc tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa
    18241 cagcaggttc tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt
    18301 ccactgtttc cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta
    18361 gcttttcttt tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc
    18421 cgaccaaaga atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat
    18481 tatccatgat ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag
    18541 tgacataggc ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg
    18601 tgtagagcaa attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca
    18661 tcttgaattc actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat
    18721 tccattcgtt tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca
    18781 ctttatcttc agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga
    18841 atacattcaa ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg
    18901 tgtcgttcca atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca
    18961 ttttatttaa cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat
    19021 cttgtgcacg gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa
    19081 tttgataagc atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg
    19141 gcagacgtcc ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc
    19201 gataaatctg attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta
    19261 cttgaccagg accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat
    19321 tcgtcggaat cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat
    19381 caacaatcaa aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc
    19441 cattcagccc cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc
    19501 gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt
    19561 ttgaaggatc caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca
    19621 cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt
    19681 cagcgatagc ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc
    19741 gaatattctt ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc
    19801 gctggatcga attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag
    19861 tctcttcttg ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat
    19921 caggctggat ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg
    19981 tctccgccgg tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga
    20041 tagctgctcg aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat
    20101 ctgcacgttt gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga
    20161 tgatatccaa agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga
    20221 acccgtaacg attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct
    20281 cagcaagcat ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat
    20341 cagcaatgat tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt
    20401 tcttcccgat tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg
    20461 ttggcttttc ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca
    20521 ctaattctcc ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga
    20581 ctacaatctc tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt
    20641 tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca
    20701 tttcttctgg ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt
    20761 tcagttccta aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa
    20821 ggctcttcag tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac
    20881 tcacttagct cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct
    20941 acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata
    21001 cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa
    21061 tgattacgca aattcggcag attatccgga tatttctggt acatttcatc gatcgactga
    21121 ccttctaaag tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga
    21181 cgagcaagct ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa
    21241 taaattgctt taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa
    21301 ctttgtggca aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat
    21361 tgtgtcttcc catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt
    21421 tattttactc tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat
    21481 tctaaattag ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc
    21541 gataaattca atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg
    21601 ttttcacgca taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc
    21661 tcttctccat cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa
    21721 tgtagtttac gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata
    21781 tttacatcat tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa
    21841 cctagtggct cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt
    21901 ccagtgtttg cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct
    21961 ctcatattta tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta
    22021 tcaactcggt gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt
    22081 atcagaacag gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc
    22141 tcaacttttt tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt
    22201 tccttttaat aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc
    22261 tttgattcgc tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt
    22321 ttcctctggt atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc
    22381 agcaaacttg gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata
    22441 aaaagcctga cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc
    22501 atcttctgag agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac
    22561 cgcgttgaat gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc
    22621 ttcggcagat tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc
    22681 attttgaatc tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac
    22741 atctaatagc gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata
    22801 catgccagat tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt
    22861 aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt
    22921 tttgcatatc aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa
    22981 cagctgatga ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc
    23041 ccctcatttc tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga
    23101 tacaatgcct agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc
    23161 gctgaaaact acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg
    23221 tgtgacaact tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta
    23281 agttatgctg gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac
    23341 ttcgtctata acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa
    23401 ttagggacgg atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag
    23461 gccttagaac tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt
    23521 gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt
    23581 gaccgtatct tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg
    23641 cttaaagaat tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc
    23701 actgcagaga atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc
    23761 aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat
    23821 gcttattgca aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat
    23881 tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta
    23941 ctgctggatc aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg
    24001 ccactgtgat tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt
    24061 tccaaaaacc tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg
    24121 tcaagacttc agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa
    24181 gtcgacgaac agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca
    24241 gcgaaatgaa gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta
    24301 aagaagcggt cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg
    24361 ctgcctttac tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat
    24421 cgctagcagc ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca
    24481 aatgatcatc ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg
    24541 ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag
    24601 aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct
    24661 gaccttcttg attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct
    24721 tcagcacgta catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg
    24781 tcatgatcgc gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat
    24841 cgtctgccga catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca
    24901 agtcaacagc attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca
    24961 tttcatctag gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg
    25021 ctaattcttt aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt
    25081 ggtagaattg cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa
    25141 tattattttc ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt
    25201 catccaaacc aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc
    25261 tgcggctaaa ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag
    25321 atcataacaa tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc
    25381 atcaccgagc agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc
    25441 tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc
    25501 agtctgtgcc gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg
    25561 ggcgatcggc aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa
    25621 ggcctgatat tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc
    25681 aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac
    25741 atggattact tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat
    25801 tgtcccattt ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac
    25861 cagaacgtgt tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc
    25921 atgttctcct tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa
    25981 gacattttct acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa
    26041 cagacccttt gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt
    26101 ctcataatcc catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat
    26161 gccaaaaatg catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat
    26221 aaattgagcg gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt
    26281 ctgtattttc caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca
    26341 aagtacgaaa cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac
    26401 taggatggat cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag
    26461 aaatcgtcaa tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg
    26521 ctaccatcgt acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt
    26581 ccggtgtttc atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt
    26641 cccggttcgt tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt
    26701 aaaaccctaa atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt
    26761 agtgatggaa cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct
    26821 catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt
    26881 ctatagtctt tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt
    26941 tcgctttctt tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg
    27001 gagaataacc gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac
    27061 tgataggcag cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct
    27121 tgatatttat aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga
    27181 atctggattt ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc
    27241 accagatgat aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg
    27301 agaactttca tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca
    27361 aactgacaca tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt
    27421 ggaatatgcc gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg
    27481 acaaattcaa tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt
    27541 ttcactttca attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt
    27601 tccatagctg atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag
    27661 ttttttcgta ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa
    27721 aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag
    27781 caagagtacg agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag
    27841 actaatattt atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg
    27901 atccgtttcg ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt
    27961 ctcgaaacaa cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt
    28021 ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca
    28081 ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat tccctattga agaaggactt
    28141 ttagctattg atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta
    28201 ggggatgcat ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct
    28261 tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc
    28321 attttcacaa ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga
    28381 aagaatctag tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc
    28441 tatttatttg ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa
    28501 tttatcgaag aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct
    28561 ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat
    28621 cttgaggaac gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg
    28681 gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc
    28741 ggctgtaatg acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc
    28801 ggcgatttga atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt
    28861 tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac
    28921 aattatgcct gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt
    28981 tggagaatca cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa
    29041 catttacata caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct
    29101 aattaaaaaa ggctctttgt caaatgatgt tggtaaagag aaatcttaga ata
//
LOCUS       DACBZN010000022        29141 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.022, whole genome shotgun sequence.
ACCESSION   DACBZN010000022 DACBZN010000000
VERSION     DACBZN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.022"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..680)
                     /locus_tag="IVD64_001004"
     CDS             complement(<1..680)
                     /locus_tag="IVD64_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6148270.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELR"
     gene            complement(746..2035)
                     /locus_tag="IVD64_001005"
     CDS             complement(746..2035)
                     /locus_tag="IVD64_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6148271.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(2122..2826)
                     /locus_tag="IVD64_001006"
     CDS             complement(2122..2826)
                     /locus_tag="IVD64_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP6148272.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            3026..3292
                     /locus_tag="IVD64_001007"
     CDS             3026..3292
                     /locus_tag="IVD64_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6148273.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(3460..3690)
                     /locus_tag="IVD64_001008"
     CDS             complement(3460..3690)
                     /locus_tag="IVD64_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6148274.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            3762..3917
                     /locus_tag="IVD64_001009"
     CDS             3762..3917
                     /locus_tag="IVD64_001009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148275.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(4226..4450)
                     /locus_tag="IVD64_001010"
     CDS             complement(4226..4450)
                     /locus_tag="IVD64_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6148276.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            4717..5052
                     /locus_tag="IVD64_001011"
     CDS             4717..5052
                     /locus_tag="IVD64_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148277.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5285..6226
                     /locus_tag="IVD64_001012"
     CDS             5285..6226
                     /locus_tag="IVD64_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP6148278.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            6228..7118
                     /locus_tag="IVD64_001013"
     CDS             6228..7118
                     /locus_tag="IVD64_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP6148279.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            7161..7961
                     /locus_tag="IVD64_001014"
     CDS             7161..7961
                     /locus_tag="IVD64_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP6148280.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7976..8827
                     /locus_tag="IVD64_001015"
     CDS             7976..8827
                     /locus_tag="IVD64_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6148281.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8824..9141
                     /locus_tag="IVD64_001016"
     CDS             8824..9141
                     /locus_tag="IVD64_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148282.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            9134..9316
                     /locus_tag="IVD64_001017"
     CDS             9134..9316
                     /locus_tag="IVD64_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148283.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            9318..9788
                     /locus_tag="IVD64_001018"
     CDS             9318..9788
                     /locus_tag="IVD64_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148284.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            9785..10327
                     /locus_tag="IVD64_001019"
     CDS             9785..10327
                     /locus_tag="IVD64_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP6148285.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            10324..10707
                     /locus_tag="IVD64_001020"
     CDS             10324..10707
                     /locus_tag="IVD64_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148286.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            10704..10934
                     /locus_tag="IVD64_001021"
     CDS             10704..10934
                     /locus_tag="IVD64_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148287.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            10941..11144
                     /locus_tag="IVD64_001022"
     CDS             10941..11144
                     /locus_tag="IVD64_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP6148288.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            11141..11437
                     /locus_tag="IVD64_001023"
     CDS             11141..11437
                     /locus_tag="IVD64_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148289.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            11514..11927
                     /locus_tag="IVD64_001024"
     CDS             11514..11927
                     /locus_tag="IVD64_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6148290.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            11940..12326
                     /locus_tag="IVD64_001025"
     CDS             11940..12326
                     /locus_tag="IVD64_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148291.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            12818..12890
                     /locus_tag="IVD64_001026"
     tRNA            12818..12890
                     /locus_tag="IVD64_001026"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12851..12853,aa:Lys,seq:ttt)
     gene            13276..13536
                     /locus_tag="IVD64_001027"
     CDS             13276..13536
                     /locus_tag="IVD64_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148292.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            14141..14974
                     /locus_tag="IVD64_001028"
     CDS             14141..14974
                     /locus_tag="IVD64_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP6148293.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            14967..16382
                     /locus_tag="IVD64_001029"
     CDS             14967..16382
                     /locus_tag="IVD64_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP6148294.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            16394..17923
                     /locus_tag="IVD64_001030"
     CDS             16394..17923
                     /locus_tag="IVD64_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6148295.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            18015..18893
                     /locus_tag="IVD64_001031"
     CDS             18015..18893
                     /locus_tag="IVD64_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP6148296.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            18895..19212
                     /locus_tag="IVD64_001032"
     CDS             18895..19212
                     /locus_tag="IVD64_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148297.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            19261..19962
                     /locus_tag="IVD64_001033"
     CDS             19261..19962
                     /locus_tag="IVD64_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP6148298.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            19977..20867
                     /locus_tag="IVD64_001034"
     CDS             19977..20867
                     /locus_tag="IVD64_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP6148299.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            20890..21105
                     /locus_tag="IVD64_001035"
     CDS             20890..21105
                     /locus_tag="IVD64_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148300.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            21117..21458
                     /locus_tag="IVD64_001036"
     CDS             21117..21458
                     /locus_tag="IVD64_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148301.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            21458..21829
                     /locus_tag="IVD64_001037"
     CDS             21458..21829
                     /locus_tag="IVD64_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148302.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            21822..22217
                     /locus_tag="IVD64_001038"
     CDS             21822..22217
                     /locus_tag="IVD64_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP6148303.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            22219..22596
                     /locus_tag="IVD64_001039"
     CDS             22219..22596
                     /locus_tag="IVD64_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148304.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            22597..23205
                     /locus_tag="IVD64_001040"
     CDS             22597..23205
                     /locus_tag="IVD64_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6148305.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            23205..23546
                     /locus_tag="IVD64_001041"
     CDS             23205..23546
                     /locus_tag="IVD64_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148306.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            23788..26577
                     /locus_tag="IVD64_001042"
     CDS             23788..26577
                     /locus_tag="IVD64_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP6148307.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            26567..27307
                     /locus_tag="IVD64_001043"
     CDS             26567..27307
                     /locus_tag="IVD64_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6148308.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            27304..>29141
                     /locus_tag="IVD64_001044"
     CDS             27304..>29141
                     /locus_tag="IVD64_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148309.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT        10360 a         4251 c         5923 g         8607 t
ORIGIN      
        1 tttcttaatt caccaatgta ttttttgttc gtcaacatat ttttaacact ttgataatct
       61 ctaacgattc catactgaga gcctaaataa taaacagttt ttcttaaact accactagaa
      121 agaaagtagt tgaatatatc tatcacgata ggagcatcat catttattac taatttctta
      181 ttttctatag agtagcctaa cggagttttc ccgcttacta cttcaccttg tgcaatttta
      241 tttgccataa cagctttgat gcgttctgaa tcaatttggg cttccaattc agcaaaactc
      301 attacttgtg caatgaaagt tctgccatac gctgtagttg tatcataata ttgttgagaa
      361 acagcattcc aagaaacatt gtgtttttct aaaatttctt gagtgtttaa ataatgtcgt
      421 agatttctaa accatctatc taattttgta aataaaatta gatctatttt attttctttt
      481 acatcttgta atagtttttg aaattcatca cgatttattt tttgaccaga aataccaccg
      541 tctacatatt cattaactac tttcaaatcc ttgtgttgtt cgatatattc atagagggtt
      601 tctttttgtt ctctaagact atctccatgt ttggcttgtt gttcagttga tactctcata
      661 tataatgcta ctcgtttcat gttatctcct ttcgaatgta tgttcttatc gttgtaaaag
      721 aaaagcccga aggctaatct ttttactaca catctgttaa ttctaatgat cttctgtatt
      781 gttcagcttg agctagttta gtaaaagata cagttccgtt ataattttct aaaacaactt
      841 tttcaatttc atcaagagat actttaaaga attcttttcg ttgattaact ttattgactt
      901 cgttttttct aaaatgcgaa tgtagttgat tttctaatgt tggtgcatct tctgaaaata
      961 tcattgcatg aacatcaaat tcaaaaggta cagaagcgct actaagttcc ttaattctat
     1021 ccataggttc taaccgtcta gtcattccta ttttatagac atcttcaccg aaagatccga
     1081 tattactgat gacataaaca tagccagctc tagtattaag ttctctttgc aatacatctt
     1141 ctttgacagt ttctagttct cttaatttct cttccagttc tttaatttta tcagcataga
     1201 gttctttttc aacatcagca ctagcttttt gcatgtatgc cattaactta gttatctctg
     1261 agttaaactg tcgttcttct ttatcaatgc gtttcttttc acgttctatt tcacgtctta
     1321 ccttttcttc ttcaatcatt tgttctttta ttgctttttg ctgttctttt tcatcgttaa
     1381 ctttaactat gtatgaatag ttgagttcta attcttgaag ttttaattca agtaattctt
     1441 ttttgaattc tgcttcatcg tttttaaaaa gcttgtttat tttatcaaaa gttcttataa
     1501 ttttttctct agttgaatct acgttagaaa tagtaagttt attgagaagt ccagttattt
     1561 ctgattctaa tggtataagc attttctttt taatattatt agcttctcta ttattcaaat
     1621 aagaaggaat attaattgca ccgtctaaaa taaaatcctt ttctttaatc tttaataaag
     1681 aaagtttgtt ttttatttcg gatgaagaag tttcatctat attcggaata attactgatt
     1741 tatagatagt gaccagagat gtgtcatatt tttgattaat ttcatcgtct aaccgctggc
     1801 tttcagcatt aagaatatca attttgtttt ctagcgttga caagttttta ttcaatttac
     1861 ttatgttttt ctctttttca gatataaacg tttccctagc atttaattct tgagaatatt
     1921 cctttacctt actctctaag gcagaagttt tatcttttaa ttttttagct tcagataatt
     1981 tgctactacc cataattatt aaaactattc caactaaaaa caatagtact cccattcaaa
     2041 acgcctcgct tcttttataa aataattgtc aaaatcatat aaacccttac cgaagttgac
     2101 ggctccggca tgggaatttt tttaaaatac aacttttccg ataattctaa tctgttctgg
     2161 cgatacaatt ctatcttcgt ataagtcgtt tatagaacgt aaaatgattg tttgattttc
     2221 gtaatcttta tatatctctt tacatgtaac tccgtcacca cctaattcca caatagcaat
     2281 ttccccatct tctacgtccg gttgttcttt aacgaatacg aattgtccat tcctgataac
     2341 aggttccatt gaatcgccta taataggtac tgcgaagtca gcagcacttg gaactctctc
     2401 aaattcttcc tcttcgacaa ccgtatcacc gtaagtaagt tctgttggat tggcagccga
     2461 agccgaattg tgaacagttg ggattttttc ttttttagga aaatctagga cattttcttg
     2521 tttttgttct tctaatcgat tacttgcaaa ttcataggta gcaactttat tttcgtcgtt
     2581 aagctgatta tatataggaa ggatattata tatctgatta gatatagata aatcaatatt
     2641 catcaaatca tctatatcaa cattgaatat cttagctatc tcatttaagg tttttaattt
     2701 tggtgtatat tttccttttt cccattcgct tattgaggat gaactttttc ttcctagttt
     2761 ttcggctagt tcttgttgat caatattata cttttctcta agaaacttta gattttcagc
     2821 gaacatagta acacctcctt gagttaattg taacaccgtt tcggaaaaaa tgtaacaggt
     2881 ttcttagaaa atattttagt gtttttaaag aagtatttac gcaaaataga aatcgatttc
     2941 aggaaaactg aaatatgcac ttgacttcgg aaaaaccgaa gtatataatt tagtcagaaa
     3001 gttaaagaga ggaggcgaat aaaatatgga taccgcgaag aaagaaaaat atacattgag
     3061 acaattaaga gctttaaagg gttattcaaa agaagaactg tcaaggaaaa gcaaagtgac
     3121 atcaagaaca atatttattt acgaaaacga tgtagataaa atgaggaacg gaaagtacgc
     3181 cactctagaa aaaattgcta aggcattggg tgttagagtt tccgatattt ttttagaccc
     3241 tgattcggaa aaaccgaaat caataacagg tgaaacaaaa caagtcagct aggaggtgtg
     3301 aatagtggaa gagaagaata taaaagtacc gactcttgaa gaatatacaa aaattgttgt
     3361 cgaaacagac gagcagaacc cgaaaacaat cgcagttatt acggcgaatg atattgaatc
     3421 atgcgaaggt tttcgagttc gaatgacccc tagatatgat taatgttctt tatcacgtgg
     3481 aggtatagga tcgtttccaa aactatcttt tgattgtatt tgtccattct ttttttggac
     3541 aataaactct gattgctgtt ttttagaaat ctttttacca tactcaacag catctgattt
     3601 attatcaaat agttttgtat cttcggaatt accttcacca cgtacagccc atttgccatt
     3661 tctaggagat acccattgat ttttacccat aacaattcac ctcgctttca agttaattct
     3721 attaagaggt gaaaagaaaa caaactgatg gaggtgagat tgtgagtaac aagccaaaaa
     3781 tcactttgaa tggcacacct aatcttcaaa gattagcaga tgaaatggct agaatacgtt
     3841 ctagacattg gggagttact gtggtggcaa ccataattga acaaaaagaa aagcccacac
     3901 agccggcaag caagtagtgg gcaccataga acaataatat tatatcacac actcgccact
     3961 tccgaaaaga ggagttgctg ctctcctttc aagtttgcca aagtccacgt ttcttcattt
     4021 tggcaactcc tcttcttgga ggtggtgacg gcgagagacg aaggagggat gacatgaagg
     4081 aagaatcaaa aatagggatt ttgaatgaga acaattacac aagattaatt attgaaacag
     4141 aagaagggga aaaagtagca gaagttacat tgaccgatgc aactcctgct gacggttatc
     4201 gtattcgatt aactcctaaa tatgattaac cttttggtgg gtgtggatcg tttccgtgac
     4261 tatcacggct ttgaattttt ccatctctgc catgaataaa tagctcagat ccttgatttt
     4321 tagaaatctc tctagcgatt tttactgctt ctgattttgt attagtatgt gcagtcgctt
     4381 tagaatttcc agcacctttg acattccaac caccattgga attaggtaca acatgttgat
     4441 tttttcccat agttttcacc tccatttcta tagaaatatt ttatcaaata gaaatggaga
     4501 aattcaataa acgaaaggag ggatcaaatg acattacaag agcatcaaca cttgatgcga
     4561 aaactaaatc aggaatacca cgaggaaatg aaacctagat tgctagggga acaatggcgt
     4621 gaacgccaga agaagtggtt agaaataaaa aagtgactca gccgtccaaa gcaatgagtc
     4681 acaaaccaaa aattatacct aaggagattt tatcacatga ataagaaaat tgaaaatctt
     4741 atcgaagaat tgaaacgtga atgccaaaag caaggtgttt ctattatttg tacagcccaa
     4801 aaagaagggg aacttaaaag tctcgtttat ggtgagacaa cagaaatttt actttgtctt
     4861 gctatgcaag aagaacattt agatgaaaat ctcccattgt cagcacatat tatgcgtaga
     4921 attgctgtgg atgcttacga acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat
     4981 acttttgtta ttaataacaa ggaagattta gctgatgtga tgactcgtat tctcaagggg
     5041 gaatttcaat gatgaatgaa aaaacaattg atctactcat tattttattt ttcacgttag
     5101 tgattgtagc gaaaagttat gtaatctttg gcttgttatt cgcaattacc atgcttgtgc
     5161 tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg agttagcaaa tcttgacaac
     5221 tatttaactg atcctgatta tgctaaacca ccatatgaag cgccaattga tgaggaggat
     5281 gaagatgagt aaatctacct tagaaatgag ccatcaagaa tggctcgaag accgcaaaag
     5341 aggtattggt ggctcagatg ttgcaaccgt acttggatta aacaaataca aatctcctta
     5401 tcaattgtgg cttgagaaaa cgggtcaaat tgaattgaaa gatttagaaa gcgaaccagc
     5461 ttattggggt aatgttttag aagaagtggt tgctaaagag tttcaggaac gtacaggcaa
     5521 aaaagtacgc agaagaaacc aagtatttga acatccatta catccatttt taagagcaaa
     5581 tattgatcgg gaagtagtgg gagaaaatgc cattctggaa tgtaaaacag ccaatcaatt
     5641 tctcggaaaa gagtgggaag gtgaagaggt accgcttagc tatctctgtc aagttcagca
     5701 ttacatgaac gttttaaaca aagactattg ttatatcgct gttttgatcg gtggacaaaa
     5761 attcatttgg aagcggattg aacgagatca agagctgatc gatacaatca ctgaacaatt
     5821 agtagagttt tgggaaacga acgttcttgg aggtatcgag cctgtaattg acggaagtgg
     5881 agcgactgct gacttcttaa aagaaaaata tgcagatgta gaagaaaatc aaacagctct
     5941 accatcacgt tttgatgaac ttatcgagca aaaaagagaa ctcaagcgga cgaaaaaaga
     6001 aattgaatca tctattcgtc aagtagacaa cgagatcatc agtgagctag gtaaacgtga
     6061 agccagtatc ggtatcacac aaagaaacat tatcagctgg aaacttgttc gtacaagacg
     6121 gatgaacacg aagaaactag cagagaaata tccagatgtc gcaaatgatg aagagattta
     6181 taacgttact gaatcaagaa ggctaaccga aaaggagatc aaataatatg gcaacaaatg
     6241 aatcgttaaa aaatcaattg gcagaaaagc cacagaaaca agttgcacca ggacagttag
     6301 ggcttaaagc tctaatgaat acaccaacaa tgagaaagaa atttgaagaa gtacttcatg
     6361 acaatgctaa tgcttttatg tcgaatgtta tgactcttgt atctaatgac agttatcttg
     6421 cagatagtga accgatgtct atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg
     6481 ggctagataa gaatttaggt tatgcatatt tagttccatt caatagtaaa aacaagcaaa
     6541 caggaaaatg ggaaaagaaa gctcaattca tgcttggcta taaaggatat atccaattag
     6601 cccaacgatc aggtaaatac aaagcattaa atgtgatcga agtttacgaa ggagaactaa
     6661 aaagctggaa ccgactgaca gaagagtttg agtttgatcc aaatggtaga acgtctgatg
     6721 aagtcattgg atatgttggc tatttcgagt tactgaatgg attcaaaaaa actgtctatt
     6781 ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa taaagatcgc gataagacaa
     6841 agttaagtgg tgtgtgggca tctgattaca atgcaatggc acgaaaaact gttttgagaa
     6901 atcttctttc taaatgggga atcttgtcca tcgaaatgca agaagccacc acatcggatg
     6961 agagagtcca aagggttcaa gaagacggca gcattattgc tgaaacagaa gttgaagaag
     7021 atattcctga aagaaaagaa gcagaggtta tatctgaaga aaatgaagat gtacaaactg
     7081 gattatttga tgcatctaat ccgccgttaa acaaataatg agggagcttt ctcccttgct
     7141 tttctagagg agaaatacga atgaacacag gatatgtcaa attacatcga aaagtgatga
     7201 attcattcgt ttggaccaat ccttatatgt ataaattatg gagcttgtgt ttgatgaaag
     7261 cgagtcatga gaatcgcaag atacttttta atggaaaaga gatacaagtg aacagcggag
     7321 aattcgtcac agggcgcgat gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac
     7381 atcaagtgaa cagcggttct gtatggagat ggctaaaaca atttgaaaaa aacggaatgt
     7441 tgaacatcaa atcaactacg aaatacagcg ttatatcaat aaaaaactgg tctttgtacc
     7501 aaagcagtga acaacaaatg aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa
     7561 acaagaatga aaagaattat aagaatgaaa agaataataa aacatcatcg tcgcaaccac
     7621 gcaaaaagcg tgtttacgac accgactcag tttactacat tctcgcggag gagttattca
     7681 aacagatttg tcagaatcag gaaatcaaaa agccgaatct gcaaagttgg gcagataaca
     7741 ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca ggtacgggga atgattgaat
     7801 ggagtcagca caatgtgttt tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa
     7861 aatacgacac aatggcagcg caagcaaatc gtgattataa aacaaaacaa actaaaacgc
     7921 ttgaatacga gaaatttagc acagatgagt tgcctatttg agaggaggcg taggcatgga
     7981 aactgttggg gaaatcatgg aaaagctgat acagaaagtg cttgtccaac gtggcgaatg
     8041 tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag aaccctgatg gctcagaacg
     8101 ttgtaagcca acctgtatgc aatgtgggta taaagcattg cgagttcagg aagacttaca
     8161 aacagaacga atttacaacg agagcctgaa agcaagagca atcaattttt tcaaaggtgg
     8221 ttctgttgtg cctaatcaag cgttgtttga ttgcacattg cagaattatc aaattgtcga
     8281 tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt gttaattcag tcttgttagg
     8341 aaatccaagt caccttgttt taactggaaa acaaggaaca ggtaaaagtc acctagcgat
     8401 ggcagcggct tgggaagtct tgaagcgatc aaactacgat aaaaaaatct tatttatcgg
     8461 gttacaggaa atgctggatc aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac
     8521 gattgaggga tcgttgattg cagatatcaa aacagcggat ttagtcatca ttgatgatat
     8581 cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg tttggcataa acacgctaaa
     8641 ttcgttcttg gatgcacgac agaacctagc aacgattatc acaacgaact tgcttggtga
     8701 agaactgaaa aaagcttacg gtacgagaac aatatcaaga atgtttgtca actctgatgg
     8761 atttacgatg gtattttctc aaacagcaga caagcgcata aaaccagtca aaggagcgat
     8821 cgcatgaatt tgactaaaca agaaaaaaga atctgtcgtc agataatgtt gcaaattata
     8881 ggtgatcgtg acatggacga attatctcaa caagagagtc gaatggttcg gaagataagt
     8941 aagctaatcg gtgcagatac gatggatcga tccaagccat tacccaaact gaattttgaa
     9001 tcatttacgt ttgagaaata tagccatctg agagaggtag gctatagtgt aaatgctatt
     9061 cgaaaagcac ttggggtagg aagtagtgta tgggcaaaat ggcggaaaga aaatgtaaat
     9121 gaggtaaatc aaaatgatta agctaatagg caaagagacg ctcaaggttt actatgtagc
     9181 ggaaacaaag gcagaagtca gccgttggct attgaaaatg tttaataaga ctagagccaa
     9241 tgcttcagta agtcagaaag caagtgtatc agaaacaggc atgcctgaag caatgtggat
     9301 ttcaaggagg aactgagatg gcaatgatac cgaagtttag agcgtgggat agagaaaaag
     9361 acagaatgat ttatcctagc acgacaggag tttgttttga aatgaatgat gatggtataa
     9421 gtgtgcttga tattgatgta gattatccca atgactatgg ttttcccaaa atagcttcaa
     9481 tcctcatgca atctaccact ctgaaggata agaatggtgt ggagatattt gagggggata
     9541 tagcgcttat cactcataaa tcaacgaact acgccgatac ttattggcac agttacgtag
     9601 aagtttttag agcaaaaaac ggagcctaca gaattcgcgg tgaacacatc tacgaaacag
     9661 aattatatag taatagaaaa caattgacgg tagcggggaa tgtttataat tcaccagaac
     9721 ttttgaagag agatcgattt atgactacta ccgaaaatcc agagttattg aaggaggaac
     9781 agcgatgaat aaacaggaat tgattgataa atatactgca gaaatatcta gattaagacc
     9841 ttattgccct aatagacatt taattagcga tcaacttaaa tatggtcttt ataaagaaat
     9901 cctagaagat ttaaaacaac tagacgaacc gcagaaacca gcggtgccta agtttgtggc
     9961 ggaatggata gaatacgcta aaaagaaagg cgatagtcta gctatttcat tcaagccgtg
    10021 gaacctctac ggtgttgagt atagcaagac tgatagatgg attgaagata atcaaggaac
    10081 gtttgctcgt gcttggctaa ttggctacga ggtcgagaaa gagccgttgt attatgtgaa
    10141 gttgccagtt gtgtatttta atcattggga tttagagacg tatctaatga aagatgatag
    10201 aggaaatata acaattgcag acaacaacga ttttgatgat atgaaattta cggaatcaga
    10261 aataaaagca attgatgaaa gatactggcc atttgctgtg ccagtggaag aggtggcgga
    10321 aggatgagca atcttaaagt tggagatatt gttttttaca aaggtgacga aggcatcatc
    10381 cgagaagtta tgacggatgg aaaagaaaat ttttataggg tagatgtcag cagaaaatct
    10441 atactctacc tgtatgaaga cgaattagat ttaggtactc aaaaactcaa cgacaaccag
    10501 aaaatggtgt ttgattatct taaatcaaac gttgaacaag gtggcaaatc agttatgtat
    10561 tcgatattcc ttctaggtga ttgggactct cgaattggag cagcaagaaa cgttgatata
    10621 gcttactgtg gattaaatgg taagcaggaa gcagaagtcc tatcagcatt cgctcaatgg
    10681 ggattagaac aggaggaagc ggaatgaaat acgaaatacc actaagtgaa gcagacgtcc
    10741 agtcaattat taacggtcgg gaggttaata aaaaacttcc tgatggtact gaattagtca
    10801 tcagacaaag ttatttgaaa gatatggcag ctccagtatt aattgatcgt tttaacgtga
    10861 ctgattctgt ggtagagaac catttaaaag aatttcgatc aagtatagac gacactttca
    10921 gattagggag ttgattgaca atgaacaaca gacatcgcag aataacaaaa ctaagaaaac
    10981 aggaactgaa tgtactaaag acaaagtttg aaaaagaata tggaatttca gcagaagaaa
    11041 catataaagt ggcaagtcag tgtgttgctg atgcgagtaa tgctattcgt aagtttggga
    11101 tttcgatatt aaatgatgat cgtaaatggg aggaaatgag atgaaactaa aagacggatt
    11161 ttacgctagt agtcacggca tcggcggttt aatgctagat atgccgacaa agaatcctaa
    11221 aacacgtaag aaaccaaaag tcaaagtcgg tgacatggtt cgctgcgaag cagaagggtt
    11281 catctatcca tttcgtggat atgtagaaca cgtctataat cactcagcaa tcattcgtat
    11341 tgaaaacacg atggaatgtg ataaatggac agctaaaagc aaagagaatt tagcagtggt
    11401 gagattggtg gatattgaac taatcaatga caaataaaaa agccggatcg ctccgactga
    11461 ttcaataaat tccacaagtt tattatatca cataaaaggg gcggtttgac ttgatgcaat
    11521 tgttacgaga ggtagctttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
    11581 ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg ccgattatta
    11641 cggacatgcc gaaggcaccg aagcacggca ataaggcaga agacgcgatc attcagatga
    11701 tggatataga agcagagaga gacgcgattt tagcggcttt gatggcgctt agtctgatta
    11761 gtcgtcagat actctactac agcttttgtg tgccagatag cttctcaaac tacaggatta
    11821 gccgtgaagt gggttattca gaaagaagca tacaacggat gaagtcggaa gctctgatag
    11881 agtttgcaga agcatataaa catgggaaaa taattgctta taaataggag gactatataa
    11941 tgtggaaaga ttatgtatca ttgaaagagt tgaaaaaaga tcttattttc aaaaaaatcg
    12001 tagaatggtc agagagtgaa ttaattttag aagatggaac caaaatggaa gttgtatgta
    12061 gcgaatcaga ttgctgcgca tgggccgaag gtgaattcaa aaatgtaaag cttgatgcag
    12121 tgattacaga tataaagatt tttgataaag gtaaccgtct ctataatggc gatggacata
    12181 gttcttatgc cgaagtcgtt gtttatcata atcgaaatga gattgctaaa gcggaatgta
    12241 cagcaaacga tgggaatgga ggctactatt acagtgtctg tgctttaaaa gtcaaagaaa
    12301 aactttgtgt agtgactgat gcataaaaaa tggcggtttt ttggcggaat gatggcggtt
    12361 tttagctatt taccagtgat attatggtag tgtcgaaaga ttagtgatag gtctgagaca
    12421 aaataaaatg taagggagga aatctccctc atcgtttaat taagcttcga tagacagcag
    12481 cggaaatatt aagaataagg atgtgaattt taactccttc taaattgttc ttattatcta
    12541 tcatccgttg ctgtctattg ttattatgtc actgtggcgg aaagggtaga cgcttaaaaa
    12601 taaggtcaat acgtcgaggg atagccttaa cgttttatga tttgaccatg caaggttcga
    12661 ttccttgcca gcgacttaga cgaaaggtag cttaaatatt aaccacatca acatcgtcag
    12721 cgagttgata aaggtaacta gcagactcta ggatttaagc cagtcttacc gattagtctg
    12781 ttatagtagc aagcttgcta gaggtagctc ctctggttga cgtgcagctc agttggtaga
    12841 gcgcctgact tttaatcagg aggtcgccgg ttcgaatccg gtcacgttaa taaagaacct
    12901 acggaaacaa tcaatcttat cggatgccga ttggttggct gaattaagca gtgtgtctgt
    12961 gaatcagtta ataggcataa aatactagca cagacgtgtg ccactctcag gtgtaggtta
    13021 ggagagaaac attagcaacc gagggttgga aatgggcgct caaagtacac gagcaaggcg
    13081 aggtcgatag taatcgatgg aatcggtgta ggttgctatt acataattga ttgggttaga
    13141 ttgagttttg ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac
    13201 gtccctgcct gtgcattaca tattagatca ctcgttgagt ggtcttttta ttttagaaaa
    13261 gagaggattt tgaaaatgaa tcatgagaag tttatcgaaa aatgcaaggc tattgtacgt
    13321 gaaagaattg agaatgagat tgctgaccta agtggagcag tacctgagtt tagtgttttc
    13381 atagtctggt catgcaaaac actacagaat agcaaagcat tagtcagcgc tagcttaaaa
    13441 ggagcaccgt attttgaaat tacgttgaac ggggacaaag gtgaaatcta tgtagatact
    13501 tatctcaaaa aatcaaatga atgtatcaaa gtctagcagg tgctaggctt tttctttaca
    13561 taaaggagga atgatctcat gaaacaatat accgctaaag atttcgagga aatgaagcga
    13621 ttaaagaagg actatgaaga agttgatatg gagctaactg ttggagtcat tcaacgaaga
    13681 ctgcgggtcg gattagagac agcaaaggct atttacaatg atctaaatgc tattgaagag
    13741 aagaatggct aatgaggaac tactggtata tatcgctaac taatgaatat cctcgaacca
    13801 ttgatgattg ttcagtgcgt gttgtgcgtt ctgtacaaat caaagggaag tactctattg
    13861 tcgaaatggg gagagaagca gaaccatgtg agattgatgc gtgcaagctc gtttattgcg
    13921 gtcatggttt ctatgatgaa ccaaacattc aaaataatat taacaagaat ttgagggatt
    13981 agaatgcaag aaatggctta aaaatctcca atcaggacaa atcaagttag acaaagtttc
    14041 cgatttaaag atattaattg aagcagatct aatgttgaaa gatattgaaa attagaaaac
    14101 aaaactcaac ctaagaagat tgcgaggtgg tgtgtattga atggcaagac aacgtgatcc
    14161 aagacgtgat gaagccaaaa gaatttggtt agaatccaac ggagaaaagc agttaaagga
    14221 aattgcatct gaattaaatg tttcagattc tcaggttaga aaatggaaat cgcaagacaa
    14281 atggagcgct gaattgaaaa gtaacgttac caatggcaaa agtaacgtta ctaatcaagg
    14341 tggcgctcct attggtaatc aaaatgctaa aggtaacaaa ggaaatagcc gagcttctcc
    14401 gccagtgggt aataaaaacg ctttgaaaac aggcgagtat gaaaccatat tttttgagac
    14461 actaagtgat gaagagaagg acatctattc tagtctgaat gataatcctt cttttgtttt
    14521 gtctgaagaa atacgtctac ttaagataag acaatttcgt atgatgaaga gaatccaaca
    14581 agctgaagct ggactaaatg atgaagaagt cgaacgattg cagcagctaa gaaagattaa
    14641 aaatccgatt gaaaaaaatg gtaaaaagct agaaatcaag cgtgaggtta tgcaagatgt
    14701 gcagattagc agaaaaaaac atcgcaaaat tgatgatatt ctttcaattg aagattcatt
    14761 gactcggatt agcaaccagt tagctaaagc catcaagcaa atgaatgaac tttatatgaa
    14821 tgaatacaga actgatttaa ttaaagctca gactgataag atccaagctg agacaaatga
    14881 aattggcgga aataattcag gtgaagaaat agaagaatgg aaacaggcag ttttaaatgc
    14941 cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt tattcctttt gctgatattg
    15001 gttctgccat tgattattac tatgataaac cagtagcttt ttgccaagat attttgcatt
    15061 tgaatcctga tgaatggcaa gaaaatgttt taaatgattt agctgaattt tcaaaggttt
    15121 ctgttcgttc tggtcaagga gttggaaaaa cagcattaga agcaggagca atactttggt
    15181 tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc tccgacaatg aagcaacttt
    15241 acgatgtact ttgggcagag gtagctaaat ggttaaatga tagcttgatc aaaaacttac
    15301 tgaagtggac aaagaccaaa atttatatgg ttggtgattc agagcgttgg tttgctacgg
    15361 ctagaacagc gactaaacca gaaaatatgc aaggttttca cgaggaccat atgttgattg
    15421 tggtagatga agcttctggt gtgtctgatc caattatgga agctattctt ggtacgctat
    15481 caggttttga taataagctg ttgatgtgtg gaaaccccaa taatattgaa ggtgtttttt
    15541 acgattccca caattcagac cgtgataaat acagagttca taaagtatca agctatgata
    15601 gtaaacgtac aaacaaagac aatatagaaa tgattcttaa aaaatatgga aaagaaagtg
    15661 atgttgctcg tgtccgtatt tttggagaat ttcccaaagg tgcgttggat tcatttatca
    15721 gtcttgaaac ggttgaattg gctacagaaa aacaaattag tgattcttta gtcaataaaa
    15781 caacggttgc tcatattggt gttgacgtag ctcgatatgg tgatgattct acgattctct
    15841 ttcctagaat tgctaccagg gcattggagt atgagaagta ttcaaaacgt agcaccatgg
    15901 aaacaacagg atatgtcatc aacatggcca agaatctaat gagtcaatat ccgagtattg
    15961 ataaagtgat gattaaagtc gatgacactg gtgtcggagg tggtgtaacc gaccgcctag
    16021 aagaacttat agaagacaaa cattatcctt ttgaggtgtt tggagtgaat aacggttcaa
    16081 catcagaaga cgatttttac gataatttag gtactcaact atggggaaac atcaaggaaa
    16141 tgttagaaga aaatatgaca gcaaatctta acggagaaca gcctgttatt gaattgcctt
    16201 ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt caaaatgaca agtagaagtc
    16261 gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa tattggtagt cccgatattg
    16321 ctgacgcact ggctttagcg ttttatgagc caccaagtca ctatcaattt attcaatttt
    16381 aggaggtgag cttttgatta gtacagtatt atctttagag agatataaga agctacgtgt
    16441 taaatatgca acacaaattg aagatggaat gtttgatccg aatggtttta tagaagatat
    16501 gaaaccattt tttgctgatc gcgaaagaaa atatctagct tatactagcg aaaagaatga
    16561 aatagataat agaccaaagc ctaacactga tattgtaaaa gttaataata aacttcatgc
    16621 tggtatgtat tcaattgtcg tagaccaagc agtcaatcat tttactggta tacctatcaa
    16681 atgggattac gatgtatcgg aacaaaaaag aacactcatt caaagattaa aagataaatt
    16741 cttaaaaaat gatatagaac ttccgacagt tccagaagct tttaacaaat taacaagcaa
    16801 tcttgattcc atgagattcg caatgcttga ttctgaaact gcaacttttc aaggagcttg
    16861 tggagtagct ttccgattgt tagagcctgt ggaagaagat gatggttgga agttaagagc
    16921 aagtaatatt gaaccttgga gagcagaaag atatggaaat gccggaatct atatcaaaga
    16981 aaagtatgac tcttaccaga aaaaattttt tcaagaaatg aaagttatga caagaaataa
    17041 aattctgaca tatgcttgct atggtgattt gaattttgtt acaagcggaa cgttcaaaaa
    17101 aattgatgag accgataacc ctttggggac gattaccttg gcagaattta aaaataatac
    17161 gaatcgttat tgtgattttg aagtagctga ggaaattgga gatgcaattg atcgtgcttt
    17221 gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg tacatgcttg ttactggcac
    17281 aacaatgagc aaaggcactg ctaaagaaat gatgaatcag ctagggatta tcaacttaaa
    17341 agaccctact gcaaaagctg aatatgtcac aaaaaattta gcaaaagatt tcaatgaata
    17401 tcatatggat ctattgaaaa aacagtttta caccatttgt aaagcaatcg attttaacga
    17461 tgaggtgttt aaatcaaaca gttctggaga agctcgcaag tggcaaatca ttagcctaga
    17521 agcgaaaaca aatactaaag agcaatattt tagagaagga ttgaaagaat gcgcagaaac
    17581 aattgctgct tttcttaagt ttcatgacaa agtagaaatt gagcctgaaa aaattatttt
    17641 tactttctct agatctttgc caactgattt aagttattta gctgaagctt tacctaaact
    17701 tgcaccatac gtatccaaac gaactattca gagccaaatt ccatttgtaa cagatgttga
    17761 ttatgaaaat gaaatgatgg aattggaaag tggaagtgct tatccagata gtgaatacaa
    17821 ttttggcgga ggtggcaata gtgacgatag aaacgaagta ttggaccaaa cgtcgagaac
    17881 tggaggatca agcaaggctc aaacaagaaa atcagacact taaaaaatta actagtgtat
    17941 ttcctgaagc actgaaagag atacaagcaa aactattatc acaagctgac ttacacaata
    18001 tcacttatcc agaaatgatg gagttttata gtacaagtaa tcagaaaaaa tatcgtgaat
    18061 atgtggaaaa aaattataag tcattaaaaa tgtatgatgc aaaatacaaa gagtttatcg
    18121 atgaattttt tccaccattt gactatgcaa aagtcaatcg cttattacaa atacgatcag
    18181 atgtatttaa aattcttgca gaatatgcga tggatgcaga tgtgaatcaa tatttttctg
    18241 atcgcttaga ggaaattctt caaagaacat attcttctaa tgctaatgtt tttgttcaac
    18301 ttttaaacgt tgatatacca aattatttac cagaaaatga aattaaacat tatttgaact
    18361 atccatggtc aggaaagaca ttttcaagaa gactttgggg aaatatttca tcgcttgaac
    18421 agaaactctc taatgctatt gtaaaaagtg ttgctagtgg agaaggcgtt atacacgcat
    18481 taaacaccat gagattagac tcagaaattt gtgacatgtt taagttagaa gaatcaaagt
    18541 ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc aaaatttgca caagatggta
    18601 ttgaaaaatc atatttagaa acaggtattg aggaatacaa cgtattgact gcaaaagatg
    18661 agagagtttg ccggatttgt ggaggaaagg caagtaagaa tccctataaa ctgaaagatg
    18721 ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg tagatgtacg gatgttccta
    18781 atttaccaaa attaggaaag gatattgatg aagaatatga ccgtttattt ggcgatttat
    18841 tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag tagaatgaaa
    18901 gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga agcctacaaa
    18961 acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa tggcaattat
    19021 gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc agttgataag
    19081 aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt aaaagaaaca
    19141 gttgattggt atgaaaagat gggatgtatt gttgttagaa ctgactacaa agaaggaaag
    19201 tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc aatactgggc
    19261 ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg tgaaaacgaa
    19321 tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat taatgccaat gaatttgcaa
    19381 tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc ttttgtggaa
    19441 tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc cgacaaacaa
    19501 ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat caaggaatat
    19561 gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact acaagctgaa
    19621 tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt tgcagaacaa
    19681 attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt tatgctaaat
    19741 aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt tacaagtgtc
    19801 ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat gaagtctgca
    19861 tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac attaacaaat
    19921 ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga aaaattatga
    19981 aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt aatgctatgc
    20041 aagtaccaaa tacacctttt ttaagctatt tatttggtgc tggaaaaaca gagccagcaa
    20101 actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct tctgaaaaac
    20161 ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac aactatactg
    20221 aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat gtgaatggag
    20281 tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa attgatttga
    20341 accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt cgtcgaatga
    20401 atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact gcgggtgcag
    20461 taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt tatatgggag
    20521 aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag ttagatggaa
    20581 aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa attggaaata
    20641 tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca aaaggaacaa
    20701 ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg agagcagaag
    20761 aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac tctcttgaat
    20821 acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag ggagttagta
    20881 ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa aaaagatgaa
    20941 gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca tcctaaaact
    21001 cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga tgataaagaa
    21061 gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt gatctgatgg
    21121 atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca gatttgagtg
    21181 aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca tacaacactt
    21241 gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg ttgtataaaa
    21301 taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat acaacgatta
    21361 gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt gatttgatac
    21421 gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag atattttaga
    21481 aactacttat gaagatagtt gtattattga aagactgacg gacattgaag attctaatac
    21541 aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt gcgctctttc
    21601 acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa atgtttcaac
    21661 tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag atcgaataac
    21721 aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc ctttttacta
    21781 tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat ctcaaatatg
    21841 aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt gctcaaaaag
    21901 aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct aaaccattaa
    21961 ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct agaaaactat
    22021 caagcggtcg aattgttgga gcagttataa acaatgaaca ttattttaaa tatgtagaag
    22081 aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta actcgtgcaa
    22141 cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca ataaaaatcg
    22201 ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag caaattaaaa
    22261 gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac taaagatttt
    22321 tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga tattcaaaat
    22381 gtttctttct taattgatat tgcattgatt gataataaac ctaatagaaa tttaattaat
    22441 gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga gcaaatatta
    22501 tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg ttttacatta
    22561 gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag aagtaaaacc
    22621 ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac agggaatttt
    22681 ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc aacagctaat
    22741 tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac agcttttggt
    22801 ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc tacaattact
    22861 ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc gcaagatggt
    22921 gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt cttttataaa
    22981 ggacagtttg ctctagattc agatgaaaca tcaacgattg aaggaagtaa agtaagtttt
    23041 actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata tatgtattca
    23101 gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt tacaaaagta
    23161 gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca aaatatcaaa
    23221 caacgattaa attaacgaaa aaagatgaag aaggtaaata tgagcaagta caatttaaat
    23281 ctgctgaatt tttaccagga acagttgtag aagatgctgc aggagttatg gaagaaatgc
    23341 aaacagcgac tgataaacaa tcagttaaaa aagctttaag tcgtgcttat tcatttattg
    23401 cagatacttt atttgaggga caatttactg gtgaagatta ttgcaaagga attgatgctc
    23461 gtgagattgc ctcattgaca ggaaaattat tgaagtctgt tactgcaggt tttgatgaaa
    23521 cttatacaga gacgaaaaaa aagtaagtga ggcgctcaag tcaccttcat ttaagtattc
    23581 gattacttac cgagaattag atataaagac gcaattactc gaagcaggtt ggacgttacc
    23641 agaaattgaa cgtactgact tggatgagtt aatgcgtctt tttgctttta gagatgcggt
    23701 aaaagagcat gaagatgttg agtactatga taatttcact caattttagg aggtgatact
    23761 ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt tactgccggt
    23821 atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag aaaaagtaga
    23881 aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt tctagctagc
    23941 ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat aagtggagcg
    24001 gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat gggatttagt
    24061 aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg tattcaagga
    24121 cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc ttctacaggt
    24181 gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct tggtttcggt
    24241 ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt ctcaaacgga
    24301 aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc tactctttct
    24361 gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt gtctcaaggt
    24421 aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa aggtggcgga
    24481 ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa aacctctata
    24541 caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg attaaataaa
    24601 gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt gggcagttca
    24661 atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc atttttaagt
    24721 aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat gccctttctt
    24781 gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat accagcaata
    24841 ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc attaaagatt
    24901 atgggggcaa tagctgctgc taattcattt gttttaatta ttggtgcagt cgttggtgca
    24961 atagctgttt tcggttactt tatggcaacc aatgaagagt ttagaaacaa ggttatatct
    25021 atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa ggactgggga
    25081 attgatactt ggaattctgc taaagaaatg gcatcaaatg cagttgaggg tgtcaaagat
    25141 gcttggtcag gaacaaagga atggttttct aacacctgga aaggtattaa ggatggggcc
    25201 acaggtttat ttgataaaac agtagagact tcaagaaatg cagtcgatag tgtaaaaaat
    25261 gcatggtcta atatgaagaa atggttttct gatacttggc aaagtataaa agattcagcc
    25321 agagaaaaat ggaatgaaat taaaggttct attatgtcgg ttgctggtcc attgattaca
    25381 ggtataaaga atgcattttt acatgttact ttttatctag agaccttatg gaataattta
    25441 gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc gccggttttg
    25501 tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaataatat gattgctgtt
    25561 tggaataata ttaaagaaag tgccataaat atttgggaat ctataaagaa tatatttgta
    25621 agttatttta caaatattta ttttgctgca ctcaatattt ggacagggtt caagcttaca
    25681 ttgataaata tttggaatga agtggtaaat caagctaaat cgatttggtt aaacataaag
    25741 tattttttta ttaatctttg gattgatata aaatattttg caattcagaa atggattgaa
    25801 ttgaagtttt ctattattca aacttggatc gatttaaaat ataatgccat tactacttgg
    25861 aataatatta aacagttttt caaagatact tggaaaaata ttaaagatac ggcatacaat
    25921 acgtggattt ctataaaaaa taccatgatt aatacatgga ataacattaa ggactctttc
    25981 tggaatattg ttactggaat tgttaattct gctgaaaatg catggacaaa tctaaaaaat
    26041 ggtgtttcaa atgctattaa tcgggtaaaa gaaatcttta attcgttaag ggaagtcaat
    26101 ttatttgaaa ttggcaagaa catcattgat ggacttataa atggtgtaaa agaaaagtgg
    26161 aatgctttga aagatactat caaaggtatt gcaggtaata tcaaaaattg gattaaagga
    26221 gctttagata ttcattctcc atcaagatgg atgcgtgata tggttggtaa aaatattgtt
    26281 caaggtatta tcgttggtat tgataaagag caaagtaagt tagatcaaac catgacaaat
    26341 ttagtaaaaa caccgtctgt tcaacctgta gtaaatggaa taagtcacct accaattaca
    26401 caaacaaagc aaaatatatc tgctagtgaa acaaaagaaa ttcatttaca tttaaatgtt
    26461 tatggagatt tacctgattc aatggttcgg aagattacca caaaaattaa aacagaattg
    26521 acaagacaaa tgaaacgaga tgctggtgca gtaggaggaa cattatatgc aacttaagag
    26581 aggacaattt tttattaatc aacattattc ttctgaattt aatgtgtata ttcaaaacag
    26641 gcctgcttct gtttcagcta gtcgagtaat tgaattgaga gagcgagaag gaaatgattc
    26701 tatcattatt gataaaggct attacagaaa tgtgactaga aagattgagt gttactataa
    26761 agcaccttca attgatgtgg tgcaggagtg ggaagatcga atcactgaat ggttagatat
    26821 gtcgtcttat agtgatttta ttctttacta tgatgaacaa tatatttatc aagctgtagt
    26881 aatagaagga cctgaattta aaggaacaag aaaaacagga aatattgtcc cttttgaatt
    26941 tacagtgagc attcgaccat ttaaggaaaa ttacaatggt agatttacta ttcgacaaac
    27001 agaaactttt gaaatttata acccagagaa gtattcttca aaaccgctta ttaaattgag
    27061 tggttctgga gatgcttctt tttatattaa taaggacaaa tatgatttga aatcgttgga
    27121 cagagaatta tttatagatt ctaaattaga agaggcttac cgaaaattag atggtaattt
    27181 agaacatcag gatcaagtca ctttattttt agattttcca tttttatatc ctggaaaaaa
    27241 tgaaattaaa tggacgaaaa atattcattc atttgaaata atgcctaggt ggtggagaaa
    27301 agtatgaaac caagaatata tagtcctact gaaacagatt tctcaacgaa tggcttagga
    27361 attttaaagg attgtacaag atgtgaaata tatgaagtag ctaatggaaa atatgaatta
    27421 gagttggatt atcctttagg aactagattt gatgaatatt ttgaaaatga ctatcaaata
    27481 aaagcaaagc caaacgatca agaagagtat catatctttt ttattgatga taaagatata
    27541 gatacttttt taaatacagt aactatttat gctcagagtc gtacaaatcg acttggaaga
    27601 cgggtagtca ctcttgcgga agtagactct aaaactggtc aagaagcgat atcaattatt
    27661 gaaaccaaaa tggataaaaa atctgacata cgactttatt ctgatattac ggccgtttct
    27721 agcacaatct ttgaagcgag aaatgtttta aattgtattg ctggtgaaca aggatcattg
    27781 cttcaatatt ggggtggaga aattaaacgt gaaccattta agctttcttt gttaaagcga
    27841 agaggtcgca ataatgttgg aacgattcgg tatggaaaag atatgtctgg tttaaaggtc
    27901 aaattagatt ggacaggtat aaaaacaaga cttattccat atgctgatcc tcaaagtgag
    27961 gtaggtacga ctaatcgaat ttatggttcg ccagtagata gtgcctatat taataactat
    28021 cctgatgtgt atacagagca tgttcagttt acagaagaac aaggagtaaa agacattaag
    28081 agcttaaata aaatagctaa aaattacttt aaaacgatca atcctggctg tgataagcct
    28141 aaagtttcta ttacggttga atttgataag ttgactgata gtgaagaagc gaaagaattt
    28201 gcgaagatta gaaattatgg tctgtttgat acatttaaaa tatatcataa aaaatataat
    28261 atttatcttg aatcaaaggt tagtggacta caatacgatt ctttatctga aaaaactttg
    28321 aaattagaag caggagacat tcaagttgct ttttatcagc aacaagctgt aactattcag
    28381 gataaattaa aagattatgc aacgaataat tatatgagtg attttaatga ttatgtttct
    28441 tcaatgatta caggtcaagg cagtgcagga ggatatgtaa gtttatggcc aaaagaaaaa
    28501 ccttccaaca tctttattat ggataatcca gatttagaaa aggcaagaga agtactaaga
    28561 atgaataaaa atgggattgc tttttctaag aaaggatggc gggggccttt tacttcagct
    28621 tggacattag atagtatatt taatgccaat tttatacaaa ctggattaat caaagcagat
    28681 atctttcaaa attcttttaa caagactgga gatgtattga aattagttaa tggactactc
    28741 caaatttgga acaacaaaaa gaaaataatg gagttaacta aaaaaggaat ggaattttgg
    28801 aattctaagg gttcaattgg aacgattgga acaactgatt ctgctggtaa tccttttcct
    28861 ggggcttcta ctccaacccc tattgaagat aattctttag ttattcgtac aaatggagac
    28921 ggcaaatata ttttgatttc tcctaaagta ggaaaaggat tagttttatt aggaaatggt
    28981 aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa aggaaaagaa
    29041 ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg tgggtatcct
    29101 agtgaattaa aaacagatgc agaaaagaga gcttggagaa t
//
LOCUS       DACBZN010000023        28735 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.023, whole genome shotgun sequence.
ACCESSION   DACBZN010000023 DACBZN010000000
VERSION     DACBZN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.023"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            293..2524
                     /gene="pflB"
                     /locus_tag="IVD64_001045"
     CDS             293..2524
                     /gene="pflB"
                     /locus_tag="IVD64_001045"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP6148310.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2768..3529
                     /gene="pflA"
                     /locus_tag="IVD64_001046"
     CDS             2768..3529
                     /gene="pflA"
                     /locus_tag="IVD64_001046"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP6148311.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3614..4540
                     /locus_tag="IVD64_001047"
     CDS             3614..4540
                     /locus_tag="IVD64_001047"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP6148312.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4722..5615
                     /locus_tag="IVD64_001048"
     CDS             4722..5615
                     /locus_tag="IVD64_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP6148313.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5749..5874
                     /locus_tag="IVD64_001049"
     CDS             5749..5874
                     /locus_tag="IVD64_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6148314.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(6008..6640)
                     /locus_tag="IVD64_001050"
     CDS             complement(6008..6640)
                     /locus_tag="IVD64_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148315.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6788..7330)
                     /locus_tag="IVD64_001051"
     CDS             complement(6788..7330)
                     /locus_tag="IVD64_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP6148316.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            7465..7854
                     /locus_tag="IVD64_001052"
     CDS             7465..7854
                     /locus_tag="IVD64_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148317.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            7858..8622
                     /locus_tag="IVD64_001053"
     CDS             7858..8622
                     /locus_tag="IVD64_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6148318.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPNIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            8751..9698
                     /locus_tag="IVD64_001054"
     CDS             8751..9698
                     /locus_tag="IVD64_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6148319.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            9828..10568
                     /locus_tag="IVD64_001055"
     CDS             9828..10568
                     /locus_tag="IVD64_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148320.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10859..11527)
                     /locus_tag="IVD64_001056"
     CDS             complement(10859..11527)
                     /locus_tag="IVD64_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6148321.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            11661..11948
                     /locus_tag="IVD64_001057"
     CDS             11661..11948
                     /locus_tag="IVD64_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148322.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            12364..13902
                     /locus_tag="IVD64_001058"
     CDS             12364..13902
                     /locus_tag="IVD64_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP6148323.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            13899..14864
                     /locus_tag="IVD64_001059"
     CDS             13899..14864
                     /locus_tag="IVD64_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148324.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            14851..16173
                     /locus_tag="IVD64_001060"
     CDS             14851..16173
                     /locus_tag="IVD64_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6148325.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      16236..16340
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16414..17157
                     /locus_tag="IVD64_001061"
     CDS             16414..17157
                     /locus_tag="IVD64_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148326.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            17172..18164
                     /locus_tag="IVD64_001062"
     CDS             17172..18164
                     /locus_tag="IVD64_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6148327.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            18176..18913
                     /locus_tag="IVD64_001063"
     CDS             18176..18913
                     /locus_tag="IVD64_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148328.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            18903..19625
                     /locus_tag="IVD64_001064"
     CDS             18903..19625
                     /locus_tag="IVD64_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6148329.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(19709..20332)
                     /locus_tag="IVD64_001065"
     CDS             complement(19709..20332)
                     /locus_tag="IVD64_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6148330.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            20667..21101
                     /locus_tag="IVD64_001066"
     CDS             20667..21101
                     /locus_tag="IVD64_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6148331.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            21124..21444
                     /locus_tag="IVD64_001067"
     CDS             21124..21444
                     /locus_tag="IVD64_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148332.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            21585..21776
                     /locus_tag="IVD64_001068"
     CDS             21585..21776
                     /locus_tag="IVD64_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148333.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(21847..22104)
                     /locus_tag="IVD64_001069"
     CDS             complement(21847..22104)
                     /locus_tag="IVD64_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP6148334.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            22262..22525
                     /locus_tag="IVD64_001070"
     CDS             22262..22525
                     /locus_tag="IVD64_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148335.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            22549..22737
                     /locus_tag="IVD64_001071"
     CDS             22549..22737
                     /locus_tag="IVD64_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148336.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(22820..23005)
                     /locus_tag="IVD64_001072"
     CDS             complement(22820..23005)
                     /locus_tag="IVD64_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148337.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            23220..23591
                     /locus_tag="IVD64_001073"
     CDS             23220..23591
                     /locus_tag="IVD64_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6148338.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(23741..23813)
                     /locus_tag="IVD64_001074"
     tRNA            complement(23741..23813)
                     /locus_tag="IVD64_001074"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23778..23780),aa:Gln,seq:ctg)
     gene            complement(23859..24287)
                     /locus_tag="IVD64_001075"
     CDS             complement(23859..24287)
                     /locus_tag="IVD64_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148339.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(24347..25105)
                     /locus_tag="IVD64_001076"
     CDS             complement(24347..25105)
                     /locus_tag="IVD64_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP6148340.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            25400..25597
                     /locus_tag="IVD64_001077"
     CDS             25400..25597
                     /locus_tag="IVD64_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148341.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            25703..26185
                     /locus_tag="IVD64_001078"
     CDS             25703..26185
                     /locus_tag="IVD64_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148342.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(26243..26839)
                     /locus_tag="IVD64_001079"
     CDS             complement(26243..26839)
                     /locus_tag="IVD64_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6148343.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            27226..28692
                     /locus_tag="IVD64_001080"
     CDS             27226..28692
                     /locus_tag="IVD64_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP6148344.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT         9676 a         4772 c         5803 g         8484 t
ORIGIN      
        1 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
       61 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
      121 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
      181 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
      241 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
      301 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
      361 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
      421 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
      481 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
      541 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
      601 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
      661 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
      721 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
      781 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
      841 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
      901 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
      961 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
     1021 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
     1081 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
     1141 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
     1201 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
     1261 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
     1321 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
     1381 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
     1441 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
     1501 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
     1561 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
     1621 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
     1681 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
     1741 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
     1801 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
     1861 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
     1921 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
     1981 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
     2041 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
     2101 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
     2161 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
     2221 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
     2281 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
     2341 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
     2401 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
     2461 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
     2521 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
     2581 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
     2641 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
     2701 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
     2761 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
     2821 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
     2881 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
     2941 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
     3001 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
     3061 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
     3121 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
     3181 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
     3241 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
     3301 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
     3361 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
     3421 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
     3481 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
     3541 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
     3601 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
     3661 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
     3721 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
     3781 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
     3841 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
     3901 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
     3961 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
     4021 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
     4081 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
     4141 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
     4201 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
     4261 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
     4321 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
     4381 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
     4441 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
     4501 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
     4561 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
     4621 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
     4681 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
     4741 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
     4801 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
     4861 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
     4921 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
     4981 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
     5041 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
     5101 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
     5161 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
     5221 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
     5281 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
     5341 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
     5401 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
     5461 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
     5521 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
     5581 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
     5641 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
     5701 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
     5761 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
     5821 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
     5881 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
     5941 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
     6001 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
     6061 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
     6121 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
     6181 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
     6241 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
     6301 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
     6361 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
     6421 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
     6481 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
     6541 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
     6601 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
     6661 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
     6721 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
     6781 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
     6841 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
     6901 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
     6961 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
     7021 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
     7081 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
     7141 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
     7201 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
     7261 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
     7321 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
     7381 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
     7441 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
     7501 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
     7561 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
     7621 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
     7681 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
     7741 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
     7801 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
     7861 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
     7921 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
     7981 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
     8041 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
     8101 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
     8161 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
     8221 tgcgccttgt tggctaaaca tcctaatata gtagcgggcg tgtgtttgat gggttcccct
     8281 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
     8341 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
     8401 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
     8461 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
     8521 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
     8581 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
     8641 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
     8701 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
     8761 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
     8821 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
     8881 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
     8941 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
     9001 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
     9061 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
     9121 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
     9181 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
     9241 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
     9301 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
     9361 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
     9421 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
     9481 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
     9541 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
     9601 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
     9661 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
     9721 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
     9781 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
     9841 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
     9901 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
     9961 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    10021 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    10081 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    10141 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    10201 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    10261 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    10321 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    10381 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    10441 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    10501 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    10561 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    10621 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    10681 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    10741 tcgcagatcg tttgcaaaaa tagtttattt ttttataaag aagattttac tgaataatat
    10801 cattttagtt tacataatga tatttctgta aattaaataa agtagtgaaa aataagaatc
    10861 agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt ttctgtagtc tgttttgctt
    10921 ccaattgggg caaaacagat ccaatttcca gatacttcac tttattcaat gtataagata
    10981 gaatagaaca gctaagtaat gtatacagaa aatgtgtgag atccaaataa agcaaggaga
    11041 caactaaaat accaaaagta atcaagaaaa gacttttgcc aatcttaata tgaaaatatt
    11101 tttcaatggc ttgataaatg atatctgttc cgcctgtgct tccgccgaat cgaaagacga
    11161 gaccgcaacc aatgccagaa agaatacctg tcatcaaact attgatcagt agactatcta
    11221 cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa gaaaactagc gcaaatgttc
    11281 cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa caatgagata agcaataaag
    11341 gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc aaaaagatta tgcaaaagca
    11401 aagcaatacc agtaacccca ccatctgcca gcttagatgg aatattgatt cctgtcacag
    11461 ctaatccgta gattcctgcg cctaaaatga ttccgcacaa ttctgtaaat aaatttaatt
    11521 ttttcatttc tagcctcttt tcttttgaaa attttcagca acttctttat tatactgact
    11581 tatactctta cttcaagagg aaaatgaaaa aaaattattt ttggctgttt ctttttattt
    11641 atcaaataag gagtggaaaa atgcgagcag cagacttatt gacaattctt acaaaatcac
    11701 ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga acaaaaactg gtgcctgtga
    11761 caatggtcca gataaataaa gcaaatcaat ttatcttttt atttgaacac ggcaaagcac
    11821 cacttgctat gaaagacatc tttctttttt tgatgaagaa ccgtgagaaa gctgtttatt
    11881 atcagtcaaa agaagaactc attcctcttt atggagtgaa agagatagaa aataaagtgg
    11941 tgatataagt aaaaataaga aaacaagacg tttatgatgt caaaacaacc attttttaca
    12001 tttttggcaa aaaagtgtca tgatacagta taatattcgt tgaacaggcc aataaaggtg
    12061 ttctccccca cgagatttct actttattgg ctgtttcttt ttattaaaat aaaagttgtg
    12121 tgtgtaaaaa agatatagct gattccaagt gacaatttcg tatagatttt tatggtattc
    12181 gtagtgaaaa aacgagaacc aaaaagttga tccgactttg ttgcttggat tctttttttg
    12241 tcagcatatt tagaggtatt tttttctttt ttttgttcgt gctgtaaggt taaggcaaga
    12301 caagttagtt gggaaaaagg aaagaaatgt ttatacttaa agaataacag aaaggaaggt
    12361 cgcatgaata attttataca aacattaagt gaaagaaaag gcgagttgct ggaagctgca
    12421 tttcaacatc tgtccatttc tctcatttct ttgctgattg cagcactgat tgctattcct
    12481 ttagcgattt gggcagccaa tcataaaaaa atggcagaag tcttattgca gatcacgggt
    12541 gtgctccaaa cgattccttc acttgcctta ttaggtttac ttatcccatt tgtagggatc
    12601 ggaactgttc cagcattgat tgcacttgtt atttacgctt tgcttccgat tttccaaaac
    12661 acgtatattg gtctagctga aattgacccg tctattgaag aagcagcggt agcatttggt
    12721 atgtccagaa tgagacgctt gctgaaagta gagcttccca tcgcattacc tgtcattatc
    12781 tcaggtatcc gaactgcctt agttttaatc atcggtacgg ctactttggc agcattgatc
    12841 ggagcaggtg gattagggac atttattttg ttaggtattg accgaaatac acctgttttg
    12901 acattgattg gtgcgatcag ttctgccttg cttgcaatta tattcagcgg actgattcgc
    12961 tggttgcagc ataagaaacc tcgatattcc atcattactt tagcagcagt ggtgtgttta
    13021 tttggcggcg tttctttata tcagtctgcc ttgtttaaaa atgaaaccat cacgattgct
    13081 ggaaaattag gagcggaacc tgaaatcctg atccaaatgt ataaagaatt gattgaagac
    13141 aacacaaaaa cagaagttga attaaaaccg aattttggca agacgagttt tttgttcaat
    13201 gcactggatc aaggtcaaat cgatatctat cctgagttca caggtactgt cctagaaagc
    13261 ttggtgaagg tacctgaagc aatgaaaagt gctgatttga caaaagaaaa aacatatgac
    13321 gaagcagaag aattattgaa taagcaattc gatatgacat tattaaaacc aatggcttac
    13381 caaaatacgt atgccttggc agtcaagaga tcttttgcga acgaacatca acttaaaaag
    13441 atttccgatt tggcagctat ctctgatcag atcaaagcgg gattcacact ggaatttatt
    13501 gatcgttcag atggatataa aggaatccaa gaagcatatg gcttgaattt ttcaagtgta
    13561 caaagcatgg agccaagttt gcgttaccaa gcaatcaata atggagatgt caatattgtg
    13621 gatgcctatt ccacagatag ccagctcaag gagtacgatc tagtgactct agaagatgat
    13681 cgagggttat tcccagctta tcaaggcgca ccgctgatga aaaaagattt tgctaaagaa
    13741 catcctgaaa tcgttaaagc actgaataaa ttaagcggaa aaataacaga ggatcaaatg
    13801 attgatatga attaccaagt caatgtcgaa aagaaaaagc cgacagaagt agcacgaatc
    13861 ttcctgaaaa atgaaggatt agtaaaggag gaagatcagt gactaaatta atcgagttca
    13921 aacatgtgca aaaaaagtat gatggaaagt atgtcatcga tgacctaaat ctggcaatca
    13981 aaaaaggaga aatctttgtt ttggttggtc cttccggtag tggaaaaaca actactttga
    14041 aaatgatcaa tggattatct aaaccatcag caggagacat ctacttcaaa ggaaaatcgt
    14101 taaatgaata taatctgcag aaaatgcggt ggaatatggg atatgtgctg cagcaaatcg
    14161 ctttgttccc gaccatgaca gtcaagcaaa atattgaagt catccctgaa atgttaggtt
    14221 gggaaaagca gaaaagagca gaccgtgtag atgaacttct tcaaaaagtt ggcttatctc
    14281 ctgatattta tcgagatcgt atgcctagag agttgtctgg aggagaacag caacggatcg
    14341 ggatcatccg agctattgct gcaagcccag atgtgatttt gatggacgag ccatttagcg
    14401 cattagatcc gatttcaaga aactcattac aagaattggt tttgtcctta cacgaagaat
    14461 tgggcacgac catcgttttc gttacgcaca atatggaaga agcaatcaag ctaggtgatc
    14521 gtattgcatt tatgaaggat ggagaaatta tccaatgtga cacaccagag cagttattga
    14581 tgaatcctaa aaacgactac gttcggcatt tctttgatga accgaaacag acgaaagagt
    14641 ggcgagtaga agatttagtc gtcaatggat atttcttaaa tgaaatacca gaaaatgctc
    14701 gtcaacaagt ttgctttgat acgcctatga aagaagtata ttcgcttctt tcggtgtatc
    14761 caagcattgt gatagtagaa aagcaaagaa gtattgggtc gctgacaagt aaagaaatct
    14821 ttgctttttt aagtcgagag gaggaaggaa atgaaaacat atgatgcaat catcatcggt
    14881 tcaggtgtga gcggcttaag tgctgcttat ggattgaaag aagcaggtaa aacagtacta
    14941 gtggtcgaag aagatctatg gggcgggact tgtcctaacc gtggatgtga tcctaaaaaa
    15001 gtcttgttaa gtgcagtaga ggcacgtaat cgggtaaaac aattgagtgg aaaaggtttt
    15061 aacgagatcc caactgccaa ctgggaagaa cttcaaaaat tcaaacgaac atttacggat
    15121 ccagtcccag aaagccgaaa aaaacaatta gcagaagctg aaatcgatca tctttcagga
    15181 accgctcgtt ttttggatga ttcatctatt gaagtcaacg aagaagtctt ccatgcggat
    15241 tacttagttt tggcaaccgg gcaaagacct acgatcttac cagtggaggg aaaggaatac
    15301 ttgaaaacta gtgcagactt tttatcattg cctgtactgc ctaaagaaat catttttatc
    15361 ggtggtggtt atattgcttt tgagctagct acgattgcca atgctgcagg gagcaaagta
    15421 accattgtcc atcataatca acggccatta aaagaatttg aggcgtcttt ggtagaggaa
    15481 gccgtccatc aaatggaagc atctggcatt cagtttgctt ttggtgttga aacccaaaag
    15541 atcatttcag aaggtactcg ctatcgttta gtcggaaaag agacggaatt agtagcagat
    15601 atgatttttt gtgcgactgg gagacagccg aatacagaat ctttagcact tgagcaagcg
    15661 aatatcgtct ttgataaaca cggaattgca gtcaatgatt atctgcaaac gagcaatcca
    15721 aaaatttttg cgtgtggaga tattgtttca aggaaaaccc caaaattgac accagtcgcc
    15781 acttttgaag gaaattatgt agctaagcgg ataacggatg caacaagcga acctatcaaa
    15841 tatccgatta tacctacgat cgtctacgcc agtcctaaac ttgctgaagt aggagttaca
    15901 aaaagtcacg cctcttcgtc agatcaagtg gtagagatgg atcttaccag ttggttcacc
    15961 tatcatcgtg tcaatgaacc agttgctaag gctgaattga cctttgacca gcaaaattat
    16021 ttgattggtg ctgcagtgat tagcgagcaa gcagacgaat tgatcgatga tttgacactc
    16081 gtcatcaatc aaaaattgac taaaaaagag ttggatagct atatcatggg gtatccgaca
    16141 ttggcaagtg atctctccta tttattgaaa taatcagcat gataactaaa gcgcttgttg
    16201 ctttttcctt cttgtgctac actttaaaaa atgaatacct gtttctgggg atgtcgtaga
    16261 cgacatgaga gtaagttgtg aacttagacc cattgaacct gtcagttaat actggtgaag
    16321 ggagaaacat agcagacgct tgaagcgggt tactatgtcg tgcccatttc aagcgttttt
    16381 tgttattcat attagaagaa aggaggtaac aaaatgaaga aaaatcttta tccagcagtc
    16441 agtatggcat tcattttaat tatttggcag attgttgtca tagtttggaa aatacctgcc
    16501 tttattttac ccggacctgc tgctgtattt tctgcattag cggcagatgc acaagtatta
    16561 ctttcccatt ctgtcacgac attaaatgaa gcagtgcttg gtttggtaat agctagcgtt
    16621 ctttctttta ttactgcctt ggcaatggat tattggaagt ttttagaaat gtctttttat
    16681 ccgttactag tgatttcaca aatgttgcca atcatggtgt tagggccatt acttacccta
    16741 tggtttggtt tcggtatgct tccaaaagtg atccttgttg tcttgataag ctattttccg
    16801 atcgtagttt ctttttcgga tacattgagg aaagtatcaa aagagcaaat catctttttg
    16861 aaaacgatgg gggcagatac agggaagatt tatcgaatat ataaaatacc agcgggttta
    16921 tccggcttct tttccggttt aaaagtggct gctacttatt gtgtcagcgg agcagtcgtt
    16981 ggagaatggc taagcgctca agcaggctta gggtattaca tgatacgcgt aaaaaacagc
    17041 taccagatcg ataaagtttt tgctgcgatc atttgtgtca ttctattgag tttattgctg
    17101 aatggcgctt gttcattatt gaaaaaaggg tattatcgta tgctttatat tttttaagag
    17161 aaggggatca aatgaaaaaa ttatggcttt tattaccgct actgttgctt ttatccgctt
    17221 gtggcactgc aaaagaaaca agtagcaaac aagaaataaa ggatttgaaa aaagtgacac
    17281 ttgttttgga ttatgtaccg aatactaatc atactggcat ctacctagca aaagaaaaag
    17341 gctattataa agaggctggt ttaaatgtcc aaatcatcga accaggagat aattcgacaa
    17401 gtattggtct agtaggtgcg gacaaagcac aatttggtgt aagttatcaa gaagatgtca
    17461 cttatgctca cgcagatgga cagaatatac cagtcaaagc aattgctaca gtgatcaaac
    17521 ataatacttc tggattcgcc accctatcag acagtaacat ccattcacca aaagattttg
    17581 aaggaaaaac atatgcaggt tggcaaagtc caagtgaaga agctgtctta aaagctgtga
    17641 tggaaaaaga cggcggggac ttttcaaaac tgacaatggt aggaagcaat ggagaagggc
    17701 cagaaagctt aggaaaatcc tcagatatcc aatggtactt tgaaggttgg gatatgatca
    17761 aagcaaaaga agcgggtatt gaactaaact atatcccatt gaaagaatta gatgaacggt
    17821 tagattacta tactccggta attattacga atgatcaact gatcaaaagt gatccagaat
    17881 tggtacagtc ttttatggat gccacgaaaa aaggctatca ggaagctatt aaggatccaa
    17941 atgacagtgc taaactgttg caaaaatatg caaaagaaaa tgatcggacg tttttagaag
    18001 aatcacaagc attcttgtca aaaaattata cggatgatcc tgagaattgg ggattgatgg
    18061 aagaaaaagt ttggcgcaat tatacagctt ttatgcagga aaacggattg atcaagcaag
    18121 atgtaccttc gcaagaacta ttcactaatc agtttataaa ataggagtga tagatatgtc
    18181 aatccaaatc agccacttga atgtagtcat tgaaaacctg aaaatattag aagacatcca
    18241 tttcactatc cctaaagata cctttacatc gattgtcgcg ccaagcggag ccggtaagtc
    18301 aacattgctg aaaactttaa caggagtcca gcctatgact tctggaacga tcaaagtaga
    18361 cgatcaaaaa gtaactggtc tccatacagc atttagttat atgccgcaag aagatatgtt
    18421 acttccttgg ctgagcgtct atcaaaacgt tacgttatat caaaaaatca atcatctaac
    18481 aatcaatgaa gaacaagtaa gggattatct ggatatcttt gggttaaacg gctatgaaca
    18541 ttttttgccg gaacaattat ctggcgggat gaagcagcga acggccttgt tacgaacaat
    18601 catgaatcct tcttcttact tgcttcttga tgaaccattt ggagcgttag atgctatgac
    18661 cagagggcag atgcaagact ggatgttgaa gctgcctaaa aaagcaaagc ggactacttt
    18721 acttgtgacg catgatatcg aagaagcaat ttatttatca gacaggattc ttgtgctttc
    18781 cgcccgtccg gcacatgtga ttgctgagat tcaagtgcct gaaaaacgac ggtcacgtga
    18841 atggctactg cagcaatcag agttgaagca aacgatttat cagttattgg caggtgaatt
    18901 agatgttaag tgatgctcat tgtcatttag atgggagtca gagtttagca ctccttcagc
    18961 aggaacacac gattttgacg atcatcaatt gtgattcgcc agaagaatgg aaggaaaaca
    19021 gacagcttgc agcaagtaag actcaagcct taagttacgg tatccatcct tggaaagcag
    19081 atagctatac gtttgaacaa gttgagcctt ttttgaaaaa agcaaggatc atcggtgaaa
    19141 tagggttaga taatatttgg acgaatgtac cgatgactac acaaaaaaaa gtatttgagc
    19201 gacagcttgc ttttgcagcc ataaatgaga aacctgttgt actgcacaca aaaggatgcg
    19261 aaaaagagat cttaaattat atccaagctt accctaatca ctatttgatc cattggtatt
    19321 cttctttgga ctatcaacaa gactatattg atttaggctg ttatttcagc attggtcttg
    19381 atttgaaaaa gaatccagca gtctggcaac tagctaaagc agtcccaatc gaccacttac
    19441 ttattgaaac ggatggacta catgcagcag agtgggcttt aggggggatt ttttcagaaa
    19501 aagattatgt ctcactgctt gaagaacagt taaagacaat cgctgagatc aaagagctat
    19561 cagttgaaga agttaaaaaa aatacttatc aaaacttgga acgtttcatt agattaggta
    19621 aataaaaaag agatgtgaca caactagtgt cgcacctctt tttggctttc tttgtataaa
    19681 agtaaactgt ttgattaata aatgaatctt agtaccaacc gtttgcttcc cagaatgctt
    19741 tcgccgcttc ccaagaacca taacgtgaag taacatattg ttctgctact ctttcttggt
    19801 ttgcagcaga gtagtcacca tttaagtaag aagaatctaa ttggtaacgt ccgatgtaac
    19861 gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg tgcgatccat tcttttgcag
    19921 agcttgtgct tgctggagta gcagcagttt ctgttacagc aggagcaggt tgtgtttcag
    19981 caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt
    20041 ggactggtgc ttcttgaacg gctgcttgag gttcagcagc tgcttgtgca gagccttctg
    20101 ttgggattgt taattgttgt cctgagtaaa tcaggttaat atcagaaatt gaatttgatt
    20161 ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc gttgatagag
    20221 tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc atgaagaatg
    20281 cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc atgaaaaatc
    20341 tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt taacacgtga
    20401 aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt ttttgtaaca
    20461 gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca gaaataatgg
    20521 gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa gaaattgctt
    20581 ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt gagatgttac
    20641 aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc atggtagcag
    20701 ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta gcaaaggaca
    20761 atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc catagtgcct
    20821 atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc atgctgaaaa
    20881 aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt gaaactggag
    20941 atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat ttgatcgtta
    21001 tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc gcttcttatg
    21061 tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag ggaggaatag
    21121 aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat tttagcaatt
    21181 attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa gtggaaagga
    21241 aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg attccctgtc
    21301 acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat ggaattatac
    21361 agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt aacagaagag
    21421 cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga aaactgatta
    21481 gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa tgcgtataac
    21541 ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa gcaggaaaag
    21601 tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg aaaagcctga
    21661 tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa tttatagaat
    21721 taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca gaatagaaaa
    21781 aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt taaacatgcg
    21841 agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa aaattactga
    21901 aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat gcctaaaaga
    21961 aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg aaaacttctt
    22021 ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag gaatataaaa
    22081 aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc atgatacaaa
    22141 ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta aaatctgtat
    22201 cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga gtgggatttt
    22261 catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc gggtgattta
    22321 ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa atggtatttt
    22381 agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata gtatcccagt
    22441 agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca aaaaagcata
    22501 tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat gaatccatat
    22561 gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt aaaaagagaa
    22621 gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata tgtggtggga
    22681 aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt attttagtat
    22741 gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc ctttgatttt
    22801 acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg acactgtcga
    22861 tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat tgttctttat
    22921 gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt tccttgactt
    22981 tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac atgatttttt
    23041 acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa ataacgatca
    23101 tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc tccaacttgg
    23161 gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga ggtagaaaga
    23221 tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg ctatttggac
    23281 tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat cagctaaatc
    23341 cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa gtaaatgaat
    23401 atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa cgaacggttg
    23461 aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc aataaaggag
    23521 cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa gcattagcaa
    23581 ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt cataatttaa
    23641 ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca aaaagattgt
    23701 tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc agggttcgaa
    23761 cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc ccatttattc
    23821 aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg atcggatagt
    23881 tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc aggaagggca
    23941 atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac aggttcattc
    24001 ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata ctctgttcga
    24061 gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc gtaataaaca
    24121 agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct gcgacgctcg
    24181 ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg gacaaaaata
    24241 atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat ctacgctccc
    24301 ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag cttttctcca
    24361 aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg atcggtactg
    24421 ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca tatccagtga
    24481 ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg gtccaagtca
    24541 taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag acaggatttc
    24601 ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga aaagtgtgat
    24661 aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag acgaccatag
    24721 cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca tgagtcccat
    24781 ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta gttgtttcat
    24841 aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt tctaaagaag
    24901 gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc tcttttgtct
    24961 ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct ggagaatgga
    25021 tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct agaaacaata
    25081 gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt atctttccta
    25141 ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag gaaataatta
    25201 gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca acagctgttt
    25261 taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt taactgtaaa
    25321 taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag aaaagttcca
    25381 gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac aatatgcatg
    25441 caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa tgacaaactg
    25501 cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag atgaaaaagc
    25561 aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg acgtgcggcg
    25621 tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat tttctggaga
    25681 agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt ttttattcgt
    25741 tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc ggactgtgcg
    25801 aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt ggtatacact
    25861 catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa tgatttttat
    25921 tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga atagctttga
    25981 caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc caaaaggttc
    26041 taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc tgaaattttc
    26101 tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg aggcagagat
    26161 tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt gtcacaacct
    26221 ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca tcgttcatgt
    26281 attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa cgtagtttcc
    26341 agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg ttgtaatgtt
    26401 gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct gttgcgattc
    26461 cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat tgttcttgta
    26521 gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca tttttgaaac
    26581 cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg cgaagtgctt
    26641 cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact gcgcgtgttg
    26701 attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa gttgttaatt
    26761 cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg tttttcaaag
    26821 tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc atttttttaa
    26881 ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata tgctcacaaa
    26941 aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa ggattttctt
    27001 agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa aacagctgat
    27061 atataattat taaatctcta tcttcttttt tttatgatat tgccattaaa aattacttgc
    27121 ttttatagaa aattagttta tatttcttaa ataagttttt tagttaaatt tttcggtaaa
    27181 gatgtataat attttctgaa aatttaaaga attggggtat agattatgga aaaacagcaa
    27241 gacaagggct tcttcgggca gccgaaggga ttgtcaactt tgttcttcac tgagatgtgg
    27301 gaacgattta gttattacgg tatgcgtgca ttacttatct attatatgta cgatacagtt
    27361 gccaacgggg gacttggatt accacgagca acagcattag ctatcatgtc gatctacgga
    27421 tctttgatct acatgtctag tatcgtcggc ggatgggtat ctgaccgagt gttaggagca
    27481 aaacgcactg tattcttcgg tggagttttc attatgcttg gccacatcgt cttggcttta
    27541 ccatttggtg taagtacatt gtttgtttcg atggcattga ttattatcgg aaccgccttt
    27601 ttgaaaccaa atgtttctgg catggtcgga catctttatt caaagactga cttgcgaaga
    27661 gatgcaggat tttcgatttt ttacatgggt gtcaacttag gggcattatt agcaccacta
    27721 atcgttggta caatcggtca agaatataac tatcatttag ggttctcttt agctgctatt
    27781 gggatgttct ttggattatt acaatacatc atccaaggac gcaagacatt agacggagtg
    27841 ggtacaacac cgccgaatcc tttgacaact gaagaacgta aaggattcat ccgtaacttg
    27901 atcatcgcac ttgttgttat cgctgcagta ttcggcgggg cacaactaac tggtcatctg
    27961 acaattgact tttttgtcaa tacaatcagt gtattgggga ttatcttacc gttgtattac
    28021 tttatcaaaa tgctgacttc aaaagatgta actgctgaag aaaaaccaag agtatgggct
    28081 tatatcccat tattccttgc agcaatcatt ttctggtcat tacaagaaca aggttcatct
    28141 atcttagctt tatttgctag tgaacgtaca caatctacgc tatttggtgt aacgatccaa
    28201 gcaagctggt atcaatcatt gaatccaatt ttcatcttta ttctgacacc agtattcgta
    28261 acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag tagtgaaatt ctctcttgga
    28321 cttttatttg caggattatc gtacttgcta ttgatgttgc cgggtatgct ttatggtaca
    28381 agtggaaaag taagtccatt gtggttgttg ggaagtttct tcatcgtggt tattgctgaa
    28441 ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat tagcaccacg agcatttgaa
    28501 tcacaaacaa tcgctatttg gttcttagct gatgcttctt cacaagcaat caatgcacaa
    28561 attgcacgct tctacacacc gggaacagaa tctgcttact tcggtattat cggtgcagta
    28621 gcaatcgtcg gtggtatctt gctatttact gtgaaagaac caatcaaaaa actaatgggc
    28681 tctattcatt aatcaatgaa cataaaaaca ggaattgctt atttgcagca gttcc
//
LOCUS       DACBZN010000024        28684 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.024, whole genome shotgun sequence.
ACCESSION   DACBZN010000024 DACBZN010000000
VERSION     DACBZN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.024"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(23..1189)
                     /locus_tag="IVD64_001081"
     CDS             complement(23..1189)
                     /locus_tag="IVD64_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6148345.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(1201..1554)
                     /locus_tag="IVD64_001082"
     CDS             complement(1201..1554)
                     /locus_tag="IVD64_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6148346.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1682..2704
                     /locus_tag="IVD64_001083"
     CDS             1682..2704
                     /locus_tag="IVD64_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP6148347.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(3143..3577)
                     /locus_tag="IVD64_001084"
     CDS             complement(3143..3577)
                     /locus_tag="IVD64_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148348.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(3687..5546)
                     /locus_tag="IVD64_001085"
     CDS             complement(3687..5546)
                     /locus_tag="IVD64_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6148349.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(5664..6823)
                     /locus_tag="IVD64_001086"
                     /pseudo
     CDS             complement(5664..6823)
                     /locus_tag="IVD64_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(7130..8554)
                     /gene="araA"
                     /locus_tag="IVD64_001087"
     CDS             complement(7130..8554)
                     /gene="araA"
                     /locus_tag="IVD64_001087"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP6148350.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(8570..9268)
                     /locus_tag="IVD64_001088"
     CDS             complement(8570..9268)
                     /locus_tag="IVD64_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6148351.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(9225..10886)
                     /locus_tag="IVD64_001089"
     CDS             complement(9225..10886)
                     /locus_tag="IVD64_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP6148352.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            11104..12504
                     /locus_tag="IVD64_001090"
     CDS             11104..12504
                     /locus_tag="IVD64_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP6148353.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(12756..13835)
                     /locus_tag="IVD64_001091"
     CDS             complement(12756..13835)
                     /locus_tag="IVD64_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6148354.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(13921..14880)
                     /locus_tag="IVD64_001092"
     CDS             complement(13921..14880)
                     /locus_tag="IVD64_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP6148355.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(14892..16415)
                     /locus_tag="IVD64_001093"
     CDS             complement(14892..16415)
                     /locus_tag="IVD64_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6148356.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(16425..16988)
                     /locus_tag="IVD64_001094"
     CDS             complement(16425..16988)
                     /locus_tag="IVD64_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6148357.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(16994..17857)
                     /locus_tag="IVD64_001095"
     CDS             complement(16994..17857)
                     /locus_tag="IVD64_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6148358.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(17871..18767)
                     /locus_tag="IVD64_001096"
     CDS             complement(17871..18767)
                     /locus_tag="IVD64_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148359.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(18942..20213)
                     /locus_tag="IVD64_001097"
     CDS             complement(18942..20213)
                     /locus_tag="IVD64_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6148360.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(20203..21720)
                     /locus_tag="IVD64_001098"
     CDS             complement(20203..21720)
                     /locus_tag="IVD64_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6148361.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            21918..22808
                     /locus_tag="IVD64_001099"
     CDS             21918..22808
                     /locus_tag="IVD64_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP6148362.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(22881..23705)
                     /gene="nadE"
                     /locus_tag="IVD64_001100"
     CDS             complement(22881..23705)
                     /gene="nadE"
                     /locus_tag="IVD64_001100"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP6148363.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(23717..25189)
                     /locus_tag="IVD64_001101"
     CDS             complement(23717..25189)
                     /locus_tag="IVD64_001101"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP6148364.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(25826..26188)
                     /locus_tag="IVD64_001102"
     CDS             complement(25826..26188)
                     /locus_tag="IVD64_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6148365.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            26411..27163
                     /locus_tag="IVD64_001103"
     CDS             26411..27163
                     /locus_tag="IVD64_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6148366.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(27303..28226)
                     /locus_tag="IVD64_001104"
     CDS             complement(27303..28226)
                     /locus_tag="IVD64_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6148367.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(28241..28513)
                     /locus_tag="IVD64_001105"
     CDS             complement(28241..28513)
                     /locus_tag="IVD64_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148368.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         8478 a         5987 c         4883 g         9336 t
ORIGIN      
        1 ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa
       61 gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat
      121 aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa
      181 ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat ggagagatca
      241 agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg
      301 gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag
      361 ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga
      421 ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta
      481 tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc
      541 gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa
      601 ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac
      661 ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca
      721 tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca
      781 aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc
      841 tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa
      901 caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt
      961 aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta
     1021 cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga
     1081 cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc
     1141 agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg
     1201 ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata
     1261 gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc
     1321 tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc
     1381 atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg
     1441 aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg
     1501 ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact
     1561 ctctccttta attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa
     1621 taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca
     1681 tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac
     1741 gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa
     1801 tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga
     1861 tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga
     1921 gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc
     1981 gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc
     2041 agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca
     2101 tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga
     2161 tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac
     2221 tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta
     2281 tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc
     2341 ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg
     2401 ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa
     2461 ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat
     2521 ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt
     2581 gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt
     2641 actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg
     2701 ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga
     2761 actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata
     2821 tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa
     2881 ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct
     2941 gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaaaggagta
     3001 tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc attatcataa
     3061 aggattttga tcactctatc ataaaggtaa tattacattt atattagata actctagttt
     3121 ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg
     3181 cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt
     3241 catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg
     3301 aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag
     3361 tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga
     3421 tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga
     3481 tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat
     3541 cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat
     3601 tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt
     3661 aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta
     3721 tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt
     3781 tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt
     3841 tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc
     3901 tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga
     3961 gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat
     4021 gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc
     4081 caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt
     4141 gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt cccacttcca
     4201 tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg catatgtgtc
     4261 tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag tgtcattact
     4321 tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc cgtataggtc
     4381 atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc ctctctttta
     4441 tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc gtttaacgct
     4501 gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg ggtggtgagt
     4561 tgcgcattaa ataacgccat agaaaaacca gtcaatgctt caaattcgat atttgctgtt
     4621 ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc tacttcataa
     4681 taatctgcca atggctcacc agatacttct acgccgttta atcgactagg atctgagaaa
     4741 ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt atttttttca
     4801 tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga atatccttca
     4861 ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa accagtgtta
     4921 tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata tgcttttcgc
     4981 aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat tgccacaagc
     5041 agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg aaacatgtac
     5101 cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg
     5161 ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc tggataaatc
     5221 ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc gatggctacg
     5281 acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa aataagaata
     5341 atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa agcaaatttc
     5401 aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt actaaaaaca
     5461 acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt ctgtttggtc
     5521 gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca ataaaagaat
     5581 gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa
     5641 tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa gcggaccttt
     5701 cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc aaaatcagta
     5761 ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga tatatctgcg
     5821 atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt ggttttcttt
     5881 tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga tggataattg
     5941 accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac tgccaaacgt
     6001 ccgctgtctg ccaaagcttc ctgagtcgat cccctagatg aggcaacata agtatacggt
     6061 cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt
     6121 ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta
     6181 cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg
     6241 tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt
     6301 gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc
     6361 tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt
     6421 tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat
     6481 taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga
     6541 gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga
     6601 cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc
     6661 ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa
     6721 ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta
     6781 tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact
     6841 aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc
     6901 aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact
     6961 gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt
     7021 tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt
     7081 caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa
     7141 ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta
     7201 gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga
     7261 aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg
     7321 gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg
     7381 atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat
     7441 ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta
     7501 cttccatcat atgggattcc aagattgctt cttggccact agctagctcg taagtataat
     7561 cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg
     7621 cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag
     7681 ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat
     7741 agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat
     7801 gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact
     7861 cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc
     7921 cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt
     7981 ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt
     8041 cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt
     8101 tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt
     8161 cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag
     8221 gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc
     8281 cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt
     8341 cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt
     8401 cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt
     8461 gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa
     8521 caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt tatttgtttt
     8581 gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag
     8641 gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat
     8701 ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt
     8761 gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt
     8821 ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa
     8881 atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa
     8941 catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc
     9001 cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga
     9061 gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct
     9121 cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc
     9181 tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt
     9241 atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga
     9301 atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct
     9361 gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct
     9421 gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg
     9481 attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca
     9541 gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa
     9601 cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa
     9661 gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt
     9721 acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa
     9781 gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat
     9841 aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc
     9901 caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa
     9961 ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc
    10021 atagcgaagg cggaagtacc agccgaaatg ttccctgtcc ctttggctac actgttcgta
    10081 gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa
    10141 tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct
    10201 gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt
    10261 aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga
    10321 aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca
    10381 taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata
    10441 gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt
    10501 aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac
    10561 tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg
    10621 attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg
    10681 acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg
    10741 ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct
    10801 ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac
    10861 atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga
    10921 tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc
    10981 aagcagttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa
    11041 aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata
    11101 cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc
    11161 ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat
    11221 gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta
    11281 ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa
    11341 atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct
    11401 cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct
    11461 gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga
    11521 ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat
    11581 tatttgctaa aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct
    11641 gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt
    11701 atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa
    11761 gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga
    11821 acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt
    11881 gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc
    11941 gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga
    12001 atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga
    12061 actttattga aaataggcgg tagcgtgatg ggactttctt tcatcctacc agctgtttta
    12121 ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat
    12181 gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcgtagg cgaaatattc
    12241 ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct
    12301 ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc
    12361 ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa
    12421 actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa
    12481 cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata
    12541 agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    12601 tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt
    12661 gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac
    12721 cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt
    12781 tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc
    12841 gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt
    12901 gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg
    12961 tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga
    13021 tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt
    13081 tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc
    13141 acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg
    13201 tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc
    13261 ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca
    13321 gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt
    13381 gggttatact gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc
    13441 attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat
    13501 gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa
    13561 tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct
    13621 ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca
    13681 tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc
    13741 agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt
    13801 tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac
    13861 gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc
    13921 tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa
    13981 gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc
    14041 tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt
    14101 gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt
    14161 atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc
    14221 atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag
    14281 gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga
    14341 aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt
    14401 agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga
    14461 agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc
    14521 tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg
    14581 atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc
    14641 attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc
    14701 ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa
    14761 ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt
    14821 atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat
    14881 aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct
    14941 acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct
    15001 ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct
    15061 tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg
    15121 atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca
    15181 ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct
    15241 ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct
    15301 ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt
    15361 acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc
    15421 atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc
    15481 ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa
    15541 tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa
    15601 gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct
    15661 tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct
    15721 tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca
    15781 actaagattg tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct
    15841 aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc
    15901 cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca
    15961 ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt
    16021 ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta
    16081 aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt
    16141 tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt
    16201 cctccaggat agcgaatgac acctaattct aattccttta cataagagag gacatcttgt
    16261 ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca
    16321 cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa
    16381 tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct
    16441 atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa aataaaagga
    16501 gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc
    16561 aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct
    16621 ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact
    16681 acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa
    16741 taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct
    16801 tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa
    16861 aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga
    16921 atacctaata agatgtaatc agaaaccaca atcaactgtc cataaagttt agtctcaact
    16981 attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct
    17041 gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag
    17101 gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca
    17161 tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg
    17221 tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa
    17281 tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa
    17341 taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac
    17401 ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga
    17461 cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga
    17521 aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg
    17581 tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa
    17641 ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa
    17701 tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca
    17761 acaatataac tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac
    17821 tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt
    17881 tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata
    17941 aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga
    18001 ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca
    18061 tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag
    18121 gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc
    18181 atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc
    18241 atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt
    18301 ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc
    18361 cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata
    18421 actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct
    18481 actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa
    18541 gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa
    18601 cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt
    18661 cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa
    18721 tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca
    18781 tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa
    18841 caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata
    18901 tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga
    18961 ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt
    19021 tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa
    19081 tatggctctg catagtccat ttgtttcgat aattcagata caataggatc attttttact
    19141 tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt
    19201 ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta
    19261 ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct
    19321 gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg
    19381 ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc
    19441 atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc
    19501 gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc
    19561 actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg
    19621 aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat
    19681 tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta
    19741 ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact
    19801 tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag
    19861 aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt
    19921 aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa
    19981 ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat
    20041 tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc
    20101 aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa
    20161 atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt
    20221 tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca
    20281 attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact
    20341 tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc
    20401 ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac
    20461 tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc
    20521 ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg
    20581 tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt
    20641 tgtttctgtc ctgattggtg caataacatt taccaattga gcaagacaag caatctttac
    20701 tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa
    20761 attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg
    20821 ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact
    20881 atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat
    20941 gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg
    21001 cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc
    21061 aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac
    21121 gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg
    21181 tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg
    21241 gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca
    21301 atattcgact aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt
    21361 gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt
    21421 ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact
    21481 gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt
    21541 caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg
    21601 atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta
    21661 caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat
    21721 aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat
    21781 ataaatgtta aacttactta ttttttatcg attttaatta accaaaatat taataatgat
    21841 taaaacaaat aatttaatat aacttttatt aacaaatatg ttaatatata agaaaaacct
    21901 tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag
    21961 cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg
    22021 tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa
    22081 agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc
    22141 ttcgtgtcga taaaatcgat atcagttttc cagaaaaaat attcaatgct tttgatacga
    22201 aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt
    22261 tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc
    22321 gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta
    22381 attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg
    22441 aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg
    22501 agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg
    22561 attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac
    22621 acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag
    22681 agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct
    22741 gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact
    22801 acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga
    22861 cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt
    22921 cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact
    22981 tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct
    23041 gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc
    23101 ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc
    23161 aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca
    23221 tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata
    23281 cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt
    23341 aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc
    23401 tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga
    23461 cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt
    23521 agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa
    23581 gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt
    23641 tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg
    23701 ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt
    23761 ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg
    23821 gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt
    23881 tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta
    23941 aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga
    24001 acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt
    24061 cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta
    24121 ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga
    24181 tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg
    24241 tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt
    24301 catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt
    24361 cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt
    24421 tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg
    24481 tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg
    24541 cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta
    24601 tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg
    24661 ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata
    24721 gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga
    24781 aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca
    24841 cttggattaa tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg
    24901 ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac
    24961 gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt
    25021 ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa
    25081 aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat
    25141 atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact
    25201 cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa
    25261 tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc
    25321 catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt
    25381 ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa
    25441 ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata
    25501 accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc
    25561 cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg
    25621 gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac
    25681 acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg
    25741 cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta
    25801 ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca
    25861 ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca
    25921 ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt
    25981 cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat
    26041 atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag
    26101 ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac
    26161 tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga
    26221 tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc
    26281 tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt tcgtgagaaa
    26341 aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg
    26401 aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag
    26461 aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg
    26521 ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac
    26581 aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca
    26641 gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct
    26701 cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa
    26761 agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca
    26821 agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa
    26881 tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct
    26941 cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca
    27001 taagaagtgg ttctcttaca gctggcctga atatcagcca aaagcgcata aaggactagg
    27061 aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg
    27121 tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa
    27181 caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt
    27241 atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat
    27301 attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac
    27361 aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga
    27421 agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag
    27481 ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc
    27541 tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca
    27601 agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta
    27661 taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc
    27721 aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt
    27781 tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca
    27841 tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct
    27901 tcaagatacc ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa
    27961 tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg
    28021 gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt
    28081 gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca
    28141 tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc
    28201 tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt
    28261 gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg
    28321 aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca
    28381 ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg
    28441 atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta
    28501 cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc
    28561 tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa
    28621 gtctcttatt ttaaattgaa tacattataa tgatactgta aatatatctt tattattagt
    28681 gaat
//
LOCUS       DACBZN010000025        28602 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.025, whole genome shotgun sequence.
ACCESSION   DACBZN010000025 DACBZN010000000
VERSION     DACBZN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.025"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            237..1877
                     /locus_tag="IVD64_001106"
     CDS             237..1877
                     /locus_tag="IVD64_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP6148369.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IVD64_001107"
     CDS             2031..3353
                     /locus_tag="IVD64_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6148370.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IVD64_001108"
     CDS             complement(3415..3846)
                     /locus_tag="IVD64_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6148371.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IVD64_001109"
     CDS             complement(3910..4746)
                     /locus_tag="IVD64_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP6148372.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IVD64_001110"
     CDS             complement(4936..5238)
                     /locus_tag="IVD64_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP6148373.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVD64_001111"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVD64_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP6148374.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IVD64_001112"
     CDS             complement(5692..5958)
                     /locus_tag="IVD64_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP6148375.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IVD64_001113"
     CDS             complement(6212..6937)
                     /locus_tag="IVD64_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP6148376.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IVD64_001114"
     tRNA            complement(7303..7373)
                     /locus_tag="IVD64_001114"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IVD64_001115"
     CDS             7490..8242
                     /locus_tag="IVD64_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP6148377.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IVD64_001116"
     CDS             8258..8737
                     /locus_tag="IVD64_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP6148378.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IVD64_001117"
     CDS             complement(8988..10568)
                     /locus_tag="IVD64_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP6148379.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IVD64_001118"
     CDS             complement(10561..11304)
                     /locus_tag="IVD64_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148380.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IVD64_001119"
     CDS             complement(11480..12256)
                     /locus_tag="IVD64_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6148381.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IVD64_001120"
     CDS             complement(12504..13340)
                     /locus_tag="IVD64_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP6148382.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IVD64_001121"
     CDS             complement(13351..14280)
                     /locus_tag="IVD64_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP6148383.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14473..14826
                     /locus_tag="IVD64_001122"
     CDS             14473..14826
                     /locus_tag="IVD64_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6148384.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14900..15556)
                     /locus_tag="IVD64_001123"
     CDS             complement(14900..15556)
                     /locus_tag="IVD64_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6148385.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15782..17716
                     /locus_tag="IVD64_001124"
     CDS             15782..17716
                     /locus_tag="IVD64_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6148386.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17802..19025)
                     /locus_tag="IVD64_001125"
     CDS             complement(17802..19025)
                     /locus_tag="IVD64_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP6148387.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19019..20917)
                     /locus_tag="IVD64_001126"
     CDS             complement(19019..20917)
                     /locus_tag="IVD64_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP6148388.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20911..22029)
                     /locus_tag="IVD64_001127"
     CDS             complement(20911..22029)
                     /locus_tag="IVD64_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6148389.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22112..22945)
                     /locus_tag="IVD64_001128"
     CDS             complement(22112..22945)
                     /locus_tag="IVD64_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6148390.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22938..23855)
                     /locus_tag="IVD64_001129"
     CDS             complement(22938..23855)
                     /locus_tag="IVD64_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148391.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24082..25206)
                     /locus_tag="IVD64_001130"
     CDS             complement(24082..25206)
                     /locus_tag="IVD64_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6148392.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25389..26297
                     /locus_tag="IVD64_001131"
     CDS             25389..26297
                     /locus_tag="IVD64_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6148393.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26438..28051
                     /locus_tag="IVD64_001132"
     CDS             26438..28051
                     /locus_tag="IVD64_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP6148394.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5621 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaaacaagt tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat
    14401 tgactttttc tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa
    14461 gggagaataa aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag
    14521 tcatccgttg ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt
    14581 ccacgactag aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt
    14641 gaaacaccag aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt
    14701 catcaaggaa aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat
    14761 ctactagatg aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca
    14821 aaataatcgt tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt
    14881 gttcaatcgc tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat
    14941 gcaagaaata gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat
    15001 ctgacggagc attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg
    15061 ttaactgatt gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga
    15121 tttgttggat cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta
    15181 cgatgcgccc aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac
    15241 tttgatggaa tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa
    15301 catttgtcaa ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat
    15361 cataaccata cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg
    15421 cactatctac ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat
    15481 tatttaaaat ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa
    15541 taatatgatc tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc
    15601 atcatagcag tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat
    15661 tctttattca tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga
    15721 ctactaattg aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc
    15781 catgatatta ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga
    15841 aaacatacat ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc
    15901 tggaaaatcg actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat
    15961 cgctaaaaat aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa
    16021 tgaaacagtt tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg
    16081 gatgcgcgag ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc
    16141 ttatttaaaa gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta
    16201 tgaaaatgaa attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca
    16261 gaaaatcgat actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt
    16321 gcaacaccct gacattttga ttctagacga accaacgaac catttagata tcgagacact
    16381 ttcctggctt gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga
    16441 ccgttatttt ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg
    16501 ccattacaaa ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga
    16561 atggaaggct tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg
    16621 gaaccttgtt cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa
    16681 aatggaccgg ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat
    16741 agaaaagcct tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa
    16801 tgtcacctta tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt
    16861 aggtccgaat ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt
    16921 tatccgtgga aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca
    16981 agctgatctg catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac
    17041 tccagaaaaa gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga
    17101 aaaaccaatc cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc
    17161 gatgaacaaa gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa
    17221 caaagaagta ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca
    17281 tgaccgatat ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag
    17341 caaagtctat ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat
    17401 cgccgaattg ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga
    17461 ttattaccaa agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca
    17521 ggttgaagaa gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt
    17581 cgcgccagaa aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc
    17641 tgctagacaa gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga
    17701 agaatatgaa aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc
    17761 tttttttatt ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa
    17821 ttactagtca ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta
    17881 tttaccccat tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct
    17941 tccgttgtag catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat
    18001 aagttctttt ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc
    18061 atgatcacca tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc
    18121 caataacaaa tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt
    18181 aaatcaaaat atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc
    18241 catgcttggc ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat
    18301 cttggttttc catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt
    18361 actaaataat ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt
    18421 ccagtttctt cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt
    18481 ctaccttgtt gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc
    18541 caaaaacgct gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt
    18601 cccgttattt tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga
    18661 ttagtgataa tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt
    18721 tgttgaaata aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat
    18781 ccttctgtcc aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt
    18841 gggaattgat actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa
    18901 cgctcgatct cattattcca ttctttttcc attaattctt tataacattc atttatccat
    18961 ttttctggat gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt
    19021 agcataattt tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat
    19081 caaaatcaat tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag
    19141 caaccactcc tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa
    19201 taaaagtgtt ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt
    19261 ttcgtttaat agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc
    19321 tatagttttc cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag
    19381 caaaccctgt ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct
    19441 gccagccttc aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg
    19501 tagaggcaag tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact
    19561 gtccataggt tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct
    19621 gaatccctag cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg
    19681 aaaaaccaaa ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga
    19741 aatttggccc taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga
    19801 gcaaattggc ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag
    19861 ccgtccaata ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata
    19921 atggagataa cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa
    19981 tattcaggat acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat
    20041 tgactaaccc cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg
    20101 accaaaagct tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac
    20161 catcttccgc aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata
    20221 tctggctaat gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg
    20281 caaaaggatt ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa
    20341 gataacaatt ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac
    20401 caaatttttg gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac
    20461 attcatattc aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta
    20521 attttttcca taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta
    20581 ccatacgctg gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc
    20641 cagcttttat tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg
    20701 gccccgctcc ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc
    20761 ctaccccttg atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg
    20821 gttgatttat ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg
    20881 gatgtttcat tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa
    20941 tcgaagtaag ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag
    21001 ttctctatgg tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt
    21061 tgcatagtgc ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga
    21121 tcgtacagag gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc
    21181 actggtatca atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt
    21241 ttcgattttc tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt
    21301 ttttgtgtaa cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca
    21361 acaagaattc tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc
    21421 atcaaattct gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt
    21481 atctgcatgc tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca
    21541 atacagtaat ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt
    21601 ccatgctact aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg
    21661 taaccctagt tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg
    21721 taagaataaa aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa
    21781 taattgttct tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag
    21841 ctgccataaa ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat
    21901 tacgtctgta tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa
    21961 ttccgcatct atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg
    22021 ttcattcatg gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa
    22081 cgaactagat ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct
    22141 ccttaaattt agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta
    22201 aaattagcat aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt
    22261 tgataattaa gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat
    22321 attgattcca acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa
    22381 tcggtacagc gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct
    22441 ccatcagtgc tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac
    22501 tggcaatcga agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa
    22561 tgtaaaataa aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat
    22621 tgaacaacag tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag
    22681 aactggctgt agctgaaact gtactgatta cagcaataac aatcgaattc caaataatca
    22741 agggataatt atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact
    22801 tgaaattgat tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat
    22861 ataaatatgg catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc
    22921 tgctttgtat cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat
    22981 aatataacag atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc
    23041 atttcaaaac ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca
    23101 ttggaagtcc caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct
    23161 aaaactgtgg ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc
    23221 cgaaatttcg atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga
    23281 agaccagcta aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc
    23341 attgatattc gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca
    23401 ttcaaaaatc cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca
    23461 gccaccgttg atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt
    23521 aactgtgctg aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt
    23581 gatcctgcca tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag
    23641 atagctagat aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta
    23701 aaagcaaacg agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt
    23761 gtgaccaatc cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc
    23821 tttattgtct tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta
    23881 atcaccagtt aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga
    23941 attttcaaaa tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc
    24001 atctgaataa gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag
    24061 ttcatagtat ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata
    24121 ggccaatgag ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc
    24181 tgtcagcata aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac
    24241 ataaaaactt gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat
    24301 cagtggagcc atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc
    24361 ataccagccg ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc
    24421 accgtagaaa tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt
    24481 cgcttcttta gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg
    24541 ggctagcatg ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact
    24601 accagttttt tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc
    24661 ttcaaaatct acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt
    24721 aaaatcacta gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa
    24781 tttttgatca taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata
    24841 aaaagcatct tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat
    24901 atccggcata tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc
    24961 cgaagctaca ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt
    25021 tgcagcattt tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt
    25081 ggcttttttt atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca
    25141 tgcacttaag ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt
    25201 tttcatgtct atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat
    25261 attaacggaa ttagtaacac aaatatttat ataaaaaaat attttacttg tcgttttggc
    25321 ttttcatttt tgttataata gtcattaatt ataaatagaa agctttttat ataaaaagag
    25381 aaaggaaagt ggttcttttg ttaacaaaca accaatcctc caatcttgtc aaaaaaacac
    25441 taacctattc catagatacc catcttcatt tatctgctgc tccctatttt cgactactaa
    25501 taaatgaaag cgctaattta tatgctgaat ttgcacataa atctatagaa ctagataatc
    25561 cttccctttt attcattgat aaacagcaag aagtccaaat cttttctact caaaaggaaa
    25621 cacaaacttt tatcattatc cagttttatt ttgatgagca attattagca catattacgg
    25681 gacaatcttt ttctatagat caagaaataa catctatcat tacttttctg aaaaatgaac
    25741 agttcaaaat tgattatcta aaacatagta agccagctat ttcctatgat caggaaatca
    25801 attctttttt catactatgc caacagatga gcattcattt gctttcctta ttagttactt
    25861 attgtctgca aaaaattcaa ttgtcttcct taccaaggca ttatcaacag gaacttgctt
    25921 tattgacaat accttcaaat gagaaagtca ttcctaaaaa aaagcatatt ctttattcag
    25981 atgaattagt tgatccaatc atacgatatt tagaagaaca tctttcagaa aattttttgc
    26041 ttgaacaatt agcccaaatt ttttttatca gtccagccag cttaaaaaaa cattttagaa
    26101 aagtgaatgg aaaaagtgtt attagctatt ttaaagaact aaaaatggaa aaagcaaaac
    26161 gtctcgtgct agaaaatgaa ttaacctata cagaaattgc agatatatta ggatttcatt
    26221 cgatccatca tttttcttca gcttttaaaa aatatacagg gttatcccct tcaaagtatt
    26281 atctatcctt aaaatagaaa aaaattttta ggttttatcc acaaattatt tctctatatt
    26341 ttctgtctat tcaaaataaa caataatttt ccggcacaat tatttgctgt tttttaccat
    26401 aacatagtac gatcaacgta gaaaaggaga tgtttttatg aaagttgttg tcattggcgg
    26461 tgtagctggc ggtccttcat ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat
    26521 cattatctat gaacgcggag aaaatatttc ttatgcaagt tgtgctttgc cttattattt
    26581 aggaggtgtg atcactgacc gtgactcgct gatcgaacgt acaccagaaa tattgaaaac
    26641 aaaaaacaac atcgatgtct ttactaaaca cgaagtaaca gcaatcgatc cttctactaa
    26701 gcgattaaca gttaaagacc tatccacaaa tgaagaaata aaaacagatt acgataagtt
    26761 gatcatctct tctggtgcta gaccagatta tccggatatt cccggagttt ttgaagcaga
    26821 aaacggcttt gtcctccgta gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga
    26881 aaaaaatcca caacatgtcg tcattcttgg tgcaggtgtt atgggtctgg aattagctga
    26941 gaatttcaag catcgcggct tagacgtgac tttaatcgat caattgccac aagtcgcttt
    27001 cccttatgat ccagaaattg ctaatttagt ttatgacaaa ttgctgaaag aaggattagc
    27061 cgttcattta gaaacaagag ttactgagat ccgtgataaa ggtcgagaaa tagtattatc
    27121 agatggctcc gtcctttctg ctgatatgct aatttttgct gttggtgttt ctccgaagaa
    27181 tgaagtggtg aaagcaaccg gcatacaatt atcagataca ggtcagatca ttgttgatga
    27241 ccagttacaa accaatctgc cggacatcta tgcgattggc gatattatcg aaacgactag
    27301 tgtagtgact ggtcagccga tccaaagtat gctttccagt gcggccaatc gtcaaggaca
    27361 tatgctggca gatattttaa atggtacgcc tatgcgctat cgcggatata ttggtgcagg
    27421 tgtcgcaaaa atctttgatc atacagcaag ttatgcggga atgacagaac atgaattaaa
    27481 agcatcaggc ataacaaatt ataaaactgt ttttatcact ccctttgacc atgcctattt
    27541 ctatccagaa gctacaagat taaatctaaa gctgattttt gatgcagata gcggtcgtat
    27601 tttaggtgga caagcatttg gagaaaaagg tgtcgataaa cggatgggag aactttctgt
    27661 agcaatcacc ggaaacttga cagtctttga tttgcctgat ttggagttgc cttactctcc
    27721 accgtattct actacccgtg acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat
    27781 gacgaatatc gtagaaacga tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc
    27841 gttcttttta gatatacgtg aacctaataa agcaccttct ggcagtatct cggcaacaaa
    27901 aaatattcca atgaatgagt tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt
    27961 ttacattact ttcagaagag gattgaatac ttatacttct gcccgaatct tggcaggttt
    28021 gggtatcaaa gcgattttga ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc
    28081 taggagttat ttttacttga ggagtaaaaa tgaaaaagta tatagggttg ttggttggta
    28141 tgtatataga atagaagata aatgtgaaga aattgtgcat tatttcttca catttattag
    28201 gttatagttt aagaaagttt aaacattgcg caatttaatt attttagctg tttcttttca
    28261 taaaaaaaca tgttgtgact ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa
    28321 ctacgcctaa tattacgagg catcatatta atctatcagc actagtacct aaactagcat
    28381 ccctttcgaa tattttttct tatataataa agtttgcttt cactcctcta atctcaccaa
    28441 atcgactggc ttgcgttgtg tgatactagg aactgattgg tttagaacga cttttctatt
    28501 gtaatcatat ttttcgccat cctcgttgtc ttctaatcct tcaatgaatg atccctaatg
    28561 tgtgtattaa ataatttaat ttttttgttc ctatttattt aa
//
LOCUS       DACBZN010000026        28010 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.026, whole genome shotgun sequence.
ACCESSION   DACBZN010000026 DACBZN010000000
VERSION     DACBZN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.026"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            226..894
                     /locus_tag="IVD64_001133"
     CDS             226..894
                     /locus_tag="IVD64_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP6148395.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IVD64_001134"
     CDS             complement(1094..1480)
                     /locus_tag="IVD64_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148396.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IVD64_001135"
     CDS             complement(1452..2834)
                     /locus_tag="IVD64_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6148397.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IVD64_001136"
     CDS             complement(2990..4177)
                     /locus_tag="IVD64_001136"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP6148398.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IVD64_001137"
     CDS             complement(4184..5215)
                     /locus_tag="IVD64_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6148399.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IVD64_001138"
     CDS             complement(5231..6685)
                     /locus_tag="IVD64_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6148400.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6860..7765
                     /locus_tag="IVD64_001139"
     CDS             6860..7765
                     /locus_tag="IVD64_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6148401.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            7856..8533
                     /locus_tag="IVD64_001140"
     CDS             7856..8533
                     /locus_tag="IVD64_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6148402.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IVD64_001141"
     CDS             8814..9710
                     /locus_tag="IVD64_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6148403.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IVD64_001142"
     CDS             complement(9765..11135)
                     /locus_tag="IVD64_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6148404.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IVD64_001143"
     CDS             11373..12119
                     /locus_tag="IVD64_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6148405.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IVD64_001144"
     CDS             12116..12955
                     /locus_tag="IVD64_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6148406.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IVD64_001145"
     CDS             12956..13906
                     /locus_tag="IVD64_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6148407.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IVD64_001146"
     CDS             14047..14799
                     /locus_tag="IVD64_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148408.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IVD64_001147"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IVD64_001147"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP6148409.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16354..16962
                     /locus_tag="IVD64_001148"
     CDS             16354..16962
                     /locus_tag="IVD64_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6148410.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            17130..18767
                     /locus_tag="IVD64_001149"
     CDS             17130..18767
                     /locus_tag="IVD64_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP6148411.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(18839..19951)
                     /locus_tag="IVD64_001150"
     CDS             complement(18839..19951)
                     /locus_tag="IVD64_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP6148412.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(20026..21399)
                     /locus_tag="IVD64_001151"
     CDS             complement(20026..21399)
                     /locus_tag="IVD64_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6148413.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            21620..22237
                     /locus_tag="IVD64_001152"
     CDS             21620..22237
                     /locus_tag="IVD64_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148414.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            22335..22790
                     /gene="argR"
                     /locus_tag="IVD64_001153"
     CDS             22335..22790
                     /gene="argR"
                     /locus_tag="IVD64_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6148415.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            22808..24310
                     /locus_tag="IVD64_001154"
     CDS             22808..24310
                     /locus_tag="IVD64_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP6148416.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            24405..24611
                     /locus_tag="IVD64_001155"
     CDS             24405..24611
                     /locus_tag="IVD64_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148417.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            24697..25098
                     /locus_tag="IVD64_001156"
     CDS             24697..25098
                     /locus_tag="IVD64_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6148418.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVFEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            25253..26584
                     /locus_tag="IVD64_001157"
     CDS             25253..26584
                     /locus_tag="IVD64_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP6148419.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            26606..27232
                     /locus_tag="IVD64_001158"
     CDS             26606..27232
                     /locus_tag="IVD64_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6148420.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            27415..27996
                     /locus_tag="IVD64_001159"
     CDS             27415..27996
                     /locus_tag="IVD64_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6148421.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
BASE COUNT         9334 a         5147 c         5730 g         7799 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggatctactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacaaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccacagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagaat gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa aacatagaaa aacgattact ttgcttatac aacaaggctt ttatggtttt
    16201 gtatcaaaaa aatatttgtt taaatgttac aaatattaaa taattcgtta ttttgtcatt
    16261 aaatgtcatg tttcttttac ctatgaaata tttctaaatg ataaagttaa tagcgtcgaa
    16321 agaataaaaa acggagaaaa aaaggagttt aatatgaatt catttaagaa aattgtttta
    16381 ggtacaactt ttgctgctgg tgctactgca atgtttgtag gaactacaaa tgctcacgct
    16441 gatgaagttt atacagtaaa atcaggtgat tcattatcta agatctcaca aaaatttgca
    16501 ggagacaact caatgatcga tgcaattgct gaaaaaaatt cgatcgcaaa catcaaccgc
    16561 atttatgttg gtgaacaatt aacaatccca acttcaaatg attcttcagc aacaacagaa
    16621 aacaaaacaa cagaaaatac agcatctaca actgaaacag caacgcaaga acatacatac
    16681 gttgcacctg ttgaaacagt tgaagtagct cctgctgccc cagctgctgc aacagcgcca
    16741 acttcaagct ctgcaaaaga atggatcgca caaaaagaat caggcggttc atacacagct
    16801 actaacggac gttacatcgg ccgttatcaa ttagatgctt catacttaaa cggcgactat
    16861 tcagctgcca atcaagaacg tgtagcagaa caatatgtaa catctcgtta tggttcatgg
    16921 gatgctgcta aaacattctg gttagcaaac ggctggtact aagccgataa aaggaaaatg
    16981 atgaaaccga gaaacttgtt tattcaagtt ctcggttttt ttgtgtgaaa aataactcgt
    17041 ttgcttccca cagcgcagca gtacaaataa aaactcgaat aatgtataat aaagaaaacg
    17101 tttttttgtt tagggaatga ggaggagcca tgagtacaaa agagagtctc gaattaaacg
    17161 aaatcacaag tggaaatcca ctgttatcca gtattcgatc gattgtagaa acgacatttt
    17221 acggtaataa tgttcatgaa gtttttgata ggaagacagc ttatcaacta gcaaaaggga
    17281 gtccaggaac gatcatcaca gatttaacaa tctcacatgc tgaagaacta gatttaccag
    17341 ctgatgttag gacactagta ttcaatgatg gaagcattgt gggaagaaca gcaagtgctc
    17401 ggcggatctt tgaggactta gacaaggaac aaagcaaata cgagaaaatt ttaagagaag
    17461 ctgtgtatca aagtcgcaag cgccagtttt accatacaaa agtaatcgtt ggcctatctg
    17521 aagaattttc tgtgcaaagt catttgctga ttccagaagg atttgagaac aatttgtatt
    17581 cttatttgct gaattttcag ccagcgaatt cgaagatctt agagacttat caaaagtctg
    17641 tttcctttga tgagggagat ctttatatct tttgtgatcc cgaatggaca catgaaacat
    17701 ttccaaatgg actcattttg tttgatgctc tccataatac agcggttgtg ctaggattac
    17761 gttactttgg agaattaaaa aaagcgactt tgacgcttgc ttgggcattg ggaaatcgca
    17821 atggctttat tgcctgccac ggtgggatga aacaatatgc tagtaatgac cagacctata
    17881 cgatggctgt atttgggttg tccggttctg gaaaatcaac gattacctta gcaaaacata
    17941 cggaaggtca ctctgtttcg atcttgcatg acgatgcttt cgttatttct cgaaaaaatg
    18001 gttttgctac tgccttggaa ccttcttact ttgataaaac acaagactat ccgtctgatg
    18061 atccggccat ttcttctttc cttacttgtc agaatgtggg cgtgacatgt acaagtaaag
    18121 gagaaaaagt cctagtaaca ggagatatac gaaacggaaa tgggcggaca gtcaaatcac
    18181 gttatgcgac acccgaccga gtcgatcatc ttagcgaaaa gatcgatgct gttttttgga
    18241 tcatgaaaga tgattcgctt ccgccaatta tcaagatcaa tgacccagtt ttagcagcgt
    18301 cttttggtgc aacattagca acaaaacgat cagacgcgga aaatatcact acaaaaagac
    18361 agctagatga gctagtcatt gaaccatttg ctaatccttt ccgtgtctat cctcttaggg
    18421 aagactatca agcttttcga gagttgtttt tgaaacagca aacagcctgc tatgtcctga
    18481 atactggata tttcgatgat aaaaaaatca aacctgaaac gacattgtca atcgttgaaa
    18541 gaatcgtgca acaaacggca gaattcaagt cgtttggtcc atttgaagac atccactatt
    18601 tacctatacc agattttcct gtttcttttg aggatacgtc ctataaaaat ctggtgaaac
    18661 aacgacttga tattcgttta cgattcattg aagctcagat aggaaaagat ctcttgccag
    18721 atgaagcaaa agaaagtttg gagaatttga ttcagaaatt gaactaaaat aacagctttt
    18781 tcaagaagta atccatgcgc aatatcgctc gggatcctta cttgaaaaag ctgtttttct
    18841 atgatggatt tttcgtaata taggtggaga tgtccttttg ataatcttcc gtaggccaat
    18901 gaccagtatt taagaagttg gctaatagat agccgatata aggacctgtg gtcaagccag
    18961 aagagcctag accgctagca gccagtaagc ttggatcatc tggcagtacg ccgaaaaatg
    19021 gggaaaaatc tgacgtataa gcacgggtac ctacacggaa ctcgtatggc cagtttttta
    19081 tctgatctgg atcagtcaag aactgttctg tactcttagt caatccctta taagcttcgc
    19141 tagtaggagc caaatcccag ccttgatcgt tttcatgggt agcacctaaa aggatctttc
    19201 catccatgaa aggaatgaaa tcagcttctc cgtcaagcat agcaacaggc cagtcttgac
    19261 tcatcttata agatgtctga aatgctaaga gctgcccttt ttgcggacgt atatctgcag
    19321 catagcctaa ttcttgtaat aaaggcttaa gtgccgggcc aggtgtcaaa attacacgat
    19381 cagctgtgta ttctccaaac ggtcccttga tgagccaaga atcgctcttc ttatgcaaag
    19441 tgacttttgc ctggatctgc tccatttgtt tttgtgctaa acgctgacca agatgttcta
    19501 aatatgcttt tccatccaga cgaccaccac cactgatacg cagtgaaact gctggtttca
    19561 ataacggaag tgcttgagat gtctgttcag gtgaaagcca ttcgatgttt ccaatttctg
    19621 gcgcttcttc ttttcgtttc tcggcaagaa cagccaattc agctaaatct ttttcaggtc
    19681 gaagcaaaag tgtccctgat tgtgcgtaaa tcgtttgatc aagttggaaa tcttttataa
    19741 gtttagggaa aaaagcagcg ccgtctttag ccagacgata ccattgtttg tttctgcgtt
    19801 tcgacaacca aggggagata atcccagcgc tagctttagt tgcctgtcct gtcccttcat
    19861 caaataaagc aacattgaat tttgatgtat ctaagtaact ggctagggtc ataccaatga
    19921 tcccgccacc gataatggca attttttcca taaaaaagcc tccttttaca aacaaccatt
    19981 ttcacacaaa aaaacggcag atgcaacacc tgccgtatat ggaatctacg cttttttagc
    20041 aaagaacaga taataggcga atcgagcaaa gcttacgaga agcaacagca acaagacgcc
    20101 taatgctgct tttgatccta atgtagtaga cacttcatag ttatgggaag tatgatgctg
    20161 gacgtaattg ataatgaccg ctactaatga agtagccaca gcgccagaaa actgctgcaa
    20221 ggtattgaac attgcgttcc cgtctccttg tagttcttca ttcagtgcat tcattccggt
    20281 ggtcatcata ttgctgtaag ataatccgat accgatcata taaaacacat gacaagcaat
    20341 caatacaccc actgaagcgt gtcctatgag taggaataat gctagccaac caatcaaaga
    20401 aagaatcagc ccagcaaaaa ttggcttctt cggaccatat tgatccagca ttttcccgga
    20461 aaaaggagaa agcaaagcac caaccgctgc gccgggaagc atcgctaatc cagcgacaaa
    20521 aggactactt ccttgaacga tctgaaggaa attcggcaag acaaaagata caccaagtaa
    20581 taaaaactga taaactaaga agccaatcaa aaacaagcgg aatgttttat ttgaaagaat
    20641 agacagtcgg atcagtggat gctccacttt tttacttcga tagtaaaaaa ggaaccagcc
    20701 aataccgccg atgattaaag caatccagct ggaaactgtt cccaacgtac tcaaaaatgt
    20761 tagacttcca ctaaacaaaa gacaaattcc taaaacgctt atccaatcca aataaccact
    20821 tttttgtact gccatctcgg gaatagcaaa ccagccgata atcaaagaaa gaatcaaaac
    20881 gggcacaaga aacaggaaga tatgattcca tgtaaaatgt gaagtcataa tgcctccata
    20941 agtggggcca attgctggag caatggccgt tgtcaaagta ccgacaccca tcatcgcgcc
    21001 tcgtcgattg aggggggcaa atgtcaagat aatatggaac atcaacggaa gagcgatacc
    21061 tgtcgctgca ccttggagca atcgaccaaa caataggacc atgaaggtag gagagaagaa
    21121 atcgatcatt atacctgcca agaaaagcag atttgaagct aaaaagagtt ttttgagact
    21181 aaaattgcga ttcaaataag tagacagcgg aacaatgatc gaaatcatca aaagataaat
    21241 cgttgttacc cattggactt gtgcagtcgt taaatcaaac tgtctgataa gagttgggaa
    21301 agttacattc atggccgtct cgatcaatac gcccgcaaaa gacatcatcc ctgttgcaat
    21361 tactgcaggt actaaaagca gctgtttttg ttttgtcatt tacatcacct ctaaaaaatt
    21421 tcgtaaaaaa agagagtgat ttctattata aaaatagaaa ccgcctctct ctcgacacgc
    21481 cgaaacgtgt tatctgtacg atttatttac ttcacttatt ctagcacaaa ctgaaaaata
    21541 gtgtcaacac gattagccaa gtcactcaaa aagaggtatg ataataaata actaaatgga
    21601 atttcgcgga ggaagaaaaa tggaaaaaat tgaatggcgt aaagacaaga gctatagtat
    21661 gaaaaaaagc ccgcaaatac tagaggttcc taaaaaatca ttctttagca tcagagggat
    21721 cggcgacccg aatcaggaag actttaaacg aagagtagcc tgtctatatg cagtcagcta
    21781 tgcaatacgc atgtcgccta aaaaaaattg gctgattcct gattatcaga tgtatactgt
    21841 ctatccactg gaaggacaat ggggactgca agaaaagtat ttgcatgaac cagtgatgaa
    21901 aaaagaacat ttttcttatc aattgatgat ccgacagcct gactttgtta cagaagcgat
    21961 agcacaagaa gccattcagc gttctagcaa acttccggaa gacttgaaag aacaagtagt
    22021 attcaaaaag atggaagaag gtctttgtgc ccaaatctta cacgtcgggc cctatgccga
    22081 agaaccagca acgtttgaga aattagaagc attcatcgca aaagaaggct atgaaagagc
    22141 ttctaaagaa cacaaagaga tttacatcag cgatccaaga aaatcagcac ctgaaaaaat
    22201 gaagacgatt ttgcgtgtta aaatagaaaa aggttagcaa cctgaatgaa aacaaaaaat
    22261 aaaagtaaaa aataccaaaa tatgaagaaa tattacttat cttttgctat aatcaacatt
    22321 gagaggtgtc taatatgaaa aagaaacagc gacaagcact gatccgccag atcattacag
    22381 aacaacctat tgggacacag gaagagctgc ttgcgcggct gcatgaagca ggcgccgatg
    22441 tgactcaagc aacgatttct cgagacattc gagaaatgaa gttagtaaaa agtcaaaatg
    22501 aaaacaaaat agtacgctat acattattta accagccatc tgtttcttta aatgaagaac
    22561 gtttgcgtac agcaattaga cgcgaggttt tgcgtatcca gtctgttcag ttcatggtta
    22621 tcgtattgac tgaacgaaat ggtgccgatg tggtaacgaa ttggctagat gaagtggctt
    22681 accctgaagt tgtcggaacg atggccggtg tagatacatt cattattatt tgccgctcag
    22741 aagaagaagc acagcgattt gcagaaaagc tggaaaaaat gagggaataa atgaggagga
    22801 cttttatatg aaagatatga aagcgctgca aaatggatcg gatattcgag gaatcgcatt
    22861 accagttgaa ggatatgaag taaacttgac gacagacatg gtaaaaaaga tcggctgggg
    22921 actagcaaac tggataaaga aaaagaagaa atcaaccgat ccaaataaaa aatggacgat
    22981 tgggattggt catgacagta ggataagcgg accagcactg aaagaggcgt tgatccaaag
    23041 tctgaaagaa caagggatac atgtcatcga tttcgaatta gctactacgc ctgctatgtt
    23101 tatgtccact caatttgaac agttctcgtg cgatgctgcg gttatgctga ctgctagcca
    23161 tcttccctat tattataatg gaataaaatt ctttacctca gaaggaggag cagaaaaaga
    23221 agatattgct tttatcctat cacagcagca aaaaataaaa agcgaacaaa ctggaagcgt
    23281 agaaaaagca gaccttttga caccttatgc gcaagattta gtcacaaaaa ttaaaaaggg
    23341 gattggaaca ctagaaagca aaccactaaa tggttggaaa atcattgttg atgccggaaa
    23401 tggtgctggt ggattctttg ctgaaaaagt attgaatagc ttaggtgcgg attcaacagg
    23461 ttcccaattt ttggaaccag atggccgatt tcctaatcat atcccaaatc ctgataataa
    23521 agaagcgatg gaaagtatca agcaggctgt tttagcaaat gaagcagatc ttgggatcat
    23581 tttcgataca gatgtggatc gttctgcagt tgtcagtgct tctggaacag tgatcaatcg
    23641 aaataatttg atcgcagtat taagtacaat cgtattaaaa gagcatccag gaacaagaat
    23701 cgtgacgaat tcccctactt cttctcatct acaaagattc attgaagaaa aaggaggcat
    23761 ccaagttcgt tatatttccg gctatcgaaa tgtaatcaat aaagcattag aacttaaccg
    23821 tcttgggaaa gatacacagc tagccatcga aacaagcgga cacgctgcat tcaaggaaaa
    23881 ctattttctg gatgatggtg catatgtcat cgcaaagatc ctgatgcttt tgccagaatt
    23941 aaaaagaaaa ggtatcacat tagatgaaca aatcaaagag ttgaaacagc ccgtagaagt
    24001 acaagaaatc cgattaaaat tgttcagcga tgaaccacgc gaacaaggaa acaagatatt
    24061 agaagaaatg aagcgactgt tggaagaaga aaaagggatg aacttcgacc cggaaaatga
    24121 agaaggtatt cgatatgatc tagaagagcc atacggaaac ggctggtttt tgatccgttt
    24181 aagcttacat gagccgttac tagtattaca gatagaaaat gatgaattcg gaaagaacaa
    24241 gttggcgtta gtaaaattag caaaattatt cggacatttt tctgatcttg aatcattaac
    24301 agcagaataa accagtcttt ttgaaaggct agttgcttgg acggcaaaaa aagtggtatg
    24361 gttaaatcga gtgctggtag ggagaccagc aaaaggagga aaaaatggta acgaatatgg
    24421 ttattttttt aacagtattg gtttatgggg tcaactttct ggtatatatg tatcttaaaa
    24481 tcaatcatat aaaagattat gtagaaaaga ttgctattta ttttggaaca aatatgacgc
    24541 tgttgttcac gagtagtatt tttttgtttt ttggaaaaat agtagaagac ggcattctgg
    24601 tattcgaata agcagaattt ttgtagaaag cgccttactt gtcatacact ataattatag
    24661 ccaagtagca aatggtcaat cgaaaggagc agtacaatga aaaaagcata cgaaacagca
    24721 atgatcaacc atggcgggcg caatggagaa gtcgaggcac caaatggctc gatgcatatg
    24781 aaaatcactc ctccaggaat ccatgcagaa ggaacaaatc cagaacaatt atttgcagca
    24841 ggttatgctt cttgtttcaa tggggcggta caacatatga tcaaagaagc aggaatgaca
    24901 gcagagtcca cagtaaaagc acgtgtctct ctttataatt tagatgatgg cggctatcag
    24961 atcggtgtag tattcgaagt gcatatcgat ggcatcagcg aagaagaagc ccaaaaaatt
    25021 gcagaagatg cgcatgaatt ctgcccttac tccaaagcaa ctagaggaaa tatcgatgta
    25081 gaagtaacga ttgtttagaa gaataaagga aatcgaacca gactaaaaac gtgaaaggac
    25141 cgctttttgt ctggtttttt ttgacaaata aagcagatga tttggagaat agacaattat
    25201 cggttatcat atttttgtac caatcggtag gtaaaaaaga agcgagggaa aaatgatgga
    25261 aaaatatcaa aatattgtga tcggttttgg aaagggcggt aagactttag cgaaggcgtt
    25321 agcttcaaaa ggtgaatcgg ttttagtcgt tgaaaagtca acacgtatgt atggcggaac
    25381 atgtatcaac atcggctgta ttccttctaa atcattgatt ttcaatggag aaagaggaat
    25441 cgactttaca gaggctgttg ctcggaaaga aaaattgacg ggcatgcttc gcgccaaaaa
    25501 ttatcatatg atcagcgatg aggcaactgg gacagtgatg gacggtacag cacgttttct
    25561 ttctaaccat caaatcgaag tgacgaataa tggtgaaaaa gtgattgttg aaggcgaacg
    25621 gattttcatc aatactggtt ctgaaccaat cattttacca atcaaaggat tgaatacaag
    25681 tcgctacttg attgattcta cgcaagcgat ggatcaggaa caattaccgg aaaaattagt
    25741 gatcatcggg gctggatata ttggcttgga atttgcatca atgttcaatg aatacggatc
    25801 taaagttgtc gttcttgatg cccattctga atttcttcca cgtgaagatg aagatatcgc
    25861 gcaaatgatt ttagaagaca tgacaaatgc gggaatcgaa ttccatctag gtgtatctgt
    25921 agatcaagta gcagaccaag aatcaacagc tgccgtcacc tttacagaag atggccaaga
    25981 agtaacaatc caagcgtcaa aagttttggc tgcaactgga agaaaaccaa gtacagcatc
    26041 acttggatta gaaaatacag atgttaaact aacagatcgc ggagcaatcg ctgtggatga
    26101 ataccttcgt acagatgcag acaacatttg ggcgattgga gacgtcaaag gcggattgca
    26161 gttcacttat atctctctag atgattatcg aatcattatg gatcagttga agggcgagaa
    26221 taaacgaaca acgaataacc ggaaagctgt gccttacagt gtatttatca ctccgacttt
    26281 atccaatgta ggtttaacag aaaaacaagc caaagcacaa ggcgtcaatt acaaattatt
    26341 caagtatatg acaagcggtg tccctaaagc acaagtatta gaagatccta aaggtgtatt
    26401 caaagtttta gtcgaccctg aaactgactt gattctaggg gcaagtatct atgcggaaga
    26461 ttctcatgaa gtcatcaact tgatttcgct agctatgaat ggaaaacttc cttatacgtt
    26521 attgagagat caaatctatt ctcatccgac aatgagtgaa gctctaaatg atgtattaaa
    26581 ataaataacg aatggagaat ccaccatgtt caataataaa aatacagcag tagttctcac
    26641 tgacccgcaa gtcgagttct tgaaaccagc aggcggtggc tttggtttga caaaagatat
    26701 cttagaaaaa tacaatacga tcgaaaatat gattgaaatg gtaaaacaag caaaagaaaa
    26761 aggatacaaa ctctttattt ctcctcatta tttttaccca catgaccata aatggcaatt
    26821 tggcggtgca ggagaacata tgatgcttga gcagcagatg tttgctagaa aaagtcaata
    26881 tgaatcagtg gaagaaggtt caggagccga ttttatcgaa gaattgaaac cttatttgga
    26941 tgaaaatacg atcatcagct ctcctcataa aatctttgga ccagaaagca atgatttggc
    27001 gctccaattg agaaaaaatg gaatcgacac agtaattctt ggtggaatga acggaaactt
    27061 gtgtagcgat tcccatatgc gtgaattggt agagagtgga ttcaatgttg tggtagcgac
    27121 agatgcaatt ggtgctcctg gccaagaagc aatggatgca gcgttgacga actttggctt
    27181 tattgcagat caagtgatga caacagagga agtattagag caactatcat aagataggaa
    27241 ttgcttttgt cctcgcagtt cttgaatcaa tgaacatcta gctatatgag aacagtttta
    27301 tagtgagggc tgtgtcagat aatttggcac agtctttttt aaacgattaa gaagaaaaaa
    27361 tgatgaattt actgtataat ataggactat gagaaatgaa aaaggagaat atcattgaaa
    27421 acaaaggaaa agatcttgga agcgaccaaa gatcttattt ataaaaaagg ctatcatgaa
    27481 acgtcaatcc gagatattct agcagcttct gatacaggaa aaggtcaatt ttattattac
    27541 ttttcttcaa aaaaagaagt ttgtctagcg gtgatccatg cgcatatgga cgtatggcag
    27601 acagaattgt tcgaagacat cttaaatgct ggaaaacagc cagaagaagc attagaagaa
    27661 atgttggact ggattttcgc tgatcatgag acacagatca cttatcatgg ctgtcctgta
    27721 ggtaatttga ttattgagtt gagtacagtg gatgaagaat accgtctgtt gctactagaa
    27781 ttagtcaatc aatgggtcaa gtatacgaaa caaaatatcc tggcactttc tgaagaaaaa
    27841 ctgtccgatg aagaagcaga ccgtcaagcc aaagcgttga ttgctgaaat ccagggaagt
    27901 atcttattgt tgaaagtcac acaggatcta tcactactta ggcagaattt tcaattggtc
    27961 aaagaaagat tgttaacttc gcaaaaggtt ctttaataat tagaggttgt
//
LOCUS       DACBZN010000027        27635 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.027, whole genome shotgun sequence.
ACCESSION   DACBZN010000027 DACBZN010000000
VERSION     DACBZN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.027"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            121..768
                     /locus_tag="IVD64_001160"
     CDS             121..768
                     /locus_tag="IVD64_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP6148422.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            852..1796
                     /locus_tag="IVD64_001161"
     CDS             852..1796
                     /locus_tag="IVD64_001161"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP6148423.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1931..3382
                     /locus_tag="IVD64_001162"
     CDS             1931..3382
                     /locus_tag="IVD64_001162"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP6148424.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3403..4239
                     /locus_tag="IVD64_001163"
     CDS             3403..4239
                     /locus_tag="IVD64_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6148425.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4239..4898
                     /locus_tag="IVD64_001164"
     CDS             4239..4898
                     /locus_tag="IVD64_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6148426.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5024..5713
                     /locus_tag="IVD64_001165"
     CDS             5024..5713
                     /locus_tag="IVD64_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148427.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5719..6675
                     /locus_tag="IVD64_001166"
     CDS             5719..6675
                     /locus_tag="IVD64_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP6148428.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6719..7231
                     /locus_tag="IVD64_001167"
     CDS             6719..7231
                     /locus_tag="IVD64_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6148429.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7487..8035)
                     /locus_tag="IVD64_001168"
     CDS             complement(7487..8035)
                     /locus_tag="IVD64_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6148430.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8124..9305)
                     /locus_tag="IVD64_001169"
     CDS             complement(8124..9305)
                     /locus_tag="IVD64_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP6148431.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9541..11049)
                     /locus_tag="IVD64_001170"
     CDS             complement(9541..11049)
                     /locus_tag="IVD64_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP6148432.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11208..11399
                     /locus_tag="IVD64_001171"
     CDS             11208..11399
                     /locus_tag="IVD64_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148433.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11602..12519
                     /locus_tag="IVD64_001172"
     CDS             11602..12519
                     /locus_tag="IVD64_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP6148434.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12512..13585
                     /locus_tag="IVD64_001173"
     CDS             12512..13585
                     /locus_tag="IVD64_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148435.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13733..14251
                     /locus_tag="IVD64_001174"
     CDS             13733..14251
                     /locus_tag="IVD64_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148436.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14232..14624
                     /locus_tag="IVD64_001175"
     CDS             14232..14624
                     /locus_tag="IVD64_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP6148437.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14700..15635
                     /locus_tag="IVD64_001176"
     CDS             14700..15635
                     /locus_tag="IVD64_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP6148438.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15768..16547
                     /locus_tag="IVD64_001177"
     CDS             15768..16547
                     /locus_tag="IVD64_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP6148439.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16540..16857
                     /locus_tag="IVD64_001178"
     CDS             16540..16857
                     /locus_tag="IVD64_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6148440.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17101..18711
                     /locus_tag="IVD64_001179"
     CDS             17101..18711
                     /locus_tag="IVD64_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6148441.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18868..19716
                     /locus_tag="IVD64_001180"
     CDS             18868..19716
                     /locus_tag="IVD64_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6148442.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19947..20426
                     /locus_tag="IVD64_001181"
     CDS             19947..20426
                     /locus_tag="IVD64_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148443.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20459..22687
                     /locus_tag="IVD64_001182"
     CDS             20459..22687
                     /locus_tag="IVD64_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6148444.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22816..24459
                     /locus_tag="IVD64_001183"
     CDS             22816..24459
                     /locus_tag="IVD64_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6148445.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24532..25077)
                     /locus_tag="IVD64_001184"
     CDS             complement(24532..25077)
                     /locus_tag="IVD64_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP6148446.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25100..25585)
                     /locus_tag="IVD64_001185"
     CDS             complement(25100..25585)
                     /locus_tag="IVD64_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6148447.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25575..26387)
                     /locus_tag="IVD64_001186"
     CDS             complement(25575..26387)
                     /locus_tag="IVD64_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP6148448.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26526..>27635)
                     /locus_tag="IVD64_001187"
     CDS             complement(26526..>27635)
                     /locus_tag="IVD64_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6148449.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9048 a         5028 c         5526 g         8033 t
ORIGIN      
        1 tctttttctt tatattttca taataaaagt ttggttcttt aatttttaaa catgacagaa
       61 aattttttct tcgttatact tgtaatagaa tactatttta taacaaaggg gatcgaatca
      121 atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa
      181 tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca
      241 caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct
      301 agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc
      361 caagataaat acccagatac tgcaattact agtttggaat tagatagtga ttggggatct
      421 tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct
      481 caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt
      541 caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca
      601 aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat
      661 ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag
      721 atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg
      781 aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag
      841 gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga
      901 tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag
      961 aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac
     1021 cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt
     1081 tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac
     1141 cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa
     1201 tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg
     1261 gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca
     1321 gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg
     1381 atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg
     1441 tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag
     1501 ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt
     1561 tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac
     1621 aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt accgagcttc
     1681 ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag
     1741 aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa
     1801 gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc
     1861 ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa
     1921 ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag
     1981 ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa
     2041 tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc
     2101 agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa
     2161 gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc
     2221 taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc
     2281 agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg
     2341 gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg
     2401 aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt
     2461 ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg
     2521 gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga
     2581 ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt
     2641 tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc
     2701 gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct
     2761 tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat
     2821 cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac
     2881 aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca
     2941 atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc
     3001 agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc
     3061 atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga
     3121 acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga
     3181 gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag
     3241 cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag
     3301 aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca
     3361 tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg
     3421 tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat
     3481 caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct
     3541 ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta
     3601 gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac
     3661 gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt
     3721 ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt
     3781 gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa
     3841 gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga
     3901 gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat
     3961 ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta
     4021 ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt
     4081 ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg
     4141 aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc
     4201 aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt
     4261 tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga
     4321 ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca gttttaggtc
     4381 ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca
     4441 ttcccgcact agtcctgctg tttctgttat attttgggtt accttatcaa ttaagtgcct
     4501 taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc
     4561 gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt
     4621 ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg
     4681 cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag
     4741 tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc
     4801 tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac
     4861 agtatttaga aaaacgttct atgcatcagt caatctgaaa aatagaaatg gtgctagcgt
     4921 ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt
     4981 tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac
     5041 catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga
     5101 agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt
     5161 tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc
     5221 tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata
     5281 tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac
     5341 ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa
     5401 agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga
     5461 agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag atcaattgta
     5521 caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga
     5581 acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc
     5641 gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga
     5701 gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta
     5761 gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa
     5821 ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat
     5881 cgaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg
     5941 cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa
     6001 aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac
     6061 ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca
     6121 gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt
     6181 caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac
     6241 tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc
     6301 cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat
     6361 gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac
     6421 caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat
     6481 catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa
     6541 ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt
     6601 cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat
     6661 gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat
     6721 gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc
     6781 agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa
     6841 agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca
     6901 aattgctgga acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg
     6961 tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag
     7021 tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag
     7081 caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac
     7141 catctattct gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga
     7201 aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga
     7261 cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc
     7321 agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag
     7381 cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct
     7441 tttgtcacga cctctcggct tatcctacga gtattattcg atacatttat atcaataatc
     7501 ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg
     7561 aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg
     7621 ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag
     7681 agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac
     7741 catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt
     7801 tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga
     7861 tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag
     7921 gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg
     7981 cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca
     8041 tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa
     8101 aagtaacttt gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag
     8161 ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt
     8221 tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat
     8281 gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt
     8341 gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg
     8401 gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa
     8461 cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca
     8521 atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca
     8581 agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc
     8641 tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca
     8701 aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat
     8761 ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc
     8821 tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata
     8881 gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct
     8941 agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata
     9001 gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt
     9061 gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac
     9121 atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca
     9181 agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta
     9241 ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa
     9301 ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata
     9361 ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag
     9421 aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa
     9481 aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa catttataag taaacttttt
     9541 ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat cgattcctag
     9601 ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg cttgtgctcc
     9661 ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta ctgcactgat
     9721 tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat gaagtgtctt
     9781 tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa tgtcatctgc
     9841 gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat ccatcaatga
     9901 acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca cagcagaagg
     9961 atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag ggactaccac
    10021 gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc gattaactgc
    10081 ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac gacggatgat
    10141 ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc ctacacgatt
    10201 cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt tttcaataat
    10261 acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta ctttattgta
    10321 ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg tggctgcttc
    10381 ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag tttcgaactt
    10441 ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca aggaagtagc
    10501 gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag ttgtttcaat
    10561 atactcattt tttccaaaac catcttctga gacgattttt tctacgttga ctggatagtc
    10621 ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat tgataaagac
    10681 tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta ataagccata
    10741 accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc cataatcacc
    10801 acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt cagcttgatc
    10861 tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa gtacacgttt
    10921 cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt catagtggtt
    10981 cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat caaaaccaat
    11041 ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt tgaggattcg
    11101 tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct
    11161 agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg aaaaaaagac
    11221 agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt tatgaaagaa
    11281 tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa gaaacgcagc
    11341 taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct agtaaataaa
    11401 aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga ctagttgatc
    11461 gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg cccgctcttt
    11521 tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg aaaaacagat
    11581 gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta tgagccagac
    11641 gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc tttttacgat
    11701 atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca ggcttttgaa
    11761 aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg ccgattgaat
    11821 gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct gatgcgtaag
    11881 atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa catctcgcca
    11941 acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt cggtattgat
    12001 gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga gaaatattcc
    12061 caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact
    12121 gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg acaaatgact
    12181 gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa
    12241 ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc ttatattgag
    12301 acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag agcagaccag
    12361 cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt cggtagcaaa
    12421 gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa tgagaatcct
    12481 aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag cagaatggac
    12541 aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg aagaacaaac
    12601 aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa aaatacacga
    12661 aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg cttacgaaat
    12721 aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata tcgtcagttt
    12781 gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt tgatcgaatc
    12841 agattttcca caattattag aatcatatga tcgttttata ttgaatgaag aaccaatctc
    12901 cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga aaccgttgaa
    12961 tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa gacatttttc
    13021 gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat taccgaccat
    13081 cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag agacaatgga
    13141 aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag tggatcgagt
    13201 attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat tagcccatgc
    13261 aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc cacttcgttt
    13321 gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt ttatcgtggc
    13381 aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc aagtacgatt
    13441 agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat
    13501 ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg aattcttggt
    13561 cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt cattcagata
    13621 attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt cttttctgta
    13681 tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag gaatgtccac
    13741 aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt
    13801 tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag tactttactt
    13861 aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca gtttttattt
    13921 tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc tgagtttgtt
    13981 ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg gagtctgttt
    14041 ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc tgttatatcg
    14101 tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga tgcacagtat
    14161 aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt acaatcattc
    14221 aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc agtatactca
    14281 ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag gaggaagtaa
    14341 aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag
    14401 aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat tttgaagtca
    14461 aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag aaaaatcaac
    14521 tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa gagatcaaat
    14581 tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca taaaaaactg
    14641 ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag gaaaataaca
    14701 tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg gctaatattc
    14761 tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc atgctaaacg
    14821 aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc ggtgataaac
    14881 aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca aaatactttt
    14941 tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg gtgatggaag
    15001 cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt gttatagcaa
    15061 tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca
    15121 aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg
    15181 aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc ttggctttag
    15241 caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc agcggattgc
    15301 tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt tatagtctga
    15361 tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct tttgaacgtc
    15421 ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg gagattttag
    15481 ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg gtcatcaatg
    15541 atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata tacattgaaa
    15601 cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga attgaagcaa
    15661 aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc gaatttatgt
    15721 tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga
    15781 aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata agctatatac
    15841 cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct ctcttatcag
    15901 gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca tctatgctta
    15961 cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa ttacgctata
    16021 cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg tttttggctg
    16081 tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag ttttcaactg
    16141 acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg actgcatggg
    16201 ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat gctgaccttg
    16261 tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg aaaagcgcac
    16321 tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg gtattattcc
    16381 aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga tttatgctgg
    16441 agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg
    16501 agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact atatttgcta
    16561 cttttagttg tttgtctgtt catcgtggca tatatcccgc gcttgttccc gatgctttat
    16621 tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt accggtggca
    16681 ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct ggatattgct
    16741 tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa aacacgttcg
    16801 atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat gctttaaact
    16861 gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat ctatccgaac
    16921 tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa aagattagtg
    16981 ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt gtccttctat
    17041 gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga gggaatttaa
    17101 atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct tctatgtaaa
    17161 gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa tcaagatggt
    17221 atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt cctgatgctt
    17281 ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac aaaagaagga
    17341 gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt tgtcatcggt
    17401 atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat cagtccgatt
    17461 gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt gattgctatc
    17521 ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg gccttatgga
    17581 acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg tacttattac
    17641 ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac agtcgcttct
    17701 ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag aaaagacttc
    17761 ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa ccgatctgcc
    17821 tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt cattactatc
    17881 gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg tccagagatc
    17941 gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa taaattgaca
    18001 ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat attaagcacg
    18061 atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt ttcgattgcg
    18121 ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc actatacaca
    18181 ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt gctagcttct
    18241 ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa tcaccatcgt
    18301 tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca agtaatcgga
    18361 ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt cggtattgtc
    18421 tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc
    18481 agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt atgttttgcc
    18541 gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc aatgatttat
    18601 tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt cggaaaaaat
    18661 gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata ggttaactaa
    18721 tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt cgtttttcta
    18781 ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag gtctggaaaa
    18841 attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga tagttcggca
    18901 tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat ccctttgatt
    18961 atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata ttatggactt
    19021 ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga agttttgggt
    19081 ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca tctttcttca
    19141 gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag cggagtaacg
    19201 gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt agaagctgcc
    19261 ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat cgatcgaatc
    19321 cgagataata cctacgctta tctgattgta gatgatttga acaatttagt acgaggagga
    19381 cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc gatcctgaca
    19441 ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa agcatttgct
    19501 cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt caaactttac
    19561 gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt gcagaaaaaa
    19621 tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac acatttaggt
    19681 gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa aaaacatacc
    19741 aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt gtaaaagaac
    19801 gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct cacttcaatc
    19861 ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact tatttactaa
    19921 aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag
    19981 taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat acggatgcaa
    20041 aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca
    20101 aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac
    20161 gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa gatgcttatc
    20221 gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga
    20281 accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt
    20341 attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac
    20401 gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga agagaagcat
    20461 ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg
    20521 gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa
    20581 agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg
    20641 gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa
    20701 agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt
    20761 agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact
    20821 tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga
    20881 aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag
    20941 ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag agattcaaga
    21001 gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa
    21061 tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat
    21121 cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat
    21181 gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc
    21241 gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt
    21301 ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta
    21361 ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt
    21421 gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag
    21481 tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc
    21541 tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga
    21601 aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa
    21661 tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca
    21721 aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga
    21781 ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact
    21841 gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt
    21901 tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat
    21961 gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga
    22021 gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg
    22081 tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc
    22141 tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa
    22201 agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg
    22261 agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga
    22321 agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg
    22381 tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa
    22441 actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa
    22501 agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt
    22561 ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt
    22621 tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta
    22681 tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct tttcgtttct
    22741 agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta
    22801 aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa
    22861 atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc
    22921 cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc
    22981 tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag
    23041 agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc
    23101 aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa
    23161 tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat
    23221 ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat
    23281 gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa
    23341 agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata
    23401 gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc
    23461 acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat
    23521 ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact
    23581 gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt
    23641 gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa
    23701 cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg
    23761 gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg
    23821 gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt
    23881 tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat
    23941 gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa
    24001 gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact
    24061 tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc
    24121 aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag
    24181 aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat
    24241 cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt
    24301 attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag
    24361 tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta
    24421 gcaccttatg aagctcggtt atataaaaca aacaagtaaa acttcctcgt taaagttcat
    24481 tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt
    24541 cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc
    24601 aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca taccgattga
    24661 tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt
    24721 gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga
    24781 gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa
    24841 atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt
    24901 acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac
    24961 ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa
    25021 tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt
    25081 ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag
    25141 ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag
    25201 ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa
    25261 ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac
    25321 cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc
    25381 ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac
    25441 cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag
    25501 ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat
    25561 agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag
    25621 acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt
    25681 ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca
    25741 gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat
    25801 acctcataat gatcaattga atagagaaga tccacagctt catctccagc catccttgtg
    25861 ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc
    25921 atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta
    25981 ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga
    26041 ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt
    26101 tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca
    26161 attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat
    26221 tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc
    26281 aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct
    26341 ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc
    26401 atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat
    26461 ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta
    26521 aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt
    26581 gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt
    26641 tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata
    26701 atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac
    26761 gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact
    26821 tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc
    26881 caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc
    26941 cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact
    27001 gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca
    27061 cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg
    27121 ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt
    27181 gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct
    27241 ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca
    27301 attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc
    27361 atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact
    27421 ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc
    27481 catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt
    27541 gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca
    27601 gacaaagcag gttgttccat taaataaaga atagg
//
LOCUS       DACBZN010000028        26832 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.028, whole genome shotgun sequence.
ACCESSION   DACBZN010000028 DACBZN010000000
VERSION     DACBZN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.028"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(717..3866)
                     /locus_tag="IVD64_001188"
     CDS             complement(717..3866)
                     /locus_tag="IVD64_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6148450.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5124)
                     /locus_tag="IVD64_001189"
     CDS             complement(3880..5124)
                     /locus_tag="IVD64_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP6148451.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5117..6709)
                     /locus_tag="IVD64_001190"
     CDS             complement(5117..6709)
                     /locus_tag="IVD64_001190"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6148452.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(7218..7943)
                     /locus_tag="IVD64_001191"
     CDS             complement(7218..7943)
                     /locus_tag="IVD64_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148453.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(8097..8423)
                     /locus_tag="IVD64_001192"
     CDS             complement(8097..8423)
                     /locus_tag="IVD64_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148454.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8460..8825)
                     /locus_tag="IVD64_001193"
     CDS             complement(8460..8825)
                     /locus_tag="IVD64_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP6148455.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8862..9077)
                     /locus_tag="IVD64_001194"
     CDS             complement(8862..9077)
                     /locus_tag="IVD64_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6148456.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9206..9541
                     /locus_tag="IVD64_001195"
     CDS             9206..9541
                     /locus_tag="IVD64_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6148457.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9617..11614)
                     /locus_tag="IVD64_001196"
     CDS             complement(9617..11614)
                     /locus_tag="IVD64_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148458.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11712..11987)
                     /locus_tag="IVD64_001197"
     CDS             complement(11712..11987)
                     /locus_tag="IVD64_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6148459.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            12219..12953
                     /locus_tag="IVD64_001198"
     CDS             12219..12953
                     /locus_tag="IVD64_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6148460.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12992..14134
                     /locus_tag="IVD64_001199"
     CDS             12992..14134
                     /locus_tag="IVD64_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6148461.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14229..15620)
                     /gene="sufB"
                     /locus_tag="IVD64_001200"
     CDS             complement(14229..15620)
                     /gene="sufB"
                     /locus_tag="IVD64_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP6148462.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15625..16095)
                     /locus_tag="IVD64_001201"
     CDS             complement(15625..16095)
                     /locus_tag="IVD64_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP6148463.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(16082..17317)
                     /locus_tag="IVD64_001202"
     CDS             complement(16082..17317)
                     /locus_tag="IVD64_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6148464.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(17314..18600)
                     /gene="sufD"
                     /locus_tag="IVD64_001203"
     CDS             complement(17314..18600)
                     /gene="sufD"
                     /locus_tag="IVD64_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP6148465.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18613..19386)
                     /gene="sufC"
                     /locus_tag="IVD64_001204"
     CDS             complement(18613..19386)
                     /gene="sufC"
                     /locus_tag="IVD64_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP6148466.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19583..20431)
                     /locus_tag="IVD64_001205"
     CDS             complement(19583..20431)
                     /locus_tag="IVD64_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6148467.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(20455..21141)
                     /locus_tag="IVD64_001206"
     CDS             complement(20455..21141)
                     /locus_tag="IVD64_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148468.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(21134..22168)
                     /locus_tag="IVD64_001207"
     CDS             complement(21134..22168)
                     /locus_tag="IVD64_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6148469.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(22563..22901)
                     /locus_tag="IVD64_001208"
     CDS             complement(22563..22901)
                     /locus_tag="IVD64_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP6148470.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22903..23259)
                     /locus_tag="IVD64_001209"
     CDS             complement(22903..23259)
                     /locus_tag="IVD64_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP6148471.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(23265..24452)
                     /locus_tag="IVD64_001210"
     CDS             complement(23265..24452)
                     /locus_tag="IVD64_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6148472.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24643..25488)
                     /locus_tag="IVD64_001211"
     CDS             complement(24643..25488)
                     /locus_tag="IVD64_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6148473.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25722..26687
                     /locus_tag="IVD64_001212"
     CDS             25722..26687
                     /locus_tag="IVD64_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP6148474.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7689 a         5548 c         4514 g         9081 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtattata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccctccc taaacaaaca tcttttgtaa
     3901 gaagcctttt ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatcgtgtc
     3961 gtcgagttgt ttgaagaaag cgccgatttt ttgttgttcc tctttattcc tagtaaaagg
     4021 aaacttgata ttagcgaaat tctcatagaa taagtattct cgtacgcttc cttcagtgtt
     4081 gcgcttgacc tctttgataa atgttggtga cttgataaac tgaagtatga actcattatc
     4141 taagtcctcg ctcattttca caactacata aagagagcta acaatgaccg tcataccaag
     4201 attgttaaat gcaattgatc ctacgtttac tcttgctgga ttataggcga attcatttgg
     4261 ttcaacgaat ttataatttg ttttttctaa atcatccaac ctactaccat caaactgttc
     4321 tgtttgagaa attaaacctg ctttattact taccgaatag gcagggaagt ctaaatcttt
     4381 cgtgttcttt tttccagtct tcttactaaa ctctttgaac ttacgctgtt cccaatcttc
     4441 cgtaaatcca ggaaaccgaa tttctggcac ttttgctccg tttttaggga acattttttg
     4501 taagaatcct tttttcgttt cctttaataa atctagctta cgttgttgaa gagcgatagt
     4561 gtcgtccagt tgtttgaaga agttcccaat tttcttttgt tcttctaata ttggaaattg
     4621 tactatccat tttttcactt ctgacattgg tacgagattt gtaattgctg aacctggatt
     4681 agctcctgtg agttttcttt gcataaaatc tgcacgaata aattgaataa aatattctga
     4741 atctgtgatt tctttattta ttctaagtct aatcaaagcc tgattgaata cacccggttt
     4801 tatttgcttt ggtactcttg aaattctccc aattgttcca gcgccactca tgataaaatc
     4861 gcctggttct aatttaaatt tgattaattc attatatttt ttttcagtaa ttttatatct
     4921 tgtttcgtaa ttatcataaa tagcattttg ttgttcatag acaacaaaat tgctattaga
     4981 tacaaagaat tctttcttta aggcactccc aaatggtccc ctccttactc catctttttt
     5041 atcaagtaag cattcaaact tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat
     5101 ttctggttgt atatcattac tcatgtttga acacctccag cgcaccttgt aaccacgctt
     5161 cattttcagg tgttgtttgc agggaagaga tagcttctaa aagttctttt tctagcactt
     5221 gctgttcttg tcgtatcttc ttgatgtcat ttccgacatg aaccatatca attggttctt
     5281 cttcttcaaa cgtatctaca tagcgtggaa tatttaagtt atagtcattt tctttgattt
     5341 catcaaagtt tgccaaatga gcgtatttct cgacatcttt tctttcagca taggtttcga
     5401 ggattttttg aatgttttct tccgataatt tattctgatt ttttcctttg acaaattctc
     5461 gactagcgtc aatgaataat acgtctcgag tttggcggtt ctttttcaat acaatcaccg
     5521 tcgttggaat cgatgtcccg aagaacaagt tcgcaggcat accgatcaca gcataaatac
     5581 tgccgtcttc tagtaatttt tgacgaatga ccccttctgc agcgccacgg aataaaactc
     5641 cgtgtggcaa gacgatggcc atagtccctg tttccttcaa atgatagaaa ccatgaagaa
     5701 gaaacgcaaa atctgctttg gattttggcg ctaattttcc ataacgatta aaccgagaat
     5761 cgtctaagaa ggtcgtatct gctgaccaat tagccgaata aggtgggttc attactactg
     5821 catcaaatgt gtaaggttca tctgttggcc aatccttatt caacgtatcc ccattgcgga
     5881 gattcatttc ttcggcatct accccatgta aaatcaagtt catttttgct agattgtatg
     5941 tcgttgtatt taattcttgc ccatgatatt taacgttatc tgggtgagtt aagtagttac
     6001 gcacatttaa catcaatgaa cctgaaccca tggttggatc aaaaacacta aagaatcgtc
     6061 gttctttttg atccaacgtc acgatttgtg ccatcatatc agaaaccata tgaggcgtgt
     6121 aaaattctcc ggctttcttc cctgcttctg aagcaaattg actgatcaga aattcataag
     6181 catccccgat cacgtcccca tcgtgaccta aaacttccac gtcattcaat ttcttaatga
     6241 cttcggtaat cgtcacattt cgttgttgct catctgttcc tagttttttt gattggagat
     6301 ccacatcatc aaataaccca ttaaattgat tgtaggtaga tgccaattgc acaaaggctt
     6361 tgtttagatc attcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa
     6421 gatactctgg cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc agatcttctt
     6481 ttgattcttc atctgaaagt aattcgcgat acaacatcgt ttgtttgctt accgtatcat
     6541 attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat
     6601 atttatagaa aatcagtccc aataaataat ttttatattc tgacgcatcc attttgctac
     6661 ggagattatc tgccgcacta aataattttt gatttaattc tgcacccaaa aagatacact
     6721 cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactcc cttttagaaa
     6781 aagcaaaaaa aaaagaaaga aacttattct tgctaataaa ctacgcgaaa ggagtctaac
     6841 gttgtttgat ataacttaga ctgatcgtta acttattttt tatatttcta catagttatc
     6901 tttagaacca tttcagatac atatgaaaca attacatatg attttttcat aaaaaaacag
     6961 tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta aaatcactac
     7021 ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc gtttcatttc
     7081 agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca gtagcttatc
     7141 ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt aaataaatga
     7201 aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta atcagttacc
     7261 tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa ccgaagtgga
     7321 atatctttac caactttctc tgtcactgca ttactgatac ctgttagatt agctaataaa
     7381 tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt gattccttta
     7441 tcttcattta caagtttcca agtaggtggt atagctgtgt atgtttctac aaatgaatta
     7501 agtttttcat taagtgactt ctcttttata tttccatcta aaaactctgt atatatggct
     7561 tgagctatac taaaacaaag ctggccttgt tctaataatt ttacatcatt agttagtaaa
     7621 gctgtgttca cgttatcaat cgcttcaaga agagtatctt tggtgtccct aacagcttta
     7681 ggtgtgcctt ctggaatttc cctacttgat acctcgtcca ttttatcttc cataaacccc
     7741 ttaaaatcat atctttctcc caaatccata gaagtttttg agaaagcttt catttctgag
     7801 tctatttctt caataatatt taacatgtct gatacatatt gtttaggctg attcgttttt
     7861 aatctatatg tggtaaatat ctcaattcct ccaattatca ataaaattgc caaacaaatc
     7921 cataataatt ttttcttttt catttttcct cctatacact gctgttataa cagtgactaa
     7981 tgtaaagatt ttcccagcaa tcatcagact attatttaca gggaatcaaa aaaactcctt
     8041 agtaagctta cacctttagg agttcctgtt tgaacggtat gcataataca tttaaatcaa
     8101 tttaacatta acacctcagt atatggtatc tctacatata cacggtcttt ttttggatag
     8161 tactcgacat ttatattgat agcacttcca ttgtctaacg tagctttagc ccggtacgtg
     8221 ttatcaccat tatcatgagt gatcgtcaca tcatcacatg tccgatttaa gttatactgt
     8281 tcttgtaaaa tctgagttac caatggtaca gatgacttct ctagtccaga tgaacccgtc
     8341 attcttgaaa aaattatacc tataatagcc aatacaacga tacctataat gacaaacttc
     8401 ttattcatat ttactttatt caacttttta tctcctatct ccaaaatgat tttctctcat
     8461 cagtaactca ttgacatccc caatagtaca ccaaagatgg ctccagctac ccctgctatc
     8521 atcaaaatcg tacccatttg ttcatctttc tctcgatata agtaacccat aataacagca
     8581 acagcaccaa ataccacagg aataaatgct aatgatacta ccatagaaat ccaaccaata
     8641 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
     8701 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtaat
     8761 gctccgcaaa actggcaata cttactttca tttgttattt ctttaccaca ttttctacaa
     8821 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gctaactgtt tactttatta
     8881 aatacatcag aaaacagctc atttattggt acacttaata tctcagaaag cattttcatt
     8941 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     9001 ttcatttttt tggctaactc agtttgggaa agtccttttt cttttctgta tcggctgaga
     9061 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
     9121 ttcaactcat aaaatatata aatacttcaa atgtcattac aaatacaaca acttgttgta
     9181 ctctactgag aaggggggtg tattaatgaa acagaagcaa tatgcaaagg tcattagaga
     9241 aaagcgaaaa gcactcgggt ggacacaaaa agaattagct aaaaaaatct tctctacaca
     9301 acaggcagta gcaagatggg aaaattctgt aacagaacct aacctcgata gcttaacagc
     9361 tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg acatagatta
     9421 tgaggaagag tttttggctc tctatcgctc tcttagtact gaagatgcaa ttcgaacaat
     9481 tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg
     9541 aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga
     9601 gctatggata acctgtctag tttaatataa ttgggtctct tctattcctt actctccgtc
     9661 catcatctct tgttacactt gtaaattgat aaataggaac taattcaact cctgataata
     9721 tcaattcttt tattacttca tctatccctg cccctttcgc tagatatact aaaaaactac
     9781 tgcttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca agagtgaatc
     9841 cttgtttaac aaagtagcct ctgttttcta ttaaagcatt tttcacgctg ttatccctga
     9901 catttcgtag gcgttcacaa acagtgttaa taagttcttt ttctgtcatt agatatgtcc
     9961 cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct
    10021 tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac
    10081 atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg
    10141 gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat
    10201 agacattgct tctcttatgt ttaaagtttc ctaacgcttt ctcttccttt aacctattta
    10261 ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa
    10321 aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaag
    10381 aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt tccttactat
    10441 catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat
    10501 aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt
    10561 ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc
    10621 aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat
    10681 tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg
    10741 taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg
    10801 acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca
    10861 tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat
    10921 tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac
    10981 tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca
    11041 tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat
    11101 tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag
    11161 caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt
    11221 ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat
    11281 tgatagataa aaccttttct atgcttaagt atctactatc atctttactc aaatctacta
    11341 ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca
    11401 caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg
    11461 aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat
    11521 ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt tcttgatcta
    11581 cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg
    11641 ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat gttttagtct
    11701 tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat
    11761 ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc
    11821 gttcctactg atacacctag atattcactt gcctgctgat agtccatata ctctagagtg
    11881 actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt
    11941 tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct
    12001 ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa
    12061 agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca
    12121 cataatacct cgataaaaaa ggggtgtcaa cagctttgta aaatttattt acaacacccc
    12181 ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt ccaccaataa
    12241 attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa
    12301 aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaaa
    12361 ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg
    12421 ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt
    12481 tgggaattat tctttataga gatagcattg tactagatgg tataacattt ccagataaaa
    12541 acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc
    12601 tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta
    12661 ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca
    12721 tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag
    12781 aactactaga aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc
    12841 ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat
    12901 caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag
    12961 tactccttgt aaactaagag gagatgattt aatggcaaaa atatcaaagt acaaaaagaa
    13021 aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga
    13081 aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga
    13141 cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga
    13201 agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc
    13261 aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc
    13321 gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag
    13381 ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata
    13441 tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa
    13501 tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg
    13561 taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga
    13621 agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa
    13681 aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc
    13741 tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca
    13801 gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt
    13861 tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat
    13921 tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag
    13981 tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa
    14041 tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg
    14101 tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt
    14161 ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta
    14221 taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt gagttcgact
    14281 gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac gatcatttca
    14341 gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc ttctgagatt
    14401 tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc attgaatggg
    14461 atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat atgagagatc
    14521 gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc tccgccgtct
    14581 tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg gatcattttt
    14641 gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat ggtgcctcgt
    14701 gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc acctaggttt
    14761 ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt tactaagttg
    14821 tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc ttttcttgta
    14881 aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc cgtacaacct
    14941 tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc gaattgcccc
    15001 gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt gacgcctttt
    15061 ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc taatttgttg
    15121 tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta ttctttcaaa
    15181 gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt catattatgg
    15241 tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg ttcagcttct
    15301 ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc ccagtcacga
    15361 gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat gtctgaaagg
    15421 tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt cagacggaat
    15481 tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac ttcttctgtc
    15541 aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa tttatattct
    15601 tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat ggaaagctgc
    15661 ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg cacatttgat
    15721 ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt ggtcgggatc
    15781 tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt ctgcttcttc
    15841 tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac tgatggagca
    15901 gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga cttgcaattc
    15961 gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt cagtcaagct
    16021 tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata aattgtctaa
    16081 tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca actaaacggt
    16141 cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct gatacttcaa
    16201 ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc ccttccatgt
    16261 ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca acgactcctg
    16321 tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct tgtaattttg
    16381 gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata ccgagttttt
    16441 gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt gtccctgctt
    16501 cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa tcgatcattt
    16561 cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca tataaaacac
    16621 caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca cagtctagtg
    16681 cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg atgaccccgc
    16741 cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct aagacattgg
    16801 atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct gcatcctcca
    16861 tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct gttcgttttg
    16921 ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc actatctcgt
    16981 ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag cttgtcgtcg
    17041 taccacgagt aaatagcact tctgctgttt cttttgcatg gatgaacgcc cgtacttttt
    17101 ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca ccgcgatgaa
    17161 cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt tttggctttt
    17221 gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct tgaaataaga
    17281 taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc cttcgattac
    17341 ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag aaccgaggaa
    17401 tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca tcagatagta
    17461 catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta cttcgttttc
    17521 atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca tcaagacacg
    17581 actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga taccattaaa
    17641 ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga tcgtatgagg
    17701 ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc tgattgccac
    17761 gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt cagcgatcac
    17821 atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta aaatgtgccc
    17881 acggcgattc atataagaag taatattttc tcctaattga tctacggctg aatatttgat
    17941 ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt ttgctacttg
    18001 ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat ctgccacgat
    18061 caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata aggactcgag
    18121 tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt tcataaaagc
    18181 tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca tataatattc
    18241 ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa tgactccttt
    18301 ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt ggacaagcac
    18361 aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga tggtttgtgt
    18421 ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat gcggcaatgg
    18481 cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat caggttcgcc
    18541 tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt tttctttcat
    18601 tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt tactgatacc
    18661 agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca tgactactcg
    18721 gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc gttgatagtg
    18781 ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt tagcaacgac
    18841 tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa atgtaggttc
    18901 taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa aaccttcgtt
    18961 cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt catctaactt
    19021 tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat tgatcgctgc
    19081 acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca tagctaaaaa
    19141 taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc cgtcaaacaa
    19201 aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag ataaggtaga
    19261 ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca ttgttaggtt
    19321 tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta tttctaagac
    19381 agacatgcat tataccccca aattctcttt cgttctcact cattttagac taattgagaa
    19441 tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat taaacaattt
    19501 tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac agacttattt
    19561 ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta acagagccgc
    19621 cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca atcagttttt
    19681 tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg taaggagagt
    19741 tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg taagcgaagt
    19801 ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct aaaagtgatg
    19861 ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca tcgaatgttg
    19921 ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt agaattgtga
    19981 tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca tcttttaatt
    20041 cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg acagcaccgg
    20101 cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga atatgctgga
    20161 agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca tagtcatcga
    20221 atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca tgttccaaaa
    20281 tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct gctttatttg
    20341 ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc agcaaaagcg
    20401 ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat cctattatct
    20461 tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata cgattagtaa
    20521 aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc cttcctgcca
    20581 agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa atccgatcat
    20641 cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag gtatcaagac
    20701 cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca gtacaccagg
    20761 gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg cggataaaac
    20821 aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt tagtaaatgg
    20881 aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac tgatgatcga
    20941 tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag agtaaaggat
    21001 caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg tcatatatag
    21061 tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat ctagatatgt
    21121 ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt tctttgatga
    21181 acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc aatgaaccaa
    21241 aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg atgttatagc
    21301 gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag gtcaaagttg
    21361 ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc gaatcagctt
    21421 ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt tgtctgaaga
    21481 cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg actttgttgc
    21541 agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa ttcaatttct
    21601 tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc aatgcgctag
    21661 tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg ataccgacgc
    21721 gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct tctaacccga
    21781 ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg ccagctaatt
    21841 ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg aaatgctgga
    21901 agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat tttgtaatat
    21961 cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc aggagacgga
    22021 ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc cctttttcaa
    22081 cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg aatgtctttt
    22141 tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat gatttttcaa
    22201 acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa cttccgtgtt
    22261 accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg gacttagctt
    22321 ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct tacgctcgct
    22381 tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag caaacagggc
    22441 tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt tcatatagga
    22501 ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt gattttctga
    22561 ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat ccacgcattc
    22621 atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt aacagaagaa
    22681 actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc aacttcgatc
    22741 agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa cgtgatgtct
    22801 ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc attaaataag
    22861 atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca gttctccttc
    22921 ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga tcagtggtcg
    22981 cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg taaactcgcc
    23041 tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga acaggcgacg
    23101 gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg gtacttcttt
    23161 gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt tggctttttt
    23221 gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct ccttgaagat
    23281 cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc gatcatgttc
    23341 cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt taaaggaagt
    23401 aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat gatcaaaccg
    23461 cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat catacgatag
    23521 attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa gttttcaccg
    23581 ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct tacgttgaat
    23641 cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg ctgataactc
    23701 attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa ctgataagat
    23761 ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa gaagatcgtt
    23821 cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca agagattcca
    23881 gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc aaagtcatct
    23941 tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc aatcagccaa
    24001 aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac gatcagtgcc
    24061 atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa cgcaccgaat
    24121 cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa tttgagcaag
    24181 agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca gatccatttg
    24241 gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca gacaacttgc
    24301 atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc tacatataat
    24361 gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata atcaatacga
    24421 ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta aaaaccatac
    24481 tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga catttgggaa
    24541 aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc ctactgctta
    24601 tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc tgagattctc
    24661 ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg caatctctag
    24721 cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat ggaaattcaa
    24781 cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc gaccgattcc
    24841 ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct tatcttggat
    24901 attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg cttctcttac
    24961 aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca tcaaagagta
    25021 tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg attcatgttt
    25081 cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat gcactttagg
    25141 aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa aggtgtggga
    25201 agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt gctgagtttc
    25261 ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt gcttagttgg
    25321 aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc gctccaactg
    25381 tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg ctctggaaaa
    25441 atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg caccacttcc
    25501 ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa aaaaccatct
    25561 ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga tgaagacaat
    25621 cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta ttcgttcatc
    25681 ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac tttttgaaaa
    25741 ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga aatctgttta
    25801 aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg attcttatga
    25861 tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg ttgcatgatc
    25921 caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt tgtacttatt
    25981 ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc gctgttatct
    26041 tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct gatctttcaa
    26101 ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc atcactgtaa
    26161 ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca ttgatctgct
    26221 atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat atggctcatc
    26281 cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta acaggttatc
    26341 ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct ttatctcaag
    26401 tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt ttttatccaa
    26461 gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt actactgttc
    26521 attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa gttgcggaat
    26581 gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt ttcttaatta
    26641 aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa tcgattcaat
    26701 aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa aacagctgtt
    26761 catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac accgtttacc
    26821 aagaggtcgt aa
//
LOCUS       DACBZN010000029        26475 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.029, whole genome shotgun sequence.
ACCESSION   DACBZN010000029 DACBZN010000000
VERSION     DACBZN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.029"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(98..1228)
                     /locus_tag="IVD64_001213"
     CDS             complement(98..1228)
                     /locus_tag="IVD64_001213"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP6148475.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IVD64_001214"
     CDS             complement(1877..3079)
                     /locus_tag="IVD64_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6148476.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IVD64_001215"
     CDS             complement(3139..3342)
                     /locus_tag="IVD64_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6148477.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IVD64_001216"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IVD64_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IVD64_001217"
     CDS             4879..5691
                     /locus_tag="IVD64_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6148478.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6912)
                     /locus_tag="IVD64_001218"
     CDS             complement(6715..6912)
                     /locus_tag="IVD64_001218"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148479.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IVD64_001219"
     CDS             complement(7058..7936)
                     /locus_tag="IVD64_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6148480.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IVD64_001220"
     CDS             complement(7975..9093)
                     /locus_tag="IVD64_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP6148481.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IVD64_001221"
     CDS             complement(9090..11189)
                     /locus_tag="IVD64_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP6148482.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IVD64_001222"
     CDS             complement(11240..12061)
                     /locus_tag="IVD64_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6148483.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IVD64_001223"
     CDS             complement(12058..12867)
                     /locus_tag="IVD64_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6148484.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IVD64_001224"
     CDS             complement(12888..13382)
                     /locus_tag="IVD64_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6148485.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IVD64_001225"
     CDS             complement(13394..13882)
                     /locus_tag="IVD64_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6148486.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IVD64_001226"
     CDS             complement(13959..16655)
                     /locus_tag="IVD64_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6148487.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IVD64_001227"
     CDS             complement(17083..18156)
                     /locus_tag="IVD64_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6148488.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IVD64_001228"
     CDS             complement(18153..18980)
                     /locus_tag="IVD64_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148489.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IVD64_001229"
     CDS             complement(18981..19787)
                     /locus_tag="IVD64_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148490.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IVD64_001230"
     CDS             complement(19799..20884)
                     /locus_tag="IVD64_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148491.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IVD64_001231"
     CDS             complement(20901..21443)
                     /locus_tag="IVD64_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6148492.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IVD64_001232"
     CDS             21701..22690
                     /locus_tag="IVD64_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP6148493.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IVD64_001233"
     CDS             22687..23424
                     /locus_tag="IVD64_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP6148494.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IVD64_001234"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IVD64_001234"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP6148495.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IVD64_001235"
     CDS             23988..24620
                     /locus_tag="IVD64_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6148496.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IVD64_001236"
     CDS             24705..25352
                     /locus_tag="IVD64_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6148497.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IVD64_001237"
                     /pseudo
     CDS             25357..26340
                     /locus_tag="IVD64_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
BASE COUNT         8101 a         5593 c         4487 g         8294 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagctgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactagc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgt
//
LOCUS       DACBZN010000030        25748 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.030, whole genome shotgun sequence.
ACCESSION   DACBZN010000030 DACBZN010000000
VERSION     DACBZN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.030"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(70..789)
                     /locus_tag="IVD64_001238"
     CDS             complement(70..789)
                     /locus_tag="IVD64_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6148498.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(920..2071)
                     /locus_tag="IVD64_001239"
     CDS             complement(920..2071)
                     /locus_tag="IVD64_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP6148499.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2288..3028)
                     /locus_tag="IVD64_001240"
     CDS             complement(2288..3028)
                     /locus_tag="IVD64_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6148500.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3032..3355)
                     /gene="rsfS"
                     /locus_tag="IVD64_001241"
     CDS             complement(3032..3355)
                     /gene="rsfS"
                     /locus_tag="IVD64_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP6148501.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3412..4005)
                     /locus_tag="IVD64_001242"
     CDS             complement(3412..4005)
                     /locus_tag="IVD64_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6148502.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3989..4636)
                     /locus_tag="IVD64_001243"
     CDS             complement(3989..4636)
                     /locus_tag="IVD64_001243"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP6148503.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4649..4960)
                     /gene="yhbY"
                     /locus_tag="IVD64_001244"
     CDS             complement(4649..4960)
                     /gene="yhbY"
                     /locus_tag="IVD64_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP6148504.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4976..6085)
                     /gene="yqeH"
                     /locus_tag="IVD64_001245"
     CDS             complement(4976..6085)
                     /gene="yqeH"
                     /locus_tag="IVD64_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP6148505.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6082..6609)
                     /locus_tag="IVD64_001246"
     CDS             complement(6082..6609)
                     /locus_tag="IVD64_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP6148506.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6789..7574)
                     /locus_tag="IVD64_001247"
     CDS             complement(6789..7574)
                     /locus_tag="IVD64_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP6148507.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7567..8436)
                     /locus_tag="IVD64_001248"
     CDS             complement(7567..8436)
                     /locus_tag="IVD64_001248"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP6148508.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8437..9816)
                     /locus_tag="IVD64_001249"
     CDS             complement(8437..9816)
                     /locus_tag="IVD64_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP6148509.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9824..10243)
                     /gene="fabZ"
                     /locus_tag="IVD64_001250"
     CDS             complement(9824..10243)
                     /gene="fabZ"
                     /locus_tag="IVD64_001250"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6148510.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10268..10744)
                     /gene="accB"
                     /locus_tag="IVD64_001251"
     CDS             complement(10268..10744)
                     /gene="accB"
                     /locus_tag="IVD64_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP6148511.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10747..11982)
                     /gene="fabF"
                     /locus_tag="IVD64_001252"
     CDS             complement(10747..11982)
                     /gene="fabF"
                     /locus_tag="IVD64_001252"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP6148512.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12001..12738)
                     /gene="fabG"
                     /locus_tag="IVD64_001253"
     CDS             complement(12001..12738)
                     /gene="fabG"
                     /locus_tag="IVD64_001253"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP6148513.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12750..13667)
                     /gene="fabD"
                     /locus_tag="IVD64_001254"
     CDS             complement(12750..13667)
                     /gene="fabD"
                     /locus_tag="IVD64_001254"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6148514.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13774..13998)
                     /locus_tag="IVD64_001255"
     CDS             complement(13774..13998)
                     /locus_tag="IVD64_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6148515.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14051..15013)
                     /locus_tag="IVD64_001256"
     CDS             complement(14051..15013)
                     /locus_tag="IVD64_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP6148516.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15023..15463)
                     /locus_tag="IVD64_001257"
     CDS             complement(15023..15463)
                     /locus_tag="IVD64_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6148517.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15769..16734
                     /locus_tag="IVD64_001258"
     CDS             15769..16734
                     /locus_tag="IVD64_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6148518.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16791..17021)
                     /locus_tag="IVD64_001259"
     CDS             complement(16791..17021)
                     /locus_tag="IVD64_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP6148519.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17107..17994)
                     /locus_tag="IVD64_001260"
     CDS             complement(17107..17994)
                     /locus_tag="IVD64_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP6148520.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18083..19306)
                     /locus_tag="IVD64_001261"
     CDS             complement(18083..19306)
                     /locus_tag="IVD64_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6148521.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19323..20402)
                     /locus_tag="IVD64_001262"
     CDS             complement(19323..20402)
                     /locus_tag="IVD64_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6148522.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(20533..22260)
                     /gene="ptsP"
                     /locus_tag="IVD64_001263"
     CDS             complement(20533..22260)
                     /gene="ptsP"
                     /locus_tag="IVD64_001263"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP6148523.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(22260..22526)
                     /locus_tag="IVD64_001264"
     CDS             complement(22260..22526)
                     /locus_tag="IVD64_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP6148524.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23005..25239
                     /locus_tag="IVD64_001265"
     CDS             23005..25239
                     /locus_tag="IVD64_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6148525.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQKAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            25387..25707
                     /locus_tag="IVD64_001266"
     CDS             25387..25707
                     /locus_tag="IVD64_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6148526.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         7163 a         5603 c         4466 g         8516 t
ORIGIN      
        1 agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa
       61 actggttgct tagtcttcta atgccacatt atggaacaca tgctgtacat cttctagatc
      121 ttccagtgca tcaatcattt tttcaaatgt ttcttgatct tctggagata gtgttacctc
      181 gttttgaggg atcatttgga tctcagcgat tgaaaaatca gtgattcctt tttcttttaa
      241 tgcttcttgt atactatgga aatccgctac ttcaccgtaa acgatgattt gtccttcttc
      301 ttccattaca tcgcgaacat cgatgtcttt ttccattagg tattcaagga tctcgtcagc
      361 gtcttctcct gcaaaagcaa agattgctgt attgtcaaac atgtaagaaa ccgctccgga
      421 aacacccatg ttcccgccat ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt
      481 cacattgttc gtcaatgtat caacaatcac cattgagccg cttggtccaa atccttcata
      541 gcgcaattca gagtatgttt catctcctgc accttttgct ttttcgattg ctcggtcaat
      601 aatatgtttt ggtacattat aagttttagc gcgatcaatg acgaaacgta gtttttggtt
      661 tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag atttcaatcc caaattttgc
      721 ataaatccgg ctattattag catctttagc agttttcttt gctacgatat ttgcccattt
      781 acgtcccata ttttcacttc gctttctgat ttgatagttt cataacgcaa aaacgtcctg
      841 ttccattata cttgtgaatc acgattctgt gaagcttttt ttctttcttc cttcattctt
      901 ctcattttta gtcaattatc tatactcgga ttgggatttg cccgaaattc tgttccggaa
      961 tattgcctcc caattgatag atttgatcac ttctaactaa cagttccgct tcttgtgcct
     1021 ctttttttcc gattcgggaa taaatagaaa tctctttttt ttcttttaag aatcttcttc
     1081 cagcatcggt aaatccaagt agtcgaagat aatttttatt ttgttggaca ggaatctctt
     1141 gctttttgat attcagcaag acataacaag ataatcgttg gatccgtgtc catgtatatc
     1201 tttttgtttt tacctttgac acaaaatcct caaaactatc agcagttgca gctgctgttt
     1261 tcatgcgcga ttctattcct tcggtcatct gatagatatc tgtcagttcc agcaaagtag
     1321 aagataacaa ccgataacgc aaatacggcc aataagattc ccaagtctgc agcgaacaag
     1381 ttgaaagatc ttcttctgtc tgaggcggca caagtccagc tattcttttt ccttcttcta
     1441 acgcatggcg aatggctgtt gcactggcta tcctctgatc tgtgatgatt ttttcatgat
     1501 agcctgcact tttacgtcgg atcggatgta attccatagg gtggacatac ttggcatttt
     1561 cctttgcata acttaatccc aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg
     1621 gatacagact gcgaaaaacc tcagtcattt tttgcggata actatacgtt tgatcagaca
     1681 gttcttgaaa tgcctcttct atcctatctt gattctcttg gacaaagcgt ccgaaactgc
     1741 tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa actgtcacag tccagtgttt
     1801 gcaagatttt tactgcacct ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg
     1861 gaagctccac tactaaatcc acaccatttt gtaaagcttc attcgcgcga tgccatttat
     1921 cgatgatagc cggttcgcct ctttgcaaaa aattcccgct cattacagca atgacgacct
     1981 ctgctcctgt tatttttcgt gccatttcca catgatagcg atgtccattg tgaaaaggat
     2041 tgtactctac gataatcccg cagcttttca tgcgcatcct ccttcatagt tatcgataaa
     2101 aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa aaatacccga actatataaa
     2161 ctattctcgc aaacacaata cttctgcgac gaaaagctgc actgcaagag caatgcctat
     2221 atcgttcgga tatataattt atgttgaaga tttattcttt tcttgttaac tcgagcaggc
     2281 aaaaccgcta gtctttgtat gctacaaaga accagcgctt tgatttctct gatgctgctt
     2341 cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc gctgttttcc agcattctta
     2401 aataactttc caatggatag gtacgctctt gatgtagttc atcttcacga ataaatcgac
     2461 cagacccttc ttgttcttca acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc
     2521 ttcctggata actatcccac aaaaaagcaa aattatcccc ttgataatga tagctatatt
     2581 ctggaaaaac ctcatctact tgataggtgg agtgtacgtc aaataaaaat cggccatttt
     2641 cttctaacgc ttgatagact tcatcaaaca cttgctgtac ctcttgtcta tttttcatat
     2701 agcacagcga atcagaaaaa caggtaattg cctgatattg tccgatttct gataaatcga
     2761 gcatattgcc ttccacaaat tggatcgatg cgtcttcttc atatgctcgc tggctggcaa
     2821 tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt atatccatct tttgcaaagg
     2881 caaccgctaa tgctccagta ccgcatgcca gttccaaaat attttttgtg tcttcttgta
     2941 aatgacgttt cgaaaaagct aaccattgct gataaagtgt ttcgtccatc acttcatcgt
     3001 aaatgaaagc aaatgtttca taagccatat gttaatccaa ccattcacta agatcgacta
     3061 gcggagcatc tgaccatagt ttttccagat tatagaaatt tcgttctggc gcatggaaaa
     3121 catgtacgat cacatctcca agatcgatca atacccattt tccgccttct tttccttcaa
     3181 tacgttttac ttcatatttg ttttcttctt ctttatcaat gatttcttct gtaattgcgt
     3241 tgatttgacg ttcgctattt gctgaacaga tcatgaaata atctgccaac aaggaaacat
     3301 ttcgaacgtc taaagcgata atgtcttctg cccgttttga atctgctgct tcaatcgcaa
     3361 tttttaattg ttctttacta tctatgatga tttcctccta atatgagcag cttatttttt
     3421 tgctacccag taattatatg tttcgattgt ttttggataa atctgttgtt cttgcgcaag
     3481 caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct tcgtctagat cagcccatgc
     3541 aatggctcgt gcatcttgta ccccaggaaa atctcgacct ggttcaatat agtctgctac
     3601 ataaatgatt ttgtcaagga gactcatctt agcagcccca gtcgtatgca cccggattgc
     3661 atgaagaatc tctgcatcgg tgattcctaa ttcccgctct acaaacgaag cgccaacaag
     3721 accatgccag attgcattat tataattcaa taactccgga tcaaaaccgt ctcttacgat
     3781 taccattttg aactcctcat ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat
     3841 actagctttt tcaggagaag caccgtattt tttggcaaga gccacggctg tttcttctac
     3901 tccaagcaca tgcttgaagc gttgttcgct cattctcatc tgtatttttt gcatcaggtt
     3961 ttctctagaa taagcagtat attcattact atagtccata ttcatacaac ccttcatttt
     4021 gaatataatc aattacttta tctggaacaa ggtagcggat agaacagcct tctttgatct
     4081 tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac atctacccaa attacaggat
     4141 atggagtatc cgttgtgtat cctggtcgac gtatacccac aaagttgacc atagaagtca
     4201 gctcatcgat tttataccat tttggcaaat attctaccat atccccaccg ataataaagt
     4261 aataatctgt atccggattg ttttgcgtca attccttcat agtatcatag gtgtaacttt
     4321 tcccaccacg agctaattca atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta
     4381 attccaacat gttgagtcga tgttttgcat caatcgtctg tttctcatca acatgaggtg
     4441 gctgataaga aggcatcaaa aatacgcggt caagacctaa attccggcca gcttgttcag
     4501 ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc gcccaagatc cctacttgct
     4561 ttttcttctc taagaattga ggcatttctt caggaaataa ttgtgaacga acaaatgtct
     4621 tagcttgcgt attcatcatg gtcactcctt atattgcttt tacttctttt gatagccttt
     4681 gatacttttc ttttgtggaa ggtttgtata atacaataac acgtccaatg atttggacga
     4741 tttcgcaatc tatttctttt tctaatactt gagctgcttc ttctgcaatc tcatctgtat
     4801 tttgaagtaa cgatactttg atcagttctc ttttttctaa agcttcttca atttgaacga
     4861 tcattgctga attgatccca ccttttccga tttgaaaaat cggctgaaga tgatgcgctt
     4921 tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat aattttcctt cttccttaaa
     4981 tcaatgcttt gcgtttcaat acatctacac cctttggcgc ccagcccgca attactactg
     5041 gcgctgttac agtgatccag cctagaccgg caaaaacgat gtctgttttt tctttcacag
     5101 aaaactcaaa acgaacaagc tccggaaatg cctcgacttc atcaggacga ggaggctgta
     5161 ataatccacc tacatgtttt tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa
     5221 tatttacatc atttgacaca taagcaataa atgaagcacg attgccgctg ataaaatcaa
     5281 atcttgcaag tccgcccaag aaaagtgttt gcccttcatt cagctgataa actttaggtt
     5341 tgatttcttt taacggtgca atgatccgca aatctttttt acctaagtag tgcgccattt
     5401 gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct
     5461 cgattttatc caaggtcgtt cctggaaact gcgaagttgt gattacatct tgtactcccg
     5521 ctgtattttt gatgatttga ttgataagcg tcgattttcc gacatttgtt actccgacga
     5581 tatagacatc ccgtccatcg cggtattctt cgattttttc taatagttcc gtcatctcat
     5641 gagatttctt agcactagtt aataatacat ctactggacg aattcctgct tcgtaagcac
     5701 gttccttcat ccattgtgtt aatttagttt ttttcaatga tttaggtaat atatccactt
     5761 tgttcccaac taaaagtact ggattgtcac cgacaaaacg gtgtagtcct gggattagtg
     5821 aaccattgaa gtcaaaaata tccaccacat tgacgatcaa tgcatctttt tgccctaatt
     5881 cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac atcttgggtc tcattataat
     5941 ggcgtaatct gaaacaacgc tgacaataga cttccccgga agctaaacct ttttccaaag
     6001 ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt ttgtataacg gcaccacagc
     6061 cgatacattg taattcttct gtcattcgat tccacctcgc cagcccatat cagggtgttt
     6121 tgctaataga taggccataa ttcttttttc ccaaaaacga ttaaattgtg tcttccaact
     6181 atctgttgcg ataatgggtt ttacaaggat gctacgtatc cctgcacgat tggctccacg
     6241 tatatcagtc ataatctgat cgcctaccat tactatctca tcatctgcta aatccaattg
     6301 tttctttgct atgttgatcc ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag
     6361 atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac
     6421 agggattcct gcatttttca tttctaaaat ccaagtaagc aactcttctg ttccatctgg
     6481 attattccat gcaatcaacg tattgtctaa atcggtcaat actgctttga tgcctagatt
     6541 ctttaattgg gcaggtgtaa tctgatagat ggcttctacc atccatgttg gtttgtaatt
     6601 tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag cacataaagt actcctcaac
     6661 cccgtattat actgcatatc agcaaaaaaa actacctcta tactcgttcg tctttcagaa
     6721 aaagcatttc tttacaaaat gaccaaaaat aaagattgat cacagaaaaa tttagaaaca
     6781 aaaaacaact aatattggcg gaacctcttg tatcgttttt ccagtaattt atgctgatct
     6841 aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta
     6901 tatcgtggca actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac
     6961 tcgcgtagtt ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg
     7021 tctttccaca aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc
     7081 agcatccaga cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca
     7141 atgatgatgg cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata
     7201 gcctctcctt cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc
     7261 aaggtaatca ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga
     7321 tagccttcag gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct
     7381 ttttgaatac cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc
     7441 gcttcgtcat ctgcaaataa acgatcacca tggcattcct cgaaagaatc gaagatctct
     7501 tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt
     7561 gctgtcttat ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat
     7621 tttctgtttc aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa
     7681 ttcagccttt tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct
     7741 tccagcaaaa ccgatcaagc tttggggttc tgctagaata atatcgcctt ccattgcaaa
     7801 actggcagtt actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc
     7861 attgctatgc cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc
     7921 ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt
     7981 tgctcgttcg aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat
     8041 aaaattagga tccatcactc caataatgaa gggaatcccc tgaatggttg ctttaccagt
     8101 gaggactgct tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc
     8161 gggaaatgct agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc
     8221 atcaataatc aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg
     8281 gcatactttt tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt
     8341 tgcccacatg ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt
     8401 gatccgaata tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc
     8461 tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat
     8521 gactgcagga tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt
     8581 gataaattca cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg
     8641 gacgattatt tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac
     8701 tgcactgtct actcgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc
     8761 tggagaagga gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg
     8821 tccttggaat actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac
     8881 ctgttttttt actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat
     8941 tcgagtattc atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt
     9001 tccggcattt tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc
     9061 tctttttgtt tgtgagatca ctacagaagg agattcttcc agtacttttt gattgttgcg
     9121 ttgtaaagaa caatctcgct cgcctaaatg aatgacatga ccaaatcgat cacctaagat
     9181 ctgaacttct atatggcgag caggataaat gattttttcc aaatacatat catcatttcc
     9241 aaatgccgct gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga
     9301 ttgaactttc cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata
     9361 accaattctg tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc
     9421 tggaatgact gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat
     9481 tgcatcgatc gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga
     9541 ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt
     9601 cacgattgca gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg
     9661 gccaatgcag attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga
     9721 atatatcgct acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat
     9781 ttcaccgcgg ttagctatca atactttaga aaacatattt cacctatcca atcataaagg
     9841 ttaattccgc ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg
     9901 ctgaagcttt cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt
     9961 tcttacggaa ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt
    10021 caagagaaag taatgcaact gctcctgctt gagccattgc ttcaacaatc aatacccctg
    10081 gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt
    10141 tcttagcaac gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg
    10201 gataacgatg tggaatgatt tcttttattt cttgtatgtt cattgttttc cctctttcct
    10261 gctttgctta tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt
    10321 tttctacaag gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt
    10381 tcatcgcttc aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga
    10441 aagctggttt gtcgggcgct ggttgcagat aaacgattcc cacgattggc gaaacaacct
    10501 cgattccatc aaatactgcc gattcttttt ctttcattat ttctggagca gttgttgctt
    10561 gttcaggtat gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat
    10621 tcatgcctcg acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat
    10681 cgaattccgt tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat
    10741 ccaaaattat tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa
    10801 tgaattactc attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa
    10861 gtcaatctca ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa
    10921 tgccagcaaa gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat
    10981 cgatttcgtg ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc
    11041 ttttgcttct gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac
    11101 ttgttccggc tgtacaccag cttcttccat cgcaagcttc attgctttag atgcaccaga
    11161 accatctgga gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaacgat
    11221 ttctccatag attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac
    11281 gcctgctcct tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg
    11341 attaggatca gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc
    11401 acaaatcgtt gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt
    11461 acggaatgct tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca
    11521 aatacctttt gcacctacac gcaaagcaat atttccagct gccatgttgg caatcgccat
    11581 tggtacgaac attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt
    11641 ttcgattgtt ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt
    11701 cattttttct ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata
    11761 gatcgaaaac atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa
    11821 atctttgact tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg
    11881 tgcgatgcca ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat
    11941 aggtgaagtg actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc
    12001 ttatccgtgc atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt
    12061 ttgtgctaag aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg
    12121 aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt
    12181 gatgaatcct ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac
    12241 agattttgtt aatcctacta cgccagcttt actagctgca taatttgctt gaccaatatt
    12301 tccaatcaac ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat
    12361 ttttttcaat acgtgctgcg tcatattgaa ggttccaatc aaattgatat ccagacattt
    12421 tttgaaatct tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt
    12481 tactaacaca tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt
    12541 ctcataatca gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc
    12601 ttctatcttt tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt
    12661 tgcaaaagct aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt
    12721 ttttcctttt acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc
    12781 tgcttattct caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt
    12841 aatacttttc cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg
    12901 atactctcat agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt
    12961 tctttcttca ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat
    13021 tgaatctgac ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga
    13081 aaaggcccgc tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa
    13141 acagcttcgt ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa
    13201 tttgcaggag atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca
    13261 atcggcgcat tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata
    13321 taagcgcctc tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct
    13381 actaatgccg aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc
    13441 tccttcaata agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc
    13501 gtctgatcca aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc
    13561 tcacttgctt catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata
    13621 ccttgatatt gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat
    13681 atatccagaa tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag
    13741 gaaatgtccc agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt
    13801 cactaaatct tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc
    13861 ttcaatttca ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat
    13921 gtcagtagtc aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg
    13981 aattttttcg aataccataa taatatattc ctccgatatt gtttgctttt tatttttata
    14041 atttaacagt ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg
    14101 taaatattac tttctgctgt tttcctaagg tgatctttcc actagaaact gcttcatcca
    14161 ataagatagg gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact
    14221 tttcttgcgg catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat
    14281 gcaaaagcag ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt
    14341 ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg
    14401 aatcttctga atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc
    14461 tggtaccgtc agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga
    14521 gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta
    14581 gcttagatag tgtttcacca ccaatcacca gtcccttagt tgagccgcag cggatcattt
    14641 tttcagccat gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga
    14701 tagcatgagt tgctcccaat ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat
    14761 cgggcgacat tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt
    14821 ttatcaattt ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc
    14881 ttcgttcacg aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg
    14941 aaagatcatt attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc
    15001 caaattgttc cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca
    15061 gtgcatccag caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca
    15121 tttcacgatg aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca
    15181 actttacaac gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta
    15241 acttgttaat agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg
    15301 aagttgtttt tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca
    15361 gatctttgaa ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga
    15421 cactaacaag ataatcattt actgttttta aatctggttc catgttccac cgtccactaa
    15481 ttagtttgat tatcaaatct tttgatgttc aaactatatc ttaacttttt tcaaaatgca
    15541 accttaaaag atcatttttt tactatatga gcttttttta agattttact catttaaaac
    15601 aactgataaa tcagcattta taatttcttt tattagaaaa atcatctatc tcacatattt
    15661 tgattatcaa agtattttct gtgaacagaa ctatagaatt ccctaaaact tgtccattat
    15721 ttcaaaattt gttatagtct ttttattcaa agcgtaaaag gagatcctat gttaaaagaa
    15781 aaaatgaaaa ctctccccat gctgccgggt gtctatctta tgaaagaccg agatggtaag
    15841 gtcatctatg ttggtaaagc aaaacggctc aaaaatagag taagcagcta ttttcatcag
    15901 aataaacagc attcaaaaaa agtattacgt atgattcatc atataacaga ttttgatttc
    15961 gtcgtagtag acacggaatt agatgcttta ttgctcgagt gtcaattgat ccaacattac
    16021 cgtccttttt ataaccgaca aatgaattat ttcagtaatt ataattatgt acatattacc
    16081 aataagggat ttgtattaac agatactcct acggctcgaa catacgggcc ttttcgtctc
    16141 tataaaaaaa tgccgtctat tttgcgtata atggaagaaa cttatcaaat gccttggttg
    16201 tctgaaatta gtttgttagc tttgcgagtc cagcttcctg acctacagga aatgactttt
    16261 gaacagaaaa aaaaggaact ccaagggctg tttcaaggcc gaaataaaaa gctactgact
    16321 tacttgaaaa aaagacaaca acatttcatc tatcagttga atttcgaaaa agccggtatg
    16381 cttcaaaaag atatcgaatt agttacttat tttattagac ggatccagga acaaaagcag
    16441 tttttaagaa caccatcgct caccttcagc atgccattag cagcagatga gagtcagaaa
    16501 aagcactacc tcgtttgtta tggtcaactt gcagaaacaa tgattacctc tggtgacgtt
    16561 tctcctgact tctattatga aaagaaagaa gctcatttat ctttgaaacg tcaattgtca
    16621 aaagaagaaa tcgatcctgt acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa
    16681 cagtttgaaa ttgaactgtt aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt
    16741 ctcggcttct tttaggcatc ttcaacttca aatgtcaatt atcctaaaat ttattggatt
    16801 aagtcataga tttccatcgc tacgatatcg atattatcaa attcgtattg ctgatgagta
    16861 tctgtttcag ttaattcaaa tgtatcggtc gctttatcat aagttactgt acacttctct
    16921 acgccttctt tttcaaaacg gcgaacttgc agttcattat ctgctgactc gaccatagcc
    16981 tccagtcgtt ttatgattgc gactaattgt gaatgtgtca tcggaggtcc tccttttctt
    17041 atttctgtta tattgtatca aaagcatagc ctacttgcat tgattttctc taaaaaaact
    17101 cgttttttat gatttttttt ctggtatttt tccttcaggc caccaaagtt tgatcaaagc
    17161 ttgggttccg tcattctcaa aacctttatc tgccaactct tcatatagtt tcaatgcttg
    17221 ggtagttgct ggcaatacaa gatccatttt ttctgcttct tctaatgcaa tctttaaatc
    17281 tttgataaaa tgtttgacaa aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga
    17341 ataattgctt aaagaccagt tagctgcact tccacctgaa agcgtctcaa tcactttctt
    17401 taaatcaagt ccgttttttt gtgcatagac aagcatttcc gtcattcctg tcatcgtacc
    17461 tgcaatcatg atctgattag ccatttttgt atgctgacct ttcccagcag aaccatgcaa
    17521 tgtaaatgtc gtaccaaatt ctttgaataa aggcagtact tgatcataaa cggcttcttc
    17581 tccaccgacc atgatcgtca gtgtaccatt ctttgctcct aagtcaccac cggataccgg
    17641 tgcatctaac gcatctgcgc ctttttcttt tgctgttttt gcaatttttt ctgccaggct
    17701 tggtgtactt gtagtcaaat caactaaaat atgtcctgag atatctgcag aaaaaatccc
    17761 cgactgtcca tagtagattt cttctacatc tttggggtaa cctaccattg taaagagaat
    17821 attactttgt tctgctaccg cttttggtgt atctgcccag atagctcctt ctgttactaa
    17881 aggatcagct tttgcttttg tccgattata gacagctact tcgtgtcctg cttctagtag
    17941 atggcgtgcg acagcacttc ccatgacgcc tgttccgata aaaccaagtt tcattttttc
    18001 gacctcctaa aaatcaaaaa tccttgcttc tatgctagta tacgctagaa acaaggattt
    18061 tttcataaaa aatgtttgaa ttttatcttt taaatgaatt taattttatt ttgattcgtt
    18121 caatcgagtc ttcttcttct tttcttcgca tctcttcttc atgataaaca accatgtctt
    18181 gataaaattc gatcatagct tcgccgaaat gtgtagatga aatatcataa agtttagctt
    18241 ctaaaacttt tggatcttta ggaacttcac tggaaatata atcaataagt gctggtgcaa
    18301 agtcttcgtc ggtttcaaaa gttttcccta atgaaggatg atcgaacaat cggttcaaat
    18361 aagcatttcc ttccacgaca caaggtgttc cggctgccat cgcttctgta taagtaagcc
    18421 cttgtgtttc tgaagtagaa gcactgacaa aataatctgc cgctttataa taaagtgcta
    18481 catcattgtt gtccacttct cctgtgaact gcacaaattc ttctaactgc agttctgaga
    18541 tcaattcctc taactcttct acataaggtc cttttcctac gataactaat ctggcgttag
    18601 gataagcgga gatgacctga ggaaacccat taatcaaggc ttggatattt ttctcataag
    18661 aaatcctgct taaagaaaga atcatcaaat ggttttcctg aagacctagt gactccctta
    18721 atttgctgat atcctgctga gtgatatcag aacgtttgaa tttttcaatg tcgataccag
    18781 taggaatcac gcgaagcggt gctattacac catagctgcg taatgtatca ataacacgtt
    18841 cacttggaca aactacacct gtcgtatgat ttgcaaataa tctagacaag tattttacat
    18901 gggattgcct gatcactttt cctttaccga tataatgaag gtagtcttca tacatcgtat
    18961 ggtaagtatg gatcagaggt attttcaact tcttagctac catcttccca agtatccctg
    19021 ctccgaattc tgtatgggta tgaataagat ctagttctag ttctttggca accaaataag
    19081 cataccacat gcctctaaca acaactcttc gatccttgaa agaaataaat ggtacacttg
    19141 gcattcgaat cacatctttt tccaacttat ccgcattagg atcggtcgtt gtaaaaatat
    19201 agacttcatg cccatgtctt tctaattctt gttttaatgt tttgatcgat gtagcaactc
    19261 cgctgacttg cggaaagtag gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca
    19321 aactagtcct tgacctgatt tacttttttt aatttgctag agactttttc attcagtaca
    19381 ctttcgtata catttttaag ttcttcaccg attttattga tacttcgctc ttttgctgtt
    19441 tgataaccgg cttgtgtcgt actcggtagt tcttgattgg taatctgttg gatcagtcgg
    19501 ataaattctt cgtttgtctt ccccatatag cagttcttgc tatcttctaa ccagccatta
    19561 tacactggaa tatctcgaac aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg
    19621 atcccttctg tctcttcata agaagggaaa aagaatagat ctgcacctga atacgcacct
    19681 tctatgatct ctccacgaat gtaaccagga aacaggacat tttcaggatg atcttctttt
    19741 acaattttcc ggatgttccg aggaatcgag tacatgggaa catgaccaaa ccaaataaat
    19801 gtgtattcgg gcatcttctt agcaatttct acgaaatcaa tgattccttt acgttcgaaa
    19861 aacaaaccga cacaaatgat tactttttga tccggagaca gtttgaaata ttctctgaac
    19921 ttttgttctt tcattggatc agggtgatat ttctctaagt cgatcccatt ggaaatagct
    19981 tgaatcggca cacggatacc atagctttcc aacaactgtt ttgaataagg tgttggcgtg
    20041 atcagatagt ccgctttttg gtacaatcct accagatatt tttttacgat aggagaaagc
    20101 tggttcgatc cgataaagga atttcggaaa tcttcctctg tagaatgcgc atgatagaca
    20161 actttttttc cttctcttct ggctttattg accatattat gactattgat tccgtatgta
    20221 ttgatgtgca ggatgtcata atcttctttt tcatctagtg tataagagat accgacttcc
    20281 gtcagagcac gtttttgatg atcaagcgca cgcccgattc cagattttgc aagcatcttt
    20341 tcactttcaa aatataataa taccttcacc ttacgtcccc cttcacttat atagtataac
    20401 atagcctaca aactgtcact caaacttaag ccctattttt gtctattctt actgacaaaa
    20461 acgttttctt aaaaaagaac aaaaaaactg gcgtagctct tttacaagcc acaccagctt
    20521 ttgatacgtt gtttatttta catattcatg aacaagttca acgacttctt ccatcgtatc
    20581 gcattctttc aatgcacgat cagcaagttc agccattttc gctgtgtcca agcgtttcat
    20641 taaactacgc gttttaagaa tagatgtcgc actcattgag aactcatcta gacccatacc
    20701 tactagcaat ggaacagctg tttgatctcc agccatttct ccacacatac ctgcccattt
    20761 tccttcagcg tgtgctgcat caattacatt tttgatcaaa cgtaaaattg atgggttata
    20821 tggttggtac aagtaagaaa cgcgttcgtt catacggtct gctgccattg tgtattgaat
    20881 caaatcgttc gtacctacag agaagaaatc tacttctttt gcaaacttgt ctgctaagac
    20941 tgctgctgct ggaatctcga tcatgatgcc gacttggatc gtatcagata cttccttgcc
    21001 ttcacttatc aatttttgtt tttcttcttc aaagatcgct tttgctgcgc ggaattcttt
    21061 gagtgtagct accataggga acatgatacg taaattacca tgaacagatg cacgaagcaa
    21121 tgcacgcatt tgtgtacgga acatttcgtc gccttgttca gataaactga tacgcaatgc
    21181 acgatatcct aagaatggat tcatttcatg aggtaattgc agatatggta gttctttatc
    21241 tccaccgatg tccattgtac gaacaactac aggttttcct tccatacctt ctaatacagc
    21301 tttgtaagct tcatattggt catcttctgt tgggaagtct ggagaatcca tatacaggaa
    21361 ttcagtacgg tacagaccaa ctgcttctcc accattatta tggacaccaa ctagatcttt
    21421 tggtgttccg atgtttgcag ctaattcaaa atgcttgccg tcagctgtga cagtttctgc
    21481 attttttaat ttttcccatt ctgctttttg tgctgcgtaa tctgcacctg ctttttcaaa
    21541 ttcagctttt tgttcatctg taggatcgat gattacatcg ccttcgatcc cgttgacagc
    21601 caaaatatcg ccttctttga ctttagcagt aatttctttt gtcccaacga ttgctggaat
    21661 ttctaaagaa cgtgccatga tagcagaatg tgaagtacgt ccaccaatgt ctgttacaaa
    21721 cgcttttaca aaattgcgat ctaattgtgc tgtatcactt ggagtcaagt catgtgcaac
    21781 aacgaccact tcttcgttga tcattgatgg atttggtaaa gtaacgccta ataagtgcgc
    21841 taaaatacgt ttagcaacgt cacgaatatc tgcagcacgc tcttgcatat aagcattatc
    21901 ttccattgct tcgaacatac cgatatacat atccgttact tcttttagcg ctgattcagc
    21961 atttacgctg ttgtctttaa tattttgctt gatttgacct accatttctg gatctgaaag
    22021 aaccatcaaa tgggcatcaa atacttgcgc ttctgcttca cctaagcttt gcgctgcttt
    22081 ttcacgaatt tgctgcaatt cttcagtaga tttagctaat gcagcatcca aacgagcttc
    22141 ttcagccgca gtatcttcta ctgttgcctt actgaatgtt aaatccggtt gaactagcag
    22201 gtaagctttt gcaacggcta caccgtcact agcggcaatc ccttttagca tttcaaccat
    22261 tattcagata agccttcttt cttcattgtt tcaacgattg ctgccattgc atcagcttcg
    22321 tcagcacctt ctgcagtaat agtaacatca gaaccttgac ctacacctaa agacataacg
    22381 cccatgattg atttcaagtt tactgattta cctttatact ctaagtttac atcagagtta
    22441 aatttgcttg ctgtttgtac taatagtgta gctggacgag cgtggatccc agtttctgct
    22501 actacgtgaa attctttctt ttccatatta atcggtctcc tttaagtgag tgttgttttt
    22561 atgtgagggt tttctttttt agtaattcag agaatcctaa caaatgccac cctttcatct
    22621 gtaaagatta acattttttt tcgttagata caacagtttt cattcaattt cagataaaag
    22681 tattcaaatt attccggatt ctttttcatc cttttcttca cattttataa caaacgtgtt
    22741 tatttccttt ttcttgtcgc tttctcttct cctgacgttt atcattcttt cctttgaccc
    22801 tctttgtttt catatgaaac gaattacttg cttccctttt tatttttagt ataataagag
    22861 tcaaaggtca ggaatggtca atagagattt caccttttct acctcataaa tctcttaaaa
    22921 aactaggggt tattctttta cctcatgaaa aacgagatgt aaaatatgga tgtacccatt
    22981 tttgtgaaag gacgtgaatg attcatgctt tgtcaaaact gcggcaagaa tgaggcaacg
    23041 attcacttgt atgccactgt caatggtcag cggacacaac tcgattactg tcagagctgt
    23101 tatcaaaaaa tcaaaaatca acaaaatgga ggtctgacta atatggccca aaatgatcca
    23161 tttggatttg gcagtttgga cgatcttttc cgttccatgt ctcgccaaat gcaacagcaa
    23221 ggaaattatg agcaaacacc accaacacaa tttggcggaa acaacaactt caatggtggt
    23281 cagccacctc aaggcaatac agaaggttta ttgggagaat acggaatcaa tatcacagaa
    23341 caagcacgac aaggtgatat cgatcctgtg atcggacgcg atgacgaaat caaacgagtc
    23401 attgaaattt taaatcgccg aaccaagaac aatcctgtct tgattggaga accaggtgtt
    23461 ggtaaaacag cagttgttga aggtttagca caaaaaatcg tagatggtga tgtaccgcaa
    23521 aaattaatgg acaaagaagt cattcgttta gatgtcgtat ctttagtcca aggtactggt
    23581 atacgaggac aatttgaaga acgtatgcaa aaattgattg aagagatccg tcaagcagaa
    23641 aatgtgatcc tattcataga tgaagttcac gaaatagtcg gtgctggttc tgctggtgat
    23701 ggaaatatgg atgctggtaa tattttaaaa ccagctttag ctcgaggaga attgcaaatg
    23761 gttggtgcta ctaccttaaa tgagtaccgc atcatcgaaa aagacgctgc tttagaacgt
    23821 cgtatgcaac ctgtacgggt agatgaacca actgtcgaag aaacgatcag tattttgaaa
    23881 ggtttgcaaa aacgttatga agactatcat catgtaaaat atactgatga ggccatcgaa
    23941 gcagccgcaa ctttatccaa tcgttacgtc caagatcgct tcttgccgga taaagccatc
    24001 gatttgctag atgaaacagg ttctaagaaa aacttgacga tccaaatcgt tgatcctaaa
    24061 acaatcgaga aaaaactgca aaaagcagaa gaacaaaaag ttttagcttc tcgagaagaa
    24121 gatttcgaaa aagcagctta ttatcgcgat cagatcaata aacttcaaaa aatgaaagaa
    24181 cgtcagctca ctgaagagga aacacctgtg attaccgaaa aagatatgga aaaaatcgtt
    24241 gaacaacgga caggtattcc agtgggtgaa ttaaaagaaa aagaacagac acaactgaaa
    24301 aacttagcag atgacttgaa agctcatgtc atcggacaag ataacgctgt agatcgagta
    24361 gcgaaagcga ttcgccgaaa ccgtgttgga ttaaacaaac agaatcgtcc aatcggctcc
    24421 ttcttatttg ttggaccaac tggtgtaggt aaaacagagc ttgcgaaaca acttgcctac
    24481 gaactattcg gttcacaaga ttcaatgatt cgtttcgata tgtctgaata tatggaaaaa
    24541 catagtgtct ctaaattgat tggttctcct ccaggttatg tcggatacga agaagctggt
    24601 caattaacag aaaaagtacg gagaaatcct tacagtcttg tcttgttaga tgaagtagaa
    24661 aaagctcatc cagatgtttt gcatatgttc ttgcaaatct tagatgatgg tcgattgaca
    24721 gatgcacaag gaagaacggt cagctttaaa gatacgatca tcattatgac aagtaatgcc
    24781 ggaactggta aagtagaagc aaacgttggt tttggtgcgg cacgcgaagg tgttactcgt
    24841 tctgtattga accaattaaa caactacttt acaccagaat ttctgaaccg ttttgacggg
    24901 atcatcgaat tcagtgcatt atcaaaagaa aacttgatga caatcgtcac actaatgcta
    24961 gacgatgtca accaaatgct ggcagcacag caacttcaca tcgaagttcc tacgaatgtc
    25021 aaagaaaaat tagttgactt aggctatgat ccatctatgg gtgctcgtcc tcttcgacgc
    25081 acgatccaag aacaaatcga agatggtatc gctgaatttt atcttgatca cccttctatc
    25141 catgagttga aagcaaaact ggataaagat ggaaaaatca tcgttacttc taaaccagaa
    25201 cgtttggcta aagaatcagc cgaagaaacg gctgaataaa tccagtttta tagcgtgaga
    25261 cggtatttct ttgagaaatg ccgtctcatt cttttttcag aaaagtcaaa atactgttca
    25321 ttttataaaa aagaaattat aataagagaa acaagcaatt aaaaatacat aaaagaggtg
    25381 agcgccatga aattggtaaa tgtaacaaac agccattccc gtcttgtatt aaaccagctt
    25441 gaaaacactg atgcccatct agtcaaggtc tacacagccg gaaataccac tattgtctac
    25501 actgaagccc ctgaacataa tgaaattctg ctgatcaatg acaaacgtaa aatccagccg
    25561 aaagaaatcg aagatgctaa aaattatttc ttagataaaa tcaaagacgc cgtttaccac
    25621 gaggatgaaa tcaaagtaat cgaattggca ggccttgttg aaatctcgat tccaaaacat
    25681 gaattaggcg ctacattagc cgtgtaaaca aattttgaac ataataatta aagagcgtgg
    25741 gacaaaag
//
LOCUS       DACBZN010000031        25256 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.031, whole genome shotgun sequence.
ACCESSION   DACBZN010000031 DACBZN010000000
VERSION     DACBZN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.031"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            160..1101
                     /locus_tag="IVD64_001267"
     CDS             160..1101
                     /locus_tag="IVD64_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP6148527.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1170..1901
                     /locus_tag="IVD64_001268"
     CDS             1170..1901
                     /locus_tag="IVD64_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP6148528.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2045..4225
                     /locus_tag="IVD64_001269"
     CDS             2045..4225
                     /locus_tag="IVD64_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP6148529.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4245..4727
                     /locus_tag="IVD64_001270"
     CDS             4245..4727
                     /locus_tag="IVD64_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP6148530.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4743..5504
                     /locus_tag="IVD64_001271"
     CDS             4743..5504
                     /locus_tag="IVD64_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6148531.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5635..6969
                     /locus_tag="IVD64_001272"
     CDS             5635..6969
                     /locus_tag="IVD64_001272"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP6148532.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7276..7761
                     /locus_tag="IVD64_001273"
     CDS             7276..7761
                     /locus_tag="IVD64_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP6148533.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7754..8437
                     /locus_tag="IVD64_001274"
     CDS             7754..8437
                     /locus_tag="IVD64_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP6148534.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8563..11208
                     /gene="polA"
                     /locus_tag="IVD64_001275"
     CDS             8563..11208
                     /gene="polA"
                     /locus_tag="IVD64_001275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP6148535.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11253..12089
                     /gene="mutM"
                     /locus_tag="IVD64_001276"
     CDS             11253..12089
                     /gene="mutM"
                     /locus_tag="IVD64_001276"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP6148536.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12053..12682
                     /locus_tag="IVD64_001277"
     CDS             12053..12682
                     /locus_tag="IVD64_001277"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP6148537.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12734..13066
                     /locus_tag="IVD64_001278"
     CDS             12734..13066
                     /locus_tag="IVD64_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6148538.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13378..13869
                     /gene="nrdR"
                     /locus_tag="IVD64_001279"
     CDS             13378..13869
                     /gene="nrdR"
                     /locus_tag="IVD64_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP6148539.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13892..15265
                     /locus_tag="IVD64_001280"
     CDS             13892..15265
                     /locus_tag="IVD64_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP6148540.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15265..16191
                     /gene="dnaI"
                     /locus_tag="IVD64_001281"
     CDS             15265..16191
                     /gene="dnaI"
                     /locus_tag="IVD64_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP6148541.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16320..16760
                     /locus_tag="IVD64_001282"
     CDS             16320..16760
                     /locus_tag="IVD64_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6148542.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            16904..17605
                     /locus_tag="IVD64_001283"
     CDS             16904..17605
                     /locus_tag="IVD64_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP6148543.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17602..18723
                     /locus_tag="IVD64_001284"
     CDS             17602..18723
                     /locus_tag="IVD64_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP6148544.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            18738..19970
                     /gene="pepT"
                     /locus_tag="IVD64_001285"
     CDS             18738..19970
                     /gene="pepT"
                     /locus_tag="IVD64_001285"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP6148545.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20102..21010
                     /locus_tag="IVD64_001286"
     CDS             20102..21010
                     /locus_tag="IVD64_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP6148546.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21045..21317
                     /locus_tag="IVD64_001287"
     CDS             21045..21317
                     /locus_tag="IVD64_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP6148547.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21328..22374
                     /locus_tag="IVD64_001288"
     CDS             21328..22374
                     /locus_tag="IVD64_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP6148548.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            22625..25234
                     /gene="clpB"
                     /locus_tag="IVD64_001289"
     CDS             22625..25234
                     /gene="clpB"
                     /locus_tag="IVD64_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP6148549.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT         8565 a         4398 c         5545 g         6748 t
ORIGIN      
        1 ttctgtgcga aacaggaaag cgaagtcttt ttaattcaaa taataatatt atgtaaacaa
       61 aaaactttcc atgcgtaatt atacacaagc aaataaatcg tcaaatgcga acgccttctt
      121 tatactgttt aggaacgaaa agaaggaagg aagaaataca tggattatca agtactgctt
      181 tattacaatt atacaaaaat cgaagaacca gaggaatttg ccaaaaatca tttagccttt
      241 tgcaaatctt tggatcttaa aggtcgtatt ctagtagctg aagaaggaat aaatggtact
      301 gtgtctggaa ccgttgaaaa cacgactgcc tatatgaaag caatgaaagc tgatcctcga
      361 tttgctgata cgatgttcaa gatcgatcca agcgaaggac atgcctttaa gaagatgttt
      421 gtaagatacc gtccagagct tgtaagttta aagctagaag aggacattga tccaaaccaa
      481 gtgacaggtg cctatctttc accgcaagaa tttaaagaag ctattttaga tgaacagaca
      541 gtggtgattg atgcacggaa tgattatgaa tatgacttgg gacattttcg aggggctgta
      601 cgcccagata tacgcagttt tcgagagctt ccagactgga tccgcgagca caaagaagaa
      661 tttatggaaa aacgggtagt gacctactgt actggtggca ttcgttgcga gaagttttct
      721 ggttggcttg tccgtgaagg attcaaagat gtcggacagc tgcatggcgg tattgctact
      781 tatggtaagg atcctgaagt ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat
      841 gaacggatcg ctgtcgatat caaccaagta gataaacaaa tcgtcggtcg cgactggttt
      901 gacggaacac catgtgaacg atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt
      961 cttgcttcca ttgaaaatga agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa
     1021 catccgcgca atcgctatgt tcaagaccat ggaatctctc cagaagaagc agaactgcgg
     1081 tttacagctt taattaaata gaaaacaata gttttgattg tcgaccattt ccaatatgcg
     1141 ctatactagc gttgaggatg gtgatccaca tgaaatcatt gatttatgct attggggatg
     1201 tccatggcaa acatgagctt tttgaaaaac ttattcacta ttatgatcca aagatccatc
     1261 agcttgtttt attaggggat ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat
     1321 taggaaagca attagtcgaa gaaacaggag ccatttacct ccggggaaac catgaagaat
     1381 attttttgca atttctgcaa tcaccggagg actggtatgc gccttatatg agaaatggtg
     1441 gaaaagaaac gtttgaaagc ctgattcacc caggggcatc ggaggaatac tccccaactg
     1501 aaatctcgat gatgatccaa tcgagatatt cagaactgat taaattttta gtccagcgcc
     1561 ccttgtatta tgaatgggaa aattatcttt ttgtccatgc cggtgtggat ttgacaaaga
     1621 aagactggcg taagacaagt ctaagagact ttatttggat aagggaacca tttcatgaag
     1681 ggaaaaacaa tacaggtaag acaattgtat ttggacatac gattacacca atgctccatg
     1741 gagatatgca gacaacagac ctctggattc aggatcataa aatcggaatg gacggtgggg
     1801 ctgtattcgg agggtcattg catggggttg tttttgacaa ggaaggtatt cttcaagata
     1861 ttgaatatca gaacaacgaa ggaccttggc atggaatctg actaatggaa agaatgaagg
     1921 agctgtgact ttgttgcggc tctttttatt tttatagaga atattgtttc tttcagtagt
     1981 ttaacccagg ataaaaagta tgatagaata aagaagcaat ttttagcaga aaagaggcat
     2041 tttaatgacg gaaaatttga accagacgat cattcagctt gtacaaaaag aactttcaga
     2101 ctatcgtcct aagcagttga ctactgtact taatttgtta aatgaaggaa acactgttcc
     2161 ttttatcgcg cgttaccgaa aagaaatgac tggaagtctt gatgaagtcc agattcgtga
     2221 aatcgaagag cggtatgcct atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg
     2281 gctaatcgat gaacaaggga aactgactcc cgagttagaa gcagaaatca cccaagccgt
     2341 gaaaatgcaa caagtggaag atttgtatcg tccatacaaa caaaaacgac ggacaaaagc
     2401 aacgattgct aaagaaaaag gacttgaacc actcgcgaag tggctgatgc agcttacaga
     2461 tggagaggtg caatctgaag cagaaaaata tattgacaaa gaaaaagaag tctcttctgc
     2521 cgaagaagcg ttgcacggtg ctcatgagat tattgctgaa caagtcagcg ataatgcaaa
     2581 atttcggact tggattcggt cttatacgta taacaaaggt atgtatgtca gcaatgtcaa
     2641 ggatgagcaa gcagatgaaa aaggcgtata tgaaatgtat tatgactttg cagaacccgt
     2701 ccataaaatg gtttctcatc gaatattagc tactaatcgt ggagaaaaag aagatattct
     2761 gaaagtattc tttcaagtag atgaagcagc aattttagct tacttggatc gccaaatagt
     2821 caaaaatcct gcctctcctt cttcttcatt cgtcagagaa gcctatcagg atagttataa
     2881 acgctttatt cagccagcaa tcgagcgaga actgcgcaat gaattgactg aaaaggcaga
     2941 tgagcaagca atcgctattt tcggcgaaaa cctccgtaat cttttgttgc aaccgccact
     3001 aaaaggtaaa gttgtcctgg gatttgatcc ggcttatcgt actggatgta aactagcagt
     3061 cgtagatgcg acagggaaag ttttagcaat cgaagttatt tatcctcata aacctgcagc
     3121 gcaagcaaaa agagaagctg ctggaccagc ttttatccaa ttaattaata aatatcaagt
     3181 agatatggta gctatcggaa atggtacagc cagtcgggaa tctgaacttt ttgtggctga
     3241 gcagttgaaa tccgccgacc ataaaaccta ttacgctatc gtcaacgaag caggcgcttc
     3301 tgtttactcc gctagcgaaa ttgcacgaaa agaatttccg catctacaag ttgaagaaag
     3361 aagtgctgtt tctattgccc gtcgcctgca agatccttta gctgaattag tcaaaatcga
     3421 tcctaaagct gtcggagtag gccagtacca acatgacgtt tctcaaaaac gtttggctga
     3481 acaattggat tttgttgtgg aaacagcggt taaccaagta ggtgtggatg tgaatacagc
     3541 aagcccgcag ctcttgcagc atatatcagg attgaataaa acaactgccc aaaacattgt
     3601 catttatcgt gaagaaaacg gagaattcac cgcacgaact caattgaaaa aagtgccacg
     3661 cttaggacca aaagcatatg aacaagcaat cggattcttg cgagtccctg gtgggaaaaa
     3721 cattttggac aacacaggta tccatcccga aagttattct attgcaaaag aaatattaac
     3781 ttcagtccaa ctttcagaaa aagagcttgg tacagaagaa gcagtagaaa aattgacacg
     3841 attatccgta gagaaactgg ctgaaagcct atcagtcgga gaagaaacgt tagccgatat
     3901 tttagctggg ctgacacaac ctggacgaga tatgcgtgat gagatgccag cgcccttatt
     3961 acgtacagat gtattgagta tggaagactt gaaaccaggg atggaattga ctggaacggt
     4021 ccgtaatgtc attgacttcg gcgcatttgt tgatatcggt gtgaagcaag acggacttgt
     4081 ccatatatcg aaactcagca aaaaattcgt caagcatcct acagatgtcg tgtcagtagg
     4141 agatatcgta acagtttgga tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac
     4201 gatgctttcc ccttatgaag agtaacaatg aggagtaaca aaaattgaca caagaagaac
     4261 tgcagcagtt agtagaagag atatcattaa aaagcttcca gcttcctttc aagcaccaag
     4321 caatgttcaa tccacgctta cgtacaactg gcgggcgcta tcatttaaac gatcatcatt
     4381 tagattttaa tgcgaagcta tttgaagagt cagacgaaga gacgattgca ggaatcatca
     4441 aacatgaact ttgccattat catcttcatc tagctggaag agggtatcag cataaagata
     4501 tggatttcaa gtatcttttg aaaaaaacag gagggttgcg ctatgcacca gccttgacga
     4561 agaaacagat aaagatggag tattatcgat gccaagattg ttctgccacg atctgccgca
     4621 agagaagaat cgatgtacgc aaatatcgtt gtggaagatg tcatggagaa atcaaatgga
     4681 ttgagacaaa aagagaagac tttaatgaag cacaaaaaat cagatgaaag gatagaaggc
     4741 ctttggaaaa aagtgagcag aggaatctaa aagactggaa tcagctatta aattttttag
     4801 ccgcacctga agagggaaat tttgaggaac tgacggcacc tacacttatt ttagctggaa
     4861 actgtcttcc tatcttagct gataaagcag cacaaatgta tttaaatggc caagtagatc
     4921 aattgttttt ggtcggaggg gtggggcatg cgactagaat cctttatgag aattttgaaa
     4981 agcaaggatt tcattttgaa gatggcatga gtgaaagtga aatctgtcgt cagtatctaa
     5041 aagaagtcta tgatttgccc gataaggctt ttctgataga atcgaagtct acaaattctg
     5101 gagagaacgc cactttttca ctggagattc ttcattcctt aggagctgta ccagaaaaaa
     5161 tcttattgat gaatgatccg acactgcaac gcaggactcg agcaactttt gagaaagtat
     5221 ggcaaaatga acaaactatt ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact
     5281 tttctgaaga gatcatcttt acagcaaaag aactgaatca tcaatggcct aaggaatatt
     5341 tccatgcgct agtattagga gaaatggaac gtctccatga cgatgagaac ggctatggtc
     5401 cgaaaggaaa agattttatt cctcacattg atatatcaga ggaagtttgg tcttcttaca
     5461 ccagaattaa agagtcaata aaaacggatt tttctcgtac ttgaccattt tagcaagcga
     5521 atcctccttt ccaaataaat ttggttgaaa ttttcaatcg tttataagat aatgaggagc
     5581 gtgttgaatg taatagctaa aagaatggac gctagataag agaagaggat tcaaatggaa
     5641 aatcaaaata aattgtatca ttttgttggt atcaaaggtt caggaatgag ctccttggct
     5701 cttgttttac atgaacaagg tctaaatgtc caaggatcag acattgaaaa atatttcttt
     5761 acacaaagag atttagaaaa agcaaatatc acgattctgc catttaacgc agataatgta
     5821 aaaccaggga tgacgatcat tgcaggaaat gcatttcctg attcacatga agaaatccag
     5881 cgagcaaaag aattaggttt ggaagtgatt cgctatcacg actttattgg tcattttatc
     5941 caaaattata cgagtatcgc tgtaacaggc tctcacggaa aaacaagtac gactggactg
     6001 ctctctcatg tattgagtgg tgtccgtcca acaagttatc taattggaga tggaacagga
     6061 catggtgatc cgcaagcgga attcttttca tttgaggcct gtgaatatcg ccgtcatttc
     6121 cttgcctatt caccagacta tgcaatcatg accaatatcg attttgatca tccggattat
     6181 tatacaagta tcgacgatgt atttacagct ttccaaacaa tggctggaca agtgaaaaaa
     6241 gctatttttg cgtatggtga cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt
     6301 tattattatg gtgtcacaga aaacgatgat atccaagcac gaaacatcga acgcacaact
     6361 tctggttctg cttttgatgt ctatcatggg gatgaatttg ttgggcactt cactgtacca
     6421 gcattcggta aacacaatat cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa
     6481 aaactggatc tgaaagaagt tgcagaagaa atgctgactt tcccaggagt aaaacgtcga
     6541 ttcagtgaaa agatcgtagc agacatgacc gtagtcgatg attatgcgca tcaccctgca
     6601 gagatcaaag caacgattga tggcgcacgc caaaaatatc cagataaaga aataattgca
     6661 gttttccaac cccatacgtt tacgcgtacg attgcattga tggatgaatt tgcagaagca
     6721 cttgatttag ctgataaagt ttacttatgc gatatttttg gttcggctcg ggaagaacaa
     6781 ggaaacgtta agattgaaga ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa
     6841 gaaaataatg tatctccatt gttagattat catgatgcag tagtaatctt catgggtgct
     6901 ggagatgttc aaaaatttga acaagcgtat gaaaaacttt taagcagcac gacgaaaaat
     6961 gtactgtaat acaattactg cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa
     7021 atcttgccaa gccccctttt ttgcaactta tgataaacgg tgttcaaaag cttgcatctg
     7081 cggtaataag cccaatcaaa tcaaaaacat gagaagctgt tttttcttgg ctgttcttat
     7141 tatttgatgc aggacagctt tttcacgacc tctgtaaact ataggaatct gttaaatcaa
     7201 gggagcagaa cttgcctttt acgctacttt tggtacaata atgtatgatt aaaaataaac
     7261 tggagggttt ctatcttgaa cataggtaag ccgagaaaac taggaaaaac aataaatgaa
     7321 atcgaggaag gcgactcgtt atccttaaca gaatctattg aagataacca gcttttgctg
     7381 tatttgggat tgacgaatga tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa
     7441 acagaatatc aacagccaat cgttccatca gtgatgctga tggggatcat cacaagtgca
     7501 atttcaaagc atcttcctgg accaggatca catgttgtca atttttctgt caattttatt
     7561 gaaccggttt tccattatga aacactgact tttcagtttg aggtcataaa agtagataag
     7621 atgaaggatg tggtgacgat ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg
     7681 gatgctgtcg ttatggtcca accgccacaa gttcctgaat tggaaatagc aatcgaaagt
     7741 gaaggtgaat tgaatgaata atccaagtac atcaatgtca ggtatcaatc gtttttatgc
     7801 aaaagtttat ggatttttag gtatgggact tgcccttagt gcagtcactt cttacttggc
     7861 tctttgggta tttcctttgc aattagcaac ttttatcaat aattttccac taggctttgt
     7921 gggtctgtgg atcgttgaac tgattttagt aatggttcta ggcgtaaaag cacaaaagaa
     7981 tccgagctta gcaatcggtg gatttattgc ctactctctt ttgaatggca taacattagc
     8041 ggttactctt gcatactacg atattggaac agtaacgcaa gctttcttga cagctacttt
     8101 gatgttcatg gggatgtctg ttgtcggagt aacaacaaaa agagatctta gcggggttgg
     8161 aagagcagga caggcagctt taatcggagt gatcattgca ctagtcttga acatcttcat
     8221 tttgagaagt agcgtagttg agctgttcat ctccctactg ttagtcgtca tctttgcagg
     8281 gattacagct tatgataacc aaagcatccg taatatctat gtccagacaa acggaacagc
     8341 acatagtgga attgctgtat tcatggcatt gaagctgtat ttggacttta tcaacttgtt
     8401 cttggcgttt ctgagaatct ttggacggaa caactaaaaa agaaaaaaat tcatcagttg
     8461 gggctatacc gaattatttg gtatggtccc gctttttctt ttcgcattcc gcaggattct
     8521 ttgttagaat gaataagaac tttacagtaa ggagttaaga agatgacaaa aaataaatta
     8581 ttactcgttg atgggaacag tgtggctttt cgcgcattct ttgcgcttca caattctctt
     8641 gaacgtttca aaaatagaaa cggtcttcat acaaatgcaa tctatgcatt caatacgatg
     8701 tttgaaaatg ttatgcaaaa agagcaaccg actcatgttc tggttgcgtt cgatgcagga
     8761 aaaacgactt ttcggacaga aatgtatgcg gagtacaaag gtggaagatc caaaacacct
     8821 ggcgaattca aagaacaaat gccatatata cgtgatttgt tgacaggact aggggtacag
     8881 tattacgaat tgcccaatta tgaagcagat gatattatcg gtactttggc agaaaaagta
     8941 gacaaagatc aatttgatgt cgtcgtgctt tcaggtgatc gggatttgac tcaattagct
     9001 agtaaagaag taaaagtgga tatcactgta aaaggtgtca gcgatatcga aagctataca
     9061 ccagaacatg tagcggaaaa atatgatggg ttgacgccaa aacagattat tgacatgaag
     9121 ggactagccg gagatgcctc tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca
     9181 gctatcaagc tattgaaaca atatggctca gtcgaaggaa tttatgaaca tatcgatgaa
     9241 atgaagcaaa gcaaaatgaa agaaaatctg ataaatgaca aagagcaggc tttcttatcg
     9301 aaaaaattgg cgaccatcaa tacttcagcg cctgtggatg tctctatcga ctcattgaag
     9361 tatgaaggga aaaatctcga taaactcgtt ccgttttata aagaaatgga tttcaatcag
     9421 ttcctttcta aattgaatat cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa
     9481 gtcgttcatg aattcaaaga ggaaatgttt actacggata tggcgttgta cgtggaaatg
     9541 atgggcgaca attatcatac agaggaaatc gtcggagtag cgtgggggac agaaaagaaa
     9601 atttatgtca cgaacgatct atctgtattc gaaaaccgag cgttccattc ttggatcact
     9661 gatccgactc ggctgaaaaa agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc
     9721 tatactggaa aaacgaaagg aatcgatttt gatgtcttgc taggtgcata ccttctcgat
     9781 acgaatgaaa aaagcacaga tatcgaagga atagctgctc attacggata caacgatatc
     9841 caatcagatg aagctgtata cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa
     9901 ctcttttttg ctcatttagc gcgaaaagta gctgcaatca atgcgttgac accaaaattg
     9961 agccaagaac tggcagataa aaaccaagaa gatcttttcc ggaaaatgga actgccattg
    10021 tctatcattt tggcagagat ggaaataagc gggatcaaag tagatgcgac tcgcttacaa
    10081 gaaatgaagg gcgagttctc tgctcgctta agagagatcg aacaaaaaat ctacgaagaa
    10141 gccggcgaag aattcaattt gaattcacca aagcaattag gagtgatctt atttgaaaaa
    10201 atgggattac ccgttatcaa aaaaacaaaa actggatatt ccacagccgt agatgtactg
    10261 gaacagcttc gtgaacaagc accaatcgta gaggatatcc tcacctatcg tcaaattgca
    10321 aaaattcaat ctacctatgt cgagggactg ctgaaagtga tccaatcaga tggaaaagtg
    10381 catacccgtt atgtccagac attgacccaa acaggacgat taagctctgt tgatcctaat
    10441 ttgcagaaca tcccgattcg attagatgaa gggagaaaga tacgtcaggc atttgttcct
    10501 agagaaaaag actggctgat ttattcttct gactattcac aaatcgagtt acgcgtactt
    10561 gcgcatatct caaatgatga acatttgaaa gctgccttcc tagaaggtca agatatccat
    10621 gccagcacag cgatgcgtgt atttggaatc gaaaaggcag aggatgtcac accaaatatg
    10681 cgtaggcagg caaaagcagt gaattttggt atcgtgtatg gcatctctga ttacggactt
    10741 tcacaaaatc taggaatcag ccgaaaagca gcacagcaat acatcgatac gtactttgaa
    10801 aaatacccag gtgtcaaaga atatatggaa agaatcgtac gcgaagcaaa agaccaaggc
    10861 tacgtcgaaa cgctgtatca ccgccgtcga tatctgccag acatcaattc aagaaattac
    10921 aatatccgtt cttttgcgga acgaacagct atcaatacac caatccaagg aagtgctgct
    10981 gatattttga aaatcgcgat gatcgaattg gataaacgat taaaagaaac aggtcttcaa
    11041 gctacgatgc tgttacaagt tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt
    11101 gaatcattag ataaattggt gaaggaagtt atggaacaag ctgtttctct tcatgtacca
    11161 ctgatcactg acagcagttg gggaaaaacg tggtacgaag caaaataaac attgtttaag
    11221 acagagcaat ataatgaagg tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg
    11281 ttcgtaaagg attggaacgg ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt
    11341 ggcccaagat catcgagcaa ccagagacac ccatctttga ggcttctctt gttggcgaaa
    11401 ccatccaatc cattggacgt agagggaagt ttttaatttt tcatctcgat cattgtgaac
    11461 tgatctcaca cttgcgaatg gaaggaaaat atcaatttac gaaagaaaat acaccaatcg
    11521 ataaacatac gcatgtactt ttcttttttg aggatggaag ccaacttcgc tacaacgatg
    11581 tacgaaaatt tggtcgtatg acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa
    11641 tcatgaaatt gggacctgag cctttgccag atagcttctt attagcagat ttcgcaaatg
    11701 gactgaaaaa gtcccacaaa gcaatcaagc cattgctgct agaccaacgt ctagtgactg
    11761 gtctaggtaa tatttatgta gatgaagcat tatgggaagc gaagatccat ccagaacaac
    11821 cggcgaacac actgcgcccc aaagaggtag aacaattacg tctatcgatc attgatgttt
    11881 tggatcgtgc aatagaagcc ggaggtacca cgattcgcag ttatttgaat gcattaggag
    11941 aatcaggcgg atttcaagta gcactgcatg tctatcagca aactggcaaa ccatgtatac
    12001 gttgtggaac gccgatcgtg aaaacaaaag tggcacaaag ggggacgcac tattgtccga
    12061 attgtcaaag gctaaaaatc gcaaagtagg atttgtttta ggtttgacgg gtggaatagc
    12121 taccggtaaa agtacggcag ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg
    12181 cgatgtgatt gccagagaaa ctgttgagcc tggaacgctt gctctgagga aaattgtatc
    12241 agtgtttggc caagaggtat tgcagcctga tgggcaacta gatcgaggga aacttgggat
    12301 gattgttttt ccatcaaagg aaaaacggca aaaacttgat cagctattag atccgtttat
    12361 ccgaaaagcg ataaaagatc aaatcgcctc tctaagttcc aatcatcctt tagtaattgt
    12421 agatatccca ttgctctacg aaggtcatta tgaccactat atggatgcag tagctgtcgt
    12481 ttatactacg ccagaaactc agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga
    12541 acaagcgcag cagagaatta gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga
    12601 tattttgttc gacaacaatg gaacaaaaga aaaacttgtg ggacaaatcg aaaattggct
    12661 ttttgctaat cgctttcttt gagtatctgt gtcaattttg ctatcctgag tatatagaaa
    12721 gtgagggata gcgatgggat cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa
    12781 agaaaaaatc ggcgagatgc tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc
    12841 agaaacagca gaagaaaaag aaaagcagta ccaagcaaga atcgatgaaa tagaagacca
    12901 gcaggcagaa gaagaagcga agcggcaaga tttgactcaa gaacgaaaaa atccgacacc
    12961 tgatcgtagt aaaacaccgc tcccagaaag tgaccacatt tccgatgagc aaaaaatcga
    13021 ggaacagcga atgaaacact atacaggaat cgaagagaag ttataaaata gtaaaaaatc
    13081 gagatgtgac ttgaagcaga tgagaaggtg ttcatctgct tcaagttttt tgtgctagat
    13141 gactaaatca gcttttgtgt aagttatttc tcagtaatca aggtgatcag tattggaaaa
    13201 ttgaaattta gtaaggacag gggacacaat tacgcatgaa agtagaaaaa attgttctta
    13261 tatccgtgtt ttttgggtag gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac
    13321 tagttgtaaa gaaagacaca tcctttatat aaaatgcgat gcaagaggtg agacaaaatg
    13381 agatgtccca gatgtcaaca taataattca cgagtaatcg acagccgtca agcagatgat
    13441 ggacgtgcga tccgtcgccg tcgcgaatgt gagaattgtt cgttccgatt cactacattt
    13501 gaacgaattg aagctgctcc gttattagta atcaagaaaa acggggaacg cgaagagttc
    13561 aatcgcgata aaattttaag aggactcatc cgttcagcag aaaaaagacc tgtagctatg
    13621 gaacaaatgg aacaaatcgt caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac
    13681 gaagtatcga cgaatctaat tggtgaatat gtgatggaag atttagttga tttagacgaa
    13741 atcgcttata ttcgttttgc aagtgtttat cgccagttca aagatatgtc tgttttctta
    13801 aaagagttgc aagatatcgt ggataaagca aaagaaaata ataaagagaa taataaagaa
    13861 aacaattaag aaacaaaaag gaggcatttc cttggaaaat gcttggaaag agcttcaacc
    13921 aaaaaatatt taccaaggaa gaaaagcggc tccgctgaga gaagacagca agaacgcctt
    13981 gctgtatctt tatcagccac taatcggcgg agaagccttg agtatctatt tcaccctgtt
    14041 atcagaaatc tctcaagaat ctggcaaagg tcctgaaggg atgcatgcag atctgcttag
    14101 tagtctaggc tgcggtatcc cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg
    14161 cttactggat gtttatttta aagaagatag tgatctggga cctcgtttta tttatgagct
    14221 gttagaacca ctcattccta aagcattttt caaagactct gttctggctt ttcttcttct
    14281 ggaaaaagtt ggtgaacgac gattccagaa gcttacccaa ttctttgaac ctaaaaggca
    14341 tacagcagag ggatatcaaa aaatcacaaa aaaattttta gatgtttatc gcttcaatga
    14401 agcagtgtac gctgctaatc aagaaaacat cgaatcgctg aataaatctt ttgaagaatc
    14461 tgaatcagta gcattacttg aagcagattc gacattcgat tggcagtttt tgaatgattg
    14521 gctggcaaaa aaacatattg caagtcttga tgatccaatc atcaaacaag tcgacatgta
    14581 tcatcagctt tacggtttcg atgaattgac acttggcgaa ctgatcagtc aatcttatga
    14641 ctttagtgaa caaaaagtat ctgaaaaaga actgcaaaaa gccattttgg caatgcagag
    14701 acgtccggtg aagcaaacaa aagaaacagt tgcggtttct gaaaacagta atgaaccctc
    14761 gtcaaacgaa catttgacaa acgcagcgct tcagctgata aaagaagcac agagcattcc
    14821 accgatgaaa tatctggaag caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga
    14881 aaactggtta ctgcaagatc ttgtttctca atcaggtctt tccagtagcg tgatcaatgt
    14941 tttgctgaat tatgtactag tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa
    15001 tacagtggca aatgaatggg cacaaaagaa aatcgccaca gcagaagaag cgatccagca
    15061 cattcggcaa ataagtaatc aagcaaaaca atcaaaacaa aagaagcaaa caaattatca
    15121 aactggcaag cgaaatgttc gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga
    15181 tgaaaaacag atcagcagcg agaaaaaagc agagattgac cgccgtttta aagaatacct
    15241 ggcaaaaaag gagggtgata tctagtggaa gacgtaggca aagaattgaa gaggatcatc
    15301 agcaaacgca attatcaaga aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg
    15361 gatgttcaag cttttctaag cgcacaccaa gaagaattga cacaagcgga tattgaaaaa
    15421 agttacgcta aattgtatga attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca
    15481 acgatgatcg ctcccggcta tgagccgaag ctgacgttga attttcatta tgtggatgtc
    15541 acttatgtac caacggaagc attgttagca cggcaaagac aagaagaaat cagaagccga
    15601 gtaaaagcaa tggatatgcc aaaagatatt caatcggctt cttttgaaaa ctttgaacgt
    15661 acagatggac gaggatatgc gagcttagaa gccctagatt ttgtagagaa atatactgct
    15721 gatccaaaag ggtatcataa aggactgtat ctagtaggaa gttttggtat tggaaaaaca
    15781 tatttattag gagcaacagc gagagaatta gcgattgctg gattcaccac gacgctagtc
    15841 cattttccga catttgccgt tgagatgaaa caggcaatcg gaaaagatca agtggcagaa
    15901 aagttagatg cagtgaaacg ttcacctatt ttgatgatcg atgacattgg tgcagatgcg
    15961 atgagtagtt ggatacgaga cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa
    16021 cagttaccaa ccttcttctc atcgaatttt acaatggatg aattggaaca gcatttgtcc
    16081 gtcactcaac gtggagatga agagccgcta aaagcgaaac ggataatgga acgcatacgt
    16141 tatttgacaa aagaaatcga aatgactggt cgcaaccgac gcaatggttg aacagttgac
    16201 aagtccaact gacatttgac ctagtagtaa aagcaggtca gcgaatcatt atatggacag
    16261 aaatgaccgg agacaatcac cgcaacaagt aatgcagaaa cttaaacgga ggatttttta
    16321 tgagggctat tttttctagt atatctgtag ttggtctatt ttttctttta gcaggctgcg
    16381 gtagcgcaga tagcaacaca ggttctgttg attcgattga tgcgggtcaa acagtaaaaa
    16441 caagcagtac gaaaacggag agacaaagca tatttgtcgg aacgattgaa agtattactc
    16501 aagtagaagc agaagaagaa acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag
    16561 atccagcgaa tatgggaacg tcattcagca atgacggagt gatcttaaat gctactgctg
    16621 aaaaaataag cgtggacata aatgaattga aaatcggtga caaagtccaa tttacattag
    16681 ttgagatgcc gatcatgacg atgtctatcc caccgcaagt tcctggagat tcgattattg
    16741 atgtttcatt gaacaaataa ccaataacat tttttcaaaa ataagaaaat gttgttttat
    16801 agaacgaaaa ggtatatttt ttaaggagat atgggaattt ttttattcct agtctcctta
    16861 ttttatttgg aaaatgctat aattatctag aaggagtgaa gctatgaacc atttagattt
    16921 atctcagcgc ttagccgcag tgggtgactt tgtccctgaa tccgctcgtt tagcagacat
    16981 tggatcagat cacgcttact tacccgttgc attgatatta agaggaaaaa tcgaatatgc
    17041 agttgctggt gaagtcgttc aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga
    17101 tggattagaa gaccgtatca ccgtacgcct tgcaaatggt ctagatgcta tccagccaca
    17161 agatggtatt acagctgtca cgattgcggg tatgggaggt tcactgatcc gagatatcct
    17221 agaagctggc aagttgaatc aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa
    17281 catcggagag cgtacattac gatcttggct gcaagaaaat cagtataaaa tccaatcaga
    17341 aaaaatactt gaagaaaata aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa
    17401 accagttact tacaatgaaa tggagctgat gttcgggcca tttctgcttg aagagaaaag
    17461 tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca
    17521 gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat
    17581 gattgaggag gttttaaagc catgacaatc agcggacgca ctttcatcca aaagtttgaa
    17641 agctattgtc ctcagtggct agctgaagaa ggagacccag tcggactgca tatcggaaca
    17701 ttggataagc caatccaacg agtaatgatg acattagacg ttcgtccaga agtggtcgaa
    17761 gaagcaatca agaaaaaaat cgatttgctg attgctaaac atcctccaat ctttcgtcct
    17821 gtaaaacggc tggttactga tcagccacaa gaaaaaatgt atgcagattt gttgaaacat
    17881 gatattgccg tctatgcagc acacacgaac atggatatta tttgggatgg actgaatgac
    17941 tggttttgtg aacttctcgg tattgaagtg gagagttatt tagtaaagac tcatgagatt
    18001 cattataaaa aactggccgt ttatgtacct gtggatcatg cacagaaaat gcgtgaagta
    18061 ttagctgcag ctggtgctgg tacacaaggt gattataccg gtacaagttt tacctcgatt
    18121 ggacacggtc gatttactcc ggaagctggt gcacaaccag cgattgggaa agtagggaag
    18181 acagaacaag ttcaagaggc aaaagttgag gttattttgc cggaaactat cgaaaaacaa
    18241 gtgattcaag ccatgcggtc agctcatcct tatgaggaac ctgcttatga tcttttcgct
    18301 atagatgaac cagtcgaaat gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt
    18361 tctatagagg catttgtaga acaagtaaaa gaagcgttcc aactagatgg actgagaata
    18421 gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg cgatttgcgg tggaagcggt
    18481 gaaaaatttt atccgcaagc aatcgcccaa agagcagatg tctatatcac gggagatatc
    18541 tattatcata cggctcatga tatgcaaagc gctgggttga ttgcaattga tcccggtcat
    18601 tatattgaat cgttatgcaa gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa
    18661 gaaaattggg atattaactt tttcgtttct gaaacaaata caaacccatt tcaatttaat
    18721 taaaaggaga gaatactatg tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa
    18781 cagaaacacg ctccgatgca acaagcaaaa cgactccatc gactcagaca caagttgctt
    18841 ttgcgcaaga attgaaaaaa gaattagaag agttaggctt agctgatgtt ttctataatg
    18901 aagataacgg attcgttatg ggaacattac caagcaatgt tgatcatgaa gtgcgttcca
    18961 ttggatttat ttcccacatg gatacagcag attttaatgc agtaggagtc aatccacaaa
    19021 tcattgaaaa ctatgatggc gagtctacga tcgtactaga tgcggaagaa cgctatacat
    19081 tgaatacaaa agattttcca aatttgaaaa attatgaagg ccacacattg atcacaacag
    19141 atggcagtac attactggga gcagatgaca aatcagggat tgcggaaatc atgactgcaa
    19201 tggagatctt gatcaataat ccttctatcc ctcacggtac catcaaagtg gcttttggtc
    19261 ctgatgaaga aatcggtgtc ggagcggata aattcgatgt tgagcaattc gatgtagatt
    19321 ttgcttatac agtcgatggt ggtcctttag gtgagttgca gtatgaaacg tttagtgctg
    19381 ctcaagcaaa tattacgatc caagggaaaa atgtacatcc agggacggca aaagatacaa
    19441 tgatcaatgc tctacagtta gcaatcgatt ttcataatca gcttccagcc gatgaagttc
    19501 cagaaaaaac ggaaggatac gaaggtttct tccatttgat ggcattggat gggaatcctg
    19561 aagaagcttc tatgagttat atcattcgcg atcatgatcg tgaaaaattc gaagcacgca
    19621 aagcacaaat cacacatatc caagaagagt tgaatcagcg atttgatcaa gaacgaatca
    19681 aagtagatat gtatgatcaa tattacaaca tgaaagaaat cattgaaaaa gatatgagca
    19741 tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat ctctccagtg atcgaaccag
    19801 ttcgtggagg aacagatggt tcaaaaatct cttacatggg tattccgacg ccaaatctat
    19861 ttgccggagg agaaaatatg cacggacgat tcgaatttgt ttctttacaa gtaatggaaa
    19921 aagcaacaga cgtgattgta aaaattgcag aattgaacac aaagagttaa aatcgaaaaa
    19981 gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag
    20041 ggcaggacaa tggaattggt tgtcccgccc tcttttttga agagtaaata ggaggtaaag
    20101 aatggaaaag ctgctagttt tttataacaa aagttcaggg aaagatgagg gtgagaaatt
    20161 agcacgctgg tttcaagatt atgtaaaaga aaagcagcca gaattatatg tagaactagc
    20221 tgaaactggc ccatcgattg atgatgcgga attagtcaag aaagcagaag ccatagaagc
    20281 tgatacttta gtaattatag gtggagacgg cacgattcat catatcgtcc aagcgttcca
    20341 aaaaacatta tctcaatacc atgtcggatt acttccaggt ggaactgtca acaaccttgc
    20401 acgtgttctt gagattcccc tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa
    20461 tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc attatctcta cactgacaat
    20521 cggaatctta gcagatacag cagtttggat cagtcaagaa gaaaaacagc gttacggctc
    20581 gtggatcttt atcaagaagt tttttaagtt attgctgaag aagaaaaaat atcatttgga
    20641 tttagaaaca gacgaggagc attggaaagg aaaatcccaa ctgttgacag tcacgatgtc
    20701 taactccgtg ggcggtttta caaattttga cgactcagcg accctagatg atggaaaatt
    20761 tcatgtaacg atcgtacctt ccttagatat tttccgattt attttttatt tgcctagaat
    20821 catgagaggc aaattctata cgatccccgg cattacttat ttcacagcca aacgcatgaa
    20881 aatccaagcg caggaaaaat ctgtgggcac tcgaacggat ggagacacga cagatgatct
    20941 tccagttgaa ttatctgttg tatcaaaagg attaacagtt tattcaccaa agaaagaaga
    21001 aaaagcttag aaatagtagt taacagaaag ttggaagaag agtaatggat gtattgttac
    21061 acaatctttt gtggttttac cttttcattg tgaattttta cttattttgt ctgatggggt
    21121 atgataaaca tcaggctaga actagcggct ggcgtgttcc tgagaataat ttgatatttg
    21181 tcagcctgtt aggcggtggt ttgggaggat tgatctctcg aaaagttttc catcataaaa
    21241 ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt acttgttgat ttgattctca
    21301 tttatttttt ccattaagga gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt
    21361 ctgaatctgc ttttgctgat tgtgattttt gccattattt attatttaat caatcaatca
    21421 tttgcagata tctttgccga attgctgtct accagtgttg ttgttttggc agcggctgtt
    21481 atattaggga cagtttacca aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct
    21541 ttcgcgccga attttacaac gaaagatggg ttcttgacct ctagctatat tgcgttttac
    21601 cgaatcgttt cgtttggtac aggaacgctg atctcggaga tttattttta taacaaaaaa
    21661 ggaatgaagc tatcccaggg agcaggggtg acagcactcc atatgattat gtacaaactg
    21721 gcagtgatca cttatgcatt agctggcttg gtcatccaat tctctctttt ttattctaat
    21781 ggtccatcaa tgatctggtt tatcatcgct ggtatccttt tgacgatcgt gattgttgcc
    21841 tttttattgg cgatgtccgt cagcttgaat gttcaaatat tttttgtcag cttatcgaat
    21901 aagttattta aaagtgagcg tttgcggaaa tgggtggata catgcaatca gcagatctac
    21961 tctttaaggg aaacggtcca aaccattatc caagacagga ctgcgctatt gcggatttat
    22021 ctatggaata tgttaaaact tgtattttgg tatgtacttc cttatttgat tctgattgaa
    22081 aaccatccta aaatagattt tctactgacc atttcgttta tcagctttgc agtagtgctt
    22141 tcaggggtta ttcctacgcc tgctggtatt ggttcctttg aatttgtcta tttgttatta
    22201 ttcagaccag ttgttggaac agttgacgca gtttcttcgc ttttactgta tcgtttcagt
    22261 tcctttatct tgccatttct ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg
    22321 atcaaagaag aaataataga agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt
    22381 tataagatct acccttttta tcaaatcaaa gaaaaacgtt atggataaga taattttacg
    22441 tttgtttggc tcttttttta tatacataca atcaattttg tttttttaag atttatataa
    22501 gtcgagaaaa aaagtcaaaa aaccatgtgt atattttgac caaaattgac caaagcgata
    22561 taatagtttt tgtaaagatc aaagaaataa gtgatcttac ctttaattag gagggtatac
    22621 ctgtatgaat attgagaaaa tgactacgac gcttcaagaa gcgattgctg aagctcaaca
    22681 gattgcggtc actcgaaaac atcaagatat cgacattgcc catgtatgga aaatcttttt
    22741 acaacctaat cattttggtc gaaatttcta tacggatgcc ggattggatg tcgaatcttt
    22801 tgaacatgaa attgatcgtc tactggatga gtatccagtt gtttctggtg gaaacgtcca
    22861 atatggacaa aacctcagcc agaatttctt tcgattattg aatgaagcag accaaatacg
    22921 tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt
    22981 aaaaaattat ccgttaactg tttatttgaa taaaaacgga ttatctgaaa aagaattgcg
    23041 taaaaacatc gaagagatga gaggaggaga aagagtgacc tcacaaaacc aagaagaaca
    23101 atacaaagca ttagaaaaat acggcgttga tttagtacag caagtcaaaa gcggaaaaat
    23161 ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa
    23221 aacaaaaaat aatcctgttc tgataggaga acccggtgta gggaaaacgg caatcgttga
    23281 agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat
    23341 tttttcatta gatatgggtg cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga
    23401 aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat
    23461 cgatgaaatc cacaatatcg taggggcagg aaaaacagaa ggaagtatgg atgccggaaa
    23521 tctgttgaaa ccaatgcttg ctcgcggtga attacatttg atcggtgcca caactttaga
    23581 tgagtaccgt caatatatgg aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt
    23641 agtcaaagaa ccaacagtgg aagatacgat cagtattctg cgtggattaa aagaacgttt
    23701 tgagatccac catggtgtta acatccatga caacgcatta gtagcagcag ctactttatc
    23761 tgaccgttat attactgatc ggttcttacc ggacaaagca atcgatttga tcgatgaagc
    23821 cagtgcaacg attcgggtag aaatgaactc tatgccaacg gaattagatc aagttactag
    23881 acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag
    23941 taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc
    24001 gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc
    24061 tgaaatcgat aaagcaaaac acgaattaga agatgcagaa aataactatg atttagaacg
    24121 cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa aaagaattga aggaattaga
    24181 agctaaagca aaagacagcg agattaagat ggttcaagag tccgttaccg aaaatgagat
    24241 tgctcaagtc gttggcagat taacgggtat ccccgtaacg aaacttgtag aaggtgaacg
    24301 agaaaaacta attaaattga atgaaacact tcataaacga gtgattggtc aagatgaagc
    24361 agttgatgcg gtcagtgacg cggtaattcg ttcacgggct ggattgcaag acccaaatcg
    24421 tcctctaggt tctttccttt tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa
    24481 agcgttagct gagaacttgt ttgactcaga agatcatatg gtacggatcg atatgagtga
    24541 atatatggaa aaacatgctg tgtctcgtct agtcggagct cctccaggct atgtaggtta
    24601 cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt
    24661 ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc ttgttacaag tcctagatga
    24721 cggacgcttg actgattcaa aagggcgagt agtagatttc aaaaatacgg ttctgatcat
    24781 gaccagcaat atcggttcac agttactgct ggaaggcgtg actgctgatg gaacgattcc
    24841 agaagcagtg gctgaacaag tcaacacgct attacgtgga aacttcaaac cagaattctt
    24901 gaaccgaatc gacgacacga ttttatttac accattgagc ctagacaacg tgaaaggaat
    24961 cgttgacaaa atggtggcgc aattggcaca acgtctggaa catcaagaaa tcctgctgac
    25021 aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct tatgaaccag catatggtgc
    25081 aagaccattg aaacgattta tcactaagga agtagaaaca ccactagcta aagaaatcgt
    25141 tgcaggacat gttatgccta aatcaaaagt aacgattact ttacttgatg gacaacttca
    25201 tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc attgagacta taaaag
//
LOCUS       DACBZN010000032        24331 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.032, whole genome shotgun sequence.
ACCESSION   DACBZN010000032 DACBZN010000000
VERSION     DACBZN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.032"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(473..1060)
                     /locus_tag="IVD64_001290"
     CDS             complement(473..1060)
                     /locus_tag="IVD64_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148550.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1235..2557
                     /locus_tag="IVD64_001291"
     CDS             1235..2557
                     /locus_tag="IVD64_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6148551.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2712..3488
                     /locus_tag="IVD64_001292"
     CDS             2712..3488
                     /locus_tag="IVD64_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6148552.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3481..4050
                     /gene="rnmV"
                     /locus_tag="IVD64_001293"
     CDS             3481..4050
                     /gene="rnmV"
                     /locus_tag="IVD64_001293"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP6148553.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4043..4927
                     /gene="rsmA"
                     /locus_tag="IVD64_001294"
     CDS             4043..4927
                     /gene="rsmA"
                     /locus_tag="IVD64_001294"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP6148554.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5378..6007
                     /locus_tag="IVD64_001295"
     CDS             5378..6007
                     /locus_tag="IVD64_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148555.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6131..7240)
                     /gene="ugpC"
                     /locus_tag="IVD64_001296"
     CDS             complement(6131..7240)
                     /gene="ugpC"
                     /locus_tag="IVD64_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP6148556.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7449..7871
                     /gene="mgsA"
                     /locus_tag="IVD64_001297"
     CDS             7449..7871
                     /gene="mgsA"
                     /locus_tag="IVD64_001297"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP6148557.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8194..8730
                     /locus_tag="IVD64_001298"
     CDS             8194..8730
                     /locus_tag="IVD64_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6148558.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8770..10533)
                     /locus_tag="IVD64_001299"
     CDS             complement(8770..10533)
                     /locus_tag="IVD64_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148559.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10536..12251)
                     /locus_tag="IVD64_001300"
     CDS             complement(10536..12251)
                     /locus_tag="IVD64_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148560.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(13030..13254)
                     /locus_tag="IVD64_001301"
     CDS             complement(13030..13254)
                     /locus_tag="IVD64_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148561.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13454..16222
                     /locus_tag="IVD64_001302"
     CDS             13454..16222
                     /locus_tag="IVD64_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6148562.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            16303..16722
                     /locus_tag="IVD64_001303"
     CDS             16303..16722
                     /locus_tag="IVD64_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP6148563.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            16734..17204
                     /locus_tag="IVD64_001304"
     CDS             16734..17204
                     /locus_tag="IVD64_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6148564.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            17221..18000
                     /locus_tag="IVD64_001305"
     CDS             17221..18000
                     /locus_tag="IVD64_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6148565.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            17987..18808
                     /locus_tag="IVD64_001306"
     CDS             17987..18808
                     /locus_tag="IVD64_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6148566.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            18825..19874
                     /locus_tag="IVD64_001307"
     CDS             18825..19874
                     /locus_tag="IVD64_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6148567.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYIQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            19887..20891
                     /locus_tag="IVD64_001308"
     CDS             19887..20891
                     /locus_tag="IVD64_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6148568.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            21524..22543
                     /locus_tag="IVD64_001309"
     CDS             21524..22543
                     /locus_tag="IVD64_001309"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6148569.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            22586..23467
                     /gene="galU"
                     /locus_tag="IVD64_001310"
     CDS             22586..23467
                     /gene="galU"
                     /locus_tag="IVD64_001310"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6148570.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            23767..24144
                     /locus_tag="IVD64_001311"
     CDS             23767..24144
                     /locus_tag="IVD64_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148571.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT         7920 a         4201 c         5006 g         7204 t
ORIGIN      
        1 aaagggggta tgacactagt tgtgtcatac cccctttttc cgaaatttga gaagcaattt
       61 tcgtaaattc cttcttatta cttgaagcca atcatataat acagaaacag agaactatcc
      121 aaaataaatc ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt tagaataata
      181 ctaataggaa tcgtaaaaac agaaaaaaag cataatgata aagggtcggg cagattttaa
      241 ttgattcatc tggtactctt ttttattgtc cttacattca taaagtttga atgcaatgac
      301 tttattacat ctatatttat atctaacagt agcgttgatg tgttaattat aagttctatc
      361 actttatgtt caattagttt gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac
      421 aatcatcggc tttgacttcg atgattgttt tctttatttt gtaaaaatcc tatcacttga
      481 ttttatggta accttttatt tttgtcttca aacggcttgg taagagagca gtaagcagtg
      541 caaatgggat cgaaaagaca taccagaaaa tcgtacgtcc gataccataa tgttgataga
      601 gctttgtcaa catcacgtgt aacaagatca accctaaaag caaggagcca gctataaacc
      661 aatgattatc ccaatgcatg acttcactaa gtgccatcac tccaaaagga tagaaataaa
      721 aatagatccc gactaaggtc atccgccagt tctttgtaaa tgttccgata ttcaataaaa
      781 agaaaatcgc tgaaagcaaa ataccgatag gcggaaaaag cccaaccaaa aaggaataca
      841 acatccccca caagatcatc acaggacctt tgatcaatgc aacgattgcg atgaacaaag
      901 tcagcaatat tagtttttgt gataacgttg caacagtcat taatccaaat aaaattaatg
      961 tgaaccaatg ccaatgggac tgctgttgtt ctttacggga aaaatcgacc cggttggata
     1021 aataatctgc taatttcgga taagaacgct taaaatccat aaactgttcc tccttcgtgg
     1081 aaaaggtagg tttagtatac gaaaaataaa aaagaaatac atccgtctta agagggagtt
     1141 tttcactgaa agtggagaaa aaagaatggt aaattaaaat attctcagtt atactgaaaa
     1201 ttataccaaa tttcaaagaa gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat
     1261 caatctgttt ggcgcattag ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt
     1321 caaaactgct gctgttactg ctagtactca gtctacaagt ttgacattgc tagcttggct
     1381 tttgggtggt ttcttaacga tctgtgctgg attgacagtg gctgaattag ctacagcaat
     1441 cccggaaact ggtggagctg taaaatatat tgaagctgcg tatggaaaat tgcccagctt
     1501 tttattagga tgggcgcaaa gcttgattta ctttcctgct aatattgctg cattatcgat
     1561 tatctttgcg acacaattga cgaacttatt acaattatct accgattatc ttttgttgat
     1621 tgcagtgatc accgctgttt ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc
     1681 ttctgtacaa tcagtgacgt tgattgttaa attaatacca attgctgtga ttgtcatatg
     1741 gggattattg accccaggac aaggaactgt ccagttattt ccaatcgaag ctggaaaaga
     1801 tgttactttt gtcgaaggat tgagcagtgc gttgttagca acgttgtttg cttatgacgg
     1861 ctggttaggt gtcggtgcaa tggcagggga aatgaaacgg ccggaaaaag atttgcctaa
     1921 agcaattatt cttggtttga gttttgtaac agtcgtttat ctattgatca attttgtttt
     1981 tttgaaaact ttgccgattg atcatcttgc cggtaatttg aatgcagctt ctgaagcatc
     2041 agacgtcatt tttggaggaa tcggtggaaa gctggtcact attgggatct tgatctcggt
     2101 atacggtgca ttgaacggtt atacactaac tgggatccgc gtgccttatg cgatggcttt
     2161 agaagacgag ctacctttca gtaaacagct gacaaagctt tcaaagaagt ttacggttcc
     2221 ttatgttcct gcggttttcc agttagcagt agcttgtatt atgatgagcc ttggttcttt
     2281 cgacttttta acagacatgt tgatttttgt gatgtggcta ttcagtttac tgatttttat
     2341 cggtgtcttt attttaagaa aaaaagcgcc ggaattgcca cgtccatata aagtaccact
     2401 ttatccaatc gttccgatta ttgcgattct cggtgctatt ttcattttag gtatgacgat
     2461 gatgacgcaa acgaaattag cattgatcgg tataggtgtt accttgatag gtatcccagt
     2521 ctattatcaa aaaaagaaaa aacgctcaga agagtaaaat atttcaacaa cagacagaaa
     2581 aagtacgaac taggtcctct agatcttcgt gctttttcta tttcaataaa acaagttctt
     2641 ctattttgat ttttgctact tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag
     2701 gagagataaa catgatattt gattctcata cccatttaaa tgctgaacag ttcaatgaag
     2761 atattccaga aacgattcag cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg
     2821 gcttcgatac gccaacgatt gaaaagtctt tgcaattaag ccatgactat ccaaatatat
     2881 acagtattat cggctggcat ccgacagagg ctggcagtta tacgaaagaa atcgaaaata
     2941 aattacaaga acaattgact ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt
     3001 atcattggat ggaagacccg aaagaagtcc aagataaagt atttcgcaga cagattgcta
     3061 tcgcaaaaga aatgaaccta ccaatcagta ttcatactag agaagcactt gaagacacgt
     3121 atcaaatcct aaaagaagaa gatgttcgag atatcggtgg aatcatgcat agtttcagtg
     3181 gtgattatga atgggcaaaa cgatttcttg acttagggat gcatatttca tttagcggtg
     3241 tcgtcacttt caaaaaagca ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac
     3301 gattacttgt ggaaacagat gccccttatc tggcaccagt tccttttaga ggaaagcgaa
     3361 atgaaccagg ctatactcgt tatacagtag aaaagattgc tgaacttcgg aatcttccga
     3421 cagaagaagt ggctgcccaa acatggacaa atgcgcaccg attatttggt ctaagcgaac
     3481 atggataaga tgaagatcca agagattata gtcgttgaag gaaaagacga tacaagaagg
     3541 ctgcgggaag tagttgatgc agatacgata gaaacaatag ggtctgcaat caatgaagag
     3601 atcctgatgc aaattgaaca tgcccaagag actcgtggag tgatcgtttt taccgatcct
     3661 gatttttcag gagaaaaaat aagaaaaatc attatggaag ctgttcctca ggcaaaacat
     3721 gcttttttgc cgcgcacaca agctaaacca aagaaaaagg gaagccttgg tgtcgagcat
     3781 gcaagtgatg ctgctatttt agaagcattg aaaaaggtcg tgacgccaga catttcccaa
     3841 acggctgttt ctgaaatcac tagacaagac cttttagact atggtttaat agctggggca
     3901 caagcaaaag aaaaacgaga aaaactaggt gacgagttgc gcatcggtta cacgaacggc
     3961 aaacaattgg aaaaacgttt gaagatgttt cgtattacgc cgaaagaact cgcccaagca
     4021 atgaaaatag tggaggaaca acatgagtga atatcgtgaa attgcgacac cctctagaac
     4081 aaaagagatt ttattgaaac atggtttttc atttaaaaaa agtttaggac aaaattttct
     4141 gactgaacca aatattttaa ggaaaatcgt agaaactgcg caaatagatg aacatacgaa
     4201 cgtgatcgaa gttggaccag gaatcggtgc attgacggaa caattagcca agcatgcaaa
     4261 acaagtcgta gcatttgaga tcgatgatcg tttgatccct gtattggctg atacgatgca
     4321 accctatgac aatgtgacta ttgtacatca agatatttta aagaccgatt tgtcaacagc
     4381 tgttagagaa acatttcatg aggagcttcc gttaaaagtc gtagcgaatt taccttacta
     4441 tattacgaca ccgatcatga tgcattttct tgaatcagat ttggttgtgg atgaactagt
     4501 cgtcatgatg caaaaagaag tagctgatcg tatctctgct gaaccaggaa cgaaagctta
     4561 tggttcatta tcgattgctg ttcaatatta tatggaagcc agtttagcat tcatcgtacc
     4621 aaaaacagta tttgtccctc agccaaatgt agattcagcg attttgaaac tgacaagaag
     4681 agatataccg gcagtcgaag tgacagacga aaaagcattt ttccgattga caaaagcggc
     4741 atttcagcaa agaagaaaaa cgctttggaa taacttgcag cattcgtatg gcaaagatga
     4801 ccagacaaaa gcatggttag caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg
     4861 tggggagact ttgtcattac aagaattcgc cgcattaagc aatgcgatgt cagaaaatat
     4921 gcagtaaaaa aagaggttgt gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa
     4981 gaacagccaa acaaaaatag ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga
     5041 tgcaagcttt tgaacaccgt ttaagaaaga ggttgtgggc ctgccgttta actctaagtc
     5101 acaaggaaca atttcaaaaa acagcttctc atgtttttga gttagttggt cttattactt
     5161 gatgcaagct tttgaccact attatgccga acaaggagat gtgacacaaa ttttgtcaca
     5221 tctcctattt ttgtttcttg atcaaaaaat tcttatactt gatactcaca ctcattttgt
     5281 atggatttag aaaagaatat gtgtcttgac agttattagt aagtgagtta tactaagtcg
     5341 acatttatgt gtcaagacat attaggagag agaaaaaatg agaaaaaatt cagttcgaaa
     5401 actaacgatt tctgctttat tgattgggat gggggtcatt atcccaatgg tcatgcctaa
     5461 aatcatgatt ccaccagctt cctttacttt ggcgtctcat gtgccattat ttattgcgat
     5521 gttttttaca ccaggagttg cagtggctgt tgcgctaggt acgacatttg gtttcttttt
     5581 aacaacgccg gttatcatcg cattgcgagc attgtcacat cttgtgtttg cgttgattgg
     5641 ggcttggtac ttgcaaaagc atccgagtat cgttttaaaa aatggacagt ttacttttgc
     5701 taattggcgt ttccaagggt tcaattttgt gatcggtgtg attcattctt tagcagaaat
     5761 gctagtagtc agcatctttt actttattgg taatatgcct gaaacatatt attctcaagg
     5821 atatttttat acagtattca tcttgatggg gattggtggg cttatccaca gtttagttga
     5881 ttataatatt gcttatttta tagcaggaac actcagcaaa tattttgata tccccgtatt
     5941 tacggcagca aaaaaagaaa atcggaaaga gtcgattcaa gggtacgcga agaaaacgac
     6001 atattagaca tgatctctca aaaaaaaaac tctcatctag taatggataa taaatcctct
     6061 aagatgagag ttttttgtcc taaaaagagc tgcaccaaca tcttctggca cagctctaac
     6121 agggggcaat ttatggaagt ttgatgactt gttctgtttc tttatcaaag aagtggcctt
     6181 tgctgatgtt gaaggcaagg tcgactattt cgcctggttt atggaaatct cttgcatcta
     6241 cttttgagat gaactcagta gaaccagttt ttgtgtaaag catcgtttca gctccaagta
     6301 attcggaaac gacaacttct gatttcacta cggcttcagg tgaagcttca agggctactt
     6361 gttcactgtg gatgtcttct ggacgaatac cgaagataac ttcttttcct tcataacctt
     6421 tttcaactaa tactttgttt tttccttcag gaatacgaag cttaagccca tgtccatctg
     6481 tgataacacc atttttcaat gtcacattaa agaagttcat ggcaggagat ccaataaagc
     6541 cggcaacgaa catattgacg ggtgtatcat aaacttcttt cggtgtaccg atttgttgga
     6601 taaatccgtc tttcatgata acgattcggt cagccatagt cattgcttct gtctgatcgt
     6661 gagtaacata gatggtcgtt gtttcaagac gctggtgaag tttggcaatc tcagcacgca
     6721 tggctacacg taatttagca tctaaatttg ataatggctc atccattaag aagacttttg
     6781 catcccgaac gattgcccgt cccaatgcca cacgttggcg ttgtccacca gataaagcag
     6841 ctggttttcg tttcaagtat tctgtcagac ctaagatctc tgctgcattg tccacacgtt
     6901 ttttgatgtc tgctttatca tatttacgta atttcaagcc aaaagccata ttatcaaaaa
     6961 cagtcatatg aggatacaat gcgtagtttt ggaaaaccat ggcaatatca cggtctttag
     7021 gtgccacgtc attcatgact tttccgccga tatttagttc tccttcagag atatcttcca
     7081 gtcccgcgat cattcgcaaa gtagttgact ttccacaacc ggaaggacca acaaaaacga
     7141 taaattcacg gtctttgatt gatagattaa aatcagttac agaataattt tctgcattgt
     7201 catatttttt atatacgttt ttcagagcca tttctaccat aatacgttcc acactttctt
     7261 ttttagtttt attaactatt taaagtataa atgaaagcgt taactaactt caatgtcatg
     7321 ttgaacaaga attttttctt ttttttgtca atgtgctagt tttaagcttt aataaatatc
     7381 cataagaaat aaaaactgat acactggagt aaataagtat gttaacaata tcaaaaggag
     7441 tgtctcttat gaaaatcgct ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat
     7501 tagcaacagc gtataaaaca atattggaaa aacatgaact ttttgctacc gggacgactg
     7561 ggcagaggat cacggaagca accggcttgc cgatccatcg gtttaaatcc ggaccgttag
     7621 gcggggatca gcaaattgga gccatgattt cggaggatca gttagatttg gtgatttttc
     7681 ttcgagaccc gctggctgca cagccccatg aaccagatgt aacagcactt atccgtttat
     7741 ctgatgtcta tgagattccg ttagctacga atatcggaac agctgaggtg ctactaagag
     7801 gactggaagc cggcttccta gattttcgaa gaatcgtcca tgaaatagac aagcatccac
     7861 tttcattttg atggataagc gtatggaaaa ataacgttta atgttcgcct atatgtagct
     7921 ataaaagcta aacaaactga taatatgcga tataatacga acgtattgga taatataaac
     7981 aataatatta gtttttcgta tttgcagtaa atgtgaaaat atgctatagt ttctgtggga
     8041 aaagatccca aaaataatac agagtagaaa ataaagcgtt aagtgctaag aggatgggat
     8101 gttgcctctt ggacgaagga cgtaatgttc gcggttttat tttcgcattc gctgcatgat
     8161 acgatgtagc agctcttaaa ggagatgcta aaaatgaaga aacaccggtt agacgcacgt
     8221 atgattgcta ttatgggctt attgattgca ttgatggtca ctttgtcccg actggtagca
     8281 atcgagaccc catttattaa aatcagtgtc acatttattc cacaagtgat catgggcatt
     8341 ctttttggtc ctttttggtc agggataggc gcagttttag ctgatttagt aggaatggcc
     8401 ctcttctcga aatctgcatt ctttattgga tttacattga atgcatttat tgaaggagca
     8461 atctacggat tctttttcta tcgaaaagag atcacttgga aaaatgccat tctggctacg
     8521 cttagtgtca cgctgatcat caacttattt ttgacaccat tatggcttgc gttgatgtac
     8581 catgtgccat tgttcagctg ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg
     8641 ttaccgattc aatcaattgc catttattac gtaggacgta gtattcctta caaaaaaatt
     8701 ttaagaagtt tagcgatcca cgctaaataa aaaacaagca gtcttcccat cccagaagac
     8761 tgcttgtttt tattgtgctg tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt
     8821 ttgaagcagc tcatcatggg tgcccatttc gacaatagct ccctgatcca tcactaatat
     8881 ctggtccgca tttttgattg tggacaggcg atgggcaatc acaaaactgg tttttccttc
     8941 catcattgct aagaaggctt gttggatttt tttctctgtc aatgtatcga ctgagctggt
     9001 agcttcatct aagatcaaca tagggggatt actgatcatc gttcgagcaa tcgtcaacag
     9061 ttggcgttgt ccatctgata tctttaaccc ttgaccgcca atcttagtat ctaatttttc
     9121 cggcaaacgt tctacaaatt catacatata agaggctttt agggcatcat tgatttctgc
     9181 gtcacttgct tccggattcc caaaacgaag attttctcgc aatgtactgt caaatagcca
     9241 agtatcttgt aacaccattc caaaactttt acgtaatgta tcacgttgga tagagcggat
     9301 gtcatggtca tcgactgtaa ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt
     9361 aacaagtgtg gattttccgg cccctgtttt tcctactata gcaatggttt gacctggata
     9421 agcagtaaaa ttgaaatctt cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg
     9481 atcaaaagag acttttcctt tgatattttt gggttccaag gcataggcat ctggcaaatc
     9541 aggcttttct tctggctgat cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt
     9601 ttgcagttgt gtcgtgattc cagaaagttc gataaatggt ttagaaaact gacttgagta
     9661 gatcgtaaag ctggaaatca caccaacagt gattgacgaa ccactcttca atgccaatag
     9721 gccgccaact aaaccaatcg ataaataagc aaggtgatct acaaaacgag aagacgggtt
     9781 tgtcaaggaa gaagaaaatt gtgcccgttg tcctttttca tatagatcag catttagcag
     9841 ttcaaattgc tgttgactag cttcttcttg ctgaaaagct ttcacgattt tctggttacc
     9901 gattcgttcg gtcacaaagc ccgagatatc cccaataatc ctttgctgtc tatcgaaatt
     9961 tgtttgtgaa gcacgagcaa cgatccaact cacaaggaaa ataattggtg tagaacaaag
    10021 aacgaccaat gtcagaagtg gacttagacg catcataaaa ataaacgaaa gaatgatgat
    10081 tgccactcca gaaaataatt gattgaaggc cgcagaaatt gcaatggaaa tattgtccat
    10141 atcatttgta aatcgactga cgatatttcc gtgggagttt tgatcataat aacgaagtgg
    10201 caatcgattc agatgagcaa atgcatcttt tcttaatttt gcaacggata agtaagctaa
    10261 ccgattgctt aacacttgga tgagccattg actgactacg ttgattaaaa cgataccacc
    10321 gaatagaata agtaaatgga acagtcgatt gaagcggacc tgtccttctc caatcatttg
    10381 atcgatactt tgaccaattt gtaacgtcat cacaactgtt gcaatcccat tgatgattcc
    10441 tagtatcaca gctagccaaa tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg
    10501 gagcgtctgc aaggataatt tttttacttt cataactatt cttcctcctg tgatgcgacg
    10561 atttcctgat attctgctga ttgagcaagt aattcttcat gggtaccctg agcagcaatc
    10621 ttaccttgat ccatcactaa gatatgatgt gcttcttgaa tagagcggac acgttgggag
    10681 ataataatca ctgtactgcc aagataagtc tgtagcgcct tacgcaaatc caagtctgtt
    10741 tgataatcca acgcacttaa agagtcatct aaaatcagta atgctggctt ggcaatcaaa
    10801 gctcttgcaa tcgtcagtcg ctgtttttgt ccgccagaga aatttttccc attttccatg
    10861 acttttgtat ctaaaccatt tggaagctga cggacaaatt cttctgcctg agcgattttc
    10921 agagcttccc aacattcttt ttctgtggcg tctttcttac cccattgaag gttttcacga
    10981 atagtcccgg taaatagtac agaagtttgg ggaacaagag caatttgctg acggagatga
    11041 tccagctgcc aagaacgaac atccattccg tccattataa ctgtaccgga actgacatca
    11101 tagaaacgcg gaatcaattg tgtcaaagtc gttttcccgc ttccggttgg accaatgatt
    11161 ccaaggactg tatttcgctt cagctgaaat gtaatatttt ggagagaaag accagaatca
    11221 ggagtataac ggaagtcaac atggtcaaaa gcgataattg cttcagaaga aaaatcggga
    11281 gtagtactga cctcagcttc aagaatcgaa ttttctgtgt ctagcacctc tttcacccga
    11341 tttcctgaag cttcggcacg tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc
    11401 aaaagcattt gattcatata attgatcaaa gcaagaactt gtccttgttg caagctacca
    11461 atatttactt tgatcccgcc aactgtcagc aaacagatga tcccgacatt catgatcaag
    11521 gttgtagcag gtgataatag ggcagagata ttggaaacac gtaaataatt gttcgctaga
    11581 tcatttgtgc ttttatcgaa cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg
    11641 acacgaacac cgctaagatt ctgactaacg tgttcattga gctgatctag tttttcttgc
    11701 actttttggt agagaggaac ggtttttttg atgataaaaa ataaaatgat tgagaaaata
    11761 ggcaataatg ctaagaaaaa cagacctact tcccagtcga tcacaaaagc catgaccact
    11821 gacccgatac ttaaaaacgg tgctctgatc aataaacgaa tgaccattgc taaagctagc
    11881 tgaagctgat tgatgtcgtt tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc
    11941 aactcgttgt gagagaaagt attgattttt ttgattagct gattacgtaa ctctgtacca
    12001 aatccttgtg aagctactga agcatagtat tggcaaatga tagcacaaat cagaccaatc
    12061 acagacatag cgaccatcca taagcccatt tcaatgattt ttcctcggtc attcattttt
    12121 agtccattat cgatcaagga agccatcatt aaaggcagga caagttcgaa gacagcttca
    12181 agaaatttga agaacgggcc caaaatgatt tgcttacgat agtcttttgc ataacgtaat
    12241 aactgaatca tatgattcct cctattattt atttgtccac aattgaaaaa acaagtacta
    12301 ccacattgta gcttaggaaa tttagaatga aaagagaaca agccttttca taaatgaaag
    12361 ttttattatg aagaagcttt agaaattgaa tagactattt tttttaaaaa aaagaaaatg
    12421 agaaaacatg ccagtctctt cattcatagg aacgtctatt ttacttaatt tttctataaa
    12481 tgaattctgc ataaaaaata tatcaagtaa atgtttatag atgaaactct atttacttct
    12541 tttttaattt gttcttgcaa tcttttttgc tacgtaattt ttgtaaaaaa gtgggttgag
    12601 agaaatctaa ttgattttca aagaaggagt atgaaaatga gaaaaatatt tattcgttta
    12661 ttgtgggtac tatcttagag gaataaataa tagtggagaa aactatggtt tggcgcagat
    12721 tctatcaggt ttgatggaac gaagacaacc tcccgacatc aagtcaaaag ctacttgaga
    12781 ttttgatctg aaaataaaaa aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt
    12841 tcgtaatcaa tgggaaaaaa ctaagagtgg tgtacgtaat caataaaaag gatggtgaca
    12901 acagtacctc tttactctct gaaagcatca taactcgttt ttcctcatta tgccaataaa
    12961 ttgttgttat accacatata aggatttgta atagctaaaa acagacagga aagttgaaat
    13021 ctgtgaatct tattttgtat ggaaggattt atagaacgca aaaatcaaga taaagatgaa
    13081 taaaaatcct gtttcggcta tatattgcgt atctacgcca ataaaacgag tttgttctgt
    13141 catagccata taaagcaaag gcaaaaatac aactacagcg agataagatc cgatgatttc
    13201 tcgaaaagtt ttccaggaag tatgtttcct gtttttatat ttatccgatt tcattgaata
    13261 gtcatagcct ccttattctt tcatatgttg aaattccagt ataaaaactg gaaggaactg
    13321 tacgtttttt agaataaaaa ttataaggga taagtaggct gctgtctata ataagatata
    13381 tttttcaatt ctttattaaa gattttattg acaaaatgaa tccgttttct atgatggagg
    13441 aggggtgaat aagttgttga agaaggaaat tttatgttac ttagagaacc agacagcctt
    13501 tttcgattta gaacatatga acgaggtctt tacagcacaa aggttagctg atcattttgg
    13561 ggtaaaaaga aacacagtca gtcattattt gaaccagtta aatgatgagc aactactagt
    13621 caaaatcaat tctcgaccag tgatttattt ccacaaagaa gcttttgaaa aacaattttt
    13681 taagttacgc acgaattttt atttttctat cgatgatctc aaagaggaac agcctttttt
    13741 cgcgcaacca aaagacttat tctcttttat gatcggatac gacgccagtt tgaaagaaag
    13801 tatcgaacaa atcaaaacag ctctttacta tccagatggc ggattaccac ttttgattac
    13861 aggcgaaagt ggcacaggta aaagttattt agttaatttg gtctatcagt attgtctgct
    13921 tcatgatctg ctagaagatt ctgcaccatt catcactttt aattgtgcac aatatgcaga
    13981 taatcctgaa cttttgacta gcaatttgtt tggacatgtt aagggagcgt acactggtgc
    14041 agaagaaaat aaaaaagggg cttttgaagc agcagatggt gggatcttgt ttttagacga
    14101 ggtacatcgc ttaacaccag aaggtcagga aaaactgttc acctatcttg atcaaggtgt
    14161 gatttatcgt atgggagaac ctaatttgtc acgccggatc aaaacacgtc tcttttttgc
    14221 tacgactgaa gagattacta gtcatttctt aacaactttc attcgaagaa tacctatccg
    14281 aatcgaacta cctgcactta atcagcgtag tcgtaatgaa cggctagaac tgatttactc
    14341 tttttttctc gaagaacaaa gaaaaatggg gagccctttg tcagtcagtg gaccagcttt
    14401 gtcactttta acaagtcaga aattatccgg aaacatagga gagctgaaaa atatcgtcaa
    14461 agtttcagca gcaaaagcat atgctgaaca gcgtgaacag cctgaaatcc atgtcacgat
    14521 ccatcatttg caaaaagaac tacttgcgca gcctattact aaaaatacag cacagcaagg
    14581 aatctatatt acgcaagacc agaccttaga gcaattaatg gagaagcaac agcccgaaca
    14641 gcagcgaatc gttaaaagtt ttgagcaaat cctaattgat ttcaagaaaa ataactgtct
    14701 gctatattct tgcgaaggaa aattaaagca agaagtgatt caattatttg attttttgct
    14761 gtttgagacc agtagacaag aaaaacacga gctgctggtt tattggacac aatctatccg
    14821 agagaccttt cgacaaatgg aaactgccta tcaaatcaaa ttcgatggaa acagtgtcta
    14881 tgccttgagt tactatctct atcagcgaag cactatcaaa tggttaccgg aagaacatga
    14941 tattatccaa ctaatcaaag aattagaaca acaagtttct cagtcttatc catctagtta
    15001 tcactatgtc caacgaattc tggaattaag taagatcaaa ttagatattg aagacacaag
    15061 tatggatcgg attttactga cgatctatct aaaaaaagcg aaatggtcaa aagaaaaacg
    15121 actgccaaaa gcgatgattg ctgctcacgg ttatgcaact gctagcagta tggcaaatgt
    15181 agtaaatcgt ttattacaag aggatctatt tgaatcattt gatatgccac tagatgtcac
    15241 accacagcag attgcttcag aaatccttga ttattgcgaa aacagcgatg tttcaaatgg
    15301 attgatcata ttagtagaca tgggatctct gaaagaaatc catcagtttt ttaaaaaaca
    15361 gttatccgta ccgcttttga ttttgaataa tgtgacgaca cctttagcaa tcacagtagg
    15421 ggaatgcctg caaaaaaatg tatcattaga agagatagca gaagagactg ttcgacagat
    15481 ccagccagaa tggcgactgc tctatccaga ggaaaacaaa cccaaagcac tcattactac
    15541 ttgcttcact ggaatcggta cggcggtcca cttaagtgaa ctgctagaaa aaagcttacc
    15601 tacggcttgt cagttgaaaa tcatcccata tgaatatcag caattaaaag ataagaaaaa
    15661 cagtgaccca ttattttcca tatacgaagt cgtaggaatg attggcacca cagatcctga
    15721 tatcaccaat atcgactatc tttcattaga agacctcatc tcgggggaaa agatgtctgt
    15781 actagctgaa tggttggaca ctactatgaa tgcttcagag aaggaaatat tcaatcagcg
    15841 catcattcgt aatttttcat tggaaaaagt actggattcc gtgactattc tagatacaga
    15901 gaaggtgatg ggagaaatcg atcattttat gagagaatta gaggtgcagt tgaatcgaag
    15961 catcagcaat cccaaaaaat tagcgttgta tgtccatgtg agctgtctca ttgaacgatt
    16021 gatccgacaa atgccaatcg aaacctatca aggattagag aaatggaagc agtgtcaaaa
    16081 agaagggtta tctgctatta aatctgcgtt tagtgtcatt gaagagcatt atagtgtcat
    16141 gatccccaat tctgagattg cctatatcta tgatattttg agtgaagata cagagtttct
    16201 ctcgatagaa gaagaatttt gaacataagc aagttgttgc cactaatttt ggcatgcaac
    16261 ttgctttttt agtatgtgta agtgaaaaga ggagatgaaa aagtggatag agaaattatc
    16321 ttagcttccc atggaaaatt tgcttcaggg atattagatt cattagagct gatctatggg
    16381 aaaaatcatt ctattactgt attcgattgc tatactgatg agaattttga tttagcggaa
    16441 acagtaaggc agctttttat gaggtacaaa gataaagaaa tcattgttct gacggatctt
    16501 tttggcggaa gtgtcaacaa cgaattctta caatatatca atcgggatca tgtctatctc
    16561 gttgccggat tgaatttgcc actattgatt gagctagtat ctcggttgga cgctgaagta
    16621 gagacaactg ttttgatcca gcaggtgcta actgattcaa aaaaaatgat ccagttttgt
    16681 aacgagcggc tcaatcagaa aatagaagaa gacgaatttt aggaggaaga aaaatgattg
    16741 tattgacaag agtcgaccat cggttattac acggacaagt agctttttca tggacacaaa
    16801 caataggcgc agactgtatt ttgattgcta atgatgatgt acctacgaat gagatacgaa
    16861 agacaacgat caaattagcc aagccacaag gagtcaaact agtgatcaaa tcaattgatg
    16921 actcgattgc cgcgttgaaa agcggggtga cagataaata taagttattc atcgtagtgg
    16981 aatcgatcga ggatgcatat aagctggcag aagcgtatcc agaaatcaaa accatcaact
    17041 taggagggat caaggcaaaa cctggtactc gcagcatcgg aaaagcggta aatattttac
    17101 cagaagagga ggaacgcttg aaacgattaa ttgacaaaca aatagaagta gaaatccgtc
    17161 aagtaccagc agataaaaaa gtggatgtaa gagaagtttt gtaagaaagg aagaacctca
    17221 atgattgtac aagcagtctt attaggaatt gttgcatttg tcgcacaatc agaatatgca
    17281 ctagggacat cgttgctttc tcgtccgatt gtgacggggc tgtttacagg aatcgtgtta
    17341 ggtgatgtaa aaactgggat cattatgggc gcaactttag agttagcatt tatcggatcg
    17401 ttctcagtag gtgcttcgat tccacctgat gttgtgaccg gtgggatttt agggacggct
    17461 tttgctatta cagctggagc cggtacagaa accgcgcttt tactaggttt gccgattgct
    17521 acgttgacat tgatattgaa aaatatttac ttagggcttt tgattccaat tatgaaccat
    17581 aaagcagaca attatgcaga agaaggaaat tataaaggag tcgagcatat gcacttgctt
    17641 gccggtatcg gtctttcctt gatgcttggt gttgtggtaa tggtttctta tatggtggga
    17701 agcaatacga tcggaaatct attaaatatg attcctgact tcgttcaaaa ggggttgtca
    17761 gtagctacag ggttgattcc agctcttggt tttgcgatgc ttgctcgttt gctgatcaac
    17821 aaacaagttg caccatattt tttcttaggt tttgctatag cttcttactt agagattcct
    17881 gtgaccggag tagctatttt tggtgcgatt ttagcagttg tggtagtcaa tattatgaat
    17941 gtccgtcaac taccacttca aacaactgca ggggaggtag aagaagatga agaattctga
    18001 gatcaaaaac agcaaaataa cgaaaaaaga gctaaatcac gtattttggc gttcttttca
    18061 aatggagttt tcttggaatt atgaacgtca gatgaatatg gcttatgttt atgcaatgat
    18121 tccgattttg aaaaagttat atcagtcgaa agaagaaatg gcagcagctt tgaaacggca
    18181 tctagagttt tttaacacga ccccacatat cgttacactg attttaggaa tcacggctgc
    18241 aatggaggaa gaaaataagg atgatcctga atttgatgtg actgcgatcg atagtatcaa
    18301 aacatctttg atgggacctc ttgcagggtt aggagattct ttcttttggg gaactttgcg
    18361 attgattgcc acaggtgtgg gtacctcttt agccatgcaa ggaaatattt taggccctat
    18421 cttgttcatt ttgattttca atattccgca cattctgttt cgctatctag caacaggttg
    18481 gggatatcgc ttaggaacgg gttttttgaa aaagatccag gcaaacggta tgatggaaag
    18541 tttgacatta ggcgcgtcta ttattggact gatggtcgtt ggagcaatga ctgccacgat
    18601 gatcaccatc aatattcctt taaaaatcgg ttcaggagaa aatgctgtga ctgttcaaag
    18661 tatttttgat gatatcgttc caaatatctt atgtcttgga gcatttggca tagtgtttta
    18721 tctattgaag aaagaagtga aaccattaac gatcttgatc ggactagctt tctttggaat
    18781 ttttggttcg tggataggga ttttttaaaa aaggagcaga gaaaatgttt acgatgcaag
    18841 attatatcta tgaagaaaag gaagtactaa gtacgatctt gaaaaagaat gatttttcga
    18901 caagagaaca tatgaaaaaa acagtgaatt tactgatctt agcaacgggt tcatcttata
    18961 acgcttgttt agcagcgaag ccagcattgg aatcttatgg tgatcttact gtcgatattc
    19021 aagaaccttt ttattttttc aattatggaa aactttctcc ttcaatcgat actgtgattg
    19081 ctgtgtcaca aagcgggaaa agtgcttcta ctgttgaagc agtaaaaatg atacaaaaac
    19141 aagggttacc tgttgttgct atcacaaatg acgtgcaaag tccacttgct ttagaagctg
    19201 cacaaattat tgatttaggt gccggggtag aatcagtagg cttcgttaca aaaggctatt
    19261 ctgcgactgt tttacagtta ctactcttag ggataggaat tggaataagt aaaaataaaa
    19321 tcagcaaaaa aatagaacaa gactacatac aacagttgcg caaaataatc aaccatttac
    19381 cagcgattat tcaaaaaacg gaagaatttt ttgatggata tcaaagttta tttcgtttag
    19441 cacagcgttt cgtcaccatt ggatatggtc caaactgggg aacagctaaa gaagcagaga
    19501 cgaaattgac tgaaacaatc cgcgttccgt cacaaggatt tgagttagaa gcttatatgc
    19561 acggcccata cctggaggca gatgcttcgc atttgctatt ctttattgaa ggggaaagtg
    19621 taaataagga acgttcgcaa aagttgcaaa gatatatgag tcggtacgtt ggagagactc
    19681 tcaccattac cacaaaaaaa gcaagaaacg agaaaacact tggattagca atagaatgtg
    19741 atgaatatct gtctgtatta gcgcttgtcg ttccgtttca actattcgct tataaaaccg
    19801 cagtagctaa aggtattgac ttgaacaaaa agatttttga agactttgat acagtcttga
    19861 aaagtaaatt ataagaggtg gaaatcatgc tgaaattcaa tgaagaagaa caaatcaaag
    19921 caataaaagg cgcactagct ttacgtccgc aagtagaaga aatcatcgat aaactttatg
    19981 cggaaaaatt tgacgctgtc tattatctag gaatcggtgg aacgtatgct tcctcgatgc
    20041 aagctgtcac ttatatgaat gggaaaagca acttgcctgt ctttgtacag catgctgcag
    20101 aatattacac tactggaaac aaaagattaa caaaagattc tatcgttatt ttgtcctctg
    20161 ttaccggtac aactcaagaa gtagtgaaag cagtagaaca aatcaagaaa gtaggagcga
    20221 cactgattgg atttatcgat aaagcgaaca gtaagttgtc tcagctatgc gattttgtgg
    20281 tcacttatcc agctcctgga acagaacaaa tcaaattttt tatggcagca gatcgattga
    20341 tgtatttgaa tggagaattc gaagcttatt cagaatacta tgaacagctc gaacgttatt
    20401 taccaacagg gttagtggaa gcagagaaaa aagcagatgc atttggctta tcatttgcag
    20461 aaaaacatcg acgtgatagt atgcattatt ttattggagc agggaatcaa tggggagctg
    20521 tttattcata tgccatgtgt tactgggaag aacaaagctg gcttccatcg aaatctatcc
    20581 atgctgccga gtttcttcac gggacgttag agatcgtaga agaaacaacc ccagtcacat
    20641 tatttttagg cgaagatgaa cagagagtgt tatctgaacg tgtggctaaa cttttgccaa
    20701 aaatttgcag caattataca ttgatagaca caaaagacta tccagtagaa ggtatcagtg
    20761 aaaaatatcg cggacgtgtt ttgtctttct tattgatgca tgtagtgaca caacgcatcg
    20821 atgctcatgt ggagcagttg aactgccatc cattagagat acgccgctac tatcgtcagt
    20881 ttgattatta ggatagactg gataaaaaga atataaaaaa taaagagagc agcgataact
    20941 gctcccttta tttttttaaa atattggcat caattacgtt ataaaacaca ttttttgcat
    21001 tatctacagt tcaacttata atctgtttaa tgggacatgt gggtgccaac cattttttgt
    21061 tatgcaatag tactatttag ttgttgcatg gatccgtagt aaaagtccaa gtacaacgag
    21121 ttgcgccagt ttacatagcg ttttgtatcc agaatgttga ctaaaacaaa agttcctatg
    21181 tcaaccgtca acagagacaa tctgtccgtt gcaggacctg acagatttag aaatgttcaa
    21241 tctgtctctt atgtatttta aatcaatact cactaggaga agtttcctaa cctcctaata
    21301 gtaatgctgg tagtaacatt gtcaaaactg cttcctctta ggaacaagca aagcataacg
    21361 agtgtaaaaa taaaatgtag actattgtaa catatcattg attatttcaa gtaaaaacga
    21421 taaaagaagt aaacaaacac ttaaaaacaa tggaaattag tcccaacttt aagaaatgta
    21481 tactataatg gaaattatca attatttatt tggaggaata tccatgaaac aaaaaatcgc
    21541 agttcttggt cctggatctt ggggcactgc tttagcacaa acattggcag aaaacgggca
    21601 tgatgtccgt atttggggaa atgtacctga acagattgat gaaatcaata agtatcatac
    21661 aaatcagcat tttttacctg atttgacgat cccagaatcg attatcggat ataaagaatt
    21721 ggcagaagca gtggataacg cagatgctat tttatttgtt gttccaacaa aagcgattcg
    21781 ttcagtcgca caggaactta ttggtaaaat gaatactaag ccaatcatta tacatgcaag
    21841 taaaggacta gaacaagata cgcataaacg aatctcagaa gttttggctg aagaaattcc
    21901 agaagaaaaa agacaggcaa ttgttgtatt atctggtcct agccatgctg aggaagtggc
    21961 agtacatgat atcacaacaa ttacagctgc aagtattgat caaaaagcag cagcctatgt
    22021 acaaaaactg ttcatgaatg attatttcag gatttataca aataatgatg tgattggcgt
    22081 agaaactggt gcagcactga aaaatatcat tgcaattggt gccggcgcta tccatggatt
    22141 aggattcgga gatgatgcaa aagcagcaat catgactcga ggattggcag aaatcagccg
    22201 tcttggtgtc gcgatgggtg ccaatcctct gacatttatc ggtttgagtg gtgtggggga
    22261 cttgatcgtt acttgtacaa gtgttcattc tcgcaattgg cgtgccggta acttgttagg
    22321 acagggacat aagctggaag aagttctaga aaacatggga atggtcgttg agggtgtggc
    22381 aacgacaaaa gcagcagtag agctagctca acagctaggt gttgagatgc caattactca
    22441 aacaatctat aatgttttgt ataatggaga agatatcaaa aaagcagcaa aagaaatcat
    22501 gttacgtgac ggaaaaatgg agaatgaatt ttctcttcgt taaatagatg atcaatagtt
    22561 aatcgtcgtt taggaggaac agcaagtgaa ggtcaaaaaa gcagtcattc ctgccgctgg
    22621 gctaggaaca cgttttttgc cggcaacaaa agccatggca aaagaaatgc ttccaatcgt
    22681 ggataaacca acgatccagt ttattgtgga agaagcattg gattcgggaa tcgaagacat
    22741 cttgatcgta acgggaaaag aaaaaagacc cattgaagat cactttgatg caaatatcga
    22801 attggaaaac aatttgcggg aaaaggaaaa aacagaatta ctcgctttag ttgaagaaac
    22861 gactcaagtc aatcttcact tcatccgtca atctcatcca agaggactgg gggatgccgt
    22921 gcttcaagca aaagcattta taggaaatga accgtttgtc gtcatgctgg gggatgattt
    22981 gatgaaagat gatatcccat tgactcaaca attgatgatg gattatgaag aaacacaagc
    23041 ttccgctgtt gccgtcatgc gtgtaccaga aaatgaaaca tctaaatacg gaattattga
    23101 tcctaaaaca gatagcgaaa agggtcgttg cagagtaaaa gggtttgtag aaaaacctga
    23161 aatgggaaaa gcaccaagta atttagccat tatcggacgt tatctactga ctcctaaaat
    23221 atttgagata ttagaaactc aagaaccagg agcaggaaac gagatacagc taacggatgc
    23281 acttcagact cttaaccaga cagaggcggt ctatgcgaga gagtttaaag gaaaaagata
    23341 cgacgtaggc gataagttag gatatatgaa gacaaatatt gaatatggac ttcaacattc
    23401 ggaaattggg acttcattaa gtgcatatat tatccagttg agtaaaaagc taagaacaga
    23461 aagttaactg gaaataagtg gcctgctgag aatgaaaggc tatttgttat taaagcggca
    23521 gcttgtgcgt catttgcgca actgtcgttt tagttttttg actctagatc agttgagtcg
    23581 tggaaaacga aagatagaaa aagactttcg tgaatagatt cagaaaatat tatgctaatg
    23641 gtttaatttt tttttaagtt gtataaaaga aaatgattac ataagagaaa tgaaagccaa
    23701 gccagaagat tcatagtaga ataaaaaggc gaatttttta attataaaaa aaggtgtgtg
    23761 ttcactatgg gaaaattagt cgctgttgca cagtcgctgc ttgatatact caagtggctg
    23821 atggaaggaa gcaattggat atttgtgttg ccactattgc ttattagtct atttatcatg
    23881 tttcgcatgg tcagaaaaaa agaaacgttt ctgattttta tctggtatgc agcatccgtt
    23941 ggggttgcta ccttgatggt tgaattggcg ttaggtagtt gggaagcttg cgtatttgct
    24001 ttattttttg ctgtactgta tctaatcatt tatatcccat atatgaaaag aaagtctcgg
    24061 ataggcagaa aaggtagaca tacaactact ttttctagta cagattctct cgaacaaagt
    24121 agaagaacga gaagaagacg gtaatattta gtaggtatac gcaaaaaaag ataaaataat
    24181 ggataaacta ttgagatgag agatttattt aattggtgga aaaaagcttt tttcacttat
    24241 ataaaagaat cttttttaaa ggaatgcttg ttgtatcggg ataagccatt ccttttttta
    24301 tgttatttat atatatataa atcataccaa t
//
LOCUS       DACBZN010000033        23578 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.033, whole genome shotgun sequence.
ACCESSION   DACBZN010000033 DACBZN010000000
VERSION     DACBZN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.033"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            44..676
                     /locus_tag="IVD64_001312"
     CDS             44..676
                     /locus_tag="IVD64_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6148572.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(678..1583)
                     /locus_tag="IVD64_001313"
     CDS             complement(678..1583)
                     /locus_tag="IVD64_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6148573.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1668..2321)
                     /locus_tag="IVD64_001314"
     CDS             complement(1668..2321)
                     /locus_tag="IVD64_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6148574.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            2643..3587
                     /gene="mvk"
                     /locus_tag="IVD64_001315"
     CDS             2643..3587
                     /gene="mvk"
                     /locus_tag="IVD64_001315"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP6148575.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3580..4557
                     /gene="mvaD"
                     /locus_tag="IVD64_001316"
     CDS             3580..4557
                     /gene="mvaD"
                     /locus_tag="IVD64_001316"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP6148576.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4570..5655
                     /locus_tag="IVD64_001317"
     CDS             4570..5655
                     /locus_tag="IVD64_001317"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP6148577.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5652..6716
                     /locus_tag="IVD64_001318"
     CDS             5652..6716
                     /locus_tag="IVD64_001318"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP6148578.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6965..7768)
                     /locus_tag="IVD64_001319"
     CDS             complement(6965..7768)
                     /locus_tag="IVD64_001319"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP6148579.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7788..8630)
                     /gene="kduI"
                     /locus_tag="IVD64_001320"
     CDS             complement(7788..8630)
                     /gene="kduI"
                     /locus_tag="IVD64_001320"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP6148580.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(8646..9653)
                     /locus_tag="IVD64_001321"
     CDS             complement(8646..9653)
                     /locus_tag="IVD64_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP6148581.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(9660..10304)
                     /locus_tag="IVD64_001322"
     CDS             complement(9660..10304)
                     /locus_tag="IVD64_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6148582.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(10404..11162)
                     /locus_tag="IVD64_001323"
     CDS             complement(10404..11162)
                     /locus_tag="IVD64_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP6148583.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(11289..12440)
                     /gene="fucO"
                     /locus_tag="IVD64_001324"
     CDS             complement(11289..12440)
                     /gene="fucO"
                     /locus_tag="IVD64_001324"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP6148584.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(12522..12833)
                     /gene="rhaM"
                     /locus_tag="IVD64_001325"
     CDS             complement(12522..12833)
                     /gene="rhaM"
                     /locus_tag="IVD64_001325"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP6148585.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(12835..13680)
                     /gene="rhaD"
                     /locus_tag="IVD64_001326"
     CDS             complement(12835..13680)
                     /gene="rhaD"
                     /locus_tag="IVD64_001326"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP6148586.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(13694..14950)
                     /gene="rhaA"
                     /locus_tag="IVD64_001327"
     CDS             complement(13694..14950)
                     /gene="rhaA"
                     /locus_tag="IVD64_001327"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP6148587.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(14947..16407)
                     /gene="rhaB"
                     /locus_tag="IVD64_001328"
     CDS             complement(14947..16407)
                     /gene="rhaB"
                     /locus_tag="IVD64_001328"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP6148588.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            16560..17429
                     /locus_tag="IVD64_001329"
     CDS             16560..17429
                     /locus_tag="IVD64_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6148589.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(17555..18454)
                     /locus_tag="IVD64_001330"
     CDS             complement(17555..18454)
                     /locus_tag="IVD64_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6148590.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(18619..19443)
                     /locus_tag="IVD64_001331"
     CDS             complement(18619..19443)
                     /locus_tag="IVD64_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6148591.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(19454..20749)
                     /locus_tag="IVD64_001332"
     CDS             complement(19454..20749)
                     /locus_tag="IVD64_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP6148592.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(20765..21244)
                     /locus_tag="IVD64_001333"
     CDS             complement(20765..21244)
                     /locus_tag="IVD64_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP6148593.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(21250..22278)
                     /locus_tag="IVD64_001334"
     CDS             complement(21250..22278)
                     /locus_tag="IVD64_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP6148594.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(22295..23401)
                     /locus_tag="IVD64_001335"
     CDS             complement(22295..23401)
                     /locus_tag="IVD64_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6148595.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
BASE COUNT         6779 a         5200 c         4239 g         7360 t
ORIGIN      
        1 tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa aaatcgaacc
       61 tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag atgaggagat
      121 cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca aaaatatggt
      181 attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac gtcattttga
      241 atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga ttggtggcag
      301 tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg ccgaaagtta
      361 tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta gtagtacaaa
      421 cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt atgaggtcac
      481 ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt ttcgaacttc
      541 tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat ctcaagattc
      601 tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag cccttggttt
      661 gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat ccaaatagat
      721 aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt aaagggtaac
      781 tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat cagcgaccag
      841 accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt ataagtgcca
      901 gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg tcgtgcttcg
      961 atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata aatagccaat
     1021 gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa gtaaaccaaa
     1081 ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc tcttaatttc
     1141 agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa ggaatcagaa
     1201 atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat tcgttccaaa
     1261 agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt aaacaaaccg
     1321 tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac tttttgctgg
     1381 tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc tacgcgcgcc
     1441 cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata ttcttctttt
     1501 aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt acggtcttga
     1561 ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca gtataaagaa
     1621 aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca aatggctact
     1681 ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc ttcttttgct
     1741 tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg actttctttg
     1801 tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc gtatagactt
     1861 ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc caacatcgta
     1921 tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc agcataggga
     1981 agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt ttctgttgct
     2041 cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga acggtccaat
     2101 acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt caaaaaataa
     2161 atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa gacttcatta
     2221 tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat ttctgttaaa
     2281 cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta cctccagctc
     2341 acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc caatatctag
     2401 tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa
     2461 aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg gttttagtaa
     2521 aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg aagcagcgca
     2581 actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga aggagttaga
     2641 ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg ggcgagcacg
     2701 cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat
     2761 tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat tcaggaaatt
     2821 tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac
     2881 agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct gctgaacgtg
     2941 gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat gattacttag
     3001 catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa aaaatcgccc
     3061 acggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg atttatttta
     3121 caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg attgtcgctg
     3181 atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa
     3241 cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg aagcaagcaa
     3301 aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat gaatcacaat
     3361 cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc caaacagcaa
     3421 aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc tgtatgattg
     3481 cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa gatgcgggtg
     3541 ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg caaagcacgc
     3601 gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga actcatcctt
     3661 ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac agaagtcatc
     3721 ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca agacgaaaaa
     3781 gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg cctctcacta
     3841 aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc ctcctctgcc
     3901 agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg attagatgat
     3961 ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag tattttcggt
     4021 ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa gccagtccct
     4081 tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa
     4141 gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt ttatcaaggc
     4201 tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa aacaaaagat
     4261 ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg aacgacttta
     4321 gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat ggatgcggtg
     4381 cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg ccctaatgtc
     4441 aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac caaattattt
     4501 agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat tgaataaagg
     4561 gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg agaatatgca
     4621 gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt gacagtcact
     4681 gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg gatgcctgta
     4741 cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc ttttcattat
     4801 atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat tcttttatcc
     4861 ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa atatggattg
     4921 ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg
     4981 aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc agttcaagat
     5041 aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc tttctcaacc
     5101 ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga gttacttgcc
     5161 ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt acgtttattg
     5221 attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt tcaccgttcg
     5281 agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga atgtgtcaac
     5341 gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat gattcgaaaa
     5401 aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga aacgcctgct
     5461 ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc ttctggtgca
     5521 ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct tcctttaatg
     5581 agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag cgatcaaaga
     5641 aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc tttcatgata
     5701 aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg caaatagcag
     5761 tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc ccattttata
     5821 tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg
     5881 tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc ttgaaagtcc
     5941 cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg aaaatcattg
     6001 ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga ttgtttcacg
     6061 cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct gagggagacc
     6121 gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt gacgtccccc
     6181 tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat ctagctgctt
     6241 taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc cagatcgaaa
     6301 acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa tcgacagtct
     6361 cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc tccggaggta
     6421 tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc gtgggtactt
     6481 caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt atactgatga
     6541 agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg aataccgcgg
     6601 ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc gaagcaagag
     6661 ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa
     6721 cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa ctccttcgga
     6781 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt cttatttctt
     6841 ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc
     6901 cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc
     6961 tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc
     7021 cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg
     7081 gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt
     7141 caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc
     7201 caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca
     7261 tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca
     7321 tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg
     7381 cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg
     7441 catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa
     7501 ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca
     7561 catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat
     7621 caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc
     7681 gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact tttccatcta
     7741 aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta ttttaattga
     7801 tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg
     7861 aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct
     7921 tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca
     7981 cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta
     8041 tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat
     8101 tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc
     8161 aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac atttggatat
     8221 ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa
     8281 gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct
     8341 tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca
     8401 cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt
     8461 tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt
     8521 ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa
     8581 tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac
     8641 tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga
     8701 ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa
     8761 tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa
     8821 gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca
     8881 tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta
     8941 cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa
     9001 taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca
     9061 tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg
     9121 gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca
     9181 gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct
     9241 ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat
     9301 tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg
     9361 cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc agataatacc
     9421 tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta
     9481 tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg
     9541 tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc
     9601 tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc
     9661 tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca
     9721 aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca
     9781 gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact
     9841 tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat
     9901 agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat
     9961 cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa
    10021 gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat
    10081 actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg
    10141 attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg
    10201 atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc
    10261 actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc
    10321 ctatttagaa attttatttt ataacttgga aatctatacc catattatac ggcagaaaag
    10381 aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt
    10441 tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata
    10501 cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa
    10561 atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgtttggatt
    10621 tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc
    10681 ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc
    10741 gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt
    10801 tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata
    10861 gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg
    10921 atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc
    10981 tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta
    11041 gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct
    11101 aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc
    11161 atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa
    11221 agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg
    11281 cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt
    11341 ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct
    11401 tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg
    11461 atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc
    11521 cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg
    11581 acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac
    11641 gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata
    11701 tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga agcttccaaa
    11761 gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct
    11821 tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat
    11881 tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga
    11941 ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt
    12001 acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc
    12061 gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc
    12121 ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc
    12181 tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg
    12241 atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt
    12301 gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc
    12361 tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt
    12421 aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa
    12481 acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc
    12541 atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac
    12601 ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg
    12661 atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg
    12721 accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg
    12781 tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac
    12841 acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc
    12901 tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac
    12961 gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat
    13021 gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat
    13081 tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga
    13141 aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa
    13201 ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg
    13261 cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact
    13321 tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt
    13381 gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt
    13441 aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa
    13501 aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc
    13561 ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt
    13621 cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa
    13681 tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt
    13741 ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg
    13801 catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca
    13861 gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg
    13921 tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca
    13981 gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca
    14041 attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca
    14101 acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg
    14161 gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat
    14221 aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta
    14281 ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca
    14341 ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc
    14401 aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc
    14461 ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa
    14521 agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt
    14581 ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga
    14641 cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca
    14701 acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa
    14761 aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga
    14821 catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag
    14881 catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa
    14941 tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt
    15001 attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg
    15061 ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt
    15121 ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag
    15181 tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa
    15241 gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg
    15301 agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat
    15361 ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg
    15421 cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg
    15481 cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt
    15541 gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc
    15601 gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc
    15661 tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa
    15721 ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt
    15781 tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc
    15841 tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc
    15901 cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg
    15961 tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg
    16021 gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc
    16081 tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg
    16141 ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat
    16201 ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt
    16261 ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta cttcttccaa
    16321 gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat
    16381 atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc
    16441 actttattaa taaagcgtta tcatttatta acaatgctat cataatacag aatcttatat
    16501 tttgcttcac actattttgg tatataattg cattatcttg acaagttggg agtgtttaca
    16561 tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga
    16621 cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag
    16681 caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt
    16741 tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact
    16801 tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg
    16861 aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa
    16921 aagacggcca taacaccttc cattcaacag gggatccatc agaaaacaag atccaccatt
    16981 tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct
    17041 ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg
    17101 aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca
    17161 aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa tatcagctga
    17221 gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac
    17281 tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag
    17341 aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat
    17401 cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa
    17461 tattttgaaa tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac
    17521 agcagctact gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat
    17581 aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact
    17641 ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag
    17701 cactagagct gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta
    17761 catactgatg aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc
    17821 tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga
    17881 tatattgtaa catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac
    17941 tctcttcttt ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat
    18001 attttatgtc ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt
    18061 gcttcaatac ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa
    18121 agcaagccga tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa
    18181 agatatagac acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg
    18241 aatggatgag cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag
    18301 tcgcttctcc ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt
    18361 tattttggct gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat
    18421 tgacttgttc accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta
    18481 tgcacaattt tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt
    18541 ataaaaaaga agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta
    18601 accaacgtct tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt
    18661 tgtttcttca agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt
    18721 tgtgtactcg atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg
    18781 aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac
    18841 caattggact tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat
    18901 tcctagatag tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac
    18961 cggatgattc aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac
    19021 caagcgacgg ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt
    19081 atattggatt tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga
    19141 agaagcatag cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc
    19201 tggatgaatc atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa
    19261 aactcgtttg acataatttc ccatcccaga attgatctca tttgcgccag aaggtatttc
    19321 aggaaacgaa agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc
    19381 tgcaagatct tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa
    19441 cataactatt ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag
    19501 taatcaaaaa caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg
    19561 cctctgcttt tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg
    19621 tgatggagcc tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc
    19681 ctacacttgt gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat
    19741 ccataaacat acccaccagt aataacacaa gattgactaa taacagaata ataaatttat
    19801 tatccgttac gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg
    19861 cgaaagacat catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg
    19921 attgaaacaa catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa
    19981 tcaacaggga gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca
    20041 agataccacc gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt
    20101 ttccagcatg agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga
    20161 tgaatgctac tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa
    20221 acaaagcaga gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg
    20281 gaggaatgac catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt
    20341 catatccttc ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa
    20401 tggccgagct agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag
    20461 cccccggcac gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc
    20521 cattattcat gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat
    20581 tgatacttcc caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta
    20641 acaataaagt catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa
    20701 gcaacacaac aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc
    20761 atttttatat tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca
    20821 ggctatagat aatgatgagt acccccgata caggtactga cagataaaca tatcccatag
    20881 gaatacttaa agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat
    20941 acaccatgat catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt
    21001 tacttttttt gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc
    21061 ccacaacata cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt
    21121 ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag
    21181 cgaccatccc aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt
    21241 tcatcacagc tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc
    21301 gtttttgaat atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc
    21361 attgaattgg actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc
    21421 aaaagcttca atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg
    21481 ctcatcagtt agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt
    21541 atgctcatca taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa
    21601 ttcattgttt tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact
    21661 tcccataggt acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg
    21721 catcacacgg atcttctttc ccttcaaatc ttccggcgta tggatcggac cgtctaccat
    21781 ataaaagctg cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt
    21841 cgattggtag attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc
    21901 atcattgaat agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc
    21961 tgaggcactt acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg
    22021 ttctccaccc agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc
    22081 ttttactaaa cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata
    22141 agcaaagcgc aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga
    22201 attatttccg cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag
    22261 cagtgatgtt tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg
    22321 tcaaacaaag caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat
    22381 aaaaatctaa actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt
    22441 catcgatttg ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg
    22501 gtaaataacg tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag
    22561 attctacgta agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat
    22621 aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat
    22681 cttctttcga taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt
    22741 ttccacgagc ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat
    22801 accacaatcc tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag
    22861 cttcttctaa atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg
    22921 tcatcatcaa tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct
    22981 gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta
    23041 aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg
    23101 gtgccattgt attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt
    23161 cattgaccac ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat
    23221 atagaccgac accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc
    23281 catcaaagtc caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga
    23341 gcaggtcaat tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca
    23401 tctttacgcc acttttttaa acgctttcat tttatatcct taatatagca gtaataccgt
    23461 ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa
    23521 caaaagtaac tttcagttac ttggaaatta aaaaaagaac ttggagaaac aacgtttt
//
LOCUS       DACBZN010000034        23571 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.034, whole genome shotgun sequence.
ACCESSION   DACBZN010000034 DACBZN010000000
VERSION     DACBZN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.034"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVD64_001336"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVD64_001336"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP6148596.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IVD64_001337"
     CDS             complement(1172..1867)
                     /locus_tag="IVD64_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP6148597.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IVD64_001338"
     CDS             complement(1880..2596)
                     /locus_tag="IVD64_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP6148598.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IVD64_001339"
     CDS             2926..3531
                     /locus_tag="IVD64_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP6148599.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IVD64_001340"
     CDS             3856..4179
                     /locus_tag="IVD64_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6148600.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IVD64_001341"
     CDS             4361..5731
                     /locus_tag="IVD64_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP6148601.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IVD64_001342"
     CDS             complement(5861..7162)
                     /locus_tag="IVD64_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6148602.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IVD64_001343"
     CDS             complement(7392..9383)
                     /locus_tag="IVD64_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP6148603.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IVD64_001344"
     CDS             complement(9527..9793)
                     /locus_tag="IVD64_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6148604.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IVD64_001345"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IVD64_001345"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP6148605.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IVD64_001346"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IVD64_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP6148606.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="IVD64_001347"
     CDS             13137..13700
                     /locus_tag="IVD64_001347"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6148607.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="IVD64_001348"
     CDS             complement(13953..14474)
                     /locus_tag="IVD64_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP6148608.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="IVD64_001349"
     CDS             complement(14477..15319)
                     /locus_tag="IVD64_001349"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP6148609.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="IVD64_001350"
     CDS             complement(15312..16037)
                     /locus_tag="IVD64_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP6148610.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="IVD64_001351"
     CDS             16213..16509
                     /locus_tag="IVD64_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148611.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="IVD64_001352"
     CDS             17077..18669
                     /locus_tag="IVD64_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6148612.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="IVD64_001353"
     CDS             18682..19422
                     /locus_tag="IVD64_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6148613.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="IVD64_001354"
     CDS             19590..20939
                     /locus_tag="IVD64_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6148614.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="IVD64_001355"
     CDS             complement(21067..21657)
                     /locus_tag="IVD64_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6148615.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="IVD64_001356"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="IVD64_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6148616.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6749 a         4747 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct ccgatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DACBZN010000035        23141 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.035, whole genome shotgun sequence.
ACCESSION   DACBZN010000035 DACBZN010000000
VERSION     DACBZN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.035"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            280..804
                     /gene="infC"
                     /locus_tag="IVD64_001357"
     CDS             280..804
                     /gene="infC"
                     /locus_tag="IVD64_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP6148617.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            876..1076
                     /gene="rpmI"
                     /locus_tag="IVD64_001358"
     CDS             876..1076
                     /gene="rpmI"
                     /locus_tag="IVD64_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP6148618.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1129..1488
                     /gene="rplT"
                     /locus_tag="IVD64_001359"
     CDS             1129..1488
                     /gene="rplT"
                     /locus_tag="IVD64_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP6148619.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1740..3047
                     /locus_tag="IVD64_001360"
     CDS             1740..3047
                     /locus_tag="IVD64_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP6148620.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3124..5253
                     /locus_tag="IVD64_001361"
     CDS             3124..5253
                     /locus_tag="IVD64_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP6148621.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5228..5917
                     /locus_tag="IVD64_001362"
     CDS             5228..5917
                     /locus_tag="IVD64_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148622.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(6325..7011)
                     /locus_tag="IVD64_001363"
     CDS             complement(6325..7011)
                     /locus_tag="IVD64_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6148623.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(7147..7341)
                     /locus_tag="IVD64_001364"
     CDS             complement(7147..7341)
                     /locus_tag="IVD64_001364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148624.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(7391..7831)
                     /locus_tag="IVD64_001365"
     CDS             complement(7391..7831)
                     /locus_tag="IVD64_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP6148625.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(7835..8680)
                     /locus_tag="IVD64_001366"
     CDS             complement(7835..8680)
                     /locus_tag="IVD64_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP6148626.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            8829..10247
                     /gene="pepV"
                     /locus_tag="IVD64_001367"
     CDS             8829..10247
                     /gene="pepV"
                     /locus_tag="IVD64_001367"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP6148627.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(10304..11251)
                     /locus_tag="IVD64_001368"
     CDS             complement(10304..11251)
                     /locus_tag="IVD64_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6148628.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11474..11824)
                     /locus_tag="IVD64_001369"
     CDS             complement(11474..11824)
                     /locus_tag="IVD64_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6148629.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12024..13103
                     /gene="pepA"
                     /locus_tag="IVD64_001370"
     CDS             12024..13103
                     /gene="pepA"
                     /locus_tag="IVD64_001370"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP6148630.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13247..13567
                     /locus_tag="IVD64_001371"
     CDS             13247..13567
                     /locus_tag="IVD64_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6148631.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            13589..14053
                     /locus_tag="IVD64_001372"
     CDS             13589..14053
                     /locus_tag="IVD64_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6148632.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            14258..14863
                     /locus_tag="IVD64_001373"
     CDS             14258..14863
                     /locus_tag="IVD64_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP6148633.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(14902..16191)
                     /locus_tag="IVD64_001374"
     CDS             complement(14902..16191)
                     /locus_tag="IVD64_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6148634.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            16619..17098
                     /gene="rlmH"
                     /locus_tag="IVD64_001375"
     CDS             16619..17098
                     /gene="rlmH"
                     /locus_tag="IVD64_001375"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP6148635.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            17183..17692
                     /locus_tag="IVD64_001376"
     CDS             17183..17692
                     /locus_tag="IVD64_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP6148636.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            17792..18442
                     /locus_tag="IVD64_001377"
     CDS             17792..18442
                     /locus_tag="IVD64_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148637.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18893..19831
                     /locus_tag="IVD64_001378"
     CDS             18893..19831
                     /locus_tag="IVD64_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6148638.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19844..20894
                     /locus_tag="IVD64_001379"
                     /pseudo
     CDS             19844..20894
                     /locus_tag="IVD64_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            21160..21795
                     /locus_tag="IVD64_001380"
     CDS             21160..21795
                     /locus_tag="IVD64_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6148639.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            21986..22777
                     /gene="msrA"
                     /locus_tag="IVD64_001381"
     CDS             21986..22777
                     /gene="msrA"
                     /locus_tag="IVD64_001381"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6148640.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7376 a         4068 c         4788 g         6909 t
ORIGIN      
        1 ggaatcactg ctagaaagtc tgctaatcta atcattttca gaaaaggaag acaaactgta
       61 tgaaaaacat tgacaaaaag attagttttt gctatactat gatggttgag aaataaaagc
      121 agaagcaccc gcttctcgcc ttagttgata atgtcactgg gctagatagt tttcactggt
      181 tctattaggt gaagtacgcg cgggttctgt aagagaactc gtttttttat tgccgtttat
      241 tcggtaagct tttctttctc tcataatatt tggaggtgaa tgaccatagc aaaggatatg
      301 atggttaacg acggcattcg tgcacgagag ttacgattga tcggttcaga tggtgagcaa
      361 ttaggagtta agacaaaagc agaagcattg aatattgcag aacaagcaaa cttggatctt
      421 gtgttagttg cccctggtgc gaaaccacca gttgcgcgaa tcatggacta cggtaaatac
      481 cgtttcgaac aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta
      541 aaagaagttc gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa acttcgtaat
      601 gcacgtaagt tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt caaaggccgt
      661 gccattaccc ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg
      721 gatattgcaa cagtggaaca aaaagcgaaa atggacggac gcagcatgtt cttgacgctg
      781 gcaccgaaaa acgacagtaa gtaatttgaa tgactaactg gctgttggac agccgatagt
      841 cggataacga aagattttga ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg
      901 gatcagcaaa acgtttcaaa cgtactggta aaggcggatt gaaacgcttc cgcgcgttta
      961 caagtcaccg tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca
     1021 tggtttctag tggcgacttc aaacgtattc gccaacaact agcaaaaatg aaataagaag
     1081 tacaaataac agaatctgaa gatttatttt tattaggagg aattatacat ggcacgtgtt
     1141 aaaggtggaa cagtaactcg taaacgtcgt aaaagagtgc taaaattagc aaaaggttac
     1201 tacggttcaa aacacacttt atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac
     1261 tacgcatatc gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt
     1321 atcaacgcag cagctcgtat gaatggcttg agctactcaa aattaatgca cggtttgaaa
     1381 gttgctgaaa tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa cgatgcagca
     1441 gcatttactg cattggctga ccaagcaaaa gaagctttgg ctaaataagc taaacaatta
     1501 taaagaggaa agaagataac gaaaagttat gttctttcct ctttttttat ttacaatcct
     1561 ccatcaaatg aaacaggaca ataacgtata agtctaaaat agcataatta aggtttttta
     1621 attgaaaact gaacatttat ggtcaccctt taatgaaagc gctaactaca taagttagaa
     1681 tgagtttgac tgatatatta gtttgcttat cagaaaaaca aaatggacgg aggaataaca
     1741 tgagtgatat tattattggt attttgttag ttatctcatt ctttttcatg gtctggtact
     1801 gtgtaaaagg ctacaacttg atggtcggct ttgccatcat ggctactgta tggatgggat
     1861 tagcgctagt cggaaacacg ttttcaccaa acccggcgat ggaagggcaa ggagtaattg
     1921 atgtgttgac gcatatctat acgacggggc cagctgagta cgccaaatcg atcttggtaa
     1981 atgtcttctt tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc
     2041 tgattcgaaa ggtagttgaa cttggagggg acaagccaag aatcacgatg agcttactat
     2101 gtattgtaac cgctgttata ttcatgtcga tgacaggaat cggaccagtg atttctattg
     2161 cagttattgt gttgccaatc ttgatgtcat taggtatttc agtaccggtt gcactgttct
     2221 catttatggg ctcgattatg gctggaatct ttgctaatat cgtcaatttc aaacaatatc
     2281 aaacgattta tgctggtttc aatccagcgg ctgaaagcta tacttataat gactatttcc
     2341 aaattggtat gattgggatg attgtaagct tagttgtggt actaacggtc gctaatatat
     2401 caatgaacaa aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg
     2461 caccaatgat ctcatggtta gccgtattat taccagtttt aggggtcgtt cttttagatc
     2521 ttccaatcat cttaggtttt attctagcag gtatctgggc attgctgttt actggaaaat
     2581 tgcgcggagg ttataaagaa atctgtcgtc agtttgcgaa attatttaca gatggcgcag
     2641 tagatgttgc accaatggtt ggtttcttga tgaccttagc gatgttcaat aattcagctg
     2701 cgtatgcttc tccttatttt tcagctattt ttggagattg gataccgcaa tcaccattag
     2761 tcttggcaat cgtatttgct atattgacac cactaggatt tttccgcggt ccaatgaatc
     2821 tagtcggttc aggctcagct attttagctg tcgtattagc tgttaatcca acgatgtcgc
     2881 cagccttttt gttcccgtta tttgcaatca caacgattgc tcctcagcat ttagatatta
     2941 cacaatcttg ggttgcttgg ggattaggtt atacaaaagt aacttctcga gaatatatga
     3001 agaaatccat cccaactggt tggatcatcg gagcgatctt tgcctgatca cgttcttatt
     3061 gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag
     3121 aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg gtacacatac gaaagcagtc
     3181 gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat
     3241 gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc aaaattgttt gcgggaaaat
     3301 aatatcagtc cagaagatgt tgtgtttgtt gcacattcta ccacacaagc gactaatgca
     3361 ctgattgaag gagacgtagc aaaagtcggc gtaatcggga tggcaaaagg tggattagaa
     3421 ggttttttag caaaacaaca aactagattg aacgatatcg atttagggaa taagaaaaaa
     3481 atagagattg tcaatgcatt cttacctgtc aaacacttaa atgtagaccg tgtttcagaa
     3541 acgatttctt ctttagaaag agaaagagcg gaggtcttgg tttcttctat ggcatttgga
     3601 gtagataatg gggaaccaga acgagttgtt tatgaagcag catcggtaaa atcgatccct
     3661 acaacgatgg cttctgacat cacaaaatta tatggattga caagaagaac tcggacagca
     3721 gcaatcaatg catctatttt accaaaaatg ctagatacgg caacctctac agaagattcc
     3781 gtacgagaag caggggtcaa tgtttctttg atgattatgc gcggagacgg cggagtaatg
     3841 gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc tttccggtcc agcagcatca
     3901 gtcatgggct ctttgatgta cttgagagcg tctaatggtg tttattttga agtaggcgga
     3961 acaacaacca atatcggggt cattaaaaat ggacgtccag caatcgatta ttcaattgta
     4021 ggtggccacc caacatatat ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt
     4081 tcgatggtac gggctaatca gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt
     4141 gccggtctag attatgcagt atttacggag acagaaaaaa tcaaaggtcc taaggttgaa
     4201 tttttctctc ctaaagaagg agaccctgct gattacgtca aggtagtgat ggaagacgga
     4261 gaagaagtga ctattacaaa tacatgtgca gccaatgtcc ttggattagt gcaagaagaa
     4321 catttttctt atggaaatgt cccatctgca cgaaaagcca tccaagcatt agctgattat
     4381 tgtcatacga cggttgaaga catcgcagaa cagatcatgg aaaaatccta tgctaagatt
     4441 gaaccggtga ttttagaatt ggcagacaag tatcacttgg agaaagatca gatttctctt
     4501 gtaggtgtag ggggcggcgc tgcctcattg attacttact ttagtaataa aatgggtgtg
     4561 aaatattcga ttcctgaaaa cgccgaagtg atttcttcta ttggtgtagc acttgcaatg
     4621 gtccgagatg ttgtagaacg tatcatccct tcgccaagta aagaagatat acgatcgttg
     4681 aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta caccagaatc gatcgaagtc
     4741 cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta tcgctactgg ttctacggaa
     4801 gtgaaagcaa ccgatctaac caaggagatc acaacagaag aagcactaga gcttgctgca
     4861 gaggatatgc gattaaataa aaatgaagtt tgcttattgg aaaatacacc tttcttttat
     4921 gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa
     4981 ggatttatca aggtccaacg tggacatgct tcatgtatga aaacaactgc agccaattat
     5041 atgacagctg tcgaacaact ttgggaagat atggcagttt atcaaactga attgattgcc
     5101 cgaccagaat tttatctctg cttaggagca agagtctctg attttacagc gacggatctg
     5161 gaacaactac agcttttgat ggatctggaa gtatctacta tggaaccgga ggaagaagtg
     5221 atcgtcgttg ccggaaatat caaacaaaca taagttagaa gagttactaa acgaattaca
     5281 acagatcccc gaagaaattt ggggattcta ccagtttcgg cgagatttat tttggaaaaa
     5341 gattcctttg tccaagcaga aaattcttat acaacaatca atcgattgtg gtatcgaaac
     5401 ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta ggagaaattt gtgaacaaat
     5461 ggctgttcca atcgtttcct gtgaatcaga gcaaataaac gaacgaatca cttttgcaac
     5521 ttatgcagaa gatgaaggga ttcgattgat gacggagccg ttagaaaaat taaaatgttc
     5581 aggactagct tcgatctcta aagaaacagc gcaagctttg attattggac atgaactatt
     5641 tcaccacatc gaagcttctg ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg
     5701 gcggttgcct ttttatacac atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat
     5761 gagtttttca aaagagatga atcaaagcga tttttcgcct tatgtattag aggcagtctt
     5821 actttggcca tataatgaaa ttcgtagcca agggatctta gaagaaataa aaaaaataga
     5881 aaaaagatgt acggagtatg atttagatta caaataaaga gactgtgaca aaagtcgtgt
     5941 cacagtcctt tttttccgaa taaacgatgt tctcctgtca actcctcggt cacaagtcaa
     6001 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagatgaa
     6061 cggcaggctc acgacctctt ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt
     6121 ccagccaagt taaaaacata agaagctatt tttacttggc tgttcttatt acttgatgca
     6181 gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat cagctgaccc tcggtattag
     6241 ctcaaaaaaa acggcaaaat atgaggaact attttgccgt ttctttctct aatactcagg
     6301 tcaaaacgct gattccaaaa cctcttattt gatgggtctg tcctgaatat cttcaaaata
     6361 atcggggtaa ttatgaatca cgtattcttg ctcctcgata atcatatgta aatgcatcgt
     6421 gcaaagaaat ccgacttcat caaagttatg atcaatcatt gattgaagca ttgtctgatg
     6481 ttcatctaat actcgacccc agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg
     6541 gttcaagtgc gtactataac tttctagcca atcccaaatt tctcgttttc cagcaatttc
     6601 ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa gcttttttat tttgagcgat
     6661 tactgcctga tcggtttgtt ctagtatttt ttctagttta gcttgatctt gcttggtcat
     6721 tttcgcactg cattcttgca ggatcggttt ttccaacttt tcccgcgtat aacgggctaa
     6781 tgaagcagat cggatatcga tttttgaaac ataagtgccg gactgtggaa tggtttttac
     6841 aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc ggtgtacgcc cgatagctag
     6901 ttgttcttct aacactttat cggagatttt acttccaggt gctaactcgg aataaatgat
     6961 ttgtttacgt atagattcat aagcttgctg ttgcaaatta ttgataacca tttaaccttc
     7021 ctttctatgc agatataatc tattataaca taaatcgctt acaaatcgtg tgggcataaa
     7081 aacacagtct gcaaaaaaag ccctgatagg catgcctatt agagcctcga aaagaaaaat
     7141 attgaatcaa tctggtcgta cctcttgtat cggtttgaca cgaccgccga ttcttttagc
     7201 aaaagcttcg gattcttttt tgtctttgaa aatgagcaga ttgtctaagt ttttttcccg
     7261 agtgcgacag ccagttttat cgatgtaacc gtgcattact ttaacgacat acatggggat
     7321 cacctgcttt tttcttttca ttatacgaac tactcgacac aatacaagaa aaatgcttag
     7381 acggtcttta ttaagaattt acatcatcca aagaagcgta cgcttggaat aactgatcta
     7441 actccggatt gactttgctc agcttcacta atcgttgcga atcagaaagt aaaaaacagt
     7501 gtttactact gccagtagtt tttaattttt tgtcttcagt tcccacaatt tcgtagctaa
     7561 aatccaaccg tgtccccgta tacttttgga tagttggatg gatgctcact ttttctccgt
     7621 aatggatcat ttctttatag ctgcaattta cttccagaac ggggatgatg atcccagatt
     7681 cttcgatttt tttataggga aaccctagat gtaccaataa ggctacccga gcctcttcaa
     7741 accagcggat ataattgctg tgatgaataa tccccatttg atctgtttca taataaaaag
     7801 gttctcgcac ataacctgta aatttctcca tgactcatgc ctcgttttcc attgattttt
     7861 tacaaaaata attcatccat ttcggaagtg cttcgctgat ttgtgtcggc aaaacaatat
     7921 attgttcttt tgccagctgg tctccaatga aactatgaag ataaatagct gctgcaatcg
     7981 tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat catcccagct aaagtgtcac
     8041 ccatgccgcc cgttgccata gcagcagaac ctaaaggatt ggtgtagaag tgctggtgac
     8101 cataaatttt tgtctcatga cttttcaaaa cgagcacagc accgatttcc tgttgcttct
     8161 ttttgttttc tttatcccgt tgttcttgta tcggtagaga actcaaccgt tgccactcca
     8221 tttgatgagg tgtaagtaca gtttgttcgg gatagggtag caaaagatga ttagcagcaa
     8281 gtaaacttaa agcagagcca tcaatcacca gccattgttc ttttgattgg ttttctagta
     8341 cttgtttgaa acgttccaaa ctcttagaag aagtacccaa tccgggacca atcaagagta
     8401 catctgcttg ctttattacg gaaagaaact ggtcagtatc cgtccaaact acaatcattg
     8461 cttctggcag atgactgtga agagcactcc gatttttttc atcagttact acagtcgtga
     8521 gacccgctcc gctgtgtacg gcagctaaag cactcataat gattgctccg ccatattgtt
     8581 cattgccgcc aatcaaaacg atacgtccaa atgttccttt atgtgaaatt tttggacgag
     8641 gtgtgatgat tcttttcact agttgttcgt ttatcagcat ctaaaaacct ccttttactc
     8701 attatagcgt atattttttt tagagaaaaa cagataagaa aaggaattgt cagatttttt
     8761 gaactatttt tttctgtaat ttgtggtatc atgaacacga agacttaaat aagtggagga
     8821 ttaactttat gacaattgat tggcaaaaag aagtagaagc acgtaaagaa gacttgcttg
     8881 aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga tgactcaaaa gtaacaccag
     8941 atgcaccatt tggacctgga cctcgcgacg cattgaaaca tatgttagct tatggcgaac
     9001 gtgatggatt taaagtaaaa aatgtcgata actatgcagg gcatattgat cttggcgaag
     9061 gagacgaaac gctagggata tttggacaca tggatgtcgt tcctgcagga gatggctggg
     9121 acacagatcc ttatgaacca gtaatcaagg atggaaagat ctttgcacgc ggatcaagcg
     9181 atgacaaagg tccaagtatg gcagcttact atgcgatgaa gatcattaaa gaacttgact
     9241 tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga tgaagaaagc ggctggggcg
     9301 atatggctta ttatttcgaa catgaagaag aaccagattt tggtttctca ccagatgctg
     9361 aattcccaat catcaatggt gaaaaaggaa atgtttcttt agctttacgc ttcaaaggcg
     9421 acaatgcagg agattatgtc ttgaaatctt ttgtttccgg acttcgcgaa aatatggtgc
     9481 caggaacagc aactgcagca cttcaagtac caagtgcaga tgcagctatc gcgatggaag
     9541 aagcatttta ccaattcatc gaagcaaatc cagtaagcgg aacgattgaa gcggacaata
     9601 cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg tgcaagccca caatctggta
     9661 tcaatgcagg ttctttcttg gcattgttct tagataacta tgaattccta ggatctgcta
     9721 aacaatttat ccatgtagca gctgcttatg tccatgaaga tttttatgga gaaaaactag
     9781 gagtcgctta tgaagatgaa aaaatgggca aattgacaat gaatgctgga ctatttgctt
     9841 ttgaagaaaa tggtacagag gaagcgaact tcatcaatat gaatttccgt tttccaaaag
     9901 gtgtaacagt agacggattg caaagtgaca tcgaacaaac agtggggcaa gaaggcgcaa
     9961 cagttactcg aggtgctcgc gtaatggaac cacattacgt accaatggat gatccattgg
    10021 tggctacatt attgcaagtc tatgaagacc acactggaga aaaaggctac gaacaaatca
    10081 tcggtggcgg aacttacgga cgtttgttga aacgtggcgt tgcttatgga gcgatgttcc
    10141 caggatatac tgatacaatg catcaagcaa acgaatttat gagtcttgat gacttgttca
    10201 gagcaacagc aatctatgca gatgccatct atcgtttggc gaaataatta attaaaaaga
    10261 ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt tttttacttt ctactgtggt
    10321 attcatgata aacatgatat agattcaatt catattcttt cttagcgttc gtgacgactg
    10381 tatccaaaat caaccctgtt actaatgaaa gaagtgctaa agtcatcaat ccagtactta
    10441 aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat atattcgcga atcacaggaa
    10501 tccccgtgac caatccaaat aaaaagaaga agatggtcca aatcccaaag aaaagcaaag
    10561 gcttataatc tttaaacatt ttaataatca tcatgatcac tttgaatccg tcagagaatg
    10621 tattcaattt tgattcgctg ccttcaggac gatcacgata atcgattggg atttcaacga
    10681 acttgaattt tttatctaaa gcatggatcg tcaattccgt ttcgatttga aaaccagaac
    10741 tcataatagg aaaacttttg acaaagatac gattgaatcc tcgatagccg gtcatgacat
    10801 caagaatttt tgttttatac attctagtaa ttgtatttct aacaaggtta tttccaaaat
    10861 cgtggaaagg tctcttattc tcatcaaagt aggttccatt tgataggcga tcaccaatga
    10921 ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc atgtacagct tctgcaggat
    10981 aagtatcatc gccatctacc attaaataat agtcggcatc gatatcaaag aacatttggc
    11041 ggatcacatt tccttttcct tgacgaggtt ctttttttac gattgctcca ccttgaacgg
    11101 ccaattcgta tgttcgatcg gtcgaattat tatcatagac ataaatatct gcttctggta
    11161 attctcgttt aaagtctgca atgactgttg aaattgttgc ttcctcattg taacaaggaa
    11221 tcagaacagc aattttttct tgttgttcca tttttcactc tccttaacta tagaaaacaa
    11281 aagctaaatt aaaaatacct cataatcaaa ggaaatgcta gtttttcccg ctaaatatca
    11341 tttatttaaa aagaaaatac tggaatatat gtttggaaag ctctatctaa aaaaccaccc
    11401 actacaaaag aaggacatga tacctctctt ttgaatagtg ggtgcgtatt cgttctctca
    11461 cttttttgac gtattaatct gttgcacctt tttttcttga aatttctaat acggttcctg
    11521 tatgagcatc tgctaaaaat tcataataaa cagtttcgcc atcttctatt cgagaaagac
    11581 ctccccgata agcactcgac cgaacggcga atttcctaat aggttccttt tcaaaagaga
    11641 tccaagaacc ttcgattgca ccttctttta gaaaagatga cttgatattt tcaagtactt
    11701 ggtctgctga caatactttt ttgcgatgat accaaatcgt tgaagctacc ccgctcaata
    11761 atcctagacc gattccaact gctaacccgc ctttaaaata gcttagttcg ctttcttcat
    11821 acatgtggac atacctcctt cttctcactt gttactcctt gtgcttattt taccacactc
    11881 tcatattttc tttgaaataa ataaccgcta ccagaaaaaa taagctataa taaatacgat
    11941 cggtctgcag aaaaaatcat attttatccg cagatcaata taagtctgag aggagtttag
    12001 tctgaaattt agacgaatag catatggaag aaaaaacatt tcaacgaatc aaagagttaa
    12061 cagaattaca aggaacaagc ggttttgaac aagatgttcg cgcttatatg gaaagtcata
    12121 tggaaccgct ggtagatgag ttacagttag atggtttggg cggcattttt ggtctgcgcc
    12181 atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc acatatggat gaagtcggat
    12241 tcatgctgac acaaatccaa gaaaacgggc tttttaaagt cgtacctcta ggcggctgga
    12301 atccttacgt tgtttctgct cagcgtttta cacttaaaac ctctactggt aagaattatc
    12361 catgtatttc ttcttccgtt cccccacatt tattgcgagg aacaagcgga caaaaatcag
    12421 tagaagtaac cgatattttg ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt
    12481 acggtgtcct tccaggagac acaatcgttc cttacgctga aacgatcaaa acagccaacg
    12541 gaaagaatat tatcagtaaa tcatgggaca accgctatgg ctgtacaatg gtattagaag
    12601 ctttagaagc gttgcaaaat gaaacattag gtcatacatt gattgcaggc gcgaatgtcc
    12661 aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa taaattcaag ccagatctgt
    12721 tctttgcagt agactgctct gcagcagatg atacagtgac gaaaaatggc acattcggac
    12781 atttaggaga aggtacattg atgcgtatcc aagatccagg tttgatcatg cttcctcgtt
    12841 tgcgcgagta cttgctggat atcgctgaaa caaacaatat cccttatcaa tactttgttt
    12901 caaaaggcgg tacagatgca ggtgctgctc atacacaaaa cgaaggaatc ccaagtacag
    12961 tgattggcgt agtgggacgt tatatccata cgcatcaaac gatgttcagt attcgcgatt
    13021 ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg tttggataaa tcaacagtga
    13081 atacgatcgt atacggaaaa taatgcgata gtgaaactat aaaatctggc aaaagattgc
    13141 tttttgccag atttttttta taaaaagagg atcaatgttt gttagttcga aagaaaggtg
    13201 tataatttaa ttgaaaaata cataaacaga aagtagagga caaagaatga ttatcccaaa
    13261 tagtttagaa gaattagcag gctacgtaga aactggaaag agtgtatttt tctttactgc
    13321 cggctggtgc ggagactgcc gttttatcaa accgcaaatg cctgaaattg aaaatgactt
    13381 ttctgattgg caatttattg aagtagaccg tgacaagtac atagatgtag ccgctgaatg
    13441 gaacattttt ggtatcccaa gttttgtcgt gatccaagac ggaaaagaat taggtcgtct
    13501 agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg ttcctagaaa gtgtaacaaa
    13561 aggctgacat ctgttaggag gggtcaagat gaatcaagaa tataagaata ttttagtagg
    13621 tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa gcggtagaag tggctcgccg
    13681 taataatggg aaagtatatg tagcaaacgt aatcgatcag caattttata actttatggg
    13741 ctatgcacct attgatcaaa atctagtcga tcagcaaact gaaacagcca aggaaatgat
    13801 cgaagaatgt aaagaatatg ggaaatctgt tgattacaca aatatagaag gaattattgc
    13861 atatggttct gttcgtgaat ccatggcagt caaattgcca aaaaaatatg agatcgatct
    13921 aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc tttatgacag gaagtgtagc
    13981 cagctacgtg atacgtcatt caccttgtga tgtcttgatc gtttctgacg aagaagaaca
    14041 gccaagtaat taagcaagtg gaacttgtac gaccttttta atcaattaac taaaacagta
    14101 aagggactgt gacaaaagtc ttgtcacagt cccttgttac gaataaatgg tgttccagaa
    14161 gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc agtgaatgca attttttgtt
    14221 aagataaatc tcagcaaagg ataatggagg aaagaacatg atttttgctt ataacaaagc
    14281 acacgtcgga gacaccttga tggttattat agccgacgat aaaggaacag aaaacactgt
    14341 tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa
    14401 ctttttccat atctccgatc atctaacaat cgaagggaac ggccaagtaa gcttaacaga
    14461 tgaggaatta gcgaaattga atcgattgat caaagaagca ggattcgaag aacagcttga
    14521 agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa acatgcgtcc cacatcctga
    14581 ttctgaccat ttatcgatta ctgagacaga agtagacaat ggtacagtat tgcaaatcgt
    14641 ttgcggtgca cccaatatcg aagctggaca aaaagttgtc gttgcaaaac caggagctat
    14701 gatgccagat ggaatgatga tctggccagg aactttgcga ggggtagata gctatggaat
    14761 gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca gcaaaaaaag gaatcttagt
    14821 attgcctgag gatgccgtag tcggagaagc atttccaaaa taaagaggtt gtgacaagtc
    14881 tttgtcacaa cctctttttt attaattttc actattttga ttttgtttta atgccgattg
    14941 atcgatcgtc aattcaacag tagttgtttt ttctttcgag ccttcatagt aagtgatttt
    15001 gacagaatct cccacttttt tcttatacaa agcagattga agttctacac cagaagaaac
    15061 ttctttgtca tcgatcttag taatgacgtc gtattgtttc aatccagctt tttcagccgg
    15121 tgtagctgtt tggacatttg tcacgatgac accgttagtt acagatgaag gaatcttcaa
    15181 gatttgttct tgttgctgag tagaaacggc agataagtcg accattgtga tacctagagc
    15241 tggacgtgtc acttttccgt ctttttccag ttgattgatg atattcacta catcgttact
    15301 tggaatagca aatcccattc cttctacgct tacattagaa gtagattcag aagtacttgc
    15361 gatcttactt gagttgatac cgataacttg tccttcgata ttaactaaag gaccaccaga
    15421 atttccaggg ttgattgctg catctgtttg gatcgcattg atattgactg tttcgttcga
    15481 ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg attcctgaag tgacagaatt
    15541 tgcatattca gatcctagtg gggaaccaat cgcaatcgca ggctcaccaa ccttcagagc
    15601 acttgagtca ccaaaagagg caactgtttc gactttgtct gaattgatct tcaacaccgc
    15661 taaatctgaa taagcatctg ttccaactaa ttcagctttt accttcgtac cgtctttcat
    15721 caagacttca agaccttgct ggccatctac tacgtgatta ttcgtgacta cataagcagt
    15781 attcccgtct tttttgtaga tcacgccgct tccttcacta taagcttcca aattactatc
    15841 atctgagctg ctactttctt cttgctgccc gaatagtccg ccaaaaccgc ttgattggtt
    15901 ttgactttgt aagttgataa cagaaacgac agcgtcttga actttatcta ccgctttggt
    15961 gatatcagaa tctacattga ctttgatatt ttccacaact gtttcaccgg cagaattctg
    16021 attgccgctt gtagcagtag aactattgtt gccagtgccc atcgctgcat aaaaaatgcc
    16081 agctgtcagt agtcctccaa cgatccctcc aacaagcccc agacctaact tacgccagag
    16141 gctattattt ttactttttt tcatctttgg tgtcacattt tttctatcca tgtgttcctc
    16201 ctccaattac attcatttat ctactgttag tatagtctta tttgttgact ttagtctact
    16261 ttgagactgt gaacttttta tgaaaaactt taggaaaaat cattgttttc agatgaaaat
    16321 atttgatttt ctttttccac agatactgtg taaaactacg cggataggat tggaaaaata
    16381 agggataaaa catatccaca gcctgtgtat aaagtggata aattcttata aaacgtatgt
    16441 ttgcctaatg aatgtgatga aataaggtaa aataacactg ttttctgtgg ataaacctgt
    16501 ggatgttgtg cataactgtt ggggaaatct ttttaacaaa aatagccaaa ataaacatat
    16561 agacgaaaaa attgattata ataaacaaag tgagcatgaa gaccaaagga gagaaaacat
    16621 gaatatcaaa attatctcag taggaaaatt aaaagaaaaa tacctgatcc aaggtatcaa
    16681 tgagtatgtc aaacggctga atgcctatgc aaaaatcgaa ttgatcgaag ttcctgacga
    16741 aaaagccccg gagaatttaa gcgaagcgca aatgagacaa gtaaaagaaa aagaagggga
    16801 acggatctta gcgaaaatca aagaacaaga atacgtctat gccttagcaa ttgaagggaa
    16861 aaatcctaca agtgaatctt ttgctaaaca aatcgaccaa ttaggtatcc aaggaaaaag
    16921 ccaccttgtt tttgttatcg gcggctcttt aggactcagc gaagccgtca tgaaaagaag
    16981 caatgctcag atctcttttg gaaaaatgac gtatccacat caattgatgc gtctgatcct
    17041 tgtcgagcaa atctaccgtg ccttccggat caatgcgggg gcgccttatc ataagtgatg
    17101 cggtttttat cattgatcct aaaaatctta gcaagacaaa aagtacgtaa gaaagtaaag
    17161 actaaatact tcggaggaac aaatgaataa caatgatagg ttattgcgat tgcgttatgc
    17221 gattgatttg aaagatacgg atttgattaa agcttttgag ctaggcggtg taccactgac
    17281 taaagaagaa gctcaggcgg tactgaccaa agtacaggat aagaacaaag ataatgcaga
    17341 aaataatgtg tatgaaaaaa ccatcaacaa ccaagttttt gacgcattcc taaatggcct
    17401 gattctcttg gcgcgtggtc cgcaaaaaga taagccgata gtagacacgc cgcaaaaaag
    17461 caaggaaatc aaatatatca acaatgtgtt gctaaaaaaa ttaaaaatcg ccttgtctat
    17521 gaccactgat gatattttag acgtttttgc tgaggcagaa atctatccat caaaaggcga
    17581 aatcggggca tttttgagaa aagaaggcca acgaaatttt aagccttgcg gagataaata
    17641 tatgcgtaat tttctcaagg ggttaggaat ttataatcgg agaaaagtat aaagaaattt
    17701 catgagaaat caaaacaatg gttgttttga tgagagctag tttttgtatg agattaaaat
    17761 attatcttaa tcgaggagct ggattggttt tatgaatgat gaatataaga atgatgaaga
    17821 taagatgctt tttgaagaga tagagaatcg atgtaggctc aattttgagc ttcggggaaa
    17881 gatgtcttta atacaacaaa aaaagtattt agcgaataaa tcagagttta ctctgggaca
    17941 tgttgaaaaa ttaatctcgg attggattag tagtagaagc gaatttacaa aaataaaaca
    18001 gccgattaaa tttgacatga aaaaactttt gttaaataag agtgagatag gtaatagaga
    18061 tcaatatatt agagctaaag ggcaagaaat aattgattca ttaggtgaaa tgaggtcata
    18121 caattatttg tatgtaacac atagagctga tggaatggtc attactgtgg ggaaatcttc
    18181 ttctaatgat atttttttag atggagatct tttttatcaa ctcaatacaa atcacttgtc
    18241 tggcactgaa aatataattt taaggacgga atatggcaat gaaatatttg caaaatatga
    18301 tgagatttta aaaaattatt tggactgggc ttggataatt ccggttgagt caggagatgc
    18361 taagaaactg gagagattat tgggcgatga actaattaat aaaaaagtac caattttaaa
    18421 ttattattcc catagacaat aaaaggctat ttttcatgat aatactagtt ataactgtaa
    18481 aaatggaaga aaatctggga aagaagtttt taaataagtg tattgttcga ttttaataaa
    18541 taataagcaa taccatccgt ctgtgttgcg caacatcgta ctacaatgaa tattttttca
    18601 caaaaagaaa atcatccatc gaatgaatgg aaggttttag tatacacgag gttaatgccc
    18661 cctcttttat aatcagagga gtgaggtttt ttctatgaat gtttctattt tcttattgct
    18721 agtctagttt ctcttacgat gaaattggga ttctattcca gtttttattg atttgctgat
    18781 aaaataactt gaatggtgga tataaacatg ataaataaga gaagaataac aaaacaaggt
    18841 tgtgaaatga ttctcaaatg aatataatag aaagaaaaag gaggaagaat caatgaaaat
    18901 agcaattgca ggcgctggcg caatgggaag tcgctttggg ttaatgcttc accaaagtgg
    18961 gaacgaagta ttattaatcg atggctgggc agaacatgtc caacaaatca aagagcatgg
    19021 attacaagca aattttaatg gtaaagaggt agaagcaaaa ctaccaatcg ttcttcaatc
    19081 cgaagtagaa aaagaagatc aagttgatct gattattcta tttaccaaag cgatgcagct
    19141 ggaaaaaatg ctgcaggata tccaatcatt aatcaaaaaa gatacagagg tcttatgtct
    19201 attaaatggt atcgggcatg aagatattat tgagaaattt gtaccaatgg aaaatatcta
    19261 tattggaaat accatgtgga cggcaggtct tgaaggtcct ggtcaggtca aattatttgg
    19321 aagtggttca gtagaattac aaaatttagg tgatggaaaa gaagcagctg caaaaaaatt
    19381 agcagacaaa ttgtctgaat caggattaaa tgctcatttt tctgacaata ttcactattc
    19441 tatttatcgc aaagcatgtg tgaacggaac aatgaacgga ctatgcacaa tcttagacgt
    19501 taatatggct gagctaggaa aaacatcgac tgctcataaa atggtggcga cgattgtcaa
    19561 tgagtttgcc aaagtagcag cagtagagaa gattgaacta gatgtcccag aagtcattgc
    19621 acattgtgaa tcttgttttg acccggaaac aattgggtta cattatcctt caatgtatca
    19681 agacttgatt aagaaccatc gattaacaga aatcgattat atcaatggcg caatttctag
    19741 aaaagggaag aaatatggtg ttgcgacgcc ttattgtgat ttcttgactg agcttgtcca
    19801 tgcaaaagaa gatagtttga acgtaaaata aaggagagat tgaatggaaa cttcacaaaa
    19861 aatatcaccg aaaatttttc tgaataaagt cctcgctggt acagcaacag gaatcattat
    19921 tggattgatt cctaatgcag tattaggtgc cattttaaaa tattttagtg cgtatccctt
    19981 tgcggtaacg atcgcacagt tagcagttat ttttcaacta gcaacacctt tgatcattgg
    20041 gtttgattgc cttacaattt ggatttgatc cgatgagaat gatggttgta gccggagctt
    20101 cttttgttgg ttctggtgta gtcaaattca cacctgattt gggtgaaacg ggtgtttacg
    20161 ttggtgctgg tacaggcgat attattaata cgatgctgac agcatcgatt gctgtcgggc
    20221 tgattttact gattggaaat agttttgggt cagtgtcgat tgtgttaaca ccgattgttg
    20281 taggtgtagg tgcaggttta cttggttttt atttattccc ttttgtaaca gcaattactt
    20341 ctgcaattgg atcattgatt aataacttca ctaccttaca accgctgtta atgtctatct
    20401 tgattggttg ttcctttgcc tttttgatta tctctcctat ttcaacagta gcaatcggaa
    20461 tggcaatcca actagatggt gtttctgctg gtgcggctgc gatgggcgta gctgcaacca
    20521 cttttgtttt agtagtgaac tcttggaagg ttaataagtc aggtgtgaca attgcgattg
    20581 ctttaggcgc tatgaagatg atgatgccta atctatttag aaaacctgtg attcttattc
    20641 cttgtttagt tagtgcagtg attacagcta ttcctgtcgc tctattttct atttcaggta
    20701 cgcctgcttc tgctggtttc ggcgtggtcg gacttgttgg tccgttggct tctttagatg
    20761 cagggctaaa tggctttctt gttgttcttt gttggttagt catccctgtt tttgcggcac
    20821 taattactca atttttatgc gagaaagtat ttaaactgta tgatcgagaa gaagtcttca
    20881 aatttttagg ctagtagatt ttactaagaa attttcaatc aatggatgcg taagtgagat
    20941 gtcaacaaca tctgacttac gtttttttgc ctttttgagt aggagattga tgctgacaac
    21001 tgaactcttc agtgccattc gattttttaa aatgtagaag aaactcacct ttatgttatt
    21061 gattgttcaa aaggcaacta gaataaaaat atgtgttaaa atgagaaaaa atttgattgt
    21121 tggatgtatc gaataatcac taaaaagtag gagaagaaaa tgattttttt gcaaaaatat
    21181 ggtttttatt ttttattatt aggtgtgatt agtgatttat cgacaccgta tatccttggc
    21241 cttttttatc ccaaactgaa tcaaatgact accgtaatta gtgtgtttgg tgacgtagat
    21301 agtccagtca gacgtgcttt cttggtctgg tcagttgtat ctggactttt ttttgttctt
    21361 tcgttgcctg cgttgtatca cctatttatt ggaacatcaa aaacacttgc aatacttgct
    21421 gttgcaacag ttggtttata tgggataggt gattgtattt ttactgggtt attcagtatc
    21481 aatacgaatg aaagctcttg gaatctatcg acttggatcc ataatatagg ttctgggcta
    21541 ggctatgcag gctttctact tttcccattg ttacttgttc tgctttatcg tcaaagtggt
    21601 caaggtacgc ttagccattt ttatcttgtt ttaacagtta ttagtttact aattgcaggt
    21661 ctttacagat tagcacggat tccgtcgatt agtcagtttg ccttctttaa gcaattgggc
    21721 ttctttcaac gcttaagttt tttctttaac tatttgccga tgatttgttt aagttttttt
    21781 caactacgaa agtagaaaaa gcagtgtgaa gctgattaag taaaagaatt tctctatggg
    21841 taaccattcg tgtggatgtg ttcgactaag aatagagatg aggagagcga aaagcaagtt
    21901 agtcttaact tgttatgcgt tgtgctaaat ttcaggtagt cattttttga atgaataaga
    21961 agaaaacttg aaagagggtg acttcatgtt agatgtggag cagatggaaa tagaccttta
    22021 taatattatt ttaaatcgaa atacaagaga atgggaaaga acacaatttg aagaggcaaa
    22081 agaagaccta gcaaaaatgt cgataaagca ggtgatagaa aaactagagg tcaacttgcg
    22141 tccgctagca ctacgagata atttgacacc agatgcgatg gatttttatt taagaatcac
    22201 aggggagtca gcgagtgcaa aggcttatga ttatgccaaa cataagataa cttcaccctt
    22261 ataccaagaa cgggcgattt ttgccggtgg ctgtttctgg tgtatggttg agccttttga
    22321 aacaaaacca ggtgtaatct cagtgttatc tggttataca ggtggagaga ccaaaaaacc
    22381 aaattacgat caagtcagtg gtggatatac tgggcatgtt gaagctgttg agatcatatt
    22441 tgatacacgt caaataacct atgaagagct gttggagatt tattggcaaa tttcagaccc
    22501 tacagatgcc tttggtcaat ttcaagatcg ggggaaacaa tatcgttcaa ttctatttgt
    22561 tgttaatgaa aagcaaaggc aattggctga aaaatcaaga gaacaactga ttcaatctaa
    22621 ccgatttagc cgtcctatcg ttacagagat tcgccaagca aagacttttt ggccagctga
    22681 aaatcatcat caacaatttt atcagaagca acctaaaaga tataaaaaga tcaagcattc
    22741 gcatcagctt catttatttt ttcagcgaca tagatagagg aaggttgtgg cgcaaagcgt
    22801 tcagctccga caaaataatg agcaactgac aaaaatagct tttcatattt ttggacagtt
    22861 atgaattatt tcgaggagtt gctttgagaa caccgaggaa aaggttgtgg cgcaaagcat
    22921 tcagcttcaa gtcgcaagga aacaggaggt atgacaagtc ttttgtcaca actcctgttt
    22981 ttttactagc ctttatttcc atgtgaaaga gaattcgaat tgtagaatct tgatttttct
    23041 atggaatttt gtagagaatc atcttgataa ttaatagaca cctaatcgaa ttccacatta
    23101 atgatgaact aaattccgta taaaagcgca aacaataact a
//
LOCUS       DACBZN010000036        22855 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.036, whole genome shotgun sequence.
ACCESSION   DACBZN010000036 DACBZN010000000
VERSION     DACBZN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.036"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(104..1336)
                     /locus_tag="IVD64_001382"
     CDS             complement(104..1336)
                     /locus_tag="IVD64_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6148641.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IVD64_001383"
     CDS             1591..2160
                     /locus_tag="IVD64_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6148642.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IVD64_001384"
     CDS             complement(2338..2778)
                     /locus_tag="IVD64_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP6148643.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IVD64_001385"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IVD64_001385"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP6148644.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IVD64_001386"
     CDS             3732..4655
                     /locus_tag="IVD64_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP6148645.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IVD64_001387"
     CDS             4731..5870
                     /locus_tag="IVD64_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP6148646.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IVD64_001388"
     CDS             5863..6663
                     /locus_tag="IVD64_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6148647.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IVD64_001389"
     CDS             6663..7490
                     /locus_tag="IVD64_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6148648.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IVD64_001390"
     CDS             7468..8202
                     /locus_tag="IVD64_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6148649.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IVD64_001391"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IVD64_001391"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP6148650.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IVD64_001392"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IVD64_001392"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP6148651.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IVD64_001393"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IVD64_001393"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP6148652.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IVD64_001394"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IVD64_001394"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP6148653.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IVD64_001395"
     CDS             11787..13820
                     /locus_tag="IVD64_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148654.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IVD64_001396"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IVD64_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IVD64_001397"
     CDS             15353..16159
                     /locus_tag="IVD64_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148655.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IVD64_001398"
     CDS             16171..17391
                     /locus_tag="IVD64_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148656.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IVD64_001399"
     CDS             17381..18967
                     /locus_tag="IVD64_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6148657.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IVD64_001400"
     CDS             19006..21144
                     /locus_tag="IVD64_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6148658.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="IVD64_001401"
     CDS             complement(21512..22495)
                     /locus_tag="IVD64_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP6148659.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4711 g         7069 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttg
//
LOCUS       DACBZN010000037        22552 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.037, whole genome shotgun sequence.
ACCESSION   DACBZN010000037 DACBZN010000000
VERSION     DACBZN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.037"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            272..2743
                     /locus_tag="IVD64_001402"
     CDS             272..2743
                     /locus_tag="IVD64_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6148660.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2846..4582)
                     /locus_tag="IVD64_001403"
     CDS             complement(2846..4582)
                     /locus_tag="IVD64_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP6148661.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4579..5283)
                     /locus_tag="IVD64_001404"
     CDS             complement(4579..5283)
                     /locus_tag="IVD64_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6148662.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDLHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5414..5917
                     /locus_tag="IVD64_001405"
     CDS             5414..5917
                     /locus_tag="IVD64_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP6148663.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5877..6215
                     /locus_tag="IVD64_001406"
     CDS             5877..6215
                     /locus_tag="IVD64_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP6148664.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6236..7654
                     /gene="ffh"
                     /locus_tag="IVD64_001407"
     CDS             6236..7654
                     /gene="ffh"
                     /locus_tag="IVD64_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP6148665.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7766..8344
                     /locus_tag="IVD64_001408"
     CDS             7766..8344
                     /locus_tag="IVD64_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP6148666.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8348..8869
                     /locus_tag="IVD64_001409"
     CDS             8348..8869
                     /locus_tag="IVD64_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6148667.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8948..9502)
                     /locus_tag="IVD64_001410"
     CDS             complement(8948..9502)
                     /locus_tag="IVD64_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6148668.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9755..10030
                     /gene="rpsP"
                     /locus_tag="IVD64_001411"
     CDS             9755..10030
                     /gene="rpsP"
                     /locus_tag="IVD64_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP6148669.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10042..10293
                     /locus_tag="IVD64_001412"
     CDS             10042..10293
                     /locus_tag="IVD64_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6148670.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10418..11209
                     /locus_tag="IVD64_001413"
     CDS             10418..11209
                     /locus_tag="IVD64_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148671.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11379..11900
                     /gene="rimM"
                     /locus_tag="IVD64_001414"
     CDS             11379..11900
                     /gene="rimM"
                     /locus_tag="IVD64_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP6148672.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11890..12651
                     /gene="trmD"
                     /locus_tag="IVD64_001415"
     CDS             11890..12651
                     /gene="trmD"
                     /locus_tag="IVD64_001415"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP6148673.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12787..13134
                     /gene="rplS"
                     /locus_tag="IVD64_001416"
     CDS             12787..13134
                     /gene="rplS"
                     /locus_tag="IVD64_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP6148674.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13507..14169
                     /locus_tag="IVD64_001417"
     CDS             13507..14169
                     /locus_tag="IVD64_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148675.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14248..17475
                     /gene="fss3"
                     /locus_tag="IVD64_001418"
     CDS             14248..17475
                     /gene="fss3"
                     /locus_tag="IVD64_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6148676.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17589..17903
                     /locus_tag="IVD64_001419"
     CDS             17589..17903
                     /locus_tag="IVD64_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6148677.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17916..18290
                     /locus_tag="IVD64_001420"
     CDS             17916..18290
                     /locus_tag="IVD64_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6148678.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18300..18644
                     /locus_tag="IVD64_001421"
     CDS             18300..18644
                     /locus_tag="IVD64_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6148679.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18715..19860
                     /locus_tag="IVD64_001422"
     CDS             18715..19860
                     /locus_tag="IVD64_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6148680.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            20575..22434
                     /gene="ltrA"
                     /locus_tag="IVD64_001423"
     CDS             20575..22434
                     /gene="ltrA"
                     /locus_tag="IVD64_001423"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6148681.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         7732 a         3969 c         4681 g         6170 t
ORIGIN      
        1 gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaccaac
      121 acaaaatatt atagaaccct ttttttttgc tatatgacag gaatgaaagg tgtttgcaag
      181 cttaatgtga ggaacacttt ccatatctgt tctatactgg ataggagaag tttggaaata
      241 tccagaaaac gaaatcaatc gaggtagcca catgaagaat aaatggataa atcgaagtaa
      301 aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc cgtctttttc catcgtataa
      361 agaaggatta acggcagatc aagtcaagga acgaatagaa aaaggtgcag caaataattc
      421 agtcgatccc acattcaaaa caaatcagca aatcgtattg gaaaacatat ttacgtattt
      481 caatttgatc tttcttatct tagccatttt gctttgttta gtagaatcct ataagaactt
      541 gactttttta ccagttatca tagccaatac aggaatcgcc atttttcagg aaattcgatc
      601 caaaaagatc ttggacgaac taaatgtttt aaatacagca aaagtcaatg tcatacgaga
      661 cgggatagaa caaacaatag atatagaaga ccttgtaata gatgacattg ttattttaga
      721 gactggatat caaattccag cagatgcaga ggtcgtggaa gggaaactgc aagttaatga
      781 ggctttattg acaggcgaag cagatgaaat aaccaaagaa ataggtgatc cattaatgtc
      841 cggcagtttc attgtttccg gaaaagctta cgcccgatta gacaaagttg gagcagattc
      901 gtatatttcc cagctgacag tcaaagccaa gacaatggga gaaggagaac agtcggagat
      961 gattgcttct cttaatcaat tgatcaaatg gattgggatc atcattattc ctatcggtat
     1021 cctgcttttt tctcaaagtt acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc
     1081 aatggaagca gcattgatcg gtatgatacc tgaaggactg tacttgctga cgacgattgc
     1141 attagcatta agtgcgacta aactagcaaa gcaaagagtt cttttacaca atatgaaaag
     1201 cattgagaca ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga
     1261 aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa caagaaataa tcagtcagtc
     1321 agatcaattg gaagcatgca ttgctgacta tgcaaaagcg atggcaggag acaatgcaac
     1381 aatggaagct gttaaagcat attttacaaa aaccactgat caatcctatc accatgttat
     1441 tcctttttct tctgtccaaa aattcagcag tgtagcgttg gcagacaagg tgtatgtttt
     1501 aggagcgccc gaaatggttt tgcgcgatca attgccggaa tatgcggaag agtttgtttc
     1561 tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga
     1621 accagtattg acagaagcag tcacaccatt aggctatatt ctgattgcta atcctatcag
     1681 aaaagaagca agagccacat ttgattactt caaaaaacag caagtagcca tcaaagtgat
     1741 ttctggagat aatccgctca ccgtatccaa tgtagctgtc caagcaggga tttcacatgc
     1801 tgatcagtac gttgatgcat ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa
     1861 atatacagtt tttggacgcg tcaagccaga acaaaagaaa atcttcgttc aattgataaa
     1921 gcgtaacaat acagtagcta tgacaggtga tggcgtgaat gatattctag ccatgaaaga
     1981 agcagactgc agtatagcga tggcttccgg aaatgaagcc gccatgcagg cctcacaagt
     2041 ggtcttactt gattctgact tttcacgcat gccagaagtt gttgcagaag gacggcgcgt
     2101 tgtgaacaat attgagcgat cagccagctt gtttttagtg aagaatattt tctccttttt
     2161 gctctcactg ttttcagtga tctttgctct gacctatcct ttagaaccat cacaaatcac
     2221 attgattagt ttgtttacta tcggacttcc atctttcttg cttgctctag aagaaaacaa
     2281 aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc caggtggact
     2341 aacagatatg atcgtagtcg gtgcgttggt gatctgcggg gtgactttga atctaaataa
     2401 aacagatgtt tctacagcat ctaccatgct tttgattgca gtagggttcc tcgttctcta
     2461 taaaatctgt tcaccgctga ataaattcag aagtcagatc attcttttct gtgctagcgg
     2521 taactttttt tcggtcatct ttttgcataa gctgttttca atcaccggta tctcagctgt
     2581 atcacttttg ctgttgagca ttctattttt ctcggcagat tcgattttta ggcatttgac
     2641 cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg
     2701 cgtagctaaa aaatttctat cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa
     2761 aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc aggatgagtc atccaacttt
     2821 aaaacgcacg agcagttgct cttttttaca ttttcggaat ggtaacggtg aaagtcgaac
     2881 ctgttccaag atgactgtct acagtaatcg taccattaag gagttgcacg tagtctttta
     2941 caatcgctaa ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt
     3001 aaaaacgctc aaagatccgt tcctgatcct caatgtcgat acctatacca aagtcttgga
     3061 cagaaaacga tagatcatct gttgcctgat agcggataat aatctcgctg tcgctttttg
     3121 aatattggac agcattttca attagatttt tgataattgg ataaaggata tctgttttag
     3181 tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg
     3241 gctgataatt ttgaacgatt tgctggaaat aaggttctgt ataaattgtc tggatctcgt
     3301 aagaattctc gccatctttt gacagctgga tgatttcccg aatcagttta tctaaccgga
     3361 tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac
     3421 catccagtaa agtctcggta aaaccaatca gtgaagtcac aggagttttt aattcgtgag
     3481 agacgttgcc aacgaaatcc ttttgtagct tttctaaacg tcgcactttc gttaagtcat
     3541 acgccacccc tagaatctgt ccggtacctt ctgaattagt aaagtagcgc aaactcaagt
     3601 ctaatgtctg ccgaccttct gtaagggtga tctcctgatg gatcagcgga tgctcaggag
     3661 tcacttgatg aatcagctga atgagttttg gctcttgaat gacttctgtg taccgctgtt
     3721 caggttgata gcttaccacg tttaaatgta gctgcatctt tggattaagc agtagtaaac
     3781 gagaatcagc atcatcaatc aggaatacgc caatcatcag ttcgttcagt aacgtatata
     3841 attgctcttc tgtggtagta cacgcccgat acatcttact catttgttcg ctcaacgcat
     3901 taatcgtctg atacaattct tcccattgat cgggtgtttg catgattact tccgcctgat
     3961 taggattttg caccattttt tttagtacgg gcagcacggt tttcaatggc tggttttttt
     4021 gatagatcag ataatagatc attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa
     4081 agacccaacg gcgaaaactc tcagttctcg gtaaaaaacc gcttgtcggt tctgcgatac
     4141 gtaaaatggc ttctaactgg ccactttttt tgactggtaa tgcgacatat aaaagatcct
     4201 cattcaaagt agtacttttg cgcaaagcgc tacctaggtt cccaccattc aatacggctt
     4261 tgatttcggg acgggagttt cgctgttcat tcaatgtttc attgtttgtg tcaaataaga
     4321 tctttccagt agcatccatt aaagtaatcc gttcgttcga ctgatggacg aattcttcca
     4381 aagcagtttt gttttgcgct gcttggaggt tttcgacatc taattgccgg atcagcagtt
     4441 gtgctttttt tgtcaggtag ctttcttgct gttccacgat ctgctgttga aaataatggg
     4501 aaatcaattg ccagccacca aaaaacaaac taactaaaag aataagaaag aagagacgtt
     4561 cataaatttt tttccatttc atcgtttggg ttcctgaaac ttatagccaa atcctctaat
     4621 agtcaccaag tactttgggt gttttggatc tcgctcgatt ttttctcgca aatgactaac
     4681 gtgtaaatct acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa
     4741 tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa taaacaagta attcaaattc
     4801 tttcggtgtt agttcaatca gatggtcgtc tacggtcact tgataattgg tcaaatcagc
     4861 agtgatttgt ccaatcgata gatattcagg ctcagcttca tcttttgatt gcttacgagg
     4921 ttcaattcgt ctgaatattg ctttcatacg ggctaatact tcgcgaggac taaatggttt
     4981 ggtcaaatag tcgtcagcac ctatttctag gccaatgatc cgatccactt ggtcatcttt
     5041 agccgtcagc atcaaaatcg gtgtttcatt tttttcttga cgcagccgct gagtgatctc
     5101 catgccatcc atcgttggaa gcatcacatc taaaatgatg aaatcaaatg attgttctaa
     5161 tgctaattct aatccttcgc ctccatctgt ggcactggtt accttgtagc cttctttttc
     5221 caaattgaac gtcaagagcg ttacaataga aggttcatca tcaacaacta gtattttttt
     5281 cattcaaatc tcctttaaat aggtttcatt ctgtttatat tttagcatag aatcagtaca
     5341 aagggctatt ttacaaaaga aataaatggt ataataggaa ggcactttgc tgaatagaaa
     5401 ggaagagcag tcaatgattg gaatcagtgc atgtttaggc ggtctggcct gtcgctatga
     5461 tggacagaaa aaagaagtac cagaattgat ggaactagtt gaaactggag aagctgtcat
     5521 ggtctgtcca gaagtgatcg gcggattacc aattcctaga gatcccgctg aaatcattgg
     5581 tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa gcggcgaaga
     5641 tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat caaaaattgc aggaaaaaca
     5701 cattgatcta gtcatcatga aggaaaaaag cccttcatgc ggaacccatt cgatttatga
     5761 cggaagtttt tccggaataa aaaaaaatgg tgccggagtg gctactgctt attttattca
     5821 gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa actagaggtg agcagaatgg
     5881 aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt ttattcgacc ttattgacag
     5941 aaaaacaaat gaactacatg gaattatatt atgcagatga tttttcatta ggtgagatcg
     6001 cggaagaata cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg acaagcaaga
     6061 tcttggagga ttatgaaaaa aaactccatc ttttttctaa ttatattgtt cgagaacagg
     6121 tattggaaaa tatgactgca tatattacag ataagtaccc ggaagataaa aaactacttg
     6181 aatatgtaca gcaaattcaa gcaatcgaag aataaataaa aggaatgaag aaaatatggc
     6241 atttgaaagc ttaactgaac gtctccagca ggcgatggga aaaataagaa aaaaaggaaa
     6301 agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct tactagaagc
     6361 cgatgttaac ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg
     6421 ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga
     6481 attgacgatc acacttggtt ctgaaacagc agagttaaat aaatcaccaa aaataccaac
     6541 tgttatcatg atggctggtc ttcaaggggc aggtaaaacg acctttactg gtaaattagc
     6601 caattattta aagaaaaatg aaaatgctcg tccgctccta atcgcagggg acgtatatcg
     6661 accagctgcg atcgaccaat tgaaagtatt aggtcaacag ctagacgttc ctgtatttga
     6721 tatgggaact gaagtcagcc cggtagagat cgtccgtcaa gggatggagt tagcaaaaga
     6781 aaagaaaaac gactatgtat tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt
     6841 gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt tgcttgttgt
     6901 cgatgccatg actggtcaag atgccgtcaa cgtagctgat agcttcaacc agcaattagg
     6961 tattacaggg gtcgtcatca ctaaacttga tggggatact cgaggcgggg cagcactatc
     7021 gatccgctca gtgacaggcg caccaatcaa attcatcggt tctggtgaaa aattgactga
     7081 cttagaagta ttccacccag atcgtatggc tagccgtatc cttggtatgg gggatatgct
     7141 gacattgatt gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag aacttgcaca
     7201 gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt gaacagcttg atcaagtgat
     7261 gggtatgggc ccactggaag atctaatcaa aatgatccca ggaatgaatc aagtccctgg
     7321 aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga tggtttattc
     7381 tatgacgcca gcagaaagag caaatcctga tctgttgaac ccaagccgtc gtcgccgaat
     7441 tgctgccggt tctggaaaca gtgttgttga agtgaatcga atgatcaaac aattcaagga
     7501 atcaagaaaa atgatgcagc aaatgtccaa aggggatatg aacatcccag gtatggacca
     7561 aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa
     7621 gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct tcggtacaga
     7681 ctgaaaatgt ctactgccgg agcttttcta tcagcaattt ttattcttta ctaatacaat
     7741 tactctaaac gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa cagaaagtat
     7801 gcggcaatat catgatactg tctggggtgt tcctgaatat gatgaccaaa agctgttccg
     7861 aaaattgatg cttgatatta atcaagccgg attgagctgg cagacgattt taaataaaag
     7921 tgaagcattc gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa attatggaga
     7981 aacaaaaatc agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga
     8041 agcagccgtc caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc
     8101 agagtatctt tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc attcacaaga
     8161 agaagtccca acaacaagtc ctctttcaga tgaattagca aaagatctaa agaaaagagg
     8221 atttaaattt atcggaagca caacaatcta tgctttttta gaagctgtcg ggatcatcaa
     8281 tgatcacctt gacagttgtt tccgcaaaga agaggtcgta cgtatcggca gaaagggcga
     8341 ataaagcatg cgaaaaatca tattttatgg tgctataagt ttagatggct atttggcaac
     8401 gaaaacggat gatcttcaat ggctttttga cactccaaca ggtaagcaaa ctacttatga
     8461 agctttttat gaaacaatcg atacgaccat catgggcaga aaaacttatc aggaagcaaa
     8521 aaaatatttg gagaccgaag caatttatcc agagaaaaag aatttcgttt tttccaccca
     8581 cgcacaatta caattgccag atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa
     8641 aaatctacaa atgacagaag gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa
     8701 accgctattg gaacatcaaa tgattgatga atggtatatc cagattaccc ctgtattatt
     8761 aggggacggg attcctttat tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga
     8821 cacgacacga ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga agtaaataaa
     8881 ataaaaaagg caactatatg cagaaatgtc ctgtacatag ttgccttttt tattaccgtt
     8941 atgcttttta ctctgttaat ttgttagaaa aatctagata cgcatctaaa atcgtttgga
     9001 agaaacttaa tgttccttca acgatattac cattttcatc aattagattc gcaacgtttc
     9061 cgatatatgc ttccggttgc tgcaaggttg gcacattcaa aaagacaagt gattggcgta
     9121 gatggtggtt tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg actaaacctg
     9181 gtttatgatc ccaaacactg tgtccataag gtcttgagcc tacatctaaa gcatttttca
     9241 atactgctgg cacagaacgg ttgtattcag gagtaacgaa aacgacaccg tctagtcccc
     9301 tcacttcctc acgaaaacga gtccattcag caggagcctg ttctggagtt tcgagatcct
     9361 cattgtaaaa tggcagatca tcgattttta caaatacact ttcgtaccct tcaggaagca
     9421 atttcgcaaa agcttctgct acttttttgt tgtaagaatc ttttcttaaa ctaccaataa
     9481 aaaatccaat tttttttgtc atacgatttt tcctccttca aactatctat aataatctta
     9541 caaaagataa gtaactggag caaataaaaa cacttactga caaaagctac atagaagata
     9601 gattacttat ttttaacaaa aaagaagtct aaaatcttct attttctaaa aaaaatcgaa
     9661 tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt tacactacat
     9721 cttgtgaata aaacaatgga ggtggaaata aataatggca gttaaaatcc gtttaaaacg
     9781 tatgggttct aaaaagagtc cgttttaccg tatcgtcgta gctgattcac gttctcctcg
     9841 tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt
     9901 agttttaaaa gaagacttag ttcttgactg gttatcaaaa ggtgcacaac cttctgacac
     9961 tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc
    10021 aaagaaataa ggtgaccgga tatgaaagat ttaagcgact tagtcttaac aatcgttcgt
    10081 ccgctagtca cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa agacttttac
    10141 gagtacaatc taacagtggc tcctgaagat attggccgta tcattggaaa acaagggcgt
    10201 gttgctaaag caatccgcac aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg
    10261 cgtttgaata tcatcgatga taaggaaaag taaataaacg tgctgtactc gaattttcga
    10321 gtgcagcttt tttgtttttt ttattaattg aagaaatgat tatttggcaa aacaagcaag
    10381 aaagcgtatc cttataggag gagggagtga acagccaatg agaaagaaaa tgttatttat
    10441 tccatttgct ctattgttaa gcgcttgtag ttcggttact caagaatcag acaatacaca
    10501 tctatcaagc agtatatctg aaactgttac cagtccagaa aaaagtagta ccaaaacggc
    10561 ttccaccact gaacaaacaa tggctacctc aaataatgag aaaaagactg ctttagacca
    10621 gttgaaagaa caacagccaa atgttcccat gcctctagat gtaccagtct cttcaggcta
    10681 tctcaatatc gcagcgacac atacaaagca aggttattct attttatatt acagaacaga
    10741 tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa acgcctattg caacctatct
    10801 atatcaatat gggtttgctt ctagtcaaga aacaatccaa gttttgcagc catttgagat
    10861 cgacacaaat ggtcagcaag tggacttagg ttctcgaatc acagggtatc agcaaggtgc
    10921 agcaggttca agttttcttg aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa
    10981 taatatagaa ggccaagatc ccttactatt agctaaagaa atcgtagctt atttggaaga
    11041 aaacagtcta ccagcacctg aacaatttgg taaaattact gttgatatgg gtgatacgac
    11101 gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat gcctatacga tcactcatca
    11161 agatcctatg tctgcgttga agatggcagt ttctatgaag aggttgtgaa atcagcgcgc
    11221 tgatctgagc ccatcattta actccaagtc acaggaagta atcaaaaata gcacgccgca
    11281 tttttgcata attttggctt aataccttaa aagaaaacta gaattgcctc ttcacacatg
    11341 ctaaaataaa acagacaaaa aagaaatggg gatataagtt gactgaatac ttgaatgtag
    11401 gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg tgttatctca acaacggatt
    11461 tcccagaaga acgttacaaa aaaggaacgg tgctaacact attccaagac ggaaaagcac
    11521 ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa ttttgactta ttgagttttg
    11581 aaggacatcc ttctatcaat gatgtggaaa aatatcggga tggcatatta aaagtgtcaa
    11641 aagacaactc tgtagatcta gctgaaaatg aattctatta ccatgagatc atcggtctgc
    11701 atgtattaga agatggaact gagttaggta aagtaaaaga aatcttgtca ccaggtgcca
    11761 atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca tatatcgctt
    11821 ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg tgtagaaatt ccagaaggac
    11881 tgattgacga tgaagattga tgtattgact ttatttccaa gaatgtttga aggtcctatg
    11941 ggggaatcca tcatcggtaa ggctgtagat aaaggattat tagatatcaa tatctctaac
    12001 ttccgcgatt attcagataa caaacatcag acagtagatg attatccata cggcggtgga
    12061 gcaggtatgc tgctcaaagt ccagcccatc tatgataaca tccaagccat tgaaaaagct
    12121 gcaccggatg tcaaaaaaag agtaattctt cttgacccag ctggtaaacg attcgatcag
    12181 aaaatggcag aagaattttc acaagaagaa cacttagtat tcatttgtgg tcattatgag
    12241 ggatatgatg aacgtatccg cacattagtg acagatgaag tttccttagg agattatgtc
    12301 cttactgggg gagaattagg tgcgatggtc atgatcgatg caacagttcg gcttttgcca
    12361 gatgtgttag ggaatcagac ttcagctcaa acggattctt attcaaccgg tttattggaa
    12421 catccccaat atactcgccc ggcagaatat aaagggatga aagtaccaga agtattgaca
    12481 aatggaaatc ataaattgat tgaagaatgg caattaaaag aatcactaag aaggacctat
    12541 caacgtcggc ctgatctttt ggaaacactt gaatggacac ctcaaatgac gaaattttta
    12601 gaagaaatca agaaagaaga acaagaaaaa acagcagatg aagccaagtg actttttcta
    12661 aaatcttctt tacagtaggt tgataatatg ttagtatttt atggtgagtg caaaagcact
    12721 taactattac gatattccgc tgtgagaaaa gctcataaga atatttggaa taaggagaag
    12781 aaaaccatga atccattaat cgaagaatta acaaaagaac aattacgttc tgacatccca
    12841 gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa
    12901 cgtatccagt tatttgaagg tgttgttatc aaacgccgcg gtgctggaat cagcgaaact
    12961 tatacagtac gtaaagtttc taacggagtg ggcgttgaac gtacattccc attgcataca
    13021 ccacgtgttg caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac
    13081 tacctacgtg cattacacgg aaaagctgct cgtatcaaag aaatcagaag ataaacacgg
    13141 aaaacttcct tggaaatctg aaatttgtga aaagtccttg tatatcaagg gctttttctt
    13201 ttttctctat cacagtttta agccattttt ggctgttttg gaccattttt caatcatatt
    13261 cccttaaaaa ttgtcctaga ttgtccgaca ttgactatca gtgtcgtcaa aatagggcaa
    13321 aatttagtag tcagcttgac gacaaatata atacgaaaaa taccaaaaca tttaccgaat
    13381 ttagcaactt ttgaacccaa gtttctcaaa gggtcagcta ttagcaaaaa agacaaaaca
    13441 tgtacttttc tactaaaaag ttgatgtgat aggcttctat ctaaataact attaggagtt
    13501 tttatcgtga agcaaaatga tggacgtatc atttggaaaa atcaaagtga gttaaaactg
    13561 attttaacaa ttaatgaatt catcgaaaaa cacggaatta catctagccg tcaataccag
    13621 aaaaagctat cagaaaatcc aaattctgca ccaagtatgt ggttcattaa caaaaaatat
    13681 ggatcatggg agaatttatt gattagtata ggacgagaaa atactgggta tggaaaatgg
    13741 gctagaatgt cagaacaaga attattggaa atagtagaag cctttattaa gtgcgagaag
    13801 ataacctctc aacgaatgta cgaacaaaaa tcagtaggga agaatattcc ttcacttagt
    13861 accattaaaa agatgttagg agatatacgt ccacttttta aagaaaaaaa tgatggatct
    13921 cgatttactg atttcgaact gctgttagaa ctaaaaaatg aaataatccg attaaaacta
    13981 caggatgatt tatcaatgac aaagtttcga aaactagttc aatccccaaa actaccatca
    14041 gttgatacaa ttatgaaaag aacgaacaaa aattgggagg aattgatggc agagattgga
    14101 tttgattatc gaagaatcaa aatttataag cagagaaaca acctatccaa gacaaagaaa
    14161 actaaatagt atcactctaa atcagacgtt caaaattaaa atttggacgt cttttttatt
    14221 acccaaaaac gaaaggagat ttattttatg aaaaagccaa gatttaagaa ttggcgtttg
    14281 ctcgcaacac tgagtctatt atgccaaaca attggtggat cgcttggccc aacgattgcc
    14341 tttgctgatg aaattactca tccacaaacg gtaactgtgg aattggattt ggctcaccag
    14401 tatgctgtcg aaggtacatt tagcgatggt cgtcccatgt cagaagttac cgttccgcac
    14461 tatgcagttt acaatggtgt gaagcaagat attttctgta ttgagccagg ggtgccgatt
    14521 tacaatgagt tcactcctgg ttatgagaaa aatccattgc ctgatatgtc ggagaaagcg
    14581 aaattagttt ccgttttatg gaaggaagct ggtacggatg tggatacaca tattgtggcc
    14641 cagaagatga tttggcaaga agttaatggc tacacgcttc actcaattaa acgttctgat
    14701 ggtagtgcag taaatatcgc agccattgag tcaaaaatca atcaagccat tgcggattat
    14761 caaaagaaac caagcttcca taactccaca gtgaaaaccg tgttaggtca atcgactact
    14821 gtgacagata cgaacggctt gaatttatcg gagtttgatg aagttgtcga gaatatcgca
    14881 aatattgatt atcgtgtgaa cggcaatcaa ctagtgatta ctccaaatgc caattcaaaa
    14941 gagagtggtg aattaaccct aaagaagtca gctaatacag gaacaccggt agcttataaa
    15001 atggctggtc aacaaacttt gatggcgggg gcaattgata agcccaatac ctacacaatc
    15061 aaaattgatg tggaaactga gggttctttg aagattaaaa aagtcgataa agaaacagga
    15121 aatgttgtac cgggaaccgt tttccattta gactttggaa aaacattacc tgcaaaagac
    15181 gtgaccaccg ataaagaagg cattgcgaca ttggatgaga ttcctcatgg cgcgaaagta
    15241 accattactg aaaaatcagt gccagctcct tatacgattg acactacacc aatcaccgca
    15301 accattaaat ctggtgagac tatttctgtg acttcaaaaa atactcgcga aaaaggtcaa
    15361 atcattttga ataaaatggg ggtggaaaca ggaactgatc tttggaacga caaatactca
    15421 ttagccggaa atacgtttgc cattcgcaaa gacagtccaa ccggtgaaat tgtccaagaa
    15481 atgaccacgg atgaaaaagg tcatgcggaa acaccaaaag agattgccaa tgcgttggaa
    15541 ttgggaacct attatgtgac cgaaaccaaa gctagtaatg gctttgtgaa tacctttaaa
    15601 ccagtaaaag tcgaattgaa ttatgtcaat caaaccgtgg ctcttgttac cagtaacgta
    15661 aaagggcaaa accaagaaat tactggtgaa accactttga caaaagaaga caaagatacc
    15721 ggtcatgaaa ctcaagggaa agctgagttt aaaagagctg aatacacctt gtttactgtt
    15781 aaggatggaa aagcggtcaa atggaccgag aactttaaac cagaattgtt gaagggaacg
    15841 aaagcttctg atgaaaccgt aactttgact ttagatgaaa agaatcaagt tgccgttaaa
    15901 cacttagcga ttaatgagta ttactggcaa gaaaccaaag cgcctgaagg atataccttg
    15961 gatgaaacaa agtatcctgt atccatcaaa aaggtggatg atagcgaaaa aaatgctgtg
    16021 attactcgag atgttgcggc aaaagaacaa gtgattcgct ttggctttga tttctttaaa
    16081 tttgctggat cagctgctgg gactgccgaa actggcttca atgatctgac ctttaaagta
    16141 tcgccattgg aaggaaccag tgagattaca ggtgccgaag atgaagcaat tacagcttat
    16201 aacgagcaac taggttttga tggctatggc aagtttgaaa atcttcctta tggggattat
    16261 ttacttgaag aagtagaggc tccagaagga tttcaaaaaa ttacgccatt agaaatccgt
    16321 tctactttta aagaaaataa agcggattac actaagagtg aatacatttt tacgattacc
    16381 gaaaaaggac aaaaacaacc aattaaaacg gtgaccgttc cttacgagaa actgacgaat
    16441 aaagcatttt ctgttagctt gaatcgtttg atgctctatg atttacctga agaagaagat
    16501 agcttaactt ctcttgggac ttggaaagac ggaggcaaag aattgacaga ccttgattct
    16561 accgagctag ttgataaatt gagctataac ttgcatgaaa tcaaagaaga ctggtatgtg
    16621 gtagcgcaag ccattgatgt ggaagcgaca aaagctgccc aagaaaaaga tgaaaaagcc
    16681 aaaccagtgg taattgccga aacaaccgca acgttggcga ataaagagaa aacaggaact
    16741 tggaaaattt tgcataaatt aaccgcagaa caagttttgg ataaaaccat cgtcttattc
    16801 aactatgtgt atgaaaacaa gaaaccctat gaagcaggtg atgatccagt agcgaaagat
    16861 gctagcttga acaatcaagc ccaaactgtc aattgtacgg tggaacgcca tgtttccatc
    16921 caaacaaaag cccacctaga agatggttcg caaaccttta ctcatgggga tgtggtggat
    16981 atgttcgatg atgtgtcgat tactcatgat gtactggatg gctcaaaaga agcttttgaa
    17041 acaattctgt atgcattact tccggataat acgaccaaag aaatttggaa atctggcaaa
    17101 attgattatg aagtgaatga caaagaattc actaaaaccg tacttgcgga aaaagtggac
    17161 acaagtaaat atccagaagg tacaagtttt accttcaaag aaattaacta tggcaaagac
    17221 ggaaacatca atgggaaaca caatgaggat ctaaaagaaa aaactcaaac tttaacacct
    17281 aaaaaagttc cgacaacacc aagtgcacca gaacaaccgg aaataccaac tgttccaagt
    17341 gactctcaag aatctagtcc tacagtaaag acattcccac aaacagggga gaaaaattct
    17401 aatgttctgc tgtttattgg gtttaccttg atctttgcga ctgctgggta ttatttctgg
    17461 aatcgccgaa actaaggtga tgtgatgaaa caactaaata ggaaaaacca acgtgcgcga
    17521 ccgcctccta aaaaaagtgg tcgcgctatt tttgtatcca aaactaaaaa tgaaaagagg
    17581 aaaagaagat ggaattgaaa tttgttgtgc cagatatggc cgaaaccttt ggaaagataa
    17641 ggtatgccgg cgaaggcgaa gttttaactg aaggatatgg gcggaatact acagtaattg
    17701 gtcgtagtta tcatttgtat tccagtaagc aacgagccga tgatattgaa gtggtggtag
    17761 ctgcggaagc cggcgaaaag gattttgatc aagatcaacc actaaaagcc gtgaatcccc
    17821 atttggttgc caaaggctat gagattgaga atcgtgggtt taccgactac gtgttgtatg
    17881 tcgatgattt agtgaaagca taggaggaaa agaagatgag attaacagaa ggtattgttg
    17941 tagattcagg actaacgttt gggaaattac gattttccgc attacgccga gaagtacgga
    18001 agcaaaatga ggatggaacg gttagtaacg aggtaaaaga acggacttat aatttgaaat
    18061 cctctgcgca aggtcgaatg atccaagtta gtatcccagc aaatgtaccc ttacgcgagt
    18121 ttgcctatga cgcagaggta gagttggtca atccaatcat ggatacggtc gccaattatg
    18181 ttttccgaga aggaactact gtcaattggt tcattaaggc cgatgattta gtcttgaaac
    18241 gacaaccaga tcagggaaat tctacaaacc aaaatgaagg aaagaaatag gtgaagcgta
    18301 tggaaattaa tgttgaacat attttagatt gtctcgatca atatggaaaa ggtgagctaa
    18361 cggaggaaca actcactaaa gcattaacct atgatgaaaa aatgtttctg attatgcatc
    18421 aagggttact ggaagtaggt aacgatgccg aagaagattt tgatgtactg aattggttag
    18481 gggaagaaga gtccttcttt atggttatcg aagtaaatga aagtctatgt cgagaagccg
    18541 aaaccgtatt ggaagaaatt ggtgtggaaa tgccggatgc cattgaagtt tttttaaagc
    18601 aactagtgga aacgaaacaa ctccctatag cagtaaatga ctagcataac tgcttgaaat
    18661 tggatcaatg aaatcctatg aagctttttt cgattggagg tggaaaccat aaaaatgtac
    18721 aaagggcatc gtatacgggc aggagatcag cacttggttt atcactttgt tcttggctgg
    18781 ctactcgctc tgttcatcgg ttggatgagc gtcttttatt ttcaagagct tagacaattt
    18841 gatatttcta agctatcact ttctactata gaaatcgttc gttctataaa agatttaatc
    18901 tatttgctag gaagtctcgt tctttcagga agcacgatgt tgctttatat acactttttt
    18961 caggatcatt ggcgcagttt gtggcatcga caaaagctag cccggatgat tctagaaaat
    19021 cattggtacg aagtaaaaca aactcagagt gaaggatttt tcaaagacct caatagtagt
    19081 cgaaccaaag agacaatcag ttacttcccc aaaatctatt accgtatgaa ggatggttta
    19141 ctttctattc gcgttcaaat ttcactggga aaatatcaag atcagctttt aaagctggaa
    19201 aagaagttag aaagtggtct atattgtgaa ctagtagaaa aggaactcaa agactcctat
    19261 gtggagtaca ctttactgta tgatataatt gccaatcgaa ttgggataga cgaagtagtg
    19321 gcagaaagtg gggctttgcg attgatgaaa aatcaagtat gggcttatga ttctttacct
    19381 catatgttaa tcgctggcgg cacaggtggt gggaagactt actttttact cactatcatc
    19441 gaggctttat taaaatctga tgccgagctt tttgttcttg atcccaaaaa tgcggactta
    19501 gccgatttag gtacggtaat gcctcatgtc tattcccaaa aggaggaaat ttctgcttgt
    19561 gtggaagatt tctatgagcg catgatgact cgtagcaagg caatgaaaga aatgcccaac
    19621 tacaaaacag gagagaatta tgcgtttctt ggacttccac caaacttttt aatctttgat
    19681 gaatacgtgg tttatatgga aatgttaacg acgaaggaaa gtgcggtgat tttgaataag
    19741 ctcaaacaaa tcgtgatgtt aggtcgtcaa tctggtttct ttctgattct agcttgtcaa
    19801 agaccggatg cgaaatattt aggagacggg attcgcgatc aatgtgcgac atgtttctaa
    19861 ctgaaaaggt taggcactta aaagaagaaa acataagttt ccttttaagg agaactgata
    19921 ttttgagcag tggaatgatg gggtaacgcc ctgaaacgct cccttaatac tccgactggc
    19981 tgtttaacat atagctgtta gatggtcaga agctcggtga agtcggctga aagttaccgt
    20041 aaatatgata aattcatata cgaaagttgt tcagaggtga atgatgtaac ctataggtcg
    20101 gaggtctata aaatactcac ggtgagaatg taagtaatac tactgacgaa cttgcgaatg
    20161 aaagaatcta tacacggact gtatagaaat gtacaggatt ctaccgtatc aacggaacag
    20221 aatgtatgtt gtggtttagt aagattattg gttatgaaca accatgcaat gttacaggca
    20281 ttggaaatat acaggttcaa agcaagcacc taaaagtata tataaagata aaaatatcga
    20341 aacatgggaa gctggcaatg tggaggaaac tatattccat tgaaacattt gtgacgaaga
    20401 ataataagca catttatgcc agtgaaagtg gtggcacagt accaatgagg atatagaaat
    20461 atatcggagg aatagccact agtcaaaaga aatcgttaat taaatgatgt atttcataga
    20521 atcgagtaag acaaaataaa cactacaacc taagtaaaca aaggtagtgg tgctatgaat
    20581 aaagtaaaaa caaataaaac acttcgtcat agtgaatact atggcatgac acaaatattt
    20641 gatgaactgt atgtaaaaag tgataaaaaa gaagttttta cagagctcat gacaattatt
    20701 atgagtgatg aaaatattcg actagcatac agaaatatca agaataacaa gggcagttat
    20761 acagcgagta ccgataagaa aagtattcga gacattaaaa cactgtcaga agataccttt
    20821 ctaactgagg ttaaagttct ttttcgctgt tacaaaccac gagcagttag acgtgtagaa
    20881 attcccaaac caaatggaaa aacacgccct ctgggcattc catcaatctg ggataggtta
    20941 atacaacagt gtattttaca agtcttagaa ccgatatgcg aagctaaatt tcacaattat
    21001 agttatggat ttcgaccaaa ccgttccaca catcatgcga ttgctacctg caatcgactg
    21061 gcaaataggg ggaagctaca ctttgtagta gatgttgata ttaaaggatt ctttgataat
    21121 gtcaatcact ctaaactttt gcgtcaaatg tggacgattg gaatccgtga taaatggcta
    21181 ttaggtatta ttcgatccat gctaaatgct cctatagtcc taccaaccgg taaagtcgaa
    21241 caccctaaaa aaggtgtacc tcaaggtggt gtcctatccc ctttactatc taatatcata
    21301 ttgaatgaat tagattggtg ggttgcttca caatggcaaa ataccacgat tgagacagaa
    21361 aagattatgg ataggcgaga taaaggcaaa ggcattgata agactaatca gtatcgggca
    21421 ttaagacgaa caaacttaaa agaaatatac attgttcggt atgctgacga cttcaaaatc
    21481 ttttgtagaa aacgaagtca tgcagataaa atatttcacg caacgaaaat gtggcttaaa
    21541 gaacggttat cacttgaaat tagcgaagaa aaatccaaaa ttgtcaatct caaaaaaggt
    21601 aaatcagaat atctaggatt tgaattaaag ctaatcaaga aaggtaaaaa atacgtagtc
    21661 gaaagtcata tgagcaaaaa atcaatgaca agagttaaaa tccaattaaa gaaacaatta
    21721 cgtaaagttg ccagaccgaa gaatcattgc gaacaagcaa aagaaattgg attatataat
    21781 tctatggtga ttggaatcca tcaatattat ggaattgcaa cctgtgtaaa tctcgattgc
    21841 agtaaaatag cctatacact aaaaccgttc atcaatcaca aactgccgac aaagaaacat
    21901 ggaaagatat taaatacatt tatcaaatcg aagtatggaa aaagtaaaga aatgcgatgg
    21961 ttgaataatg tgccaattgt accgctcaac tatgttcagt ttagacttgc tgtcccccta
    22021 tcagagggaa cttgcaagta cactgaaagt ggacgaagca agatacacag taaattaaaa
    22081 ttagacctag cccttttact tcacatgatg agaaatagta attatgaaag aagtattgaa
    22141 tttgtggata acagaatatc taaatattct gctcaaaagg gaaaatgtgc tatcacgggt
    22201 aaatttctcg aatacgaaga aatccattgc caccatatcg taccagtaaa acaacagggt
    22261 acagacaaat acagcaacct cattattatt cacaaaaatg ttcacgcatt agtccatgct
    22321 atcgaagaaa aaatcataca caaatacctg aatgtattaa atctaaccaa tgaacaaata
    22381 gaaaaactaa atgaattacg tgtcaaagct ggtaatagtg ttttaacagt ttaacttgaa
    22441 aaatatatca tacttaacat tcttttgatg gaacgccgta tgaagtgaaa gtttcatgta
    22501 cggtgtgaag cgggggaaaa tctggagatg acatcaaagg attacctatc gc
//
LOCUS       DACBZN010000038        22132 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.038, whole genome shotgun sequence.
ACCESSION   DACBZN010000038 DACBZN010000000
VERSION     DACBZN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.038"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..523
                     /locus_tag="IVD64_001424"
     CDS             <1..523
                     /locus_tag="IVD64_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP6148682.1"
                     /translation="GGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGIVA
                     NCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFGS
                     LIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTVL
                     LLPLFFQPFAGLF"
     gene            523..2109
                     /locus_tag="IVD64_001425"
     CDS             523..2109
                     /locus_tag="IVD64_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6148683.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2128..2349
                     /locus_tag="IVD64_001426"
     CDS             2128..2349
                     /locus_tag="IVD64_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP6148684.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2346..3107
                     /locus_tag="IVD64_001427"
     CDS             2346..3107
                     /locus_tag="IVD64_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP6148685.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3487..3996
                     /locus_tag="IVD64_001428"
     CDS             3487..3996
                     /locus_tag="IVD64_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6148686.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4071..4610)
                     /locus_tag="IVD64_001429"
     CDS             complement(4071..4610)
                     /locus_tag="IVD64_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP6148687.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="IVD64_001430"
     CDS             complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="IVD64_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP6148688.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5677..6474
                     /locus_tag="IVD64_001431"
     CDS             5677..6474
                     /locus_tag="IVD64_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP6148689.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            6502..7137
                     /locus_tag="IVD64_001432"
     CDS             6502..7137
                     /locus_tag="IVD64_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP6148690.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7157..7930
                     /locus_tag="IVD64_001433"
     CDS             7157..7930
                     /locus_tag="IVD64_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6148691.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8338..9135
                     /locus_tag="IVD64_001434"
     CDS             8338..9135
                     /locus_tag="IVD64_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP6148692.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9113..9526
                     /locus_tag="IVD64_001435"
     CDS             9113..9526
                     /locus_tag="IVD64_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148693.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9510..12155
                     /gene="mutS"
                     /locus_tag="IVD64_001436"
     CDS             9510..12155
                     /gene="mutS"
                     /locus_tag="IVD64_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6148694.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12173..14281
                     /gene="mutL"
                     /locus_tag="IVD64_001437"
     CDS             12173..14281
                     /gene="mutL"
                     /locus_tag="IVD64_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP6148695.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14303..14863
                     /locus_tag="IVD64_001438"
     CDS             14303..14863
                     /locus_tag="IVD64_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP6148696.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(14948..16936)
                     /gene="treP"
                     /locus_tag="IVD64_001439"
     CDS             complement(14948..16936)
                     /gene="treP"
                     /locus_tag="IVD64_001439"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP6148697.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17159..17872
                     /gene="treR"
                     /locus_tag="IVD64_001440"
     CDS             17159..17872
                     /gene="treR"
                     /locus_tag="IVD64_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP6148698.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            17949..18395
                     /gene="msrB"
                     /locus_tag="IVD64_001441"
     CDS             17949..18395
                     /gene="msrB"
                     /locus_tag="IVD64_001441"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP6148699.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18565..19167
                     /gene="ruvA"
                     /locus_tag="IVD64_001442"
     CDS             18565..19167
                     /gene="ruvA"
                     /locus_tag="IVD64_001442"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP6148700.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19180..20181
                     /gene="ruvB"
                     /locus_tag="IVD64_001443"
     CDS             19180..20181
                     /gene="ruvB"
                     /locus_tag="IVD64_001443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP6148701.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20210..20788
                     /locus_tag="IVD64_001444"
     CDS             20210..20788
                     /locus_tag="IVD64_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6148702.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20840..21322
                     /locus_tag="IVD64_001445"
     CDS             20840..21322
                     /locus_tag="IVD64_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP6148703.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21439..21813
                     /locus_tag="IVD64_001446"
     CDS             21439..21813
                     /locus_tag="IVD64_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148704.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7237 a         3991 c         4790 g         6114 t
ORIGIN      
        1 cgggggcaat tttttgacta tgctgattac tcctgcgaca gtggcacttg ccatccctct
       61 ttataaaaac ttccgtttac tgaaagagaa tttttatcct gtaatggctg cgattttagc
      121 aggaatagtc gcaaattgct tggttagtgt agggttcggt tacttcttct ctcttcataa
      181 agagatggtg atctcattac tacctaaatc tgtaacgacg gctatttctg tcgatttatc
      241 tcatacgatg ggcggggtga atgccgtgac acttgctatt gttgtctcta caggtatttt
      301 tggttcgctg atcgccactc atattttccg cctattcaag atcgaaagtc cagttgctcg
      361 cggtgtggcg ttaggaagta cttctcatgc tattggtaca gcaaaagcaa tcgagattgg
      421 tgaaatagaa ggaatcattt ccggacttgc gatatgcgtc aacggaatat tgaccgtttt
      481 attgctaccg ttattctttc agccttttgc tggcttgttt tgatgcgaaa accactattt
      541 tatgctacaa taaccaaaac gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct
      601 tccgagtatc ttaaacaatt tgataaagat ctatattata agattctcct cctagaaagt
      661 tttgaggatc aagcttggca taccgctgca caattagcgc aagttgttca gctggatgcc
      721 cgcagcgtgt cgaaatacct gaacgagctg tctaaaaact atcagcagtt tagcggtaag
      781 acacaccctt tgtttacaaa aaatcaccgt tctggttata acttttatga tacgctcgat
      841 tcaattgaac atgagcgttt tttgatctat ctcgtgcaaa gtacgctgaa attccagttg
      901 ctgcacgaca ttttttttga agaattccat acaatgtacc aatttgcgca aaagcattac
      961 attagtgaat ctactgctca tcgaaaaatc aatgaatgga aacagcaact gcaaacttac
     1021 ggaattcggc tgcaacgagg tacatatatc gctcaaggcg aagaagaaat cattcgactt
     1081 tacttgcata tgacattctg gcagttgttt cgagggaaaa tctggccatt tgaaacgatt
     1141 tctcaaatgg acgtaaagaa tatggcagaa catatcatgg ctttttttaa tgttcgtctg
     1201 aacgagatca aaaaaagacg attggaatat atgctaggtg cctttttttt aagaaaatca
     1261 cagaagcatt atgttgtatt gaatgagaaa aaaagacggc tgatttctga caatctgttg
     1321 ttccagcgat tttgccaggt catggagccg gtatttccta attattttca agtagaagac
     1381 gagttaggcg cattatttct tgtactgatg actcgtgaag aatattatag tgatcctaag
     1441 atacggaaga aaatttttga ttttcatcaa caagcaaaaa caccgccctt tacagccctt
     1501 tcagaagcaa aagccgctct ttctctttat caggaagagc aggggctgcc agcagaaaat
     1561 ctaaccttcg aggcagaaaa ctatttattc agcagtcatt ttttcgccta tctttttcct
     1621 aatgcgaaag aaacgattga tggcaatagt agtgatttta ttaatcattt agttattgaa
     1681 aacaaggaat tgaaacaatg gctcgtacat ttttttgaaa gtagacacaa acatcccaat
     1741 catctggcat ttaaaaatca tgcgtttttg atggggcggt atctgactgt gtttaaaaca
     1801 ttaggagcct ttactccaca gcttccaaag atcacgatcc ttttaatgac agatttccca
     1861 ttatttgaag agcaactgtt agaggaggga ttacggacat ttttccgtaa tgagtatcag
     1921 ctgatttttc tgccaacaga ttatcggggg agagaagttg atcttctgat ttcgacaagt
     1981 aaagtccacc gcaaaccatg ggcggatctt gactatttta ttgtgactga agaactaaaa
     2041 ttaattgatt acatccaact atctcaaaaa tttgaaatga ttcaaaaaca aaaactatta
     2101 aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc agaattgaaa
     2161 aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt tgttctcgta
     2221 tccgatgatg aatttgatgg ctgtccggtg acgccgtact tattgatcaa aacaagtgac
     2281 gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga ttgggcgatc
     2341 gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc acaggagctg
     2401 cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct tttgtatttg
     2461 gattagatca aaagttcggt aaaatggaag aaatttcttt gcgatttcct gaaacttttg
     2521 cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta tcgaaatgcc
     2581 aaaatcattt tggtgaagtt acgattttac tgaatacagc ggggatattg gatgcctaca
     2641 aacccttgat ggaaactgac gaatcgctgt gggatctgat ttatgagacg aatgtgaaaa
     2701 gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccaaaaa agcggaacga
     2761 tcatcaatat ggcttcgatt gctggcttga ttgctggtgg aggtgggatc gcttatacaa
     2821 gcgcgaaaca tgcgattgtc ggatttacca aacaactcgc tctggattac gcaggacagg
     2881 gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat gcggctgatt
     2941 ttgaagggaa aggtgagatg gcagagtggg tggcaaatga aacaccagtc aagcgatggg
     3001 cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa gcaagctaca
     3061 tacaaggaac gatcatccca atcgacggcg gatggttgtt aaaataacct tgtgtttttt
     3121 tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa aaaaagtcga
     3181 tgactgttcc cgacattcga tgccgaacag ccagttcaaa aaattctttt taataatcgt
     3241 taatattcga aactaagcgg cttttcacga cctcttgata taaacgatat tcaaaagctt
     3301 gcaactgcgg taacaagtcc agccaaatga aaaatataag gagctatttt tatttgacca
     3361 ttcttattac ttgttgcagg gcagcttttt cactaccttc tgcgacactt cagttggaat
     3421 tttcttccca tcctttggtc gcggtcatta ccaagataaa attatcttgc aaaggagaaa
     3481 tagaacatgg aaaaaaataa tacccgttca gccaaacgct ggacaacgac agatgtgact
     3541 cggatggcgc tagtcactgg tttatatgtg gcagtgacgc tggttttgtc tgtctttagc
     3601 tttggagtga ttcagattcg tctctcggag atgttcaact atctgtcctt gtataacaaa
     3661 agatacatcg tcgcggtaac agcgggagta gctttggcta atattgcttc tcctcttgga
     3721 ctgatagatg ttgcagtagg aagcgtcagc acgctgcttg tattgcttat caactataaa
     3781 atcactagtc gcatcaaaaa catgaaaatc aaaatggccg tgacagcatg cgtatttgct
     3841 ttttccatgt ttacagtagc tggtcagcta acgattttat atcaactacc ttttttcttg
     3901 aattggtggg tgattgcttt aggagaattg gcatcaatga ttgtcggtgg gataattatt
     3961 tattggatcg gtaaaaaagt ggatttgtca aaataaagat ctgtttaatt taaaataggg
     4021 cagcatggaa gagactgaat gttgagtctt cttccatgct gcttttttga ttaatttgta
     4081 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     4141 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     4201 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     4261 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     4321 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     4381 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     4441 ccaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     4501 gtttgccctg tcaaaggaac caaaccaaaa ggaatggtca tctgagaaag gacagaaata
     4561 atcgcggcaa attccgcagc aagtatcaat tctttcaatg ttcggttcat aacgccctct
     4621 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     4681 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     4741 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     4801 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     4861 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     4921 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     4981 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     5041 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     5101 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     5161 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     5221 gtattctgat tgtttgccac ggctattagg tccatgttga cctggtgcgt aagggcgacg
     5281 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     5341 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     5401 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     5461 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     5521 ctgcattatt ttacacaaat tacagtatat gatagatcat ccacgaatgt caagataatt
     5581 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gtatgtgcaa
     5641 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     5701 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     5761 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     5821 gcgatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     5881 atgttcagct ggggtctggt catgattcta tacatgaacg ccgaattagg aacatcgaat
     5941 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     6001 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttctaatt
     6061 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     6121 gtaaaactag caaaaccttc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     6181 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgtcggaaa agtgatcaca
     6241 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     6301 cctgccttta cgcttactta ctttgcttct cacggacaga tggatggaat gacagtcagc
     6361 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     6421 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     6481 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     6541 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     6601 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     6661 ttagcttttg ttcattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     6721 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     6781 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     6841 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     6901 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     6961 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     7021 aaagcaattg gttgggcatt acgcgattat agtaagaccg atgccgattg ggtaaaaaga
     7081 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     7141 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     7201 agattcaaac aaacaagctt tagatagcac aagagaagca ttggaaaaat tacgcaaagc
     7261 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     7321 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     7381 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     7441 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     7501 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     7561 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     7621 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     7681 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     7741 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     7801 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     7861 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     7921 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     7981 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagctttcg
     8041 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     8101 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     8161 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     8221 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     8281 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
     8341 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
     8401 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
     8461 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
     8521 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
     8581 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
     8641 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
     8701 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
     8761 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
     8821 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
     8881 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
     8941 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gactgcatta
     9001 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
     9061 ttggatgata cgacaggtca tgcaaaagaa atccagctga ttcagatcaa cgatgatcga
     9121 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
     9181 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
     9241 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
     9301 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaaa aagaagcaat
     9361 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
     9421 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
     9481 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
     9541 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
     9601 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
     9661 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
     9721 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
     9781 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
     9841 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
     9901 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
     9961 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    10021 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    10081 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    10141 ttttgacaag cgaactagtc gatccgctgg aaaaagaagc aaccggcaaa ctattgactt
    10201 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    10261 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    10321 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    10381 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    10441 aagcaagaca agaaatggtc gcttctttgc tagacagcta ttttgaacgt atcgatttgc
    10501 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    10561 atgtcaatgg gcgagacttg atccagctga aaacttcttt agaacaagtg ccaatgatca
    10621 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    10681 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    10741 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    10801 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    10861 gaatcaagaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaattacaa
    10921 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    10981 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    11041 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    11101 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    11161 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    11221 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    11281 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    11341 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    11401 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    11461 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    11521 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    11581 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    11641 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    11701 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    11761 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    11821 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    11881 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    11941 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    12001 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    12061 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    12121 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    12181 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    12241 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    12301 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    12361 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    12421 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    12481 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    12541 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    12601 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    12661 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    12721 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    12781 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    12841 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    12901 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    12961 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    13021 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    13081 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    13141 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    13201 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    13261 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    13321 cgtagaagaa ctaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    13381 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    13441 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    13501 aaagaaagaa tttcccattg tggataacat ggagaaaaca aatgaagaac aagcagataa
    13561 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    13621 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    13681 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    13741 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    13801 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    13861 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    13921 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    13981 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    14041 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    14101 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    14161 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    14221 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    14281 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    14341 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    14401 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    14461 aactgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    14521 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    14581 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    14641 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    14701 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    14761 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    14821 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    14881 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    14941 actctgacta agattttact tcagctttac cgactgcttc gcccgggcga acaagtcctg
    15001 attggtcaag agaaagtcct gcaacttttt cattattggt caaagcaaca ataatgtcag
    15061 ttttcttgcc agcttgttct atttctggca aatccatttc agcaatcagg gtctctggtg
    15121 tgacaaggta tccttctttc acgaatacat tgaaaggaac accctttagt tcaacagtat
    15181 ctaatcccat gtgaacaagt acttccagcc cctcattaga aagaatacca atggcatgtt
    15241 ttgtttcaaa gacactcgtc actttaccgt ttactggtgc atagactttg ccgtttgacg
    15301 gttcgacagc ataaccgtca cccatcattt tttgtgagaa aactgggtca tttacttcag
    15361 ttatttcctt gatggtccct gcagcgactg cgaataatgt ttcttttgtt gcaactgccg
    15421 taccagttgc tgaaacaggt tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt
    15481 gaagttgtac atcagcagca ttttcaggaa cagcaggatc aatcttattc aaaattccct
    15541 gacgtctaaa gatgatcgtc aagccaaatg gaatgatgac agcaatgatc attgcgatga
    15601 tgaaaggtac ccatgtttct gcttggatag ctagtattcc tggcaaaccg cctactccga
    15661 tcgcgttagc tcgaacaccc atcaagttag caaacatgcc agctaatcca gagccgacca
    15721 ttgcagcgac gaaaggatag acgtatttca aattgattcc gaacatagca ggttcagtta
    15781 cgcctaagta acaagaaatc attgctggaa tcgagatttg ttcttctttt ttattcccac
    15841 gatgtaagaa gatgatggct aagactgcag agccttgggc gatatttgat aaagcaatca
    15901 tcggccataa attcgttgat ccgaaatctg caatcagctg catatcgatg gcattggtca
    15961 tatggtggag accagtaatc actaaaggag catacaggaa tccgaatacg gcactaaaca
    16021 accagctgat agaagacgtc aatccagtat ttacgatatt tgagatccag cttccgactg
    16081 tccagccgat aggacctaaa ataacgtgtg cagctaaaac agttggtaac aatgaaaaca
    16141 atggaacaaa aatcatcgag atcgactgag ggatatattt gcggaagaag atttctagat
    16201 acgctaacat aaaccctgcc agcatggctg gaataacttg cgcttgataa ccaatcatct
    16261 ggacttgagc aaaaccaaaa tcccaaaatg gaatatctgc tgcggctgtg gaagctacac
    16321 tataagcatt taataattgc ggagaaacta acgtaattcc taaaacgatt cctaatattt
    16381 gtgtagtacc catctttttt gcaatactcc aagtgatacc tactggaaga aaatggaaaa
    16441 ttgcttcacc cggtaaccac aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa
    16501 cgattgttcc accaagactt tcaaactgga taccttcaag tacatttctg aatccaagga
    16561 tcaatccacc gacaatgatt gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa
    16621 tagcacgttg aacgggatga aggttttgtt ttgcagctgc ttttccttgt tctttggaaa
    16681 ccccttcaac tccagatact gcaacgaagt cgttataaaa agtagaaaca tcgtttccta
    16741 tgatgacttg gaactgtcct gcattcgtga acatcccttt tacgctaggt atatcttcga
    16801 tcgctttttc gtccgctttt cctggatcat tcaggacaaa acgcatacga gtcgcacaat
    16861 gactaactgc agcaatattt tcttttccgc cgatatcatg taacagcttc tcggcatctg
    16921 cttgatattt tgccatatca ataaacccct ttctcttaac ttgtctatgc aagttgtttc
    16981 ttcatttact atactttttt tcggagagaa ttgcaagcgt tttattaaaa agtacgagag
    17041 aaaatgatat cgttttaaaa agcttaaaat aagcttttct taaaaatagg aacattgaaa
    17101 tacaaaaaga gctgtagtat agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat
    17161 gaataagttc aatgaaattt ttttagattt ggaacagaaa attttagcga aagaatatcc
    17221 tccgcattcg cttttaccca gtgagaacca attgatcaaa atttatggtg tttcacgaga
    17281 aacaattaga aaagcgctga atcttttgac tacggaaggt tatattcaaa aaaaacaagg
    17341 aaaaggttcg atcgtactgg atcggaatcg cttcgacttt cctatttctg gattgactag
    17401 ctataaagag ctacaggaga cccaacgtat tccaagtgaa acgatcatcc atacgttaga
    17461 agaaacagaa gtgacaaaag cattgaacga gatcactggc tgggagatcg gtgcgcctgt
    17521 ttggcatttg atacgcgaaa gaaaaatcaa tggcgaagta gttatattag acaccgatta
    17581 tctattaaag gaaatcgttc ctattttaac tccagcacaa gcacaaggat cgatctatga
    17641 atattttgaa aatgagcttt ctttgacgat tgcttacgcg caaaaggaga tcacagtaga
    17701 agaagtaacg gaaatcatga aaacagcgat ggatcttcac gatgacactt atgcagtagt
    17761 agtcagaagc ctcgttcatc tggaagatac ccgctgtttc gagtattctg agtcggttca
    17821 ccgattagat aaattccgtt tcgttgagtt tgcccgacgc cgaaaagtgt aaatagttca
    17881 aaagaaagca aattgttggc atctgtccac tttggcgcta tgataggatc acaaaggggg
    17941 catttttaat ggataaacca ataagaccag aaaaagaaga actgaaagaa aagttatcac
    18001 ccatggctta tgcagtcaca caagaaaatg caacagaacg accatttagc gggaaatatg
    18061 atgattttta cgaaaagggg atctatgtag atatcgtcag cggtgaaccg ttattcagct
    18121 ctgcagaaaa atatgatgca ggttgtggct ggccatcatt cagcaaacca ataacgaaaa
    18181 gacaagtgcg cgaaaaagcc gatttttctc atggcatgca tcgaatagaa gttcgaagca
    18241 aagaagcaga ttctcatctc ggacatgtgt ttaccgatgg accagtagat caaggtggtc
    18301 tacgttactg catcaattct gcctcactga aattcattcc atatgaaaag atggatgaat
    18361 taggttatgg ggaattcaaa tcacttgtag aataatggaa atacacaaag agcggtacaa
    18421 tacggggatt tactcgatcg tccgctcttt ttttattcaa gcgattgcta tttgaaagaa
    18481 acgctcccta gactcctttt ttcacaaggt gaaatgtgct acaataaagg gaacgtatgt
    18541 gtgcaaaaga aagaggacta gaatatgtat gaatatctga caggtgtggt tacctttatc
    18601 aacccctatt acatagtagt agaaaccaat ggtatcggct accagatcgc actaggaaat
    18661 ccttaccgct acagtagcaa actaaatcaa aaaataaaaa tttatgtcca tcaggtaata
    18721 cgtgaagatg cgcatcttct gtatggattt gattcgctag aagaaaagca actgtttttg
    18781 cgattagtaa gtgtttcagg aatcggtccg aaaagtgccc ttgcaatcat ggcaagcgaa
    18841 gaccactccg gtttgattca agcagtagaa tccagtgatg tcacttattt gacgaaattt
    18901 ccaggagtag ggaaaaagac agcacaacaa atgatcttgg atctaaaagg aaaattcggt
    18961 gagctgagta tcgatacacc ttttagtcta tttgatgaag caaacacaaa agacgctaca
    19021 gctctatctg aagcaatgga agctctctcg gctcttggtt acagtgatcg agaaatcaaa
    19081 cgtgtagaaa aacaattaaa agaagtagat aatcagacaa cggatgagta tctacgccaa
    19141 gcactcaagc taatgatgaa aaaataagga ggagaccaaa tgacagatga atacttgtta
    19201 tcaccagaat ctggcgaaaa tgaactgagt ttggaaaaat ctcttcgacc acagctgctt
    19261 tctcaatata tcggacagag aaaagtcaaa caagaacttc agatttacat cgaagctgcc
    19321 agaaatcggg aagaaccttt agatcatgtt ctgttgtacg ggcctcctgg attaggaaaa
    19381 acgacgatgg ccatggtcat tgctaatgag atgaccgtaa atattcgtac gacgagtggc
    19441 cctgctatcg aacgtccggg tgacttagta gcaatcttga atgaactgga agcaggggat
    19501 gtcctgttta tcgatgagat ccatcgatta cctagagtgg tagaagagat gctttattca
    19561 gctatggaag atttctatgt agatatcatg gtaggacaag ggccaaccgc acatccggtt
    19621 catttccctc ttcctccctt tactttgatt ggcgcgacga cacgggcagg tatgctttct
    19681 gcacctttac gagatcgatt tggcatcatt tctcatatgg aatattatga cgaacaggat
    19741 ctgcaggaga tcgttttacg ttcttcggat atttttcaaa cagagatcat tgaagaaggg
    19801 gcattggaaa ttgctcgccg atcacgagga acgcctagaa tcgctaatcg cttgctcaaa
    19861 cgggttcgtg attttgcgca agtccaagga gacgggaaaa tcgatcgtcc tatagcggat
    19921 caggcactta ctttgctaca ggtcgatcag gctggattgg attatgttga ccagaaattg
    19981 ttgcgaacaa tgatcgagtt atatggcggc ggaccagtag ggttaagtac gctttcagta
    20041 aacataggag aagagcgtga gacggtagaa gatatgtatg aaccgtatct gatccaaaaa
    20101 ggatttttaa agcgtacacc tagagggcga attgctactg cttatgccta tgaacatttt
    20161 ggttacgact atcatatatg atttttgctt ttagattgga ggaaaatgaa tgagtgatac
    20221 aacacgtacg aaacaattga tcaaaaacgt attgattgat ttgtgccaaa cgcaatcttt
    20281 tcgaaaaatc agcgtccaaa aaatcgcaaa cgaagcaaaa atcaatcgac aaacatttta
    20341 ttatcatttt acagataaat acgatttgct tcgttgggtc tattgtgagg atgcattgca
    20401 ttatttagat ccacaatgtc tttcacttga taattgggaa gagcaagcat tgaaaatgct
    20461 gaaagcaatt gctgagaaga aagaatttta tgcaaatact gtgctttctg acccagaaat
    20521 tttacaaaaa gagttttctc aactgatcca aaaatcattt atcaagattt ttgaacaaat
    20581 ggatgaagaa agacaacttt ctgcaagaga caaactattt tatgctcgtt ttttttctta
    20641 cggttgcagt ggtgtattag tcaattggat cacgaatgaa tatgaagaaa caccgatgga
    20701 aatcgctatg cagcttttcc gtttagccaa ggatacggaa ttatattctt atcgtcttta
    20761 tgcaaaagaa gagttggaag aacagtaata gaaaaatttt ttgttggaaa agcaataatc
    20821 gctaaaagga gatggggaaa tgacaaaaat tttatttgtt tgtttgggaa atatttgccg
    20881 ttcaccgatg gcagaaggct tgctgagaaa acgaatagca ttagaaggaa gggaagcaga
    20941 attttttgtg gattctgctg ctacaagcac gtatgaagtt ggaaagacac cgcaccctgg
    21001 aacgaaaaaa atattaaacc aagaaaaagt agatatgacg aatatggttg ctcggcaaat
    21061 cacaccccac gattttgaaa cttttgattg gatcattggc atggatcaag aaaatgtaga
    21121 agaactgaaa cgaagagcac cacgctcagc gcaggggaaa atccatttgt ttttaagtag
    21181 cattcctgga aaagaaaaag aaaatgttcc cgacccatac tataccaata actttgatga
    21241 gacatatcac ttgatcacag aaggcctgaa tcattggtgg aatatttgga ttgatgaaaa
    21301 taaaataaca aacaatttgt aaagttaacg ctttcttttt aaggcgatgt tagagcattt
    21361 ccttgttttt ttgtaaaatg ttaggtaaaa taaaattaat taagtaacac tttcttagct
    21421 aaaggaggag tgcattcgat gaacgaagag acagtatcgc caatgaagaa gcttacgaag
    21481 ttagttactc atacttcgta tcaacttttt gattcttttg taaatgacac tgaagaacat
    21541 gctaagaatg tacgattgca aggaaagttt attcttgagc tgcaaaaagc tgctactgaa
    21601 catagtctag ttatcttaca agtaagagca aatcgtttga gtagcaagta tgaaacgatc
    21661 tcaggctggg tggcgacaaa aacggtcgaa aaagaccgag tgatgatccg cctacaaaac
    21721 gaagagcagc agatccggat ggttcctgta gaacaaattg aaaaaataac aatcctttca
    21781 ccagcagggg aacaggaacg gttatcaaaa taatttatac cagatagaaa agcacgtatg
    21841 agcttgtacc atcaatcatt actaatcaaa aataaccgaa tgctttaagc tgatatcagt
    21901 aaaacggaac gttttaggat gagtatcata gcatgacagc attcggttga ttagttgaat
    21961 cgattggatg gtacaagttt ttttgataaa aaaaggtcct gctagccggg gagctagcag
    22021 gataaggagt taaaaatgaa aaagtgttgt tagggttgtt tgttggtatg cttatataat
    22081 agtcattagt tgtgaatatt ttgtgacgaa agttttcgaa agttgttaag tt
//
LOCUS       DACBZN010000039        21442 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.039, whole genome shotgun sequence.
ACCESSION   DACBZN010000039 DACBZN010000000
VERSION     DACBZN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.039"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..941
                     /locus_tag="IVD64_001447"
     CDS             <1..941
                     /locus_tag="IVD64_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HAP6148705.1"
                     /translation="AVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQAPT
                     GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDAN
                     GTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVEL
                     TKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAP
                     GAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGN
                     KHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1365..1535)
                     /locus_tag="IVD64_001448"
     CDS             complement(1365..1535)
                     /locus_tag="IVD64_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6148706.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1729..1908)
                     /locus_tag="IVD64_001449"
     CDS             complement(1729..1908)
                     /locus_tag="IVD64_001449"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148707.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2295..3407
                     /locus_tag="IVD64_001450"
     CDS             2295..3407
                     /locus_tag="IVD64_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6148708.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3485..3778)
                     /locus_tag="IVD64_001451"
     CDS             complement(3485..3778)
                     /locus_tag="IVD64_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP6148709.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3946..4260
                     /locus_tag="IVD64_001452"
     CDS             3946..4260
                     /locus_tag="IVD64_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP6148710.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4281..6260
                     /locus_tag="IVD64_001453"
     CDS             4281..6260
                     /locus_tag="IVD64_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6148711.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6273..6599
                     /locus_tag="IVD64_001454"
     CDS             6273..6599
                     /locus_tag="IVD64_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP6148712.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6615..7133
                     /locus_tag="IVD64_001455"
     CDS             6615..7133
                     /locus_tag="IVD64_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148713.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7133..8485
                     /gene="celB"
                     /locus_tag="IVD64_001456"
     CDS             7133..8485
                     /gene="celB"
                     /locus_tag="IVD64_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6148714.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8569..10074)
                     /locus_tag="IVD64_001457"
     CDS             complement(8569..10074)
                     /locus_tag="IVD64_001457"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP6148715.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10166..10756)
                     /locus_tag="IVD64_001458"
     CDS             complement(10166..10756)
                     /locus_tag="IVD64_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6148716.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10935..12581
                     /locus_tag="IVD64_001459"
     CDS             10935..12581
                     /locus_tag="IVD64_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6148717.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12630..13349
                     /locus_tag="IVD64_001460"
     CDS             12630..13349
                     /locus_tag="IVD64_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6148718.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13828..14775
                     /locus_tag="IVD64_001461"
     CDS             13828..14775
                     /locus_tag="IVD64_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148719.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14787..15707
                     /locus_tag="IVD64_001462"
     CDS             14787..15707
                     /locus_tag="IVD64_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6148720.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15748..17205
                     /locus_tag="IVD64_001463"
     CDS             15748..17205
                     /locus_tag="IVD64_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6148721.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17550..18164
                     /locus_tag="IVD64_001464"
     CDS             17550..18164
                     /locus_tag="IVD64_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6148722.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18157..19881
                     /locus_tag="IVD64_001465"
     CDS             18157..19881
                     /locus_tag="IVD64_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6148723.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19878..21329
                     /locus_tag="IVD64_001466"
     CDS             19878..21329
                     /locus_tag="IVD64_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6148724.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6916 a         3635 c         4352 g         6539 t
ORIGIN      
        1 gagctgtttt tgagttacag aaccgagaag gagaaacgct acaaaccggt ctaactacag
       61 gagaagatgg aaaactggca attgacggtt tagcaccagg agcatatcag ctggtagaaa
      121 ctcaagcgcc aacggggtat gaattagatg cgacaccgat cgagtttgag atcgaaagaa
      181 gtcagacagc ggtggtagaa ttgacgaaag caaaccgact tacaccaggc ggtgtcgtgt
      241 tgacaaagat cgatgaccaa agcggggaag tcctgcaagg agctgttttt gagttacaag
      301 atgccaatgg aaccgttctg cagtcaggtc taaccacagg agcagatgga aaactggcga
      361 ttgacggctt agcaccagga gtgtatcagc tggtagaaac ccaagcgcca acggggtatg
      421 aattggatgc gacaccaatc gagtttgaga tcgaaagaag tcagacagcg gtggtagaat
      481 tgacgaaaga aaatcgactt acaccaggcg gtgtcgtgtt gacaaagatc gatgaccaaa
      541 gcggagaagt cctgcaagga gctgtttttg agttacaaga tgccaatgga accgttctgc
      601 agtcaggtct aaccacagga gcagatggaa aactggcgat agatggctta gcaccaggag
      661 catatcagct ggtagaaact caagcgccaa cggggtatga attggatgcg acaccagtaa
      721 ctttcaagat cgaaaaagaa caagaagtgg cgatcttttt aacaaaagag aatagaaaag
      781 ttgctgatag ctcagatata agaggaaaca atccaacaag cagtggaaat aagcatcttc
      841 caaaaactgg agaaaccatc attagccagt ttatattgtc aatattgggt gtgctacttg
      901 ttttcatcag tataaaattt agaaagaaac gaaatatatg agctaaaata aaaaattgaa
      961 taagcatagt ttttaaaatc aactagtacc catcaattga atggaaacta ccgattttag
     1021 aaggggtata caaacaaata ttagtatcgg agtagagaag gttgaaccca accagtaaat
     1081 aagtaaagac aattttgtgc tgcgttgaga agtgaaaaat aaacctttgt agtaatacta
     1141 gttaataaaa aatatacaaa aacactgcga aaatcattct tgagtttcgc agtgtttttt
     1201 gcctaatgac tcaagtttat tgggtaatac tttgattttc tttctacaaa taatcagcga
     1261 tcagttgtaa ttttttctct agttttaatg gctctttgtc aataaggaga aaaacaagtc
     1321 gggtcttttg tctcggaaag cttcaaccaa ctcgtgaata aaggttacgc ctccttgaaa
     1381 ggcgtggaaa aaccaagtaa tcgatcaatc accaatttcg catttggtag aacatgtttg
     1441 gtgagctgaa agtaggtggc gttcatgtct gtaactaaga attctatttc tttaggatta
     1501 gtgcagctaa ggaaatagtt tgttaatcga ggaatttttt gtgtaggtaa attttctcga
     1561 ttaatttttc tgtctcgcca tccgcggaaa taaaactcag tttatcctca acggaaacgt
     1621 gagaacgaaa ttcacaaccc tcaacactca agacagaatt cttttggaag actttggtaa
     1681 atagcttctc aactctttca atgtgcgaat aacggttgtc aaagatacct aacaactttt
     1741 cgaaataaaa aataacgaca ctttttcagt cagtaaagaa gtgattttat gtctgacatg
     1801 atttgaaatc gagtgccttg gacggataaa ataactttga gcgttccaat ggcttcgaca
     1861 gtttttacag gtatagcgct gcttttttag gcgtataacc attggcatat gattatatta
     1921 ttcaaagtgg acaatggttt ccttcttctt gtttttcacc accattaatt tcctatttcc
     1981 atctaccaca gcagaaccac aatttctaca gacacatgga gcagggaaga gaactgcagc
     2041 aacgatcaat gtttttttct tctgtaaggt ttcgtaagag acctctgtaa tcatcaaatt
     2101 tttgtctatt gatctcagaa tttttttgat agaaccattc ataaagcata tcgttctctc
     2161 agttgtttat tttgtggtga tttaatcata caagaaaacg atatgttttt ttataaaaac
     2221 atgaatagga aaaatagttg acaagaaaat gacaacgcca tatgatgttc catagtattt
     2281 acaacctgtc atagatgact gtaaaaaata aaagtaaaaa gaaaggacga caacaggtgg
     2341 atttttatca gtctctacaa ctagatccgt ttattttaaa acaaaaaata cgagaagcag
     2401 cttctaaaaa agaaaagtgc atgtatattt gttcattgtt tttacgatca ttattcatcg
     2461 tattgtttgc catttgtttt atcattataa ttacaacatt atttgaatcg aagcataaac
     2521 catacgctgt ggttttattc tgtatgttga tgagtattcg ttttgttgac tttggttata
     2581 agatttccca ttcgatcata ggtttagcaa tcgtcatgtt ctcactatta gtagcaccgt
     2641 atgtccagct gatcaaatgg tcagttatgg gcatgttgat tcattttatt ttactaagca
     2701 gcatattaat ggcaaccgct agtgatccca aaatggggaa tgcttctttg tacggctttt
     2761 catatctctt tattgtttat tcgttaccta aagattcgtt gaacaagaat ttctttacac
     2821 aaacaagtag cttattattt ttgttttttt gttggttttc agttctcctt tacagaaaac
     2881 atcgagaaaa gaacaaagac aaaagcttgt ttaaagagat ttttttgaaa ggcatgtatt
     2941 cgcaggaaaa aatttggatg ctcatctatg catttggcat tagtcttttg atcgttgcag
     3001 gagagtatgt cccttttcag cgattgatgt gggcagggtt tgcattttct tctattgtat
     3061 ctagctatgg attgatgtcg attggtttca aagaacgagc agtagaccga atcattggct
     3121 cattgattgg atgtgtttta tttattggta tcagccaatt tattcctttt aactgggtcg
     3181 gtatattagg tggtctagct ttaggtgttt gttctaccta tagatataaa acagttttta
     3241 attcttttgg tgctttggca atcactgcca gtttattcgg ggttccagga gcagtaacga
     3301 tccgcatttt tgagaatatc cttggggtat gtttaggaat catgtatatt ggggtgactg
     3361 aaatacttat ccgaaaaata agagaaaaac acggtttgaa tcattagatg agatcgaata
     3421 aaaaataagg aaaaagtgaa gatacacatg gatgataaat tgctatcatt ccatgtgtat
     3481 ctttttacgg taaatcagtg aaacgttcga ctttcatttt taatttatac tttttacgga
     3541 gttcgtttat ttgagccatc atttcggatt tgtgatgctt ttcgattgct tcttcatctt
     3601 tccaacggtc gatcagtaaa agagattctg gatcttctag tggaaagtaa tagtcatatt
     3661 tctcgtttcc ttcttctgca cgaatcgctt tgacaatgtt tctttcgacc atttcttctg
     3721 caaattttct ggcttgtcca tttgtacttg tatacaaaat attcactgtg atactcacta
     3781 aatcccctcc tgaaaaaatt ctatcactag tatatcacgg tgccagaaat tattattcaa
     3841 gatatgatac acattttttc cgcaatgcta cggaaatata tattttgaat agaaaacgct
     3901 tacaagatat gcttggttta taaaaataaa aggagtgatt gatttatgaa aaaagcattg
     3961 attatttgtg cggcaggaat gtcatcctcg atgatggcct caaagacaac ggatttcttt
     4021 aagcaaaaaa gagaagaggt actcgtagat gcagtttcag ctacagaagg cgataatatg
     4081 attaagaata gtgattttga tttatttctg atcagcccgc aaacgacgat gtttttggat
     4141 aaattcaaaa aattaggaga atctgttgga aagcctgttg ttagtatccc tttccaagcg
     4201 tatgtcccaa tccctacagg catccagaag ttagcagagg tgatcgaaga gaacatatga
     4261 tggaatagga gtggatgata atgaaacaga aggaaaaatt gctgttgaga tatttgattg
     4321 atcacaagaa ggagtatgtc accagtcaac gtttagcttc tgaattgtta ttatctgatc
     4381 ggacaatccg taattacttg cagcggatca aagagttagt agaaaaaaac ggaggtaaga
     4441 tcattgctaa accaggttat ggctaccaac tgcatatttt acaaagactg acatttgact
     4501 tgtttttgag tcggcaagaa atagtacctg gatatgctag agaaccgcaa ggattttatg
     4561 agtccgaaga tcgacaaaag tatatattga accaattgct tttggaagat gccattcttt
     4621 ttatggatga tttggcagaa gagctttata tcagtcgttc cagtttgaca aaggacatgc
     4681 aagaaatcaa ggagcgattg attccatatt cactaaaaat cgtttctaag catggtcaag
     4741 ggacatggat cgaaggggaa gaaagaaatc gacggcattt tatcatggat actttttttg
     4801 gaaaccatta tggaaattca ttgaaagaat atttagggca aagccgtttt ttcccagaca
     4861 tcagttttga agagctagtc attatcattc tcgatgaaac aagagaagct aagttaaaag
     4921 tttcggattt tatcatacag aatctgactt tacacttggc tttggggatc aaacggctcc
     4981 gagaagggtt tgaaatcaag gaattaggca tcgaacaaga agtttctaag cgagtagagt
     5041 atcaagtggc gcagcggatc gttcaacgta ttgaggtaat cgcaggtgta tcgtttcccg
     5101 aagaagaaat tgcttatttg acattacatc tcatggctaa gtcgaatcac aaagaaaatc
     5161 aaaatgatca ggaactatca aaggaattag cgaaagttct gaaaagattg tctcaggaat
     5221 ttcatttttc attagtagag gatctccagc ttttcaatgg actactcgat catttgtcac
     5281 cgatgatgat tcgattgagc cgaggtattc gcattgagaa cccattgact gaggagataa
     5341 agaaagaaaa tcccaaagtc tttgacgcag taaaaaggca tttcagcaac atgccggctt
     5401 tgaaaaacta taccatcaat gaagatgaat gggcctattt ggctttgcat ctcatggcgg
     5461 cattggaaaa agaaagagct gcgcataaac tacatgcatt gattatctgt gcaacgggtt
     5521 acggcagtgc ccaactatta aagaatcgcg tagtcagtga attcggtaaa aatattacag
     5581 tggtaagtgt aaaaggctat tatgagatca atgaaacaac tttaaaagga atcgacctga
     5641 ttatttcttc tattgatctt tcgacaatgt ttttcaaaat tccagtttta catgtcagta
     5701 tttttttgaa tgaagatgat gttcgtaaaa tacgaaaggt agttggagaa tctataccct
     5761 atcattcctc agatagaccg ataccagcat tttctctaca gcaaaaaaag cagatttaca
     5821 ccgaacagtt gtctgaaaaa tttttcaaga cttatacgta cgaaccgaaa aaggaagaaa
     5881 ttcttcatga tttgctgcaa aagctgagca taaatgaaga agaaggatat ataagagaaa
     5941 tgcgaaagca gatccagcaa cgtgaaaata tgggacaaat catattcagt gatacagtgg
     6001 cagtcccaca tcccgctgtt ccagttggaa tcgaaacaaa ggtagccgtt gcattgattc
     6061 cttctgggat gcagtgggat caatatcaag aaattcattt tgttttttta gtttcgcctt
     6121 cttatgtcga aaatgaagga attacggtcg tgactaaagc aatcgttagg ttagttgacc
     6181 agctaaaagt acagcaggaa atcttagctg aacctatttt tgagaatttc agcagtaaat
     6241 ttatcaaact gatcgaataa ggaggaaaac agatggctga aaaaataaac agtgaaacac
     6301 tacaagtgac ttcttttgat atcattttcc atagcggaaa tgcacggaca ctgatacatg
     6361 aagcatttcg ctcgatgcgt aaagagatgt ttgaagaagt agaagagaag ttgcgacaag
     6421 caaatgaaga gattcttcat gcgcatcagt cacaaactca acttctgaaa gaatatgcca
     6481 gcgggacaaa aatagaaatg gaaattatca tggtccatgc acaagatcat ctgatgacga
     6541 cgatgacatt attggaagtt gcacaggaaa tgaattattt gtatcaaaaa aataaataga
     6601 aaggaaggat cattgtgcag gatacagtaa aaaggaaaat cgaattagaa aaaaagcaac
     6661 ttagtttgaa aaacatgtat ttcaatcgtt atctaatcgt acgttacctg actgcctttt
     6721 tctttttcat gaacatgcaa tggatgattc tgcttctatc agctaaaagt ttaggaagta
     6781 gtttaccgat gattctgatt ttagctattt tgcctgcagt aggagaacaa gtaaagcttt
     6841 atcgaaaaca ccaaacgaac gttccatgga caaagtggta ttttttgttc cagagtgtct
     6901 ttaatttatt agtgattcca gtgttgttta cctcaggatt tacattcttg tatcctttta
     6961 tggcaaataa caatggagga cagttatttg ttttcattct tgttgtaagc ggtatttttg
     7021 ccagtgcatt tatacagtat cgtttaaaga aaatttcact caaccaagac caacagtata
     7081 tccgtattaa acaatatgaa aaagcgttat atttaggaaa ggagaataac taatgtttga
     7141 ttttttgcag aaatacctga tgggacctat ggggaaaatt gcgcaactta aaatcgttcg
     7201 ggctgtcatg gcagcgggga tggcttctat tcctttcacg attgtaggct cgatgttttt
     7261 ggttttaaat attttgccat taccgttccc atttttagaa ggatttttta atgcaacttt
     7321 tttcaaagtt agtgacttgt acatgatcgt caatacaatg acgatgggga ttttatctat
     7381 ttactttgcc attgtgtttg cttatgaatt aacgtcaatt gaacgagatg aacaaaaatt
     7441 aaatgttaat ccgctcacgg gggcattact atctgttttt gcattcttta tgtgtatccc
     7501 tgaattgatc atgactgatg gtaaaatcag cttgattgct tctatcaatg agacagaaac
     7561 tgtagtaaat ggtattagga tgggctcttt tgtcgaacgt ttaggaactt caggaatctt
     7621 tacagctatt attatgtcga ttattgcggt cgaattgtat tgtatgtgtg tcaagagaaa
     7681 ttgggtcata aaaatgccag atgttgttcc gccaggagtt tctcgttctt tcactgctct
     7741 gatcccaaca tttgtcattg cgtttgtggt gatgatcatc aatggattat tagttgcact
     7801 aggaacggat atcttcaaaa tgattgctat cccatttggg tttgttacgc agttgacgaa
     7861 tacatggctt ggtgtcatgg tgatttattt cttgatccat gcattgtgga tcgtagggat
     7921 ccatggagcg aatatcatca cttctttctt gaccccgatc gtattagcta atatggcagc
     7981 taacgcacaa ggcgcaaatt atccgttggc aggagaattc aacaattctt atgtaaccgt
     8041 tggtggatca ggtgcgactt taggattgat catctttatt gcattcctcg cgaaatctga
     8101 tcagctgaaa gtgttaggaa aagcctcact tgtaccagga ttattcaata tcaatgaacc
     8161 aatcattttc gggatgccaa tcgtctacaa tccctattta gcggttccgt ttttcttagc
     8221 tccgatggct tctgctacac tcgcttattt tgccattcgg ctggaaatcg tcaaaccgat
     8281 gcttgcacag atgccgtggc cttcaccagt cgggatcgga gcatttgttg gtagtggtgg
     8341 tgattggaaa gctgctgtgt tagcagtcat ttgtgcaatc atcgcattca ttatctggtt
     8401 cccatttatc aaattctatg ataataaatt attaaatgaa gaaagagaaa aggcagcaga
     8461 attagctagt gaaaagttaa aatgatttct aagaaaaaag gaactgtgac aaaaaatcac
     8521 agtccctttt tttcttagaa atgggaaacc actagtatat aaataccatt attcttcaat
     8581 caaccgcttc acgattgcat aatccccttc ataaggttca tctacaccat tgattttttg
     8641 atagggatcc acgccttttc ccgcaataac gactgaatct tgcggagcgc tttctgctaa
     8701 agcgcgtcga atcgcttcag gacgatccat ttcataatga acgatcaaat ctggattagt
     8761 aattgcttca ttgatttctt ccgcgatttt tttcggatct tcaaatgctg ggtcatctgc
     8821 cgtcaaaaag gcgatgtcag ctgtttctga tagcactttt ccaaaatcat gtcgacggga
     8881 gattgctttg tttcctgggc tgcctaatac aacaattaca cggccatctg gatgttcatt
     8941 tttggcaaat ttcagcaatg ttttcaagct tagataatta tgtgcatagt ccacatagac
     9001 atgcgctcca ttggattgga tcaattgatc cattcggcct ggtacacgag cttcttttaa
     9061 tccgtcttgt gcatcttgac tggaggcacc tgttagtgct gcggcaatga tcgcactggc
     9121 tgcattttct tggttgaatc ctccagccag caaaatatca taagcagtat cgcagatagc
     9181 taagcgatct tcatgagaag ataaagtaaa accgtgagta cctttatctg aacggatcac
     9241 ttgataattg ctttcttccg aacgaccata agtgatcgtt ggaatctgga atagatcggc
     9301 tgtttcccta agtaaatcaa aatagtcact ttcatgattt aaaacaactg tttttgaatt
     9361 caagatcaat tgtcgcttgc aatagaaata gtcatcaaat gtcggatgtt cgatcggact
     9421 gatatggtca ggcgagatat tcaagaagat tccgacatct aaagtcaagc cgtatacgcg
     9481 ttgtgttttg taagcttgtg aagaaacttc catcactaga tgagtcatac cattttcaac
     9541 tgcttctgac atcatacgat ataaatctaa tgactcaggc gttgtcaaat gggatttgaa
     9601 atatgttttg ccatctaacg ttgtattcat agtagaaagc aatgctgttt ttttatttgt
     9661 tgtgtgatcc aagattgctt ttgtaaagta cgcagcagtt gttttacctt ttgttccagt
     9721 gaatccgatc actttcaatt tattttgcgg atagtcgtaa aaagccatag caagaagcgc
     9781 cattgcttta cggatatccg ttacgatgat tcctactgct gaattttcgt atggttgttc
     9841 tgatacataa tattgaatac cctgttcgat tgctgaatcc aaatattctt ctttgaaatt
     9901 cattccttta cagaaaaaca aagtagaagc atcagctttt cttgaatcat aacttaactg
     9961 aagaaattca gctttttctg gtacatcaag atgccagcct cttgttgaaa caaattcttt
    10021 taatagattt tcttttaata gtaaatgacg gatgtcttct aaagagaagc tcattgattg
    10081 atccctcgct ttattcaatt tcttggttta ttatagcaca ctctttttaa tatatcgttt
    10141 gtttccgcta tgacaaaatc cagctttatt caaattttct tgacacaggg gacaagcgga
    10201 tgagtatttt cataaacaat gaactgactg taatagatag gagaacgaat agtgtctcca
    10261 acaatccaaa aacataataa ttcaaaaaga cgataagact attggtaagt aaaagtcctt
    10321 gcggcattgg aagccgaaaa aatttgtgga aaataatcgg cggtatcgtt ataccgccat
    10381 ttgaggcccc gactgtgtac aatgtgtaaa ccccaagact gaacaaaaga cttcctgaaa
    10441 tcagtgaaaa gaaatgtgag gagatcattt ccacttttgg aacaatctct aaaacaacag
    10501 gaaaaagcag acttcccgct acagtcttaa aaaatactga ttttcctaat aggacagcgg
    10561 ctagaagcag catcataaga ttgatgctcc aaacagtaac tgagcgttgg atagaaaagg
    10621 ccgtttcaac taaatgtccg atggcgccca ctccggcgga agctatatcg tgagggagca
    10681 agaaccaatt caatccaact gtgataaaga aaagacctaa aaatataacg ggtagttttt
    10741 ttatttcttg tgtcattgat atctcctata aatgttagcc ataaaaggct gtatatttct
    10801 ttactttacc aaaaaaacga aaccgaattc aaaaaaacaa ttgttcttat aaaagttata
    10861 taaaatcgat tgccagtttc tgttttacca attaaaatga tagtaatgtt ttgtagaaag
    10921 ttggaatctg accgatggat aatcaaagaa aaccagcatt agatcaatta acaccgcaag
    10981 aaaaaatggc acgaggctct gcttggatga ccgccagtaa tatcatttca agattgctag
    11041 gcgctgtcta tatcattccc tggtatgctt ggatgggcga aaatgctaag gctgccaatg
    11101 gattattcaa tatgggatac aatatatatg cattgtttct actgatctct acagcaggaa
    11161 tacctgctgc gattgcaaaa caaactgcac gctataattc attgaatgaa tatggcactt
    11221 cacgacgatt atttatccgt gcattacaga tgatgggcgg gttaggcctg ttgtttgctt
    11281 tgttcatgta catcgcctct ccttggctag ctcatgcttc tggtggcgga gaagaactga
    11341 ttccaacgat gcgttctttg agtatcgctg tattggtttt tccttgtatg agtgtgatac
    11401 gagggtattt gcaaggcaat caagaaatga tgccttatgc gctgtcgcag attgtagaac
    11461 aggtggcacg tattttttat atgctattgg ctacattcat catcatgaaa gtcatggatg
    11521 gtgactatgt gacggcggtc acgcaatcaa ctttcgcggc atttatcggt atgctagcaa
    11581 gtattcttgt ccttttgtat tttttgaaga aacatcaagc gtatacatcg gcgtttattc
    11641 actacagcga aaacaaaata aatattgcaa cgaaggaatt gttgttagat accgttaaag
    11701 aagcgattcc gtttattatt gtgggttcag gggtaacgat cttcaaatta gtcgatcagt
    11761 tcacttttat gaagatcatg tctgcatcca ccgagtattc caatgcacag ttactggatc
    11821 ttttttcgat ttttagtgcc aatccagata agttgacgat ggtagttatt gcattggcaa
    11881 catcgatcgc gtctactggg ttaccgttga ttacagaagc tgttacgttg aaagatcgag
    11941 taggtctggc aaaattgaca agcggaaatt tacagttgtt ttcatttttt atgtttccgg
    12001 cgacatttgg tgttatgtta cttgcctatc cgctaaatac attattttat acaccggatt
    12061 ctctaggaag taatgtactg atccaagcaa gttttgtagg actcttccta ggactatata
    12121 tgctagtttc caacatgctg caaggaatgt ttgaaaacaa ggcggcaatt aattatttgg
    12181 ttattgggtt tctagtgaaa ctccttcttc aatacccagc cattcgttta tttgaagtat
    12241 acggcccttt attggcgaca atgatcggat ttgctgtgtc ttgtaccttg atcttaaaga
    12301 gaatccatga agtagcccgc tttaaaccaa gttttgtctg gcgcaggaca ttgctgatct
    12361 ttattcttac cttgattatg cttttggctt cgtggatcac aaagatgatt tttggtgctt
    12421 ttttaaacga agatagcaaa ttccagtctc tattgttgat tcttgtagta gcaggtatag
    12481 gggctttagt gtatggttat ttagctttga aaatccgctt ggcagaaaga ctactaggac
    12541 caggaatagc gcgtttacga cataaattaa aaataaaata agtgaatcag aagagctaga
    12601 ttgatgctct tttgaaatgg gggaaacgca tgcgtttaga taagttttta gcagatatgg
    12661 gacttggcag ccgcaaagaa gtcaaaggat taatcaaaaa aggaacgatc gaagtcaatg
    12721 gacaagtggt gaaaagcgat aaagcgcaag taaatgaatt cgaagatgcg gttacttatt
    12781 taggagaaat cattacgtat caaaaagatt tttactacat tttgcataaa cctgcaggag
    12841 tcatttctgc tactcaggat aatcatgatc aaaccgtaat cgatttattg gaagaccagg
    12901 attatcggga agatcttttt ccagtcggcc gattagataa agacacagag ggcctcttga
    12961 ttttaacgaa tgacggggtg tttgcccatc agttactatc acctaaaaaa catgtggaga
    13021 aagaatatct agctgaggtt agcggtataa tgacagaaga agatatccgt ttatttgcag
    13081 aaggactgat tattgatggc gatgagcaga cattgccggc aaaactccaa attgatcaag
    13141 tagatcaaga gacagaaact tcaattatcc gtctgatttt gcatgaagga aagtttcacc
    13201 aagttaagcg aatggtcaaa gcagtaggaa aagaagtctt atatttaaaa agaatccgta
    13261 tgggaaaatt ttggctgcct aaagatttga ataaagggca atatcgaccc atgaccaaag
    13321 aagagctaga acaagtgaga gaaaaataat tgaatggata gctaaaagaa taagcagagt
    13381 agttgtagcg ccatctgaaa agcagacttg tccgtctgac tttcggatgg cttttttgtg
    13441 aaatttcggc ttatttccgt agaagctact tctggaacac cgtttgttcg gaataaggga
    13501 ctgtgacaag acttttgtta cagtccccat tgttccttgt gactcagagt tagatagtaa
    13561 ggacacgatc tccctaatac tcaggccaaa acgctgaatt cacaacctct gataaacggt
    13621 gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    13681 cccgtttttt tctctaatac tcaggccaaa acgctgattt cacaacctct cctatacttt
    13741 gtttcatttt ccctatactt tgtttattta ttctcattat tgaaagcgct ataattacgt
    13801 tgcgaatgaa tcgaaagggg tttttagatg aaaaaacgaa aggggttcaa tcattttttt
    13861 atgaatctat ggcgttataa agcacttgtc ctgatggctg taccgggtat gctttggatg
    13921 atttttttct tttacatacc tgtaatggcg aatgtcgtag cattcaaaaa ttttcatatt
    13981 tcagcagatg ggttcttggc tagcttgagg gaaagtcctt gggtgggttg ggaaaatttc
    14041 aaatttttat tttcttcgga tcaagcattt ctcatcacta aaaatacaat tttatataat
    14101 gtagcattta tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat tatgagcgag
    14161 ttgagaaata aacgactcgt taaagtttat caaacgatgt ccttactacc ttattttcta
    14221 tcttgggtca ttatcagtta ctttgtttac gcttttttaa gtcctgacaa aggaatattc
    14281 aatcagtgga tcactggaca tggaggagaa gctgtcaatt ggtataatga accgaaatat
    14341 tggccgttta tcttggtgtt cattggtaca tggaaaggga ttggttataa cagcattatt
    14401 tactttgcat ctgttatggg aatcgatccg acttattatg aagctgctat ggttgatgga
    14461 gctagcaaat ggcagcagat caaacatgtg acgataccac aacttattcc actaatgacg
    14521 attttaacga ttttagccgt aggaaacatt ttccgagctg attttggcct gttttataac
    14581 attcctagga attccggtgc attatatgat gtgactcaag ttttggatac gtatatctac
    14641 aatggcctga catccacagg agattttggt atgacggctg ctgcaggact ttaccaatca
    14701 gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt tgatagcgat
    14761 tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga gtccaagtcc
    14821 gctctttcaa taagacggct aattttttct ctaatataat gattgcactc tttgctattt
    14881 cctgtatttt gccatttata tttgttatcg ccatctcttt taccgatgag tccatattgt
    14941 cgactcacgg gtacagtttt tggccagaaa agttcagtat atttggttat acgtacttgt
    15001 tcgaccagat gcaggataaa attttccaag cgcttttcgt cactgtactt gttactgttt
    15061 taggtacact gatcaatagc acggctactt ctttatatgc ttatgcgatt tcacggtcaa
    15121 actttccatt ccgtcgtttt tttaccgttt tttgtttgat tacgatgttg ttctcaccag
    15181 ggatggtggc caattatctt gttatgacaa atcttcttca gttaaaagat acgatttggg
    15241 cattgatctt gccaatggct gtcagtccct tcaatattat tgtgatgcgc acattcttta
    15301 aacgttcggt ctctgattcg attattgaat cggcacggat cgatggagct agcgaactgc
    15361 gaatatttgt tcagatcgtt ttaccgttag ctgttccagg tattgcgaca atcagtttat
    15421 ttgccgcatt gggttactgg aatgactggt tcaatgcttt actatacatc caagatgata
    15481 agctagttcc actacagtat cttttgatga aaatccaaag taatattcaa tatctgacac
    15541 aaaattcagg agcaggaagc caattgacag gcggattagc ctcagttcca ggagaatctg
    15601 ccagaatggc gatcgtagtc atctcgacgt tgcctattgc gatcagctat ccatttttcc
    15661 agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga aacaaaaata
    15721 taaatcataa aattaggagg aagaaaaatg aagttatgga agaaactagc ctttacaggt
    15781 gtttcagcgt tgttgttggg aacattagca gcatgcggag gttctggttc taaagaccag
    15841 gcagaagcta gcaattcgga cactttgcag atgtatcaaa tcggagataa accagataat
    15901 ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg tgtaaaagtc
    15961 aatatcaatt acattggctg gggcgattac gaaaagaaaa tgaacgtcat catttcttcc
    16021 ggggaaaatt atgatatcgc ctttgcgaac aactatgttt ccaatgccca aaaaggagcc
    16081 tttgctgatt tgactgaatt agcgcctaag tacgctgaaa aagcttataa tgatttagat
    16141 gaagcatata ttaaaggaaa tctggtcaat ggaaaattat atgctttccc agtcaatgga
    16201 aatgtctttg ctcaacaagt acttactttt aacaaagctt tactagataa atacgatctt
    16261 tcaattgatg gaatccaatc ttacgctgat gcagaaaaag tcttgaagga attccatgaa
    16321 aaagaaccaa acacggcagc ttttgccatt ggacaaggct tcaaagtcca agggaacttt
    16381 gattatcctt tagggaatac gttaccattt gctattgatc tggatggaga tgattcgaaa
    16441 atcatcaacc aatacgataa tgaaacgtat atcggactgt tacgtacaat gcatagctgg
    16501 tacaaacaag gactcattcc aagtgacgct gcaacgagca ataaggatta tccactagaa
    16561 ggaaatactt ggctaatgcg tggagaaaca caaggaccgt atgattatgg cgatacaatt
    16621 ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc actaaaatct
    16681 acagctcaag cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa taaagaaaaa
    16741 tcagtagaac ttctagggct gcttaacagt gatccagaat tattgaatgg gcttgtttgg
    16801 gggatcgagg gagaagcatg ggaaaaagtc gatggttcag atgataaaat caaattgcta
    16861 gatggttatc agccaaatac acatatgtct gcttggaata caggaaacaa caagattcta
    16921 tatacacaag agtctattac ggatgacatg attgccaaac gtgatcaatc gattgctgat
    16981 gcagaaactt caccgatttt aggattcagc ttcaacactg acagtgtcaa aacagaatta
    17041 agtaatattt ctaatgtcat gaatcaatat ttagatggat tgaatacagg gactgttgat
    17101 ccagatgaaa cattaccgaa attgaaagac gctttggaca aggctggcta tgataaggta
    17161 ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag aataataatg aataaagaca
    17221 aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg tgttcaaaag
    17281 ccagtttctc ggtattaagc taaatatatg taaaaatgtg gaaagccata ttcacaaaat
    17341 ttttcttact actcgaaact aagcggctat ttcacaaact cttggtaaac ggtgttcaat
    17401 cagctaaccc tcggtattcg ctcacaaaac gacaaaatat gaggaactat ttttccgttt
    17461 gtttctctaa taatcaggtc aaggtgctga ttgcccaacc tttttttgta taatgagaaa
    17521 gtataaactt aatgtttgga ggagaagata tgttagggaa ggttttggaa gagttattta
    17581 tccggatctg ggtcattata aaactaacct tgtatttctg gatatataca tttgccggcg
    17641 ggatcatctt tggattaggt gcggcatgga aaacagtcaa tgaactattt tatttgtacg
    17701 gattcgagta taaagaaatc acaataaaaa gaggctggaa tatatataaa agaaattttt
    17761 taagaggcaa tctgctattt agtttatttt tatctggtac tgctttattg agttacaatt
    17821 tatatttatc tgttcaaatc aaagggctca tttttttagc tattgatttt attttacttt
    17881 ttgccttgct ctgcctattt gctacttatc aatacagtat gattttagat agtgaatata
    17941 cgatctctgt tcctaatctg ctaaagctaa gttttatttc ggttttttcc agcttttcgt
    18001 cttttctaaa aataattatt ggtagcggta taattttagg cgtgacttgg caattcaaag
    18061 gattgatcct gtttggtgtc attggtcttt tgacggtttg gaatggtacg atgacaacac
    18121 attggcgtga agaattagat aaacagttgg aaagttatga ataagtttcg gattctattt
    18181 tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg cggttatcat gatcggtgta
    18241 acgatgactg ctaccgttat ttttagtatc catacttggg aacaaaatca aaaacaggca
    18301 gaaaacatga caagtgatgc cgttcaaagc acaagcagaa tgctgaatga taaaacaacc
    18361 ttgagtagga tcatcaaaaa ccagcttgta gggaattccg aaaagatcga aaatgtgacg
    18421 acctacttaa caaaacccat agatcagtat ctgatgtatg tatacgaaca gcaaaacagt
    18481 accgatgaat tagtttcttt cccaaatcaa atcaaagatc tctacgcaaa ttatgaagaa
    18541 ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt attatgaatc ttctagagaa
    18601 aacaaaggtg gggaaataaa aaaggggaca ccaaaattac tagacgcctt ttatatcaag
    18661 ttgcctatca cacaaggagg agctgagtca ggaagtattt ttattggttt tcaaaagaaa
    18721 gaactagata tgattttaga aaatctaacg tcattcaatg gactctcttt atacatgatt
    18781 tctggaacga caaatcgact atacacattc catgataata aaatagctaa agaaacgttt
    18841 gaaagacaag aaatgatcat cacagagagt ctgaagaaaa attcacaatt gccgattgac
    18901 gatctggaaa aaaacaattt tgttcaacac caagaactgt ctgatgatta tcgtatatta
    18961 gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc cccgctgatc
    19021 attggtatca ttattttaga caatatactg cttatctttc tttataaaac attcaaacga
    19081 tattcacagc aagtatctat cgtcatgcag gccatggatc aaacagcagc agggaattta
    19141 gagacgcgga tcgacataac aaacacggaa tatgaactga aggaactttc aatcggtatc
    19201 aacgaaatgt tggatagcat caatcagttt gtagaagaca tctacaaatt agaaatcaaa
    19261 cagcaagacg cacatatgcg ggcgcttcaa gcacagatca atccacactt tttatataat
    19321 acattagaat atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt agcagatgtt
    19381 gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc aagagaaaac gatcacttta
    19441 aaagaagaac tggacttttg cgaaaaatat gtctatttgt atcaaatgcg ttatcctaat
    19501 cgagtagcct atcattttat gatcgatcct gatttggaaa agatcgaagt acctaaattt
    19561 gtgatccagc cattagttga aaattatttt aaacacggta ttgattttac tcgattcgat
    19621 aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat catcaaagat
    19681 aatggaaaag ggatgactga aaaacggcta aaacagattg aagaaaaact gtctcatcct
    19741 aaagtggaac ttcacggttc tatcggtctg caaaacgtga atgaacgttt acgtgccagc
    19801 ttcggcagca gttattatat gtcgcttgag aataatgaga caggtggact gaccgtttct
    19861 attacattca aggagggatg agcgtgtatc aagtgttatt ggtagatgat gaatacatga
    19921 ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt aggctttcaa atagtagcaa
    19981 cagcagagaa tgccaaagaa ggactagctg tacttgaaca gcgacagatt gatttagtag
    20041 taacggatgt gacaatgcca gaaataaatg gactggaatt tattgaagcg gctcaaaaag
    20101 aacgtcataa ttttgaattt atgattttat caggatacca agaattcgat tatttgaaag
    20161 gaggtatgca attaggcgcc gtgaattatt tgatgaaacc cgtgaataag ttcgaattgg
    20221 ttgaaagcct caagaaaatc aaaacacggt tagatcagca aaatgaacaa aaaaatcaac
    20281 aagaaatcta tcaagaactg ttattcagtc aatggttgaa tgaagaattg gatgaaataa
    20341 gtgaagaaga aataatagga caaataagtg agaagcagca taggatagta ttgattcaac
    20401 tggcaagaac tcatgatact cttatcaata gatggttgca agaacggcaa caaatctttt
    20461 actatcaacg aaattacgga gaccgtgtat tgatcacgtt gttgttggag gcagatgaag
    20521 ttgatggctt ctgttgtttt gttcaagaaa actttcctga tcaggaatgg ctgatcagta
    20581 tcggggaaga gacagattct ttggacaaaa tccctgaaac attccaacaa gcgaaagaca
    20641 atctacagtt gcatcaattt tatggagata aagacgagca tgttttttac gcagtacaag
    20701 cagaaacaaa agatcaaacc atcgattttt caagtttcag acgcgtgctg caaaataaac
    20761 aattggaagt cgctcaaaaa atgatcactg atttttttga acagtttcag ttagcagcca
    20821 tgatgccgga agatatacga tattccagtt ttttactgtt tatggagatt caacgggaat
    20881 tgattgatct ggaagatgag gaatatttac aaggaatcga aaaaatccat caggcaaaaa
    20941 cggtgcaaga acttcagcag ctacttctgt cattcatcaa aaagcatcag agacagaaaa
    21001 agtacagtga caatgtggaa aaagtaatcg taatcctgca ccagcattat caagaaccac
    21061 ttacactcaa agaagtgtca gaaaacctgc atttaaatgt catgtatcta ggacagctgt
    21121 tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa ccatttacgt atggaaaaag
    21181 cgaaacaact gcttttgcac agtaatcaaa acatcaatga gatagcaagt gagattggct
    21241 acaacaacac cacgtatttc tctaaattat tcaagaaaat cgtcggcaga tcccctaaag
    21301 agtaccgaga aaacagtgga gaatcataaa agaaaaagcc aatgtaatga aataacttat
    21361 aaaaaattgt ctagaaatct aattttgggc tgttcttatt acttgaggca aggcagcttt
    21421 ttcatgacct ctcgataaac gg
//
LOCUS       DACBZN010000040        21011 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.040, whole genome shotgun sequence.
ACCESSION   DACBZN010000040 DACBZN010000000
VERSION     DACBZN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.040"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            189..425
                     /locus_tag="IVD64_001467"
     CDS             189..425
                     /locus_tag="IVD64_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148725.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            449..790
                     /locus_tag="IVD64_001468"
     CDS             449..790
                     /locus_tag="IVD64_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148726.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(783..962)
                     /locus_tag="IVD64_001469"
     CDS             complement(783..962)
                     /locus_tag="IVD64_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP6148727.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            1184..1831
                     /locus_tag="IVD64_001470"
     CDS             1184..1831
                     /locus_tag="IVD64_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148728.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            2091..2435
                     /locus_tag="IVD64_001471"
     CDS             2091..2435
                     /locus_tag="IVD64_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6148729.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2440..2721
                     /locus_tag="IVD64_001472"
     CDS             2440..2721
                     /locus_tag="IVD64_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148730.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2824..3138
                     /locus_tag="IVD64_001473"
     CDS             2824..3138
                     /locus_tag="IVD64_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP6148731.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3116..4810
                     /locus_tag="IVD64_001474"
     CDS             3116..4810
                     /locus_tag="IVD64_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6148732.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4830..6008
                     /locus_tag="IVD64_001475"
     CDS             4830..6008
                     /locus_tag="IVD64_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6148733.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5971..6657
                     /locus_tag="IVD64_001476"
     CDS             5971..6657
                     /locus_tag="IVD64_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP6148734.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6657..7817
                     /locus_tag="IVD64_001477"
     CDS             6657..7817
                     /locus_tag="IVD64_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6148735.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7827..8702
                     /locus_tag="IVD64_001478"
     CDS             7827..8702
                     /locus_tag="IVD64_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148736.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8699..9010
                     /locus_tag="IVD64_001479"
     CDS             8699..9010
                     /locus_tag="IVD64_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148737.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            9000..9353
                     /locus_tag="IVD64_001480"
     CDS             9000..9353
                     /locus_tag="IVD64_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6148738.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9343..9744
                     /locus_tag="IVD64_001481"
     CDS             9343..9744
                     /locus_tag="IVD64_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148739.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9737..10141
                     /locus_tag="IVD64_001482"
     CDS             9737..10141
                     /locus_tag="IVD64_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148740.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10153..10758
                     /locus_tag="IVD64_001483"
     CDS             10153..10758
                     /locus_tag="IVD64_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6148741.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10778..11140
                     /locus_tag="IVD64_001484"
     CDS             10778..11140
                     /locus_tag="IVD64_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148742.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11143..11325
                     /locus_tag="IVD64_001485"
     CDS             11143..11325
                     /locus_tag="IVD64_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148743.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11342..14761
                     /locus_tag="IVD64_001486"
     CDS             11342..14761
                     /locus_tag="IVD64_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6148744.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            14812..15549
                     /locus_tag="IVD64_001487"
     CDS             14812..15549
                     /locus_tag="IVD64_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6148745.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            15559..17850
                     /locus_tag="IVD64_001488"
     CDS             15559..17850
                     /locus_tag="IVD64_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148746.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17874..19964
                     /locus_tag="IVD64_001489"
     CDS             17874..19964
                     /locus_tag="IVD64_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148747.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            20127..20573
                     /locus_tag="IVD64_001490"
     CDS             20127..20573
                     /locus_tag="IVD64_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148748.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20575..20712
                     /locus_tag="IVD64_001491"
     CDS             20575..20712
                     /locus_tag="IVD64_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6148749.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            20750..>21011
                     /locus_tag="IVD64_001492"
     CDS             20750..>21011
                     /locus_tag="IVD64_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148750.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQ"
BASE COUNT         7517 a         3085 c         4364 g         6045 t
ORIGIN      
        1 gttgtattat gatagtgtcg aaagattagg aaacaggatc gacaaaataa aatgtaaggg
       61 aggaaatctc cctcatcgtt taattaagct tcgataggca gcagcggaaa tattaagaat
      121 aaggatgtga atttcaactc cttctaaatt gttcttatta tctatcatcc gttgctgtct
      181 attaatttat gtattggagg gtatataaaa tgaagatcgc ttccgaaaaa attgatcaac
      241 taacaatttt agtaaaccaa agaatcgaag atattatcaa agaattcgaa ggtgaaaaga
      301 ttaagaacat aaagcttact ttaatggatc ataagttcga attaaattcg acaaaaagga
      361 agatcgttga gcttatagaa gaagaaatag ctctccagat caatgagcaa caattagcta
      421 attaatttat gtattggagg ggaaatgaat ggaagtcgaa caaataaagt atcaaggtat
      481 ggatcgggaa cggttgattg atattattga gcaacaacgt ttagaattga tccaaaaaga
      541 agctgtatgt aaagagtaca aaaaacattt ggaacaagta attgagtatc attcagtaga
      601 aaagtacaga tcagtggtgc aaaaaaatag agcaacgagt cctgattctc gtcaagatgt
      661 atcgattagt atcaagacgc cagtatttaa ttctgttaaa tcagaaaagc tagaaagtat
      721 ctcaactgct ttggtagaaa aagcagctga aatgaactcc ctagcaaatg aagttagtcg
      781 tcttatttag tcatatcgat aacttgagag tctttggtga ttccgttttt tatagaatta
      841 atggtttgtt cagcggaata tttagtcaag tatgtttcgc tagttgctac tacttcattg
      901 ttattggact taataacgaa gtaatattgg ccattagttg cttctcttat aacaaagtac
      961 aaatttttca ccacctataa tttatttcag cggaccactc gctgataatt aaaattataa
     1021 gctatgtata aaatttcaca atatcaattt gtcgctgtgg cggaaagggt agacgcaata
     1081 aaggatgaat gggaaaagtc aatgagtaat tatctgaaat ttacaagtcc cctgtttagt
     1141 tatctaagat tatttgataa tattatttct gaggtgatag aaaatggtgg gcgtagttaa
     1201 atttcttggg tttacatttg atggatatgg tacgttaggc gaatgggtag gtattatttt
     1261 gatagtgatt acaattcaac aaacatcaaa gtactactgg tattctaata gttctaaaat
     1321 tgtatcaaga ataactgaag gaataaccaa aaaagatatt actgtcgtcg gtaaatatgg
     1381 gtttactata actttattaa atgaagggaa atctgaaacc gcaataaagt tttgcgggat
     1441 tataggtaaa tataacattt tccaacgttt tatcagaaag cattggtaca aatatattta
     1501 caaataccct gtagttaaaa agcttattga aaaatatgtg gaccacgaaa tatcttatct
     1561 caatccaaca gaccttatta atcaagaatt ccagaatatt ggatacttag aaaaaggtct
     1621 aaaaatacca attgtaaatg aaagtttagt tactgcaatg gtagctaaca taaagaacag
     1681 caagaaatta gaaaagagat ataaaaaatg taaaaaattg aaattttcct ttttattctt
     1741 agaatataat ggcaaagaat attatcaaga tatttatgtt ttgcgaggct cgaacttata
     1801 taaagacatt aaaaataaga tttcagtatg agatttttta aaattcgtag atcactcttt
     1861 gagtggtctt tttattttgc gtaaaggagg cacgacgatg tatagaccac aatacttaga
     1921 acagaagcat gaagtaatca ctgtgcaaaa cggtaacggt gagatagtac gaaagtatag
     1981 aagaccaata aagagcgata catataaacg gaaggaaagc aatgaagtta ttccattgta
     2041 tggcaaaaga atagctaagt attaaataag attgcgaaag gagatgggac atgaccgagg
     2101 aattctatag atggttatta cagttgataa gagaagatcg tttggttaag ttctatcagt
     2161 ctcctaaatg gcgcaggctt agagagaaag cgatgaaacg agatcactat gaatgccaag
     2221 agtgtagaag actaggtaag tatcatagag tagagaacgt tcatcatata aaggaagtca
     2281 aggatagacc tgacttagct ttagatttag ataatcttat ttgtttatgt gttgaacatc
     2341 ataatgaagt tcatggcaga tatcttacag cgttagataa acaagagaag aagatagaaa
     2401 gcttcgctaa cttcgatgca agtgaaaggt ggtaagtgca tgatcatcaa tgacaatggc
     2461 agagagtatg atacagaaaa gattgaagag tattcatctt atactcaggg attaattaaa
     2521 cgtttgatat acgttcgcta tgtaggtatt agggatctgt tatcagataa ctgttgtagt
     2581 aaatacaaag tgaatcaagt aagagaagcg ttgaataaag ataataacgt cgaaagaata
     2641 aaaaatgttt ttggatatag tattgaagag attaattatt acattgactt cgctgaagct
     2701 ttcattccga tggtgagata accccccctt aaaataaatc gcaaattttt tgggggtgat
     2761 gaaacggagg gggctgtcag gaaaagagat tttttcgaac tttatcatga aaggagggct
     2821 aaaatgttta aaaacgaatt gtctcaaaat cgctacagag aaaaattacg ccgctcttta
     2881 ataagccaat tggaaagtca gaaaacaaat attgagccat tcttagataa tgttgatcgt
     2941 tatatcagtt tatgggaaac ggcgatatca ctggaagaag atatatccga gaacggcatt
     3001 agactggaga atggtaaaaa gaatgaatca gtagcgttgc ttgtttctgt caacaaacaa
     3061 atgggattga tgttggataa acttgccatt actcctgaat tggtaggtga agcaaatgaa
     3121 tcaattcctg agttataagc atattgaaaa ttggttcaaa gctatagaag aaggcactat
     3181 caaggtatgc aaagagcaat tattgctaaa aaattatcta gaagaaagag tctttactag
     3241 agaagatatt tacttcgata agcagatggt agaggattca atcaatatac cagcacaata
     3301 ctttccattc gaattaattc cgtgggaaaa atttctacaa tgttttattt atggtgttcg
     3361 atggaaaaaa gataaaacac tagtgttcaa tagatatctt tcattaatgg gacgtggtaa
     3421 tggtaaaact ggttttgctt cttggaacaa cttctttcta ctaaccgcta aacacggtat
     3481 taaaaattat gatattgata tctatgccaa taatgaaagc caagcaaaga ctagttttga
     3541 tgatgtattt aaagtaatta aagatcatcc tgatttagat aaaaaagtat ttaaagctac
     3601 gaaggaagtt attcaaaata tcgctacaaa cagcaaactt cgttataaca cggcaaatgc
     3661 tagaacaaaa gatgggaagc gaccaggtgc aaaccgcttt gatgaaattc acgaaaatga
     3721 agattattca atgataaatg tggctacttc tggtggtggt aaaattcgag attatagaga
     3781 attttatgat acaactaatg gtcatgttcg tggtggtccg cttgatgaca ttatagaaga
     3841 atcaaaaatg attctttctg gagaacttgg aattgacaag gatggagcag aattttctag
     3901 tttgtttcca tttatttgtc gcttggataa cgataatgaa gttgatgatc ccgacatgtg
     3961 ggaaaaagct tgtccaacta ttaattacaa tgcagattta aaacggaaaa tgtttcaaga
     4021 atactctcaa atgcaacgta atgctggttt aagacttacg ttcatgacca aacgaatgaa
     4081 cagacctatg gaagatacac gatttgctgt tgcttcatat gatgatgttc tgcatacgaa
     4141 agaaaaagaa tttcctgaaa aaatggatga agtgatagga acagtcgatt ttgctgatag
     4201 acgagatttt gccagcgttg ggttgctagg aaaatacgat aaagatgttt attttacaca
     4261 acatactttt atccacgaat cagcccttcg attacaaaac atcaaacgag aggttataga
     4321 tatttctata gatcaaggaa aatcacagat cgttcatgga aaaaatatag aagctgatta
     4381 tattgtaggt tggtttcttg aaatgagtaa taaatattat attaaaaaaa tcgctatgga
     4441 tatgtaccgt gcaaaaatat tgaagcccgc tttagaagaa gcaggtttta ctgtggaaat
     4501 tgttcgaagc ggatctgtta cacatggtat gttaaaagat ctggttgatg acctttttat
     4561 taatcaacgt ttattttttg gtgacgatgc gattatgcgt tggtattgca tgaatgtata
     4621 tgaagagcat atttctaatg gaaatatacg ctatgaaaaa atagaacctg aaactagaaa
     4681 aacggatggc tttttttcat tccttcatgg tttgaatttt ttagatgata tttatgattc
     4741 tgctcctgta acagtcacaa atagctcagt agaaaataca ggaactggat ttactcctct
     4801 agtattctaa cttgaaagga ggtgagaaag tggggatttt tcaaaaggcg gtaggatact
     4861 tcacaaaaaa agcaacggtt cctttagaag aatacttttg taaattgcaa gttgattttg
     4921 tgtatcgaaa atttgcgatt gaaacttgta ttgatttgat tgcaaatgcg atgagtaaag
     4981 cggaattcaa gtcatatgaa gatggaaaaa ataaaaagaa tgatctttac tataggctga
     5041 atgtagctcc taataagaaa aataatgcaa cagaatttag aaaaaaactg atcaggagat
     5101 taatattcta caatgaagta ttgatcgttt ctccgtctaa taattctagc gaaatattta
     5161 ttgcggatag ttgggatgtc acagaatatg cattgaaaga tgatgtgttt tctcaagtgc
     5221 aaattaacaa catagtcctt gatagagaat ttctagaaag tgatgttatc tatataaaat
     5281 acgcagatca acaaattagg caactagtcg atgcgtatta tcaagcgtat gggaaactca
     5341 tttctagtgc catgaatgtt tacaagcgct ctaacgctcg tagatacgta ctgaaaggga
     5401 atttattccg accgcaagac aatacaacac aagatcaaat caataaaatg atgacatcac
     5461 aatttaaggc ttttatggaa gctgataatg caggtgcggt atttcaatta caaaatgagt
     5521 acacattaga agatttcagc ggaaacttcc aaagcaattc aagagatata aaaaacttaa
     5581 tagacgacat ctttgagatg acagcagcag cgtttcacgt tccgaaaaac ctactaaagg
     5641 gagacatgag tgggttatcg gatcaagtgg acgctttttt aatgttcgaa atcataccga
     5701 ttgctgaact tattcaggat gcgtttaacg ctagtctcta tgaagtagaa gaatacttgt
     5761 cagggaattt tgtacgtgtg gatacaacta tgatcaagat tactagcttc aaagatttgg
     5821 ttgacgctat tgatgtaggc attagaaatg gagtatttac aatcaacgaa ggaagagagc
     5881 gcgttggaaa tgatcgctct gataaggcga tggcagatga aatatttata actaaaaaca
     5941 atcaacaagt atcgaaagga ggtgaggcga atgacgacaa tgaaaacatt tctagcagta
     6001 aagaatgaag gcgcagtacc gcaaattttt attcagggat ttattggttc tagttggttc
     6061 tttgaaggga atactgacaa gggaatcaaa aatattttgg atagtctagg tgatcaagaa
     6121 gaaattgaag tagtaattaa ttcaaacggt ggagacgtat ttcaagggat tgctattggg
     6181 aacttactta agtcaaataa agcaaaagtt aacgttgtga ttaacggctt agccgctagt
     6241 gctgcttcaa ttatcgcaat ggctggcgat actataaaaa tctacaacaa tgcacaattg
     6301 atgattcacc gcgcttccac atacggagaa ggaaatgtcg atgacttccg tacgattgct
     6361 gaccaactgg aatcaattga taaatcggta aaggcttcat ataaaacacg attcaatggc
     6421 acagatgaag cattgcaaga acttcttgaa aaagaatcgt ttatggatgc agaaacagct
     6481 ttgagttatg gattggtcga tgaaattatc gatgcagaaa atagctcagg tactgaagct
     6541 aaaaaagaac aaagcgttga agaaattttg aatgacgtta aagaaaaaag agcagaaaaa
     6601 attgctgcat ttacagcagc attaaataaa acatttggac aaggagatgt aaaataatga
     6661 cagttaaaaa tttaaaaggt gtaacagctg caagcgacca attgatgaaa gcttttaaag
     6721 atggtaacga agaatctttt agcgcagcta tggtaagctt atctaaggaa attcaggata
     6781 aaattttaga agaagcaaca gcaaaaaatc aagatcaatt agtattaatg aaccgtggtc
     6841 agcgtgtatt aactacacaa gaaacaaaat tctataacga agtagtgaaa aacgaaggtt
     6901 ttgcaggggt cgaagaatta gtgccagcta ctgtatttga acgtgtattt gaagatttag
     6961 aacaatctca tccactattg caaaaaatta cttttgttaa cacaactggt gtaacagaat
     7021 ggattgtgtc acgtggagtc aatccagcat ggtggggtaa actttgcgaa gctgttaaaa
     7081 aagttttaga taatggcttt gatgtaatta acatgaagca gttcaagcta tcaggttata
     7141 ttcctgtatg taaggcaatg cttgatttag gtccagtatg gttagatcgt tatgtccgta
     7201 ctgttttagt agaatcattg agaattgcat tagaacaagc aattgttgat ggtactggta
     7261 aagatatgcc agtcggaatg atgcgtgaca tgagcaaaca aactagcgga gaatatgctg
     7321 aaaaaaaagc agaacctatt acagctttag atgctgtaac tatgggcggt ttgatggcgc
     7381 gactatcaaa attcaatatc gaaggtgtga atgatccgat ttatcgtaat gtgaatcctt
     7441 ctgatgtggt cctaattgtg aatccaactg attactggtc taaagtattc ccagctaaga
     7501 ctgtactaac tgctaatgga gaatacgtac aagtattgcc agtaccagtt tcagatttgc
     7561 agtcaacggc tgtgccagaa ggaaaagcag ttattggggt agcctcagat tacttcatgg
     7621 gtgtaggatc tacactaaaa attgaagctt cagatgaata ccattttgtt gaagacgaac
     7681 gcatttatct agctaaacaa tatgcaaacg gtcaacctaa acgtaacgat agtttcattg
     7741 tattagatat tagcgctttg ggaactacta ctacaactac aaaaccaaca accacaacaa
     7801 ctacaacaca agcgtaggtg atcagaatga agtatattct ttgtcagccg gcaatcaatc
     7861 ggtttaaatg ggagcttgaa gtttgtttaa ctaatctgaa gaaactagga atcaaagata
     7921 tcgtattgct tttcagcaga cacgatgatc agattcctat tttttttgag aaggaatatg
     7981 gtgttgaagt tcatgtgtac gatgatctgc gggacgacaa agagtatatt ccttcgatta
     8041 aaccatattt atggtggaaa tatttagaag aagatcattc gcgtgaggac gaccgatatt
     8101 tctatatcga ttcggatgtc attttcaata aaagaattaa tttgcgcaaa ttgccttcta
     8161 aagatgatgt ttggtattgt agcgactgct gtagttatct aagtcttgat tatattagaa
     8221 gctgtgaaaa cggagaaaat attctaaaag atatggcaaa cattgtaaat gttacagtag
     8281 aatctttgga aactataaac actaattcag gaggcgcaca gtgggttatt aaccgcccta
     8341 aagcgaatta ttggaaaaag gtttatctgg attctaatcg gctatatcgc taccttagag
     8401 ggcaaaaaac aaatatacaa atctggacag ccgagatgtg ggcacagctt tggaacatga
     8461 tgtatttcaa tattggtcct aaagttcacg aggaattaga cttttgtttt gctactgatc
     8521 caatagaaaa agttaaagaa gtaaaaatct tacacaatgc tggagtaaca acaaacgatg
     8581 aagatttatt tttcaaaggg agatacgtga cttctacgcc ttttgatgaa gatttatcat
     8641 ttgtaaacaa gaaaaaatgc tcttacgcat atgttaaagc aattaaggcg gtggttagat
     8701 gacgcctgaa caagtgactg aagaattgct aatagctgtg aaggataata tttacgttac
     8761 ctggaacgaa gaagatgagt caattaaaaa gatgatagct aaaaatgctg tttatcttca
     8821 aagtaaagtg agtacaacac tttctttttc tcctgaaagc ttagaatacg gattgctaat
     8881 cgaaagatgt agatacgact ggaatcgtgc tttagatgag tttgaacaaa atttcgctag
     8941 tgagttatta ggtttcattc aacattatgc gctacaagaa tatattgcag gtgatgtgaa
     9001 tggcgaataa tcgtagactc gaagaaacat tcaacgatgg ttggttaaag attttgacgc
     9061 aaactaccaa aagaaatgaa ctaggaaaaa agattggtgt agaagataca gaaatcactt
     9121 ctttaaaatt tagaaatctt tccatgagag atagtgatat aacagctatg gatgcgatgg
     9181 gatcgaaatt aactaagaaa gtaaagactc catttcatcc aatcgccaag aaatttaata
     9241 aagatcaata ttttatcgta atcgatagta tgcgttacaa cgttatctat gccgattacg
     9301 ataattttta tatctatttt tatcttgaaa gtgtgggtga atatggtgat tgataattct
     9361 aaagaaaaag aacgtttaaa taagcaaatt tctgctatca aaacttcctt agaagagcat
     9421 tttggcctca aactctttca agactccgta ggcgaggatg agctacctga tgattttaat
     9481 tacttcattc tcgaaacagg agaaatagaa atgatcactg agccaaaata tagcgtgggt
     9541 caaaatctat atctaacttt ctattcagaa aatagagaag atttaacagg agattcacta
     9601 gatattattt cattgattca aaatcgttcg attcgttttc agagaatgga tcccaatcat
     9661 ttaaaactag agaaccaaga tcgctatatc gatcaattgg tatttacgtt tagacgatta
     9721 ttgaagagtg attgtcatgg ctaaaaatag ttgggagcta aaaataaatg gacatgatga
     9781 acttcttgtg cggatggaac gctattcaag cgagagcgaa cgactgatta acgaagcatt
     9841 gaaatcaaaa ggttcggcta ttgcagtgga taggattaca gaaaaaattc ctgtttctga
     9901 agcagattta agaagagggc accaacacgc aaaaaatagt cgtccactta agactcaata
     9961 cattaatttg ggtttcatca ttagacctac aagaaaattt gagtatttaa aatatcctga
    10021 tttggggata ggtacttcta aaagaaatca gccggacgaa tttatgagaa gaggattagg
    10081 tcttgcactt gatccaatta cagaacttct gattcgtcaa ttcgataaat taaataaata
    10141 ggaggaacaa caatggctaa aacaacaaca gtaacaactt ttgacaatat aagtattaaa
    10201 agaatttctt ttaattttaa gaatgcaaca aatgcaattt caactgattg taatggacaa
    10261 ttagatggcg aaacagaaat gcagagaatt gtaaaaaaat gcggttcaac agaagtaaaa
    10321 tcgaaatcta aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt
    10381 tatcgacgct tcaatgggtt gaaacaagat gaacgtatta aatcgggaat ttactcttat
    10441 ggtcctgatt ccgtagggga agatttctct cttgctgcag agatcgtgga tgatttcgaa
    10501 gaaaaaaata agctgattgg tatgttagca tgcacttcga atacaggatt aacattctct
    10561 attgaaaatg gtgcggatga agtagctgcg ttagaactag aaacaaaagt tatgcaagat
    10621 gaatttggta aattttatca tgaagcaatt gttgcagaac ttgaagaaga cttaacagat
    10681 caatggatga cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt gacaactacg
    10741 gccactcaat cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt actcaaaaat
    10801 tgaactaaac gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt
    10861 gatgatcaat agagatttta acacagacga gtttgcaaaa atgactgtgg gaaaaggatc
    10921 catggatatt tctgttattc aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa
    10981 catgactgat tatatttcat tcgatgaatt tatcgataaa tgggattttg atatggctac
    11041 tgccagctat atttatcaat tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga
    11101 atttgaaaaa gcaaataagg aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt
    11161 gaaacgtggg tcgatgtcta ttcgatgttg accgacgttt tttctatgcc ttcagatttg
    11221 gttttaagcg atatctgttt agatgacatt ttgcaaatgg cttacaacaa gagtgcttat
    11281 gaaggatgga aaaactatgc aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa
    11341 aatggctaaa aagagaacag aagcagaagt aacattcata gctaacgatg acggattgaa
    11401 atctacgtta aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga
    11461 acaagctcaa ttacaacaga ctggttctga atcagacaag ttaggaagta aattatcttc
    11521 tttagaaaag cagtatgaat tacaaagtca aaaagttgaa gtaactagcc aacgtttagc
    11581 caatgccaaa aaatattatg gagaaaattc caccgaagtt cagaaacttg agagagaact
    11641 gattaaccaa caaacagcgc aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc
    11701 actagctcaa gcaaaaggcg aaatacagac gtacgagtct acaatgcaac agttggatag
    11761 tgaacaaaaa aatgttcaag ctagtgcttc tctgattgaa tccgaataca aaaaatggca
    11821 agcaactgct ggtcaatcag cttctgaatc cgagaaatta gcgaaagccc aagaatatgt
    11881 ttctcaacaa tctgaaaatg cagagaaaac gatagatatc ctgagacgac agttagaagc
    11941 tacacagtct gagtttggcg ctacatccac agaagcaatg cagatggagg cgaagcttaa
    12001 tgatgctgaa cgtgaatttg aagagttagg acaagctgct aaaaatgtag atacaactaa
    12061 cttggacgat atcggaagca aaatagatat gaataatcta atggaagctt ctgacgtttt
    12121 aagcgacatt ggcgataagc ttacagaatt agggaaacaa gcagtggact ctgctaatag
    12181 tgtaggtagt tcccagagta aaatacaagc taattttggt ttgactaaac aagaggctga
    12241 agaattaacg aatgtagcca gagacattta ttataaaggt tttggagaat cgttagatca
    12301 gtccacagat gcattgattt tggtaaagcg taatttaggc gatttaaata atcaagattt
    12361 acaaaatatc acggaacaag ctatggtcct agaaaacacc atgggcgctg atatggatga
    12421 aacgttacgt ggtgtaaatg gcttaatggt caatttcggc ttgagcgctc aagatgcaat
    12481 ggatttaatg gtttcgggta ctcaaaacgg tttagataaa acgcacgaat taggcgacaa
    12541 tatggcagaa tatagccaat tatggagtca aatgggatat tcagctgatg aaacgttcgg
    12601 aatgcttcaa aatggtttag atgcgggtgc ttataacctt gataaagtca atgacttagt
    12661 taaggaaatg ggaatatcgt taacagatgg tcgatttgag caaaacatgg atatgtttag
    12721 tgaaagtact agaaaagctt ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt
    12781 tattaattcc atgattcaag attttagcaa tatggatggt caatacgacc aattaaataa
    12841 agcttctaca atttggtctg cacttggcga agataacgcg atgaaagttg tccaatcttt
    12901 gactgatgtt aaccatacat ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga
    12961 ttctactact ccgttacaag aattaaacgg aaaaatagct gaattaaagg attcattagc
    13021 tcctataggc aacacaatca tagatgcact cgaaccagta attgattttc taggaaagat
    13081 ggctgatgcg tttaataatc ttccacaacc agtacaggat tatgccgtag cgattggcgg
    13141 attgactgct gcatttactt tattaatgcc aataatagtt ggcttcatgg ctctaggtgg
    13201 tcctactaca ttaataatag gagcagttat tactgttatt gctggagtta tagcaattat
    13261 aaaaaactgg ggcgcaatta ctgactggtt taagggaata tggagtaaat tcactgattg
    13321 gttgggtggt acttgggaaa gtataaaaga aggtgcctca tcagtttggg atggagttaa
    13381 agaaacctgg tctggatttg tagattgggt tcaagatatt tggcaaggag tttctgattg
    13441 gtttggagag ctatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca
    13501 agagacttgg caagcattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt
    13561 atggtcgatt atttgggcag acattgtagg aattgttcaa ataccatgga ccttaataac
    13621 gtcattgatt caagccggta ttaatattat cgtgggtatt tttgatgtag ctggacagtt
    13681 attaggcgca gcttggcaag ctgtttggac acctatttct gatttcctta aaaacacttg
    13741 ggatactatg acacaatggg taagcatcgc ttggaatgga attgtaacta cattccatac
    13801 tatatttgat ccagtagtgg catggtggaa tggtatatgg acagctatta gtactacggc
    13861 ttcaaatatt tggaattcaa ttagtgcaac agcttctagt atttggaaca gtatcaagaa
    13921 tacaatcact agcttggtac aagcagctgc tacagtaatt caaaatattt ggtcaactgt
    13981 atctagttgg ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggaa
    14041 tagcgtgact agtagtataa gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt
    14101 ttggaatagt atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa acactgcttt
    14161 gaatctttgg aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc
    14221 aatttcaagc ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa
    14281 aagtacaatt gctaacattt gggaaggtat caaaaagact gttagcgctc caattgattg
    14341 gatcagagat aaaatcagta gtatctttga taatttgaat atttctatac cacatattcc
    14401 gttaccacat tttaaattga gcggagaatt caatccattg aaggggaaaa tcccaacgtt
    14461 gggtgttgat tggtatgcga aaggtagtgt gtttaattct ccgaatatta tcggtgtcgg
    14521 cgaagcagga cctgaagcag ttttaccttt gaaaagatct gtgctgcaag aaattggtga
    14581 tcgtatcttg agtagcacat cagtttcatc tagggcacaa acgattcaac ctgtgaataa
    14641 ctacgaattc aatttcacaa ttgatggtaa cgcagatgag gttactatga agcaaacaac
    14701 tcaacaaatc attgatagca ttacaaaagt tcaaaatgat aatgcttcgg catggcgtta
    14761 aacaggagag tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat
    14821 tgtatacatt ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa
    14881 agaccaacca taccttctcc aaaaagacag agagttgaaa cttctgtttt aggaaggtta
    14941 ggtggcttag tacaagatta ctcgtttgaa gacatgtcgt ttacattgca ctataactat
    15001 ttagaggatg tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg
    15061 ttaaattatg caaagaaatt agaattctct gatgatccca acgtctatta tgttatccag
    15121 actatcgata ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat
    15181 attactgcga aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca
    15241 cagtcattta acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact
    15301 ccttcagcta cttcatgcca gttcatctta aatgattatg tgtttagttt tgaaggctta
    15361 gtagtaggaa ctgacgtagt cattgatagt gatttgatgc tttgctacga agagcaatcg
    15421 gacggagata ttttagatcg gtccaacaaa atgaagacca tgcaatatcc gacattgcaa
    15481 gtggatatta attattttaa ttgtactggt ttgagcaaaa tacaaattta tcgtaatggg
    15541 ttaaggtagg tgaaatagat gatcgataat ttaataacta tttacgataa aaacgacgcg
    15601 aataatttag ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta
    15661 acagctactg ttagcaataa attaaacgga gccgagatat ttcagggtac ttatccaata
    15721 agcggaacta atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa
    15781 aatcgagcaa aacagcgtct acggatttat tatgcaaaga cttctgtaat aggcaatacg
    15841 atagaagtaa aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat
    15901 gacagtggaa cagaaaagat cactgctagt caggcatggc aaaacgcaaa aactttagcg
    15961 aaaccagtta ttccttcaca gttttctttc tcgtcattag tagatacgct tgctaatgtg
    16021 aagatagaaa aggcgaattt tttagaattc tttggtggaa aagagggatc tattctagat
    16081 cgatttcatg gggaatttct aaaagataat aacacattac gtcatgaaaa aaggctaggc
    16141 acggatcata aaatcaaagc gatttatact aaaaacttaa ctggtcttga cttagagatt
    16201 gatgctcaaa gtgttttagt tggagtttat ccattcatta gcagctcttc agaaggagaa
    16261 gacgagatca ctctaccaga agaagttatt ttcacggatt acgtggatga ttatcctgct
    16321 ggatatgttt cttttgttga ttttaaagac aaagcgactg atgtagccac attaagggaa
    16381 gctgctaaag actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg
    16441 attgaattag taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa
    16501 gtttcgatgg gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagcg
    16561 agaattgtgg aatatacgtt taatgttcta accaattcat acgataaatt agttgtagga
    16621 aacgtcaaaa caaacttctt agaaaacaca gagaataatg tcagcaattt gattaatgat
    16681 gccattgatc aattgaaaaa cggtggcgaa atcagtgatt tactcaatga tattgtagat
    16741 catcaaactg atatgattac tggtcaaaat ggtggttatg ttttattaga tcctaaagaa
    16801 gcgcctagtc gtattttgat tatggacaca ccagataaga ataccgcaag gaatgtttta
    16861 caaatcaaca atgctggtat tggtttttct aaaactggca ttaatggaac atatgaaacg
    16921 gcatggacgt tagatggcgg attcaatgcc tcgtttatta cggctggtga aatagtagga
    16981 attactatta gaggtactac attaattagt gatggtgctg attatagaac aagtattgct
    17041 aatggcaaaa tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca
    17101 cgtgattatg taagtgctga tgccggtatt gtatcataca ccatgaaaac tggtggtggt
    17161 ttcatgatta gaaatccaca gggtaacttg gtttttagta cgtgggataa tggtaataac
    17221 agaccgtttc tatcttttgg tgcgcccaat ttcaggtata gcaatgctag ttatgtaact
    17281 tctggcgacg gtagttcttt aagcattaat ggtagtgcgg gtaactcatg ggaatttaag
    17341 gtagctggta ggactatgaa atttactagt gatggtatgc taacgttacc aggttgtttt
    17401 tttggttcat gggaagatgg gaaacttgct aggtttgaac aatcaacggt acaagtatat
    17461 aaagatttta ctgttagagg tactaaaaac tcaactgtac caacagaaca ttatggacaa
    17521 cgactattga acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt
    17581 acaggtgacg atggtaaagt tcgtgttgat attgacccca tgtttgctga aacagtaaat
    17641 ctaagtcggt atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat
    17701 gaagatattg accatttcat cattgaaact agtaagccaa acgtattagt tagatggaat
    17761 ttagtggcac accgtctagg gtatgaagat attagattaa aagaggatac agcatatgat
    17821 agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta
    17881 gcagtttata taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta
    17941 tggctcgtca aggtgataaa gggattacgg tgacggttaa accgtttcta aatggcttgc
    18001 agatggatac gagtggcgga acatttactt taaaaggaac aacaccatct aaccgttacg
    18061 tagataatgt tgcaactagt gtaactagtg aagaagtcac gttttctctt gatggcacat
    18121 ttatgagtga agcaggatat tataaacact gctacgtaga atatagaaaa gacaatcaaa
    18181 ttttaacaac gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc
    18241 aagccgatga atatgtttcg caattagaag agttgattcg aaagtacaac gaaacttttg
    18301 atgcttttat ggctgaaatc aaaggtagag tggatagctt aaatcaacag attactgatt
    18361 taactggtca agctaaaacg ctacaagaca agttagatgc tctgaaagaa gaaatttcta
    18421 agttaggtaa cttacaagtg atgtacagta acagcatcga cttcggggga tatgattata
    18481 gcggtaagcc gaatttaatg tccaaactaa aatcgagcga ttttaacgtt ggttaccacg
    18541 ggtcactaac tttggataac gaaaagttac attttacttc tgatggtaca ggaagcatta
    18601 ttatgtttac gcgtattaat acacctcagc ttactagtgg gaaaacctat actctgagtg
    18661 cgaaagttcg atttgatgaa ggaactacag gagctattga taaattacgt ttggtgtatc
    18721 gtacatcacc aggagaaaag atattattgg aagcaaatag tacaaatatt acaacagatg
    18781 atgtagggaa agaaataaca atcaaaggta cagctaacgt taattatcaa atcacaaatt
    18841 tagatcgatt ttatatgagt attagctttg ttgacaggga taaaataaat ggcggattta
    18901 agttgtacga catcaaaatc gaagaaggct caacagccac accgtaccag cctaatctac
    18961 ttgatgcacc gtattatttg agtaaggtgg ctttgggtga gaatctaatt aaaccagaat
    19021 cacaacaacc agttactaat agtaactatc ttattaacac ctataacatt aaaccaatgg
    19081 taaaaggtaa gaagtatacc atcacacttg aaggaactaa gccagcaaca caggttttta
    19141 gaccattttt cacacgagca acaggagatg catgggaggt tggtgactta caaccagtag
    19201 aaggcttaac taatgtgtgg tctaaaacat ttacagcagc agatgactca caccctacta
    19261 ccccacaagt gcagatttat caagtaccaa gcacaagtgt aggacaatgt acaattaagt
    19321 ggttaaaact agaggaaggc aatactcgaa ccccgaatat tagtgagtat aaatatcgtg
    19381 gtactggtat gcgtgattca aacaatccaa aagattatgt ttgggatcta gcaccagaat
    19441 atgtcgaaga taatttggcc acagatatta aaatttctga aattactggt aaagcaaaca
    19501 attataccga tgggaaagta tcggagatta attcgcagtt gactgcttca attaatgaag
    19561 tagacaccac agctaaggat gctcaaacaa aagcgaatgc taatgcgact gctatagatg
    19621 aattagacaa taagatcgat gaacgcatta atgatacagc tactaccaca ttaacagtta
    19681 caaacgggaa taccggatca gcaaagcttt atcgtgaagg aaaaacagtt tctatatatt
    19741 ttgtggcttt aaacggaaaa cgcagtggtg gaaatgattc aacgatacta acaattccag
    19801 aaggctatcg gccaccaatt agttttgagc aactggttgg ctcgatagac cgttctactt
    19861 tgaacagtgc tcagttatct attggtgcag atggagccat taaatggcga agaaactcaa
    19921 gttatggatc ggattatacc tttgcaatta cttacacgat ttagaaaagc gtgaatcgat
    19981 atgaaggcag catatagacc aattgaacct tacggattcg agcaaatcat tgtgaatgat
    20041 gaagaacatt taccggaaga atgcacagaa gtcgaaccac cgattccaaa ttggaaaccg
    20101 aaattcaatt actgggaggg aaataaatga aaaacatttg gaaatatgga cgtactggcg
    20161 gagagtacgc aggaaaagta ttggacgaca tgcttgtatc cgttccttac acagatcagc
    20221 caccgcttga aggaattcgt gctgatggcg aaccgttaac aatcgctgat caaatgtttg
    20281 atcctaaatt gaatcaatgg attattttag cgaacgcact agatcacaac gatttaaaca
    20341 atctcaaagc aatgtatgag tcgttagaaa atgagaacgg cgatttaaaa cagatcaatg
    20401 ccaaactcat gctaagcgat gtagcaatta aacaggaaaa tactgcattg aaagaaaaag
    20461 cggatagttt agcacaaatc aattcaaaaa tgatgcttgc ttcgttacaa aatagcaaag
    20521 acatttcaga aattaaagag caactaaatc cagcttcaaa gggaggtgag tagtatgttt
    20581 agttttagcg atgtgaaaat gatgtatgat tggggctgtt tcactaacga acaagtaatg
    20641 gttttcgttc cgttgtgcat tactgaagaa aaagcagata aaatcattag caaagaagag
    20701 agcgcatctt aattgatgtg ctttttattt tgattcaagg agttgtcaca tgattaattt
    20761 aggggaatgg ggagcgatag caggatcaat aaccgctatc gtttctttga ttttattagt
    20821 aataaaacca attactgcat ctttctcgaa gattactgag actctttcaa aagtaagtca
    20881 caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca
    20941 tgaagaacta aagaaacacg atgaaagatt agatacacat gcagaaaaat tggtagaaca
    21001 cacgcaacaa a
//
LOCUS       DACBZN010000041        20742 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.041, whole genome shotgun sequence.
ACCESSION   DACBZN010000041 DACBZN010000000
VERSION     DACBZN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.041"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(5..841)
                     /locus_tag="IVD64_001493"
     CDS             complement(5..841)
                     /locus_tag="IVD64_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6148751.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(838..1707)
                     /locus_tag="IVD64_001494"
     CDS             complement(838..1707)
                     /locus_tag="IVD64_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148752.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(1777..3060)
                     /locus_tag="IVD64_001495"
     CDS             complement(1777..3060)
                     /locus_tag="IVD64_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6148753.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(3074..4759)
                     /locus_tag="IVD64_001496"
     CDS             complement(3074..4759)
                     /locus_tag="IVD64_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP6148754.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            5034..6059
                     /locus_tag="IVD64_001497"
     CDS             5034..6059
                     /locus_tag="IVD64_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6148755.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(6654..10307)
                     /gene="rpoC"
                     /locus_tag="IVD64_001498"
     CDS             complement(6654..10307)
                     /gene="rpoC"
                     /locus_tag="IVD64_001498"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP6148756.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(10386..14003)
                     /gene="rpoB"
                     /locus_tag="IVD64_001499"
     CDS             complement(10386..14003)
                     /gene="rpoB"
                     /locus_tag="IVD64_001499"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6148757.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            14731..15699
                     /locus_tag="IVD64_001500"
     CDS             14731..15699
                     /locus_tag="IVD64_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6148758.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            15717..16622
                     /locus_tag="IVD64_001501"
     CDS             15717..16622
                     /locus_tag="IVD64_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148759.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            16619..17425
                     /locus_tag="IVD64_001502"
     CDS             16619..17425
                     /locus_tag="IVD64_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6148760.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            17582..18550
                     /locus_tag="IVD64_001503"
     CDS             17582..18550
                     /locus_tag="IVD64_001503"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP6148761.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(18867..19391)
                     /locus_tag="IVD64_001504"
     CDS             complement(18867..19391)
                     /locus_tag="IVD64_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP6148762.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            19678..>20742
                     /gene="scm"
                     /locus_tag="IVD64_001505"
     CDS             19678..>20742
                     /gene="scm"
                     /locus_tag="IVD64_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6148763.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         6057 a         4385 c         3911 g         6389 t
ORIGIN      
        1 ctttttatcc ttttacgccg ccttcgacta aatcaccttt aataaatcgt tcagataacg
       61 catacataat gatgactggt aaagcggtca ccaatgaagc ggccatcatt cgtccccaaa
      121 tgtagtctgg tgtactgaat aatgtgttca aaccgattgg taaagtaaat ttttcggagg
      181 tcgacaggaa aatcgaagca aataaataat cattccacga aaccataaag caataaacga
      241 agacagaaat gattccagaa atagccaatg gtataatgat ccgaataatg atctggatcc
      301 ggctaagacc atcgatcatt gctgcttctt caatatcagt cggaatcgtg tcaaaataac
      361 tttttagcat gaatatcgca gtaggaagtg tttgaacgac catcgtgata atcagtgctg
      421 tattggtatc atacaaacct agaccggaaa tgattttaaa taatggaaca acgatcaaaa
      481 taccggaaaa catataaacg gtgtaaaagc tagcattgat tttcgagcga gctttgaagc
      541 gtagttttga caaagcgtat gccccgctga tcccaatcac tactgcagtt acggaggcta
      601 tcaaggagat cactaaacta tttttgaaat acatgatgaa tggaaaaatc tcagcattga
      661 aaatatcctg ataatgttca aaagtccatt ctttcggaaa aatagtttgt gtcgttgcaa
      721 ttgcttcttg tgaatccttg aatgatgtca tcagcatgat caaaaatgga aacaaattga
      781 cagctaatag caacaaaatc gtcgcgtaaa ataagatttt cctgctcttt ttatttttca
      841 tttcaagtcc accttctttc gtgagagcaa gacgaccgct gcaataatga tgaacaatag
      901 aatcgatata gctgctgctt ttccaagatc attgaacgca aaggctgtct gatacaaata
      961 aacgcctaag atattgactt tattcgtcag tagatagaca tctgtaaaca tatagaacat
     1021 ccagatagat cgtaaaacaa tcacagttgc tagtactggt ttgattgccg gcagtgtcac
     1081 gatcttgaac ttctgccaag catttgcacc gtccatttca gctgcttcat aaagcgtttt
     1141 gtcgactgtt tgcaaaatag ccaaaaatga aataaacgca tacggatagt atctccagat
     1201 agcaaacaaa acgaccataa agaagctagc aactggctgg tcgaaccaca gcggtgcttc
     1261 gttgaaaagc tgcaacctat ctactaacag atcattgacg attccataac tattattgaa
     1321 catatatttc caagcaaaaa tcaaagaaat agatggcgta acataactta ataaaatcag
     1381 tgaacgcacc gttttcctaa atctgaactt gcggttaagc aaaatagcta cggctaatcc
     1441 taaagctgta ctgcctgcta caactaagaa tgtgtaagca actgttaaac ctaaagaacg
     1501 ataaaattca ctatcactta aaatttcaag ataattgctt aatccgataa aaacggcgtc
     1561 tgagttagga ttcagcggca tatcgaaaaa actgatctga atattggaaa gcattggaat
     1621 cagcactaaa acagctaata aaagcatgct tggtgctaaa agtcccatgg ctaattttaa
     1681 gtcagaatgt ttccttggac gattcattga aacctccccc tgataatctg ccaatagcta
     1741 tctacttttt tcgaacacca ttggcagaac ttcttgttat ttcgtatttt tttgtgcgtc
     1801 agctaaagct tgatcgacat cttcgtttcc gacagttact tggttcactg cttttccgat
     1861 cacacctgag cttgtgatat ctcccatttt cgtgaaattc ttttccccga ctagtccaaa
     1921 gacttgtact tcatcaaaag cactagcaat ctcagatggc agctcgccaa aagacttgat
     1981 gacttcattt tctttgtaac catccagttc gactacttgt ttattcactg gttgagcacc
     2041 accaggagac atcaagaccc acttttccat gtttttcggt tgagataaga aagctgtgaa
     2101 tgcttcagca gcctcttttt gtttctcgtc tagaccagaa gaaatcgtca atccagatac
     2161 tgtgccatag acagcttgat ctttttgtgt tgggatagca aaaccgatat tttctgagtt
     2221 ccccgcttca taaacagaag gcaaaatgta agttgaatag attgccattg gtgctgttcc
     2281 attcatgaaa gcatctttga tttctgtcac atcattagag cctggcatcg tgtattggga
     2341 taagttttga taaaacgaca atgcttctct catcttttcc gtatcgatcg ttacctcgcc
     2401 ttcatcatcc aaaacatttg ctccatttga caaagcaaat tgagagaacg cttgctcact
     2461 cattgttcct tcagcagtag gcatagcaat tccatattgt ttattctctt tatctgtgaa
     2521 atgttgagca atttttaaaa tatcatccca attttctggt tctgaaaaac cagcttctga
     2581 taatttttct ttgtcatacc aaataccttg aacccagccg ctaattggtg cagcaatata
     2641 gctttttcca tcttctgaac gaacaaggtt tttggctccg tcataataat tatcttctcc
     2701 agcttcctcg ataacgtttt ggactgccgt ctgatcaatc agttcatctt tatccatcac
     2761 tttagcaaaa tcctgactga cttccattac ttctggtaat ttaccggaac gagctaacgt
     2821 aacgattttt gtattgaacg ctgattcttc taccggcact tgcttgattt tgatgtctgg
     2881 attttctttt tcaaaatcag caaccagttc attgatcaca cttaatcgtt cctgttcgac
     2941 agaagagtgc atgaattgaa tcgttgtctg ttgtgattcg ttagagcttt gactttcacc
     3001 acaagcagta agaagaaatc cagtagctag cagacaggta gtaacaataa tttttttcat
     3061 ttttttccac ctcttatttt attattttca gccacatata ttcatacggc tggaatatca
     3121 actttcgttc ttgactgcaa tggataattt gtccagaaaa caggtctagg taatctcctt
     3181 ttggtaaaag ataacctgct gattgattgg aaaaattatg gataactaag actttctctt
     3241 ccttcgcttc gcgaataaaa ctgataaaag aatctgctga gtccagtaag taaatcaaac
     3301 tattcggatg gaaggctttt tctttttttc gaatagatag catctgggtc aattcaccat
     3361 atactgctgt tcttaatgga tctgtctgca actctttctc aatatctgct aacaaaaatt
     3421 tttgccgatt gatttttcga ttctctcctg ttttttctac gcctttgtaa tcatttcgac
     3481 ttcccagcac actttgaata taaattgccg gcacgcctgg taacgttacc aaaaatgaat
     3541 gagcagctaa aaagcgtttg cttctccatt ccgtcgtatc tgttggagaa cttaacgcat
     3601 ctagatacgt agcgtttatc tcatatggac tggtcgttcc atctgggttt ttcttgtagt
     3661 ttactcgggc accctctttc ttcagctgtt ccactaattc aaggatcgtt ttttccggca
     3721 gaatacctcg taccggattc aatccgatac cgtcatggga ggctaaaaaa ttaaaaaaag
     3781 ttgttttttt cgatgcgaaa tcgatggttt ttaaccattc tttcaactta cttgtattac
     3841 ccgaacgaat cgcttgaagg gtcaaaggtg gtagcgaaaa ctgatagacc atctgtgctt
     3901 cattctcgcc attcccaaaa taactgatat tatctttgtg tggcacattt gtctcagtga
     3961 tcaataccgt tcctggagct gcttcatcag cgattgcacg gaataattgg atgattttat
     4021 gcgtttctgg caggtgtata caagaagttc ctagttcttt ccataaaaat cccacagcgt
     4081 ccaagcgaat aaatgaagcg cctttttcta agtaaaacaa aagaatatcg atcattttca
     4141 ataaaacttc tggattttta aaattcaagt ctacttgatc ttcgctaaaa gtcgtccata
     4201 cgtgtttttc tttcccgttt ttgtcaatga acgttgtaag taacgggctg gtccttggtc
     4261 tagtcacgct gcttgtatct gtatccagtg gcaaatcaat aaaaaaatct ttgtacgtat
     4321 cattcaagtt taaatattcc tgaaaccaag tactttcggc agacatatga ttgcagacaa
     4381 agtcgaacat taaccgtgtt tcgcttctca tttcttctat atcttcccat gtgcccaaac
     4441 ggtcgttgat tgcctgataa tttacaacgg agaaaccatc atcagatgta tatgggaaaa
     4501 atggcaagaa atgtacaact ctaaatgtat ctgccaaata cttttgataa aatttcttaa
     4561 atgtacgcaa agttggttgc tgctcttcaa aaaattgatc cccataagta atcaagaaaa
     4621 tatcttcttc gtcccaatgc agtttgcctg cctggccttt cttctgctga taaacactga
     4681 ctttttgttc aatcatagag gcggttttct cagacaaatc tgagtcgcga taaatcgctt
     4741 gtaaaagttt tgtatacatc ggattcccct ttcttttttg ggagcgttcc caacgatttg
     4801 atcatattat ggaaaacgct tactgtcaat agcatttttt taaataattt tgatatacac
     4861 aaaaatacaa ataagaaagc ttatgtatca ataattaata gcttcttaaa aaaatatggt
     4921 ctctcatgat catgcaagct attcctaaat tttcaaaatc atagacaaaa aagaatgtct
     4981 attgtactat ggaattattc taaaacgctc ccaaaaagga ggtgcatata aaattgaaac
     5041 ctactattta tgacattgcg aaaaaagcta atgtctcaaa atcaactgtc tccagagtat
     5101 taaataacca gccaaatatc tctgataaag caaaagcaag tgtacttcaa gcaatcaaag
     5161 aattgaatta tgaaccgagc aaaattgcaa gaggactgtc aggaaatggt tttgacgcga
     5221 ttctagcatt atctcatcgt acttctcata cgacctatgg caatcctttt ttttcagatg
     5281 ttattcaatc catttctgcc gtttctgaac aaaataactt cgacttgatt ctacaaacgg
     5341 caaaaacacc agaagaagag ctagaaaaat gcgcgaaaaa gatccaagaa aaaatgatcc
     5401 gaggaattat tattttaagc tctacgatgg atgaaacact tctttttgaa ttggatcaat
     5461 acaatattcc tattgtggta atcggaaaag tcggaaacga ctacacgaac atctactcag
     5521 tggacacgga taatttccag gatagttacg atttggtgga aacactgatc aagaatggac
     5581 accgtcatat cggctgtctc catgctccga cagaagttta tgtatccaat gatcgtctca
     5641 atggttaccg tcaatgctta tttgatcacc acctagatat ccgtaatgaa tggattatag
     5701 acggcggctt tcgcttagaa gatagcatgg cagcaatgaa tgatttaatg aagctacgag
     5761 aaagaccaac tgcgattttt tctaccgatg cattaaaatc attgagtatt tacaaagtgg
     5821 cagaacaatc tggaaagcag atacctgatg atctttcgat cgcaggattc agtgatccct
     5881 tattgtcctc cctattttcg ccacagttga ctcggataga cattccgaca aatcagttag
     5941 gaatcaaagc cacagaactt ttattccaac taatccacca ccaataccct gaagaaaaaa
     6001 gaatcattct ttcaaccgag attactctca cagattcgat ccaacaacaa gaaatgtaga
     6061 acagaattat aaaagaacag aaacactgag gataagtcag ttactctgtt cttttttcaa
     6121 tcactcatcc ctcgttaccc cctcccggta ttttagcgaa tttttcttat tttccatctg
     6181 ttttagatag ctgtggataa ctataatgtt tgtgttaaac tattttttcc acagggaaga
     6241 aaaaactatt tttaaccacg ttgtgtataa gtttttatag ttcgtttttt taaacacatt
     6301 tacccttatt tcaatccatg ttatttgggg atacttatct ctaaaacata aatagaataa
     6361 cagacatgtc tactggtttt gttttattcg aggaattatt caataatagc tagcaaaata
     6421 ttaaaaataa ccgctctatc ttcccatcaa tttattttgc attcaaaaaa tgatctatct
     6481 ttacttttgt tgattgactt ctcttggaag atgatcgttc cgatacctta attggatacg
     6541 aaccgatgtt gttaagatcc tttcccactt ttcttctagt tagacaaatt attttagaca
     6601 aaccaaaaaa gaggttgtga caataatttg tcacaacctc ttttttaaaa tatctatttt
     6661 gtttgattgt tcattgcgtc ttctgcagcc atttgcgctt caatatctga aatactgtag
     6721 acattttcgc ttgctacacc gacttctttt ggttccatgt cacgataacg ttgcatacca
     6781 gtaccagctg ggatgatctt acctataata acattttctt tcaaaccaag aagaggatct
     6841 ttcttaccgc ggatcgcagc atccgtcaag acacgagttg tttcttggaa tgatgcagca
     6901 gataagaaac tatttgtttc taatgatgct tttgtgatac ctagtaagac aggtcgagaa
     6961 gttgcaggaa cgcctcctga gactagcgta ttgtagtttt gatctttaaa gtctgcgatg
     7021 tctaataatg tacctggtaa gatatctgta tcacctggat ccattacacg aactttacgc
     7081 agcatttgac gaaccattac ttcgatatgc ttgtcgccga tttctacccc ttgcatacgg
     7141 taaacacgtt gtacttcacg aagcaagtag ttttcaacag ataatacatc gcgaacttgc
     7201 aacaattgtt ttggatcgat tgatccttct gtcaatggtg caccacggtg gatcatatcg
     7261 ccttctgcta ctttcatacg tgctgtataa ggaacagtat acgtacgtgt atctgttttt
     7321 cctttgattg ttacttcttt ggtacgtgtt gcaggatctt ctgagatatc gatgacatcc
     7381 cctgttactt ctgtgatcac tgcttgccct ttcggattac gtgcttcaaa gatttcttgg
     7441 acacgaggaa gaccttgagt gatatcgtct ccggcaaccc cacctgtatg gaatgtacgc
     7501 atcgtcaact gagtaccagg ttcaccgatt gattgagcgg cgatcgtacc aactgcttct
     7561 ccgacttcaa cgtcagagcc agttgcaagg ttgcgtccgt aacaatgttt gcagacacca
     7621 tgttttgtat tacatgtgaa gacagaacgg attgtcactg tttcgacacc tgcatcaacg
     7681 atttgttttg cgatgtcttc ggaaatcaat tggtttcctg caatgatcaa ttcatttgtt
     7741 tcaggatgac gtacttcttt acgggtgtaa cgtcccacta aacgatcttc taatgattcg
     7801 atgatttcat ttccctcgcg gatggcttgg atttcaagac ctcggtcagt accgcaatct
     7861 tcttcacgga tgattacatc ttgggccacg tcaaccagac gacgagtcaa gtaacctgaa
     7921 tcggcagtct tcaaggctgt atcggtcatc cctttacgag ccccgtgagt tgagataaac
     7981 atttccaaga cggatagtcc ttcacggaag ttcgaaatga ttggcaattc catgattcga
     8041 ccattaggag cggccatcaa accacgcatt ccggcaagct gtgtaaagtt ggaaatgtta
     8101 ccacgagctc cagaatcaga catcatgaag attggatttt tcgcttccaa accttccatc
     8161 agtttttgtt ggatgctgtc ttttgcagca ttccaaacag caatgacacg ttcataacgt
     8221 tcgtcatctg tgatcagacc acgacggaat tgttttgtaa tagtttcaac ttgtttatgg
     8281 gcattttcaa tgatttcttg tttttcattc agtacactga tgtcagcgat ccctactgtg
     8341 ataccggcat gtgtcgaatg tttgtaccct aagtctttca tacggtcaag catcttagaa
     8401 gtttctgtta ctttgaatcg tttgaagact tctgcgatga tgtttcctaa gtttttcttc
     8461 ttgaacggta atactaattc ttgctctttg atatgtgcag gaatatcagt accagcttca
     8521 acaaagtatt tgtccggtgt ttggacggtc aagttgaagt ctgttggttc attcagataa
     8581 gggaattcag gaggcatgat ctcgttgaag atgatcttac caactgttgt gatcatcgta
     8641 cgttcttttt gccattcagt aaatggtttt tcacctaatg agttagggtt cacaccgata
     8701 cgtgaatgta aatgaacata tccgttgcgc catgcgataa cggcttcgtt ttgatctctg
     8761 aagaccattc cttcgccttc gcgtccttct tcttccattg tcaagtagta gttacctaga
     8821 accatatctt gagaaggagt aacaactggt ttaccatctt tagggttcaa gatgttttga
     8881 gcagctaaca tcaacatacg tgcttctgct tgtgcttctt cattcaatgg aacgtggaca
     8941 gccatttggt ctccatcgaa atcggcatta taagcttcac atacaagtgg atgcaaacgg
     9001 attgcgcgtc cttgtactaa aactggttcg aatgcttgga taccaagtcg gtgaagcgta
     9061 ggtgcccggt tcagcaatac tgggtgttcc ttgattacgt cttctaaaac atcccatact
     9121 tcgtcttctt gacgttcgat tttacgtttc gcatttttga tgtttgaagc tagttcgcgt
     9181 tgaacaagct cacgcatcac gaatggtttg aataattcaa tggccatttc ttttggaagt
     9241 ccacattggt acatcttcaa gaaaggtcca acaacgataa cggaacgacc tgagtagtcg
     9301 acacgtttac ctagcaagtt ttgacggaaa cgaccttgtt tccctttcaa catatgagaa
     9361 agagatttca atgggcggtt acctggtcca gtaactggac gtccgcgacg accgttatcg
     9421 atcaatgcat ctactgcttc ttgcagcata cgtttttcat tttgaacgat gattccaggt
     9481 gcattcaagt ctaacaaacg tttcaaacgg ttattacggt tgattacacg acggtaaaga
     9541 tcattcaagt cacttgttgc aaaacgtcca ccttctaatt gaaccattgg gcgtaaatct
     9601 ggtggaataa cagggataac gtccataacc atccagcttg gttgatttcc tgaagcgcgg
     9661 aatgcttcta aaatatccaa acggcggatt gcacgtgtac gtttttgtcc ttgtgcagat
     9721 ttcaattctt ctttcaattc ggctgcttct gcatctagat caacattatc aagtaattgt
     9781 ttaatcgctt ctgcacccat agcagcttgg aattcttgtc catattgttc acgtttttcg
     9841 cgatattcgc gttctgtcaa caattgtttt ttctctaaat tcgtatttcc tggttcgatg
     9901 actacatatg aagcaaaata aatgatttct tccaaagcgc gtgggctcat gtctaataca
     9961 agacccatac gtgaaggaat ccctttaaag taccagatat gtgaaacagg agctgctaat
    10021 tcaatatgtc ccatgcgttc acggcgaact ttcgaacgag taacttccac tccgcatcga
    10081 tcacaaacga ttcctttata acggatacgt ttgtattttc cgcaggcaca ttcccagtct
    10141 tttgtaggac caaaaatccg ctcacagaac aacccttcgc gttcaggttt taaggtacga
    10201 tagttaatcg tctcaggttt ttttacttca ccgtaagacc agcttctgat tttttcggga
    10261 gaagccaaac ctatttgcat actttcgaat ttatttacat cgatcaaaag gggttccctc
    10321 catttcattt aatggcaaac cgcaagacgg ctgatcttcg accgccttgc agctcataga
    10381 ccggattagt ctaaattatc ttctgcggtt tcaaagtttt ggatgacatc gtctttttca
    10441 tcttccactt gttcagcagc tttcttttct aattctttgg cagtttgctg ttcggcaaat
    10501 tttgtcaaag catcaacagt gatcaaatcg tcgtcttcat cgtccatgtc gcgaagttcg
    10561 atctctgttt cttcgatatc caagacacgc atgtccagac ctagtgattg caattctttc
    10621 accaatacgc ggaatgattc cggtacacct ggttttggaa ttggttcacc cttgacgatt
    10681 gcttcgtatg ttttcacacg tccgactacg tcatctgatt tgtatgtcaa gatttcttgt
    10741 aaagtataag ccgcaccgta agcttccagt gcccatactt ccatttcacc aaaacgttgt
    10801 ccaccaaatt gagctttacc tcccaatggt tgttgtgtaa ccaatgagta aggtccgatt
    10861 gaacgtgcat gcaatttgtc atcgaccatg tgagcaagtt tgatcatgta catcacaccg
    10921 acagacacac gtccatcaaa tggttcacct gtacgtccgt cgtacagaac agttttcgca
    10981 tcgctggcca taccggcttc acgaactgtt tcccatacgt cttcatcact tgcaccatca
    11041 aatactggcg ttgctacgtg gatacctaac tgacgtgcag ccatacccaa gtgtaattcc
    11101 aatacttgcc cgatgttcat acgtgaaggc acccctaaag gattcagcat gatatcgatt
    11161 ggtgttccat caggtaggaa tggcatatct tcttccggca taatacggga tacaacccct
    11221 ttatttccgt gacgaccggc cattttatcc ccttcatgga ttttacgttt ttgaacgata
    11281 taaacacgaa ctaacatgtt tacgcctggt gacaattcat ctccagcttc acgagtaaag
    11341 attttcacat cgtgaacgat accgccgccg ccatgtggta cacgtaatga tgtatcgcgg
    11401 acttcacgag ctttttcgcc aaagatcgca tgcaatagac gttcttctgc agataattct
    11461 gttacccctt ttggcgtgac tttccctaca agcaaatcgc catcttggac ttctgcacca
    11521 atacggatga tacccatttc gtctaaatct tttaatgcgt cttccccgac attagggatt
    11581 tcgcgtgtga tttcttcagg tcctaatttt gtatcgcggg cttctgattc atattcttca
    11641 atatggatag aagtgtatac gtcatctttt accaaacgac ggctcatgat gatcgcatct
    11701 tcatagttgt acccttccca agtcatgaaa ccaactaaaa cgttttggcc tagagccatt
    11761 tcgccttgtt ccatagatgg tccgtctgcc agagtatccc ctttttcgac tttctcaccc
    11821 aaatgaacga ttggacgttg gttgtagctt gttccggagt ttgaacgacg gaattttgtt
    11881 acatcgtatt tatctaatgc accgttgtcg cggcgtacgc ggatttcaga agcatcaacg
    11941 tattcaacga caccgtcatg tttacataac aaagcagctc ctgagtcatg cgctgatttg
    12001 tattccatac cagtaccaac ccatggtgct tgaggattga tcaacggcac tgcttgacgc
    12061 tgcatgttgg cacccatcaa ggcacggttg gagtcatcgt tttccaagaa aggaatacat
    12121 gctgtcgcaa cggcaactac ttgtttaggt gatacgtcca tgtagtctac tttatcaata
    12181 gatacttcta agttttcact ttgagcacga gccataacaa cgtcttgagc aaatgttcca
    12241 tcttcattca atgggctgtt tgcttgggct acgatgtaat ggtcttcgat atcagctgtc
    12301 aagtagtcga tttggtcagt aacacgacct gtttcacggt ctacacgacg ataaggtgtt
    12361 tcgataaatc cgaatttatt tactttcgca taactggaca aactattgat caacccgatg
    12421 tttgggcctt caggagtttc gatcggacac atacggccat agtgggagta gtgaacgtca
    12481 cgaacttcat aaccggcgcg gtcacgagtc aatccgccag gtcctaaggc tgaaagacga
    12541 cgtttgtgtg tcaattcgcc aagagggttt gtttgatcca tgaactgtga caactgagaa
    12601 gaaccaaaga attctttgat acttgccaca actggacgga tattgatcaa ttgttgaggt
    12661 gtcaatgttt cagtatcttg gatcgacatt ctttcacgaa ccacacgttc catacgtgct
    12721 agcccgatac ggaattggtt ttgtaaaagt tcaccgactg aacggatacg acgatttcct
    12781 aagtggtcga tatcatcgac attgccgata ccttccatca agttcaagaa atagctcatt
    12841 gaagcaatga tgtcggctgg acgaaccgtt tttacagctg cttctggata gccgtttccg
    12901 atcacgttta cttcacgttc agggtctctt ggtgaaaata ctttgatgac ttggacagtc
    12961 attggatctg tgaccacacc gtcttctgat ggatagtaag tcactgaatt taggccatta
    13021 tcaatgaaag gagctagtgt ttccatgact tggtgagtca gaactgttcc tttttcaacg
    13081 atgatctcgc ctgtttctgg atcaactaat gtttcagcta atgtcagatt caatagacgt
    13141 gttttcaaat ctaatttttt gtttactttg taacgaccaa cgtttgctaa gtcgtaacgt
    13201 tttggatcga agaaacgagc attcagcaag ttacgtgagc tgtctgctgt ttttggttcg
    13261 cctggacgca aacgttcgta aacgtctttc aaaccttctt ctgttcttga gtcgcttgcg
    13321 tttttatgca agtctttttc gactgtattg cgtaatgttt cgctgtcgcc gaagatttca
    13381 aagatcgtat catcggaacc aaatcctaac gcacgaacta atacggttaa aggaatctta
    13441 cgtgtacggt cgatacgtac ataagagatg tcttttgcat cagtttccat ttctagccaa
    13501 gctccacggt tagggataac agtagaaccg aatccttctt ttccgttttt gtcgactttt
    13561 ccatggaaat agacacctgg tgaacggact aactgagaaa cgattactcg ctctgccccg
    13621 ttgatgatga atgttccttg ttctgtcatt aatgggaaat caccgaagaa tacttcttgt
    13681 gctttgattt cgccagtctc gcggtttgtc aaacgcaatg ttacatgtaa tggtgcagag
    13741 tagtttgcat catgcgcacg agcttctgct actgtatact ttggttcttt caattcataa
    13801 tctacaaatt ccaaagatag attgccattg aagtcatcga ttggcaaaat gtcttcaaac
    13861 atttctctaa gtccctcatc taagaaccat tggtaagagt ctgtttgaat ttcgatcaaa
    13921 tttggtaatt ccaacacttc actgattctt gcgaaacttc tacgttcgcg atgttttccg
    13981 tattttacta cgtgtccagc caagctcttc acccctctaa aatgtcattc aaaaagacta
    14041 cccgatcaat tgttacaata atatttcaaa tattattttt aggtaacaac agacattttt
    14101 tgcggctttt aggcaaaaaa aaaccaaaaa tagaacattc tcttgaaacc ttacttcgtg
    14161 agcttctact tttgtttcct tatattaaag ccgaaacgga caatatttcg tttctgcctt
    14221 ttcttcatca gatagttttt gcaacttcat atactacact tattttctat aaaagtcaac
    14281 acttcctggc gtttcttctg ctttttcgca acattttctt aatgttttca attttatgtt
    14341 ttcaattatc tgacaattct taattgacag acctttttat ctcggttata ctttagaaaa
    14401 ttcattttgt ggaggaactt ctatgcgaac caacatcaac caaatcgcac caacacaact
    14461 gaataacttt tactttagct actttagata ggtttgtatt catcatgatg gatacaaacg
    14521 ttaaacgaca gtttgtatct atgatggcta aagaactttg tattgcagca aaaagccacg
    14581 tagacaaaat gctaagtggc tgagcagtga aaatacaaag taaaagtatc aagcattcta
    14641 ctttttcaat ttattttccg acttttcaat ccatttggac attttgtctg aatggatttt
    14701 ttgtttccca aaattaagga ggaaaaagaa atgaaaaaaa atataatcat cggattaggc
    14761 gctgccctgc tactagcttt aggaggttgc gcacaaaaag acaattccgc agaggcatca
    14821 gatggacaaa aaacaattgg cgtattgcaa cttgtacaac ataactcgtt agatgctgcc
    14881 tataaaggat tcaaagaagg attagcagaa gaagggtata aagaaggcga taatctaaca
    14941 atcgattatc aaaatgccca aaacaatcaa gacaacctga aaagtatgag tgagaaactc
    15001 gtcaaagaag atcctgattt gctacttgga atcgccactc cagcagcaca atctttatta
    15061 aatgaaacaa ccactatccc gatgactgtc acagcggtca cagacttgga acaagcccat
    15121 ttagtcacat ccaatgaaaa acctggcggc aatgtgactg gcacgacaga tatggtgcca
    15181 atcgaaaaac aaatcggctt actgttaaat atcgtgccag acgccaaaac aatcggcatc
    15241 atgtataaca acggtgaagc aaactctaag atccaagctg atttggcaga aaaagaattg
    15301 aaaaaagcgg gcgtcaaagt caaagtactg acagcaaatt ccacaaatga tgtccaacaa
    15361 gtgacgaaaa gtctagctaa agatgtagat ggaatctata ttcctactga taacacgttc
    15421 gcaagtgcgg caagtgttgt cggtgaagtc gcaaaagaaa caaaaacacc tgtcgttgct
    15481 ggcgcagttg atcaagtcaa agacggcggt ctagcaacag taggaatcga ctacgaaagc
    15541 ttgggcaaac aaacagggat catggcagca aaaatcttag acggcaagaa tccaagtgaa
    15601 cttcctgtcg aaacagcgaa tgatttggaa ctgtatgtca ataaagacat ggccaaagca
    15661 ttaggcatca atccagacag catcaaagaa gctgaataaa acacaggagg attctcatga
    15721 caaatttgct ctctcttttc ttatcatctg catcgcaagg ggtcttatgg gccctacttg
    15781 cgattggtgt atacgtcact ttccgtatct tggacattgc cgacttgact gctgaaggaa
    15841 gctttccttt aggtgcaggg atcgcggcag taagtattac aaatggctat catccattga
    15901 cagcttgtct tctaggcttt ttagggggtg cggcagcagg gcttgtttcc ggactgcttc
    15961 acacaaaatt gaaaattccc gcccttcttg cagggatcat caccatgact ggtttatatt
    16021 ctgtcacatc ccgtgtgatg ggtgcgccga acatctcttt attgggtcag ccaacgatct
    16081 ttcattgggt cgagtcattc ggcataacaa aagacaacgc tgtactgatc gtcggtctgc
    16141 tttttgcttt gatcgttgta tgtttacttg ttttgttctt acgtacagaa acaggacttg
    16201 ctattcgtgc aactggcgat aatctggcaa tgagcgaagc aaacgggatc aacacagata
    16261 atatgaagat catcggttat atgatttcta atggatgcat tgctctttct ggctccttac
    16321 ttgcgcaaaa caacggcttt gctgatttaa attctggtat tggaacgatc gtaattggac
    16381 ttgcttctat tatcatcgca gaagttttgt tacgtaacca gccactattt ctccgtctct
    16441 tgactgtcgt tttaggtgcg gtcatttatc gttttatttt agcgttagtc ttcgaactga
    16501 atgttgaacc aagtgattcg aaacttgctt ctgctttcgt gctagtgatc tgtctttctt
    16561 tcccgcagat ccagcaaaaa ttccatttac ctcaaaaaaa gaaaggcggt agtgtcaaat
    16621 gaatgacgtc ttaacattgg aaaatctcca tcataccttt gaaaaaggaa cgatcaacga
    16681 aaatcatgtc ctgaaaggca ttaatctttc cttagatccc ggcgacttcg tgacgatcat
    16741 cggtggaaat ggggctggta aatctacttt gctgaacagc atcgccggta cattttctgt
    16801 cgatcagggt caaattttat tgaatggaaa aaatatcacg aaaaaatctg ttgtcgaacg
    16861 ttcgaaatcc atcagtcgcg tattccaaga tcctaaacta gggacagccg ttcgtttaac
    16921 ggtagaagaa aatcttgctc ttgctatgaa acgaggaaaa aaacgcggct tcttccgtgg
    16981 tgtcaaacca caagaccgaa catttttcaa agaccagctg gttcggctaa atttggggtt
    17041 ggaaaatcgt ttatcctcag aaatcggttt gctgtctgga ggacaacgtc aagctattac
    17101 gttattgatg gccacattac agcgtccaga actgatctta ttagatgaac acactgcagc
    17161 gcttgaccct gctacttcca aaacggtgat ggaattgaca gaaaagctga tcactgagca
    17221 gaatctcact gcttttatgg tcactcatga catggaagac gcgatccgct atgggagtcg
    17281 actgatcatg ctgcatcaag gaaggatcgt cgtagatatc cagcaagagg aaaaacagca
    17341 gctcacagtg ccccaattat tagcgttgtt caaacaaaac agcggcacgg cattaaaaga
    17401 cgatcaagta cttttatctt cataatcaga aagaggatgg gacaaaaaat gaacgcccac
    17461 cctcttcttt tttctctttc cttctttcgt ttttgcttta tgtatcaacc gttgtttctt
    17521 cgaaaactat tttgttctct ccttcttcct gatagaatgg atgggaaagg agcttgatgc
    17581 catgacaacg aaagacaagg tacttcaatt attgaaaaag acaccagatt ttctttccgg
    17641 agaaaagttg gcacagcaac tggaagtttc ccggacaagt atttggaaag ccatcaaaga
    17701 attggaaaag gcaggctatc gttttgagca cgggcctaca gggtatcgtt acctgccctc
    17761 tgacgtacta gatgcaaaag aaatccagca agagctacgg tcgtttattc ctgatttgaa
    17821 agtacaaacg atgttgactt ctgaatctac gatgaaagat gccaaactgg cagcagctca
    17881 aggagattct gtttcgaagt tatttgttgc tgatacacaa gaacaagctc gtggacgttt
    17941 tggacggcct tttttcgctg aacctggacg aggaatctac atgagtctct tgctccatcc
    18001 agacaaacgg tttgatgaat tgcctcaata tacgatcatc gcagccagtg cctgtgtgaa
    18061 agccattgaa tctgtaaccg gtcgttctgc ttcgatcaaa tgggtcaatg atatctacat
    18121 ggatggaaaa aaagtctgtg ggattttatc tgaagcagtc agtgatatgg aaagcggcag
    18181 gatcagtcat atcatcattg ggatcgggct taatttttct gttcctcaaa atcattttcc
    18241 agctgatctc caagaaaaag ccatctctgt ctttcccgac ggacaagcta gtgtcacaag
    18301 gaatcagcta atcagcaaaa tctggcaaga attgttcact tatctttctc acccagaaaa
    18361 agcgttggac ttctataaag aaaaatcatt cgtattggga aaaaccgtgt cgttcaatca
    18421 aaaaggaacg acttatgtcg gtcttgcaaa agaaatcggt gatcatggcg aactgatcgt
    18481 ccaaacaaat gaacagaaga tgagtttgtc ttctggcgaa atcagtctca catctatccg
    18541 tacgcaataa ttatcagatt tcacaaaagg acacctgatc tcatacagtg tccttttttc
    18601 tgaattctct tcaaaagact ctccttattc tatcagctga cagtttctct atcctctaga
    18661 aaaaggtatt ctaaaaatat ctataaagca tggtacactt tctttaaatg aatcaatgca
    18721 aaaaacaaga gagtcatcag tttacacgat caaaagagat agcaggccgc ttcctttttc
    18781 aatcttatag atagaccatg ggtaacgata aaaacaaaaa agaacttgcg acttattttg
    18841 ccgtaagttc ttttttagct agtgccttaa tctcttaatc ccaacaaacg tctcgcatag
    18901 atcacctgac cagagtgcat agcagcatca tcaataatag aaacgagacg cattcctcgt
    18961 gtgactggtg gtgtccagtt ctcgtcaatt acttcatcta gctcggaagg ttccagcctt
    19021 cttaagtaag aaatagcaaa atcagcagct cctcgcaagt atcctagcag atcttctatt
    19081 tgattagctt ggatctgctt tgcttgttcc agactatgct tccaatcttg ttcgttatct
    19141 gctatcgtca tcggaaaccg gctttcccag tcttgactat gccaaattgg agaagttcct
    19201 gctaaatcag caatctggaa atctaattct cgagcggtat gccaagccag ccaagtcatt
    19261 gatttgatct gcggggctct atcttccact ggaaaacgat tggcctcctc aatagagacg
    19321 ccttcaaaca aacgttcaaa ccgatcgtat gcccgatcca cactctcgca tagcatatct
    19381 aaataatcca tttattttcc tcctttttat ttatcctact tactttttcc taattctagg
    19441 atacctactt atatgttact acacaccctt attttttaca ctttaacagt caccatcatc
    19501 aatagtttgg agattgaaag aaaaagatag tgtcgaacta tcaattgaac aagaagcaac
    19561 aatatgaacg tttcattaat attctttaaa taaacaatga tttttcaaac gaacagttta
    19621 agactaggag aaaaagagta taataggaaa atgtttgaaa ggaaaggaag gggaaaaatg
    19681 aaaaaaaggg caagtttact agtcctagtt gctttgttgt tcaatatctt ttcgtatggt
    19741 gtggccgttt cagcggaaac atacattcct aaagaccctg ggaatattta taatgatcta
    19801 actggtaact atggcttgtt aggaattgct agtcaattcc acgtatttgc taaaaataag
    19861 acaactgtga atgcacatac agacggaaat atagcaacac atgaacttga tgcaaaaaat
    19921 aattttggta cagagcttca tgaaggttta ttgaaccaag aaataaatta tatacaaaaa
    19981 actgattctt tcgctaattc ttcaggcata cctacaggta gcacaagaat gaataaattt
    20041 gtcgttggca gtaacgtgga tgtagcactt caagatggtc atcctacagt caatggatcc
    20101 aggcttgatc acttaactcc agatgatttt tataaagatc gtgctgggca tgaatatatt
    20161 gatttcgagc aggaatttgc taaattgaat gtagcagcaa atgatcttgc aacgatcact
    20221 ccagctaaaa catatactgc tgcagatttt ccagacatga acaatcggac aatcgattta
    20281 actgatttat cagataccgg gtttttattg gtaaatatcg atgcagaagt gttaacgatg
    20341 aatacgccac ttcaaatcat taatccaaat gatcaagtag ttgtatttaa tgtcatcaac
    20401 agttcttctg cattgaacgt acagtcacca atcaaataca atgatcgttc aaatcatgaa
    20461 acagaagatt tttcggatgc gaatatctct tggaattttg gaaatgagat gactgatttg
    20521 acaatcagtg ctccttttca aggaacaatc cttgcgccta atgctactat ccgcattact
    20581 caaaaccaag acggaacgat tataggaaca aatgtgattt tagatgcagc taccaatcgt
    20641 tgggatccta acgaaatatt tatcagcgac aatggtacag atacgacaga cacaagcgac
    20701 agtacagata catctaccac aagtgatagc tcagattcat ca
//
LOCUS       DACBZN010000042        20372 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.042, whole genome shotgun sequence.
ACCESSION   DACBZN010000042 DACBZN010000000
VERSION     DACBZN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.042"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..189
                     /locus_tag="IVD64_001506"
     CDS             <1..189
                     /locus_tag="IVD64_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6148764.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IVD64_001507"
     CDS             complement(1055..1585)
                     /locus_tag="IVD64_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP6148765.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IVD64_001508"
     CDS             complement(1582..2199)
                     /locus_tag="IVD64_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6148766.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2805..3002)
                     /locus_tag="IVD64_001509"
     CDS             complement(2805..3002)
                     /locus_tag="IVD64_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP6148767.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYK"
     gene            complement(3180..4712)
                     /locus_tag="IVD64_001510"
     CDS             complement(3180..4712)
                     /locus_tag="IVD64_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6148768.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IVD64_001511"
     CDS             complement(4848..5933)
                     /locus_tag="IVD64_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6148769.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IVD64_001512"
     CDS             complement(5948..6667)
                     /locus_tag="IVD64_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6148770.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IVD64_001513"
     CDS             complement(6664..7974)
                     /locus_tag="IVD64_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6148771.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IVD64_001514"
     CDS             complement(8001..8591)
                     /locus_tag="IVD64_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP6148772.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IVD64_001515"
     CDS             complement(8909..9970)
                     /locus_tag="IVD64_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6148773.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IVD64_001516"
     CDS             complement(10538..11605)
                     /locus_tag="IVD64_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6148774.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IVD64_001517"
     CDS             complement(11625..12104)
                     /locus_tag="IVD64_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP6148775.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IVD64_001518"
     CDS             complement(12121..12582)
                     /locus_tag="IVD64_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6148776.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IVD64_001519"
     CDS             complement(12596..13846)
                     /locus_tag="IVD64_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP6148777.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IVD64_001520"
     CDS             complement(14224..15675)
                     /locus_tag="IVD64_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6148778.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IVD64_001521"
     CDS             complement(15996..16760)
                     /locus_tag="IVD64_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP6148779.1"
                     /translation="MIDLHCHILPGIDDGAESLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKLNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IVD64_001522"
     CDS             complement(16788..17486)
                     /locus_tag="IVD64_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP6148780.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IVD64_001523"
     CDS             complement(17498..18277)
                     /locus_tag="IVD64_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP6148781.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGFVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IVD64_001524"
     CDS             complement(18293..19237)
                     /locus_tag="IVD64_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6148782.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IVD64_001525"
     CDS             complement(19283..20062)
                     /locus_tag="IVD64_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148783.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         6485 a         4050 c         2621 g         7216 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatctattt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttaagtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg ttaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagctt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac aacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccgaatcgt acgatattgt
    17401 tcagcgattg gtgaagaggg attggtgagt gtgaccaaac tgactgcatg agtttgggct
    17461 gctttttgtt tctgtgttcg tgccattttc tttcctccta gactcttgag cggcttctgc
    17521 gtgatggcgc tgttttatcg tttgtagctt ttggttgtgt ctgagctggt ccttttttac
    17581 ggataccagc attcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgaatc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagccc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatcacgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctcagcaa
    19321 cagtttgtgc ttttgttgcg tatgctgcgc ttattttttc attccggatc tgctctattt
    19381 gactgagcac cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttta
    19441 acatcgtatc gaataactga tctagttgcg tgacttgcgc gatctgaata tcagcgaatc
    19501 ctagatattg cataacaacc gttttccaag gactatcttt aaaaaagctc atattttcac
    19561 gtacacgagc gacatctgca cttgtcacct tttctttgat gatgacatcg tctacagctt
    19621 gctgccagtt tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt
    19681 ttgattctgc actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag
    19741 cttctttagg caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg
    19801 atagcttatt tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaaattt
    19861 tttcttttgt actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt
    19921 gattggtgat ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga
    19981 tatggtatcc aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg
    20041 acttgacgaa tggcttttcc atagattcac tcctaagtat tgatgataga taaattaaga
    20101 tataattcac ttacataaaa atgacagctt tttatttaaa aaaacataat taaactaata
    20161 tattcacaga aatatttgta aacaagtttt ccctatataa tcataccaca aactgttttc
    20221 ttgactaggg caatttacga tttaaaagta aataaaacat taataagata aaaattaatc
    20281 gatttcattt ttatttccat aattttcata ggtgttttta aaagttacac aaaaaataga
    20341 ataacaaaaa aattaacatt gaattaataa ta
//
LOCUS       DACBZN010000043        20368 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.043, whole genome shotgun sequence.
ACCESSION   DACBZN010000043 DACBZN010000000
VERSION     DACBZN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.043"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            295..1404
                     /locus_tag="IVD64_001526"
     CDS             295..1404
                     /locus_tag="IVD64_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6148784.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1487..2383
                     /locus_tag="IVD64_001527"
                     /pseudo
     CDS             1487..2383
                     /locus_tag="IVD64_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2399..3223
                     /locus_tag="IVD64_001528"
     CDS             2399..3223
                     /locus_tag="IVD64_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6148785.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3255..4565
                     /locus_tag="IVD64_001529"
     CDS             3255..4565
                     /locus_tag="IVD64_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP6148786.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4713..5972
                     /locus_tag="IVD64_001530"
     CDS             4713..5972
                     /locus_tag="IVD64_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6148787.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5982..6854
                     /locus_tag="IVD64_001531"
     CDS             5982..6854
                     /locus_tag="IVD64_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148788.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6866..7696
                     /locus_tag="IVD64_001532"
     CDS             6866..7696
                     /locus_tag="IVD64_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6148789.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7736..9919
                     /locus_tag="IVD64_001533"
     CDS             7736..9919
                     /locus_tag="IVD64_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP6148790.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9980..11200
                     /locus_tag="IVD64_001534"
     CDS             9980..11200
                     /locus_tag="IVD64_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP6148791.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11197..12657
                     /gene="gtfA"
                     /locus_tag="IVD64_001535"
     CDS             11197..12657
                     /gene="gtfA"
                     /locus_tag="IVD64_001535"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP6148792.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12734..13840
                     /locus_tag="IVD64_001536"
                     /pseudo
     CDS             12734..13840
                     /locus_tag="IVD64_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14162..14371
                     /locus_tag="IVD64_001537"
     CDS             14162..14371
                     /locus_tag="IVD64_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6148793.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14364..14513
                     /locus_tag="IVD64_001538"
     CDS             14364..14513
                     /locus_tag="IVD64_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP6148794.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14548..15332
                     /locus_tag="IVD64_001539"
                     /pseudo
     CDS             14548..15332
                     /locus_tag="IVD64_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15616..16701)
                     /gene="xerS"
                     /locus_tag="IVD64_001540"
     CDS             complement(15616..16701)
                     /gene="xerS"
                     /locus_tag="IVD64_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP6148795.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            17035..17268
                     /locus_tag="IVD64_001541"
     CDS             17035..17268
                     /locus_tag="IVD64_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP6148796.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            17265..17672
                     /locus_tag="IVD64_001542"
     CDS             17265..17672
                     /locus_tag="IVD64_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP6148797.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(18809..19327)
                     /locus_tag="IVD64_001543"
     CDS             complement(18809..19327)
                     /locus_tag="IVD64_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP6148798.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         6902 a         3141 c         3790 g         6535 t
ORIGIN      
        1 aaaaagtaaa catccaaaaa agggtgttta cttttttgtt tactaatatt tttagtaaaa
       61 aatctcgaca aaacattaag taaacagtta tggtgttgtt aacaaaaaga aaacgcattc
      121 aaaacaggag gaattaagat gaaacatttc aaaaaaatag ctttaacagg gatagctgtt
      181 ttaactggga cattactcgc agcatgtggt gggggagata gtaattctaa agacgggcaa
      241 gtaactttaa gctatgcgag ttgagatact gaccaagctc caggactaag aaaaatgtta
      301 gatgaatttg aaaaacaaaa tcctgatatc aaagtagaaa tggagacaac gccttgggat
      361 caatattggg ttaaattaga agctgcaaca acgggaggta atatgccaga tgtagtaacc
      421 atgcactcta gcgaatccta taaatatatg tcacagaatg ctttgatggg acttgatgat
      481 gttatcaaag aaaatgatat tgacatggga aactttactg aaggaattgc tgatttctat
      541 acgcttgaag acgaactata cgcaatccct aaagatgctt ctgtagtggg gctatggtac
      601 aacaaagaat tatttgataa tgcaggtgtc gattatccag atgaaacatg gacatgggaa
      661 acactcagac aagctgcaat tgatttaaca gatgaatcaa aaggcatcta tggttttgca
      721 tcacatattg gaacagagac tggctactgg ccatttattt atcaaaatgg gggagaagta
      781 tttgccgatg acaatacaaa atcaggtttg aataccccag aagcacaaga agctttagct
      841 ttttatcgag atattgcact tgttgatcat gcttcaccaa caattacaga attacaagat
      901 cctgataagt taagtcgttt tcaatcaggt agattagcaa tgatgattga aggaaactgg
      961 tttgcaccgg gatttttgaa aaatgactat ctagttgaaa atgttgcttc tgccgtgtta
     1021 cctaagggaa aagttaatgc aacaattaca aatggacttg gttgggctgc cagtgcgagt
     1081 acaaaatacc cagaagaagt gaaaaaacta atggcatttt tagctagtga ggacgccaat
     1141 aaaatccaag cggaaacggg ggcatcaatc ccagcactaa aaggctttga agacgagtgg
     1201 gctgcttctt cagatacaat tgatttgagt gcttttggaa aaatgttgga gtatggagtc
     1261 cctcgtccat acaataaaga tggattacga gctgaacaag cagaagttca aattattaac
     1321 cgtattctgt ctggtgaagt aactgttgaa gatggaaccg ctcaaattga aaaagaagtc
     1381 aatgcgatat tgagcggaaa ttaaattagt taattataca atatacaggc gaaaggattc
     1441 tatttcgtct gtatattaat ggatgaatac caaaaagggg gatattatga aaaatcgaat
     1501 ttttggaaaa tatcgtaaag atcaagtgat atggggctat atttttattt ttccaacggc
     1561 attaggatta attttattaa atatatggcc agcaattcaa acagcaattt taagttttca
     1621 aaaaacggta gggttcggtg atactgaatg ggtcggatta agaaattacc aaaaaatgtt
     1681 tgcagacggt gaagtttttc agtctttatt aaacacatta atttatgcag tggtttcgat
     1741 acctagtatt gttgtattat ccttgttggt ggcagtttta atgaatagaa aagttaaagg
     1801 attgtcgatt tatcggacaa tttattttct accagtcgtt gctgcaccat ccgcagtcgc
     1861 aatgatttgg cgctggatgt tcaataatga ttatggactg attaataaca tgttggctaa
     1921 aataggtctc gatggaccag catggttaac ggatccatcg attgcgattt attctattat
     1981 cattgtagga gtttggagtg ctattggtta taacatggtg ttattactag ccggcttata
     2041 agaaattcct aaagattatt atgaagcagc ggaaattgat ggtgctagtc caatccagca
     2101 gtttttcaac attacgttac cattagttac accaacatta ttttttgtag ttgtaacaac
     2161 agttatcaat gctttccaag tatttgatgt tatttttatg atgattactc ctaatagtac
     2221 cgccatgttt aaatcacaat cattagccta tttattctat aaacattcat ttattttaaa
     2281 tgacaaggga tatggttcgg caatcgttat gttcctttta gtgattatat taattattac
     2341 tgcaattcaa atgaagttgc agaaaaagtg ggtaaactat taaggaggct atcaaattat
     2401 gaaaaataaa tttttttcaa atgcgccaat ccatatcatg cttattttag gtggtattgc
     2461 aatggcatta ccttttattt ggatgatttt aacttcaggg aaaacgttgg ttgaatctac
     2521 ccaaattcca cctacaattt tccctgagaa atttcagcta agtaactatc aacaagtact
     2581 tgatttatta ccattcatgg ctttttatat caatacattt ttaatggttg ttggacgagt
     2641 gattggctcg gttttatttt cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc
     2701 agggaaaaat atccttttca gcttagtact tattcaaatg atgattcccg gttccatttt
     2761 tattactcca cagtatttat tagcacaaaa attgggtatt ttaaatacaa ttccagcatt
     2821 aatattacca ggggtagtta gtgcgtttgg tacgttttta ttaagacaat ttttcttagg
     2881 tttacctgat gaattggagg aagcggccta tttagaaggt gccaatattt ggacgatctt
     2941 tttcaaaatt atgttgccgc ttgctaaatc aggattggtt gcacttggaa tattcacagc
     3001 gttgtttgca tttaaagatt taatgtggcc attgattgtc aatacttcaa ttgataagat
     3061 gcctttagct ccgggattag caaacttaca aggacaatat gccacgaact tcccacaatt
     3121 aatggcaggt tcgttattag caatttggcc aatagttgtg atattcatcg ttttccaaag
     3181 aaaattcatt gaaggaattg ctacaacagg tagtaaatta taaaactatt aattagtgat
     3241 tggagagtaa gtacatgaca aaaattacat ttattggtgc aggaagtact atttttgcaa
     3301 aaaacgtatt aggcgatgcg atgttaactc caagtctaca agatgcacat attgcattgt
     3361 acgatattga tgaaaaacga ctaaaagaat caaatttaat gcttaaaaat attaataaaa
     3421 attcgaatga aaatcgtgct gatattgttt cttattatga tcgcaaagaa gcgttaagag
     3481 aggctgattt tgttatcaat gctattcaag tgggtggtta taaaccttca acagtgattg
     3541 attttgatat tcctaaaaaa tatgggttgc gacaaactat tggtgacacc atggggattg
     3601 gtgggatttt tagaacccta cggacactac cagtaatgtt agactttgct aaagaaatgg
     3661 aagaagtttg tccaaatgct tggttattga actatacaaa tccgatggcg atgttaacca
     3721 tgggtgtttt aaaagcgacg agtattaaga ctgttggttt atgtcatagc gttcaagtat
     3781 gcgtaccaga attgtttgag catttaggaa tagccgatca atatgactta aatgactttc
     3841 aatggaaaat tgcaggcatt aatcatatgg cattcttgtt ggaaattaca aaaaatgggg
     3901 aagatttcta tccaacaatt cgtcagttag ctgcagaaaa accaaatcca catccagatt
     3961 ctgtacgttt tgaattgttg aaacgttttg gttactatat tacagaatca agtgaacaca
     4021 atgcagaata ccatccatac tttattaaat caaaataccc agaattgatt gacgaattag
     4081 ggattccgtt ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca
     4141 tgcgagagga cattgtaaat aatggggagt taacacatac gagaagccgt gaatatgctt
     4201 catatatcat ggattctatt acgacaggaa ctccgtcagt gattgcagga aatgtcttaa
     4261 ataaaggctt aatcacaaac ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg
     4321 ataaaaatgg cgtgcaacca acctatgtcg gtgaattgcc aacacaatta gctgctttaa
     4381 atcggacgaa tatcaatgtt caagaactga cagtagaagc cgctttaaca ctagataaat
     4441 caaaaattta tcaagcagcg tatttagatc cacatctatc atctgaattg tctttaacag
     4501 atatcaaaaa tttagtggat gatttaatcg aagcacatgg ggactattta cctgaatata
     4561 attaaaaaga agctgaaaga gtaaggaatt ttcaattcct tactcttttc ttagttaaat
     4621 ttagtaaaaa tattgacaaa acacttagta aacagttaca ttagtgttaa caaaaagaaa
     4681 acgcattcaa attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag
     4741 cttgtatagc actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg
     4801 caagcggtaa ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc
     4861 aggagattgc taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg
     4921 taccgaatgc tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca
     4981 ttaatcttta tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg
     5041 acttaaccga agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta
     5101 ttgaggatcg tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca
     5161 aaactgcttt tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa
     5221 tagtagctga gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct
     5281 ggactttaaa tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag
     5341 cgaacaatgt ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc
     5401 aaaaagattt taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc
     5461 aaggtgccgg ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc
     5521 cgaatggttc atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa
     5581 cttttccttt tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag
     5641 ccttatcagt ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata
     5701 tgacaacacc tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg
     5761 agggcgttat cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta
     5821 ctgatcgtca tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct
     5881 taacaacaaa ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt
     5941 tctttaatcc aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt
     6001 taaatcgtta ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt
     6061 tctactttcc aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt
     6121 ataattttga ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta
     6181 tcaaatcgat tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt
     6241 ttggtatttt gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag
     6301 catggttctt ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa
     6361 tttttaacta tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca
     6421 gtcttttagg aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag
     6481 gaattgccat gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta
     6541 ttgaagctgc tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca
     6601 cctacttatt accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag
     6661 catttgatca aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg
     6721 ggttacttgt ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg
     6781 ctttagtctt atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa
     6841 aatttgaaat ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata
     6901 cttgctttta ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg
     6961 ttcatttaaa acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac
     7021 tttggaaaat ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc
     7081 aatcatcaca atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc
     7141 actagcaaga aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt
     7201 aggaattttt gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat
     7261 tggtttagca aatatgtggg gattaatctt actctatctc acttatgccg taccacagac
     7321 actattttta tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc
     7381 tgaaattgat ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa
     7441 accaatgcat gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct
     7501 accattatta attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa
     7561 ttatacggga caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat
     7621 tgtcacgatt acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa
     7681 tggagcagtg aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat
     7741 tatatttgac aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt
     7801 aggaatcgaa gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta
     7861 ttctggtggg tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc
     7921 gcaagatcgt ttattatctt tagacacatt accattagaa tttccaggat atggacatgg
     7981 tgattttaga gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt
     8041 tcgttatgat agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt
     8101 atatacggaa gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag
     8161 tggattgact ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc
     8221 tacgcaatta gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc
     8281 aattgatttt ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga
     8341 acgtcaaatg actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg
     8401 atcaagcagc catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt
     8461 tcaaggagag gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca
     8521 aaaagatcca tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg
     8581 gcaactaaaa actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga
     8641 aggtctgaat acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg
     8701 aaactatcgt ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga
     8761 ttttaacgaa gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat
     8821 gtttgtttta gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga
     8881 ttgggttgaa acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca
     8941 tgataacggg atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga
     9001 actatttcga gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag
     9061 tcgaaatcaa tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca
     9121 aatgacggcg atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa
     9181 tatgacagaa gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg
     9241 ttatatgtta gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt
     9301 atttgaaagt tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc
     9361 acaaacttgg acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac
     9421 cagcttagtg tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca
     9481 aacaggaaga gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg
     9541 ttatgagcta gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt
     9601 tgccttttat aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag
     9661 tccgtttgaa ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat
     9721 ggtcttttat ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc
     9781 aggattagca gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcgatgaatt
     9841 gatgaatctc ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt
     9901 ttatttgaaa cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact
     9961 cattccaaac gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt
    10021 gaattgggaa aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga
    10081 taaaggacta ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta
    10141 tggcatcttt gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa
    10201 tcagtcgggc attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa
    10261 tagtatgacg gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga
    10321 agatggctat tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac
    10381 tggtgaatgg tttaaagata gccaatggac atggacggat atgcgtacca atatttttac
    10441 gaatcaatta gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca
    10501 accacgttca attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat
    10561 gttggcgacg ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact
    10621 aggaatgacc aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc
    10681 tcagtataaa cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa
    10741 taaacgaagt cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag
    10801 attctcaact tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc
    10861 agaagctcag ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt
    10921 aagaaaaaat agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga
    10981 taaaaatgaa catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat
    11041 cttaaacgtt acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt
    11101 aatcaataat tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt
    11161 tgaaagtatt gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg
    11221 ctcatcacct atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac
    11281 aaacatttac aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt
    11341 gatcgaggct ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa
    11401 gatgtggaag cattaggtga agattattat ttgatgtttg attttatgat taatcacatt
    11461 tctcgtgaat caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa
    11521 gaaatgttta tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt
    11581 gatttaatct acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt
    11641 acgacagaag aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa
    11701 gaagttgtga aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct
    11761 ttaattcgat tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc
    11821 gtggaaccag atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac
    11881 gtcgaattat taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat
    11941 gattattata tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga
    12001 aaatcagaac gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg
    12061 gatacgcatg atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg
    12121 gattacacct ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt
    12181 gccaattaca ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc
    12241 gataatgacc gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca
    12301 caaatttatt acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg
    12361 aaagaagggc gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg
    12421 caacggccag tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg
    12481 tttgatttgg atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca
    12541 cgtaaaaata aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa
    12601 tttatcattg agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct
    12661 attccataga acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa
    12721 aaaggactca tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc
    12781 cataaaatta ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta
    12841 tcgtaaaaaa ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga
    12901 aaaagaaagt ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac
    12961 cgatgtgtca tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa
    13021 agtgttagaa gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac
    13081 caagcgaaat catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca
    13141 taagtgggcg actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta
    13201 tatggacgat actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga
    13261 tctcaatcaa ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg
    13321 gtattaggac tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg
    13381 gattaaaaaa aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga
    13441 aattctacga gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg
    13501 actagtcact gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga
    13561 cgtctcttca aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt
    13621 caagcacatc aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt
    13681 gcagaatcaa ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga
    13741 gacgaaaacg gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca
    13801 gccctttgaa tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg
    13861 ttacttttgg ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg
    13921 cgtcagcaca gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa
    13981 tgttttatgc aacactagtg atatttttta gaattagatt tctaatgtgc attttttgag
    14041 tattaaggag caagtataca aagatttata aagctgatta aatttgaata aaaatatgca
    14101 ggaacgctac tatttttagt agtgttcttt tttatatttg aataaaaaat gtggtggtta
    14161 aatggtggtt ataacaaaaa atgaattaat gaaattaggt tatgggaagt atcagtctga
    14221 gagaatcata cgacaggcaa aagcaattat ggtttcaaaa agctatacct attacgaaaa
    14281 tagaagatta ggactagtac ctgttgaatc tgtagaagaa gttttaggta tgaagataga
    14341 ggtagaggag gattttcaga atcatggtta attttaaaaa agataaaaat gggaaatatc
    14401 attttactgc tagtttggga ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag
    14461 aggggttaaa acagttaaag aagaaaaaaa gcgtataccg aacttgtttc taattatgga
    14521 aaagaagcag aaacttctca aagtactatg ttgtttgagg agttttttta taaattattc
    14581 ctaccttatt atcaagggaa agtagaagaa agaacatata aaaatcggtt atctacaatc
    14641 aaaaaaactt ttcttatttc tttaaaatgg agttaagaga aatacgtcca attgacattc
    14701 aaaagtggca aaataattta ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg
    14761 ggatttttca gatgtcatta gatcgagcag ttgttttggg actacttgaa aaaaatcctg
    14821 caaaagttat tggaaatgtg aagaaaagga aaactacagt tgatttttgg acaaaagaag
    14881 aatttacaaa agttatttca actttttata ttgatgattt gtaccaacat tttggtttta
    14941 tttgtatgtg gttacttttt atgaccgtta tgcgtttggg agaagccact gcattaactt
    15001 ggaaagatat tgattttgaa aataaaaact tgcgtgtgaa taaaacactc tattataaga
    15061 gagccaaaac ctatgagttt gtagaacccg aaacggcttc tagtattcgt acaatcgaac
    15121 tagacgacaa tacaattacc tttttaaaag cttggaagga gcgtcaagtt tcaataggag
    15181 gcatgtcgtt tgtactttct tataatggaa cacctactca gaaatataca atccgtcata
    15241 ttatgagaag acatgcaacg atcgttggtg ttcatataat ccgcatacac ggattaaggc
    15301 atagtcatgc ttctttgctt atatcacaat aaaaagatgc acttcgaatt aaagaaaggt
    15361 taaaacatgc tgatgtacaa acgactttag gaacttatgg tcatttgtat ctaaattcat
    15421 tgagagagat aacaaatgat cccacagatt atatccaaac taatttttct aaaatcaaac
    15481 cgacacctat ttacgataat aaaaaataaa tgataaacaa aagtaggatc acaccaaaag
    15541 aaacacacta gaaaaactat aatacaggct tttctagcgt gttgtttctt atttccgcct
    15601 atttatacat tatactcata aagcatttaa tgcatcgatc gtagccgcat tgtctatatg
    15661 ggtatataaa gttgttgtat tggtacttga atgtcccaat tgttgggcag ttaataccaa
    15721 tgaatttgtc tgttggtaga gtctagtagc taatgtatga cgcagtttgt gagggctgac
    15781 ttgaattcta aagccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
    15841 acgcttggct tctccttggt aaattgctag aaataaagct ttttcgtttt ctgttacttt
    15901 atatcgttct ttccgaatag ctagataatt agctaaatat tccattgcga atgctgctat
    15961 attgacagca tctcttttat ttccttttct agttactacc acattattct tatctaaaga
    16021 taggtcatct aaattaatat ttataacctc ggagagtctc aatccagatc ctaaaatcag
    16081 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttact
    16141 caaagtattt ttatattcat tggcaatata atctagataa ttttgtgtat ccttatctag
    16201 aaataatttt ttctcaatgg cattggcacg tgcagctaac gtagttttat ctgtgatggt
    16261 tgctacttga tccataggat tattgtctaa gtatggtttt cctagaggat tattggaact
    16321 tttcgaaaga tatttaaata gaacctttaa tgccgtgata tttctattga tagtagaagt
    16381 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
    16441 ttcttttgtg cgacgtttca aatgagattt gtacaagttt aggtcatagg cagttaactg
    16501 tcttatcgag gtgatagaag tatcaggaac gatagactca ctcagaagcc attcataaaa
    16561 gagcttataa ttttttagat attctaaagt ggttcgaggt gattgatctt tatcctcacg
    16621 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
    16681 atatatttct gtatttttca tgtcattcat cctttcaata agtaggggag aagagtatct
    16741 ctataattgg actataccaa ttatagaatg ttttgtcaat tggtatgctc taaaatttat
    16801 taatttgttt caaaaaaagc atataaacag gtatacgttt aataatttaa aataaatggt
    16861 atacataaat agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt
    16921 aattatatag aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt
    16981 taaaatcaac aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca
    17041 attaaagaaa gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag
    17101 tttcttaaag aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt
    17161 cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa
    17221 gcattaactg aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc
    17281 tagaagccaa agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg
    17341 aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct
    17401 cagcttttgg tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac
    17461 aattgataaa gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt
    17521 ttgtcttttt gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt
    17581 tagcggtata tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca
    17641 acactataaa gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt
    17701 tattagctgc tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa
    17761 ctatatagaa aacgtaaatt ttctatatag ttggattaag ataacgacta tttattttat
    17821 catattttta ctatacccta tacttttact tggtatattt atgaataatc gcaaatttac
    17881 ccctatacta ctagtttgta agttagatta ataaaaatac taaagttatt tgttctttta
    17941 ttaaatacac ctacaactta taaattgatg gtatttagat aacacgaaag aataaaaata
    18001 acagctgata gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat
    18061 gaacttgctt ctgaaaactt atgaaatgaa agacctttta catcttgcag aaatcggtac
    18121 tgggattaaa ctaagcaata aaaactaatg ttcccctcat tctttgaact tctactattt
    18181 gatttattta ttctgataaa tgacgatttc accttcagga tcttcaaatc ccgtatagaa
    18241 tttatttaag atctcttcag tatcagtaat aggataggcg ataggccaca taccctttga
    18301 tggttagaat aaactgctca aaatctgtat cgtatgttgg ctttagcata ttaacaaagg
    18361 ctatctccaa attataaacc ataagcgttg aatcgttctt aacaatttca attcttccta
    18421 acccaaagtc agaagatata attaccggac ttgccttacc cttcatgtgg gttgttgaga
    18481 ttccatattt aaagggaatt gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa
    18541 aaaaatcttt cccctgctag tctcctaagt ccctttgtag cggatatggc accataatct
    18601 cattttatcg aagtgagtca tggtttatct gttggccaac caaaattgat acaatctaac
    18661 gatgcaccct ctacggcatt ctaggggagt gcacttgcat tcacccaata aaaaccggca
    18721 gttagcaaag aacttatgta gtcgtatgtt cttactgtta acatacaaga ggtctccttt
    18781 ccacaattgc ttagcagtaa acgggaattt acaaagctaa tctcttccat tttcttatct
    18841 ttgttctaaa agtcccaaat ggagcaactg tatttacatg aataaattta tatacttccc
    18901 atacagccgt tttggttgcc tcgtctgccc attttctttt gtgtgcagtg aatagatctt
    18961 catcactcat tgaatcaatc atcatgttaa tatcatcaat attacgattg agaatttcct
    19021 ctaattcttt caatggtaga tgtgcatagg tatttgtaaa ccacttatat aactctccta
    19081 actgattcca tttaaattga tcagaaggcg ttttaacaga taatccattt ctttcatcat
    19141 tttcccattt tagaattaaa ttcgtccaac caacttgata tgctaaattt tcagcaggtg
    19201 ttctatccac ttcctcaact cttttatcct tcaattcttc aggaatagtt tcaaattcta
    19261 aaatatattt agtataagtc ctatttattt ctttttttaa ttcttcttta tttttatatg
    19321 ttctcattat attacctcca taaattccta attgtctcct ttaattcatc tataatttca
    19381 tccacgatat tttaatccgt tctacaatgc atatctgata tgcattgact gtctaactca
    19441 tatgtgaatg atttataaaa tatcccaacg tctcatctaa agaaatgttg atcgtcagca
    19501 aggagctaaa aaatacgaat atatttcttt tgtagtaaaa tcggactggt ttgcatatca
    19561 ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa ttactaataa aaactaccac
    19621 tttaagcgtt aagaactgtt aagtttagtt aagtgaggat agttcacaaa atccattgtt
    19681 aaacttcacg aaagtggtaa aactcaaaat gctttatata ggattacagc atcgctcttt
    19741 cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa attgacgatg acacgttcct
    19801 tacggctcat cagatgaaac agctacaaaa acgaaatgat caactcgaag aaaatttaat
    19861 tctaaaaaag cgattgccat attcacgcct cgctcgacga actattaaag gtcaagattt
    19921 acctgaagat catccttttt aggaaattga agctttttat gttgcacaat gcatttataa
    19981 tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga aacagataca
    20041 cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg ttaatcttcc
    20101 gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta aggttggcaa
    20161 aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt tggttgacac
    20221 ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt acctaaaaag
    20281 tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat ttcaacttat
    20341 atgttttagt gaactacccc cacttagc
//
LOCUS       DACBZN010000044        20181 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.044, whole genome shotgun sequence.
ACCESSION   DACBZN010000044 DACBZN010000000
VERSION     DACBZN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.044"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="IVD64_001544"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="IVD64_001544"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP6148799.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="IVD64_001545"
     CDS             1011..2258
                     /locus_tag="IVD64_001545"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP6148800.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="IVD64_001546"
     CDS             2334..2678
                     /locus_tag="IVD64_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148801.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="IVD64_001547"
     CDS             complement(2795..3121)
                     /locus_tag="IVD64_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148802.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="IVD64_001548"
     CDS             complement(3494..4696)
                     /locus_tag="IVD64_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6148803.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="IVD64_001549"
     CDS             4813..5328
                     /locus_tag="IVD64_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6148804.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="IVD64_001550"
     CDS             complement(5527..5973)
                     /locus_tag="IVD64_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6148805.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr"
                     /locus_tag="IVD64_001551"
     CDS             complement(6020..7498)
                     /gene="msr"
                     /locus_tag="IVD64_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6148806.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="IVD64_001552"
     CDS             complement(7852..8481)
                     /locus_tag="IVD64_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6148807.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="IVD64_001553"
     CDS             complement(8465..8929)
                     /locus_tag="IVD64_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6148808.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="IVD64_001554"
     CDS             complement(9416..10684)
                     /locus_tag="IVD64_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6148809.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="IVD64_001555"
     CDS             complement(10915..11652)
                     /locus_tag="IVD64_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6148810.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="IVD64_001556"
     CDS             complement(11719..12288)
                     /locus_tag="IVD64_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6148811.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="IVD64_001557"
     CDS             12416..13288
                     /locus_tag="IVD64_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6148812.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="IVD64_001558"
     CDS             13630..14502
                     /locus_tag="IVD64_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6148813.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /locus_tag="IVD64_001559"
     CDS             14499..16457
                     /locus_tag="IVD64_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6148814.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="IVD64_001560"
     CDS             complement(16708..17559)
                     /locus_tag="IVD64_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6148815.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="IVD64_001561"
     CDS             17682..18149
                     /locus_tag="IVD64_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6148816.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="IVD64_001562"
     CDS             complement(18412..18894)
                     /locus_tag="IVD64_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6148817.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="IVD64_001563"
     CDS             complement(18994..19857)
                     /locus_tag="IVD64_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6148818.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6066 a         4155 c         3664 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgatg aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tacttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DACBZN010000045        19992 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.045, whole genome shotgun sequence.
ACCESSION   DACBZN010000045 DACBZN010000000
VERSION     DACBZN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.045"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            1..813
                     /locus_tag="IVD64_001564"
     CDS             1..813
                     /locus_tag="IVD64_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP6148819.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            810..1610
                     /locus_tag="IVD64_001565"
     CDS             810..1610
                     /locus_tag="IVD64_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP6148820.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1771..3039
                     /gene="rseP"
                     /locus_tag="IVD64_001566"
     CDS             1771..3039
                     /gene="rseP"
                     /locus_tag="IVD64_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP6148821.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3107..4816
                     /locus_tag="IVD64_001567"
     CDS             3107..4816
                     /locus_tag="IVD64_001567"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP6148822.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5028..9380
                     /locus_tag="IVD64_001568"
     CDS             5028..9380
                     /locus_tag="IVD64_001568"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP6148823.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9524..9997
                     /gene="rimP"
                     /locus_tag="IVD64_001569"
     CDS             9524..9997
                     /gene="rimP"
                     /locus_tag="IVD64_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP6148824.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10021..11196
                     /gene="nusA"
                     /locus_tag="IVD64_001570"
     CDS             10021..11196
                     /gene="nusA"
                     /locus_tag="IVD64_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP6148825.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11218..11511
                     /locus_tag="IVD64_001571"
     CDS             11218..11511
                     /locus_tag="IVD64_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP6148826.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11508..11819
                     /locus_tag="IVD64_001572"
     CDS             11508..11819
                     /locus_tag="IVD64_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP6148827.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11832..14138
                     /gene="infB"
                     /locus_tag="IVD64_001573"
     CDS             11832..14138
                     /gene="infB"
                     /locus_tag="IVD64_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP6148828.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14165..14512
                     /gene="rbfA"
                     /locus_tag="IVD64_001574"
     CDS             14165..14512
                     /gene="rbfA"
                     /locus_tag="IVD64_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP6148829.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14620..14841)
                     /locus_tag="IVD64_001575"
     CDS             complement(14620..14841)
                     /locus_tag="IVD64_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6148830.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15289..17652
                     /locus_tag="IVD64_001576"
     CDS             15289..17652
                     /locus_tag="IVD64_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP6148831.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18020..18943
                     /gene="truB"
                     /locus_tag="IVD64_001577"
     CDS             18020..18943
                     /gene="truB"
                     /locus_tag="IVD64_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP6148832.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18947..19888
                     /gene="ribF"
                     /locus_tag="IVD64_001578"
     CDS             18947..19888
                     /gene="ribF"
                     /locus_tag="IVD64_001578"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP6148833.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6860 a         3512 c         4388 g         5232 t
ORIGIN      
        1 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
       61 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
      121 gcgcaaaacc gtcgtttgcc acgagttgct ggccataaag aaggcatgga aacagtaaag
      181 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
      241 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
      301 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
      361 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
      421 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
      481 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
      541 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
      601 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
      661 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
      721 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
      781 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
      841 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
      901 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
      961 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     1021 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     1081 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     1141 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     1201 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     1261 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     1321 cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat
     1381 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     1441 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     1501 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     1561 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     1621 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     1681 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     1741 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     1801 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     1861 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     1921 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     1981 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     2041 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     2101 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     2161 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     2221 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     2281 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     2341 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     2401 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     2461 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     2521 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     2581 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     2641 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     2701 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     2761 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     2821 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     2881 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     2941 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     3001 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     3061 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     3121 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     3181 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     3241 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     3301 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     3361 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     3421 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     3481 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     3541 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     3601 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     3661 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     3721 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     3781 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     3841 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     3901 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     3961 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     4021 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     4081 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     4141 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     4201 ccactataaa aatgtgaatc cagaacgcga tttcactgtg ctttcttatg aagatcttcg
     4261 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     4321 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     4381 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     4441 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     4501 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     4561 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     4621 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     4681 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     4741 aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt
     4801 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     4861 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     4921 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     4981 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     5041 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     5101 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     5161 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     5221 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     5281 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     5341 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     5401 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     5461 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     5521 tggatgagca gcaagcggaa cgtgtattga aactttttga agaacgcaaa caagaacagg
     5581 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     5641 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca gctaggacga aatattccag
     5701 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
     5761 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
     5821 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
     5881 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
     5941 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
     6001 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
     6061 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggaa
     6121 aatggggaca ccgagctatc gccattacag accatggtgg tgcgcaagcc tttccggaag
     6181 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
     6241 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
     6301 acgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
     6361 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
     6421 caggacatcc attgtcacga acaacggtag atctgactgg tatcacggat gaaatggttc
     6481 gagggtcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
     6541 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
     6601 atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt
     6661 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaagtttggt gttagcttgg
     6721 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
     6781 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
     6841 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
     6901 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
     6961 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
     7021 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
     7081 gaaaccgagc aaaattctat gattatattg aagttatgcc aaaagcggtc tatgcgcctt
     7141 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
     7201 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
     7261 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
     7321 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
     7381 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
     7441 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
     7501 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
     7561 cgttacctga gatcattcaa aaacgattag aaaaagagtt gaattctatt aatggtaatg
     7621 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
     7681 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
     7741 ctgaagttaa tccattagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
     7801 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
     7861 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
     7921 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
     7981 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
     8041 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
     8101 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
     8161 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
     8221 cgatccaata tccagcagac gatcaaaatt cagaatggaa aacgactcat tttgatttcc
     8281 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
     8341 ttcggatgct tcaagattta tcagggatcg acccacaaac gatccctacg gatgacccgg
     8401 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
     8461 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgttag
     8521 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
     8581 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
     8641 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
     8701 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
     8761 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
     8821 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
     8881 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
     8941 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
     9001 aacaagcaat gaaagaaatc acggacaagg gattagatgc ttccgtaaaa gaaaagaatc
     9061 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
     9121 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
     9181 catttagagc tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa
     9241 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
     9301 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
     9361 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
     9421 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
     9481 ttttaatgga attacgcact taatttttaa ggaggcgaaa cttttgagta gcgtcgttga
     9541 aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac ttgtcgaagt
     9601 cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca aagaaggcgg
     9661 tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc
     9721 agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg gagcagagcg
     9781 gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc atgtctcctt
     9841 ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat ttgatgctga
     9901 acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa
     9961 aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa tcagaaaaaa
    10021 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    10081 gaaatcgtga tcgatgcatt agaagcagca ttggtttctg catacaaacg tcactatggc
    10141 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    10201 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    10261 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    10321 gattttggac ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    10381 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    10441 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    10501 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    10561 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    10621 ccagacctat tgcgtcgctt atttgaacaa gaagtaccag aagtctatga tggaatcgtt
    10681 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    10741 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    10801 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    10861 gtattcatcg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    10921 aattcaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    10981 cgcggacaaa atgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    11041 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    11101 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    11161 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    11221 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    11281 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    11341 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    11401 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    11461 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    11521 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    11581 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    11641 ccgatgcaag tgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    11701 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    11761 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    11821 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    11881 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    11941 cgattactag cagtgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    12001 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    12061 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    12121 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    12181 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    12241 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    12301 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    12361 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    12421 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    12481 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    12541 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    12601 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagta gtaacgatta
    12661 tgggacacgt agaccacggg aaaacaacct tattggatac attgcgccat tcacgtgtaa
    12721 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    12781 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    12841 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    12901 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    12961 taaataaaat tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt
    13021 atgaattaat tcctgaagca tggggtggag aaacgatttt tgttgagatc tcagcgaaat
    13081 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    13141 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    13201 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa
    13261 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    13321 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    13381 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    13441 gcgcaaaacg tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact
    13501 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    13561 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    13621 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    13681 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    13741 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    13801 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    13861 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    13921 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    13981 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    14041 tgggttatga atgtggtgcg atgattgaaa aattcaatga catcaaagta gatgacgtaa
    14101 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    14161 cattatggct aactaccgtg atcgccgagt cggtcaagaa atcttaaaag aagtcaatga
    14221 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    14281 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    14341 agataagaaa aaagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    14401 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    14461 tgtctatggg aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaaa
    14521 tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt
    14581 acctaagagt tccatgactt tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag
    14641 aaaagtaaaa aattcgactt tcttttttct atagtatata aatttttgca tatagataaa
    14701 gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag tcaggtatag
    14761 ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg tcggtccgtt
    14821 gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc cgcttcgatt
    14881 tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc tgggatacca
    14941 attcacatat tgacaaagat aacagagact tttacataaa acggatcaat caggaaagga
    15001 attgctcgat ccagccccag ttttccaaca atgtgttaac tggcctagcg tcattcagca
    15061 aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata acgacaagtt
    15121 ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt
    15181 tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag gtttcaattg
    15241 ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat ggattattct
    15301 agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta tctttctgtt
    15361 gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc agaagatgtc
    15421 aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat ttatgtccat
    15481 ttgaaccgag tcatcaataa atatgatttg aacatgtttt atatcgaagg gccaggacac
    15541 ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga gatctatcca
    15601 gaagtaacgg aagatgagac aggaatgcaa aaattattca agcgattttc atttcctgga
    15661 ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg cggagagctt
    15721 ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt aatctctgct
    15781 gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg gttctcaaat
    15841 gtctttatca atccagtgat agatggagct gttttaccga tccttcattt aaatggtgca
    15901 aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc aaattacttt
    15961 aacggacttg gctgggagcc tttctttatt gaaggaaatg atccagaaaa attaaatcct
    16021 gttatggctg aaaaaatgga tcaagcaatc gagaaaatca aatccatcca aaaagaagct
    16081 cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca
    16141 ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg aaccttccga
    16201 gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga tgctttactc
    16261 cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg tattttagaa
    16321 gaaatcagag aaattgcgcc tactggtgac caaagaatgg caaaaaatcc tatcaccaat
    16381 ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac tttgcaattt
    16441 gaaaaactag gctccgttaa agcagaagat atgaccgaac ttggtaaatt cgttcgcgag
    16501 atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac aaaatcaaac
    16561 cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa
    16621 aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa
    16681 gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt tgcaagttac
    16741 gaatcattct tgcgagtggt tgactctatg ttgactcagc acttcaaatg gatacggaaa
    16801 tcacatgacc tttcttggcg aaatgattat ccttcgctaa atctcattgc aagctcaaca
    16861 gtcttccagc aagatcacaa tggatatagc caccaagatc caggaatatt gacacatttg
    16921 gcagaaaaga aggcggagtt catccgtgaa tatctgcctg cggatgcaaa tacattgctc
    16981 gcagtcatgg ataaagcatt ccactcgtca gaaaaaatca atttgatcat ctcttccaaa
    17041 catccgcgtg ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg
    17101 aaaatcattg attgggcttc gacagcaaaa gaggaggaac ctgaattggt cattgctgct
    17161 gcaggaacgg aatctaactt agaagcatta gcagctgcaa ctttgctgct ggaagaattc
    17221 ccaaaactaa aaattcggtt cattaatgtc gtagatttat tgaaattacg tcatccaagt
    17281 caagatccgc gtgggttgag cgatgaagaa tttgatcaat attttacaaa agacaaaccg
    17341 atcttatttg ctttccacgg ttatgaaaca ttggtccgca cgattttctt tgatcgtcat
    17401 aaccatcatt taatgattca tggctacaaa gaaaatggag atatcacgac accatttgac
    17461 atgcgtgtag taaatgaact tgatcgttat catttggcaa aagacgcagc tttaaaaatc
    17521 aaaggaagtc aagcagaaga ttttgcggaa aaaatggatc agaaattaca agaacatcaa
    17581 aactatattc gtgaaaatgg tattgatctg ccagaagtat tggattggaa atggaaaaat
    17641 cttgatcaat aaaatgataa aatcgatccc gcaaaacaga attttttaca ttgattaaga
    17701 aactgaaaaa ataaataaaa aaaacggaaa aaagactgga agaatggaca gtcttttttt
    17761 tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa atccaatcaa
    17821 atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaacat aacatgattt
    17881 ctatttacgt gagcatgaat tttgttacgt ttttattgcc gaaaaataaa aaccgttcat
    17941 tatgatgtca aattcgtcag taattgatgc aatgtgttat aataattcag ttaaatcaaa
    18001 aatgaaatgg agaaaagaca tggacgggtt attaccttta tggaaagaac gcggaatgac
    18061 aagtcatgat tgcgtattca aattaagaaa aatcttacat acgaaaaaaa tcggacatgg
    18121 gggtacgctt gacccagacg tagacggtgt gttgcctatt tgtattggaa aagccacaaa
    18181 agttatcgag tatttgacag attctgggaa aatctatgta ggcgagataa ctctaggttt
    18241 cagtacaacg acggaagatc gatccggaga acgggtagaa acaaaaccag tagatatgtc
    18301 tttatctgaa gaaaaaatag atgaagtgat ggcttcattt attggtgaga tcacccagat
    18361 accaccaatg tactcagcgg taaaagtcaa cggtaagcgg ttatatgaat atgcccgcaa
    18421 taatgaaaca gtggagcgtc ctgttagaaa ggctcagatt taccgtttta tccggacaag
    18481 tgaattgaca agagacgaag tgacaggaac catttcttgg cgcttcgaag tagaatgcgg
    18541 gaaaggaacc tatgtccgca cattagctgt agatacaggc aagaaacttg gttaccctgc
    18601 gcacatgtct gatctgaccc gaacagcaag tgccggtatg tcccagaaag atgctctgac
    18661 attagaagaa gtagctcaag ctgtgaaaaa tgggacaatc gatgattgtt tgctgccaat
    18721 agaaaaagga gtgaaacgat tcaaacgagt agatattact gatgaagtat ggcagaaagt
    18781 aaaaaacggt atgagacttg attatactgt atttgcgctc ccagaaatgc cgtcagaaga
    18841 aattgctttg ttttataaag gcaaagttgt cagtatctat cagccaaacc cgaaagaaaa
    18901 aaacaaatta aaacctagta aagtattgag aaatgaggaa taacgaatga atatcatcaa
    18961 gatcagacat ccttaccaat cagaacaaat tccagaagac gatgtcgttt tagtactggg
    19021 attttttgac ggcgtgcata ttggacacca gaaagtcata aagacaggta tggaaattgc
    19081 acaaaaagaa ggattgaaat tagcattgat gactttcaat cagcatccat cgattgtctt
    19141 taaaaagatc gatcctagca gcgtgaaata tttgactagc cttaagcaaa aagaagaaaa
    19201 aatggccgct ttgggtatag attatttata tgaagttgat tttacttctt cttttgctca
    19261 tttagcaccg caggaatttg ttgatcaata catcgtaggg ttacatgcaa aatatgctgt
    19321 ttccggtttt gattatacgt atggaccaaa agacattgca gatgtcgctc attttcctga
    19381 atatgccaaa ggaagattca aaatcgtcac agtttctaaa gaagaagaac aaggtgaaaa
    19441 aatcagctct acccgtattc gaggattact agacagcgga caagtaaaag aagcagcaaa
    19501 actattaggt tgcacatatg aggtagaagg ggtagttgtc catggcgatg cacgaggcag
    19561 actactagga ttccctacag caaatgtcaa agtaaagagc acagttcatt tgcctaaaga
    19621 aggtgtttat gtatcggaaa taaaaattgg ggaaacttgg tatccagcca tgggttcaat
    19681 tggacataat gatacattcg gtgaaggacg ggagctgact gtagaattgt atattttgga
    19741 ctttaatcaa gatatctatg gtgaacatgt cgcagtacgc tggaatcaat ttttaagaga
    19801 tcaagtgaaa ttttctagtg tagaggaatt gattgaacaa ctcaaaaagg atgaacaagc
    19861 aacagctgaa tatttttctc agcaataaaa cattatgcat aaaatctctt gtttatgtat
    19921 aactagcaat ttcaataaaa tgggtgataa ataagcccag gaaaggggct gtgacaaaag
    19981 tcttgtcaca gc
//
LOCUS       DACBZN010000046        19450 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.046, whole genome shotgun sequence.
ACCESSION   DACBZN010000046 DACBZN010000000
VERSION     DACBZN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.046"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            126..3653
                     /locus_tag="IVD64_001579"
     CDS             126..3653
                     /locus_tag="IVD64_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6148834.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IVD64_001580"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IVD64_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP6148835.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IVD64_001581"
     CDS             7441..7782
                     /locus_tag="IVD64_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6148836.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IVD64_001582"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IVD64_001582"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP6148837.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IVD64_001583"
     CDS             9139..11559
                     /locus_tag="IVD64_001583"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP6148838.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVD64_001584"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVD64_001584"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP6148839.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="IVD64_001585"
     CDS             12993..13730
                     /locus_tag="IVD64_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6148840.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="IVD64_001586"
     CDS             13744..14559
                     /locus_tag="IVD64_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6148841.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="IVD64_001587"
     CDS             14572..15219
                     /locus_tag="IVD64_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6148842.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="IVD64_001588"
     CDS             15235..15891
                     /locus_tag="IVD64_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6148843.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="IVD64_001589"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="IVD64_001589"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP6148844.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="IVD64_001590"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="IVD64_001590"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP6148845.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="IVD64_001591"
     CDS             18233..18751
                     /locus_tag="IVD64_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP6148846.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="IVD64_001592"
     CDS             18858..19358
                     /locus_tag="IVD64_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6148847.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3340 c         4177 g         5392 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc
//
LOCUS       DACBZN010000047        19186 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.047, whole genome shotgun sequence.
ACCESSION   DACBZN010000047 DACBZN010000000
VERSION     DACBZN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.047"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(38..1189)
                     /locus_tag="IVD64_001593"
     CDS             complement(38..1189)
                     /locus_tag="IVD64_001593"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP6148848.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IVD64_001594"
     CDS             complement(1202..1639)
                     /locus_tag="IVD64_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6148849.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IVD64_001595"
     CDS             complement(1654..3420)
                     /locus_tag="IVD64_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP6148850.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IVD64_001596"
     CDS             3684..4361
                     /locus_tag="IVD64_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148851.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IVD64_001597"
     CDS             4364..5491
                     /locus_tag="IVD64_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148852.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IVD64_001598"
     CDS             complement(5691..6131)
                     /locus_tag="IVD64_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6148853.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IVD64_001599"
     CDS             complement(6142..8175)
                     /locus_tag="IVD64_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6148854.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGLFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IVD64_001600"
     CDS             complement(8177..9880)
                     /locus_tag="IVD64_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6148855.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IVD64_001601"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IVD64_001601"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP6148856.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IVD64_001602"
     CDS             complement(10963..11988)
                     /locus_tag="IVD64_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6148857.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IVD64_001603"
     CDS             complement(12197..13066)
                     /locus_tag="IVD64_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP6148858.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IVD64_001604"
     CDS             complement(13525..15417)
                     /locus_tag="IVD64_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148859.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IVD64_001605"
     CDS             complement(15414..17141)
                     /locus_tag="IVD64_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148860.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IVD64_001606"
     CDS             complement(17157..17807)
                     /locus_tag="IVD64_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6148861.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IVD64_001607"
     CDS             18011..18307
                     /locus_tag="IVD64_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6148862.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18499..19119
                     /locus_tag="IVD64_001608"
     CDS             18499..19119
                     /locus_tag="IVD64_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP6148863.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
BASE COUNT         5456 a         3954 c         3381 g         6395 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aaagaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaat aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gattctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctatgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttcgagtaa actgattttg ttagtcatag ttataacaat catagcaata
    18481 aaaggagaag gtaagcatat ggcatctgtt ttagtagtca aaggtcatcc cctaactgca
    18541 gaagaatcgc gcacagttaa ggcattgacg tcttttttaa caagttataa agaaaatcat
    18601 ccagatgatg aagtgactgt tttggaactt tatcgcgatg atattccgga aatcgatgaa
    18661 gaattattat caggttggga agcattacgt gctggtgcag aattcacttc cttatcagaa
    18721 agtcaacaac aaaaaattgc tcgtttcaat gaattgactg atcagttcct tgctgctgat
    18781 aaaatcgtta tcgcaaacgc cttatggaat ctaaatatcc caacaaaatt aaaagcttgg
    18841 ttcgataccg tcaatgtagc tggaaaaacg ttccgctata cagaaaacgg gccagaagga
    18901 ttagtgacag gtaaaaaagc actgcacatc caatctaatg gcggtgtata caacggacaa
    18961 gactttgcct ctcaatatgt aaaaggtatt ttaaactttg taggcatcga tcaagtagat
    19021 cagctattca tcgaaggaat cgattatgac cctgaccgtg cagatgagtt gatgcaaaat
    19081 gcattagata aagctgctgc tttaggtaaa tcattttaaa aattgagata aatgcataca
    19141 aaagagacag aggaaaaaat cttcaaagca gcatttgtgc cttgga
//
LOCUS       DACBZN010000048        18979 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.048, whole genome shotgun sequence.
ACCESSION   DACBZN010000048 DACBZN010000000
VERSION     DACBZN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.048"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(94..969)
                     /locus_tag="IVD64_001609"
     CDS             complement(94..969)
                     /locus_tag="IVD64_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6148864.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1051..1842)
                     /gene="codY"
                     /locus_tag="IVD64_001610"
     CDS             complement(1051..1842)
                     /gene="codY"
                     /locus_tag="IVD64_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6148865.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="IVD64_001611"
     CDS             complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="IVD64_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6148866.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="IVD64_001612"
     CDS             complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="IVD64_001612"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6148867.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="IVD64_001613"
     CDS             complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="IVD64_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6148868.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="IVD64_001614"
     CDS             complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="IVD64_001614"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6148869.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6307..8385)
                     /gene="topA"
                     /locus_tag="IVD64_001615"
     CDS             complement(6307..8385)
                     /gene="topA"
                     /locus_tag="IVD64_001615"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6148870.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="IVD64_001616"
     CDS             complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="IVD64_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6148871.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9420..10187)
                     /locus_tag="IVD64_001617"
     CDS             complement(9420..10187)
                     /locus_tag="IVD64_001617"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6148872.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="IVD64_001618"
     CDS             complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="IVD64_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6148873.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11300..11698
                     /locus_tag="IVD64_001619"
     CDS             11300..11698
                     /locus_tag="IVD64_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6148874.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            11935..12477
                     /locus_tag="IVD64_001620"
     CDS             11935..12477
                     /locus_tag="IVD64_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6148875.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12560..13111)
                     /gene="lepB"
                     /locus_tag="IVD64_001621"
     CDS             complement(12560..13111)
                     /gene="lepB"
                     /locus_tag="IVD64_001621"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6148876.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(13263..13490)
                     /locus_tag="IVD64_001622"
     CDS             complement(13263..13490)
                     /locus_tag="IVD64_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6148877.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(13487..14005)
                     /gene="msrA"
                     /locus_tag="IVD64_001623"
     CDS             complement(13487..14005)
                     /gene="msrA"
                     /locus_tag="IVD64_001623"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6148878.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(14025..14738)
                     /locus_tag="IVD64_001624"
     CDS             complement(14025..14738)
                     /locus_tag="IVD64_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6148879.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(14741..15637)
                     /locus_tag="IVD64_001625"
     CDS             complement(14741..15637)
                     /locus_tag="IVD64_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6148880.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(15795..16637)
                     /locus_tag="IVD64_001626"
     CDS             complement(15795..16637)
                     /locus_tag="IVD64_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6148881.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            16769..17422
                     /locus_tag="IVD64_001627"
     CDS             16769..17422
                     /locus_tag="IVD64_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6148882.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(17485..18012)
                     /locus_tag="IVD64_001628"
     CDS             complement(17485..18012)
                     /locus_tag="IVD64_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6148883.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(18032..18979)
                     /locus_tag="IVD64_001629"
     CDS             complement(18032..18979)
                     /locus_tag="IVD64_001629"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6148884.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
BASE COUNT         5044 a         3971 c         3333 g         6631 t
ORIGIN      
        1 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
       61 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
      121 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
      181 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
      241 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
      301 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
      361 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
      421 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
      481 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
      541 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
      601 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
      661 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
      721 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
      781 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
      841 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
      901 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
      961 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
     1021 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
     1081 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
     1141 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
     1201 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
     1261 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
     1321 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
     1381 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
     1441 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
     1501 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
     1561 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
     1621 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
     1681 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
     1741 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
     1801 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
     1861 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
     1921 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
     1981 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
     2041 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
     2101 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
     2161 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
     2221 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
     2281 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
     2341 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
     2401 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
     2461 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
     2521 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
     2581 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
     2641 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
     2701 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
     2761 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
     2821 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
     2881 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
     2941 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
     3001 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
     3061 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
     3121 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
     3181 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
     3241 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
     3301 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
     3361 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
     3421 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
     3481 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
     3541 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
     3601 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
     3661 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
     3721 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
     3781 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
     3841 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
     3901 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
     3961 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
     4021 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
     4081 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
     4141 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
     4201 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
     4261 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
     4321 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
     4381 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
     4441 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
     4501 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
     4561 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
     4621 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
     4681 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
     4741 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
     4801 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     4861 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     4921 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     4981 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     5041 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     5101 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     5161 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     5221 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     5281 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     5341 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     5401 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     5461 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     5521 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     5581 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     5641 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     5701 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     5761 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     5821 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     5881 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     5941 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     6001 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     6061 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     6121 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     6181 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     6241 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     6301 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     6361 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     6421 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     6481 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     6541 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     6601 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     6661 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     6721 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     6781 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     6841 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     6901 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     6961 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     7021 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     7081 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     7141 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     7201 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     7261 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     7321 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     7381 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     7441 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     7501 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     7561 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     7621 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     7681 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     7741 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     7801 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     7861 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     7921 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     7981 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     8041 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     8101 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     8161 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     8221 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     8281 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     8341 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     8401 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     8461 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     8521 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     8581 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     8641 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     8701 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     8761 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     8821 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     8881 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     8941 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     9001 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     9061 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     9121 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     9181 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     9241 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     9301 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     9361 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     9421 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     9481 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     9541 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     9601 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
     9661 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     9721 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     9781 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     9841 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     9901 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     9961 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    10021 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    10081 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    10141 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    10201 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    10261 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    10321 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    10381 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    10441 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    10501 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    10561 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    10621 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
    10681 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
    10741 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
    10801 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
    10861 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
    10921 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
    10981 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
    11041 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
    11101 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    11161 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
    11221 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
    11281 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    11341 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    11401 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
    11461 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    11521 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    11581 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    11641 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    11701 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
    11761 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
    11821 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
    11881 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
    11941 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
    12001 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
    12061 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
    12121 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
    12181 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
    12241 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
    12301 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
    12361 gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca aataatggtt
    12421 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
    12481 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
    12541 ctgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga taatagacaa
    12601 atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga ctatcttttg
    12661 aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg gattgtcccg
    12721 ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct gatttcaggt
    12781 ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga tcattttggt
    12841 agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg ccatctgcta
    12901 attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc acaaccatat
    12961 caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg atgaataaaa
    13021 atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat acaagccaga
    13081 agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct ctttttttca
    13141 ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg aaataaaaat
    13201 aattctttca attaggctat tggacaaaaa acttttttga tcataaagta caaaactatc
    13261 tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa gatatccatg
    13321 gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta gctagagctg
    13381 tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa agcatcatgt
    13441 gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc
    13501 tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat
    13561 gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg gctggttcga
    13621 tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc ttactttttt
    13681 cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc
    13741 gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa agttctacta
    13801 attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct gtatgtccag
    13861 tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca gtatatcctg
    13921 acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc
    13981 ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc atccgatgat
    14041 tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt ggtatttgag
    14101 ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat gtcatcattt
    14161 actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa ttgatccaac
    14221 ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa tttatagttt
    14281 ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat agaaagactt
    14341 ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata taaatagaaa
    14401 tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt ttccaaataa
    14461 aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct gatgatttga
    14521 ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct ttccgtaaga
    14581 gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc taaaaatacg
    14641 gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta atttgttttc
    14701 tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca tcctccttct
    14761 ctaaccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc atcaattgat
    14821 aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt ttgatgccat
    14881 ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca cctacttcct
    14941 gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc ggcatttttt
    15001 ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg tcagtgatct
    15061 caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt gcggttttat
    15121 tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt ttcagtggct
    15181 ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc atcaaatcat
    15241 taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga atttcttctt
    15301 ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca tagttttctg
    15361 tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg ggaacgaatc
    15421 ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata gctatgtagt
    15481 gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc attagtgggc
    15541 cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt aataatggga
    15601 acaaaacgag gatcaaatgt cgtttcgtta atttcatttt ttcacctctt gtattcgatt
    15661 ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat
    15721 tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag gctgagggct
    15781 tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt accagtatgc
    15841 gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa taattcttgc
    15901 aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat accgatttgt
    15961 cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt atcgaacgtt
    16021 ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt catcaccacg
    16081 cgtatgttca aaacactaga cagcaaacca gttgttcggc tgattcttcc accttttacc
    16141 aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac ttttttgact
    16201 tcttcaagga ttttctctac tgttttacct tcttttgcta attcagctgc tcgaattacc
    16261 tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt cgtttttgtt
    16321 agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt catatggatc
    16381 gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagatgaa ttctccaatt
    16441 ggtggttggc ttgttttggg cagatttttt gctttcgcca tcatttccat aaatctttcg
    16501 ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga tagcggcatg
    16561 acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga atctgttacg
    16621 atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc ttattcatgt
    16681 taaactacat gtaagacaat ttctattttt agtataacag acaaataatg atttttcatt
    16741 aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga
    16801 aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat tgagtatcgc
    16861 gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc atattgtctc
    16921 ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt tgtttcattc
    16981 attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt cgatttttct
    17041 tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat ggctaggttg
    17101 gactttattt gtattaatct ggttgtttgc gattcttggc attatatata aagcgttgac
    17161 gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt ttatggggtg
    17221 gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatgactg gtgttatttt
    17281 gatggtagca ggcgggcttt cttacacgat aggcgcattc ttttatagct tgaattctat
    17341 ccgctatatg catgttgttt ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt
    17401 ctctgtttta ttctttactt aataaaaaaa ataaagctat ggatgggaca gctctaaata
    17461 acagctagtc tatccatagc ttttttatat cgtcaattcg cctgtcccgc tttttacagg
    17521 attctcatta cgctgatacg tttcaaattg atgcggataa cgattttttt catccatttc
    17581 tccttgacta gtattgatca gtgaccaatc agaccagtca acaggtggaa aataagcatc
    17641 tccatcgaat acatgatgga tgactgtacg gtataatatc gtacaaaatg gcagaaattc
    17701 ttgataaaca gctgaccctc cagcgatgaa aaagataccg tctgtttctt tttccagagc
    17761 aagcacgtct tccacactat gcatgacatg cacgccttct gctttatagg aagaatctct
    17821 cgtcaaaaca atcgtctgac gattcggcaa cggacggcta cccatccctt cgaatgtttt
    17881 tcgtcccatc actagtgtat ttgcttctgt catttgtttg aagaatttca gatcatttgg
    17941 caaacgccaa ggaagacggt cttcttttcc aatcaatccg ttttcatcct gtgcccaaat
    18001 tgcagctaac attttctcat cctttctgat tctaaacagc gataggagct ttgatcgccg
    18061 gatgtgggtc atatccttct aattggatat cttccatctc aaaatcaaat actgactgct
    18121 tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc tcggctcaat tgttctttca
    18181 tttgttcgat atgattgata taaagatgag catctcctaa tgtgtgaaca aattcgccaa
    18241 ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa cgcataactt gcaatgttga
    18301 acggaacgcc tagaaacaca tccccactgc gttgatacaa ttggcagctt agccgtccct
    18361 cttgaacata aaattgaaac atcgtatgac aaggaggcaa agccatgcta ggaacatctt
    18421 ccggattcca tgcagaaacg atcaatcttc tagaatcagg agtctttttg attgcttcga
    18481 tcacttgttt gatttgatcg ataaagccac cgtcttttgt ttcccagtga cgccattgat
    18541 agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct
    18601 tctcgcaaaa aagcttgctc tgctttttat attcttcatt gaattcagga tctgtcaaac
    18661 atctatggcc aaaatcagtc atatctggtc cattatagtc tggacttttg atgtagcgtt
    18721 caaatgccca ttcatcccaa atatggtttt tattttgtaa tagatagcgg atattcgtat
    18781 ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg
    18841 ttaacaaagg aaaccctttt tgcagatcaa accgcatttg gtatccgaat aagcttaatg
    18901 tgcctgtgcc tgttctgtct tctttacgat gcccttcttc caacaatttt cttcctaagt
    18961 caagatacgc ttgttccat
//
LOCUS       DACBZN010000049        18895 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.049, whole genome shotgun sequence.
ACCESSION   DACBZN010000049 DACBZN010000000
VERSION     DACBZN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.049"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(21..251)
                     /locus_tag="IVD64_001630"
     CDS             complement(21..251)
                     /locus_tag="IVD64_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148885.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IVD64_001631"
     CDS             complement(687..1253)
                     /locus_tag="IVD64_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6148886.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IVD64_001632"
     CDS             complement(1253..2209)
                     /locus_tag="IVD64_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP6148887.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IVD64_001633"
     CDS             complement(2209..2865)
                     /locus_tag="IVD64_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6148888.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IVD64_001634"
     CDS             3235..5253
                     /locus_tag="IVD64_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6148889.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IVD64_001635"
     CDS             5680..8094
                     /locus_tag="IVD64_001635"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP6148890.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IVD64_001636"
     CDS             8442..9995
                     /locus_tag="IVD64_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6148891.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IVD64_001637"
     CDS             complement(10376..12160)
                     /locus_tag="IVD64_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148892.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IVD64_001638"
     CDS             complement(12175..13896)
                     /locus_tag="IVD64_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148893.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IVD64_001639"
     CDS             13996..14757
                     /locus_tag="IVD64_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6148894.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IVD64_001640"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IVD64_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="IVD64_001641"
     CDS             16664..>18895
                     /locus_tag="IVD64_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148895.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DACBZN010000050        18149 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.050, whole genome shotgun sequence.
ACCESSION   DACBZN010000050 DACBZN010000000
VERSION     DACBZN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.050"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(218..1015)
                     /locus_tag="IVD64_001642"
     CDS             complement(218..1015)
                     /locus_tag="IVD64_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP6148896.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IVD64_001643"
     CDS             complement(1012..2196)
                     /locus_tag="IVD64_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6148897.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IVD64_001644"
     CDS             complement(2221..3468)
                     /locus_tag="IVD64_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP6148898.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IVD64_001645"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IVD64_001645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP6148899.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDMIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPVALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IVD64_001646"
     CDS             5448..5762
                     /locus_tag="IVD64_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP6148900.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IVD64_001647"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IVD64_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP6148901.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IVD64_001648"
     CDS             6447..7091
                     /locus_tag="IVD64_001648"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP6148902.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IVD64_001649"
     CDS             7106..7435
                     /locus_tag="IVD64_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148903.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IVD64_001650"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IVD64_001650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP6148904.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IVD64_001651"
     CDS             8423..9247
                     /locus_tag="IVD64_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP6148905.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IVD64_001652"
     CDS             9240..9587
                     /locus_tag="IVD64_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP6148906.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IVD64_001653"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IVD64_001653"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP6148907.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IVD64_001654"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IVD64_001654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP6148908.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVD64_001655"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVD64_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP6148909.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVD64_001656"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVD64_001656"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP6148910.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IVD64_001657"
     CDS             15244..16011
                     /locus_tag="IVD64_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148911.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IVD64_001658"
     CDS             16001..18043
                     /locus_tag="IVD64_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6148912.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         5655 a         3332 c         3772 g         5390 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagata tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagttgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttata
//
LOCUS       DACBZN010000051        17218 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.051, whole genome shotgun sequence.
ACCESSION   DACBZN010000051 DACBZN010000000
VERSION     DACBZN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.051"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(105..1124)
                     /locus_tag="IVD64_001659"
     CDS             complement(105..1124)
                     /locus_tag="IVD64_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6148913.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1146..2153)
                     /locus_tag="IVD64_001660"
     CDS             complement(1146..2153)
                     /locus_tag="IVD64_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6148914.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="IVD64_001661"
     CDS             complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="IVD64_001661"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6148915.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2804..5098)
                     /locus_tag="IVD64_001662"
     CDS             complement(2804..5098)
                     /locus_tag="IVD64_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6148916.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5358..7523
                     /locus_tag="IVD64_001663"
     CDS             5358..7523
                     /locus_tag="IVD64_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6148917.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7827..8660
                     /locus_tag="IVD64_001664"
     CDS             7827..8660
                     /locus_tag="IVD64_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP6148918.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8762..10462)
                     /locus_tag="IVD64_001665"
     CDS             complement(8762..10462)
                     /locus_tag="IVD64_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148919.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10969..12129
                     /locus_tag="IVD64_001666"
     CDS             10969..12129
                     /locus_tag="IVD64_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6148920.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12145..12588
                     /locus_tag="IVD64_001667"
     CDS             12145..12588
                     /locus_tag="IVD64_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6148921.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13071..13601)
                     /locus_tag="IVD64_001668"
     CDS             complement(13071..13601)
                     /locus_tag="IVD64_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP6148922.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13706..14257
                     /locus_tag="IVD64_001669"
     CDS             13706..14257
                     /locus_tag="IVD64_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6148923.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14410..14883)
                     /locus_tag="IVD64_001670"
     CDS             complement(14410..14883)
                     /locus_tag="IVD64_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6148924.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15021..16493
                     /locus_tag="IVD64_001671"
     CDS             15021..16493
                     /locus_tag="IVD64_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6148925.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16490..17200
                     /locus_tag="IVD64_001672"
     CDS             16490..17200
                     /locus_tag="IVD64_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP6148926.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5350 a         3097 c         3536 g         5235 t
ORIGIN      
        1 cacgctcttt tttacagata aacaatgtgt tagaagtagt atcttctttt acataaaaag
       61 ctgaccatag gataaatggt cagcgtattt agtgagtcag ctatttagta tgggttttct
      121 aaaggaacga ctgtctctct tttgatcaag cgatgaggga tgaccaaacg tacgccactg
      181 ctcaattctt catggatcaa ttcgcgcagc ttttgcatag cgacacgtcc taattcggag
      241 atatcgatat cgatacttgt aaggtaaggg tgtgttaatg ttgagaagat cgagttgttg
      301 aagctaatga cggagagtgt ctctggaatg acatagccat acatttgcgc caactgcatc
      361 gtgcgaaggg cgaagatatc gtcaatgacc accaatgctg tggcttcgtt ttctttcaac
      421 gttttctcaa aggacagata atcctccgga tgctctagta ctacactgga taacgcagga
      481 agaccagcca tcatcattgc tttttgataa ccgaaatatc gttcgaaata caaactttca
      541 tgagtcgtgt ttgtcacaaa caggatattc ttatgtccat tctggatcaa aaattctgtt
      601 gcttgtttac ctagaagctg gttatcatta tctacataaa cgatttcgtt ctcatgatga
      661 taaggctgtc cgatcaaagt gaaagggatg tggttttggt ataaataatc aatgacagta
      721 tcttgactat ctgaatacac tagaataaaa ccatctactt gtttttgtaa atgcatacgc
      781 tggacgttct ctaacagcaa ttcaaaattt ttagcagtag cgattgctgt agtcatatca
      841 taattccgcg cttcttcgtt gattgcttcc atgatctcaa gataaaatgg gttaccgatc
      901 cgctcttttg aatcaagagg gggtaagatt acgccgatgg cactggaaat gcgttttcct
      961 agattgcggg cagtcatgtt aggaacataa cctagttcat ccataatttt tcggactttc
     1021 ttctttgttg tctcggaaat actaggatga tcgttgatga cacgagagac ggtcgaggtg
     1081 gcaacaccgg cttttttcgc cacatctttt acggtaatag ccatgataaa aacacctttc
     1141 tcttgttatt gtgcagagaa ccgataaatc gtctgactga tccgttcttg accaggatgg
     1201 aggttgatcg atccaaattt ggggaaatga acgatatcag ggatttcttg cggttcgatt
     1261 gctaatccgt agtttgaatg catctgtttt ccattgacag agaaatcagc ttcgaatcca
     1321 gtcgtagaaa ataataccat acttttatta gaagttgcta tagtcatttg gcgaccagaa
     1381 tgcttctcga ataattgtaa ggaagttttg gaaagcctag gatgatgcaa aagaaataca
     1441 tcatctagtc catctggata acatgcaagg atctcttgca gtgttgggga ttttctaaag
     1501 tcgattggca gtttttcttt ttttgtccaa tttccagtag gcagtttttc ttgatctaat
     1561 tcaagcacac catctaaaaa gaccgttaac tgatgttcat agatgtctct tgttccattt
     1621 cctgaaaggt tgaagtaggc atggttggtc ggattgcaga tggtttcaac aaggctacta
     1681 gcagaggttg tcatctctaa ttcgtttttt gttaaacggt aagtgatcgt agcagtgatt
     1741 ggtcctggat aggtagaaaa ttcatctttt aagtaaaaga ccacacctat tgatgatgga
     1801 gttttgaaaa cttctacttc ccaaaattgg aaagaccagc ctttcgtgcc accgtggata
     1861 tggtgtgatc cgcagttttt ctccaatcta tgctcacccc aagaagcatc ccggatcctt
     1921 ccggctacag gtccgacagt tgctccaaaa aagcttttgt ctgttagaac atcttcgaaa
     1981 cgatcgtaag acaataaaac attttctgat tttccttctt tgtctggtgc gaagatttgg
     2041 tgcaggcggg caccgtaatt gagaagaacg acttctaagt agtcgttctt caatgtgatt
     2101 ttctttattt cagtctctgg aactggtacg acggagatct ctggattatc catggtcatg
     2161 ccacttttct ttcaagtaat cgatcgtaag aaaagtcgta tcagggacaa gcgggatctc
     2221 ctctccaagg tcttcagcac gtcccacgcc aattggttga gcgccacttg agaggatggc
     2281 ttggataccg gcttgcgcgt cttcgatccc gatacattct tctgctttta gtccgacagc
     2341 ttttgctgca aggaggaaga tatctggcgc aggttttccg tttgttgctt ctgccgggtc
     2401 ggcaatagca tcaaagtaag gtgtaagacc cattttttca agtaggaatg gcccgttttt
     2461 acttgcagaa gcaagagcaa tcttgatttt ttcttctttt aaagaatcca gtaaaggcac
     2521 gaccccgggg aagacatctt ttggttcgat cgtctggatc atctctaaat aataatcgtt
     2581 ttttcttttc gctaattctg caaattcttc ctttgtaaaa gagtgttctt ttccgccata
     2641 agctaaaagc aaagagagtg agtcttctcg gctgacgcct tttagctgtt cgttgaatgt
     2701 gtggtcgatc gtaatgccga tttcctgacc aagctttcgc caagcacgat aatggaattc
     2761 agcagtatcg gtaatgacac cgtctaaatc aaacaatacc cctttaaaca tttgcttcct
     2821 cctttcttag cgttatttct agacgatccg ctaaagaata agactgttca tagacgtaaa
     2881 tttctagagg agaaccttct aataacttga aagtaacttg ttcatctgtg actgagatga
     2941 acaataaacg tccgcgataa ttgatatgga aagaataagc tttccaagtt tttggcagaa
     3001 atggtgcgaa tctcaactga tcatgggcag ttttcatttg agcaaatcct tggacaatcg
     3061 acaaccagct accagtcatg gaagtgatgt gcaagccgtc ttctgtgtcg ttattgtaat
     3121 tatccaaatc caagcgagct gttcgctggt acatttctac agctttgctt tccattccta
     3181 attctgctgc taaaatcgaa tggatacttg gcgaaaggga agactcatgg acagtcattg
     3241 gttcatagaa atcaaagttt ttttgctttt cttcttttgt aaaagtatca ttgaagaaat
     3301 aaatcccctg tagtacatct gcctgcttga tgaagcaaga tctaaggatt ttgtcccaag
     3361 accaatgctg attcagagga agatcgcttg tgggaatatc ggatacaggc atgagatctt
     3421 tatctaagaa tgtgtcatgc tggacaaaga ttcctaattc ttcgtcaact gggaagtaca
     3481 tgttctctgc gatatctttc catttggcta gttcttcttc gctgatagtg atagcagctt
     3541 ctttttgata agttaaataa ttttctcgcg tgtaacgcaa gacccaagct gcgatcgtat
     3601 ttgtatacca attgttattg atattgttct catattcgtt tgggccggtc acaccgtgga
     3661 tcatgtattt atttgctcgt tttgaaaaat ggacacggtc tgcccagaag cgtgcgattt
     3721 ctgctaaaac ttccagtcct tcatttttca aatattccgt atcgccggtg taattcgtat
     3781 aattgtagat tgcataggca atcgcgccat ttcggtgaat ctcctcgaat gtgatttccc
     3841 actcgttatg gcattcgaca ccagtgaagg tgaccatagg ataaagcgcg ccttttaatc
     3901 cttgttgctg agcgttgtgg attgcttgcg gcagttgatt atgccgatat ttcagcagat
     3961 tcttagttac ttcaggtttg gctaaagcaa gataaagagg gacagcatac gcttctgtat
     4021 cccagtaagt ggctccgccg tatttttctc ctgtgaatcc tttggggccg atattcaatc
     4081 gttcatcttc gccgtagtaa gtagagaata gttggaacaa attgaatcga ataccttgtt
     4141 gtgcttcttc gtctccttcg atgaccacat cggcaagctt ccatcgttct gcccaagccg
     4201 ctgtttgctc ttcttttgct tggatataat tgtttgtctg gttcgttaat tctgagacag
     4261 ctttttctgc ttgctgcgct tctggaatat ctctactagt aagaacgata atttcttttt
     4321 ccactgacca tgtctcacct tgagataatt ctgcagacaa gcaaccatgt aatgctaaag
     4381 tatcttcgtg atagtcaggg atgactgatt ttccattaac gaaatggcgc attgctgcag
     4441 taacagtaaa ttgttcaatc ccaaagttgt ttggaatagt tttagtcgtc aaatagccga
     4501 tttcttctat agttccacga tcaatctcat tccaaaaatg ttcatcatag ttgctgtctt
     4561 cgttttgaac atttccatcc aattttgata cgagtttgac tgttccgcta ccttcaagca
     4621 tttctatagt taaatagata taagctgatt cttttttgac aatgctcaga aaacgttcga
     4681 aggaaatgcg tactttatta gtagaagtag tgattgtgaa tgtacgagat aatacaccat
     4741 ttttcatatc taattcccag taaaagtctt ctggttccaa agaagccaag tcgatcagct
     4801 gatcgttgat cgaaatatct atcgcgataa agttcaaggc gttgatcact tttccaaagt
     4861 attctggata accatttttc caccagccga cacgtgtctt atcgggatac cagacacccg
     4921 ccaaataagt accttgatgg tggtcccctg agtaagtttc ttcaaagttc ccgcgcatcc
     4981 ccatataacc gtttccaata cttgttaaag attcttgcag tcgaagattt tttttatcta
     5041 attgtgtcgt gctgattttc catggatcaa tagcaaatag tcgtttgatc tgtttcattt
     5101 ttctcctcct aatgagctgt tttatacgtt cataataaac gcaaccgatt gcacaagtca
     5161 atagacaaaa gaaaatattt catagaaaaa agcttaaaaa aataaatttt taggagaaaa
     5221 ttatagaagg gaacacacgt attataggtt ttatagaatg gtatagacag aaaaataaaa
     5281 aataaagcat ttacaaaaaa gaaaacgcac gctataatac gtttaacgaa accgattgcg
     5341 taaaggagct aaacggaatg aaaaagatat ttagttttga gttttggcaa aaatttggaa
     5401 aggctttaat ggttgtggta gcagttatgc cggcagctgg gttgatgatc agtataggga
     5461 aatcaattcc cttgatcaat cctgattgga cgccgcttgt cacaacgggc ggagtaattg
     5521 aaaatattgg ttgggcaatt attggtaatc ttcatatttt atttgcttta gcaataggag
     5581 gaagttgggc aaaagaaaga gcaggcggag catttgcagc tgggatctca tttattttaa
     5641 tcaatcggat cactggggct atctttggtg tcacaagtga catgcttgcg aatgaagatg
     5701 catttaccca cactctgttc ggtacgaaga tcatggttaa aggatttttt acaagtgtat
     5761 tggaagcacc agctttgaat atgggagtat tcgtagggat catcgctgga tttgtcgggg
     5821 caatggctta taataagtac tataattata gaaaattgcc ggatgctttg tcatttttca
     5881 atggaaagcg ctttgttccg tttgttgtta tcttatggtc aacaatcgtg gcattgattc
     5941 ttgctgtggt gtggccaaac gtacaggctg gaatcaataa ttttggttta tggattgcag
     6001 aatcacaaga aagcgcaccg attctagcac cgtttttatt tggaacatta gaacgtttac
     6061 tattgccatt tggattgcat catatgctga cgattcctat caactataca cagcttggcg
     6121 gatcgtatga gattttatca ggcgcacagg cggggacaca agtatttggg caagatccgt
     6181 tatggttggc ttgggccact gatttagtga atcttaaagg tgcaggggat atgtcgcaat
     6241 atgagtttgt gttgcataac tggacgccgg ctcgtttcaa agtaggacaa atgattggtt
     6301 cttcaggtat cttgatggga atggcttttg cgatgtatcg taatgtcgat cctgataaga
     6361 aagcaagata taaatcaatg tatttttcag cagcgttggc tgtctttcta acaggtgtaa
     6421 cagaaccatt ggagttcatg tttatgtttg ctgctgtgcc attatatgtc atttattcag
     6481 tgatccaagg ggctgcattt gcgatggcag atatcttgcc attgcgggtc cattcatttg
     6541 ggaatatcga attgctgact cggacaccgt tagctatcaa agcgggtcta ggtggagatc
     6601 ttatcaactt cgtgttgtgt gtgatcgctt ttggtgttgt tacgtatttc ttagcaaatt
     6661 tcttgatcaa aaaattcaac tttgctacac cgggacgtaa cggaaattac gacaatgaca
     6721 gtgaagaaac cgttgtatct aacagcggaa caggaacagc agatcagcaa gtggtccaga
     6781 tcattcattt gctgggagga aaagaaaata tcaaagatgt cgatgcttgt atgactcggt
     6841 tacgcgtaag tgtaaatgat cgagaaaaag tagggactga agaagcctgg aaacgagcag
     6901 gggcgatggg attgatcgtc aaagacaatg gagtccaagc agtttatgga ccaaaagcag
     6961 atgtcttgaa atcagatatc gaagatctgc tgcaatcagg agcagagatt ccagagccag
     7021 aaatggtttc tacaggaaat ggacaagcag gtagtaagca atacttaggt atagaacaag
     7081 aactagtttc cgcagcttct ggagaagtga ttcctttatc tgaagtaaaa gatccggtat
     7141 tctcacaaaa aatgatgggt gacggctttg ctgttattcc aacatcaaga gaagttgttg
     7201 cgccgatcgc tggaaaagtg acaagtattt tccctagcaa acatgcaatt gggctggaaa
     7261 cgaaagatgg tatcgaagtt ttgatccata tgggaatcga tacagtccaa atgaatcagc
     7321 cagcttttga gattgctgtc aaagaaggcc aagaagtagg aacaggaacg aaattagctg
     7381 agatggatct tgatgtgatc caaaacgaag aaaaagatcc aacgatcatg atcgtattta
     7441 caaacgataa agtagaagaa gtagtgatca agcaattagg tacagtgaca gctggtaaag
     7501 tgatcggctc cattaaaatg taaggagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     7561 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgag
     7621 aagcagcagt ttgaatactg tttagtggag aggtcgtgaa aaagctgccc tgcaccaagt
     7681 gataaaaaca gccaaacaaa aaatagctcc tggtccattg agcttattac tgaggagtta
     7741 gcaggagaac actatttgct ggagaggttg tgacaaaaat tttgtcacaa cccttttgtt
     7801 tcgaaatgaa gacaaggaga aaaccaatga aattcatgac actcaacacg catagctggc
     7861 ttgagccaga accagagaaa aaattgcaag aattagcaga taagatcctg ttagaagatt
     7921 atgaagtgat tgctttacaa gaaatcaacc aattactgga atctgaagaa gtagaacctg
     7981 cgaagctaat gaagttctgt ccggtaaaga atcaagtacc tatccgtaaa gataattttg
     8041 cctaccgttt agttcagctt ttaaaggaac acggaaaaga gtatttttgg agttgggaaa
     8101 tgagccacat cggttatgat aaatatgaag aaggaaatgc actgcttacg aaaaaaacat
     8161 tggaaagtca ggtccttacc gtctcccaat ttcaaaagaa agaagattat cagacacgca
     8221 agatcttgat tgggaaaaca aagatagatg atcaggacat ttttgtctcg agctgccact
     8281 tttcttggtg gacagataaa aaaagtggct tttattttga gtggaaaaac ttggaaaact
     8341 actttttgga aactcgcgta ccactttttt tcctaggtga tttcaataat ccagtagact
     8401 ctcaaggtta ttatactgta agagaaagtt gccttcttct tcaagattcg tatgttgtcg
     8461 cgaatgaaaa aggaaaagca gctactgtgg aaaagaaaat agatggttgg gaacaaaata
     8521 cagaaaaatt gcgtatagat tttattttcg tccctgaagg aatgcaggtt aaaaaatatc
     8581 aacggatttt tgatgggatc gatagtccaa tcatcagtga tcactacggg gtggaaatcg
     8641 aattagatgt gaacgaataa aaaatataac atataacata aaaaaactga ttaaaataaa
     8701 actctccaaa cgattatacc gtttggagag ttttttgatc ggatgtattt atcccttttg
     8761 cctaagcacg taaaagcttg tatctatgat aaataaataa acatagcaag gtaaaccagc
     8821 caatcaaagt aagagaaaga atgaaataga aatagattgg cgtttgataa tgaagcgtga
     8881 gctcgttgat tcctttttgc tgcttcacga ttggggtccc gatatctgtt acttcataat
     8941 cagtcaatgt ttttccattt tgctgtagca cagtccgatc gtagacgata acagggatat
     9001 gaacagtttt gtccccagtg tttttccact tgattgtcaa aagaccgcct ggatgtgttt
     9061 ttataaaagt tgtttcgttt tctagtattt cttcgttata ccggtcgtat ttattgtctt
     9121 ttgtctcctt atagattggc agatagtcag gtgtggtttt ttggaaaacg aacaagctgg
     9181 tagacatatc tttgctgaag aatgttttcc ttgcttcttc cggcgtatcg aatagataag
     9241 tatgtcgact gacaaatgtt tcattttccc acttttccag atgctggtat aagttctgga
     9301 tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa aagacggaac cacttttgtt
     9361 cagaccactg gttcagtgaa agggcaaggt aaagtagaag caatagcgta aatggcacga
     9421 aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac atttttttcg atcaatgtcg
     9481 tccatggaat caagctagta gaaagaaaaa agaaaacaag acttgtgtac aacacgattt
     9541 tttggaataa ggtgttcttt tgttgaaaga aaataaaaag caaaaaacct gataatatca
     9601 atataggaaa ggccttaggg taatggagcc aataactgcc atccagatct atcgcctttt
     9661 gactcatttc tctgttaaca aagggaggaa gaatcttatt cccactgtaa acaatagcta
     9721 atcctgccca aatatttgct gtcagaacaa aataaagcaa gaccgactta atcagttgaa
     9781 gcagtgcttt tcctttttca gtctggttaa aaaagtaata aagataaaaa gggatataca
     9841 tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt cataagtccc actgagacag
     9901 cgactttgat aacagggaac tttttatgaa ggacgaaatc agcaataggg atcatacata
     9961 aaggataaaa acaagtcccc cagctagtga atccttggtt gatagaccag tattgaatag
    10021 aatacgtact catgaataaa acggctactg gtacacttac ccaatttcga acattgacat
    10081 atctcagtaa aatcagcata gaaaatcctg aaataagata aagaatgaaa tttgaaagaa
    10141 tctgataatg aaaccagcta cctgcaagca acacgatcaa tccttgtaaa taagcgaaaa
    10201 atgggccgta aacggcattc acgatcctgc cactttgctg gaatccataa agggaaggaa
    10261 aataatggaa atccccatat ttaatttgtt gcgcggcatc atagaaccga ttgtaatgga
    10321 atggggcatc agcacccaaa attagtttat gtgtgaataa atgaggcatg agaaatgcca
    10381 aagcaatgaa aaaaattgct aaaaatgggc catatttttt caattgtcga actcctttgc
    10441 aaactttaga taaattcatc atatatccta tgcgagaggt aggcaagaaa ttagaaaatt
    10501 catgaaaata tttatgaatt ttacaagaaa aacataagtg aaagttctaa ggagagagga
    10561 tgcgacataa gttttgttgc atctccttgt tttgaaacag aatttttctt aatactcgaa
    10621 acaaagtagc tttttcatga cctctcggta aacggtgttc aatcagctgc ttctcggtaa
    10681 taagctcact agcctagaaa atatgagaag ctattttctg actagcgctc ttattactca
    10741 aagcataacg cagttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    10801 gtaataagcc caacccaaca aaaaatatga gtagctattt ttttgggctg tacttattac
    10861 ttgatgcagg acagcttttt cacaacctca tcggctttca ttttctattt ttttcctgaa
    10921 cccatataat aaaagaaaga acaaaaaaac ttgcaaggga ggacacatgt gaagcagagt
    10981 aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt tattaatatg tatcgtcgta
    11041 gggttactga tcgctttata tccattttat gtggattctt taaatagttt tattgatcaa
    11101 aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa ttgaagtgca gcgaaagaaa
    11161 atggaggaga aaaaccaacg attaactgac caaggattca atcctggtac tgatcctttc
    11221 aatgaaaaga acagaaatga aagtacaaca tccagccaat tagaggaatg gctgatcgga
    11281 agtatcaata tacctaagat ccaattaaat atttcacttt atgatcggct caatggtagg
    11341 atccttgaaa atggagcagg cgtcttgcaa ggaacctctt tcccgcttgg tggaaattcg
    11401 acgcattcag tgatctctgc tcacagcggt ttacctaatc gacggttatt taccgaatta
    11461 gatcgtttag aaaacggaga cacgtttatt ttgactgttt tgggggagaa attggcttat
    11521 caagttaaaa atatacaagt ggtactgcca gatgacacgt ctgttctaac gatttcagaa
    11581 ggagaagact tagttacctt gcttacatgt acgccgtata tgatcaacac gcatcgtttg
    11641 ctagtgacag gacatcggat tccttataac gaatcggtaa aaaaagaaga aggaaaagga
    11701 aatcaggaaa gaaccatgcg gcaatggttt attcttgcag gaacgataat agctgtagtc
    11761 atactgttag tgtttatcgg acgactaatc tatcagtata gattaagtaa aaaagtgctt
    11821 gatttttcat ttattattag tgattcgtca gggaaaccgg taaatggtag ttcattcatt
    11881 ttgaaacata agaaaaaaac acttactaga aatggtgtgc cgttttcagt acaaagcgac
    11941 catcatggga aagtcaagct ggatcaattg cctggtggaa cttatcgaat cgtttcagct
    12001 gatgatccta aagttgctgt ttcttttggt attagaaaag taaaacaaga gaagatatac
    12061 ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa atggttttta ttttaaaatc
    12121 aatgaataag taaggagaga aaaaatgatc cgcacaacaa caaaattgtc tgccaaagaa
    12181 ctggaatctg ttctatcgat ttggctgcat gctaacttgg attctcatga attcattcca
    12241 tcaacttatt gggagaatca agtagaatct gttaggaaag ctttaccaga tgcagtgatt
    12301 tacttagcag aaatagatca agaaattatt ggttttgctg gggtgattga tcagtttatc
    12361 gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa tcggtacgca actaatgaaa
    12421 aaaataaaag aaaccaataa aacactgact ttgacagtct atcaaaaaaa tcaatcagct
    12481 attcatttct atctttcaca aggttttaca attcaaacac agcaaaaaga tgaagcagta
    12541 aatgaggagg aacttgtgat gaaatggaat caaaacgctg atttttgatg aacagaataa
    12601 cgaaataatg ctcccagcct aattttttgc tgggagcatt atttcatttt gaagtcacta
    12661 atttaactgt gtgaatatgc agttgttaca atctttgata aacggtgttc aatcagctga
    12721 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttgtttctc
    12781 taatactcag gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt caaacggctg
    12841 cttctcggta ataagcccac tagcctagaa aatatgaaaa gctattttct aactagcgtt
    12901 cttattactc aaagcataac gctgatttca caacctcttg ataaacggtg ttcaatcagc
    12961 tgaccctcgg tattagctca aaaaacggca aaacatgagg aactattttt ccgtttcttt
    13021 ctctaatact caggtcagaa cgctgatttc acaacctctc tttcacaacc ttattttact
    13081 cgtttataat tttcatcgaa gtattttccg ctacggatat catctggcat tccatcgtat
    13141 ggttcttcgc taataacatc ttcattgtct atacctgcat cacccatata agtgatcgta
    13201 gcaaattcag gaacaagtaa ttttggataa gtttcatatt tttcacgagc tgcttccatt
    13261 tctgcgatat gttcgttttg gaaagtaaca gtgatttctg gatgttcttc aacccattta
    13321 gggaaaaaga tcattccgtg aagtttcttt tcatttggca taaagtccaa tgccacgtct
    13381 gtccctgttg gctctgtcca agtgattttg aatacgccgt ccgtcaattt aacgatatca
    13441 gcttcttggt cttttaccca acggcctttg accattccgc catggatacg gtaatcaaca
    13501 gtatggtcat ttttggcata ccattcatat tcccaaccat tgtcatacgt atagataaag
    13561 tgagttccaa taaaatcttc caattcttta aattctttca tgtttaagca tctccttata
    13621 aattaaaaca tgtaataaac tacatgtttt cgattactca tttatgatag aatggattta
    13681 aagatttgtc aaggagggaa aactgatggc aagaaaaaat acacttcgat atattttgtt
    13741 agggttatta agtaagaaga agatgacagg atatgaattg aatcaatcgt tcaaaaatga
    13801 aataggagaa ttttggcaag caaaacatag tcagatttat cctgaacttg caaaaatgga
    13861 agagcaagga atcattcagc atcaagtaga gatcacaggt gaaaaattgg aaaagaaagt
    13921 ttatgaatta acagaagaag ggaaggaaca attggcagag tggattcata cacccaccaa
    13981 tgaactaccg gttaatcgag acgaatttgt tctgaaattg tatttcgtca aagacgtgaa
    14041 tgatccggca ttagtagaaa ttattcagca gcagcgtgac cttcatgaag aaaaacgact
    14101 tcatctatta gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac atgaaaatta
    14161 cggacactac ctgattttag cgcatgcgat caatcgggaa acagaatatg caaattggct
    14221 aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa cagtgatgga
    14281 gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg gatattctta tgttcaaaca
    14341 ggcctttttt caagtattta tgtagcatct acttattaaa aagcttggta cttaggagat
    14401 tagtggcgtt tagagttctt ttaacaactg ttcgtagtca gctttttcat cgacaggaag
    14461 gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct tagcattctc
    14521 ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt cgatatcttt
    14581 gattgctttt gaaaaaactt tacggacatg gtgcattttc ttttggcgga tttctgcctt
    14641 ttctgcagct tttgacaaag taaatccgtc atctaaatat tttttgatca gttctacttt
    14701 tatgactgta ggtaaacgat attttctagg acaattcgca tcagcttcga tcgaacgaat
    14761 gaatcccttt tgttcccaat accttaattg acgtggagac acacctacga tatcacttaa
    14821 ttcactgatg ccaacaacca attgatcatt ttccaacaaa cgtttaaggg aagccccaac
    14881 catttttgtc actccttttg tcctcttgct tcccttaatt atatccgtat taacttatgt
    14941 gtcaagtagg ttgacaaata gcgaatttag gactaaagta ttctagtagt ttcagaaaaa
    15001 attcagaaag aaggagaaat atgagtaaca accaacccgt agatatttac ggaaaaccat
    15061 ataatcggac acttttagtt gttgtcttat taattggtac attttgtacc gttttgaatc
    15121 agaccttatt gacgacagct tttcctacat tgatgaaagc tttcgatatt tcagcgtctg
    15181 atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg gatcatgatc ccaataacag
    15241 catggctgat caataaattc agttcaagaa atttatatct ttctgccatg acgatctttt
    15301 tgatcggaac gattacttgc tttacagcac ctaattttag tacattacta atcggtcgtt
    15361 tgatccaagc ttgcggtgtg ggagtatcga tgccattatt gcaaaatatc atgctatcta
    15421 ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt attggacttg
    15481 ctcctgcttt aggccctaca ttatctggtt atatcattga taactaccat tggcgtgact
    15541 tgtttggtat ggtactgcca atcgttgttc ttgtcttggt tttagcattc ttcctaatga
    15601 aaagtgttat tccattgtca aatcctagca ttgatatttt atccgctatt ttatcaacga
    15661 ttggattcgg tagtttactt tacggatttt ctagtgtagg agatgacggc tggggaagct
    15721 tgaaagtaat tggtttctta gttgttggtg tgattttcat cgccttgttc tcatggcgtc
    15781 aattacattt ggaacatcca ttcttggaac ttcgtgtatt caaatcatct acattcacga
    15841 ttgcagctgt tttagctggt gtaacaaata tggcgatggt aggggcagaa atggtcgtac
    15901 cattgtatat tcaaaatatt cgtggagaat cagcattcca ttctggcttg atgttgttgc
    15961 cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg tgcgattttc gataaatacg
    16021 gcgcgaaacg tttagccatc acagggatgt tcattttgac agcggcaact gcaccatttg
    16081 ctttcttgac aaaaacgact ccagttttat atatcgtgat cctttatgcg atccgtatgt
    16141 ttgggatctc catggtcatg atgccggtaa cgacttctgg tatgaatgca ttgccaaata
    16201 acttgatcag tcatgggaca gcggtaaata atacattccg tcaaatcgca agctcgattg
    16261 gtacagctat tttgatcagt gtgttgacga atgtgacaaa agcaaatatc ccaagcaaat
    16321 ctgtattgga taatacgcca ttagcttata aagatcaggc aatcaatgcc acactgtcag
    16381 gttaccatgc cgcgtttatc gtagcgatca tctttgggat tcttggcttt attattgcct
    16441 ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca tgattattgg
    16501 agttttgatt gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta aaaaaacatg
    16561 gcaaaccttt ttatctttga ttttcggact aatcttcgtc gcttcactaa gtttgatcgt
    16621 cgctaatttg tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa cagaaaaaat
    16681 tgtttccagt gctgattccc aaggagcaga tatgcttttg tataaagctt tagggaatgg
    16741 aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa aagcaactgg
    16801 tacggataac gaaacaaata aagtagaaaa gacagacggc gatgctgaaa aagtgacgaa
    16861 aactacttat tgggaataca aaaacgacat gtataaattc tggttcaata ttgctgataa
    16921 caatcacgaa tatgacagcc gagtgaatac attcaaaatc ccagagacat gggtagaatt
    16981 aagtacggac caagcagcca agttagctga attagtgaag aaacagcaaa gtacgatgga
    17041 aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg aaaatccaaa
    17101 aatgagcaaa gcagagcaag aacaacgtac gaaagcctta gctgctgaat tccaacaaca
    17161 agcattcgca aaattagtga aagaagcaaa aggcgaataa tattttgaac accatgta
//
LOCUS       DACBZN010000052        17107 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.052, whole genome shotgun sequence.
ACCESSION   DACBZN010000052 DACBZN010000000
VERSION     DACBZN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.052"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            7..1398
                     /locus_tag="IVD64_001673"
     CDS             7..1398
                     /locus_tag="IVD64_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6148927.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IVD64_001674"
     CDS             1414..2454
                     /locus_tag="IVD64_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6148928.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="IVD64_001675"
     CDS             2473..3558
                     /locus_tag="IVD64_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP6148929.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="IVD64_001676"
     CDS             3591..4553
                     /locus_tag="IVD64_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6148930.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="IVD64_001677"
     CDS             4546..5979
                     /locus_tag="IVD64_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP6148931.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="IVD64_001678"
     CDS             5976..7046
                     /locus_tag="IVD64_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148932.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="IVD64_001679"
     CDS             7033..8454
                     /locus_tag="IVD64_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6148933.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="IVD64_001680"
     CDS             8467..9474
                     /locus_tag="IVD64_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6148934.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="IVD64_001681"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="IVD64_001681"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP6148935.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="IVD64_001682"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="IVD64_001682"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP6148936.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="IVD64_001683"
     CDS             11608..12285
                     /locus_tag="IVD64_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6148937.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="IVD64_001684"
     CDS             12275..13417
                     /locus_tag="IVD64_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP6148938.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="IVD64_001685"
     CDS             13430..14407
                     /locus_tag="IVD64_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6148939.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="IVD64_001686"
     CDS             14407..15315
                     /locus_tag="IVD64_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6148940.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="IVD64_001687"
     CDS             15316..16068
                     /locus_tag="IVD64_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP6148941.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="IVD64_001688"
     CDS             16073..17020
                     /locus_tag="IVD64_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6148942.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6164 a         2190 c         3090 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaa
//
LOCUS       DACBZN010000053        17005 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.053, whole genome shotgun sequence.
ACCESSION   DACBZN010000053 DACBZN010000000
VERSION     DACBZN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.053"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..125)
                     /locus_tag="IVD64_001689"
     CDS             complement(<1..125)
                     /locus_tag="IVD64_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP6148943.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="IVD64_001690"
     CDS             complement(229..540)
                     /locus_tag="IVD64_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6148944.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="IVD64_001691"
     CDS             complement(542..739)
                     /locus_tag="IVD64_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP6148945.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="IVD64_001692"
     CDS             complement(1133..2860)
                     /locus_tag="IVD64_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6148946.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="IVD64_001693"
     CDS             complement(2853..4046)
                     /locus_tag="IVD64_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6148947.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="IVD64_001694"
     CDS             complement(4233..4877)
                     /locus_tag="IVD64_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6148948.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="IVD64_001695"
     CDS             complement(4971..5105)
                     /locus_tag="IVD64_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP6148949.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IVD64_001696"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IVD64_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP6148950.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="IVD64_001697"
     CDS             complement(7236..7709)
                     /locus_tag="IVD64_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP6148951.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="IVD64_001698"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="IVD64_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP6148952.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="IVD64_001699"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="IVD64_001699"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP6148953.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="IVD64_001700"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="IVD64_001700"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP6148954.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="IVD64_001701"
     CDS             complement(11756..12895)
                     /locus_tag="IVD64_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6148955.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="IVD64_001702"
     CDS             complement(13204..13917)
                     /locus_tag="IVD64_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP6148956.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IVD64_001703"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IVD64_001703"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP6148957.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="IVD64_001704"
     CDS             15105..16637
                     /locus_tag="IVD64_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6148958.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="IVD64_001705"
     CDS             complement(16828..>17005)
                     /locus_tag="IVD64_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6148959.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3494 c         2910 g         5322 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga aaaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DACBZN010000054        16903 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.054, whole genome shotgun sequence.
ACCESSION   DACBZN010000054 DACBZN010000000
VERSION     DACBZN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.054"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..189)
                     /locus_tag="IVD64_001706"
     CDS             complement(<1..189)
                     /locus_tag="IVD64_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148960.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(199..1014)
                     /locus_tag="IVD64_001707"
     CDS             complement(199..1014)
                     /locus_tag="IVD64_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6148961.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1276..1719)
                     /locus_tag="IVD64_001708"
     CDS             complement(1276..1719)
                     /locus_tag="IVD64_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP6148962.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1853..2191)
                     /locus_tag="IVD64_001709"
     CDS             complement(1853..2191)
                     /locus_tag="IVD64_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6148963.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2179..2556)
                     /locus_tag="IVD64_001710"
     CDS             complement(2179..2556)
                     /locus_tag="IVD64_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6148964.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2780..3973
                     /locus_tag="IVD64_001711"
     CDS             2780..3973
                     /locus_tag="IVD64_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6148965.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGPSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4139..4561)
                     /locus_tag="IVD64_001712"
     CDS             complement(4139..4561)
                     /locus_tag="IVD64_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6148966.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4956..5150
                     /locus_tag="IVD64_001713"
     CDS             4956..5150
                     /locus_tag="IVD64_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148967.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5147..5641
                     /locus_tag="IVD64_001714"
     CDS             5147..5641
                     /locus_tag="IVD64_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148968.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(5803..6975)
                     /locus_tag="IVD64_001715"
     CDS             complement(5803..6975)
                     /locus_tag="IVD64_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6148969.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7181..7996)
                     /locus_tag="IVD64_001716"
     CDS             complement(7181..7996)
                     /locus_tag="IVD64_001716"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6148970.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNMI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8146..8592)
                     /locus_tag="IVD64_001717"
     CDS             complement(8146..8592)
                     /locus_tag="IVD64_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6148971.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8589..9593)
                     /locus_tag="IVD64_001718"
     CDS             complement(8589..9593)
                     /locus_tag="IVD64_001718"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6148972.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9633..9962)
                     /locus_tag="IVD64_001719"
     CDS             complement(9633..9962)
                     /locus_tag="IVD64_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6148973.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10088..12415)
                     /locus_tag="IVD64_001720"
     CDS             complement(10088..12415)
                     /locus_tag="IVD64_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6148974.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(13258..13551)
                     /locus_tag="IVD64_001721"
     CDS             complement(13258..13551)
                     /locus_tag="IVD64_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP6148975.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(13954..15132)
                     /locus_tag="IVD64_001722"
     CDS             complement(13954..15132)
                     /locus_tag="IVD64_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6148976.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(15236..15550)
                     /locus_tag="IVD64_001723"
     CDS             complement(15236..15550)
                     /locus_tag="IVD64_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6148977.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            15657..15872
                     /locus_tag="IVD64_001724"
     CDS             15657..15872
                     /locus_tag="IVD64_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148978.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(16172..16414)
                     /locus_tag="IVD64_001725"
     CDS             complement(16172..16414)
                     /locus_tag="IVD64_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6148979.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(16401..16829)
                     /locus_tag="IVD64_001726"
     CDS             complement(16401..16829)
                     /locus_tag="IVD64_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6148980.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFLI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         4927 a         3272 c         2936 g         5768 t
ORIGIN      
        1 gcaacctgga ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc ttccatctat
       61 cgacaaaagg aacgcagctg ctatatacaa gatattgaca caccaaaaac aaaggctact
      121 tgccaatgat tgatacaacc aataaaagaa ttccatataa acatttttgt caaaacgctg
      181 ctctttcata atcacctcct attccttcat tcctgatgaa gcgatccctt ctacaaaata
      241 tttttgcatc gctaaaaata taaaagcaac aggaagaaca gaaacaactg aggcagccat
      301 gattaatgcg tactctgcat caaatagccc aataaacatt ttcaacccta actggattgt
      361 tttattttcc gtagaagaca aataaatgaa tggccccata taatcgttcc atgtattaac
      421 gaatgtgatg atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg
      481 atagattccc cactctgata agccatcgat ccgcgcactc tcacataaag aatctgggat
      541 actactgtag tattgcttca acaagaacac accaaaagca ttgaacgctt gtaatagcac
      601 caacgaccac aacgtatatg ttaaacccac ttttctcatc ataatgaact gcgggatcat
      661 ataagactgc caagggacag caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc
      721 tttaaaattt agtttagaaa acccgtaagc agcaaaacta gaagtaaaca actgaatcaa
      781 cgtgatcaca acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag gaatacgaga
      841 ccaaataacc agataattct ctaaatgcca agttttaggg atccattgaa tcggaatcgt
      901 gaacacttca ttatttgctt tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt
      961 caataaagct aatacaatca gcagaatcat acccaaccat tgaataaagg gcatgttttt
     1021 tgaacgaatt tttgcttttt ttctttcatt tcgaacacct ccaaagttat aatcttcatt
     1081 tctttgtaat gattacaatc cagaacaacg gttattatag ctgattcatt tccgttcttc
     1141 ttatgtttgt aagcgcttat ttaacaagtt atgtaaatct ctttattttt tttgatttaa
     1201 aaatgaaaaa atacaaaagc aagactgtca tacgtctcgc ttttgtattg aaatttcttc
     1261 gattagagat acttattact gttcaaaagc ttctttgaaa cggtgaatca attcattgac
     1321 tacaggtgat aaaatccgat tctttttcca gactagcatg cagttcgttt caatcattgg
     1381 gtcaagcgga acgaatgtca ctcgctttga aagatgactt acagcacctt ctatgactaa
     1441 agcagcacct acctgattct caattagcgc aaatacattg aaaatcaaat tgtacgttcc
     1501 tgtgatcagc aactgctcct tttttatgcc taaccagtca gcaaacattc gctgtatttc
     1561 tggacgcttt gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt
     1621 ttttgctaaa acagaatttt ctaaaatgag caatccccat ttttcctttc ttggtaacaa
     1681 aatcgtttcg tatttctctg tatctactgg atctagcaaa atcgctaaat ctaacaggct
     1741 tttttctagt cactccgtga tatcatcact tgttccgctg tggatatgaa attgaacgct
     1801 gggataatca cggatcaact tttctagtaa tagtgccaat gtattcgaat tatcagcttc
     1861 cacacagccg atcgtaaatc ggccgctgaa taattgtttt tttctgttct cgaattcttg
     1921 agtggtttga ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga aaataacctg
     1981 cttctgtcaa gatcattttg cggttttcgc gtacaaacag ttctgttcct agttcctctt
     2041 ctaactcctt cagttgtcgg cttaaggttg gttgtgtaag atgtagaact tctgcggctt
     2101 gagagattgt tccttcttct gcaattgtcc aaaaatattt tagtaagcgt aactccatag
     2161 cgatgctcct tgattttttt acttgataca ttccatttta tttctagata acacacgatc
     2221 atatttcttg atttttcctt gtaaacgttc caaacactct tgcatttcag caatttcttt
     2281 cgtcaaaagt tcactttctt caatcaaaag cttacgccgc tcaaatacag ttgcttctcc
     2341 ttcgctagat aggcgaatat attcaatcaa agactcgatc gataatccgg catttcgcat
     2401 acagatcgta aagtcgaccc attttaggtc ttcttctgta taatcacgga ttccgccttt
     2461 tgtccgatta actggtggaa tcaacccgat tcgttcataa tagcgcaatg tatcggccga
     2521 aatgcccttt tcttcactga cttgtttgat attcatctga tcactctttc tcttaatttc
     2581 ttgccacttt acttatgtat tttatttatt ctacaacttt aagtgtactc caagtcaata
     2641 ctcatttcta ttaaaactaa gctggattat ctatatcggt tgtttgtgta ttatttcaaa
     2701 taaaaaagtc ttgttttttt attctttttt ggatatactt ttaacaattt agggaaattt
     2761 taggaaaagg ggtataaaaa tgacaacgta tcagcgaaag gtattattat cgacatcggc
     2821 gggaattgct ttagagaata tggatattat gtttttagct ttttcattat cttcaatgat
     2881 tacttctttt catattagtg gtacgcaagc gggtttcatt tcaacgatca ctaatttggg
     2941 gatgctcatt ggaggtattt tttttggcat catagctgat aaatatggac gtgtgaaagt
     3001 attttctcaa acggtccttc tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa
     3061 catctacttg gtttatctct ttcgctttat cgcaggtgta ggagcgggag gagaatacgg
     3121 ggcgtgtatg tccttgattt ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc
     3181 ggttgctggt attggtgcgc aggtcggtgc ggctttggct gcgattttag ctgctgtgat
     3241 cattccaacg tttggttgga agatgttgta tgtcgtaggt gtactgccgg tgttgatggt
     3301 cttgttcatt cgtcgcggct tgactgaacc aaaggaattc aaatctgtac aatctgccga
     3361 aaagaaaacc aaacttagca gactgtttga atcaaaacat cttgcttggc aaacatgtgg
     3421 ccttagtatc atggtaactg tccaaattgc cggctatttt gggttaatga attggctgcc
     3481 ttcaattatg caaaatcgat tagggccttc ggtttctggg tcttcattgt ggatggtcag
     3541 tactatttta ggcatgtctt tggggatgat gacttttggc ataatcatgg ataaattggg
     3601 gcctaaacta gccttttctt tgtttttaat ttgttcatcc ttatctgtct atctacttgt
     3661 gttagcccat agccagtgga gtttggtagc agccgctgtg atagtcggct actttatcaa
     3721 tggcatgtat ggtggatatg gcgcagtgat tagtagtttg tatccaacag aaatccgcgc
     3781 gacggcgaat aatttcatta tgaaccttgg acgagctgtt ggtggttttt cttctatcgt
     3841 catcggtttc ttgatggatc attatagttt aactgccgta atcttgtttt tgagcggtat
     3901 ttacctagtc agtctgctgg tcttgacaac tcttactggt gttacagcat taagggaaag
     3961 cttgcagcat tgatagttga gtctgtaaat gacaagaaag aggtgtggca aaagcttgca
     4021 tctgcggtaa taagaccagc caaatcaaaa acatgagaca ctgtttccgt ttggctggtc
     4081 gtattacttg atgcagggca gctttttcaa ctgctaattt ccttctatct ggcggcgact
     4141 attctttgac gtccgtcatt tcaaagtact ttactaataa cttagcataa cgaaaaactg
     4201 tggatacact cgtatattga gcgtctgcat aatcttccaa atttttctca ggatgttgat
     4261 acatgaattc aatcaaatga taattatatg attttctgta gaaatgaaga taaataatgt
     4321 agattgggaa gttctcctgc ttttccatag aaaagcgccg attattctca ttcttcaaaa
     4381 tgaccttatc aggatatttt tggaacaatt cttctaaatc tgttagataa ttcaagatag
     4441 acttacttga taccttcaat tcgttttgta gttcttctat tgaacaccaa cgttctgttt
     4501 caagcagtaa atgaatcatt ttaattttct tttgagaaac ttttgttaaa aaagtctgca
     4561 tattttctcc tcctgtatct actttactat actacataca agaaaaaaaa cggatacaaa
     4621 tatcatattg tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttccta
     4681 agtattctat tgatatgaat catttaatcg tttattatat aaatgtcaat aatctaataa
     4741 taaaataacg tacatatttt attattgcat acttaataac agaactatag caaaaaatcc
     4801 cgtgtatcaa cacgggatta gattaagttt cctcaatcac tattcataac acaattatta
     4861 taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag
     4921 gaggaaattt agcgaaaaaa ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg
     4981 ggtgtggtgc tactacgtct actactcaaa gcacaagttc agatcaggct tccagcagca
     5041 gtaccacgat ctccgaaagt acggcagatt catcgaaaga acaagaacaa attgaaaaga
     5101 aaaaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga
     5161 atctgctttg tcacaagctg aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc
     5221 agctatccaa gcgctacctg aaaacaaaca agagttgaca agccgtcttg ctgcaattga
     5281 ttccacaatc aaagcaaaag aagcagaaga agaacggaaa aagcaagaag aaattgcacg
     5341 acaacaagca gaacaagcgc aacaagaagc aatcgcacag caacaagcag aacaggcgag
     5401 gcaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaatcag cagctgaaca
     5461 ggcctctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
     5521 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
     5581 cactttaagc gaagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
     5641 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagatggc
     5701 gttccagata gtaaaccaaa aataacagtt tacacgtcca tttcgtcagc ttctctcttt
     5761 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     5821 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     5881 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     5941 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     6001 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     6061 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     6121 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     6181 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     6241 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
     6301 atgtgtattg accatataag gagtacaggt aagcaaagtc acgagatctc tgcctttttg
     6361 gatacccaaa gaatctactt catcaggtaa gaccacttgg attttttcta cttgatacgc
     6421 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
     6481 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
     6541 cccgccagtc ggataagaac tttcttccaa taatgacgtt cctttttgca aaaatatttc
     6601 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
     6661 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
     6721 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
     6781 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
     6841 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
     6901 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
     6961 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag gttgtgaaat
     7021 cagcgccttg acctgagtat tagcgaagca aacggcaaaa tagttcctcc tattttgccg
     7081 tttgttgagc gaataccaag agaccgacca cttgtcacaa cctttctccc catttgaatc
     7141 actatcgttt atttatcgta tgatttttgt ttccaatagt ttaagcttct gtttcttgat
     7201 ggcgtttttt gaaatagatc actgctcctc caactaaagc agtacctacc aagatgaata
     7261 cgatgatccc cataccacca gttgaaggca agattccttt aggttggtta tcgatcgttg
     7321 tttcatcaga agttgttgga tcatcatcta ctgtaaattc agtgtacgca gcttcattct
     7381 taggtaatgc gtagccttct ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca
     7441 gcccttcgat tgaaaatttc ccttctgaat cagaaatcaa tttcacttct tcccagtcag
     7501 aacctgtgac ttcatctgac caagcatatg ggtattctcc tgaggcaagg tttgctattt
     7561 ttttcattaa tttaacagtt ggatcttttg ctcttagaat agaaaattct gcatcggcta
     7621 atttttcttg cgtgttcaca tcttgtttga tgaagtggaa atcaccgtag ttttggacat
     7681 tttctacagt aatcatgtta tccgtagttg tattttcccg attgactaca ccgtttggat
     7741 tcccaagatt ggcttcatct gcgccatgga tgataccggt tgtttgttgc aattttccgt
     7801 tttttactat aaaagtagtt tctttttgtg gattggttaa ttgataacca tctggtgctt
     7861 ttgtttcaac taaagtataa ttgccgtcta ttaatccatt gacactgatg gatccttcag
     7921 tatcagttgt gaatgtcaac gctgactcag caggatcata gagagcgaca ccagtgtttt
     7981 catcaaaacc ggtcacgaat tgattctctg aatttttgat ctggaattgc gcccctgcta
     8041 acttatctac ccctgaaaag ttcccagctt tctggatttt gatattgctt tctttgatga
     8101 catttttagc atataggtga atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa
     8161 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
     8221 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
     8281 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
     8341 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
     8401 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
     8461 attaaccgta acattttctg gtgtaggtga tacggctgca gaaacctctg cagttcctag
     8521 gccgagaacg aaaggaagca gcatgatagc agttcctaaa aacgtccata tttttgtttt
     8581 gatgtttttc attttatttc cctcctattg tttccttttt gttgtttttt ataaaaccaa
     8641 gctgtccctg ttgaaaggac tagcaataac cctaaaattg agaaacctcc aagcatctct
     8701 tcgcttgttt ttggaaagct tcctccattt ttttcatttg atggaccatt tgtctgcatc
     8761 tgaactggcg tttttccatt attcgtcgga tttgcttttg gttcgttgat gatttccaat
     8821 ggtataggat gaagcggatc ataactgcct ttttcaactt caaagagaat ggttgattga
     8881 ctcaatatat atcctttagg cggtttaatc tccactagtt cgtaacttcc aaaagacaat
     8941 ccatggacag aaaatgttcc atctttactg gaagtaaaaa tcgttgctct tgaatcatct
     9001 ttggctaccc aatgatttat cccgttttct tgcgctaagt attggtcatt ttttttgact
     9061 agaaattgag catctgctag tttttgtttc gtttgccgat ccactttgac aaattgtttg
     9121 cctcctgttt ggatggcaag atgtgtttct actgtttgat tgtcagtcgt caaacgtaca
     9181 tcgttatcaa atgtcgtttt ttcactgttt tctttcaatg tcatttggta acggatcgtt
     9241 aatttttttc cagcaaacga actaagatct tttccagaat ctgataagac aaaaccatgt
     9301 tttatttttt gtgtgtgaaa tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt
     9361 tctggttcta gccataactg aggatcagct gtatctttga tcgtataggt tcctatctcg
     9421 ttgatgttct caggaattgt tacggtgatg agataaggga ctttatcccc atctgcaaaa
     9481 tctgtctgct tattctctac tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg
     9541 ctctttggat acaagtggat ggtcgtcaat ttatgattag atgaatctgt cacaggaagc
     9601 acaacgacaa atggtgctga tttttctttt acttaatctg aagtctttat ttccgtaaat
     9661 cggtaaacag cgtcacgacc ttgctcatct ttgtccgatg cactaaaaga agcaatgccc
     9721 tcttcctcgt ctactgtttt tgtcacattt tctgccagta ttttttcact atcacttttt
     9781 tgtgctagtt ttctttgtgc gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat
     9841 tcatccgaga catcatatat agcgaaagta acgccgttca acccttttac tccttgaaag
     9901 ttatccggga tatttattcc gtcgttttga ctgatacttg gcatcttatc gctttcgaac
     9961 attcgattat gcaaagtaaa tgtaataggt gattctctag ctgacacagt cggagaacct
    10021 ataaaaaaga ccagtaggat aaaaaagaga acagcaaata tatccacacg tacatttttt
    10081 gtttttttca tcgctgttca cctgcttttt tcttacgatg gataaaataa aagaagccag
    10141 aaaaaataaa gatcagtcct aaaaagacaa agggcagtag acctatccca cctgtcgaag
    10201 gaagcaagag agtcggattg atcgtcaact tagcggtatc tgttgcgata atcgtataat
    10261 tattgtttac acttccttcg cctaaactaa tcgttactat ttgtgatcca atatctgtcc
    10321 ggctgttaac tgttagggta taatccaatg attcgcctaa gacaacaccg gtaacgtgga
    10381 gatttccaac cgaaatcgag tgactttggg catcataatt aaaggcagcg tcatcgacag
    10441 taatcactgc gttagcttta tagatggtat aagttcctga tgtgttggct atatcagtaa
    10501 atgacaactt gggatttgct gctttgactt tgtctattcc gctattcgtt agtttaattg
    10561 tatagctgcc agcattaacc atattttttg ctgattgtgc atctgctgtg taatccatac
    10621 tgctaagtgc cacttgtact ggagtaagcg aactagtgac accctctgct agcttgacat
    10681 ggattgacgg gacacctgct tttccatcat atgtcttact gccactgagc tctaccgcac
    10741 cgctgatttg cttaatcgta tagacatcag atgtctgcac gatagtaaat aaatagtttg
    10801 ttctggcagc tagtttagct agtccgcttt ttgtcaattg ccaagacgta taggttccaa
    10861 tttcagtagg cgcagttact tcaactccct tttcattcat cgctttgaaa tcagttggat
    10921 tccatgtgat ttgatcaata ctgagatcat tgtttaaaag ggatgttgat tctgttttat
    10981 cattaaaggc atatttaacg atcggtacaa cagaaagacc atcatagtac ttactgccat
    11041 ttgcattgat tgatactgtt gcatttgtct tataatatag gatgacttca gaaatctgtt
    11101 ttccatttgc gtcctttacg attacatttc catttgtatc taaactagct aaaacactca
    11161 ttgctgattg gttctttgtt tctatcgctg aaacagctgt aaaggtatat ccgttgatag
    11221 taggcgtatt agtcgtcagg tcttgtgcag cacttccgta agtaagattc attattttag
    11281 aaggagcaat catctgtcca aaatagtctt gatacttaat agtgattggt gtttgtttgc
    11341 ctgtataggt caccgtatcg acgttacttt ctagggctgt gaaagaaccg tttgtttgtg
    11401 caaaagaata gcccgttact gctgttggag tgataaccac tttgtcaccc acatggattc
    11461 cagataatgt agtcccgtac gttccgattt ttgctgctcc accattgact gtataagaaa
    11521 aagttcctgt ccgattcgca ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg
    11581 aaacatttcc cttaccatca acgatctgcc catttttgat cgagtacccc gtgaatggaa
    11641 tatcatttgc taactgtata ccgattgaaa aagtactcat acctcctccg gcaggaagcc
    11701 ctccccatgt tgagggtgta aaagaataac ttttatccgc attgggtgta actgaatcga
    11761 tcgtatttcc agcggtagcc acccccatat agatactttt tgggaatgtg atagtagtac
    11821 catttactgt ggagccaata aacagattat ggatatctgg acggttatca ataaaagcga
    11881 ttgctttggt tacgccttgt aacgcagttt tatccgcact catcgatccg ttaaccgcaa
    11941 caatgactcc atctttggca tatatcactg tatatccatc tggtatggta tgtccgttga
    12001 aattcgcagt ggccgcaaca gtactaaatg tctggcttct acctgtttct gaataagctt
    12061 tctcccctgc tgcttttgct gctgtgattt ctttttctgt cggatcaatc agttgagtcg
    12121 aggatgtggt tgaggttgtc tcgttgttgc tagactgatt ggtgctttct atggactgta
    12181 tttcagaata gggtggacta tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt
    12241 ctgtataaac gtttacataa atacttgata agctccctaa gaaagtaaaa atcaagatga
    12301 tcgaaatcat aatgggatgt aagtattttc ttttagatac agctttgagt ctagaaacaa
    12361 aatgtttttg acatatacgt tggattttat tttgagtttt cttaatcatt ttcatccctc
    12421 ctctcaataa aaaatcatca aataatcctt tacacgttat tctaaattta atttttatta
    12481 ttttattgat gtatagaaag tttggggctt tttttctctt tttattctaa ggttatttta
    12541 gtcttgtcct ttaatacaaa gattttattt ttaaataaaa aatactcttt ttttacgatt
    12601 attgaaaaaa aaagataaaa cttgattaat tgaagtattg cttaaatttg gaagctatga
    12661 gggaagaata aaagagttag gacgggatta tctactattt ttctttgttt taaatatttg
    12721 ttatctttga acataaaaga gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat
    12781 aaacggtgtt caattagctg atcttgtatt cacttaaaaa atggtaaagt acgagttgct
    12841 attccccatt ttcaattttc taacgctcag gacaaaatac tgttttcaca acctcattgg
    12901 aataaaagca tatatattgt ttcaagaacg aaatcttgtt atctttttaa agggcatctg
    12961 tccaaaatct aattttagga ggtacgtttt ggtatttatt cgtacaaata aaagagaatt
    13021 atctttattc atgtaacgca tccaattgat tttttcatgt acgctcctgg aacgatttta
    13081 ctgacgattg cttatttaac tgcgaatcgc tcattagctt ttatcatagc tatccatatt
    13141 ttgaataacg ttcttggctt tgtattatag atacagatga caaaaaaagt tcaagtatgc
    13201 ggtgtagaac caatatcgca tacttgaact tttttcaatg agtataaatt aaaacttcta
    13261 attttcaatt cccatcatct gatatgcccg accgtctgaa cttcttttga aaaagccaaa
    13321 tgtgattaga taacgtcttt gttcgacata atcgccacta gaaaatacgt tcagcagtat
    13381 cgaattaacc tctgtttctg tataagtcac tccatggtca aagcgtttca agacttcttt
    13441 caataggatg tatttctttt ttgttgaact tggtacttgt tttacttttc catcattcat
    13501 gaatgtgtct aaaactttca tcttttcttg ctccgtataa gtataaacca tcttctttct
    13561 ccttttgatt ctaagaatac tccatttatg ttagaatatt ctggattttt tattaaagcg
    13621 gttactctta agaaagatat tatcatagtg ctcttttttt tactactact tataggtaga
    13681 tttattctaa tccattattc tcccctacca ataattaaag aaaaaactgg atcagggtgt
    13741 tcaaattaca tgctaagaaa aaagaaccct tgaataccaa gggttctttc tcagtctgat
    13801 aacaatgatt aacgacggat ttctttgata cttgtgaaaa gtcgttaaag tattgatata
    13861 aaaggatttt tattacctct gactacatat tgactaattt gttaaaaaat cgtttttcaa
    13921 gaatcctgta ataccagtgc tttcaccgtt tttttaactc tctaaagaac taaaagcaat
    13981 cagaggtttt accttttcat ttgacgttaa gtggcgataa attttcgaag ttgtttggtc
    14041 agtatggcct aagcgatctt tcaagacttc ccagctacag tgttccgcta ctaagcttgc
    14101 cattgtatga cgagctaaat gagtatgaaa tggcttatta attcctgagc gattcacgca
    14161 ccgtttgacg tgatcattta catactctcg acgataagga acaccacgtt tactagaatc
    14221 aagatacaca aacaaatatg tttcttctat aataaacctg taatgttgcc tcatctcacc
    14281 caaagcctta atcaactgtt gtagcttttc cataactaga ggagttacta gaactcgtct
    14341 agcagaacta actgtcttag tagtttcaat tcgaaaatcc ttactcttac ctttaactgc
    14401 cgtttgctta tagaacagaa tttcatttct tttgaaatct atatctccag gctgtagagc
    14461 aagcgcctcg ctgactcggc agccagtgta aaatagaaca tggaaaagta cagacatcgg
    14521 taattcctca tgaacaatgc cataattcaa aaaagttctc aattcatcga cagtaagaaa
    14581 cttgtcctca atattattta tggcttcttt taaatcagtt accgttaact gatattttgg
    14641 cattctcaca gacttcattg gattttcact tatcaaattc tttctttgac aatatttaaa
    14701 aatcatattt agaacttgaa taagtgattg taaactattt ttggcatagc caacatttgt
    14761 tccattaacg ccacattcct ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa
    14821 atcagacaac tttttatcac caatccatcg aacaataatt tttaattgtt cctctcgctt
    14881 atcataggtt atttcctttt gttcatttaa cgaacggtaa tattggaacc attgctcacc
    14941 tacaacacta aacttgtcat tgcttgcaac aatttttttc ttaagccttt gttgtttctc
    15001 catttccaga taagcgagtt gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg
    15061 atatgcttga aagtaccatg tgatcctacc agtagaactg atttgcttct tgaatcgatc
    15121 tgttttttcc atttattgat tcctcctatt ttgaaggaac ccaacaagca gaaacccttt
    15181 ggaaatcttt ctatctatgt catcattgta ccgcatctta gaaagaaaat gtaacttata
    15241 aagatcgaag tagtcgcaat ttttctcgat agtcattcga atcactcttt ctcatccatt
    15301 ctaaaacatc atcacgaaaa tagtgtcctc gaatcccgta ccaacaggga ggaaactcac
    15361 caagttttac taaacggttc agtgtactat cacttatctg aaatagtcgc tttaaatcag
    15421 tatttgttaa taccagaggt aaatgactga ctgcaagcaa tttttccgtg taatcttcca
    15481 gttttttttg taaaattgaa tagaactcta tttccagaga attaacattt gttttaactt
    15541 ccaactccat cacccttctc tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt
    15601 gtaaaaaaac actccagtac tccatcaaaa cggactagaa aagaggaatt tttaatatga
    15661 aacaaggaat tgcagacata aaaataatta aagaaattct tgaaaaatct acggctaatg
    15721 caatagcatt cggaacagga atcaacttga gtacagtaaa aaaacttaaa agtggagaga
    15781 gagctgaaga aaaattaaat cttgccgatg ctattaaaat cacagaattt ggcatgaaaa
    15841 atatgccaac caaaatcgaa atatggaaat agtagttttt ctgatctatc agctgttttg
    15901 ccaaagcaca taggaatctc acctcccccc agtaatggcg ggccgatttg gttacggaag
    15961 tatcattatc gtagtcgcga ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc
    16021 atggcgtaac tagagaatca aaatctacat gtactgatag atttgctatt catttgtcaa
    16081 agagcgctag gctattttca gcctatggaa atcctttatt ttgacccgat aaaaaacttc
    16141 catagaatga aaaggaaagg gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct
    16201 tctcaatcaa ttttgtttcc agttgtctac gcatatattc atctacatac ataaccattc
    16261 ctttttcttc ttcataaact ggtttcatgg ctaaccgtga aatataattt tgatattttg
    16321 acagaacgat ggacatggca attgaatcac cagaagctgc ggctttgatt gtcgttagtg
    16381 gtatgaatgg tatatatcgt ttattttgct tcatatttga cacccaaata ctttcctagc
    16441 tcttccaaag ccatttgtcg ttggtaccaa acactgccga gacttttccc aataatatcg
    16501 ccaatttcac gatcattaag accgatgagg aaggacataa aaatgatttc ttgtctttga
    16561 ctaggtaatg ctgcaatcgc atttgccaac tcctcattgg aaataaagat tttcatacca
    16621 aggacaataa aagtaccact actattcaaa tttggttcca aataatagaa ctctgtacct
    16681 aacccttttt gttccaatat ttccattgat atttgccgtt ggcgaaatct agcgtacttc
    16741 ttttgaatat ttaacgcctc attgcagact actttcttac aaaaactgtc aaacgtatgt
    16801 tgaatgtggg tttcctctga gtcgcccata atattctccc cctcaaaaaa ttcattcaac
    16861 tcgttttact tctatccctt tccattatta ctacaatcta gga
//
LOCUS       DACBZN010000055        16865 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.055, whole genome shotgun sequence.
ACCESSION   DACBZN010000055 DACBZN010000000
VERSION     DACBZN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.055"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            7..849
                     /locus_tag="IVD64_001727"
     CDS             7..849
                     /locus_tag="IVD64_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6148981.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            846..1796
                     /locus_tag="IVD64_001728"
     CDS             846..1796
                     /locus_tag="IVD64_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6148982.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1811..2044
                     /locus_tag="IVD64_001729"
     CDS             1811..2044
                     /locus_tag="IVD64_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP6148983.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2375..2959
                     /locus_tag="IVD64_001730"
     CDS             2375..2959
                     /locus_tag="IVD64_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6148984.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3090..3757
                     /locus_tag="IVD64_001731"
                     /pseudo
     CDS             3090..3757
                     /locus_tag="IVD64_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            3759..5183
                     /locus_tag="IVD64_001732"
     CDS             3759..5183
                     /locus_tag="IVD64_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6148985.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5201..5770
                     /locus_tag="IVD64_001733"
     CDS             5201..5770
                     /locus_tag="IVD64_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP6148986.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5880..7634
                     /locus_tag="IVD64_001734"
     CDS             5880..7634
                     /locus_tag="IVD64_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148987.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7588..9360
                     /locus_tag="IVD64_001735"
     CDS             7588..9360
                     /locus_tag="IVD64_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6148988.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9883..10152
                     /gene="rpsN"
                     /locus_tag="IVD64_001736"
     CDS             9883..10152
                     /gene="rpsN"
                     /locus_tag="IVD64_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6148989.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10167..10346
                     /gene="rpmF"
                     /locus_tag="IVD64_001737"
     CDS             10167..10346
                     /gene="rpmF"
                     /locus_tag="IVD64_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6148990.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10378..10755
                     /locus_tag="IVD64_001738"
     CDS             10378..10755
                     /locus_tag="IVD64_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148991.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10776..10925
                     /gene="rpmG"
                     /locus_tag="IVD64_001739"
     CDS             10776..10925
                     /gene="rpmG"
                     /locus_tag="IVD64_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6148992.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10949..11932
                     /locus_tag="IVD64_001740"
     CDS             10949..11932
                     /locus_tag="IVD64_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6148993.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11962..12084
                     /locus_tag="IVD64_001741"
     CDS             11962..12084
                     /locus_tag="IVD64_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6148994.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12102..13625
                     /locus_tag="IVD64_001742"
     CDS             12102..13625
                     /locus_tag="IVD64_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6148995.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFRDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13649..13888
                     /locus_tag="IVD64_001743"
     CDS             13649..13888
                     /locus_tag="IVD64_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6148996.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14081..14542)
                     /locus_tag="IVD64_001744"
     CDS             complement(14081..14542)
                     /locus_tag="IVD64_001744"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6148997.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14737..14955)
                     /locus_tag="IVD64_001745"
     CDS             complement(14737..14955)
                     /locus_tag="IVD64_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6148998.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15073..16689
                     /locus_tag="IVD64_001746"
     CDS             15073..16689
                     /locus_tag="IVD64_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6148999.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
BASE COUNT         5900 a         2339 c         3190 g         5436 t
ORIGIN      
        1 aataatatgc ttcaaaattt tattaatggt ctgtatgact ttcacttttt acagaatgct
       61 ctaattacat ctgtagttat agggattgtt tctggaacag taggttgttt tattatttta
      121 agaggaatgt ctcttatggg tgatgctatt tctcatgctg ttttgccagg agtagcaatt
      181 tcttatattt taggtattaa tttttttatt ggtgcgattg tatttggtct attagcttct
      241 atcattatta cttttataaa aagcaatagt gtaataaaag gagatacagc aattggtatt
      301 actttcagtt cttttttagc attaggagtt atccttatag gagttgcaaa tagctcaacg
      361 gatttatttc atatcttatt tggaaatatt ttagctgtac aagatattga taagtggatt
      421 acaattggcg tttctgtttt agtgttggtt acagttgttc tattttttaa agaattatta
      481 atcacttctt ttgatccatt aatggcaaaa gcaatgggga tgaatgtcag tttttatcat
      541 tatctattaa tgattctatt aactttagta gcagtaacag ctatgcaaag tgtgggaact
      601 atattaattg ttgcaatgtt aattacacca gctgctacag catatttata tgcaaacagc
      661 ttaaaaacta tgatatttat ttcggcgaca gtcggagcgt gctcatcatt gctaggtttg
      721 ttcattggtt atagttttaa catagcagca ggatcaagta ttgttttaac agcggcattc
      781 ttatttgtaa ttagttttct aatctcacca aaacaaaaat ttttaagaaa gaaggagaga
      841 aagttatgat gagaaaatgg aaagcagtac tgggaagtct ggggattttg attgctcttt
      901 ttatattcgg ggcatgttca acaaatagta aagacaaaga tacagtggct tcgaacgaaa
      961 aattaaaggt agtagttact aattcgattt tagcagatat tactgaaaat atagcaaaag
     1021 ataaaattga tttacacagt atcgtaccta ttgggaaaga tccccacgaa tatgaacctt
     1081 tgcctgaaga tgttcaaaaa acttcaaaag cagatttgat tttttataac ggtgttaact
     1141 tggaaactgg aggaaatgct tggtttacaa aattagtaaa aaatgcgaac aaagaggaaa
     1201 acaaagacta ttttgcagca agtgatggca tagatgttat ttacttagag ggtcagagtg
     1261 agaaagggaa ggaagatccc catgcttggt taaatttaga aaacggtatt atttacgcta
     1321 aaaatattga aaaacagtta gcggaaaaag atcctgataa taaaaaattc tataaagaaa
     1381 atctagataa gtatattgaa aaattggatt ctctagacaa agaagctaaa tctaaatttg
     1441 cttcaattcc gaatgataaa aaaatgattg ttacaagtga aggatgcttt aaatatttct
     1501 cgaaagcgta taatgtgcct tctgcttaca tttgggaaat caacactgaa gaagaaggaa
     1561 caccagatca aataaaacac ttagttgaaa aattacgcac aacaaaagtt ccctccttat
     1621 tcgtagaaag cagtgtggac gatagaccaa tgaaaacagt atcaaaagat accaatattc
     1681 ctatctattc aacgattttt actgattcaa ttgcagaaaa aggacaagat ggtgatagtt
     1741 actatgcgat gatgaaatgg aacctggata aaattgctga aggcctttcg aaataatttg
     1801 gagggtaggt atggtacaaa ttaaaacgac tactatacta atttatttac tagcaattca
     1861 ggaattatca aaaagaaaaa agaaattaaa aaataatgat ttagcaaaag ttctaagtgt
     1921 tagtccagca tctgtaagtg agatgctggc taaacttaga tatgaggact atttagatat
     1981 aggatttcaa ttaactgata aagggaaaaa atttattgac gattacaaaa aaggttttaa
     2041 gtaaaagact ttatttttag ggaggataag aaaatggcta ttggagtgat tatgatagga
     2101 cttaccctaa tagctggata taattttttt aaatctttct cggattattt tagaaaaggt
     2161 gaatgattta gatgagtcta ttaattcttg taggattgct ttttgtatca gttattttgc
     2221 tctttctcat tagtttagct ctaaggaatg agttcagtaa tagacgttaa cgattgaaac
     2281 ttcaagtcct gtgtaagata atatagttaa ttcatcttat taggaggaag tacccataga
     2341 aaccagagat tatattacaa ctcaccaaaa attaatagaa agtggactaa gccattttga
     2401 aaaagaagga tttcagcaaa ctaagataag gcaactgtgc ttgaccgcag gcgtaactac
     2461 gggtgctttt tataaacatt ttacttctaa ggaagcgtta tttgaagaaa ttattcagcc
     2521 gcatattaat gcgcttatta atatgtataa tgaagagttt accaaatacc tcttaaactt
     2581 agaaaatata gatatagaga aaagttggag agagaacttc aatgaattag atccatttat
     2641 taattatatt tttgagaata aacgtatatt cgatttggtt ttattccaat cagctggaac
     2701 caaatatcaa gatattgttg aaaaaattac atcatttgtg acaaaaaaga cagtctgttg
     2761 tctagataag ttaaagacta tcggaatgat caatcaaaat ttagtcttta atgaagaaga
     2821 aatccacttc tacatttact cattctacgc tacattctat gatatattga agcatagcta
     2881 ttcaaaagaa aagactatgc tattaaccag aagattgtat caattttctg ttcctgggtg
     2941 gattgaatta ctgagttaat taaaaaaaag cttgcattga gaattgttct cagttatgct
     3001 ttttgataag atcgcactgt tatctgaata acggtgcatt tttgtcagct taagataaca
     3061 gtgcgatctt attttttgga ggtgaaagag tgtcaaaaat caatccgtta accttgatta
     3121 ctttaaatat ttttttccgg tcattatttt tttaggaaag ggaaatatat ttcaagtggg
     3181 gtgtttcatt attgctacag ttttagtatt gatgtttcgt aaaataaaga catgtattta
     3241 tttcatcttg atttattcag ttctgttttt agtaatgaaa attattgatg ctatcaagat
     3301 agagtttttt actatatttt ttgggacatc cttatatatt gtatttcgaa tgttgccggt
     3361 cgtaatgatc tcatggttgt tggttagtgt ctatactagc aatgaattat tatcctcttt
     3421 agaaaaaata catttaccga aaaaaataat gttaagtatt acagtagtga tcagattctt
     3481 tccgacttat cgttctgaaa taagaatgat taaagaatct ttaaaaatga gaaacattcg
     3541 cttgtctttt tttcagcctc tgcaatattt ggaatttctg ttagttcccg ttcttatacg
     3601 tgcaactatg attgctgaag aaatgaccgc taatgcaata acaaagggga tagaatctcc
     3661 tgtgaaaaga agttcttact ataaaacaaa catgagctta ttagactata tcatcctgtt
     3721 ttccgttact atttttttct taagtatttt gatttgaggt gaaagaattg tttgaattta
     3781 acaatgtttc tttttcatat aaggatgtta aggaacaagc gcttgttgac cttaattttt
     3841 caataaaaaa aggcagtctt acattgatta ctggttcttc tgggagtggg aaatccacac
     3901 tattaaagtt aatgaatgga cttattccaa gactttatga cggaaaattg gttggggaaa
     3961 ttttatttga aaataactcg ctacaatcct taacacaaga aagtatatca aaaaatattg
     4021 gctacgtaag tcaaaatcct agaggacaat tctttacaac aaatacaacg agtgagttag
     4081 tattttcgat ggaaaacttt ggatttacaa aagagcatat ggataaaaga atcaatgaat
     4141 tagctgaatt attagatctt tcaaatatat tagataaaaa cattttttcg ctatcaagtg
     4201 gtgaacggca gaaaatttct attggatgta gtttatcgct aaactctaaa ttaattatat
     4261 tggatgaacc gtcttcaaat ttggacttca aagcgactaa gaaacttgct gacttacttt
     4321 gtgatttaaa aaataaagga tttactattg ttatagctga acatcgtttt tactatttga
     4381 aagaattaat tgatcaagtt ctatttgtta aaaatagaac tgtaaaaact acaactattc
     4441 agttgttaaa aactgaaaac aataacgcat taaggaaatt tgacattttt aatagttcga
     4501 ttgaagttcc ctatatcgaa aaggtaagtg cagatgtatt aaaagttgaa gaaattactt
     4561 atgaaaatat tctaaaagac ataaattttg atgtcaaagt tggagatgtt attggtttgg
     4621 tagggaaaaa cggtgtgggg aaaacaacct tactaagaat actcatgaag attattcgcc
     4681 ctaccaaagg aaagataatc gaaaataaaa aaaatttgtc atcgccgttt ttagtaatgc
     4741 aagaaatgga ttaccaattt ttcacagaat cagtgaggag cgagttagcc ctggggaatg
     4801 aaatagttag taaagatcag caagaaaaaa ttttaaaaaa aatggaactt tataaaatga
     4861 aagatcaatt accgtttgat ttatccggtg gagaaaaaca aagattattg gtttctatag
     4921 cttcattgtc aaaaactaat ttatttctat ttgatgaacc tacaagtggg ttggattata
     4981 tcaatatgga aaggattgct gaccttatta aagacttaaa agaaaagggt gcagtagtta
     5041 tagccactca tgatccggaa tttctttata aaacatgcaa tagaattatt tatttaaaaa
     5101 atggtggaat aaaaaaagat ataaaactta gagtagaaga ttcaactttg atagaacaga
     5161 tttttaatga cataacgaaa tagaattgag gagaatatat atgaaaacaa aagattatat
     5221 ttttattggt ttagctacag ttatttccct agtaatttac tttttttcaa taatggttag
     5281 tagtattggt ggggcgtttg gtcacagcat atccccaggg atttttggat tattatctgg
     5341 aatcatattt gtttatatta gctataacta ccctagaaaa ggaattttta ctatttacac
     5401 aattgtttta ttgttatttt ttacgttaat gggcggggcg tacttacctt ggtttatcag
     5461 ttcgattagt atggcgatct tagctgatat tattctatct gtatttggat atgatcgagc
     5521 aattcctcaa gttgtgtctt gggttctaat gcaactaggc agtgctgccg gacagtggat
     5581 tcccatatgg ttctttacag atcgttttcg tcaagactgg atagatagag gacaatctgc
     5641 tgcaacaatg gatgcaatga tccactacgc ggtgggtata tggggaatta tttcagtgtt
     5701 agttgtagca agtttatcta tgattggtgt attgattgga agaaaagtct taaaaaaata
     5761 caaaaaatag aacgtagttt accttctatc tgtttgtaca tctctaattt acgctattac
     5821 ggaaagcatt aacagttgta aacgaataag agaagaaatt gaatgcgggg gaggaaaaaa
     5881 tgaaagtata caaaaaatta tttagttatg tccctaaaat gaaatactac ggatggttag
     5941 ccactttatt ttctgatttt tctgtcgtat taacagtcta tggatattac agtatttaca
     6001 aatttcttca gtctctaatt attgtaggag atgaatattt agctaagagt tatgcggttc
     6061 aaactgttgc ttggttaact ttaggagctt tattttatat tttttctggc ttattttctc
     6121 atatccttgg gtttcgctta gagactaatt tgagaaaaaa gggaattagc ggtttaacag
     6181 aagcaagttt tcgttttttt gatttaaatt cttcgggata tattcgaaag acaattgatg
     6241 acaacgcggc taaaacacat atggcaattg ctcacttgat tccagatagt gctcaagcca
     6301 tagtaacacc tgttttatca gttgccttag gctttttcat taactttaaa attggattgg
     6361 ttctaatcgc catgttagta attggtgtat tcttgttaaa gaaaatgaca gagaatacaa
     6421 attttattca aaaatatcaa gaatcgttag atgtgttaag ctctgaaacg gtagaatata
     6481 tccgcggaat tcaagttata aagatatttg gtgctaaagt aacatcattt aaagccttga
     6541 ataaagcaat taacgattat gctaagtttg catataagta ttctttgagt gggaaaacac
     6601 cttttgttct ttttcaatgg ctattttttg gcatagtttg cattttgata gttcctataa
     6661 ccttttttat aacttctctt accactccca agatcttagc tgttgacttg ttgatgttgt
     6721 tttttttaag tggaattatt tttgtttctt tcatgagaat aatgtatgta agtatgcact
     6781 tgtttgaagc aagatatgca gtcgaaacac tagactccct ttatttagaa atgaagcaag
     6841 accaactaaa atatgggaac gagacacaga ttaaaaattg tgacattgca tttgaaaatg
     6901 tcagcttttc atacaaagaa gagtgtgttt tagaaaattt caacttagtt ttagaacaaa
     6961 aaagaattta tgcattagta ggaaaatcag gaagcggaaa gagtacgata gctaaactaa
     7021 tttcaggttt ctacaatgtg gataagggac agattaaaat tggtggtaag cctttaaatc
     7081 actattcaaa agacacaatt attaacacaa ttgcttttgt ctttcaagat acgaaactat
     7141 tcaatcagag tatttacgat aacgtttcta tagcaaatcc tcaagcgcca gcaagtaaag
     7201 tatttgacgc gctaagatta gccggttgcg aatcgatttt agaaaaattt cccgaaagag
     7261 agcatacctt aattggtagt aaaggagttt atctgtcagg aggagaaaaa caacgtatag
     7321 ctattgccag agcaatttta aaagatgcaa agattgttat tatggatgaa gccagtgcat
     7381 ctattgatcc agataatgag tatgaactac aaaaggcttt tcaatatttg atgaaagaaa
     7441 aaacagttat tatgatagct catcgattat ccagcattaa atctgtagat gaaattcttg
     7501 tcttgtcaga aggaaaaata gtagaacgtg gaaatcataa tgaattaatg gctaaacatc
     7561 gagtgtataa acaattggtc actaattatg agaatgcaaa tgattggagg cttgacaatg
     7621 aaaaattttt ataaaaaaaa atttgcttta actgatcagg gcgcagaagc tttaactaaa
     7681 gcttctattt ctagtttttt tgtatactgc attaatatgg taccagcatt cattataatg
     7741 atgctgatag acgaattagt tttagaaaat gcaaagcccc gttggcttta ttttgcagta
     7801 tcgtttgtta ctttgctttt catgtattgg ttgttagata gggagtatga aaacttatat
     7861 aatagtactt ataaagaaag tgcgcattta agagtgcaaa ttgcagacga tttgtcaaat
     7921 ttaccattat cctatttttc aaaacataat ttatcagatt tatctcaaac tatcatgtct
     7981 gacgttgaag gtattgagca tgcgatgagt catgcaatac ctaaagccgg cggtatggct
     8041 ctgtttttcc cttttatttc agtgatgctt ttggttggta atgtcaaaat gggattagct
     8101 gttattttgc caacgttatt tagttttgtc ttaatcttgt tatcaaagaa atcccaaacg
     8161 aaagccaata ctaaatatta cgatactttg agagaaaact cggaagaatt tcaagaaact
     8221 attgaattgc agcaagagat taatagcttt aatctatcta aaaaagttca agacagactt
     8281 ttcaaaaaaa tggaagagag tgaaaggatt catttaaagg tagaattaag tactttttca
     8341 gtcatggcct tatcctctat tttctcatat gttagtttag cagtagtcat tctagtaggt
     8401 gttcacttac tgttaacggg tgaagtgact atactctacg tcgttggtta cttactagcc
     8461 gcaataaaaa taaaggattc cttcgattct atgaaagaag cagtgcttga aatattttat
     8521 ttagctccta aaatacagcg aattagagct atgaaggaaa ccagtatcca agagggatca
     8581 gatagtcctc tgaaatcttt tgacattgag ttgcgagatg tctctttctc atatgataac
     8641 aataccccaa ttttagatca tatctctttt acggctaaac aaggagaagt gactgcgtta
     8701 gtaggtgcta gtggttccgg gaaaactagt atcttgaaat tagtgtctag actgtatgat
     8761 tatgatgaag gctgtatttt aattgatggg tatgatatta aacgtgtatc tccagcttct
     8821 ttgtttagca aaatcgctat tgtttttcaa gaggtaactt tgtttaatac aagtattttg
     8881 gaaaatatac gtattggaaa tagtcaagcg tcagatgagg aagtgaaaaa agcagctcgt
     8941 ttagctaact gcgaagactt tattgaaaaa ctacctgacg gttatcatac attggtcgga
     9001 gaaaacggaa gttcactttc tggaggagag cgacaacgcc tatccatagc acgggctttt
     9061 ttgaaaaatg cgccaatttt aattttagat gaaattactg ccagtttaga tgcggaaaat
     9121 gaaaaacgta ttcaggaaag tttaaatcgt ttaatacaag ataagacagt actaattata
     9181 tcgcatcgct tgaagtctat tgaaaaggta aataaaattg tagtgatgga tcaaggaaaa
     9241 ctagttgatc aaggtactca taatgagttg tatcgaaggt cagaaatata caaaaatctc
     9301 ataaagaaaa ctaaactatc tgagaaattc gtctatgaaa aagaagctca atcacgatga
     9361 tgaaataggc tttaaattaa cctacaattt ttgggatata gttataattc tttttcaaaa
     9421 agaataattt tattaaacag catctaattt tgaatacacg aaagaatcag tatgattgaa
     9481 ttagttcact taatgtctat tcttataata taaggaataa tcaaagtttt aaaaaattgg
     9541 cgataaacca gttaaacata aaagattgaa taaaaagtta tagtttcttt catattgccg
     9601 ttttaagtag aggaaaagct acttaaaacg gtatttgtgt ttgattggac taataaaagt
     9661 gaattcttat ttttcagagc ctaaactgca tattctttta aatgatttga ttatctctat
     9721 gtaccgataa attcgttttc aaatagttat ctattcatat gagttttatt tcatgttatt
     9781 tattgttgac aagagcaact cttgttggta aagtatcagt gtcaaaacgt aacgattacg
     9841 atttgaggtg tctaaatcaa aataaaagga gtataaaggt atatggcaaa aaagtcgaaa
     9901 attgctaaag caaagaaaca aatggcgatg attgaaaagt atgcagataa acgtcaggaa
     9961 ttaaaagccg caggagatcg caccgctctc gctaaattgc caagagattc aaatccgaat
    10021 cgtctgaggc ttcgtgatca gacggatggt cgtccaagag ggtatatgcg aaagttcggt
    10081 atgtcccgta ttaagtttag agaacttgcc catcaagggt tgattcctgg agtaaaaaaa
    10141 gcaagttggt aaaaggagga aatatcatgg cagtaccagc aagaaaaaca tctaaggcga
    10201 agaaacggtt aagaagaact catcaaactg tagtaaaacc agaaatttca tttgacgaag
    10261 cgaatggtga ttatagacgt agccatcatg tatctttaaa agggtactat aatggaaaga
    10321 aagtaattaa aggagcttca aaatagttta atatataact tttaggaggg aatcaaaatg
    10381 gaaattattt acccaccttt agttgaagaa ggattaaagt attatcttga aacgacccag
    10441 caatcgctag ataaatgcac tttttatcgt tcgatggtag agagaggaat tattactgaa
    10501 acgggtttac cgacccaaca ggcaattgaa aatggattag tcaaagatta ctatgaggac
    10561 caaggcttat catttgatga ttttttaaga atctatccaa tttttgaaga gtatgacgag
    10621 gagttatttc aatgtatcga tggttattgg gagatacctg ttgatatgaa agaaaattta
    10681 gtttcacaat tggaatctgg cgagttaact tttgaagatg cacaacaaat tcaagcatat
    10741 cttgaagatc gataagtatt gaaggaggaa gatttatgcg tcaaaatatt attttggaat
    10801 gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa taaacggaat aaccctgaac
    10861 ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttt aaagaagtga
    10921 aatagctaag tggtaaaaag gtgacaaaat gggcatacca gttacaatcg tttctgggtt
    10981 tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac cctaccccag
    11041 ggaagaaatt attataattg aaaatgagtt tggtgaagtt gggattgatc acaaattatt
    11101 agttcatagc gaggaacggg tgttacaact aaataatgga tgtatgtgtt gcagtcttcg
    11161 cagtgattta ttagcaatat tgatttcttt actggaaaca atagaagaac atcaacaacc
    11221 aatttcacaa atcatcattg aaactacagg tatagcagat ccccaaccaa tcattcaaac
    11281 acttttaaca gcgcctcaac taagaggaag agtatatatc gatagtttat tgactgttat
    11341 tgatagtgac agttttgaac ttattgaaag agaaccacaa gcccttaagc aacttgtcat
    11401 ggcagatcgc atttttgtgt ctagaaaaca aaacttgaat ttatctgcac taaaaaaatt
    11461 acagagagaa atcaaaagta ttaacccttt atccgatatt cgaatgttca cacatagtga
    11521 aacgattcaa aaaaaggatt tttataattt gaaaaaattt aatcatccct tagtttttga
    11581 aagtgattca tcagaaaatc actataatgg caaatactat catcaacacc atcaagtaca
    11641 tgagtttaaa tcaattctct taacttcaga tcaagacata aaaaaagatt ttttactcca
    11701 atggattgac tggctgattt taaccaacca agggagcctg tatcgtttta aaggattgat
    11761 tgctttacaa gagcaggatt ttatagttgc catgcaagag gtaaacgaac aggttaattt
    11821 tcaatacacc actcgacaaa aagaccaaac cactattatt ctaatcggaa aaatgttgga
    11881 tgaagaaagg atcaaacaga gttttgataa attactggcg gaaagcaaat aatgataagt
    11941 agtgattggg ggtaatttct aatggagaaa acggatatat ctagtgcata tcgacgactt
    12001 aaaagcccga atattaaaac tagaaaaagg gcattaaaaa ttatcaaaga acataaacga
    12061 aataaaatga agaaattagc ataattaagg aaggaaaaag aatgaagaaa atcagtgtag
    12121 gacttattgg gatagcagct ctaggattat tgggagcttg ctccagcact aatgatgcaa
    12181 aagtatcgaa tgataaagat ggaaaattag aaattgtaac aacattctat ccaatgtatg
    12241 atttcaccaa aaacatcgtc ggcgatgaag cgaacgtgga tttaatggtt ccagcgggat
    12301 cagaacctca tgactatgag ccctcagcta aggatatggc aaaagctcat gatgctgatg
    12361 tttttgtata tcataacgaa aacatggaat catgggtacc aaaagctaaa gaaagttgga
    12421 agaaagctgg gcctaatgta gtagaaggaa caaaagacat gattttattg ccaggtagtg
    12481 aagaagaaga tcatgatcac ggagaagaag atcaccatca tgaactagat cctcatacgt
    12541 gggtttcacc caaaatggca attaaggaag tttcaaatat caaggatcag ttagttaaat
    12601 tgtatccgaa aaaagcaaaa gtatttgaga caaatgctga aaagtatttg actaaactga
    12661 aacggttgga tgctgactat accactagtc ttaaagaagc gaaacaaaaa agttttgtga
    12721 cgcaacacgc agcctttgga tatttagctt tggattatgg attgaaacaa gttcctattg
    12781 ctggactaag tcctgaagaa gagccatctt ctggtcgatt agctgaatta aaagagtatg
    12841 ttaaaaaaaa taagatcaat tatatttact ttgaaaagaa tgctaatgat aaaattgcaa
    12901 aaactttggc caacgaagct gggataaaat tggaagtact gaatcctctt gaaagtctta
    12961 caaaagaaca aatggataat ggtgaagatt atgtttcagt aatggaagac aatctaaaag
    13021 cgttagaaaa aacaactatg gttgctggtg aagaggtagt tccagaaaaa gaagcgaagg
    13081 atgaaaagac agttgctaat gggtacttta gagatgccga tgtgaaggat cgcgaattga
    13141 gtgattatac aggcgaatgg caatctgttt atccgttgtt aaaagatgga attttggatg
    13201 aagttttcga ttacaaagca aaaatcaata aagacatgac agctgccgag tacaaagatt
    13261 attatactac tggctataag acagatattg atactatcaa cattaaagac aacaccattg
    13321 attttgtagt caacggtgaa catcatcaat acacatacaa atatgttgga tacaaaattt
    13381 tgaattatga aaaaggaaac cgtggtgttc gttttaattt tgaaactgat gatgcaggtg
    13441 ctggtcgttt taaatatatt caatttagtg atcatggaat tgcaccaagt aaggcagagc
    13501 atttccatat tttctttggt ggagaaagtc aagaaaaact ctataatgaa ttgcataatt
    13561 ggcctacttt ctatccagct tcattgtcta aacatgaaat tgctcaagaa atgatggctc
    13621 attaaaattc aatttttgga ggaaatacat ggtaagaaaa gtattattgt ctatggattc
    13681 caagaatatt gatgcttatc ggcgttatcg atttgcaaag caaatgagat taaatgggag
    13741 tacattagct gatattgctg caactttaca tgttagtatt gacacagttc atagagtatt
    13801 aaaacgggat cctgattgtt gttttatcaa ggtcaatact ggaagaagta gacgtttagc
    13861 aaaacggcat aatagcttgg ccaattaatt gataatacca gagtagatca tttttccctt
    13921 atttggattt gatctgcttt ttattatgtc ttgaattgtc aaatcaagcg caacacttta
    13981 tcaaagaaga cttcataagg tgttctccaa tctaaacact ttctaggtct gttattcagt
    14041 ttttcaatca tctgatttat cgttgagtta tctaccgtag ctatctcttt cgattttggc
    14101 aagtattctc gaatgagtct attcgtattt taatttgttc attgttgcca agggcatgag
    14161 gatctggaaa ataaaagggc atattattca atgcttctgt aactgcgaaa tgattggaga
    14221 attcttttcc tctatctgga gtgatcgttt tgaccgcctc atttggaata ctgcttaaaa
    14281 gttcaatcat tttctcgctc acataagcgg ctttctttct ggggaccttc tttcctgcta
    14341 aaaggaagca tgattttctg tctgtcaaag tcactaaaca agtaccacca gttttgccag
    14401 ctacaatatc ggctttccaa tgaccaaaat cacttctatc atctgcggat tttggacggc
    14461 catggatcga atgagtaagg caaatctttc cacgagactc gacatgattt tttgtatgtc
    14521 ttgtttttcc tcgatggtgc aatgaacgga tcaggccttt attgccgtgt gagaggggtt
    14581 ctgtatcgaa aagatcattg caaattgcac gttagatcgt gataaaacta attgaaaagt
    14641 ccatatgttc atgcttcaaa cgattcgaga tttcctctgg cgaccactga aaatcgacaa
    14701 ataaatgacg aacaattgac caaactcacg gatcatttag aatgtgataa cgtccgcatt
    14761 tttttcttcg ataagcataa tttttttccg ctttggaagg tgaatagttt ttttcttgtt
    14821 tcttctcagt tcatgtaaaa tagtaaacgg gctatgctga atgtcgttgc caattatact
    14881 aatagaaaat ccttcttcgt gaagcagaaa gattctttct cgttcgagta tagtaagatg
    14941 acgatactgg ctcataatta ttttccttct aggagtgttc tcgacaaact tatactagtt
    15001 ggatatcgat tatgactcat ttttatgtgt tgcacttaaa gtgtaaattc aaggtataga
    15061 aagaggttcc ctatgaaaaa aatacctatt acaatcttaa ctggctatct tggttcagga
    15121 aaaacgatac ttctaaatca tattctgtac ggtgatcacg gaaaggaaat tgctgtcatt
    15181 gtaaatgaca taggggaagt aaatgttgat ggggtattag tcgacaagaa aggttttaaa
    15241 agaactggag aaaaacttgt ttcgatgccg aatggatgca tttgttgtac attacgcgaa
    15301 gatttgattg ttgaactaga tcaattagtg caattttctg acttgaatca aattgttatt
    15361 gaagcttcag gaatcagcaa accaattcca attattcaag caattatatt agctaaaaac
    15421 cctttagaca ttgatttgac ccaacgatta gagattggtg ccattattat agtagtggat
    15481 gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga ttcaggaaag aaaagctgaa
    15541 ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt taattgatca attcgagtgt
    15601 tgtaacatat tacttttaaa taatagtgac ttagtgtcaa ccaagacgat ttacaaaatt
    15661 aacggtctaa ttgctaagtt acagtccaat gccaaaataa ttaggacaat taaaggtgaa
    15721 gtagacttat cgaaaattct aaatacaaat ctttttaata tagatgagac ttcctattca
    15781 gtgggctggt tcaaagagtt agagcttggc tatcaaaatc atattcctga aacagaagaa
    15841 tatggtatct caagttttat ctatcgaaat cggttactcc ttgacgcaca aaaatttgcc
    15901 catcttacgg ataaaaattt tcctggcaat attacaagag caaaagggtt tctttggttt
    15961 gcccaacata gtgatttttc gacgatcttt gaaacatcgg gtagctataa aagtattgaa
    16021 tcattaaatt attgggtagc tagtttacct aaaattgagt gtcaaatgat tatacaaaaa
    16081 aatcccgaat tgaaggaagc ctggcatccg ttgtttggtg aacgcattaa taaactagtt
    16141 tttattggta tgaagatgga tcaacaatgt attaaggaac aacttgaggg ttgtttaact
    16201 gattctctta atgcagataa tttccgaaaa atttcaccat ttccatggaa aaagtctaat
    16261 atgtttagcc agatatcgtt aatgaaattt ttaagaaaag ctggtgtcaa gataggcgat
    16321 gtcataatta ttggcatttt gattattacc tcttttattc ccttatttgt attctctata
    16381 gaaaaggaga atgaaacaac tgaagatgct atcaaatatg ccatcgttcg aattgatggc
    16441 aaggaaatgg atcgcttcga tttggataag atcaatcata aactcgtgaa ttatcatcct
    16501 gcgaagggac aatataatat tgttgagatt aaagatggaa gaattcgagt taaagaagat
    16561 aatagtcctg atcaaatcgc ggttagaact ggatggatct caaaacccgg tcaaacgagt
    16621 atttgtttac cccataagtt agttattagt attgagaaaa aagaaagcac agattactac
    16681 atttactaat ctggaaatag atgaggaggt ttttaataag aaaaagatat tattattaat
    16741 aatagcaggg gctttattaa gtgtgttaag cgcttgtgga caagaaaaag aagcggtgac
    16801 aacaggcaca agtatcttta acgggacctg caatggttta gttaacagtc ggttagatta
    16861 cttat
//
LOCUS       DACBZN010000056        16820 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.056, whole genome shotgun sequence.
ACCESSION   DACBZN010000056 DACBZN010000000
VERSION     DACBZN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.056"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..456)
                     /locus_tag="IVD64_001747"
     CDS             complement(<1..456)
                     /locus_tag="IVD64_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6149000.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IVD64_001748"
     CDS             complement(561..1424)
                     /locus_tag="IVD64_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6149001.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IVD64_001749"
     CDS             complement(1620..3719)
                     /locus_tag="IVD64_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6149002.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IVD64_001750"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IVD64_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP6149003.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IVD64_001751"
     CDS             complement(5072..6913)
                     /locus_tag="IVD64_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP6149004.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRLMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IVD64_001752"
     CDS             complement(7095..9488)
                     /locus_tag="IVD64_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6149005.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IVD64_001753"
     CDS             complement(9595..10455)
                     /locus_tag="IVD64_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP6149006.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IVD64_001754"
     CDS             complement(10514..11035)
                     /locus_tag="IVD64_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP6149007.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IVD64_001755"
     CDS             11355..12074
                     /locus_tag="IVD64_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6149008.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IVD64_001756"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IVD64_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6149009.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IVD64_001757"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IVD64_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6149010.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IVD64_001758"
     CDS             16434..16790
                     /locus_tag="IVD64_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6149011.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3489 c         2947 g         5767 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcagtcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa
//
LOCUS       DACBZN010000057        16700 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.057, whole genome shotgun sequence.
ACCESSION   DACBZN010000057 DACBZN010000000
VERSION     DACBZN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.057"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            176..403
                     /locus_tag="IVD64_001759"
     CDS             176..403
                     /locus_tag="IVD64_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP6149012.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            419..1585
                     /locus_tag="IVD64_001760"
     CDS             419..1585
                     /locus_tag="IVD64_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP6149013.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1820..2863
                     /gene="hrcA"
                     /locus_tag="IVD64_001761"
     CDS             1820..2863
                     /gene="hrcA"
                     /locus_tag="IVD64_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP6149014.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2957..3520
                     /gene="grpE"
                     /locus_tag="IVD64_001762"
     CDS             2957..3520
                     /gene="grpE"
                     /locus_tag="IVD64_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP6149015.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3571..5400
                     /gene="dnaK"
                     /locus_tag="IVD64_001763"
     CDS             3571..5400
                     /gene="dnaK"
                     /locus_tag="IVD64_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP6149016.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGKRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5551..6717
                     /gene="dnaJ"
                     /locus_tag="IVD64_001764"
     CDS             5551..6717
                     /gene="dnaJ"
                     /locus_tag="IVD64_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP6149017.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7085..7822)
                     /locus_tag="IVD64_001765"
     CDS             complement(7085..7822)
                     /locus_tag="IVD64_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6149018.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7824..9326)
                     /locus_tag="IVD64_001766"
     CDS             complement(7824..9326)
                     /locus_tag="IVD64_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880041.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6149019.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9585..11579
                     /gene="uvrB"
                     /locus_tag="IVD64_001767"
     CDS             9585..11579
                     /gene="uvrB"
                     /locus_tag="IVD64_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP6149020.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11589..14405
                     /gene="uvrA"
                     /locus_tag="IVD64_001768"
     CDS             11589..14405
                     /gene="uvrA"
                     /locus_tag="IVD64_001768"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP6149021.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14668..15933
                     /locus_tag="IVD64_001769"
     CDS             14668..15933
                     /locus_tag="IVD64_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HAP6149022.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYAGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15937..16668
                     /locus_tag="IVD64_001770"
     CDS             15937..16668
                     /locus_tag="IVD64_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP6149023.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
BASE COUNT         5504 a         2901 c         3661 g         4634 t
ORIGIN      
        1 tttttatgaa taaaataagg acaaaattgt ttaggaagaa tcatgagtgt aaaaaaatca
       61 tccaagaagt cgattcttgg attttttagt ggatttgttc gccaaatacg tttagtatgc
      121 gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa
      181 aatagctttt catatttttg cagattttga cttaatgccg aaggtcagct ttgcgattac
      241 cgtttatcga gaggctgtaa aagcaccgtg tagctccgat gacaataagc aagcattttt
      301 agagctaggt ttccttgatt ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg
      361 cgattttaag aaggtcgaaa acaggcacga cttttcgacg taaggagaaa gaatttcaat
      421 gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg tacattcata tcccattttg
      481 cgagcatatc tgctattact gtgactttaa taaagttttt ttagaagggc agccagtaga
      541 tgagtatgtg gaaatgctgc tgaatgaaat gagattaatg ctggaaagtc atcctacagc
      601 gaatttggaa acactttatg taggaggcgg aacaccaact tctttatctg ctaaacaact
      661 tgatcgacta ttatcaggtg ctagagagat cctgcctttt caaaatggta atgagtttac
      721 tgtagaagcg aatcctggtg atttgacccg tgaaaaactt caagtgatga aaaactatgg
      781 cgtcaatcgt ttatccatgg gagtgcagac atttgacaat cgcttattga aaaaaattgg
      841 acgtaaacat actgcagaag atgtgtatca aacaatgcaa tttttagaag cagaaaattt
      901 taccaatgta agcatcgatt tgatttatgc attaccggga caaacactag aaggatatcg
      961 ggatactttg gaaaaagctt tggctttgga tctgcctcac tattctttgt actcattgat
     1021 cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg aactgcctga
     1081 tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa agcatggacg
     1141 tcaccaatat gaaataagta actttgggct ctccggacat gaatctcaac ataacctcat
     1201 gtactggaac aatgaccact attttgggtt tggagctggt gctagcggtt atcttggaga
     1261 tattcgttac cgcaacaaag gaccgatcca acactattta cgcccattac gagcaaatga
     1321 acgcccggta ttggaagaag aaaaattgac acgaaaaaat caaatagaag aagaaatgtt
     1381 cttaggacta agaaaaaaaa cagggattac taaaaaacat ttctatgatc gctatcaaaa
     1441 aacaattgaa tcggtgtacg gtgatgtgct aagggaatta gagcaagaag ggttacttgt
     1501 taatgaagaa gaccgtgttt atttgacacc aagagggact tttttgggga acaatgtgtt
     1561 tgagcgcttt ttatttgaaa aatagtttga agaatatttg ttttttatcg aaacttgaaa
     1621 agaaaaagtt tagaaagttt gacaatgagg tattgaagta ttgatataac tggatttaca
     1681 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
     1741 gacaggagaa gggcttcttg ctatatttat aagtgaatta gcacttagaa tatatgagtg
     1801 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
     1861 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattaatgt ctgagggagt
     1921 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
     1981 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
     2041 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
     2101 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
     2161 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
     2221 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
     2281 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
     2341 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
     2401 taaacttcgt acggagattc cgatgattct tcataaatat ttccagacga cagaagggat
     2461 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gaaaaggttt atgtcagtgg
     2521 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
     2581 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
     2641 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     2701 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     2761 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     2821 acttgctgat tattaccgct cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     2881 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     2941 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     3001 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     3061 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagatagata
     3121 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     3181 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     3241 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     3301 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     3361 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     3421 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     3481 tcgcgtgtta agaccaacta tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     3541 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     3601 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     3661 gaaggaaaac gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     3721 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     3781 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     3841 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     3901 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     3961 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     4021 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     4081 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     4141 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     4201 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     4261 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     4321 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     4381 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     4441 gtacgtcaag cgttaaaaga tgcaggtctg tcacaatcag aaatcgacga agttatttta
     4501 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     4561 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     4621 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     4681 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     4741 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     4801 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     4861 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     4921 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     4981 acgatcaaat cttcttcagg tctaacagat gaagaaatcg aacgtatggt gaaagatgca
     5041 gaagcaaacg ctgaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
     5101 gacgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
     5161 gaagaagtga aaaaagcaga aacagctcgt gacgaattaa aagccgctgt tgaagcaaac
     5221 aacatcgagg aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
     5281 gttaaactat acgaacaagc tgcacaacaa caagctcaag aaaatccaga agcagctcaa
     5341 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
     5401 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
     5461 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
     5521 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
     5581 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
     5641 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
     5701 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatacga ccaatacggg
     5761 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
     5821 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
     5881 ggaggcggtc gatcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
     5941 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
     6001 gaagaaatct gtcatacttg tggtggaaac ggtgcaaaac caggcacaca accaactact
     6061 tgtcataaat gtcatggtgc aggtacgatc aatgtagaac gccaaacgcc acttggacga
     6121 gtgatgagcc gtcaaacttg tgatgtttgt catggcacag ggaaagaaat caaagaacct
     6181 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
     6241 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
     6301 aacggcggac catttggtga tttgtatgtg gtctttcgtg tagaagaaag tgatatcttt
     6361 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
     6421 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
     6481 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
     6541 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
     6601 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
     6661 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
     6721 ttctagtaaa aatataaagt agacttgccc aaattttcta tttctagtaa aaacgctaag
     6781 tcattaataa ttaaaaacca gacgaacaat catacgttcg cctggttttt ttgtagttgt
     6841 catgtttgta ccaacaaaaa agccagcttt agaataagat aatcatcata gtttctgaag
     6901 gtggtatcga ctatgtactt gatatcgtca atgaaccgat ggtactttct tatgcgatgg
     6961 aacaggtaga gcgattgctt gtttgtgcag gagaacaagc gatgcggctg gtacagttaa
     7021 gtgaaaaaac aagaaactaa aaatgcccgg tcgtcatgaa gcaaccgggc atttttggtt
     7081 tttattaaat attcaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt
     7141 caaagatttg ttgaggcgtt ccgtcttcta aaaagtttcc gtcgtcaatg aacatgacac
     7201 gattggccac ttctttagcg aagcccattt cgtgcgtaac gataaccatc gtcatacctt
     7261 gttctgctaa ctttttcatg acgttcaaga catcaccgac catttcgggg tcgagtgcag
     7321 atgtaggctc atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca
     7381 cacgctgttt ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac
     7441 caactgtctg cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca
     7501 ggtccatcgg tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt
     7561 gctggaatac cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca
     7621 agtgggcgcc gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac
     7681 gcaaaaatgt actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt
     7741 tgatggatac attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct
     7801 caactaagat tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt
     7861 gtcaatgctg tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt
     7921 tgtgttgtac gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg
     7981 gaaatgatcg ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg
     8041 atgcgtattg cttgtggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa
     8101 cttctggaag cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct
     8161 gcgatataag cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc
     8221 gtaaatccaa cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga
     8281 attcctcgga tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct
     8341 acactgaata atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt
     8401 aggagaattg ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca
     8461 agaaatgttg attcatcagc agtggaagac tcactagaag tttctgaacc atccgctaca
     8521 tattctttga taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg
     8581 aacatattaa gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca
     8641 gattcttttt cgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc
     8701 ggataatcat ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca
     8761 gcagcatcaa aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca
     8821 gcactttcgg taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg
     8881 atcgaagtat ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa
     8941 aaatcatagc ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct
     9001 tgtcctgatt ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta
     9061 aatccttgca gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc
     9121 tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct
     9181 tttttgggct ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg
     9241 tcagcttgtg caacatgacc aaacgcaagt gtcacaagtc ctgtcattaa gaaaacaaat
     9301 gagtgaaata aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt
     9361 ataggcgaaa agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga
     9421 gtttctaaca ttttatgtta taaaacgtaa catgtataat aagataaaaa agtggcgaaa
     9481 gtatgttcgg tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta
     9541 ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata
     9601 cctcccgaca ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga
     9661 ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga
     9721 ctggaacagg gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac
     9781 tgattattgc tcataataaa acattggctg gtcagctgta cggggaattc aaggaatttt
     9841 tccctaacaa tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt
     9901 atgtgccttc aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca
     9961 aattacgaca ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat
    10021 ccgtttcatg tatttttgga ttaggttcgc cttttgagta ccaaaaacaa gtagtttcga
    10081 ttcgacaggg agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat
    10141 tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg
    10201 agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg
    10261 tcgaacgtat ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg
    10321 tggcaatctt cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag
    10381 cgaatatcaa agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc
    10441 tggaagctca acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg
    10501 gctacacgtc gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac
    10561 cgccttatac gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc
    10621 acgtaaccat gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc
    10681 tagtagatta cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag
    10741 agtttgaaaa acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac
    10801 atgaacaaac agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag
    10861 tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac
    10921 gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc
    10981 tcacagatta tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa
    11041 cgctggaacg aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg
    11101 gaatcaattt gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg
    11161 atgcggataa agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag
    11221 ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc
    11281 gtctagcgat ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac
    11341 atgggatcgt tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag
    11401 aatcggaaga tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag
    11461 aaaaagacac gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg
    11521 attttgaaac agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg
    11581 aggacaacat ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa
    11641 atatagatgt aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg
    11701 gaaaaagttc cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga
    11761 gcctttctgc ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca
    11821 tcgacggatt gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt
    11881 cgacagtagg tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag
    11941 gacatccgat ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg
    12001 cagatcaagt aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg
    12061 taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc
    12121 gcgttcgagt cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca
    12181 aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc
    12241 gcctatttga ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg
    12301 tgatcggtga agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta
    12361 ctgttggcga actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag
    12421 actgtgatgg tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa
    12481 cgaagacact tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc
    12541 ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag
    12601 aattaccgga agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc
    12661 attatgaaaa tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga
    12721 agaatatcga gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc
    12781 tttatatgac agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc
    12841 tatctgttca aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag
    12901 cagtagattt cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga
    12961 ttttaaaaga agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa
    13021 ctttgagtcg cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta
    13081 cacaaattgg atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat
    13141 tgcatcaacg cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca
    13201 acaccctcgt tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg
    13261 acgtcggacc tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag
    13321 aagtagctaa tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc
    13381 ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag
    13441 aaaacaatct gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag
    13501 gtgtgtctgg ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc
    13561 aaaaattaaa taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa
    13621 gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc
    13681 cagcgactta tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg
    13741 caaaaatgcg gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg
    13801 aagcttgtcg cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg
    13861 ttccttgtga agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca
    13921 aaggaaaaag cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca
    13981 agcacattcc aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg
    14041 tgacgatggg gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg
    14101 ccagtgaatt gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga
    14161 caggacttca tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg
    14221 ctggtaacac agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata
    14281 tcattgattt aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac
    14341 cagaagaaat cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg
    14401 tataaagagg atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg
    14461 ttttattaat atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta
    14521 tagataaaaa cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact
    14581 ttcctttgat tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa
    14641 caagagatta tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc
    14701 cctatattat taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca
    14761 tacggcaaaa tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa
    14821 attgtcaatg tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg
    14881 cttcgtttgg caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt
    14941 ggtgatggat atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc
    15001 tgtccatata ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa
    15061 gtttgtgaag aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta
    15121 gtaaatggac atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca
    15181 aacagtgtgg agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag
    15241 actagagaag agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa
    15301 atgattgtac aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat
    15361 gtagatgaag atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct
    15421 acaccagcat cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat
    15481 caaaccatta aggcattttt ggaaaaaatc gaatatgcag gctttgccaa tttcgatatg
    15541 aagtatgatc ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt
    15601 agcagtttct ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga
    15661 gtgttcaaca aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga
    15721 ttatggatgg gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa
    15781 aaactagctg agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa
    15841 gaccgatcaa tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga
    15901 ttcaaaaagt attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta
    15961 ggcggaatgg gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa
    16021 gctacaaaag atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat
    16081 cgtacagctt atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat
    16141 attgagaaac aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac
    16201 tacttttttg aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa
    16261 gcagcaaatg aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa
    16321 ggaagtatga aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg
    16381 attcctgata ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa
    16441 tcggattatt tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta
    16501 aactgtgaaa aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa
    16561 gataatcatt acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt
    16621 gcattagctg aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt
    16681 tggggcaaat atcctaaaag
//
LOCUS       DACBZN010000058        16527 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.058, whole genome shotgun sequence.
ACCESSION   DACBZN010000058 DACBZN010000000
VERSION     DACBZN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.058"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(141..416)
                     /locus_tag="IVD64_001771"
     CDS             complement(141..416)
                     /locus_tag="IVD64_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP6149024.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /locus_tag="IVD64_001772"
     CDS             complement(659..1969)
                     /locus_tag="IVD64_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP6149025.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IVD64_001773"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IVD64_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP6149026.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="IVD64_001774"
     CDS             complement(3515..4195)
                     /locus_tag="IVD64_001774"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP6149027.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="IVD64_001775"
     CDS             complement(4244..4879)
                     /locus_tag="IVD64_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6149028.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="IVD64_001776"
     CDS             complement(4945..6348)
                     /locus_tag="IVD64_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP6149029.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="IVD64_001777"
     CDS             complement(6335..7366)
                     /locus_tag="IVD64_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149030.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="IVD64_001778"
     CDS             7397..7618
                     /locus_tag="IVD64_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP6149031.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="IVD64_001779"
     CDS             complement(7783..8520)
                     /locus_tag="IVD64_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP6149032.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="IVD64_001780"
     CDS             complement(8673..10013)
                     /locus_tag="IVD64_001780"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP6149033.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="IVD64_001781"
     CDS             complement(10250..11773)
                     /locus_tag="IVD64_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP6149034.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="IVD64_001782"
     CDS             complement(12151..12762)
                     /locus_tag="IVD64_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6149035.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="IVD64_001783"
     CDS             complement(13092..13808)
                     /locus_tag="IVD64_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6149036.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IVD64_001784"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IVD64_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP6149037.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="IVD64_001785"
     CDS             complement(14367..15164)
                     /locus_tag="IVD64_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP6149038.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="IVD64_001786"
     CDS             complement(15134..15505)
                     /locus_tag="IVD64_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6149039.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IVD64_001787"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IVD64_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP6149040.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3576 c         2798 g         5508 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacttgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttctttt
//
LOCUS       DACBZN010000059        16446 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.059, whole genome shotgun sequence.
ACCESSION   DACBZN010000059 DACBZN010000000
VERSION     DACBZN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.059"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(157..1524)
                     /gene="nhaC"
                     /locus_tag="IVD64_001788"
     CDS             complement(157..1524)
                     /gene="nhaC"
                     /locus_tag="IVD64_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6149041.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1633..3054)
                     /locus_tag="IVD64_001789"
     CDS             complement(1633..3054)
                     /locus_tag="IVD64_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6149042.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3297..5174)
                     /gene="tdc"
                     /locus_tag="IVD64_001790"
     CDS             complement(3297..5174)
                     /gene="tdc"
                     /locus_tag="IVD64_001790"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6149043.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5465..6721)
                     /locus_tag="IVD64_001791"
     CDS             complement(5465..6721)
                     /locus_tag="IVD64_001791"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP6149044.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7301..8602
                     /locus_tag="IVD64_001792"
     CDS             7301..8602
                     /locus_tag="IVD64_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6149045.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8671..9270)
                     /locus_tag="IVD64_001793"
     CDS             complement(8671..9270)
                     /locus_tag="IVD64_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6149046.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9524..10405
                     /locus_tag="IVD64_001794"
     CDS             9524..10405
                     /locus_tag="IVD64_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149047.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10418..12028
                     /locus_tag="IVD64_001795"
     CDS             10418..12028
                     /locus_tag="IVD64_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP6149048.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12105..12764)
                     /locus_tag="IVD64_001796"
     CDS             complement(12105..12764)
                     /locus_tag="IVD64_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6149049.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13047..13712)
                     /locus_tag="IVD64_001797"
     CDS             complement(13047..13712)
                     /locus_tag="IVD64_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149050.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13859..14464)
                     /gene="dhaL"
                     /locus_tag="IVD64_001798"
     CDS             complement(13859..14464)
                     /gene="dhaL"
                     /locus_tag="IVD64_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP6149051.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14492..15475)
                     /gene="dhaK"
                     /locus_tag="IVD64_001799"
     CDS             complement(14492..15475)
                     /gene="dhaK"
                     /locus_tag="IVD64_001799"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP6149052.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15477..15872)
                     /gene="dhaM"
                     /locus_tag="IVD64_001800"
     CDS             complement(15477..15872)
                     /gene="dhaM"
                     /locus_tag="IVD64_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP6149053.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            16026..16346
                     /locus_tag="IVD64_001801"
     CDS             16026..16346
                     /locus_tag="IVD64_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP6149054.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
BASE COUNT         4728 a         3408 c         3025 g         5285 t
ORIGIN      
        1 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
       61 agctgaaatt cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag
      121 ttaaacgctt ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt
      181 caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg
      241 caagtaatgc aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca
      301 cggtacgaga gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt
      361 tgataagcca acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag
      421 atactgttct ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa
      481 acgagaaggt cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata
      541 tttgatcagt aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat
      601 actcatgatc ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac
      661 ataaccattc atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa
      721 agaaagaacg agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt
      781 gattgccccc ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct
      841 cgctttcatg ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg
      901 tcctaatatc gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat
      961 accagcgata tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc
     1021 tgataaaggc gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat
     1081 tgcatcattg aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat
     1141 cgttgtaaat gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg
     1201 taaaaagaaa cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc
     1261 agataaaatc catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat
     1321 ccctggagtg attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa
     1381 tctgccataa aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg
     1441 gaatccgatg atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc
     1501 ttttaaagaa atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca
     1561 tgaaaaaact attcacttgg caggacctcc tactaaatca gcgacaaaac gtcggctgaa
     1621 agacttattt ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt
     1681 cttgaagtga ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa
     1741 taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa
     1801 gatcagcatg cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg
     1861 acgttcgaat tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta
     1921 cacgatacaa taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc
     1981 atccattaca ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac
     2041 atttctagat acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc
     2101 ttcacgagct acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa
     2161 aagcacacca ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag
     2221 gatttgacag tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg
     2281 tacgacattt gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc
     2341 gataaatacg ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga
     2401 actcatttcg atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat
     2461 atgttccaag tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac
     2521 tagtccgact ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata
     2581 tacccctagc catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa
     2641 gatcgtgatg atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc
     2701 aagttctaca ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac
     2761 catacctggg aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc
     2821 cagtgctgtt tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc
     2881 accggaaacc attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat
     2941 caatgtccaa ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat
     3001 tgtcagaccg ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga
     3061 cactctcctt gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac
     3121 gaaacaattc agcgaatatt tgaggggatt ctttgagttt attgcttgaa gggtttcttc
     3181 aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc
     3241 aaacatgcgt cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat
     3301 tttgcttcgc ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct
     3361 gctttgattt tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt
     3421 acgctggcac gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct
     3481 agttggttca caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt
     3541 gaagttaaga actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg
     3601 tcatggttca atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag
     3661 tctaccatat tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact
     3721 ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc
     3781 attaatttac catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg
     3841 gctgctgttg cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata
     3901 tctgcgcctt tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata
     3961 tctttgatga cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc
     4021 gttacagatt ctgcttcttc gattgcttta tatgcactgt gtacttcttc taagatataa
     4081 tctttgtttt ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga
     4141 atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct
     4201 acgtgtaagt agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta
     4261 tcgataccgt cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt
     4321 ggtgtttttt cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga
     4381 tagttgtggt caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca
     4441 gcttttagcc atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa
     4501 tgttttccgc tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct
     4561 aacagattca tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca
     4621 actagttcag gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac
     4681 caaagtcctt ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta
     4741 tagctcatta attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga
     4801 gaagattcat aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa
     4861 gatggcatca atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga
     4921 acggaatggg tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca
     4981 gtgtgttcaa aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc
     5041 atgtagtttt gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag
     5101 atttggccgt tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac
     5161 aatgattcac tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca
     5221 ttacacctgt tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa
     5281 tatgtatcaa ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt
     5341 tacaaatgta aactccctcg caaagattgt attaacgaca accagcgttc tattttagga
     5401 tgggtttcaa aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta
     5461 aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa
     5521 acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac
     5581 cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa
     5641 tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg
     5701 gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga
     5761 acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg
     5821 taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt
     5881 tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta
     5941 catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg
     6001 gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca
     6061 tcaatgggat cgttaaaccg aacgcttctg cttctggtcc ttctaatttt cggattaggt
     6121 ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt
     6181 atgttttatg cagatgcaaa aagtcgatag attgcaggat ttggtaagta aactctgtaa
     6241 atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt
     6301 tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt
     6361 agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa
     6421 gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc
     6481 cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg
     6541 ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac
     6601 cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc
     6661 tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca
     6721 ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca tgtgtaatta
     6781 attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga
     6841 cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat
     6901 ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc
     6961 atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt
     7021 ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg
     7081 tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta
     7141 attccgaaca ttttattttc aattattgtt aaaagaaata actttttctg tattttttct
     7201 gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa
     7261 tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat
     7321 tattttagga gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt
     7381 catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc
     7441 aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag
     7501 ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga
     7561 aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga
     7621 tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt
     7681 ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg
     7741 ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg atttgatcca
     7801 aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc
     7861 attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc
     7921 tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa
     7981 agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc
     8041 tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt
     8101 aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa
     8161 agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac
     8221 atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt
     8281 gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg
     8341 tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa
     8401 gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt
     8461 aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga
     8521 tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa
     8581 agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc cgaactgaca
     8641 ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga ttcccttgat
     8701 caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct ccggcttaga
     8761 aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat atttcaaatc
     8821 cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga aactttcttg
     8881 cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat agtatgtcat
     8941 caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt tttcactcat
     9001 ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg cttcttgccc
     9061 taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc tgccaaaata
     9121 tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca ttgtcactcc
     9181 agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt
     9241 ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt
     9301 ataatatata tagttcaaat ttattctgag gtttattata actatctatt gagaaagtat
     9361 caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg
     9421 tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta
     9481 atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa
     9541 agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac
     9601 cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac
     9661 gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca
     9721 agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat
     9781 ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg
     9841 aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa
     9901 aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc
     9961 tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga
    10021 aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc
    10081 ttctcatatt ctcagtgaag tcgagcgact agcggataaa gttgtgatca tacgcgaagg
    10141 aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac
    10201 tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt
    10261 gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc
    10321 tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt
    10381 attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg
    10441 gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt
    10501 cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca
    10561 aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc
    10621 aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt
    10681 gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag
    10741 aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc
    10801 aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat
    10861 cagttgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg cttggctttt
    10921 tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca
    10981 actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta
    11041 ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg
    11101 ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt
    11161 tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa
    11221 caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt
    11281 gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg
    11341 tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa
    11401 cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag
    11461 cacgatcatg gtcgtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg
    11521 tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag
    11581 gaaccaactt tattggacca atatgttact agctattcta tttgctatat tggcaatcct
    11641 gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat
    11701 ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat
    11761 cggaattagt ggctttctat tcggatggct tcctcgaatc ggtaaacttg cttatatcta
    11821 cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt
    11881 ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc
    11941 cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta
    12001 taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc
    12061 caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac
    12121 tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct
    12181 aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct
    12241 ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga
    12301 tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact
    12361 tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt
    12421 aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg
    12481 ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc
    12541 tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg
    12601 ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt
    12661 tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga
    12721 ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc tcctccttct
    12781 aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata attttctttt
    12841 atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt tctttttgtc
    12901 ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca attttttaaa
    12961 tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag taaggtttgt
    13021 ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag cttttgatat
    13081 agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc gatttcttgc
    13141 ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt tttcaactct
    13201 tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc aagttcatgt
    13261 tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca ctctttcagt
    13321 atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct tttataatgt
    13381 tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag acaggcattc
    13441 tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa attccagcca
    13501 ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa aagtgcagct
    13561 gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt cttgatttgt
    13621 tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat tggtcgatca
    13681 aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt tatcttttgt
    13741 attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg ttctgcatca
    13801 agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt tattgaactt
    13861 aaggttgatt gatcacatct aaaagcgttg aaaataaaat ggcactagaa gcagctcctg
    13921 ggtcgatatg tccgattgca cgctctccta gataggaagc tcgtcctttt ttagctacca
    13981 agactttggt gtgttctttt gcctggtcaa tgatttcttt tgttaaattc cctgccttca
    14041 atgcctctgt gactggcagc caaacatcta acatcgtttt atcgtcagct tctgcctttc
    14101 ctcggctttg aatacccgct gttccctgct cgatcacttc gcctaatttt tcttgagaat
    14161 caatagaatc gacctctttt gtcgctttca tcatgttcat gaatgcagat ccgtataacg
    14221 gaccagaagc cccaccgact ttcgatatca tcgccataga gaatactttg aatgtatcgc
    14281 tgattgtttc aggatgatcg gcttgaaaag cttcttcata agctgaaacc cctcttccca
    14341 tgttatttcc atggtctcca tcgccaatcg gggtatctag atcactgaga tattgtttgt
    14401 tctgattgat tgcctctgca aattgcgtga gccattcttg gatttcttga acggttagtt
    14461 tcatattatc acccttttat cttgagattt tttaccatga aatcgtctgt acattgcttt
    14521 ctaaagaatc tttccattgt gtctccgtca agtcgatcag tgttagagaa agaccttgca
    14581 tatctagtga ggtcataaaa ttaccgactt ttcggaaaac tacattgacg cctctttctt
    14641 ccaacagagc taaaacatca ttcatgaaca cgaactgttc catcaaaggc gtcccgccca
    14701 ttccgttgac tagtacacct gcttcttttg gtaaattcga gtaatcatct aaaatcttag
    14761 taacaagttc ctttgccagt actttagaag gctgcatctt ttctcgacga tagcctggct
    14821 caccgtggat accaacacca tactcgatct catcttctcc tagttcgaat cctggtttcc
    14881 ctacttctgg tacagttgct gctcgaagcg ctagaccgat cgtcttagtt gcctgtacga
    14941 ccttttctcc cagttctttc aactcagcta gggaagcacc gtttcttgct gcatcgccca
    15001 cgattttgtg tacaaggaca gtacctgcaa ctccgcgttt tccagcagta tacgtactat
    15061 tttcaacggc gatatcatca tctacgatca cactttctac ttcaatatcg tccatagcag
    15121 ctaattcctt tgccatatcg aagttcaatg catcccctgt ataattctta acgatcagca
    15181 aaactccttt tccactatcg gcttccttaa tagccgtctg aatctgatct ggagttggag
    15241 aagtaaacac atcaccaagc accgctgcac ttaacatccc ttcccctaca aacccggcat
    15301 gggatggttc atgtccgctt cctccaccag aaaccaaacc aacttgttcc gctttctgat
    15361 tcttggctac aacacgggaa gattctacac gatgaacaag ttctggatag ctttttacta
    15421 atccttccag catttcttcg acaatattgc ttggatcatt catgattttt ttcaatttat
    15481 tcccatcctt tctttccggc attttgtgct tctgttaaga tttgctgagt atcatctgtt
    15541 ataccggcag tgatggctgc tgcaaaagct ccttccacta aaggcgcatc caccagatga
    15601 taacgttgct gctgctcttc ttccaacatg tcttttgcca gttccgagtt caataccgcg
    15661 ctaccgatat ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt gactgcttct
    15721 acgattttca tcgggtctga tccgatctcg ccatttattc cgccgagaga aaaaatttgc
    15781 acctcttcgg atttttgcat ctgctcaatc atttctttaa taccatctgt tactttttga
    15841 ctatgagaaa taagcaaaat actttttttc acaagaatct tcctttcttt tacatagttg
    15901 ctaacgattg gtttcgcttt cattaatgaa caattcaagt atataataaa tttctaattt
    15961 tgggaaaata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa gagaggagtg
    16021 atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta gcttataaag
    16081 ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg gaggcggggt
    16141 tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg ggtgcaagag
    16201 aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat gcgatcattg
    16261 gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg gtcacatgta
    16321 gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac taaagagaga
    16381 tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag gtactctacc
    16441 ttccca
//
LOCUS       DACBZN010000060        16399 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.060, whole genome shotgun sequence.
ACCESSION   DACBZN010000060 DACBZN010000000
VERSION     DACBZN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.060"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(296..1024)
                     /locus_tag="IVD64_001802"
     CDS             complement(296..1024)
                     /locus_tag="IVD64_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149055.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IVD64_001803"
     CDS             complement(1002..2576)
                     /locus_tag="IVD64_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6149056.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIAPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IVD64_001804"
     CDS             2805..3743
                     /locus_tag="IVD64_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6149057.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IVD64_001805"
     CDS             3755..4666
                     /locus_tag="IVD64_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6149058.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IVD64_001806"
     CDS             4690..6162
                     /locus_tag="IVD64_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6149059.1"
                     /translation="MKKNMQKYLIGGVSVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6214..6825
                     /locus_tag="IVD64_001807"
     CDS             6214..6825
                     /locus_tag="IVD64_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6149060.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6855..8768
                     /locus_tag="IVD64_001808"
     CDS             6855..8768
                     /locus_tag="IVD64_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP6149061.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8896..11877
                     /locus_tag="IVD64_001809"
     CDS             8896..11877
                     /locus_tag="IVD64_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP6149062.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11874..13535
                     /locus_tag="IVD64_001810"
     CDS             11874..13535
                     /locus_tag="IVD64_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP6149063.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13575..14507
                     /locus_tag="IVD64_001811"
     CDS             13575..14507
                     /locus_tag="IVD64_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP6149064.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14867..16360)
                     /gene="guaA"
                     /locus_tag="IVD64_001812"
     CDS             complement(14867..16360)
                     /gene="guaA"
                     /locus_tag="IVD64_001812"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6149065.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         5239 a         2689 c         3302 g         5169 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gcgcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt acaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgtttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgacga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttta ctaaatgaaa
     6181 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     6241 tttaattggg tttttcaatt gttattacta aactttactt attttatact aatttgttta
     6301 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     6361 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     6421 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     6481 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     6541 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     6601 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     6661 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     6721 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     6781 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     6841 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     6901 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     6961 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atctgcttgt
     7021 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     7081 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     7141 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     7201 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     7261 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     7321 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     7381 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     7441 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     7501 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     7561 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     7621 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     7681 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     7741 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     7801 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     7861 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     7921 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     7981 tgttaactac ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg
     8041 cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct
     8101 acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg
     8161 aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt
     8221 ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca
     8281 ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt
     8341 ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa
     8401 ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc
     8461 ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat
     8521 tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat
     8581 tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa
     8641 atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc
     8701 aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct
     8761 acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg
     8821 ttcgttttct attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt
     8881 aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat
     8941 acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag
     9001 ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat
     9061 tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagttat
     9121 gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc
     9181 aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt
     9241 gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa
     9301 ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg
     9361 agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt
     9421 caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga
     9481 atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt
     9541 tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt
     9601 caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat
     9661 ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa
     9721 gattgccttt tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat
     9781 ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta
     9841 ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa
     9901 ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta
     9961 aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg
    10021 gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat
    10081 ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg
    10141 attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg
    10201 tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca
    10261 cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac
    10321 caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat
    10381 gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc
    10441 cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat
    10501 gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact
    10561 ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat
    10621 ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac
    10681 cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc
    10741 ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat
    10801 atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa
    10861 agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt
    10921 tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa
    10981 gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa
    11041 ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct
    11101 gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca
    11161 atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga
    11221 tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc
    11281 ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac
    11341 atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat
    11401 ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg
    11461 aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg
    11521 atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa
    11581 gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc
    11641 atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc
    11701 gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat
    11761 aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt
    11821 atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag
    11881 aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa
    11941 accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc
    12001 gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac
    12061 agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc
    12121 atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat
    12181 cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg
    12241 taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc
    12301 gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt
    12361 tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat
    12421 tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc
    12481 ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg
    12541 atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga
    12601 ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag
    12661 gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag
    12721 tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat
    12781 atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat
    12841 taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc
    12901 aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg
    12961 attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc
    13021 tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag
    13081 cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag cgctttgatc
    13141 ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt
    13201 atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag
    13261 aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag
    13321 cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt
    13381 ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc
    13441 caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag
    13501 taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga
    13561 aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa
    13621 tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg
    13681 aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag
    13741 catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg
    13801 gactaccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga
    13861 ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc
    13921 ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg
    13981 aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag
    14041 gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt
    14101 cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata
    14161 aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag
    14221 ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat
    14281 atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat
    14341 tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg
    14401 ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag
    14461 gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa
    14521 aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc
    14581 attttaaaaa aagggactgt gacagaagtg aaaatagaca aaaaatatcc gaacaagaga
    14641 ctaggatagc agaaaaacta tcgttttgca acgaggacca actggaacgt aggagcaatt
    14701 ctcttattgt tcggatattt cgcttgttca aggacttatg tctgtagtgc ccttttttgg
    14761 taggaaaaat ggtagggaac catatcattt ttaatgaaaa tcaagcattt caaaagttga
    14821 aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat tcccactcca
    14881 ctgttgcagg tggtttgctg gtaatatcat acacgatacg gttaacgtgt gcgacttcat
    14941 ttacgatacg gacactgatt ttttgcaaga cttcccaagg gatacgggca aagtcagcgg
    15001 tcatgccgtc aatagaagtg acagcacgga tacctacagt gtagtcatac gtacggccat
    15061 ctcccataac cccaacagag cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc
    15121 gatctaaacc ggcattggcg atttcttcgc gcaagatagc gtctgattca cggacgattt
    15181 ctaatttttc ttctgtcaat tcaccaagga cacgaatacc taagcctggt ccagggaatg
    15241 gttggcgcca aacgatgctg tcaggcatgc ctaattgggt acctaattca cgtacttcgt
    15301 ctttgaacaa tgtgttcaat ggctcgatca gcttgaattg catgtcttca ggtaatccac
    15361 ctacattgtg atgtgatttg atggtttggg cagtttcagt tcctgattcg atcacatcgg
    15421 tataaagggt tccttgtgca aggaaagaaa cgccttcttc accggcaagt ttggttgctt
    15481 catcatcgaa gacatataca aattcatttc caatgatttt acgtttttct tctggatcag
    15541 acacgccagc aagttttgat aagaaacgtt ctttggcatc gacttttata atgttcaagc
    15601 caaattttcc gccaagactt tccataactt gttctgcttc acctttacgc aacaatacgt
    15661 gatcaacgaa gatacatgtt aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa
    15721 cagaggaatc tactccgcct gaaaggccta ataaaacttt tttgtcaccg acttgttcac
    15781 gaatttttgc gatctccatg tcgatgaagt tatccattgt ccagtcacca gtacattggc
    15841 agacatctag tgtaaaatgg cgcaacaagt catttccata ttcggaatgg cgaacttctg
    15901 catggaattg gattccgtac attttgcgtt ctgcattttg gatcgatgcg atggggcaat
    15961 ctttgctggt agcaacgaca tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac
    16021 tcatccaaac ggtttgtttt tcaggagtgt tttggaaaag ggccgcttct gcagaagtga
    16081 tttccaattc tgcttttccg tattcacggt ttttggctgg tttaactttt ccgcctaatt
    16141 tataagtaat caactgcata ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa
    16201 tcttttcatc aataccaaag gccttctcat cataaacact gtttggtccg ccagaaagga
    16261 tgattccttt agggttcatt gcctttactt cttctgcggt aatgcggtgg ctcatcaact
    16321 ctgagaaaac accgaactca cgaatacggc gagtgatcag ttggttatac tgactaccgt
    16381 aatcaagtac gataatttt
//
LOCUS       DACBZN010000061        16271 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.061, whole genome shotgun sequence.
ACCESSION   DACBZN010000061 DACBZN010000000
VERSION     DACBZN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.061"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(56..712)
                     /locus_tag="IVD64_001813"
     CDS             complement(56..712)
                     /locus_tag="IVD64_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6149066.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            905..1183
                     /locus_tag="IVD64_001814"
     CDS             905..1183
                     /locus_tag="IVD64_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149067.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1202..2410
                     /locus_tag="IVD64_001815"
     CDS             1202..2410
                     /locus_tag="IVD64_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6149068.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2558..2923)
                     /locus_tag="IVD64_001816"
     CDS             complement(2558..2923)
                     /locus_tag="IVD64_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP6149069.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3070..3843)
                     /locus_tag="IVD64_001817"
     CDS             complement(3070..3843)
                     /locus_tag="IVD64_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6149070.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4017..4877)
                     /locus_tag="IVD64_001818"
     CDS             complement(4017..4877)
                     /locus_tag="IVD64_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP6149071.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4877..6295)
                     /locus_tag="IVD64_001819"
     CDS             complement(4877..6295)
                     /locus_tag="IVD64_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6149072.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6352..6801)
                     /locus_tag="IVD64_001820"
     CDS             complement(6352..6801)
                     /locus_tag="IVD64_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6149073.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7246..7509)
                     /locus_tag="IVD64_001821"
     CDS             complement(7246..7509)
                     /locus_tag="IVD64_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP6149074.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7608..8903)
                     /locus_tag="IVD64_001822"
     CDS             complement(7608..8903)
                     /locus_tag="IVD64_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP6149075.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8900..10171)
                     /locus_tag="IVD64_001823"
     CDS             complement(8900..10171)
                     /locus_tag="IVD64_001823"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6149076.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10336..11205)
                     /locus_tag="IVD64_001824"
     CDS             complement(10336..11205)
                     /locus_tag="IVD64_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP6149077.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11497..13107)
                     /locus_tag="IVD64_001825"
     CDS             complement(11497..13107)
                     /locus_tag="IVD64_001825"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP6149078.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="IVD64_001826"
     CDS             complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="IVD64_001826"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP6149079.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15668..16060)
                     /locus_tag="IVD64_001827"
     CDS             complement(15668..16060)
                     /locus_tag="IVD64_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6149080.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4639 a         3450 c         2766 g         5416 t
ORIGIN      
        1 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt gttcatcaaa
       61 agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg tctttaacgg
      121 tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta gcagttgaga
      181 tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc atcccttctt
      241 ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca gctcccagct
      301 ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga tagcggtaat
      361 actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa aagcgcacac
      421 gattgattcg ataaaactct gcatattctg attcaactcg aatattatac ggcgaatcga
      481 tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg atatagcgaa
      541 ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc tctttcaaga
      601 tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat cctttgcgta
      661 taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc aatgatgctc
      721 ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt taaagtccaa
      781 aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac ttatgcgatt
      841 tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa tagagggaaa
      901 aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat atcaaacaag
      961 atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact cgatcgaatt
     1021 gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa atctgtttat
     1081 gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca attatcacga
     1141 tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat gtataacata
     1201 catggaaaag aaattattta ataaatattt tattggaatc acattgatca attttattgt
     1261 ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag acaccttaca
     1321 cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa cattgctggc
     1381 acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct taagaggcgg
     1441 cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta tcggggcatt
     1501 gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag ctacaaatgc
     1561 tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt attatggatt
     1621 aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc tgaatatgac
     1681 tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta cgattgcctg
     1741 ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat ctcttcgctc
     1801 atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa ttgccttttt
     1861 gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa aagtaatcca
     1921 cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca catttacacg
     1981 tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta
     2041 tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg tgacattgct
     2101 tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg gacaggctgt
     2161 ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct actttattgg
     2221 tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat ctcttacttc
     2281 atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag cactctatct
     2341 tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa
     2401 tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa taatctgagc
     2461 ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt tatttttaaa
     2521 gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc cgttcatctg
     2581 gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca ccgatcaata
     2641 gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa tatagataga
     2701 gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt
     2761 tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt accccaacaa
     2821 tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac agaatcgccc
     2881 agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc actcctttct
     2941 cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat accatcggct
     3001 gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt agaacattca
     3061 cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg catactgttc
     3121 ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa tggcttgatg
     3181 catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt ctgattgttt
     3241 tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc cttggctgaa
     3301 atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg catcgttata
     3361 ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca atacagatac
     3421 ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg taatgatctg
     3481 caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac tggaatccaa
     3541 gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct tacgtttttc
     3601 tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt ttcgattgtc
     3661 aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca gaaaaacaag
     3721 gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg tgactcttgc
     3781 aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca cttcattcaa
     3841 cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt gacagaagtg
     3901 attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac atcgtgtgtg
     3961 gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt ttatttttag
     4021 tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac cacttcttgt
     4081 acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg attttcactc
     4141 aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa attcactttg
     4201 gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga accgccatgt
     4261 aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat atttaatttt
     4321 ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc aagtgtatcg
     4381 attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat gattgtttca
     4441 gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc cactgagaca
     4501 tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc gtcgaatgat
     4561 ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg taagacatct
     4621 tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt ataagcatag
     4681 gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg atgaatttct
     4741 aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat catatcatag
     4801 ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt caataactct
     4861 ttcattgtat ataacattac gcttccacct catttgttag attttcattt ttcgtccact
     4921 tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag gctgtagtga
     4981 ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca tttggaatta
     5041 gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc atggaaatcc
     5101 ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg tcagataccg
     5161 caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat gattgacctg
     5221 aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa gcaataccta
     5281 ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact ccagctgaca
     5341 tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata tcagctgcca
     5401 tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag ctattcagcc
     5461 aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat accatcaaaa
     5521 gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata gcagaaaatt
     5581 ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct agcacgccac
     5641 caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg gcaacaattc
     5701 ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct aacgcaggaa
     5761 ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct aacacattat
     5821 agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt gcgatgatga
     5881 ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa tgattataga
     5941 ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg aatacgccaa
     6001 tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg cttgtagaga
     6061 cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct actgcaagaa
     6121 tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt tttacacctt
     6181 gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat ttttctgccg
     6241 acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt ttcatgatta
     6301 ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt ttcattcttg
     6361 actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct ttctaaaatc
     6421 tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat ctgctgattt
     6481 ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc cgctatagtc
     6541 cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt tgctttttgc
     6601 atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc gtttgaatag
     6661 tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca catcccctat
     6721 tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca gttcttctgc
     6781 gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta acgttttctt
     6841 ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa tgaacagagg
     6901 aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc tcacaaacaa
     6961 tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc ttctaatttt
     7021 aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat aaggtgtttt
     7081 tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca ttatgatcct
     7141 acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa aaacagacta
     7201 tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt ccgcagcgtt
     7261 tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg cttgtgtgaa
     7321 tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac ggatgactgg
     7381 gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg aacctgacaa
     7441 gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg gatggatgtt
     7501 ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag agttgatttg
     7561 tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta tcttttcaga
     7621 tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa aaattgctga
     7681 ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa tgaatctcca
     7741 gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat caataattct
     7801 tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg ttcagataat
     7861 tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata gatcacgtca
     7921 tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact gccaccttct
     7981 tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc tgggtcaatc
     8041 ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg agcaagtcta
     8101 gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg cattgcgcgt
     8161 tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa tgtagaagat
     8221 accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg ttcatcaatc
     8281 agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc gatttctttt
     8341 aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg accaaaaccg
     8401 attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt ttccagttga
     8461 agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc ttttgcttct
     8521 tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg ttccgattct
     8581 ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa tcgtctgatt
     8641 tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg ccgtaaaaaa
     8701 ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa tccttgtttc
     8761 tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt actgtaataa
     8821 ggaattttaa attctttggc ataactataa acttctttta acgttttgtt ctctagttct
     8881 gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca attgatgccc
     8941 ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa tctacattgt
     9001 aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca cctgcccgaa
     9061 gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg atgatcatat
     9121 ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg ttcacacgtt
     9181 tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt aacgcagtaa
     9241 gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg tttgcgggtt
     9301 ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata tggacaccaa
     9361 tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt ttcactttga
     9421 taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg cggtcaggaa
     9481 tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc acatctgtac
     9541 ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct atgatttccg
     9601 gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca gctaacatca
     9661 cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca cgcaatcctt
     9721 tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca cctagagctt
     9781 caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca cctggcaggc
     9841 ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa gaagcacgca
     9901 aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga tcgatttcta
     9961 gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca attaatgaat
    10021 gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct aagattgcag
    10081 ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca ccagctaaag
    10141 gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat taatcttttt
    10201 tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg gtctgccgtt
    10261 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
    10321 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
    10381 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
    10441 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
    10501 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
    10561 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
    10621 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
    10681 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
    10741 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
    10801 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
    10861 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
    10921 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
    10981 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
    11041 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
    11101 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
    11161 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
    11221 aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta ttttaccaaa
    11281 catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa gttttttgca
    11341 aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc tatctttttt
    11401 cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc ttatcccctt
    11461 ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt gatctgctaa
    11521 tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag aaatcaattc
    11581 tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga tttctactaa
    11641 acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca attgacgata
    11701 tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg ctccatcata
    11761 tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa ggcgtaaagt
    11821 tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat tgtttgctgt
    11881 atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac gtgcaaattc
    11941 gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat tttcgcgagc
    12001 ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac caggaaccaa
    12061 gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt cttgtgcttt
    12121 gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca atgcttcaac
    12181 tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa tccgtacttt
    12241 tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt ctgcttctgg
    12301 tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt gtaaaactaa
    12361 tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg gttcgacatc
    12421 acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg tacgtaccac
    12481 caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct gcgttggttt
    12541 agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga tatacataac
    12601 attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg gcaaagattc
    12661 tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag tcattttagc
    12721 tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt gaacagttgc
    12781 tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt ttcctgtcgt
    12841 cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc ccagatccaa
    12901 atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg ggctcatcgt
    12961 ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta agccgcggtt
    13021 tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg aaacgacacc
    13081 gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat ttatgtgcag
    13141 gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg gagccgaaat
    13201 ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa gctgactaag
    13261 ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt tcgatatcct
    13321 ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc acataataag
    13381 cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt actgaaaaat
    13441 tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa tatcccacct
    13501 aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc gtagaaacaa
    13561 tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca cagttctcgg
    13621 atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc actaattttt
    13681 tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa ggtaacgaat
    13741 cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt ctaaataaac
    13801 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca tgagaagctg
    13861 tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc tctggataaa
    13921 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagcc
    13981 attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa cctcttggta
    14041 aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa aacaagaaaa
    14101 gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca caacctctcc
    14161 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    14221 gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt cacagcctct
    14281 aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc cactcggaaa
    14341 cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt gagaagatat
    14401 gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata gcattatgga
    14461 tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc attacataaa
    14521 tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct agtcctgctt
    14581 gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac cgcaattcca
    14641 aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga ttacggccac
    14701 tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa gaattgactg
    14761 ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct aagaaatgat
    14821 aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt ccttcatcat
    14881 tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac ggtttcggca
    14941 tgaaagtcgc gtgaagtcca tgcttgcgcg caatcgtttt gacaacgagc ttgaaggttt
    15001 ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca tgctgtcctg
    15061 gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat tccaacacga
    15121 tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa ccacctttat
    15181 cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag aattctggtt
    15241 caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat gctcgtttca
    15301 aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata tcacaaatta
    15361 aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg gagagatctg
    15421 gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa gaaccgtcaa
    15481 acatcatttt attttctaat actttgtcga tctggctcac tggaacttct acatttttga
    15541 tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct tcctcaatga
    15601 ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc ttcctttttt
    15661 aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga cctgttttgc
    15721 ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca tgatctgtca
    15781 acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat acacgtttga
    15841 tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac atatcaatat
    15901 cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact aaatcttgtt
    15961 cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact gtaccaattg
    16021 gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc ctttcgtttt
    16081 ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat gttaggtttt
    16141 ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca aaaaaggtat
    16201 ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta tcctttatta
    16261 gtgtttttat t
//
LOCUS       DACBZN010000062        15548 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.062, whole genome shotgun sequence.
ACCESSION   DACBZN010000062 DACBZN010000000
VERSION     DACBZN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.062"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(28..1791)
                     /locus_tag="IVD64_001828"
     CDS             complement(28..1791)
                     /locus_tag="IVD64_001828"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP6149081.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(2012..3472)
                     /locus_tag="IVD64_001829"
     CDS             complement(2012..3472)
                     /locus_tag="IVD64_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149082.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(3680..4606)
                     /gene="glsA"
                     /locus_tag="IVD64_001830"
     CDS             complement(3680..4606)
                     /gene="glsA"
                     /locus_tag="IVD64_001830"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP6149083.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(4629..5840)
                     /locus_tag="IVD64_001831"
                     /pseudo
     CDS             complement(4629..5840)
                     /locus_tag="IVD64_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(5875..7488)
                     /locus_tag="IVD64_001832"
     CDS             complement(5875..7488)
                     /locus_tag="IVD64_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP6149084.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(7600..7809)
                     /locus_tag="IVD64_001833"
     CDS             complement(7600..7809)
                     /locus_tag="IVD64_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP6149085.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            7900..8088
                     /locus_tag="IVD64_001834"
     CDS             7900..8088
                     /locus_tag="IVD64_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149086.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(8798..10012)
                     /locus_tag="IVD64_001835"
     CDS             complement(8798..10012)
                     /locus_tag="IVD64_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6149087.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(10140..10610)
                     /locus_tag="IVD64_001836"
     CDS             complement(10140..10610)
                     /locus_tag="IVD64_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6149088.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(10607..10849)
                     /locus_tag="IVD64_001837"
     CDS             complement(10607..10849)
                     /locus_tag="IVD64_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP6149089.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(11395..11925)
                     /locus_tag="IVD64_001838"
     CDS             complement(11395..11925)
                     /locus_tag="IVD64_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6149090.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(12107..12763)
                     /locus_tag="IVD64_001839"
     CDS             complement(12107..12763)
                     /locus_tag="IVD64_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP6149091.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(12890..13363)
                     /gene="dfrF"
                     /locus_tag="IVD64_001840"
     CDS             complement(12890..13363)
                     /gene="dfrF"
                     /locus_tag="IVD64_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP6149092.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(13782..14000)
                     /locus_tag="IVD64_001841"
                     /pseudo
     CDS             complement(13782..14000)
                     /locus_tag="IVD64_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14031..15245)
                     /locus_tag="IVD64_001842"
     CDS             complement(14031..15245)
                     /locus_tag="IVD64_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6149093.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4571 a         3262 c         2413 g         5302 t
ORIGIN      
        1 aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca gactatcttc
       61 aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag gaacgctatc
      121 cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac cataataacg
      181 actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga tgactaattc
      241 gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg ttcctggatt
      301 gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt ttcctaattc
      361 aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg tttgataggc
      421 tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg taggaattcg
      481 gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga tcatatcctc
      541 ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag gaatgtcaaa
      601 tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta aaataatcac
      661 tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt gcaagaaagt
      721 acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag cgaaagtgat
      781 agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt cttggaatgt
      841 tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag ctgtacttgt
      901 ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc cagaagttac
      961 ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt tcattttatt
     1021 atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta caatcggatg
     1081 atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt ttccattatc
     1141 cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt taccgttggt
     1201 ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa tagacatctc
     1261 attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt tcaatccttc
     1321 aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat ttcctgattc
     1381 tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg tagatgtgtg
     1441 ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc tcacttcacg
     1501 gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac cataagactg
     1561 atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat gaaacgcaat
     1621 cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc tgtccttttg
     1681 caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag ataatttttt
     1741 gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca taatacattc
     1801 tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat ctattctact
     1861 acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa gcttgttata
     1921 taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac aattaaaaag
     1981 ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt aataggtgaa
     2041 gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt ggatagatct
     2101 ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa accactcttt
     2161 ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa ataggattta
     2221 tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa aattgtttga
     2281 ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt tcactgttga
     2341 tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt agttggattt
     2401 gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga agttgttctt
     2461 tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc cacaacgtac
     2521 ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg atttcagcga
     2581 tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca aaatttttat
     2641 aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa tttctactgt
     2701 tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt cctttttgct
     2761 cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt ttcttatctt
     2821 cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt tctcgtatga
     2881 taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct acataatcaa
     2941 tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct tcccaattga
     3001 cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata atgatttctt
     3061 tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg attgtatata
     3121 ctttactcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat ataccattaa
     3181 aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag
     3241 aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt
     3301 ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag
     3361 tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt
     3421 cacacagata cacttttcta aatatatttc gctcgatgta attttccagc attcctgata
     3481 cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt
     3541 cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc
     3601 aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc
     3661 cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt
     3721 tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac
     3781 ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag
     3841 gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa
     3901 ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa
     3961 ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg
     4021 agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac
     4081 ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga
     4141 tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc
     4201 gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg
     4261 cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat
     4321 tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt
     4381 ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat
     4441 cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt
     4501 gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat
     4561 agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct
     4621 ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga
     4681 ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca
     4741 gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga
     4801 cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca
     4861 agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc
     4921 aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg
     4981 cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaatga
     5041 gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct
     5101 gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct
     5161 aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt
     5221 aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact
     5281 gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca
     5341 aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg actggcagat
     5401 gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc
     5461 gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg
     5521 acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa
     5581 ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc
     5641 cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata
     5701 cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt
     5761 atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg
     5821 acccaagtat agacaccgcc ggtttccctt agttaaaaat attactcttg ttgattactc
     5881 aactcttcca ataaatcggt aatatatctc aatttcctgt attctttcgt tatcctcatt
     5941 cgttgttgct tcatgtatga ggattttttc tatcatggca tttaataatg gtgctgtcag
     6001 ttcctttggt acggaatatt ccttgattaa ctcaatccac ttttcagcac ctatcatatc
     6061 ctgttccatt ttacttagtt cttctcttag gcttgttatc tgctgttcca gttctatctg
     6121 ttctttttgg tacttgctgg acaacatcac aaaatttcgc tctgttatct tctcacaggc
     6181 tctatcttca tacattttcg caaataaacg gtcaatctca ttttgtctgt tctcggcttt
     6241 cttcaatgta cttgcctttt tcttcttttc ccgtattctc tctgcattgc cagctttctg
     6301 tatcttgttc aatacctttt cttcgtcctg ctgtactgcc ttagcccaat actgcaagcg
     6361 ttccaatacg gcttgataca gcacatcata acgaatgtag tgcatagaac aattaccttt
     6421 gccaaactgc ccgtagtaac tgcaagcata ataactgtaa ggcgttttat ttgtcttatt
     6481 cgttccaaat ctcatagacc aaccacaatc cgcacacttg acaagccctg caaaaatcgg
     6541 cgttgccttt tcctttgtct gtctgcgtct tgattttatc tgctcctgca cacgatagaa
     6601 cacttccttg tcaataatcg gctcgtgagt gttttctact cgaaaccatt cactttcggg
     6661 cttacgcact ttctttttgc tcttgaacga taccgtagac tgcatattgt gaacactgtt
     6721 tccgatataa acctcacttt tcaatatcgc cttgacatga gcaatcgtcc actcataacg
     6781 cttactttcg ggttttccct caaagatatg tgcaaaagta ccgtatcttg taaaatttag
     6841 ccatgacgct gtcggaactt tttctgctct taactgcttt gtgatttttg cacttccgta
     6901 accttgatag gctagggaaa agattttttc aataatccat tttgtttcat catcaaccaa
     6961 cagttttcct ttgatgtcag ggtgtttctt atatcctaac ggagcataag caccgtaatg
     7021 agccccctcc gcaaactttg tcttaaatgc tgatttgact ttacggcttg tatctcttgc
     7081 cacccattca ttgatgatgt tcttaaatgg tgcaatctca ctttcgcctt tttcgctgtc
     7141 tacaccgtca tcaatcgcta tgtagcggac attatgacta gggaaataca attctgtata
     7201 ttgtcctgtc ataatataat ttctgccaag acgggaaagg tctttcgtta caacacagtt
     7261 gatttttcct gcctctatat cctcaatcat tctttgaaaa ctcggtctgt caaaatttgt
     7321 tcccgaccag ccgtcatcaa tatattcatc aatgacattc aaatgatgtt cttttgcata
     7381 taaacgcaac atacttctct gtgtggtaat gctggaactt tcgccctgta attcatcatc
     7441 tcggcttaac ctaagataaa gtgcagtatt gtaaatctgt tgtttcattg tcagttatcc
     7501 tcctttataa ctaacccgtg tttacaatac ctgcttgcaa gtaaagtata acacgggtac
     7561 tttttgtcat tcaatctatt tcttacaact gcaccgattt tatgcgattg tctgctttgc
     7621 tttttccagc atgacatcag ttagaagttc ttccattgtt ttgccctttt cggaaaaatg
     7681 ctcttttact acaatctttg tctttcctct gttgccaatg ccacacttgc catttttctt
     7741 ttgaattgtt tttacagcat tatcaataac acataccccc tttctgtaaa atctttctaa
     7801 gcaattcaac ggcttttgtc atagcccaca ggagttccac ctctgcatag ttcttatgta
     7861 gccgtaccca ttgcgtgcga cgctcttaat gctcaagctg tggctgtacg ggagtatcat
     7921 tatctgacag tacaggtcat ggcgataaaa gaggacaaat cttttacaag aacacaaata
     7981 ggttttcgct cgctcttgca ggggaaaggt cgtggcgtat ttgtttcaac ggctagctgt
     8041 ctgtcaaacg tattgaatag ctttattcag ttgtcaaaga acgattgaaa gaaaaaaatc
     8101 gtctttccta tatatcgcgt tggaaaagag gagaaacgta accaaaatcc aaaacttttt
     8161 ccattattag tacaaatgga acggttcatt ttggcaatca acggttgatt ttctcctttt
     8221 tcactactat ctgtacagca aacgtccatt tgctaagttt aaatggaaaa attttaactt
     8281 cttttcatgc accatatagg gatagaaaat gtcattttgc taagttgatg atgaaaaaag
     8341 aatattttcc ttttcacacc ctaactaccc aaaccatacc gttactgcca acaatagaat
     8401 gaaatatttt ctttgcacca tatagggaac ggaaacctta aattgttaag ttgagaacaa
     8461 agtattttca tcttttcaca tcaacactca actgctaatg cctgtttgaa tacaaaatga
     8521 tgatgttttt cctcttttca cagaagactg tccactaatc acgaatttcc gtcatttttt
     8581 caaaactttt ttcttctttc acttcatata gggacagcaa gccccgattt aatgacctat
     8641 tttcaaaaaa agtttagatt tccttttctc gcaccatatc tgtacgcaag tagccatttt
     8701 gttgcagatt taataaaaaa tttatttttc ttctttcact tcacataggg aatgaaaaca
     8761 gctctttgct gactaaatct gaaatttttc aattacatta aaaaataccg tcattctctt
     8821 gtggaacagc ggtatctata cgttcttcta tggttgattt ctctaattgt aataaatgtt
     8881 tatgcttgtc ttttagttct gcctgctcaa tcatatcttt cagtatcgtt gtacgatttt
     8941 ctctgtctaa tgcctgtacc atttttatgg ttggtgcgac ttgtctttgt agccaatgca
     9001 acgctttttg taaagtgtaa ggctctggct ttgtggttaa gcgtatcggc tctctgtttt
     9061 cccctacaaa ccatgcccaa tcatcatttg ttatccattg actttttggc ttgtcgtcct
     9121 ctctgtcaac aaagcggaca taatgattga tgatagaaaa agcggtgcgt tcagcgtccc
     9181 gataggtcag taaatctaca accgcataat aggctcgttc gttcttcatg cgtatttcaa
     9241 aacggttctt aatctccgta tcttcaattt ctgtgccatt cttgacatac tgctcataat
     9301 ttttctgata agcacacata tataattcgc tacttgttga accgagatat aaggtttctc
     9361 ctgtattctt tggtaaatcg ctaccgcatt tttctgtacc gctgtaatct ttctgcatac
     9421 ggaagtagga catacattcg ccagccttgt atttttcctt taactttgga atatccaata
     9481 ttcctgcttt atcattgata gccaaatcaa gccgtttcat cacaccacca gccgtcatac
     9541 aatccagcat gaagtcatac catgagcgtt cctgtgccag cagataacat tccatttgcc
     9601 gacagccttt gcctttcagt tccaataaaa cacctaaatg ctcctgcatg gaacacatga
     9661 tattgatgtc gccaagtaca tatttgcttt catatccgta ttttccataa tcttcataaa
     9721 gcatgtaatt agatttcagt tgtaatacat cacggataat cgccaatgcg tccgtagtag
     9781 gaaagcggac acgaaagtaa tcaatcaata agaatagtgg ttcttgcgga ttgaaacgct
     9841 caatctgttt ttctatgatt tctcttaatt cgtcattcaa tggctgtttt cctttttcaa
     9901 tgcggttgta atattcacgg ctgataccac aggcaacagc aagccttgtt tgtgttactc
     9961 cgtattcctc acgcttttgt cttagttctt caataaagtt tttatcattc attcttcaat
    10021 tcctccaatc aaaaaaggca actaccataa ttgataattg cctgtcatta aaaatctgta
    10081 tagaaaaagc agtcaatcct aagactaact gctttgtgtg tatggatata aaattgcttc
    10141 taatcttgaa taggtatcca aatttcaata actgaattat cactattcca atgaaaacga
    10201 taatcgtatt tttcaaagtg taggataata tcttttatag gaatatacga tgtactagga
    10261 ataatttctt cataaatctt gccataagtt tcatatattt tatccatagc accaatatgc
    10321 tccacaacca aatatttata agacggtatt tctttataca gaaagccctc tggggtaatg
    10381 gttctttggg gaatagaaca gaaataataa agttttccat ttcttctttc cataaaagca
    10441 tattttaccc aatttcctga ttgtgaaaga tacgcttttt tcaaactact gttaaacttt
    10501 ctccaaaact gtgtactaat ctgaatattc tttttttgat aattagtcag ttctacttcc
    10561 tgcccaataa ctgcaaatgc gtttaattcc tttatagaaa aatttatcat tttaccttgt
    10621 gagcagtgat aacggtataa ctgtacgcat ttaccgttat aatacaatta tcaatcgtta
    10681 tataccagtt ttttcctttt cttgtgattt ccgcattaga aaggctaatt ttttctttgc
    10741 accattcaat tacattttct acattcaaag ataagttctt tttaattcta ataacgccaa
    10801 gttcagttgt gtgaagtctt cctaagtttt ccagtaattc attttccata ttttgatacc
    10861 tcatttttca gcaaatttat ttgtatgttt tataccccaa ttataacatt ctcatatcag
    10921 cactacaata aaaataatta agcaccaata atcttattga acatctccaa ccgtaaatct
    10981 ttcacaccat cagtgataaa cactgagctg acagcaatta cgacaactac cataattgcc
    11041 tgcacttaaa tttctgcaaa agaaaacgac aatcaatttc ttattttttg aaatcaactg
    11101 tctttctcat atttactatt caattttcat agtgatatag gtacaattta tatcactact
    11161 gtaaaaatgg ggttttgcct tataaataca gcgttttccg cttgttagtg tatagtgttt
    11221 cttgtttttt gtgcccccct tgttagataa cgggggcttt gaacggctcg caagctcgcc
    11281 gaaccgccga agcgaaccgc ccccttgcaa gcatggcgga aaaatcttaa ggattttccc
    11341 gccaacgtgg tcggatcgct ccgtcggttt aacagctttt ctgtttctct ctagttatct
    11401 acgcatataa atcaaatcgg ttattccgtt ataagtctgt gttttacaaa gccttaactt
    11461 tatttccctg tctatgttgc ccaaaagacg aaccccattt cctaacagag taggaataac
    11521 agaaatgtaa tatttatcaa tcatattttt gcttaccaac tgctgaacaa gatttgctcc
    11581 gccacaaatc caaatatcct ttcctgcttc ttgttttagc cgttccaata ataaaacagg
    11641 attttcactt gtaaaatgaa tttgttggga agaattgcac tctttatgtg ttaatacata
    11701 agttgtaaat ttattatata cccattcttg cggagaaagt tcagtaacaa cctgatgaga
    11761 agtattccac cccattaaga cagtatctat atctttggta aattctgtat aagtgtcgat
    11821 gttttcattg tcatttccat gaccatttag ccaagctact ccaccattac cgtccgcaat
    11881 atatccgtca aggctcatgg caataaataa aactactttt ctcatttttt ctcttttata
    11941 aatacaaaca ggaatttatt tctctttttt actcataagg gaacaactga ttgctacaaa
    12001 taaacccatt aaaatccatg gaaatgctgc tcttataaaa tcactaacca tactttattc
    12061 ctccttaaat tccaatttcc ttagttctac aaactgaaat ttttgcctat gaatgtatct
    12121 tgttctttat acgcaaacag gcttctgtgt gcataatcca atgtcttgaa aacggcattt
    12181 gaattagtcc acaaatatct ttattacacc aataatcaat cagccaaatt gctttttcat
    12241 tgtcgtttac tacattcaac gattctaagt atccttttct ttctttcggt atttttcttt
    12301 tcaattcatc ataagataac cgttcaagca ttttctcagt gctttcccat acttcgaaaa
    12361 tatataaata taattcttcc agattaagct gttttgaaaa gtctgcaatc tgctgtttca
    12421 tgagttcatt tcctgttgta atgataggtg aattgatacg ctcttgataa ttgcctgcaa
    12481 aaaatacttg ttcatcttca tttatcactg tatgcacaac tatatcttca atgcggaaaa
    12541 tatgccaaat agaataagca atcgtcttac tatgataacc atcagcgttt atgaagggga
    12601 ttgagttaaa atcttctctg cataattctt ctttgaaaga tactaaggtt tgtattaact
    12661 gacttcgtaa agtaagtaaa gtatcaatac ccctcttata agtatctttc ttttttattt
    12721 gtgcctgcat tgttttatta agttcagacc attccttatt catattcaat cctctctcaa
    12781 attctgattt tatcgcccca tacaaactgg aatttgtctt acaacaattt atatatgcat
    12841 aaaaagcaat atatttatga aaacacccta aatccaaaat ttactcaatt cattttttcc
    12901 ttacataaaa tgttctcgta tatttcactt cctcaccaag tgtttcccct atcaaaactt
    12961 caaaatcatt gatatcaaat tctggaaaga atgtatctcc atcttcaata tttaaatcta
    13021 cttctgtgat atacatttta tctactattt gcaaagcttc cttaaatagt ccatatccac
    13081 cagatatgta tacatctcgt cctttagcca ataataatgc atcttctaat gatttaactg
    13141 aaactaaatt atctccttga tactctgttg tggtggaaac aacaatattc attctattag
    13201 gcaacggatg accgatttct tcataagact ttcgccccat aataaccaca ttacccgttg
    13261 ttaactctct aaattgcttt tgttctccct ttattttcca tggtatatta ccattcttgc
    13321 ctataacatt attctttgac ctcgcaacaa tcaaacctat catctatttc atcctccata
    13381 atatgatgtc gtacctgtga ttccatcaag tgcaccacat cttaacaaac tcaaaattat
    13441 ctcgaactaa ccagctcgac aaattccttt tttgtcttca tctgcattat atcatattgt
    13501 tataagcatt gccatttctt ttctgctttt gaaaagttac ttatcagcct atgttttgta
    13561 aagggtgata ttcagcattt ctcacgcaac aagcccttga caaaccatag actaataagt
    13621 tttaaggtaa tcaagcagaa atgaaatagt ctgctgtttc acggcaacaa aaaagagata
    13681 ccaacacact tatgtagtat ctcttagtct agtagcaata ttcttttgtt tgcaagcagg
    13741 taaacatggg ctgtttctta atatcttttt gtttgggaaa cacctttttc ccatcctttc
    13801 accgttgcca tctctgctgc gcacaatgcc acagggataa accatagcaa accgcctaat
    13861 aacaagaaaa atactaagct gaagcctgag gtcgcgaatg tgggatattc atataatgaa
    13921 acgaccattg agatggtcat agccaaaaag ccaaagaacg ttaatttttt tgcaggaatt
    13981 ttcgttttgc tttgttgcat gaatcttcct cttttctatt tgaatttgtt tcaattgagt
    14041 ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt cttcttttgt agtagtgatc
    14101 gttgtaaagt gcatcgccga ttttaaaatc actgttgtca gcacataggc atacacggca
    14161 gtaatgatga caccaatcac ttgaatccct aagaagtacc agctgcttgg attcgttagt
    14221 aaatggctgt tagatgcaaa gatccctatc agaatcgtac ctgtgaatcc gcccatgcca
    14281 tgaacacccc acacatcaag tgcatcatcc cattgtttct ttttacgaaa atcaacagca
    14341 gcatgacaga cgatacccgc aatgatccca atcagcatgg cactttttgc ttctacataa
    14401 ccggcacaag gcgtaatggc tgccaatcca gcaacggatc ctgttagtac gtcgacaaaa
    14461 tcaaagccat ttccttttac ttttgcaatg atcagccaag taatcatcgc aaatgcaaga
    14521 gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat ctgctcgaag tgcaccaccg
    14581 gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac cgatagcagc tgccataaga
    14641 ctgctatgct gacttctttc tcctaaagga atctttcttt gaccaagtgt gaggatacat
    14701 gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc caccagcgaa atctctaaat
    14761 cccatctgat ccaaaaatcc gccaccccat atccagtggc aaacagggaa ataaatcagc
    14821 agattccaaa aaataacaag tataatatag ccttttaaat tcaatctccc tgcaaaagct
    14881 cctgtcatca aaggaacggt aatcacgata aacatcagtt ggtaaagaaa gaacattaaa
    14941 aatggaatcg ttgcaccatg aaacatgttg gggaagcttg taacatgtct catcaaaaag
    15001 aaatctgccg ggtttccaat aacaccgccg atatcccgtc caaaggccaa tccgaatcca
    15061 ccaaataccc acataatcgt tgttatccca acggagagaa aagattgaga catgatcgtt
    15121 agtatattat tcttccgtgc taatccgccg taaaaaaaag ctagaccagg agtcatcagg
    15181 caaaccattg ctgtacataa aatcatgaat gctgtatctc ctgtattgat aggtaatgta
    15241 ttcatttttt tcgcctcctt tctacgatta cgagtataaa agaaggtgta gttgtttttt
    15301 tcttctgttt ttttgaatta atacttgcat ttataaaaga cagacaatta ataaaagctt
    15361 gatgtgttgt gctctttttc gtttttcaca caaataaaga caaaaaataa acggtcttct
    15421 ttttatagaa aaccgtttat tttcgtcttt ttttattttt aaagatattt tttcataagg
    15481 aataaagaat acttcatata aaatatataa aaaagaaccg cagcgagtgt cgctacggtt
    15541 ctcaaatt
//
LOCUS       DACBZN010000063        15471 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.063, whole genome shotgun sequence.
ACCESSION   DACBZN010000063 DACBZN010000000
VERSION     DACBZN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.063"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..1126)
                     /locus_tag="IVD64_001843"
     CDS             complement(<1..1126)
                     /locus_tag="IVD64_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6149094.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1209..1544)
                     /locus_tag="IVD64_001844"
     CDS             complement(1209..1544)
                     /locus_tag="IVD64_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6149095.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1554..1928)
                     /locus_tag="IVD64_001845"
     CDS             complement(1554..1928)
                     /locus_tag="IVD64_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6149096.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1941..2255)
                     /locus_tag="IVD64_001846"
     CDS             complement(1941..2255)
                     /locus_tag="IVD64_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6149097.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2368..5595)
                     /gene="fss3"
                     /locus_tag="IVD64_001847"
     CDS             complement(2368..5595)
                     /gene="fss3"
                     /locus_tag="IVD64_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6149098.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPEVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(6009..7175)
                     /locus_tag="IVD64_001848"
     CDS             complement(6009..7175)
                     /locus_tag="IVD64_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6149099.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            7200..7526
                     /locus_tag="IVD64_001849"
     CDS             7200..7526
                     /locus_tag="IVD64_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149100.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            7679..8731
                     /locus_tag="IVD64_001850"
     CDS             7679..8731
                     /locus_tag="IVD64_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6149101.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            8807..9025
                     /locus_tag="IVD64_001851"
     CDS             8807..9025
                     /locus_tag="IVD64_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149102.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            9012..11585
                     /locus_tag="IVD64_001852"
     CDS             9012..11585
                     /locus_tag="IVD64_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149103.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKF
                     DSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSC
                     N"
     gene            11576..11773
                     /locus_tag="IVD64_001853"
     CDS             11576..11773
                     /locus_tag="IVD64_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149104.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            11777..12388
                     /gene="mobC"
                     /locus_tag="IVD64_001854"
     CDS             11777..12388
                     /gene="mobC"
                     /locus_tag="IVD64_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP6149105.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            12373..13764
                     /locus_tag="IVD64_001855"
     CDS             12373..13764
                     /locus_tag="IVD64_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6149106.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            13980..14294
                     /locus_tag="IVD64_001856"
     CDS             13980..14294
                     /locus_tag="IVD64_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149107.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            14287..14781
                     /locus_tag="IVD64_001857"
     CDS             14287..14781
                     /locus_tag="IVD64_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149108.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            14849..15163
                     /locus_tag="IVD64_001858"
     CDS             14849..15163
                     /locus_tag="IVD64_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149109.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            15262..>15471
                     /locus_tag="IVD64_001859"
     CDS             15262..>15471
                     /locus_tag="IVD64_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6149110.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         5204 a         2601 c         2782 g         4884 t
ORIGIN      
        1 gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc aaaatcagaa
       61 agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa atcaccgcac
      121 tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt aaaaagttgg
      181 gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt tctttcattg
      241 ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa atttcttcct
      301 tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca tttttgggat
      361 cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg aggaggaaat
      421 aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa tcataagccc
      481 atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg tctatcccaa
      541 ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct ttgagttcct
      601 tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt aaaagctgat
      661 cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct ttcattcgat
      721 aatagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc aaatctttga
      781 aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga atcatccgag
      841 ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga atataaagca
      901 atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct tttatagacc
      961 aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta aactcttgaa
     1021 aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga acaaagtgat
     1081 aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg atggtttcca
     1141 cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt tatgctagtc
     1201 atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa aaagccttca
     1261 atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg ccgacataag
     1321 ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca gtctagtaca
     1381 tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat aatcagaaac
     1441 attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc gccttttcca
     1501 tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt cacctatttc
     1561 tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa gactaaatca
     1621 tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt ggcaacggta
     1681 tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg taagggtaca
     1741 ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa attataggtt
     1801 cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac ttctcggcgt
     1861 aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat accttctgtt
     1921 aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa cacgtaatca
     1981 gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt cacggctttt
     2041 agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac cacctcaata
     2101 tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac cgtagtattc
     2161 cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt tccaaaggtt
     2221 tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt tcattgttag
     2281 ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac gttggttctt
     2341 aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat aatagcccgc
     2401 cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt tctccccggt
     2461 ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa cagttggtgt
     2521 ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta aggtttgaga
     2581 tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat cgtaattgat
     2641 ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt ttttcgcaag
     2701 cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag atttccaaat
     2761 ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag cttcttttga
     2821 gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca catcaccatg
     2881 agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa catggtgttc
     2941 cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg ctactggctt
     3001 atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga caatagtttt
     3061 atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg ttttctcttt
     3121 gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt catctttttc
     3181 ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc agtcttcttt
     3241 gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca gagaagttaa
     3301 ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt cctcaggcaa
     3361 atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca gcttctcgta
     3421 aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg taaagacata
     3481 ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt ctaatggcgt
     3541 aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc cataaggaag
     3601 attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg tggtcgcttc
     3661 atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa aggtcagatc
     3721 attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga aatcaaagcc
     3781 aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat ttttttcgct
     3841 atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat atccttcggg
     3901 cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg caacttggtt
     3961 cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct tcgctaactc
     4021 tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa acaaggtgta
     4081 ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt tgtcttcttt
     4141 cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac tggtaacaag
     4201 ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg tattcacgaa
     4261 cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg cattagcaat
     4321 ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa cgatttcacc
     4381 ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat tgtcattcca
     4441 aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt tttcccgtgc
     4501 gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca tgggggtggt
     4561 atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg taccatgagg
     4621 aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg caggtaaagt
     4681 ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt ctttatcgac
     4741 ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt aggtattagg
     4801 tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg cgacaggtgt
     4861 tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat tggcatttgg
     4921 ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat tttccacaac
     4981 ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg attgccctaa
     5041 tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt caatggcttg
     5101 atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc gttttattga
     5161 gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa tatgagtatc
     5221 tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat cggacatatc
     5281 tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga cttctggttc
     5341 aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa cagtgacttc
     5401 tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg ctaaatctag
     5461 ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg taggagcaat
     5521 tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata atttcttttt
     5581 ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat aagatgctcg
     5641 cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat agaatggatt
     5701 tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa atatactgcc tttaaattag
     5761 aacacatttt agaacacaaa catttcaaat ttaatcaaat ataataaaat atatttacgg
     5821 gaaaaccttt ataaatcaag gtttttcaaa cttattcaat gatacgaaga aaccaggtct
     5881 taagaaagca cgtaaagctt cacagttttc aaaacgttaa tcgttgttat accaacgatt
     5941 tcagacactt tctcattgcg agaaggtgtc tttttttcgt tctggtaatg agaaaggtaa
     6001 tgacaatatt ataaaccagt gtaagcaatg aatttttcta acgcatttcg ttgagctttt
     6061 tcagtaacat gagaataagt gttaagggta gtttcagcat ttttatgacc caatcgatct
     6121 tgtgtttctt tgatacttgc tccaccttct agtagaagag tagcgtgagt gtgccggaat
     6181 ccatgaatag taacgtgctt aaatttatat ttcttctcta aacgtttcaa tatattattt
     6241 gaatagtccg catgcaaagg ttgattaatg tcaccgtctg catttacata ggtaaagata
     6301 agttgatttt ttgtatttgt atttatgcca aactgcaaaa gctctcgagc ttgttctttt
     6361 ttccagattt ttaacatttc aatagtttgt gcatcaattg gtaatattcg ttcggaagat
     6421 atattttttg tatttttttg aatcctgaca gtttttcttc ttgcattctt ctcttcattt
     6481 cgaatagttg catagctctt tctaacatac attagctgtt cttcaaaata aatgtcagac
     6541 caatgaagag cagctagctc tcctttacgt ataccagtag taatcaaaag ctggaagatt
     6601 aaaatatgtt ttaaaggctc ttctttattg agaatatccc aaaaagcttt tagttcttct
     6661 aatgaaaagt aattttcatt ttctttcttt tcccgctttg gtagatttgt atttgccata
     6721 ggatttcggt gtaatagctc tgcaaaaatg gcatactcaa atatttgaga gctgtaagac
     6781 ttaagctgtt tgatattctt gaattttaaa gcccattcat tgactgcatt ctgacagtga
     6841 acaggagtaa tcttagaaat ggacttattc ccgaattttg gtagtatatg caagcggaaa
     6901 aggtcttttg tacgttgata tgtcgtttct tgtacacgat tcctataaga aggcaaccat
     6961 tcttcataca ctgttataaa caagtcatta gaaggcttat gatagccggt atcttcaatc
     7021 tcgacctttt ttcttgcttc gtgtaacttg gcttcttttt cagttttaaa cccttttttt
     7081 acatagtatt cttgctttcc attttcatct ttacgaacat acaagcggac actatatcgt
     7141 gcaccgcttt ttgttgtgta cttttgtatt gacataacta atctctccaa tctgataact
     7201 tggcgagttg gctgattgaa gaaatagaaa atgagaggag aaaaataatg gatgcaggaa
     7261 ttacagtaat gttagataaa caacaaacga atcaattgaa aaattatgtt tttgaaatga
     7321 caaaagaggc tattgatcaa gcaagaattg ataccggatt agaaagacca tttctaaaag
     7381 gaaaagagat ggctaaatat ttgaatgtat catatacaac ttttttgaaa tttaaaagaa
     7441 tgggattgcc agttatttta ttagaaaaaa tggagttgtt ttcaaaagaa gaatgcaaaa
     7501 aatggatatt gagtcatcaa atttaaaaaa cacctcgttt tttaaatttg atgaagtaga
     7561 aaaaattaaa aagagtgttc ccgtcagttt gcagatcggg ggaacactca ataataaagt
     7621 tctgagaaaa ctttatttga atctattata gcaaataaaa tcagaagcag gtgtgacaat
     7681 gaatgaaaaa gaagttgaaa gcttagttga aaagcaaggc tattctaatt ctgatatata
     7741 tgcagaatta tactatcaat atcctatttt tttaaacgta aatgaaaaat ataagaaaat
     7801 gaaactaagt tctaagttag tttatatgtt actaaaagat agaaatagac tttcattgaa
     7861 aaataacttg tatgatgaga atcaaaaaat ttttttgata tttgaaatca aaaatctcgt
     7921 agaaatttcg ggaatgtcaa aaaatacaat cttatcagca ctagatgaac ttcaaaaagt
     7981 taaacttctt gtgaaaagaa aaaatggctt caataaaagt aaaatgcaaa tgttcccaaa
     8041 tttttattat ttgttaaagc ctgttctgtg caaagatgat gtttataaaa ttaataagat
     8101 aacccatcaa gaaaatagac aaaaaaactt gatagttcaa aatttgaact atggaacaaa
     8161 tcaatattct gttgatagtt caaaatttga actatgcgac acatcaatga aaacaagaga
     8221 caaaaacaat tttgctgata gttcaaaaaa tgaactagaa ttatataagt cttataaaga
     8281 tagtaaagat attaaagaat cacataatga tgatcttctt gaatcacttt caactaaaaa
     8341 taataaatta gatgaagaat tgatcgaaac atttattgaa gaaaatcttc ttaaacaaat
     8401 atggggagaa agtattgtga attgcttaaa actggctagc aatagtgatt atcgtcaatt
     8461 cgacaactgg tacaaaaaat tcaaatatgc gattagatct gctgaaaaag aacaaaaaat
     8521 tcaactgaaa attatctatg aaatttgtaa taacaagtat tttgtagatc atgttagaga
     8581 acagctatca atgactttac gtgacttgat acgaagagca aaaacagatc atcgtatcaa
     8641 acaaaaagac gattatatct ttgcttctct aaaaaataaa gcgttagaac taattgaatt
     8701 gcaaatcagc gaaggaatag ataagcaata agaatagaat tgagtcaaat gaaatggagg
     8761 ggtatctcca cttcatttga gaactttgaa tataggaggg gttattttga ttaaaaatcg
     8821 tttaatagat caagtttggt gtctagatca aaatacagat agtctcgaat taatcgagac
     8881 tatttgcttc aacactatag ttctcgattt aagagctgaa aatgataatt gtgtaactca
     8941 tgtcataatt aatcaaaaaa tggctcagaa gctctctgag agcctcagaa aatgggctaa
     9001 gggaggtaac gatgaaaata attgaaaaaa agattgccaa taaaaaaata aattggttgg
     9061 ttgttaattt tcttgttctg atagctatga ttatttttac aagtaatatt gctcatgcga
     9121 gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat aattctcttt
     9181 atagacctta cctaaaaaag tacaatggag atttccttag tcagttcggg atttggctcg
     9241 gatgggctgt ggtaaaaggc atgtttactg tgacagattc gattcaaaat atgattccag
     9301 atgtgctgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt tatcaatcag
     9361 ccgtaaatac aattgtcatt ggattaatga tcctttcatt gatgtttgtt ggttacaaaa
     9421 tgattactgg aaaaggaaca gtggatttaa aatcggttgg aatgaacgta gtgatgtcta
     9481 tagcattaat acttttaatg ccaaccatga tttctagtgg tattcaattt gctaaaacat
     9541 tttataagga ctctactacg attacaaatt cagatgacgg agtcgcttgg tcattgataa
     9601 aacaaggagt aacagattta gcatatatta ataaaacaga tcagtactca agcattgata
     9661 aaacggagga tcgaaataat ttaacacgaa aaaattttca acagacggat ctcacacaag
     9721 tgttaacgga taaagtgatt gataaattgg aaaaagaaaa tccaattgcg gacaatcttc
     9781 gttatgaatt agttgaaaat tctaataatg aatttgttgc tacaaagttt tctgacaact
     9841 tcttatcaac attttctgat agcttgaaat caggctatta ccgataccaa gcaaatctat
     9901 gggggatttc aattgggttg acagcgttag caatcgccta tgtgttcagc gcgtttgtga
     9961 tcattacagc aatactagaa cttgcattta aacgtgtgtt aggtgttctt gtttttgcaa
    10021 cagatattga gacaggacag cgttcaaaag ttgttctatc agatattcta caatgttact
    10081 taactgttgg gtttcaagga tttgggttat caatgtttgc tatgtttatc aattttctga
    10141 actcaggaca aggtatctca acaaatattt ttataaaaac aattgcttat atctgtgccg
    10201 tttttgtttt aatcaaaggc agtggaacag tcatgagata ctttggtgtt gatattggac
    10261 tcaaagaagg ctatgggcaa cttgcttctg cttttggaat gggcgcaatg ctgtttagaa
    10321 aaggatcaaa tggatttaat cgtgctaaag gaagcggaga aggagaagat cgaaaaccag
    10381 aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa aactggaaga gctttgggat
    10441 atgcccatga acgtggatta tccggtttag cttcagatgg agctacaatg gctagtgaaa
    10501 gagcaacaaa accatttaag agtatgcgtg atatagctaa tgataccaag aataagttta
    10561 aagaaggttt agatgatggt acagtgtccg ctattaataa aaatagcaaa cctatgttag
    10621 ctaaaaataa agaagatgaa actggaaaat atgctgattc cgtgccaaca cgtctttcag
    10681 atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat tatgtctagt tctgaacgta
    10741 tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa tcctgcaagc gcaattcagc
    10801 aaaaagtcca acaagatatt gaagaacgta aaaatgctat gcacggtcaa gctaagtctg
    10861 ctgaagaatt aattaatcag aagcgtcatg aagcgaaata cacgcctgaa gcaatgaatc
    10921 gcgaggaaat aatgttaagg aaacgtgtag aagggcggac aggcgctaat attgacggaa
    10981 aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa ttcaaataca ggattagaaa
    11041 aattagtcaa agaaaatcca caaaattcta caagtagtca tggtggccga tcagtagatg
    11101 tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg acggaatgtt gatgtaagag
    11161 agaatttaca aagttcttca aatgctcaag gtactaagac aattgatgtt cgagaaaatc
    11221 tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt tcgagaaaat gtacaaaatt
    11281 ctacaagtag tcaaggattc aaaacagtag atgttcggga gaacattcaa aaagatagtg
    11341 gagaaatgaa agaaaaaaca caaaaaatca atatcgttga agacagaaag tcagcgaaaa
    11401 aatttgattc aaatcatgaa acagtgatta ttgatagtga aatccgtgaa aatgataagg
    11461 gatcaaaatc aagacgaaga ttcacttatg aagacaatga gttattcaga gatacactta
    11521 atgatcctaa tccacttttc gataatttat taagaaataa aaggggggga ttgtcatgca
    11581 attaattcta aagattagta cgcatgattt cttagaactt gacgaattat gtgaatcagt
    11641 aaagaacgca acaaatgaaa aaatcacggt cactaattat attgaactga tgattcaaga
    11701 agaattaaag atgaaaaatg aaatagagag caatttcaaa tgttgctctc tagaaaaaaa
    11761 tgtgagggga taatcaatgg atgataaaca aaaaatgtac gaaaaaaatc gcaaacatag
    11821 tgaggcctta aattttcgtg tcgataaaaa gacttatctt gaactgacat tgattgcaaa
    11881 acaaagggga atttctagag cagacgtttg ccgtgagcgg ttaaagggac tcaaatatga
    11941 accgccgttg ttagctgaag atgatagggt agttttaacc aaacagttaa atcaaattgg
    12001 taacaatttg aatcaagcgg taaaggcatt aaatatcatt gctaaatatt ttagagaaga
    12061 atctgaagat aaaaaagaaa agagcctaca ttttagtttt gatgctagcc gttacaacat
    12121 ttttaaagat gaacttgcga atagcatagc aaaaaagttg atgattagtt atccagatga
    12181 catttttttg gagtccaatc ggagtgcccc ctcttttttt aaattatcag aagtagaaaa
    12241 agaatatttc gaatattgga tagaaaagca aactagaccg ggtacgaaag aagagtattc
    12301 ccaaaaagtt gatcggaatt taattcactc aattcagatt attgcaagtt taaaagatga
    12361 ggtgaaacaa ctttgggagc tactataaca tcgaaaggac gcaagaaaaa tttatcctcg
    12421 ctattacaat atgcagctaa tccttcaaaa aaagaaaaat gtgttgcagt ctcaggaata
    12481 aattgtagtg atgatagtga ggaagcactg caagaaatga atttagtacg taccaagatg
    12541 aataaatcta aagatatagc acaagcgttt catgttattc attcgtttga taagagtact
    12601 tctaaagagt taagtattga taaaatgcac gaaatagctg tcgaatttgc agagaaagct
    12661 tttccaaatt cgcaaatcat tgttgcaagt cacaatgata aagatcattt tcatagtcat
    12721 cttgtcatta ataatattaa catggaaaca tgtaaaaggt tgcgtattga ccctaaagat
    12781 ttagaactaa tgcgaaaaat taacgatcaa gtcctcaatg aacatggtct tgaatcagtg
    12841 aataatgagt actatgaaaa cttagaaaag agaaccaatc tttattcaaa cgatccttct
    12901 aagatgaaat atggaaaaat taattatcaa gagtttgtgc aaaatgcgat cgaagcaacg
    12961 ttggaaaaat gcgaagtgaa ttcttttgaa gatttttcgg atcatttggc aaaagaatat
    13021 aacattgaag tttatcaatt ttcaaaaaca aataacaaac ttggctatgt tctttacaaa
    13081 caaggtgtaa atttctatga gcgtagaggt gagttaatta ctttaggaag agataaacaa
    13141 tctagaaaag aaaaaagtca gtatgtcgaa cgtactttta gtgcaagaaa gttagggaaa
    13201 agatattcat ttgaatctgt ttctgaacaa cttgaaaaga atttagaaga atcacatata
    13261 aaaaattttt ttaatatgga aagagagcaa gttatgtcgc taattagtgg agtaaattac
    13321 aaaactggtg agttaaatgt accgtttgtg gaattagatt ccatcgcaca aaagcaattt
    13381 agcgaccgtg aaggcgatga aaaattcatt aaatatgatg acttcttgcg agaggtagac
    13441 aagcattttg taaatacaat gtctttagaa gatgatcaag aatacacgat taaacttcat
    13501 acggatgtga atcctcaaac tttacgtagt gcgaaaagta aaacaatttc tattaaaaag
    13561 actgatcaga aagagttatt tctaccggca aagggttttg aaaattgttc tcatacacct
    13621 tctgctaaac agattcacta tcaaatcagt cgcataatga atgacgatgt aaaaatgtat
    13681 cgtgctaaaa aggattatga attcacacaa caaatgattg aaaatcagca aaaaaggcaa
    13741 caaaattttg aaatggaatt ctgaatgggc ttgttagtgg actttccagt tttttgccta
    13801 tgaagagttg cttggaaaga agcagttttt aggacatacg ccagctaccc atctacttgc
    13861 cagtcaatcc gccccgctca cgctaggcta ctttctggca agtcagatgg cgggtaaatc
    13921 tctgagggag caagctccct acagactccc tcccaaaaaa agaaaggtgg aagaaaaaaa
    13981 tgaactatcc atattttaaa gtctcggctt cagaagaaac taaagaaata ttcaacaatt
    14041 tttacaacca aaacaaagga atttttggtt caaaggcaaa tatgtttcgt gttatggttt
    14101 caaatttacc agtgttagct agtccatcaa ataataaatt taatgatcct gaaagcatta
    14161 aatttgaaca aaaaatttca gaactagaat caatgataag taatgaagta attgaaaagc
    14221 tggatgacat tgatcaaaag ttatcgtatt ctctaaaaaa caaatacaaa acggaggaga
    14281 aaaaagatgt ttaaagttta cttcccgttt cagtggctcg ttaaaggaat tcttttagtg
    14341 ctactaggat tgattctttt tggaagttat ctaacaatta aagccagaca ttttccagaa
    14401 gaaaagccaa taaaaattgt aacaaaagat ttaaaaatag gtgaaactta tcaggcagat
    14461 ggggttgaac taacatttaa aaatattgaa gtgaccgaaa aaagaaatga atttgcacaa
    14521 catgatcgtg tattacaagt gacctataca cttataaatg atacagaaaa tccgatacgt
    14581 ccacaacaat ggtcgattga aaataaggat ggtgaagaac tttatgagta tgatatgata
    14641 ttggataatc cagagtatat tgaaagtggc aaagaagaaa cagtaaatta ctattatgaa
    14701 ttaccgattg aaaggcagga ctaccaaatc gtagtacgta gagagaatgg ttacatagtt
    14761 gggttgccat ttactttata agttttattt tgttagtagt ttttaaggta atatcagttt
    14821 tgtgattttt gaaaatggaa ctgggagaat ggctgaggaa aataaagttg ttccgtttga
    14881 aacattagtg ataaataagg aagagtttga gcaacttttt cttgattttt ttattcaata
    14941 tgacatgatt tatgatttta gttcgtcact agatttattt gaattagaag cagaaataat
    15001 agaaaacagt cgagagaaga atggtaaaat cattatttca gtagaaaaat tcaatgaatt
    15061 attctcacaa acatttgaaa tagatccaga ctattatcaa ccacaagaag tttttcgaga
    15121 gtttagaaat aagattattg gttttagtca aactacaaaa tgaaagctta ataaagcaag
    15181 tgttgataga attattcctc aacgcttgct ttattttatg ggcacttcat aatagaggta
    15241 tagagaagag aggtattcat tttggatact attactccaa caaacttcaa agaaaatata
    15301 ggtaaagtgt tgcaagaagt agttcattca aattctgaaa tagaaattac tagggattct
    15361 aaaaatggat tgaacgatgg cgtcattatg atttctaaga gaaaatggga gttgttacaa
    15421 gaagaacttt atttacaacg aacgggtaca tgggcttatg tatctgattt a
//
LOCUS       DACBZN010000064        15418 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.064, whole genome shotgun sequence.
ACCESSION   DACBZN010000064 DACBZN010000000
VERSION     DACBZN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.064"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(47..931)
                     /locus_tag="IVD64_001860"
     CDS             complement(47..931)
                     /locus_tag="IVD64_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6149111.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(1709..2995)
                     /locus_tag="IVD64_001861"
     CDS             complement(1709..2995)
                     /locus_tag="IVD64_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6149112.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(2988..4256)
                     /locus_tag="IVD64_001862"
     CDS             complement(2988..4256)
                     /locus_tag="IVD64_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6149113.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            4508..4969
                     /gene="mscL"
                     /locus_tag="IVD64_001863"
     CDS             4508..4969
                     /gene="mscL"
                     /locus_tag="IVD64_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP6149114.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5028..5474)
                     /locus_tag="IVD64_001864"
     CDS             complement(5028..5474)
                     /locus_tag="IVD64_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6149115.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(5567..5812)
                     /locus_tag="IVD64_001865"
     CDS             complement(5567..5812)
                     /locus_tag="IVD64_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149116.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(5926..6504)
                     /locus_tag="IVD64_001866"
     CDS             complement(5926..6504)
                     /locus_tag="IVD64_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6149117.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(6581..7111)
                     /locus_tag="IVD64_001867"
     CDS             complement(6581..7111)
                     /locus_tag="IVD64_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP6149118.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(7115..7975)
                     /gene="rlmB"
                     /locus_tag="IVD64_001868"
     CDS             complement(7115..7975)
                     /gene="rlmB"
                     /locus_tag="IVD64_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP6149119.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(7968..8363)
                     /locus_tag="IVD64_001869"
     CDS             complement(7968..8363)
                     /locus_tag="IVD64_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6149120.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(8360..9772)
                     /locus_tag="IVD64_001870"
     CDS             complement(8360..9772)
                     /locus_tag="IVD64_001870"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP6149121.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(9769..10305)
                     /gene="cysE"
                     /locus_tag="IVD64_001871"
     CDS             complement(9769..10305)
                     /gene="cysE"
                     /locus_tag="IVD64_001871"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP6149122.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(10627..12087)
                     /locus_tag="IVD64_001872"
     CDS             complement(10627..12087)
                     /locus_tag="IVD64_001872"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP6149123.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(12257..13414)
                     /locus_tag="IVD64_001873"
     CDS             complement(12257..13414)
                     /locus_tag="IVD64_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP6149124.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(13448..14818)
                     /gene="radA"
                     /locus_tag="IVD64_001874"
     CDS             complement(13448..14818)
                     /gene="radA"
                     /locus_tag="IVD64_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP6149125.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(14881..15408)
                     /locus_tag="IVD64_001875"
     CDS             complement(14881..15408)
                     /locus_tag="IVD64_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP6149126.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         4036 a         3375 c         2774 g         5233 t
ORIGIN      
        1 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
       61 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
      121 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
      181 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
      241 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
      301 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
      361 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
      421 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
      481 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
      541 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
      601 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
      661 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
      721 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
      781 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
      841 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
      901 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
      961 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
     1021 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
     1081 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
     1141 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
     1201 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
     1261 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
     1321 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
     1381 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
     1441 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
     1501 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
     1561 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
     1621 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
     1681 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
     1741 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
     1801 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
     1861 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
     1921 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
     1981 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
     2041 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
     2101 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
     2161 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
     2221 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
     2281 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
     2341 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
     2401 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
     2461 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
     2521 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
     2581 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
     2641 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
     2701 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
     2761 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
     2821 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
     2881 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
     2941 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
     3001 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
     3061 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
     3121 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
     3181 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
     3241 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
     3301 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
     3361 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
     3421 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
     3481 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
     3541 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
     3601 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
     3661 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
     3721 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
     3781 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
     3841 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
     3901 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
     3961 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
     4021 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
     4081 tttgtctgat ccggataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
     4141 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
     4201 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
     4261 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
     4321 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
     4381 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
     4441 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
     4501 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
     4561 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
     4621 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
     4681 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
     4741 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
     4801 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
     4861 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
     4921 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
     4981 aaaaaaagaa agccgatttt cagtgtcggc tttctttttt tagattctta acttgctact
     5041 tgttcctttt tcatgtcgat atgtggaata tcatcttcca aatacacatc tgagattgcc
     5101 ttgaatccaa acgactcata aaaagcttgt aggtattctt gtgcgccgat cacgattggt
     5161 ttttctggaa aatttttctg gatcatgtta atcgtttctt gaacaatctt tcttcctaaa
     5221 ccatctttac ggttgtcctg atgaacaagg acacgtccaa aatggatact ctccgcttct
     5281 tcatatactc gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa
     5341 gcttgtccat caatgtcatc aacttcttga taaggacact gttgttccac aacaaaaact
     5401 gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg acaattcttc aaatttcttt
     5461 acctgatatt ccactaagca aacgccccct gcttttttat tcttagcata ccgattcgca
     5521 cacaaaaaag caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag
     5581 ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt
     5641 caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta
     5701 aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc
     5761 tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca
     5821 cctctactat caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaag
     5881 aaatgcaata aaaataaata agaagctcat taagattttt ctaaattatt cctcattata
     5941 aaatttcaag tgttttttta ttttgagttt tactctcccg taagcgctca ttacttgctt
     6001 gtgatccatt gctaaacatt cagccatgtc tacaaaatca acgccttgca tataataatg
     6061 aaaaatttta gcttctattt ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa
     6121 taaaagatgc tgaatcgcta aaggttcttg caatgtgatc tctgctaaaa agtctggtcc
     6181 ttctgtctct attttttggt cgattgagac ttcatctttt tgatcacgac gcttcagcgc
     6241 gttctgcttc ctcaacagag aatagatttg attgcttata atgcttttat aataacttcc
     6301 aaagctaaca cctttgcttt tttcgtaagt tctcaatgcc tgatataaag cgatccttgc
     6361 ttcttgttcc caatcctcag aaacataatt tcttaaataa taggtgtttt tcaaacgtag
     6421 taaaagtgga aaatattttt gatacagggc agcaaatgct tcttcgtcac cattacgtgc
     6481 agcgcgcaat aactcctcag acataaaata attcctccat aaattcgttt atggaggaat
     6541 cttatcatca atgattcatc gaactgatta gaacgatatg ttatttattt tttctcatta
     6601 aatcgtccaa tttttgggac aattttttta actgctcttc attccatgga gaattacgtc
     6661 ggaaatcaat aaaatgcata tctttatttt cttgggctat tgttttttcc gtttttttta
     6721 tcgtcttata taattcataa gcagaagttc gtaatgctcc ttgggaaaag atcatccact
     6781 gttctgctaa atcgcttgtc gccactgtca cttgtgtcaa acggtcattc agctgaccag
     6841 cgattctctc gatataactg tcagccgttt cgtcttcttt cgtaaaaaca acatccaact
     6901 ggtatttctt ataattttgt tggatgcccg gaactagttg ggcatcgaag acaacgatga
     6961 cttccattcc ttcatatttc gcataatttg ataaacgatg taaaagagct tctcttgcat
     7021 ccgccatttt gtcctgcttt ttaagctgga caagttccgg ccacgaacca atcatgttgt
     7081 agccgtctac aatcaacagc tgttttttca tggtctactc cccctgacgt tgggaaaaag
     7141 cctgatacat aagcaaacca gcagcaacac tggcatttaa gctctggacg tgcccggtca
     7201 tcgggatcgt cagcaattca tccatttctt ttttcaatcc tggactcatt cctttgcctt
     7261 cattaccaat gactaaagca attgatcctt tggcattcca tttttggaag tttgtgccgg
     7321 tcatatctgt gccaaatacc caaaatcctg tttctttgag ctgttgcaca gcttgtacca
     7381 aattcgtcac gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc tttgttacga
     7441 taggtgtgat tccaactgct cggtgttttg gtatgatcac accatcgacg ccacttgcgt
     7501 ctgctgtccg taaaatcgac ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa
     7561 agaaggggtc ttcttttttc tcttttgttc gttcaatcag ttggtcaagg gataaatatt
     7621 cataaggtgt aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc aaagtttcta
     7681 atttttgttt aggaacccat ttgactggca cagcttgttc acgtgcgatt tctttcagtt
     7741 cttctacttt ttctcctcgt gaatcttctt gaagaaagag tttattccct cgcccttgtt
     7801 gtagcgcttc tactgcggca tggtgcccaa agacgaaatt atcttctaat gcttcttcat
     7861 tttgatcaga aactgactga tttcgtccag tttgcttttt atttgaatat ctattttgtt
     7921 ttttgtcttc aaatccacgt ttcgattgat gtttgtttgt tctttcttta cccatttttt
     7981 tcaccaactt tctgaataca ccagcggatc aattcttcca tccgctcggt ttgttttgtc
     8041 aaatgcagat agcccatgag cgcttcgaac cctgttgcga cccgatacgt cgtaacatca
     8101 gcattttttg cgctcgtatg gcttttagca tttcttcctc tgcgataata aagttcttct
     8161 tcttctgtca aaagatgttc ttccagcatt tgctgaatca aaaaggcttg tgcattcgca
     8221 gaaacataat tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc
     8281 aggtggtcac gaacatagat ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg
     8341 ttcaattgtg tgtaatctct catgcttctc ttctccatcg ggttccttgc ggtgtatctt
     8401 ctaataagat ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac
     8461 gattgcttct tgcttgattt cgttcttcga tcaattgttc aatctcttca tctaataact
     8521 cttctgctgt aaataaaata ccgaagatcg aaagccattg cgagaataaa gcttctcctt
     8581 gtgccaagac agcggcagaa actttcggct ggtctagata agtgttcagc cacttagcaa
     8641 gctcatagac cactgtgatc ccatttgctg cattaaaatc atcgtccatt tcttcgatga
     8701 atcgctgttc taaattttct aacaaagcga ttttatccgc atcatctgcc aaattctctg
     8761 aggcagtttc ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg
     8821 tggctgcttc tttcatcgtc gtttcactgt agcggatcgg acgacggtat tgtgtggtcg
     8881 ccatgaagaa acgcaagact tgtggatcaa cgtttttgat catttcatgt acagtgatga
     8941 aattccctag agatttactc attttttcat cgtcttcgcc aatcgttaca tagccattgt
     9001 gcatccagta attagcgaat tttttccctg ttttcgcttc actttgtgcg atttcatttt
     9061 catgatgagg aaattctaaa tcttgcccgc caccatggat atcgatcgtt tctcctaaat
     9121 gttttgttgc cataacggaa cattcaatat gccaaccggg acgtcctttg ccccatggtg
     9181 aatcccaaga aatctcgcca ggttttgctt gtttccataa agcgaaatct aatgggtctt
     9241 ctttttgctg ctgttcttcc cctgtacgct gacttgctcc gacttccagt tcatcgatgg
     9301 attggtcact taatttccca taattttcaa atttacgggt acggtaatac acatcacctg
     9361 aagattcata agcatagcct ttttcaacca acacttgaat aaatgctaaa atatctggca
     9421 tatgatccat gactcttgga tgcagtgtag caggctcgac attcaacgct ttcgtatctt
     9481 cttcaaatgc tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct aattcgtttg
     9541 ctgtacggat gattttatcg tctacatccg tgaaattcga tacgaaatcg actttatagc
     9601 ctcgatattc aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga gcatttccga
     9661 tatggatata attatacaca gtcggtccgc agacatacat accgacatgt cccttttgta
     9721 tagggatgaa ttcttctttt tttctggtca atgtattgta gatttttatc attctgatct
     9781 cctcactttt tctcctttga ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc
     9841 gtctggaaca tctttcaaca caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt
     9901 taccggtcct aatatctgcg cattcgcaga gagcatggct ccttgtttaa cagttgggtg
     9961 tcttttaccc gtttcttttc ctgtgccgcc taatgttacg ccatggaata aaatcacatc
    10021 gtcctcgatt tctgcggttt cgccaatgac gattcccatt ccatggtcga tgaacacgcc
    10081 agttccaatc gttgctcctg gatggatctc aacaccagtt aggaaacgcc agaattgagc
    10141 attgatcttc gccaataaaa agagccggtg ctgatataaa aaatgagaga aacgatgcca
    10201 aaacaaggca tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg
    10261 atcgtttctc tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt tccactcctt
    10321 tcgtaagaat tttatgtagg aaatcatctt cttataaaga aaagaagcgc cttttaaata
    10381 agtttcctta tcaaaagacg ctttccgcgt ggttccactt ttcttcataa tcaatgatta
    10441 tctctgacag ctaacgcctg ttatacgttc ttggctactt tgtttcctat aaaacagttt
    10501 ttcctgtttt ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa ccattcattg
    10561 ataccttgca ccacccggta tctctcttgt aatgatcagc tgcttacttt tttctgttca
    10621 tcgtgtttag ttcagtactt gatttaaatg tgctaatgat ttttcacgac caagcaattc
    10681 gatcgtttca ggcaattctg gaccatgcat ttgtcctgat acagctacac ggattggcat
    10741 gaaaagattt tttcctttta cgccagtttc tttttgaaca gctttgatgg cagctttgac
    10801 agttggagca tctactactt ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt
    10861 aggaactgtt tcaccagcaa gtacttcttt tgctgcttca ttcaatacag gatgttcatt
    10921 gaaaaatagc gtagacagat caacgatttc tgcagcatag ctcatttgag gttgatacag
    10981 gctaacgatc ttcttcaccc attcgattgt cttttcatct gggttttctg ctacacgtcc
    11041 atcagcgatt agataaggca gacacatgtc agtaaatgca tctagatcca ttgctttgat
    11101 gtaatggttg tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg atttacttag
    11161 gcgttttgga tcgaacattt tgatcaattc ttggtgttcg aagatttcgt cttcgcccac
    11221 aggtgaccag cccaataatg caataaagtt gaacattgca tctggtagat agcctaattc
    11281 gcgatattgt tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt
    11341 ttcactgttg atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc
    11401 atagatcatc agttgttttg gtgtattggc aatatggtca tctccacgta aaacgtgagt
    11461 gattttcatc aaatgatcat cgaccgcaac agcaaaatta taagttggca taccatcgcg
    11521 tttttgtatg acaaagtcgc caccgatatt atcggattcg aacgaaatag cgcctttcac
    11581 catatctgta aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa cagattctag
    11641 accttgtgct tctttttctg cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta
    11701 atgaggcatt tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt
    11761 gcaatagcat ttgtatgctc gattgctagc aagcagctga tcaatcagcg gttgatagat
    11821 ttctttacgt tcagattgac ggtaagggcc gtattctcca ggattttcag gagattcatc
    11881 ccaattcatt cccaaccaag caaggttttc caactggctt ttttcgccat cttcgatatt
    11941 acgtttttgg tcagtatcct caatacgaat aataaagtct ccatcattgt ggcgggcaaa
    12001 caaataattg aataatgcag ttcttgcatt tccaatatgc aggtgcccag ttggacttgg
    12061 tgcatagcgt acgcgtactt tcgtcatttt cttcctcttc tttcaaacta gactttgact
    12121 aacagcaaaa attccgtcag ttacggcttc tcaaatatca tttatttaaa gccactcagc
    12181 acaaatttta ccttttctaa ggtaaatagt aaagccaact ggttgaattt ccttaccagt
    12241 tggctgaatt tcttttttat tttgattgtt tagtcttttt ctctgtctct tttttattgt
    12301 cgattcctcg tcctgcatgt gcaggtttag caaagatcat gcgtccagca gctgtttgaa
    12361 gggcacttgt tacaacgact tcgatacgtt cattcatgta atgctgtcca tcttctacga
    12421 caaccatcgt gccgtcatca aggtaggcta ctccttgttg gcgttctgtc ccggctttta
    12481 ctactagaac attcatcgtt tcaccaggga tgacgactgg ttttacggca tttgccaacg
    12541 cattgatatt aagaacaggt acattttgga attcagagac tttattcaag ttatagtcat
    12601 tggttacaat gacaccatct aaaagcttag ccaatttgat caatttgctg tctacttcac
    12661 tgatatcttc aaagtctcca tcgtacattt cgacagatat accctcttct ttttgcaagg
    12721 catttaggat atctaatccg cgacgaccac gcacacgttt caaactatcc cctgagtccg
    12781 cgatgtattg tagttcgtaa agaacaaaat tagggatcat caaggttcct tctaagaatc
    12841 cagtctttgt aatatcataa atccgaccgt caatgatcac gcttgtatcc aagattttat
    12901 atttatggaa attttctcct gattttcgtt ctaacagttg actctcaact tgactagctg
    12961 tttcttgctc gatttttttg cttcgtgaac caaaaacttt cttccattct tcgattcgtg
    13021 tcgttcccat tctcaatccc agatagccta agatgatcat cagtaaaatc ggcaatacac
    13081 tattgacgaa tggaatttcc atattgtaaa gaggggtcga gatcacaaca cctaaagtca
    13141 atccgatgat cgcaccgatc gcaccgaaga gaagataagt caagctgacc tcacttagtt
    13201 tttgttctac ttgtttgata atagctgaga tatgtttggc caacagcaaa gataataaaa
    13261 agaaaataag tgcgcctaac aggctgttgg taaatgtatt attcagccat ccgtttgact
    13321 gttgaccgat tgcctgccaa gctgagggta aaaatgaaat acccaagctt gcgccgacaa
    13381 tgatcatcag caaagtaatg acacgttttt gcatcgtttt tcctccgttt cgacaatcat
    13441 ttattagtta gcggaacaca cgcttcaacg tttcagccaa agttgctact ccaactatct
    13501 cgattccttt tggcgcttcc cagttaccta agttgttttt aggtaggtat acttttgtga
    13561 agccgagttt ttggacttct ttgactcgtt gttcaatatg actgacacgt ctgatttctc
    13621 cagtcagtcc aatttcaccg atgaaacatt ccgttggctg tgttcctttt tctttatagc
    13681 ttgaagcaat actgacagcg atagctaaat caatcgctgg ttcatttaat tttaccccac
    13741 ctgcagcttt taagtaagca tcttgattct gaagaagcaa cccagctctt ttttccagca
    13801 ctgccatgat cagagatacc cgattgaaat caagacctgt tgttgttcgt ttggcatttc
    13861 cgaacatagt aggtgtgact aatgcttgga tctctaccaa aattggacgt gtcccttcca
    13921 ttgccacaac gatagcagag ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg
    13981 aaggattagc gacttcttcc aatccatgct catgcatttc aaaaatccca atctcattcg
    14041 ttgagccaaa tcggtttttt actgctcgca aaatccgaaa actatgatgt tgttcacctt
    14101 caaaatataa caccgtatcc accatatgtt ctaacattct tgggccggcg atcgagcctt
    14161 ctttggtaac atgaccgacg ataaaaatgg ctatcccatt tgttttagcg attttgagta
    14221 gttctgcagt ggtttctctt acttggctga cacttcctgc cacacttgtg atgtctggct
    14281 gcgtcatcgt ttggatggaa tcaatgataa cataatcagg ggagatatgt tcgatggcac
    14341 gactgatttc gttcatatct gtttcagcgt atagataaaa ttctgtgttg atacttccga
    14401 ggcgttccgc gcgtaatttt atctgctctg cgctttcttc gccggataca tataagactt
    14461 ttccgcctat ggctgctaat tgctgagaaa cttgtaacag caaagtggat ttcccgatac
    14521 ctggatcgcc accaatcaag accatagacc ctggcaccac acctccgcct aacacacggt
    14581 tcagttcttc taattttgtt ttgatccgag gttctttttt agggacgacg tctgcgattt
    14641 ttgttggttt tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt
    14701 gttcgatttc ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt cccaagaact
    14761 taggcgacac atacccgcaa ttttggcaaa caaattgtac ttttgatttt tttgccattg
    14821 taccgcctcc ttttatccta atcatgagac attaaatcaa gcttaagtat ctttaacgtt
    14881 ttatttactg gaagaaccaa atccgccttg acgttgagca gtcgtttgat cttgatctgc
    14941 ttttaaaaac ggcaaaaaga tcccttggcc gatacgctcg ccttttttga tcactacatc
    15001 cttgaaacca aagttggtga attggaacat gatatggcct tcatttgcct cattattgta
    15061 ataatcactg tcaatcacac ctactccgtt tgtcaacact aaaaaccgct tcaacggatt
    15121 gcttgaacgg ttagcaagct gcaaaaattc atcttctccc atgtaagctt tgattccagt
    15181 aggcacgagg gttggtttca attgtttttc attttctgat acatcttttt tcaatccatc
    15241 tgcgactgtt tccaacccct ttttccagcc tgctttccag atacttggaa tcacgacctc
    15301 ttctgctgct tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg gtagattgat
    15361 attttgatct ttatatttag aaataacttc aaatcctctt tttctcaccg gttttcct
//
LOCUS       DACBZN010000065        15183 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.065, whole genome shotgun sequence.
ACCESSION   DACBZN010000065 DACBZN010000000
VERSION     DACBZN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.065"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            231..740
                     /locus_tag="IVD64_001876"
     CDS             231..740
                     /locus_tag="IVD64_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6149127.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            833..1995
                     /locus_tag="IVD64_001877"
     CDS             join(833..1109,1109..1995)
                     /locus_tag="IVD64_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6149128.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2275..3255
                     /locus_tag="IVD64_001878"
     CDS             2275..3255
                     /locus_tag="IVD64_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6149129.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3377..4795
                     /locus_tag="IVD64_001879"
     CDS             3377..4795
                     /locus_tag="IVD64_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP6149130.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4866..6335
                     /locus_tag="IVD64_001880"
     CDS             4866..6335
                     /locus_tag="IVD64_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6149131.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6345..6812
                     /locus_tag="IVD64_001881"
     CDS             6345..6812
                     /locus_tag="IVD64_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6149132.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6848..7720
                     /locus_tag="IVD64_001882"
     CDS             6848..7720
                     /locus_tag="IVD64_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6149133.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7801..10335
                     /locus_tag="IVD64_001883"
                     /pseudo
     CDS             7801..10335
                     /locus_tag="IVD64_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            10491..11264
                     /locus_tag="IVD64_001884"
     CDS             10491..11264
                     /locus_tag="IVD64_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6149134.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11257..12048
                     /locus_tag="IVD64_001885"
     CDS             11257..12048
                     /locus_tag="IVD64_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6149135.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12058..12549
                     /locus_tag="IVD64_001886"
     CDS             12058..12549
                     /locus_tag="IVD64_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6149136.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12567..12977
                     /locus_tag="IVD64_001887"
     CDS             12567..12977
                     /locus_tag="IVD64_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6149137.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12981..14381
                     /locus_tag="IVD64_001888"
     CDS             12981..14381
                     /locus_tag="IVD64_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP6149138.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14404..14778
                     /locus_tag="IVD64_001889"
     CDS             14404..14778
                     /locus_tag="IVD64_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6149139.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYDSLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5269 a         2128 c         3040 g         4746 t
ORIGIN      
        1 agaaatgaat tataggttta gacctttatc atttctcgtt ttattttatt gtatcaaggc
       61 tctatcataa gtggtaaaat ctacttttaa atcacatgta atcattgata agtccagtgt
      121 tttagcggat aatcagcttt ttatttattt tttcttataa atttgataaa tagaaattag
      181 tatagcccta aacaaattat ttgtgtttta ttgtttaggg ctatactata ttggtacaag
      241 gaatagaaag aggtgtttat gtggttgata aaattcagtt taatcaagtt atagaagaat
      301 tcatcaaagt aaataattta atgacccata ttcaaaaacg accaatcaga ataactaatg
      361 atctaaaact cagtacgagt atgattcatt tgattgatgt gattggaaat tatcctgaaa
      421 ctagtattac ggaattagct gatcgattag gcgttaccaa aggagctgta tcccaacaga
      481 ttcccacttt agagaaaatg aatcttataa gcatttctca aaaaaaagga aataagaaaa
      541 ctaaacttgt atcgttaaca aaatatggtc gaaaggtgta cgaatcacat aattatttac
      601 atgaagagct ttatagctca ataaatgaag cgttagaatc attctcacca gaccaaattt
      661 caaccattaa acagattcta gaaaaagtat cttatacaat caatgaatat caagtaagtc
      721 tgacaagaaa ggaagattga catgaaaaaa atgatttttg aatcggctac actagactag
      781 acagaaaaaa taaggtgtgt aaaataaatg aaaacacact ggaggaattt ttatgtcaag
      841 acgtcaacgt agaacctatt caaaagaatt caaacaacaa gtcgtcgatc tttatctcgc
      901 aggaaagccc cgagctgaaa tcattcgaga ttatgagctg actacctcgg cctttgataa
      961 gtgggtcaag caagcgcaaa ctactggctc gtttcaggaa aaagataact tgatccctga
     1021 acaagtagaa ctaattgcat tacgaaagaa aaataaacag cttgaaatgg agaatgacat
     1081 tttaaagcaa gcggcgctga tattgggacg aaaagacaag taattgatac caataagcac
     1141 aaatactcaa tatcagcgat gtgtaaagtg ttaaacatct cttgccaaac ctattattat
     1201 caagccaaac cagttgaaga tgaatcagaa ctggaagaac tcgttcaaga agaatttatt
     1261 cgcaatcgaa aagcttatgg taccaggaag ctgaagcagt gcttggccaa tctaggtctt
     1321 caagtgagtc aacgcagaat cggccgtatg atgaaacagc gtggcttgcg ttcaacatac
     1381 acattggctc atttcaaatt ccattcttct acttgtaacg aagccaagac cgccaatatt
     1441 ctagacggaa ccttcctaca ggaaaagtca ttagaagcga ttgtaaccga ccttacctat
     1501 gttcgtgttg ggaagaaatg gcagtatatc tgcctgattc ttgatttgtt taatcgagaa
     1561 atcatcggct attcatgcgg ggagaagaaa gatgcgacgt tagtcaagga agcttttgca
     1621 aagattccgt actcgttgaa taatgtacaa ctttttcaca cggatcgtgg aaaagaattc
     1681 gataaccaga cgattgacga aattttatgg gcctttaata tcacgcgttc cttaagcaaa
     1741 aaaggctgcc cctatgataa cgccgttgca gaatccacct ataaatctgt caaagtagaa
     1801 ttcgttcatc aataccgctt tgagacattg gctaatttgc gattagagct atttgattac
     1861 gtccattggt ggaataccat ccggctgcac ggcacgttag gctatgagac accgatcagt
     1921 tttcgacagc agagattggc gcagcgaatc cttgataatg agctcggatg tgataccggt
     1981 ggcgaggcac cttaattatt tgaatgagct tctgccttag agaatcagat tcgaggaggc
     2041 tcgttgtcaa gggcagtcgg aacaccagca gcaagcttac gcaatacctt ataatttttg
     2101 tcaaaaaaag tgttgccatt ccacatatat ctttaaacag ctattgccag ataaaaataa
     2161 atgatattat atatataaat acttatccga aaaggggaat gagagagtca aggttcttgg
     2221 aatgtcgaat agtcgagtag gtagattttc tatctacaac cttgttgttt aaaaatgaaa
     2281 aaaattggaa ttagagaagt tgcgaaagag gcaaatgttt ctcctacgac tgtgtcgagg
     2341 gtattgaatg atcgtggata tttgagtgag gaaacaaaaa gaaaagtttt tgatgccatg
     2401 aaaaaattag aatactatcc taatgaactg gcgagagcac tttttaaaaa cgaaacatat
     2461 accgtaggtt tgatttttcc tacaattact aatccatttc acgctgagtt aattcaagat
     2521 atcgagttcg agctttccag caggggatat aaagtgttgc tgtgcaatag cttaaacaat
     2581 ccaacaaaag aaaaagagta cttgacgatg ttgagaaaga atcaggttga tggaattata
     2641 gtaggaacgc acaataaaga tattgcggat tatgatattc cagggctacc tattgtagcg
     2701 attgatagaa gtcttggtga aaatacagtt acagtatctt gtgataatta caggggtggg
     2761 gaattagcta ctcaactact tgtaaatcat ggctgtcaga atatactgtg tattcgggga
     2821 gatagtaaga taaagctacc cgcgaatagt aggactttag catataaaga tgttttgaat
     2881 aaattcatgc tggaaccgtt agtcctagaa gtaccatttg taaaagaaat caatgaaaaa
     2941 aatgaaataa ttaagacata tctggaaact catccaaata ttgacggggt ttttgcagga
     3001 gatgatttat tggcttcttt ggcgattaac aacttaggaa gttcgggcaa aagagttcca
     3061 aaagaggtta aagtgattgg atttgatgga gctcaacaaa cattgattta taatccagaa
     3121 ttatcaacta ttcaacagcc tatagatcaa atatctaaag tagcagtaaa aaaattattg
     3181 aatatgatta agggcgaaaa ggagacggat gaactttcac ttccagtaaa gctcataaaa
     3241 aaaaatacag cataagttaa atcggcgaaa aattccttcg tcgatttaaa aatattttta
     3301 tgaaacgggt tgacatatga aacgggttga catatattag ttgtgaagat aaaaaccttt
     3361 tcactggagg aagaaaatgg agaaattatt aattaaaagc aataatcatc aactttacta
     3421 ccaacctaag gatgtttggg tcggagatgt tatgccttac gggaaagaag gaaaattctt
     3481 tatttatcat caacgtgata ctagaaatcc agttcctttt ggagaaccgt ttggctgggc
     3541 tttagcaact acgaaagatt ttgttcattt tgaagacttt ggggaatctc ttttaagagg
     3601 ggatgacaat gagagagacc agtttatcta cgctggttct gtatttgagg caaatggaaa
     3661 ggttcacgca ttttatacag ggtataatcg tgaattctta gcgcagggaa agacatctca
     3721 agtgttgctt catgctgaga gtgatgattt tgttcattgg gaaaaatcag aaacagcatt
     3781 ggagttagaa ccacaagaag ggtatgatat tcgaaactgg agagatccat ttgtcgtttg
     3841 ggatgatgaa aaagaagaat atttgctaat tttaggggcg cgaaagggtc aggataaaca
     3901 tctacaaacc ggacgtttag tggaatttac atcaaaagat ttaactaatt gggaattcaa
     3961 aggtgatttt tgggctccgg gattgtatac catgtttgaa atgccagaac ttttcaagat
     4021 tggtgactgg tggtatcttg tctattcaga atactcagat ggaaataaaa tactctatcg
     4081 aatgagtcaa agtttggaag gtccttggat taagcctaaa gatgaagctt ttgatggacg
     4141 ggcttattat gcaggtagaa cagcttatga tggaaaacgt cgagtcctat ttggatgggt
     4201 accaacaaaa gatcaagaaa atgataaaaa caattattta tggggaggta ctttggttcc
     4261 tcatgaagtc tatcaaaaag aggatttttc acttggggta aaacccatag acgaagtgtg
     4321 gaatgcattc ataaatttac atgatattgc cgatgtagag atagaaacag cagacacaag
     4381 agaagaagta acgcttgtca aaaatagtgg aactttgttt aatttagaaa caacaatttc
     4441 atttgatgag aatcttagtg acttttcact gagattatat aaaaaccttg agacagatga
     4501 atcttacgaa tatcgcttca atttagatga ggggcgtctt acatttgata aaaacccttg
     4561 ttacccttgg tttcaatata tggataaagg acttattaga cctatatctt taaagcctaa
     4621 ccaatcttat aaactaaaag tgattgttga tgacaatata atgacacttt atattgatgg
     4681 aatcgctctt aacgctcgtg tacatggttg ttttggtcaa gcaattacta ttacagcaac
     4741 aaatggatca atgactgcaa taaataccaa gttttcaaat gaaataaaga aataataaac
     4801 gtcaatcatg tggcaggtct gaaaatcaaa catgacctgt cacatttaaa agggaggata
     4861 aatgaatgga ctacaaaaaa attgccgaag atgtattgga aaacgttgga ggaaaagaga
     4921 atatctcaag cgctacacac tgcattactc gtttacgttt aattcttaaa gacaaaagca
     4981 aagcgaatat ggaaatgctt gaggaaatag aaggtgcaaa aggtgttctt ttcaatagtg
     5041 gacagttaca agtaattttc ggaacaggta taattgaaaa ggtttacaaa gagttcgttg
     5101 aattgacagg agctaaggaa gtatccgttt ctgaaatgaa agacgaagga gtcaataatt
     5161 caggaaatag acttcaacga gcttttaaag ttttctcgga tatctttatt ccgatcattc
     5221 cagcttttat tggggcagca atggttttag gaattcgttc gttgattacc actcctggaa
     5281 tgtttggaat ggatggctca ttcgctgatc acaatgtatt cctaaatgat ttatccaaat
     5341 ttctgggtat tattgcaacc acatttgatt atttaccagt tttagttatg tattctgcaa
     5401 caaaacgttt tggcggaaac cctattttag gattacttgt aggatttgtt atgattcatc
     5461 caggactagc caatcggaat gatttcgtca tgggaactat aaagcctgat tattggcatt
     5521 tatttggatt aagcattcca gcagttgcgt ttcaaggggg tgtttttcca gcaattttga
     5581 cttcttggtt tttagcaaaa gttgagaaaa ttacaaataa atattctcct caagtacttg
     5641 cttacatttt agtcccaact gtgacaattg ttcttgccaa ctttgcacta tttttagtgt
     5701 ttggtccatt ggggaatttt atcggtaatg ttttaggtgg aatcattgat ttcctatata
     5761 tgaagatggg tgcctttggt gcttttgttt ttgcaggcgc gttacaacca ttagttgtaa
     5821 caggaacaca acatgctatt caaggtattg aggcaaactt agtcgcaaca actggattca
     5881 attatatcca acctatttgg tcagtttcaa ttattgccca aggtggcggt tgtattggta
     5941 tgtatttact ctttaagaaa aaatctaaag atagagatat tgcgatttca agctttattc
     6001 caacactggt aggtattact gaaccggcta tctttgctgt taatttgaaa tattcaatcg
     6061 ttccttttgt ttgctcgttt attggtgctg gttttggtgg agcatttatg aaaatttttg
     6121 atgtaaaagc cattggacaa ggattgactg tattgccagg tctgactatt gctaatccag
     6181 ttcttcctta tatatttggt aatctcatag cattcatttt accaatcatt tttattctcg
     6241 cttatgataa atataaagtg tttatccctg aagctaaaca ccgtaaattt gcagaaaaaa
     6301 ctgactcaaa aaaattgcca gttcaagtaa agtgagatga gagcatgtta aattttttta
     6361 gaaaaaatga aataagcgtt gtagcacctg caaccggtgt ttttgaaaaa ctggagaaat
     6421 taagtgatcc agtcttttca aaaggaatga tgggacaagg atttgcaatt gatgcaaaaa
     6481 acgaaacgat tgtatctcca atcgatggtt ttgtttcaag tatttttcct acaaaacatg
     6541 ccttaggaat aaaaacaaaa tcaggaattg agatacttgt tcatgtagga atagatacag
     6601 ttgagctaaa tggtgaggga tttgatatta aagttcaaga aggacaaaaa gttaaggctg
     6661 gggatattct tatgaccatt aattttgtag tttttgaaaa gaatcacaag actaaagatg
     6721 tcattgttgt ttttccagac taccaagacg aaattttcat tccgaaactt gaagaagtaa
     6781 aacaaggaca agaattgttt gttcttggct aatgataagt aattaataat agaatcgagg
     6841 aaaatgtatg gtttttgggg ctattgaagc tggtggaaca aaatttgttt gtgccattgg
     6901 agataaagat ttgaacatat ttgaaaaagt cagctttccc acgactacac ccaaagaaac
     6961 aatgaattta gttttagatt tttttaatca tcataaagat gatttagaag gtatcggagt
     7021 gggatcgttc ggtcctattg atattcattg tgaatctaaa acgtatggct tcattacatc
     7081 aacgccaaaa cttgcttggc aggattttga ttttattgga agtttaaaaa agcaacttaa
     7141 tattccaact gcttggacga ctgatgtaaa tgcctcttgc tatggtgaat atgtttctgg
     7201 aaatggcaga ggcctttcaa gtgttgttta ctataccgta ggaacgggca taggaggtgg
     7261 cgctatccaa aacggaaact tcattgaagg ttttagtcat ccagaaatgg gacatatgct
     7321 tgtaaaacga cacaataaag acaacttttc tgggacatgt ccattccacg gagactgttt
     7381 agagggctta gctgctggtc cggcaatcga aagaagatca gggaaaaagg gacaagaagt
     7441 gactgaaaat gacgttactt gggaaataga agcttattac attgctcagt gtgcatataa
     7501 tacaacattg atgttatcac ctgatgtcat tatatttggt ggcggagtaa tgaaacaaaa
     7561 acacttaaaa aagaaagtac aagataagtt tattgagttg atcaacggat atgtcgctat
     7621 tcccgatgtt gacaattata ttatgacacc aaaattgtta gataattctg gtattgtagg
     7681 ttgtttagct ttagcaagag acgttaaaga aaaatcttaa tttggaaaaa agatcgtctc
     7741 gactatttgt ttggttgatg cgatcttttt ttcaattagt aattgaaggg acgcctaaaa
     7801 gtgaaaaaaa aaatgaaaga tgaagtatta gagtatatca aaaataattt aaagtattac
     7861 gatttttctg ctcaagatat tgcaatgaaa ttttgtatta aaagaaatgt cgcttcccac
     7921 tatttgaatc agttattttc agatggaaaa ttactaaaga atgattctgt tagaccagta
     7981 atgttcaagt ataatcaaca aaagccaaaa gactgctttt ctaagtttat tggagcagac
     8041 atatctttga aatccactat agataaatgc aaagctactg taatgtatcc ccctaatggc
     8101 ctcccattaa taataaaagg aaacagtggc gttggaaaaa gttttttagc aagtttgatc
     8161 tatcagtatg ctcttgatag aaaagtaatt cataatgacg caaaatttgt tgttgtgaat
     8221 tgtgcagatt atgcaaataa tccagagcta ttgtcagcag ttttatttgg atataaaaaa
     8281 ggagccttta ctggggctga aaaagatact gcagggatgc tatcttctgc taatggagga
     8341 tacctttttt tagatgaggt tcacaatcta agtgcagaaa accaagagaa attgtttctt
     8401 cttatggatt ctcaaaagta tcgtaaactt ggtgaaagtg tgcaatggga gtatgcaaat
     8461 gttcgcctta tcttagccac aacagaagat aaaaatacct ctttgttagc aacttttaga
     8521 agacgcatcc cagctgaaat cactctgcca gattacaata atagaagtac atctgaaaaa
     8581 attcaattat tatttgaatt tcttaaagat gaagcaatta cgattgattc aaaaatattt
     8641 tgttcgattg aactttttac tgatctttta tcgaaaacat ttgaaggaaa tgttggagaa
     8701 ttgaggaatg aaatcaagct tctttgtgca gaaggttatt taggaaataa gagaaagtct
     8761 tcaatttatt taggggacaa actagacagc ggtttttgga tagatccaga aattagtata
     8821 gatttaaaaa aatgtttctt aaatcacttt ctgaaattga tttgattgaa ctttttcaaa
     8881 atcacatttc aattgaagaa agcatgagtc aattaagaga tagaatactt aaagagtgtg
     8941 caggttcttc tgagtataat tatttagaaa gtgacgaatt tatagcatta agaaactatg
     9001 ttgttaataa aatatcacca attattgatt cgacaggtct atgtttatta gatgaattct
     9061 tggcggatat cagtctattt ataatgttta ttgatgtaat tgaagaaaca tcaagaaatt
     9121 ttttgctgtc taagttcaga aaatttcgta tgaaaaatga taaacaaaaa ttattggcaa
     9181 atgagatttg gagtagttta gaagtcgaag aaagtaaaca agaacttttg ttgaaatgga
     9241 tactttttct tgtaaagaag ttctatgtga aaataccaga aacccattgt ctgatagtta
     9301 tgcacggtaa aataacagct tctgcggatc gcaagggaaa caaataagtt gctgaataca
     9361 tatgtttatg aggctttcga tatgccaatt gaaggtgaga ctagtgactt aattaaccta
     9421 atcaacaatt tttgtaaaag tatagatacg acgaatggac tcattctgct agttgatatg
     9481 ggatcattgg aacaaatgta tgaaaaaatt gaatccaatg taataggtga tttggtaatt
     9541 ttaaacaatg tttctacagc tttagcgatt gaatgtggga ttcaagtttg ccaaaaaaag
     9601 ccaattagtc atttttacca gatggatttt gattcgtttc aagttaaagt tcagtattat
     9661 aaaggattat cacaaaagaa aaatgtaata gtatcttgtc tctctggtga ggggatttct
     9721 gaaaaagtta aggatatttt aaaacggtat ctcgataatc aagtggagat tttgattttt
     9781 gattttaacg cattaaagaa aatagcaaag gaaaaagata ctatgatttt taagaataca
     9841 atttgtgttc tttcaaccac tgaattttac attcaaggaa tcgattgttt aaatttagaa
     9901 aatctaatta atgggaatca aaccttagaa aaattaaata agtatatgga ctcggatagc
     9961 tgtgagttct gcttaaatga attagttaaa ctctttacta ttgaaggcgc tagtactaag
    10021 cttcgctttt tagattccaa aaaagtattc aatgagatag aaaaagtact atatttgtat
    10081 gaaaaatatt acgatgtcaa aatcccaagt tttctgagaa ttaatctatt tctacatctt
    10141 tctggaatga ttgaacgaat tatgattggt gatggaataa caaatgaaca tttgaaagag
    10201 ggagcgcaac tatttgatac ttttttaaat gtatcaaaag attttttttc agaaatcaaa
    10261 gatatttact gtattgaaat acccaaagga gagtatgagt taatctatac aatttttgag
    10321 cagactattt tctaaatagc atactagcgt ctaaaaaaat gaaagggtgt ccaaagtata
    10381 tttagacgct catttaatta atatttactg tattttcccg ctttattgga taatgttagt
    10441 tggcacagta attgcttata taaaagtata agaagaaagg agagaacaaa atggcgtttt
    10501 ggcaaacatt tttagttgtg ttgtatggat ttttcattaa ctgggataag gaagggccca
    10561 aaatcggaac ctcccaacct gtcgttgcag gtttcttgac cggacttatt atgggagata
    10621 ttcaaactgg actttatatc ggtgggactt tgcaattaat gacactaggt attactagtt
    10681 ttggtggtgc atcagtaccg gattatcaaa cagcagcatt gatcggtact tatttagcaa
    10741 tctcaactaa ccaggatgct tctataggaa taactttagc aataccggta gcaatgttaa
    10801 ttgtacaact agatgttcta aagtggtcaa caaacatttt ttttcaacaa aaggcagaaa
    10861 aattcgcaca aaaagaagat tatcaaaaat tagagtggat gcaatatttg gcatcaatta
    10921 atacaagctt tgtatctgga attcctgttt tgttaactgt gatattaggc ccaacgttag
    10981 tgggaagtgc aatcaattat attcctgaat gggttaccgg tggtttaaag gtagcagggg
    11041 gactattacc tgctgttggg ataggaatgc tacttaaata tttacctaca aaagattact
    11101 ttgcctattt gttagttggt ttcgttttgg cagtttattt ccaaatacca attttaggga
    11161 tttcgttact tggcgctgca atcgcactaa tcgtatataa aaatagatca aataatactc
    11221 aagtagcacc agctgcaaca ggaggttttg acgaagatga ataagaaact gacaaaaatc
    11281 gacaaacaag taattttccg gtggattctc aatggtgcaa cttcattaaa ttatgaaaaa
    11341 atggaagggt tgggttatgc gtattctatg cttcctttta tcaaggacac atataaagat
    11401 gatcctgaag gaatgaaaca atccgtttta gctcacttac aattttttaa cactacgccg
    11461 tatacagcgc cttacattat tggtatgaat gttggaattg aaaaagaaga aaaaaataag
    11521 tctttggaag cagtaagtgc tctgaaaaca ggtctaatgg gtcctttagc gggaattgga
    11581 gatagtgtat ttgtagttat tccatggact atttttggag caattgcagc taatatggca
    11641 ttggaaggta gttttgttgg aattattttg tggatactgg catcaatttt attaaggatt
    11701 actgcttatc ctttatacct tgctgggaaa gaatcgggtg cgagcctact aactcaaatt
    11761 gaaacaaaat taaagtctat tactgatgca gtatcaattt tagggttaat ggtggtcggt
    11821 gcactcattg ccacagttgt aaaagtgaat gttggctttg aatttaaaca aggcgactta
    11881 acaatgaccg gaaatgaaat ctttgatcaa gttatgcctg gtttattacc tgcatgtgta
    11941 gtagcaattg tgtattattt gttaggacga aaggttaaac cagtttatgt aattctatta
    12001 atcttagtag tttctattgc gttaagagcg ttaaatattt tgaagtaggt gttgaatatg
    12061 gatgaaaaaa tgaaaggtat tatacatgtt cgtattgatg atcgaatgat tcatggacaa
    12121 gtagcaacac aatggacaaa tcaattgagt gctaacagaa ttatggttat taataatgaa
    12181 gtagccaatg atgacgtaaa aaaagctgta gttcgcctcg ctgcaccacc taatgttaga
    12241 acttcaataa ttacaaggga agtagcagtt aaaaatattc tttctggtaa atatgaagga
    12301 caaaaagttc ttattgttgc agtttctcca atcgatgtta aatttttgat tgataacggt
    12361 ttaccaataa caagtgtaaa cgtaggaaat ttatcaagaa ggaatggaac agaacgcatc
    12421 cgtccgacga taaatattac tgaagaagag agagaggctt ttaaagaact aattagtgat
    12481 ggagttgaag tgacagtgat acaaactcct agagattcaa aagaattttt aaaggatttt
    12541 atttattaag aacagaggat gagtggatgg agtttataat tacatctcat ggaaatattg
    12601 ccattggatt tgtagataca ttaaaaatgt tttttggcga taatttaaat tgtcatgtgg
    12661 taacacttgg agatgcaggc attgaagaat tccgtgaaaa cattgaaaaa atagtttctc
    12721 aagtaaaaaa tcaagaagtt atggtatttg ctgacttgat tggtggaaca cctttcaacg
    12781 aatttgctaa aagaagtgaa gtatttcaaa atgggtttca acttcttggt ggcatgaatt
    12841 taggaatttt gattgaggct gtcaatttga gattacaagg aaaaaaaagc gatgatatca
    12901 tcgaaaatct aagaaatatg aatacaattg cgtgtcttgc agatatgcaa agacatacta
    12961 acgaagagga tgagtgataa atgcaaaaaa ttttttacaa gcctaaagat ttttggtttg
    13021 gggattgcat gcctttttat aaagatggaa ctttttatct atttcatcaa cgagatacga
    13081 gaaatcctaa acctttaact gatccttttg ggtggtcact agttacaacc actgattttg
    13141 tgaaatttga agaatacggg gaagtaattc atcgtggtgg caacgatgac attgatcaat
    13201 acatttacgc tggaagtatt gtccaaggaa tcaatgagta tttagctttg tatactggac
    13261 ataatcgcaa agctaagttg gatggaaaaa cttcagaagt gttaatgatt gcaagttcta
    13321 aagatttaat tcattggaaa aaggatggcg aagcagcaaa acttgttcct caagaaggat
    13381 atgataagaa tgattggcgc gatccttttg caatttttga tcaagatcat ggaaaatacg
    13441 ttttgatact aggagctaga aaaccagcaa gtaagaaaaa gccgacgggg agattggtct
    13501 attttgaatc cgaagattta aaagattgga atttcaaagg agactactgg gctaataatg
    13561 aatttaacat gattgaaatg cctgatatat ttgaaattga tggaacgtgg tatttacttt
    13621 tttcagagta ttgtgaagat aaaaagacta aatatcgagt cggagcagga ttattttcag
    13681 attggaaatc accagtagac gaagctttcg acggaaaagc ctattatgca gcaagaacag
    13741 tcggaactaa tgaaactggc cgttttctaa ctggctgggt ggcaacaaaa gaaggggaga
    13801 acgatcaaaa taactgggat tggggcggtg cacttgttcc acatcaaatt attcaaaaga
    13861 cagacaaaac tcttggtgtt aaactgcctg aaaaaataat tgagaatttt aaagaaatca
    13921 ctccattttc aagttttaag ttagagaaag aatttggaaa aaaagaaaga gtaattgttc
    13981 ctaaaatcga agattatttt ctatttgaaa caactttgac tattacatcg ttaacaaagg
    14041 agtttgcttt aaaattattt gtagatagtg atactcaaga aggatacgaa tttcgtattt
    14101 cattgattaa taatcagctt actttcggaa aaactccgaa ctatccttgg cctcaaatgt
    14161 ttgataaggg gctgcaacga ccactatttt tggaaatagg caaagaatat gaactaaagt
    14221 taattgttga tgatacgata gctgttttat atattgataa tgttgcttta agtactagaa
    14281 tgtatgataa agtcggaaca gcattaagta tgactgttac ggagggagca atcaaggtag
    14341 ataaactaga ctattcaata aattatgaat gtggatttta aaacaattga agggatgatg
    14401 taagtgaaat taaatggttt acaacatatt ggaattccaa caaaagattt tggagcttca
    14461 aaaatctttt atgattcctt agggtttagt ctgattaatg aagaaatgaa tgtgggaagt
    14521 aaagtcggct ttttcgagtt gaatggaacg ataatagaaa tatgggaaga taaaactact
    14581 gagtgtagag gagccattga tcatctagca ttagatacgg atgacataga tgaggtattc
    14641 aattcaattc aagatttagg atatgaatta ttagataaaa atattatgga attaccgttc
    14701 tgggaaaatg gaatccgtta ctttaatttc tacggaccaa atcatgagat tatagagata
    14761 agccaaatga agaaataaat agataacgaa gtgctctaag ttggtatgaa cgaaaagtac
    14821 ttcatttttt ggtatcagca tttcaatagt ttaatgagaa tttactattt caaattaatt
    14881 caaagtgaca gtatttttta cttatttggt ttcaaatttt gtaatccttc ctatttattg
    14941 gaatacactc cacattttgt atacaagtca tatcatgttt tagcaaagct cttaccaagc
    15001 aaagttataa tgatgttttc caaagtttga tctttcgatg gaggctaaca catatttttt
    15061 gcaggacagc caaaattaga tgtatagaat agagtctgtg aggcgtatag acagtttatt
    15121 gtctgtatgc ctttttttat tttgaaaaat ttttagtatt tttcgaaaaa tgctcactcc
    15181 tag
//
LOCUS       DACBZN010000066        14631 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.066, whole genome shotgun sequence.
ACCESSION   DACBZN010000066 DACBZN010000000
VERSION     DACBZN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.066"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="IVD64_001890"
     CDS             complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="IVD64_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6149140.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1819..3042)
                     /locus_tag="IVD64_001891"
     CDS             complement(1819..3042)
                     /locus_tag="IVD64_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP6149141.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3073..3912)
                     /locus_tag="IVD64_001892"
     CDS             complement(3073..3912)
                     /locus_tag="IVD64_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP6149142.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3929..4609)
                     /locus_tag="IVD64_001893"
     CDS             complement(3929..4609)
                     /locus_tag="IVD64_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6149143.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4611..5684)
                     /locus_tag="IVD64_001894"
     CDS             complement(4611..5684)
                     /locus_tag="IVD64_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6149144.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6331..7002)
                     /locus_tag="IVD64_001895"
     CDS             complement(6331..7002)
                     /locus_tag="IVD64_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6149145.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="IVD64_001896"
     CDS             complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="IVD64_001896"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP6149146.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10426..10863
                     /gene="ndk"
                     /locus_tag="IVD64_001897"
     CDS             10426..10863
                     /gene="ndk"
                     /locus_tag="IVD64_001897"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP6149147.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10885..11751)
                     /locus_tag="IVD64_001898"
     CDS             complement(10885..11751)
                     /locus_tag="IVD64_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6149148.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(11911..14004)
                     /locus_tag="IVD64_001899"
     CDS             complement(11911..14004)
                     /locus_tag="IVD64_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6149149.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4246 a         3137 c         2497 g         4751 t
ORIGIN      
        1 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
       61 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
      121 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
      181 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
      241 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
      301 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
      361 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
      421 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
      481 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
      541 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
      601 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
      661 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
      721 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
      781 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
      841 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
      901 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
      961 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
     1021 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
     1081 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
     1141 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
     1201 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
     1261 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
     1321 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
     1381 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
     1441 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
     1501 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
     1561 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
     1621 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
     1681 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
     1741 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
     1801 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
     1861 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
     1921 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
     1981 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
     2041 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
     2101 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
     2161 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
     2221 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
     2281 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
     2341 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
     2401 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
     2461 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
     2521 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
     2581 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
     2641 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
     2701 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
     2761 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
     2821 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
     2881 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
     2941 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
     3001 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
     3061 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
     3121 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
     3181 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
     3241 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
     3301 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
     3361 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
     3421 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
     3481 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
     3541 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
     3601 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
     3661 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
     3721 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
     3781 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
     3841 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
     3901 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
     3961 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
     4021 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
     4081 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
     4141 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
     4201 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
     4261 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
     4321 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
     4381 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
     4441 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
     4501 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
     4561 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
     4621 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
     4681 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
     4741 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
     4801 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
     4861 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
     4921 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
     4981 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
     5041 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
     5101 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
     5161 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
     5221 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
     5281 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
     5341 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
     5401 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
     5461 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
     5521 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
     5581 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
     5641 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
     5701 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
     5761 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
     5821 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
     5881 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
     5941 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
     6001 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
     6061 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
     6121 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
     6181 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
     6241 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
     6301 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
     6361 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
     6421 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
     6481 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
     6541 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
     6601 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
     6661 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
     6721 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
     6781 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
     6841 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
     6901 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
     6961 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
     7021 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
     7081 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
     7141 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
     7201 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
     7261 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
     7321 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
     7381 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
     7441 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
     7501 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
     7561 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
     7621 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
     7681 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
     7741 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
     7801 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
     7861 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
     7921 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
     7981 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
     8041 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
     8101 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
     8161 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
     8221 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
     8281 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
     8341 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
     8401 ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca
     8461 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
     8521 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
     8581 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
     8641 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
     8701 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
     8761 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
     8821 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
     8881 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
     8941 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
     9001 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
     9061 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
     9121 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
     9181 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
     9241 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
     9301 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
     9361 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
     9421 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
     9481 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
     9541 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
     9601 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
     9661 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
     9721 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
     9781 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
     9841 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
     9901 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
     9961 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    10021 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    10081 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    10141 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    10201 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    10261 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    10321 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    10381 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    10441 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    10501 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    10561 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    10621 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    10681 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    10741 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    10801 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    10861 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    10921 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    10981 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    11041 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    11101 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    11161 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    11221 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    11281 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    11341 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    11401 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    11461 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    11521 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    11581 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    11641 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    11701 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    11761 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    11821 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt
    11881 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    11941 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    12001 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    12061 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    12121 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    12181 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    12241 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    12301 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    12361 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    12421 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    12481 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    12541 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    12601 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    12661 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    12721 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    12781 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    12841 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    12901 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    12961 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    13021 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    13081 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    13141 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    13201 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    13261 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    13321 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    13381 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    13441 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    13501 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    13561 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    13621 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    13681 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    13741 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    13801 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    13861 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    13921 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    13981 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    14041 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    14101 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    14161 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    14221 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    14281 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    14341 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    14401 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    14461 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    14521 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    14581 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat a
//
LOCUS       DACBZN010000067        14237 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.067, whole genome shotgun sequence.
ACCESSION   DACBZN010000067 DACBZN010000000
VERSION     DACBZN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.067"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            168..362
                     /locus_tag="IVD64_001900"
     CDS             168..362
                     /locus_tag="IVD64_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149150.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..929
                     /locus_tag="IVD64_001901"
                     /pseudo
     CDS             631..929
                     /locus_tag="IVD64_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1026..1460)
                     /locus_tag="IVD64_001902"
     CDS             complement(1026..1460)
                     /locus_tag="IVD64_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6149151.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="IVD64_001903"
     CDS             complement(1692..2402)
                     /locus_tag="IVD64_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6149152.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="IVD64_001904"
     CDS             complement(2392..3252)
                     /locus_tag="IVD64_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP6149153.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="IVD64_001905"
     CDS             complement(3257..3895)
                     /locus_tag="IVD64_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP6149154.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="IVD64_001906"
     CDS             complement(3910..4209)
                     /locus_tag="IVD64_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6149155.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="IVD64_001907"
     CDS             complement(4252..5736)
                     /locus_tag="IVD64_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6149156.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="IVD64_001908"
     CDS             complement(5756..6217)
                     /locus_tag="IVD64_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6149157.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IVD64_001909"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IVD64_001909"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP6149158.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="IVD64_001910"
     CDS             7520..8290
                     /locus_tag="IVD64_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6149159.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="IVD64_001911"
     CDS             complement(8563..9390)
                     /locus_tag="IVD64_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6149160.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="IVD64_001912"
     CDS             9958..11193
                     /locus_tag="IVD64_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6149161.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="IVD64_001913"
     CDS             complement(12192..12452)
                     /locus_tag="IVD64_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6149162.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="IVD64_001914"
     CDS             complement(12479..13780)
                     /locus_tag="IVD64_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6149163.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..>14237)
                     /locus_tag="IVD64_001915"
     CDS             complement(14072..>14237)
                     /locus_tag="IVD64_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6149164.1"
                     /translation="QEVKEGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQE
                     VKIKDSCFEF"
BASE COUNT         4602 a         2899 c         2210 g         4526 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctgg
//
LOCUS       DACBZN010000068        13917 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.068, whole genome shotgun sequence.
ACCESSION   DACBZN010000068 DACBZN010000000
VERSION     DACBZN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.068"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            131..505
                     /locus_tag="IVD64_001916"
     CDS             131..505
                     /locus_tag="IVD64_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6149165.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVD64_001917"
     CDS             complement(619..1248)
                     /locus_tag="IVD64_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6149166.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVD64_001918"
     tRNA            complement(1382..1454)
                     /locus_tag="IVD64_001918"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVD64_001919"
     tRNA            1668..1741
                     /locus_tag="IVD64_001919"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IVD64_001920"
     CDS             1696..1845
                     /locus_tag="IVD64_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149167.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IVD64_001921"
     CDS             2338..3666
                     /locus_tag="IVD64_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6149168.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IVD64_001922"
     CDS             3868..4701
                     /locus_tag="IVD64_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP6149169.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IVD64_001923"
     CDS             4717..5454
                     /locus_tag="IVD64_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP6149170.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IVD64_001924"
     CDS             complement(5609..6577)
                     /locus_tag="IVD64_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP6149171.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IVD64_001925"
     CDS             6809..7642
                     /locus_tag="IVD64_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP6149172.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IVD64_001926"
     CDS             7676..8515
                     /locus_tag="IVD64_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6149173.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IVD64_001927"
     CDS             8541..9101
                     /locus_tag="IVD64_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6149174.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IVD64_001928"
     CDS             9300..11021
                     /locus_tag="IVD64_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP6149175.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IVD64_001929"
     CDS             11186..12328
                     /locus_tag="IVD64_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6149176.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IVD64_001930"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IVD64_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP6149177.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACBZN010000069        13749 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.069, whole genome shotgun sequence.
ACCESSION   DACBZN010000069 DACBZN010000000
VERSION     DACBZN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.069"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            107..2020
                     /locus_tag="IVD64_001931"
     CDS             107..2020
                     /locus_tag="IVD64_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6149178.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVEKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            2025..3026
                     /locus_tag="IVD64_001932"
     CDS             2025..3026
                     /locus_tag="IVD64_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6149179.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3228..3434)
                     /locus_tag="IVD64_001933"
     CDS             complement(3228..3434)
                     /locus_tag="IVD64_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP6149180.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(4080..4928)
                     /locus_tag="IVD64_001934"
     CDS             complement(4080..4928)
                     /locus_tag="IVD64_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6149181.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5092..5994
                     /gene="gnd"
                     /locus_tag="IVD64_001935"
     CDS             5092..5994
                     /gene="gnd"
                     /locus_tag="IVD64_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP6149182.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            5996..6997
                     /locus_tag="IVD64_001936"
     CDS             5996..6997
                     /locus_tag="IVD64_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6149183.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            6994..7278
                     /locus_tag="IVD64_001937"
     CDS             6994..7278
                     /locus_tag="IVD64_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6149184.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            7311..8108
                     /locus_tag="IVD64_001938"
     CDS             7311..8108
                     /locus_tag="IVD64_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6149185.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            8122..8955
                     /locus_tag="IVD64_001939"
     CDS             8122..8955
                     /locus_tag="IVD64_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6149186.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            8973..9830
                     /locus_tag="IVD64_001940"
     CDS             8973..9830
                     /locus_tag="IVD64_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6149187.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            9852..10361
                     /locus_tag="IVD64_001941"
     CDS             9852..10361
                     /locus_tag="IVD64_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6149188.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            10398..10799
                     /locus_tag="IVD64_001942"
     CDS             10398..10799
                     /locus_tag="IVD64_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149189.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            10911..11264
                     /locus_tag="IVD64_001943"
     CDS             10911..11264
                     /locus_tag="IVD64_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149190.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            11298..11741
                     /locus_tag="IVD64_001944"
     CDS             11298..11741
                     /locus_tag="IVD64_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6149191.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            11758..12843
                     /gene="uxuA"
                     /locus_tag="IVD64_001945"
     CDS             11758..12843
                     /gene="uxuA"
                     /locus_tag="IVD64_001945"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6149192.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            12836..13549
                     /locus_tag="IVD64_001946"
     CDS             12836..13549
                     /locus_tag="IVD64_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6149193.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         4752 a         2159 c         2957 g         3881 t
ORIGIN      
        1 taaaatgaga aaataatcct gttctatttt tatgggataa accatttatt ttttgtataa
       61 aaaatgtttg cgtttacata taattctcct agaaacataa aaaggagtga atagcttgag
      121 aacaaaacaa gtattaaagg gagcgttggt catcttattt gctgtaagta caagcgtccc
      181 gcaatggaca gtatatggag agagcatttc ttccgaatcg gaatctcggg aggtagaaac
      241 cacagaaact actgatgctg gaaatgaaac gaaatcgagt acttcacaaa cagaaacaaa
      301 tactgaaaca acggttgaga catctgttac tagcacagat caagaaacga ccgctaccac
      361 agataaccaa caaacagaag aagcaacagc tacaaaaatg tcaaacgacg aatcctcttc
      421 tgctaaagta agtgacaaag agaatgggac agaaaatgat gatatcggta aaggtgaaaa
      481 tttcaatggt aaagaatatt tgaattactt cggtaaacag atgacagaaa tacttaatgg
      541 accaacagaa gacatcaagt caaaattaat agagttatta gtcactgagc ttgctgttaa
      601 tgattatggt gtagaaaaag agattatcaa acatctgatt gctacttatc tattgtacga
      661 tatcgctttt gcaaaaaaca gtgtgttgat gtcgatgctt ggaggtacag atgatgggac
      721 aacgcctaca gaaaacacag gcttctatcc aaatacaaaa caacttcatc aaactgtact
      781 aaacaaacaa gatggcaaaa tgatggatgt atatgcatat tatgtcgatc aaggttcaga
      841 taaaacagtc atgatccatg gcggatttcg agggaattgg aataacggga tcgttacaga
      901 agaatacaat gatttttata aggccgggta taatttatta tttgttgatt ccagagcgac
      961 tggtaatagc ggaggcgatt atgtgaccta tggacaatat gagtcagatg acgtccttta
     1021 ctggataaat caagaagttc gagagcggcc atctcaaaag attttacttt atggtggttc
     1081 gatgggagca gctacgatga tgtctgtatt agcgaaagat attcctgtca atgtcaaagg
     1141 aattattgaa aattgcggat ttgcatcaat tgatgaacaa ctacgtttta cgtacagcca
     1201 gacagtagcc cccgcactac cggatgccat aaaaaatcag ttagacatca ttggggatca
     1261 agagcatgaa gatttattta tgggcctttt gaaacaatac tattttgatc aggagatgca
     1321 tttggataca aaagctgctc tacctacgat tggaatgtca gattcattgc ctaaattgat
     1381 tatccatgga acagcagatg atgtagtacc tgtgtctaac gcgcaaaaat tatatgagtt
     1441 atcaggaggg tataaagatc ttttattagt agaaggagca gggcatggaa aggcacagga
     1501 agttgaccat gctgcctaca cgaaacatgt aacggacttt ttgaaagtag tgttccatga
     1561 tcagataaac gtaaagtatg tggatgaaaa caataaaagt ctactcaaag accaggacga
     1621 aatccagctt tacggagctt atggagaaaa ttatatgaca gagcaaaaga cattcgaagg
     1681 atacgaatta gcaaatgttg aaggaccgac tgaagggatt tttaatgaga cgataccaac
     1741 tattattttc tattacaaaa aaatcccagt agctccacca aaaaagcaag atccgacaga
     1801 gaatgcagac aataagggaa agaatgaacc tcgagaggat caatcaagtg atgatacgca
     1861 accgattcag atgacagtga aagtacaaaa aaagacagat gcaaaaaaaa gaatgttgcc
     1921 gaaaactgga gaaaagaaac attcgcttct catttattca ggtggatttt tatctgttag
     1981 cacgattatc acatggattt ggcgaagaaa gagagggtaa atgaatgaaa tggttcaagc
     2041 gaatagtgat tgcagtacca gttatcatcg tagttatttt agtcactgta tttttgatta
     2101 atcaaatcac accaaagccg attagcttgc ttgttagaaa acaatttgac acaagtgaaa
     2161 aagaacaagt atatgcccga ccagatgatt tccagcaaaa aatcgaacaa atcgatataa
     2221 aaaaagaaat atcctatcct tcaaagtaca aaaataataa tatggatatc tacacaccga
     2281 aagtggaaaa gaaaaaatta ccgattcttt tctggatgca tggaggcgca tatgtaggag
     2341 gggataaaaa tgattgtcgt gattatttgg aatatctatg ttcagacacg cagcaaatca
     2401 tcgtaaatat agattatgaa cgatcaccgg aagcaaaaca tcctacacca gttatccaat
     2461 tgaacgaagc cattcaagca gctaaaaaag agataaaaga tcaagcagat tggtcgaata
     2521 tttcgattgg tggaaattcg gcaggtgcac aaattagtgg agaatatttg ttagcacttc
     2581 aaaatgaaga aattaaaaaa gctgatcatc ttgatccgac tttggagaac aagcagatta
     2641 cgaagtttat ttctttgtct ggtctgttgg atccttctgc acttactcaa gtaagtgata
     2701 aaatctctag ctttctttat gaaaaatgtg gatgggctta ctttgataat aaggactttg
     2761 aacaatcaga gaaggtcaaa agtttagcct tggtcagaca tgcggatcgt tggacgcaaa
     2821 acacattctt aacggatgga aatacaaata cgtttacgaa acaaatggaa gaagtaactt
     2881 ctgtatttga gaaagcgggg gtgaaagtca caaaagtaga ttatgcggag aaaaatgctg
     2941 ttttgaacca tgaatatcag tttgatttca gcaataaaca agcgcaagaa acatatcaaa
     3001 agttagtaat gtttttcaag gagtaaagtc ttatgatgca aaagaggttg taatctgtca
     3061 cttagctcca agtcacaagg agcaatttca ataaacaagc ttttcatggt ttatgaatat
     3121 tgtaacttgt gactgaggag ttagagatgg caaggggcac cttttattcg aaatttggga
     3181 atatcacacg attattgtca cgatctcttt tacgtaaatt ttataagtca gatttgttgt
     3241 tttgatcgtt taaaaaaagc aacaaatccg aagcctgcgg aggcaagaag ggacaaaaag
     3301 ccgagtggta agcaaaagaa tgctggttcc actaatattc cgttgttatt gatataaggc
     3361 tgggaacaaa gatgaatagc aaataaaatc aatgcactag cgaaaaaaag caaagaaata
     3421 tggatagctt tcataagcag cacctccttt tttataattg tatgataaat agattaagaa
     3481 tctataaatg tgacaagttt gttaggatgt gaataaaatg atcatctagc gaacttgaaa
     3541 caaagatggc taatgataag caattaatga ttcagcaatc agataggtac aaagtttacc
     3601 atcatacgtc tagaccaata atagaaaata tgggaatata atagaaaaga tttaggatac
     3661 ccccaaaaga atttccccaa aaattcgatg tatcctaaac ttttttattt tacataacaa
     3721 tcatatcaac tgtgaaataa aattacaaat gataacctaa aatgaggcca aaagccagtg
     3781 ttatcaagga aatacttaat tcaccaaaat tttttttaat tttttagaaa ataagaaaca
     3841 aaaataataa aaaaaagaaa agataaattt tttactactg tatactaact ttgttatcgc
     3901 atctaacaag taactcgaat aataccaata caaccttctc caaagatgcg atgacatacg
     3961 ttctaaccca ccgactattg atgttctgta ccacccttgc agaaccaaaa aagccactac
     4021 caaatatttg gtagtggctt tttttgtttt atggattttt cgctttttct gttatggtgc
     4081 tattctttga tttcagaaat agtatgtcga actttagcga tagaacgacg tgattctttt
     4141 tcgttgtaaa acatcaaaat cacatataga gagtccaaaa tgcttagttg agaaatacgg
     4201 gaagctaaag cttcagatcg gaattctgtt tcttcagaaa tagaaataaa aacgacatcg
     4261 ccaagtttag caagggaaga attagcttgg ctagtaataa cgattgtttt tgctccagtt
     4321 tttcttacgg tttcagcaat atggattgtt tcttttgatt gccctgtgtg agaaatcaaa
     4381 acagcacagt ctttttccgt caataaagaa gcttccatca attgaatatg gtaatccgtg
     4441 ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat aagcgtcagt tgctacaatc
     4501 tctgaaccgc caacaccgaa aaaatagaca cgtttagatt cattgataat agtagccgct
     4561 ttttttagat ccttttgttg taacaacttt tttgtgtcat ttaaagttga aatactagac
     4621 tcgaacacct tttgcgccat agtgagctca gtatctgttt tctgaatatt ttcgtggata
     4681 gaaatagcag atagatcatt ttcttggatc aacatggcca ttttaaaatc tttaaatcca
     4741 ttataaccaa gtttttttgt aaattgaaaa aaagtagagt cagcaatgcc cagttcactt
     4801 gctaaattgc taataggact atgacttact ttgcttggat tttctaaaat ataatcggcg
     4861 atagcctgtt cttttgggct gaagtcgttg tacattgttt tgatttgtag tcttatggag
     4921 acgctcatga gaattcctca ttttcataat aagattatca ttttttccgt tctcattaat
     4981 gttaaagata caaggagagt aggaaaaaat caaacaaaaa atatttttgt aaaatctatt
     5041 tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat catggaaatt
     5101 ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa agagcaccat
     5161 cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag agaaaatgac
     5221 attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa aaaaatcatc
     5281 tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact ttttccaaaa
     5341 ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga ttcaatccgt
     5401 cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac ttctggagga
     5461 atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga aacttttcta
     5521 tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta tgctggtgca
     5581 tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg aatgatgcaa
     5641 gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga cttggaacaa
     5701 gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga aatcatggaa
     5761 gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc tgcatcaggt
     5821 gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc agtaattgca
     5881 actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc aaaagtagtt
     5941 tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga gtaaaatgcc
     6001 agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc cacaccactt
     6061 ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag agattccagg
     6121 aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg ttacgccatt
     6181 ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc gacatggtat
     6241 tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg tatctatcat
     6301 acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt tgttgtccat
     6361 gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg aagatcgggc
     6421 ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg caggaaatac
     6481 cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc tagcttatga
     6541 tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag aacttcaaga
     6601 attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag agaactatca
     6661 catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat ctaatagtgc
     6721 aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg gaaagatcgc
     6781 aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc cgtatttagc
     6841 gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat gtttagaagc
     6901 aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc ttcctgaacg
     6961 ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt gaacggttta
     7021 aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc aaaagtttct
     7081 gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac ggtatcatgg
     7141 gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt tttttatcat
     7201 cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat catcaaaaaa
     7261 atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta gtgggttata
     7321 gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt aattgtgctc
     7381 catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg aaaacagaac
     7441 acttttacag acaagctgct ttttttataa cagatcccca attagtgatt gattcccctg
     7501 ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa tctgagctca
     7561 tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa gatactgcga
     7621 agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat atggcacaaa
     7681 gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt gctggtttag
     7741 ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg catgacgcta
     7801 tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta gcgtatggga
     7861 ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta tatcctttgt
     7921 atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg aaagataaac
     7981 agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat ttgattccta
     8041 tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt tatatcagaa
     8101 agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt gttgattgct
     8161 ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt gaccttacgt
     8221 ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt tgcacaagcg
     8281 atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc aggaggatca
     8341 atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt attaggggat
     8401 ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg aagttacttg
     8461 tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa agctgtagaa
     8521 gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt tgcctcattt
     8581 ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt gatcgctgat
     8641 gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt aggtggaggg
     8701 ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga ttatctggtc
     8761 tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat tacgatggtt
     8821 acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca aaaagggaaa
     8881 caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga agatgactat
     8941 gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat aatcaaacag
     9001 gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga tggcattttg
     9061 caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta tacggaatga
     9121 tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca tataaacggc
     9181 agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact ggcctaatgt
     9241 cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc ttgatgcagg
     9301 atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga atcggagatg
     9361 caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct tgggcacaac
     9421 agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat caggttttac
     9481 taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca ggcctaaagc
     9541 aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg tttatgatgg
     9601 gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg atatccggac
     9661 gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta cctttagctg
     9721 tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca caagcattat
     9781 tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa aaagaaggga
     9841 acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg atccatgggc
     9901 aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt gtagacgaag
     9961 ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc cgtacaggac
    10021 aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa tatcaattta
    10081 aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac ttagtaaaac
    10141 acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat cctgatacag
    10201 atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa ttgaaagaaa
    10261 tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct gcacctaaaa
    10321 caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct tagaaaaaag
    10381 ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa tttcgttatc
    10441 gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt tcagcattag
    10501 tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat acgctagcct
    10561 accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt atctgtactt
    10621 attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg aaagaccaaa
    10681 ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt ttaggttggt
    10741 ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta tccgtataaa
    10801 caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa gcagctcgcg
    10861 gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg atgctagaaa
    10921 tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa ggctgttatg
    10981 gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt cttttttatc
    11041 tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt tcaaataaag
    11101 aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat atttttagtg
    11161 caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga gggaaaacat
    11221 ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa agtgcaaaac
    11281 aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag ttgagcgaag
    11341 gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa acaatgtgct
    11401 tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa ttaactatga
    11461 tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt ccgtacaatc
    11521 aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact tatgtcatag
    11581 ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt cacacaccga
    11641 tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact gtttggcatg
    11701 cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc tgaaaagatg
    11761 aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa atatgttcgc
    11821 caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc tggagatgtt
    11881 tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg actggaactg
    11941 cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga tgaacgagat
    12001 tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg ggttcgattg
    12061 atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt taatgaaaac
    12121 aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat ggagccagga
    12181 gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg ttgggaagaa
    12241 gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac cgaagaagaa
    12301 ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga aaaagcagat
    12361 gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt tcctagagtg
    12421 acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc atataatgga
    12481 ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt tgaaatcatc
    12541 catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt tttaggagaa
    12601 agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat gtatcatatt
    12661 atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca tggtagaacc
    12721 gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat gggactcact
    12781 tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga gcattatgaa
    12841 taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg gacgtttagc
    12901 ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta aggaagtcta
    12961 ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca ttttgtacgg
    13021 agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag aaaaaatcgt
    13081 gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat cttttgcaaa
    13141 agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag tagaaatgta
    13201 tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg atgatgtaaa
    13261 aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga atattcctcc
    13321 aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa ggcgagcggg
    13381 cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata atgggaagca
    13441 taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg aattttatga
    13501 cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat aaagaggact
    13561 attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg aaaaagaagt
    13621 aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac aaacacaaaa
    13681 cgcatctact gttatctacc agtagatgcg tttaaagaga agaacgagaa tcgtccatat
    13741 gagggtttc
//
LOCUS       DACBZN010000070        13429 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.070, whole genome shotgun sequence.
ACCESSION   DACBZN010000070 DACBZN010000000
VERSION     DACBZN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.070"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..914)
                     /locus_tag="IVD64_001947"
     CDS             complement(<1..914)
                     /locus_tag="IVD64_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP6149194.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IVD64_001948"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IVD64_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP6149195.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IVD64_001949"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IVD64_001949"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP6149196.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IVD64_001950"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IVD64_001950"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP6149197.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IVD64_001951"
     CDS             4440..5321
                     /locus_tag="IVD64_001951"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP6149198.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IVD64_001952"
     CDS             5318..6034
                     /locus_tag="IVD64_001952"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP6149199.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IVD64_001953"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IVD64_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP6149200.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IVD64_001954"
     CDS             6751..7752
                     /locus_tag="IVD64_001954"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP6149201.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IVD64_001955"
     CDS             7749..9110
                     /locus_tag="IVD64_001955"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP6149202.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IVD64_001956"
     CDS             9070..10221
                     /locus_tag="IVD64_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP6149203.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IVD64_001957"
     CDS             10196..11365
                     /locus_tag="IVD64_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6149204.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IVD64_001958"
     CDS             11540..12919
                     /locus_tag="IVD64_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6149205.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IVD64_001959"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IVD64_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4485 a         2339 c         2923 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgagaaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACBZN010000071        13404 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.071, whole genome shotgun sequence.
ACCESSION   DACBZN010000071 DACBZN010000000
VERSION     DACBZN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.071"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            79..1125
                     /locus_tag="IVD64_001960"
     CDS             79..1125
                     /locus_tag="IVD64_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6149206.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IVD64_001961"
     CDS             1474..2106
                     /locus_tag="IVD64_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP6149207.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IVD64_001962"
     CDS             2694..4133
                     /locus_tag="IVD64_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6149208.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IVD64_001963"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IVD64_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IVD64_001964"
     CDS             4895..5110
                     /locus_tag="IVD64_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149209.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IVD64_001965"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IVD64_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IVD64_001966"
     CDS             complement(5770..6681)
                     /locus_tag="IVD64_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6149210.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IVD64_001967"
     CDS             complement(6700..7704)
                     /locus_tag="IVD64_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6149211.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IVD64_001968"
     CDS             complement(7701..9824)
                     /locus_tag="IVD64_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6149212.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IVD64_001969"
     CDS             complement(9829..12276)
                     /locus_tag="IVD64_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6149213.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IVD64_001970"
     CDS             complement(12263..12652)
                     /locus_tag="IVD64_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6149214.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IVD64_001971"
     CDS             complement(12712..13215)
                     /locus_tag="IVD64_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6149215.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..>13404)
                     /locus_tag="IVD64_001972"
     CDS             complement(13228..>13404)
                     /locus_tag="IVD64_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149216.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4099 a         2643 c         2454 g         4208 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaacccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DACBZN010000072        13397 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.072, whole genome shotgun sequence.
ACCESSION   DACBZN010000072 DACBZN010000000
VERSION     DACBZN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.072"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(14..706)
                     /locus_tag="IVD64_001973"
     CDS             complement(14..706)
                     /locus_tag="IVD64_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6149217.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IVD64_001974"
                     /pseudo
     CDS             791..967
                     /locus_tag="IVD64_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IVD64_001975"
     CDS             complement(1143..1712)
                     /locus_tag="IVD64_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6149218.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IVD64_001976"
     CDS             complement(1786..2109)
                     /locus_tag="IVD64_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149219.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IVD64_001977"
     CDS             complement(2263..3075)
                     /locus_tag="IVD64_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149220.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IVD64_001978"
     CDS             3429..4277
                     /locus_tag="IVD64_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP6149221.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IVD64_001979"
     CDS             4422..5363
                     /locus_tag="IVD64_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP6149222.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREIPFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IVD64_001980"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IVD64_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IVD64_001981"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IVD64_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP6149223.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IVD64_001982"
     CDS             complement(9827..12013)
                     /locus_tag="IVD64_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP6149224.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IVD64_001983"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IVD64_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP6149225.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IVD64_001984"
     CDS             complement(12235..12675)
                     /locus_tag="IVD64_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP6149226.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IVD64_001985"
     CDS             complement(12749..13288)
                     /locus_tag="IVD64_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6149227.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3762 a         2810 c         2382 g         4443 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatccc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaata
//
LOCUS       DACBZN010000073        13343 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.073, whole genome shotgun sequence.
ACCESSION   DACBZN010000073 DACBZN010000000
VERSION     DACBZN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.073"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            5..763
                     /locus_tag="IVD64_001986"
     CDS             5..763
                     /locus_tag="IVD64_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6149228.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(849..1589)
                     /locus_tag="IVD64_001987"
     CDS             complement(849..1589)
                     /locus_tag="IVD64_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149229.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            1650..2903
                     /locus_tag="IVD64_001988"
     CDS             1650..2903
                     /locus_tag="IVD64_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6149230.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            3225..3452
                     /locus_tag="IVD64_001989"
     CDS             3225..3452
                     /locus_tag="IVD64_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6149231.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            3575..3901
                     /locus_tag="IVD64_001990"
     CDS             3575..3901
                     /locus_tag="IVD64_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6149232.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            3969..4682
                     /locus_tag="IVD64_001991"
     CDS             3969..4682
                     /locus_tag="IVD64_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6149233.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            4992..5843
                     /locus_tag="IVD64_001992"
     CDS             4992..5843
                     /locus_tag="IVD64_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6149234.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            5964..7829
                     /locus_tag="IVD64_001993"
     CDS             5964..7829
                     /locus_tag="IVD64_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6149235.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            7877..9286
                     /locus_tag="IVD64_001994"
     CDS             7877..9286
                     /locus_tag="IVD64_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP6149236.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            9394..10095
                     /locus_tag="IVD64_001995"
     CDS             9394..10095
                     /locus_tag="IVD64_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6149237.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            10254..10733
                     /locus_tag="IVD64_001996"
                     /pseudo
     CDS             10254..10733
                     /locus_tag="IVD64_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            11581..12156
                     /locus_tag="IVD64_001997"
     CDS             11581..12156
                     /locus_tag="IVD64_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP6149238.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            12156..12596
                     /locus_tag="IVD64_001998"
     CDS             12156..12596
                     /locus_tag="IVD64_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6149239.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            12612..13313
                     /locus_tag="IVD64_001999"
     CDS             12612..13313
                     /locus_tag="IVD64_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6149240.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         4466 a         2203 c         2820 g         3854 t
ORIGIN      
        1 agaaatgaaa aaaacaattg cattaagtac cgttctagct ggaagtctat tattatgtac
       61 acaaagtgtt tttgctgaga ataagagctc agccactaca gaaggaacga tttctgtgac
      121 caaaccagat gctgcaaatc ctgaagacgt tgttccagtt cctcctgttg atccaaatga
      181 tcctgatact ccttttacac ctgacgaacc aaatcaggga acatccggat tactgacgat
      241 tgaccaagct ccagatcttg attttggaac aatcaagtta ggtacagcaa aaacagtata
      301 tgctgcatta caaagcggaa cggacagtac tggagcacaa acacaagtac agaatttcgt
      361 acaagtaacg gataaaagcg gaaattatgc cggttggaaa ttatcagtca aacgaacaga
      421 atttattggt acaacatctg atacgaataa actaactggt tcaaaggtga aattcaaaaa
      481 cgccgtgatt cgaaatagcg caactaatag cagtggaatg ccatctaccg tttctggaac
      541 tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta gatctagtaa ctgcaggagc
      601 aaatgaaggg attggcacat ggatttattc attaggagag aatgtggaac aagggagaca
      661 atcagtggaa ttatttgttc ctgtatccaa ttatgctgta gacaactacg tttcaacatt
      721 cacatggtcg ttaacagatg ctcctacaga agaggaagcg taaaatcata tcaacgattt
      781 cagggagtgg agccgattca aggcccactc cctgaaatct gctatgctta cgtctcagct
      841 cttattcatc aactcgaagc agttttttgg aagtcatgga aacgttcctt tgtcttttca
      901 aagatctttc gaccaacgag taaggagtca ccgttgaaaa attctaacac ataatgagca
      961 gaatcaattt tctgcaattg cgataagttg atacagtaag attttaaaag taaaaagtaa
     1021 gcatactttt ctaactctgt cttcaattgt tttaaagaag tattcactaa tttatcttct
     1081 gtagttgttt tgaccataac ttggtttttc aaacctttga tactttcaat atataaaatt
     1141 tcccgaggat acaggacgag acttccacct ttttgatcaa taaagatttt atcgacattt
     1201 acgatctgct gttctttcca attgatctgt tctagcactt gacgaatttg ttcagataaa
     1261 ttcctgtctt ttctgatata tccaatcgga aggattttcc gattgatgat ctctactgca
     1321 aaattcgtat aagcggtgaa aaaaacaata ttactatgtg catttttttt acgtatttgt
     1381 tcggcgacat caatacctgt gagtgattgc ttcaagtcga tatcgataaa gaaaacatcg
     1441 tgattatcaa tagtgcccaa ctggatcaac tgattgattt gagagataga agtgagctgc
     1501 acgatattct cgatatgatg attgaccaat tctctgatga tccattcgct gtgaaactgg
     1561 ttatcttcta tgataaaata ctttttcata tgcaggctcc tattaaaaaa atatgtgtac
     1621 attttgaaac aaaacaatgg aggaataaga tggtcgcaac agtgacactt tatttgacct
     1681 ttactttgat tttagattta ggtatctatt tattcttttc gaaacaactg ctagtaaaaa
     1741 acagctttgg ttgtatagct ttgatggtgg tgacactttt acttcctatc cctaaagtga
     1801 gctttcctta cctagttggc ttattattga tgtttctttt ttattttact acgaaaaacc
     1861 tgtttatcag ctgtttgatc agtaatcttt tttttgctat cactggactc agcttttttc
     1921 tggtttttga tctgatggat tatttaggaa agagacatct tggaacagca agtttttttg
     1981 cactacttat cgcatttatt ttctatctat tagtaaaaag tatagacaaa cagacgagat
     2041 ttctcaactt gctttttact tatccaactg aacactattt tggaggtatt tgtattttat
     2101 taagctgggg cgtgatttac ttggtattgg gaagtgttca cttccgctca tctaattatc
     2161 tgcttctatc tcttttgtcg atagtcatca gttttttttc tttgtttttt tccattatgt
     2221 tgatcagttt ccaagtaaaa gaaagacaac atcaggaatc gcttcaactg tatcaaataa
     2281 atgaagaata ttaccatcat ctagaggaat tcaaacatga ttataaaagt ctactgtttt
     2341 cattaaaaat tactcttcag gaaaataaaa aagaaacgtt ggaattttta gaaatattag
     2401 aagatcattc tgcagctatt tttgaaaaac cgcaaataca acagctaaca aatattgtat
     2461 ccccagctgt caggggtgtc tttttaaaat ttctagaaaa agcagaatcg gagaaaatcc
     2521 cgatccaagt agttattcct gtcgaagtga aaacaatccc gattgatctg gttgttttta
     2581 ttcgctgttt atctattttt atttctaacg cattcgatca tcgtgtagcc gatcagagtc
     2641 cgattacgat caatctgctg gaagaaaaag agggatcacg ttttagtata gcgaacaagg
     2701 caaatccaac gaatttgacg ctatcggaga tggtcaaaag aggaaaaacc tctaaaaaaa
     2761 atggcgggtt aggtctatac agtgcgaaaa aaatgttgga tgtttacgaa aatgcagaac
     2821 taaaaatcga attttcaaaa cagaatcact atttttttgt agaaatgtat ctggcatcta
     2881 gaaagagcaa atcagcctac tgacttactg tcaaaggata agtttgagga aagtctacac
     2941 ggagatagag acaacaacac agcatattca aaagaaacag aagcgtgatg accgttgtca
     3001 cccttctgtt tcttttgcta tgtagttaaa caaagtagtt gacacttccg ctacttagta
     3061 ttagtacgaa agtatacttc atgatactaa gtagataaga agtgaaaagc ggaacccacg
     3121 atcgtcaaaa tgctaactgc tttgcttaca ccagataaag ggaagagcaa tatcgcttat
     3181 tttaggcgtg gagaagcaag caaatcccta cgatcacaaa aaaaatgctg acccagcagt
     3241 taagagagtt agaacaagat gaaagtatcc atcgaaaagc ctataaccaa gtcccgccta
     3301 aagtagagta ctcgctcacc gaagaaggaa aatcattaag agagattcta gtagcgttgt
     3361 ctgtttggga agaagaacgt atcgaaaaac aaaaagcctc aagggaagaa gacacgctac
     3421 tgcatacaca tggtggctac ctaaattatt gattttctat gacatttatt ttaaataaaa
     3481 gaaaacagga gtttttttaa aagcatcatc tattttttat gtaaaaaaat ggtatgatta
     3541 atcaagtaag aagtgattgg aagaaggcaa gcaaatgaat agtatggaaa aagtcaacca
     3601 gtttagagat gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg ctatctctat
     3661 ttctttggaa gcatctgaat tgttagaact ttttcaatgg aaaacgccgg aagaagtaaa
     3721 agaaacacaa ttagaacgaa tcaaagaaga actggcagat gtattgatct attcatacat
     3781 gatggcagac aaccttcacc tggatctaga tgagatcatt gaagaaaaat tgatcaaaaa
     3841 taatttgaaa tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca cagagttgta
     3901 gaaatgtgtc attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg agtctggagg
     3961 gagagaaaat gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc ctcttgttcc
     4021 tcttgttatt agtaggacta gctttgattt tcaatgaaca gatcaaggat tattttgtca
     4081 gagagacagg agataaatat gctatagcga atgtaacaaa agaagatctg aaaaagaata
     4141 acgataaaga tgtcagtttt gactttgatg cggtagaacc aatgacgaca gaaggggtca
     4201 tgaggtcgca aatgagagga acagatctac cagtgattgc tagtatcgca gtcccttcag
     4261 tttcaatcaa tttaccggtt ttcaaaggct tggataatac gtcactactt tacggagcag
     4321 gaacattatc tccagatcaa gaaatgggaa aaggaaatta tgccttagca agccatcgtg
     4381 caacgaaccc tgaattgctg tttacaccgc tagaaaattt agagatgggt gccaagatct
     4441 atttgacaga tttagaaaat gtgtatacat acaaaacatt ttttaaagaa aaagttgctc
     4501 ctacagatac ccaattactg aatgaagtcg agggaaaaga aattgtcaca ctaattactt
     4561 gtggcgacat ggacgccgtc acgcgactag ttgtacaagg agaactggaa agtgttacat
     4621 cgataaaaga tgcgacagat gatatgagat ctgctttcaa tctagagaca aaaacatttt
     4681 aggatactgg gacataagtc gttacagatt aaaaacagcc aaaggatatg aatcgataag
     4741 gaaaaaccaa tttgctgaaa aaaagagaaa cgcaaagagg tgcatctctg atttggttgt
     4801 tttctagata gacttgtgtt ccagtttttt tattttttat atgaaaaggc ttgcataaat
     4861 aaaaattcga tggtatgatg attttgaata agaaatggat tgttactgta cggcaggcaa
     4921 aaccagaatt cagctaagaa acagtaggcg aattctaggt ttttttattt ttcttgagag
     4981 ggcgaagcaa aatgaaaatt gataaaattt tgaataacaa tgtcgtcatt tcgaaaaatg
     5041 gattcgggga agaagtcgtt tgtatgggaa aaggactagc ttttcaaaaa aagattgggg
     5101 acgagatttc gccagaagcg gtccaaaaag aatttgtttt aagagattct tttgctaaaa
     5161 accaattcca gcagctgatg gctgatattc cggcagagga gatcgaatgg gtcaaacaag
     5221 tagtcgaact ggctgaagat aaactaaaag ttgaattttc gcctaatatt tatctgacat
     5281 tgaccgatca tcttcattat gcgatcacac gagcaaaaga aaatatccaa ctgcctaatc
     5341 cattattatt tgaaacaaaa aaattttatc cgaaagaata tcaagcagcg aaagaggctc
     5401 ttttgttcgt gaaacaaaag acgggtgtgg aactttctga ggatgaagcc ggttttattg
     5461 cctttcattt tgtgaatagc caacaaggaa acgaagacat gcaagtgacg atgaccgcta
     5521 ccacaatggt aagagatata ctgagtatca tcagtaaatt cttcggcatc gtgttcgatg
     5581 aagaatcttt gaattatcag cgtatcatta ctcatctgca gttttttgcc cagcgttatc
     5641 tcaaagggga aggatctgac gagcaagatg aatttctcta cgcattagtt caaggaaagt
     5701 atccaaaagc gtttcattgt gtcgaacgga tcaatgagta tcttcgacaa acgcaaaaac
     5761 aggaaatggg gatcgcagag cagatttatt taacgattca tattcaaaga gtagtaagtg
     5821 agaaaaaaag catccaatca taaggattgt tactgtatgg caggcaaaac ctggattgaa
     5881 aaaataggtt cacgaatgtt tgtgactgtt tttttcaatc tgggttttta ttttttaaat
     5941 aaaaaagaaa gaaggaaaaa acaatggata atcaagcagt tggtcgtcgt gtatgggaag
     6001 cggtaggagg acaaaagaat gtcaaaagtt tagtacattg tgctacgcgg ttgcgattca
     6061 gattaaaaga tgaatcttta gcagatacgc aaaagttaaa agaagacccg gacgtgatcc
     6121 aagtcgtaca aagtggcggt cagtatcaag tagtgattgg cagtaatgta gcagatgttt
     6181 atcaatcgat cgtggatgag gaaggtcttg cagtttcgga ggatggaaaa gaagagtcca
     6241 aaaatatttt gaatcgtctg atcgatatta tctctagtat atttacacct tttctagggg
     6301 cgatggcagc cgccggggta ttgaaaggat tcttatcatt ggcaacggtg atgggctggc
     6361 tgacagctga tagcggtact tatcaaatat tgtttgctgc agcagatggt gtctttacct
     6421 tcttacctgt catgcttgct tttacagcag ctaaaaagtt caaagccaac cagtttttag
     6481 cagtagcaat tgctatggca cttgtgtatc cagctatcac agcagttgct ggtgcgggag
     6541 aagctatcag tttctttggg atcccagtga ttctttcacc atctggttat acttcttctg
     6601 tcataccaat cattctagcc gtatgggttc aaagtaaatt agagccattc gtaaaaaaag
     6661 tgattccgca atttctgcaa atgattttag ttccgcttgt cgttttagta gtgatggttc
     6721 cattaacatt tcttgcttta ggaccaatcg ggacggttgc cggtaatgct ttaggcggtc
     6781 tcttcaactc gatttatgga tttagtccaa tcgtagcagg tctaatcatg ggaagtttat
     6841 ggcaagtatt cgtgatgttc ggcatgcatt ggggattcgt accgatcatg tttttgaata
     6901 tcgaacaata tgggtttgat gtattgatgc caatgcttct tccagccatc ttggcacaag
     6961 gtggggctgc tttagctgta gcattgcgaa caaaagatac gaaacttcga gcattaggga
     7021 tttcttctac agtgacttcg ttatttggaa tcactgaacc aactgtgtat ggtgtgacat
     7081 tacctttgaa gaaaccattc attgccgctt gtatttctgg aggtattggt ggagctatta
     7141 tcggattttc cggagtaaaa gctttctcca gcagtttggt cagcttattg acgattccga
     7201 ctttcattaa tacggtggat ggtgtagagt caaatgtgac agtagcagta atagctacag
     7261 gaattgcttt tgttcttgca ttcgtaggta cattgatttt aggatttgac gaacaaacac
     7321 aagacaatca attagaaaac aaacatgcga atgcaggaga accgattact tcagcacggc
     7381 ataccttaaa aagtccttta acgggtaaag tactgccatt gagtgaagta ccagaccaag
     7441 ttttttcttc tggcgtgatg ggaaaaggta tcgctattga tccagaagta ggagaactag
     7501 ttgcacctgc agacggagag atcacgacta tttttccaac tggacatgct gtagggatca
     7561 caacgactga cggagcggag attttgattc atatcgggat ggatacagta gaattaaatg
     7621 ggaatggctt tgagatctta gtcaaacaag gggatctcgt caaagcagga gatttattga
     7681 ttcgttttga catcgaagca attagagcag ctggctatag cgtaatcact ccagttgtca
     7741 ttacgaatac tgatgcattt gccgatatac ttgaattaga ccaaaaagag attattgcaa
     7801 acgaagatgt tctggcaatt gtaaaataag gaggaaatca ggatgcaatc acgtttttca
     7861 gaaaaatttt tatggggtgg agcaactgct gccaaatcaa ttagaagggg cttatgatcg
     7921 agacggaaaa ggactatccg ttgcagatgc tatgcctgga ggtaagcagc gctttgcgat
     7981 tatcggcagt gaagaatttg actggacgat tgatcaagaa aaatatattt acccgaatca
     8041 tcgaggaatc gatcattacg atcgtttcaa agaagatctt gccttgtttg cggaaatggg
     8101 attcaaatgt tatcgttttt cgattgcctg gacacgaatt tttccaaatg gagacgaagg
     8161 tactcctaac gaagcgggat tagaatttta tgatcagctg attgatgagt gcttaaaata
     8221 tgatattgaa cctgtgatca cgatttctca ttacgaaatg cctcttcatt tggcaaaaga
     8281 atacggtggc tggaaaaatc gaaaactgat cgacttctat gaacgatttg ctcagacagt
     8341 attagagaga tacagcagta aagtcaaata ttggatgact ttcaatgaaa tcaattcggc
     8401 ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc aatggagcag gagagtacca
     8461 aaatattttc caagcatggc ataatcaatt cgttgcaagt agcaaagccg taaaaatcgg
     8521 ccacgaattg cgttcggata tccaaatcgg ctgcatgatt atctatgcaa caacttacag
     8581 catcgatgcc aatccagtca atcaggcagc aacaatgatc caaaaccaag aattcaattt
     8641 tttctgcacg gatgtccaag tacgtggaga ataccctgca tacacagcgc gtacccataa
     8701 aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc agttgctaaa
     8761 agaatatcca gtggactaca tcggatttag ttattatatg tctacggcaa tcaatgagac
     8821 tgatccacaa gcagcaactt cagaagggaa cttgctggga ggagtaaaaa atccgtttct
     8881 tgaggcaagt gaatggggct ggcagatcga tccagaaggg ctgagaatcg cattgaatga
     8941 attatacaat cgttaccaga aaccattatt cattgttgaa aacggtcttg gagccattga
     9001 tcatatggaa gaggatggca cgattattga tgattatcgg atcgattatt tacgtcgtca
     9061 tatcgaagcg atggctaatg cagtagcaga cggtgtagat ttgatgggct atacgccttg
     9121 gggatgtatc gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt acggctttat
     9181 ctatgtggat ctagacgatg aaggaaatgg gacactgaat cgatcaaaga aaaaatcttt
     9241 tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa agcagctgaa
     9301 tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt ttttattttt tatgtgctat
     9361 tcttttagta atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt actatacttg
     9421 gaagtcgcag ataaaattaa agaagatatt ttctcaggaa aatatccagt cggttcgatg
     9481 ttgccgacag aaagtgaatt agaagaactg tttcaagtca gcaaaatcac tgtccgaaag
     9541 gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg tgggacgacg
     9601 gttctaagca atcgtcctta caatcgatta tccaaagcgg caagctttac gcaaatcttg
     9661 gagaactccc gatatgaagt ccggaaagaa acgctggatt tgcaattggt ccatataaag
     9721 aaggaagatc ctttgttttc ctttttcggt gagcaagctg tttgttttaa gcgcttatat
     9781 tatttgaaca atcagcctta tatttatttt gttcattatc ttccacaaga aatgatgcaa
     9841 ttcagcagag aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa aaaaggatat
     9901 actattgact cattttctga tgatttctcg gcagtatttt taacagaaga agaacaagga
     9961 attctgaaaa cagacgcaca gatcggcatc aaacggatac ggaaatcgtc tacgcaaatt
    10021 ggggaaatga ttgaatattc ggaggcggtc taccatacgg agctgcatcc ttaccatatt
    10081 gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc tgccctgcat caagtaataa
    10141 gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttaggctta ttaccgcaga
    10201 tacacggctg tttctggatt accatttatt ctggataagg gggtgtggca cgattttcag
    10261 aaggaatcta tgaaccaatc actgtattga ttttagcttc gatggtaacc ccattcggct
    10321 tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa tatactcaat cgtcaagaaa
    10381 tcgatacatt gaagaccgct tttcctatgg ggatctgtca gatcacagag ggatgtttcc
    10441 ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac gggagtcggt gggacagtag
    10501 gtggcggatt aagcatgttt tggggcgcag atagccgtat tccggcatcg ggaatgtttg
    10561 cagtcgctac gatgaccaga ccatgggcat tcattggtgc attgttagct ggttcttttg
    10621 tcacaggaat aaccatttta ttattgaaaa aaccagtaga tccgaatgca gaaatcatcc
    10681 aagaagaaaa agaggaagaa gacatatctt gggatgattt gacaattagt taggagaggc
    10741 agaatggatt acaagaaaaa agtacaatag atgtaccttg agacaggaat ggtatgaaag
    10801 aatcagagtt agaaagaatc gaatatacag attttggttt aggagcgatc gaaactgtcc
    10861 agagcatcgg caccctcctt gaaatgacga tgaaggaaaa atgagaacct ttcgttgtcg
    10921 aaagggtctg gtatacctta tgtcgaagga aaggaaacaa aaaaactttc ctgtactatt
    10981 ccaaaaagga aagaaagtta ttatacagta aaaaaagaaa ttattcttca tgctagagat
    11041 cagtacacgt tcgagcccaa tatcaaacac tagttccagg caggcgcaac aggtgcggtt
    11101 atcactgaat tttcttccag cagtgacgat tcaagtgata tttttacaaa tccagctatt
    11161 tatcgttaac aaaaagattt ttaggagaag ctgatgaaca aaaatgatca aattttgatc
    11221 gtttagttcg tcggcttttt tatttagaga aacatcattt atcagtgaaa cataacacga
    11281 gaacaaaatg attttttcgt taataaaaga aacaagtatt tgttgtttaa aataatacac
    11341 gttattggca tataggacga aatctcagta tcttctataa gtaagtcatt tcatatcgat
    11401 gaaaacacgt tatttatttt tgttttatct ataaaatggc tgaaaaatga tttttttcta
    11461 aatactatat atagtggcgt ctatcctata taccactaaa aaactacaaa atctagtctt
    11521 ttattttaaa aaaacacgtg tagaatgaag atagactttt gagttaggga gtgttttttt
    11581 atgaaactta ctgttttttg cggttcccgt tttggtaata aagaaagtta caaatttatt
    11641 gcgcaaacgt taggaaaata catggcacaa gaagaaatcg aactggttta cggtggttca
    11701 tactccggga ttatgggagt gattagtaaa acagtactgg aaaataacgg aaaagtcaca
    11761 ggtatctatc cgaatgggct ttttgaaact gaattgcctg gaaaagatgt tactacattt
    11821 attcctacag aaacgatcga tgaaagaaag gtattattat ttgaaaaggg agatgctgtc
    11881 ctcgttttcc ctggaggtct aggtacattg gaggaattct cacaacttct ttcttggata
    11941 gcaatcggtc tgacgcccga caagccaatc ggaatattga atattggcgg ttattacagc
    12001 ggcttgcaac aattattaga aacttttgca aaagaggatt ttatggataa aaaatggttg
    12061 gatcaagtaa tcttttccaa caatccattg gaactggtca atgaattacg agcagtatct
    12121 attaaaaatc agttattatt gaaggaggca aaataatgag tcaaatatat ttagcaggac
    12181 catttttttc agaggagcaa atcgatcgag taagtcgtat cgaaaaagcg ttggaggaaa
    12241 acaaaacagt cacaagtttc tactctccac gtcatcacca agaaagcaat tatgaactat
    12301 tcagcgctgg atgggcgcag gaagtatacg aaaaagatat ggaagaactg acaaatgcag
    12361 aatttgttgt tgctatcctt gactttgagc atcaaacaat cgatccagga acggcttacg
    12421 aattaggtgt ggcaactatg ttgaaaaaac cgatgatcat tgtacaagaa gagacggtac
    12481 ctacgaactt gatgattaca caaagtctcc atacgtattt aaaatcagac caagctgtac
    12541 gtgaatatga ttttgaaaca ttgcctgttg aaacttacgt aggcgagtac ttatagaaga
    12601 ggaagggttg tatgtcagtg attttgattt caggaacgat tggtgcaggt aagagcagtt
    12661 tgacggatat gcttgcaaaa gagattgatt ctaaaccatt ttatgaaaat gtcgaagata
    12721 acgaagtatt gccgctcttt tattcaaatc ctgagcagta cgctttttta ttgcaaatct
    12781 tctttttgaa caagcgattt ttagcaatga aaaatgcttt agtgaacgat gacaatgtgc
    12841 tagaccgatc catctatgaa gatagcctgt tgttccatct aaacgctgat ttaggtcggg
    12901 taacggatat cgaagtacag cagtacgata gcttattaga tacgatgcta aacgagttag
    12961 atgatgttgc tccaaaaaaa cggcctgatt tgatggtcca tatcaaagtt tctttggaca
    13021 cgatgctaga acgtatcaaa aaacgtgggc gtgactatga acagctagaa agcgatgaga
    13081 cgttatatac ctattatgaa acgttgaaca cccgctataa tcaatggtat gaagattttg
    13141 atgtatgtcc taaaatacaa gtggatgggg ataaattcga ttttgtagaa aatccagaag
    13201 atgcaaaaaa ggtgatccgt cagatccaag aaaaattagc tgaattggaa ggaaagcctg
    13261 ctggaaaata ttttacggat aaccagcatg caaaaggtca agaattaggc taaaaataaa
    13321 aaaccaaacg acgggaaaga aaa
//
LOCUS       DACBZN010000074        13333 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.074, whole genome shotgun sequence.
ACCESSION   DACBZN010000074 DACBZN010000000
VERSION     DACBZN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.074"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IVD64_002000"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IVD64_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP6149241.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IVD64_002001"
     CDS             complement(738..1460)
                     /locus_tag="IVD64_002001"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP6149242.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IVD64_002002"
     CDS             complement(1596..2477)
                     /locus_tag="IVD64_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP6149243.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IVD64_002003"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IVD64_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP6149244.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IVD64_002004"
     CDS             3717..4196
                     /locus_tag="IVD64_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP6149245.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IVD64_002005"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IVD64_002005"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP6149246.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IVD64_002006"
     CDS             5497..5625
                     /locus_tag="IVD64_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6149247.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IVD64_002007"
     CDS             5629..6174
                     /locus_tag="IVD64_002007"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6149248.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IVD64_002008"
     CDS             complement(6628..8319)
                     /locus_tag="IVD64_002008"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP6149249.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IVD64_002009"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IVD64_002009"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP6149250.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IVD64_002010"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IVD64_002010"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP6149251.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IVD64_002011"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IVD64_002011"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP6149252.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="IVD64_002012"
     CDS             12601..13302
                     /locus_tag="IVD64_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6149253.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3751 a         2710 c         2368 g         4504 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taa
//
LOCUS       DACBZN010000075        13173 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.075, whole genome shotgun sequence.
ACCESSION   DACBZN010000075 DACBZN010000000
VERSION     DACBZN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.075"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVD64_002013"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVD64_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6149254.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IVD64_002014"
     CDS             1995..4661
                     /locus_tag="IVD64_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6149255.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IVD64_002015"
     CDS             5025..5777
                     /locus_tag="IVD64_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6149256.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IVD64_002016"
     CDS             complement(5799..6953)
                     /locus_tag="IVD64_002016"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP6149257.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IVD64_002017"
     CDS             7138..9579
                     /locus_tag="IVD64_002017"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP6149258.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IVD64_002018"
     CDS             complement(9909..10613)
                     /locus_tag="IVD64_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149259.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IVD64_002019"
     CDS             complement(10800..11045)
                     /locus_tag="IVD64_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149260.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IVD64_002020"
     CDS             complement(11062..11913)
                     /locus_tag="IVD64_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6149261.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRALEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IVD64_002021"
     CDS             12079..13083
                     /locus_tag="IVD64_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP6149262.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4204 a         2340 c         2751 g         3878 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaagtgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaa
//
LOCUS       DACBZN010000076        13076 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.076, whole genome shotgun sequence.
ACCESSION   DACBZN010000076 DACBZN010000000
VERSION     DACBZN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.076"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(285..2069)
                     /locus_tag="IVD64_002022"
     CDS             complement(285..2069)
                     /locus_tag="IVD64_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149263.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(2069..3820)
                     /locus_tag="IVD64_002023"
     CDS             complement(2069..3820)
                     /locus_tag="IVD64_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6149264.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(3962..4186)
                     /locus_tag="IVD64_002024"
     CDS             complement(3962..4186)
                     /locus_tag="IVD64_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP6149265.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4437..6023
                     /locus_tag="IVD64_002025"
     CDS             4437..6023
                     /locus_tag="IVD64_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP6149266.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6089..7864)
                     /locus_tag="IVD64_002026"
     CDS             complement(6089..7864)
                     /locus_tag="IVD64_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP6149267.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8077..8979)
                     /locus_tag="IVD64_002027"
     CDS             complement(8077..8979)
                     /locus_tag="IVD64_002027"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP6149268.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            9258..11603
                     /locus_tag="IVD64_002028"
     CDS             9258..11603
                     /locus_tag="IVD64_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6149269.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(11686..13026)
                     /locus_tag="IVD64_002029"
     CDS             complement(11686..13026)
                     /locus_tag="IVD64_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6149270.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3933 a         2645 c         2462 g         4036 t
ORIGIN      
        1 aaaaagaaaa acccaaaaag ccgtccatga aaccatggga gactcatcag gtttagcttg
       61 catcaggcaa tcactatcca agttgtatcg ttttctttat attatcgaaa gcaaatgctt
      121 ttgtcaacgc tttcttagtg gctattaggc acttctgact agctgtcgaa tcttttttta
      181 aatctggtga ctacgacact aactctcaca gatgaaaaat atgagaagct atgttttatt
      241 ttcactctag tgcttaggtc aaaattctac tgtcacaaca tcttttatat accactgctt
      301 tgcagtttgt acatatctgc atacaaacca ttttgcgcta gaagtgtttc atgattcccg
      361 cgttctacta tatgaccttt gtctaaaact aaaatcaatt ctgcatctcg aatcgttgac
      421 agccgatggg caatcgcaat cgttgtccgt ccttgacgca tttttgccaa tccttcttgg
      481 atcaatcctt ctgtttcagt atcgatattt gctgttgctt catccaatac tagaatttta
      541 ggatcagtca cgattgttcg cgcaaaagtg atcagctgtc gttgtccact agaatagctg
      601 gcaccatgtt cgatcacttt tgagtgatat ccttttggta attcatcgat gaatttgttt
      661 gcttgaacaa actcagccgc atggcgtatc tgttcgtctg tgatagctgg attcatcatc
      721 cgaatatttc ctgcgatatc cccataaaac aagaaagcat cttgcaagac cagccccatt
      781 ttcttacgta actcttccat tggatattct cgaatgtcat gatcatcaat caatatctga
      841 ccttcataaa attcgtaaaa ccgcatcaat acgttgatga tcgagctttt tccgcttccg
      901 gtgtgtccca ctaatgcaac ggtctctccc ggattagcaa caaaagagat attgtttaat
      961 acattgtgct ctccatcata agaaaagctt acattgcgaa actcaatctt acctgaagta
     1021 atcatttgat ctgctccagg gttttgttgt ggtgcatatt cttccgtatc cataatcttc
     1081 aagatacgac taccagcaac catcccatct gtgaagatgc tcaaaaagtc catcatttga
     1141 gacattggat taaaaaatcc ctggacatag gtcgtaaatg catagatcag tccaacttct
     1201 acgggagaat gcaacgcatc gatcccaaac aaagtcaaca ccaacgcaat cgccaatgca
     1261 tatagaaaat tgatgatcgg acttaacaga atcgaattcg ttttgatcat cgcataacgc
     1321 gtgtctaaat actcattgtt cacttgttca aattctttag ctaaacgagc ttcttggcga
     1381 aactgctgga taatacgcat gccggaaatc gattcattca atttcgtgtt cagctgactt
     1441 aatttttccc gcatatttcg gtaaatacga gaactgaatt tctggtaata ccagatgacg
     1501 attcctaaaa tcggcaaaaa caccaaagtc cacaaggcaa tttctccatt gatttgaaac
     1561 atggcaagga atgaagaaac gactgcaaag atcccagtca ataccatcaa aaagacgtac
     1621 caaaattcaa atagcgtttc tgtgtcattc gtcactcgag aaacggttga tcctgccggt
     1681 gtctgatcaa aataacgcat acctagtgta tgcagctttt caaataattt taccctaata
     1741 tactgatagg tcttcaatga ggccatggaa tacaaatacc attggaaaaa ccaaatgata
     1801 ctttttacta tgactccaaa taagtacata ccagcaaaaa acaaaataac ttgtgtcgta
     1861 gccgactgtt tagccagatg atcatccata aaagtttgga tgatccttgg caataggaca
     1921 ttgatcacag acaaagcaaa cgcaaataag atagcaacga tgaacgttct tctaaaaggt
     1981 ttggcgaact tcatcagtcg tttgatgatc tttttctgtt ctttaagtgg aatcgatttt
     2041 gtccattccg attgataatt ttcatccatc aggcctcgcc tccttctatc ttcgcttcta
     2101 attgctgttt ctcccacatt tgttcatacc agccgcctag tgcaagcagc tcatgatggg
     2161 ttcctcgttc aatgattttc ccttgatcga caacaatgat ttcttttgca tgcatcacgc
     2221 tacttaaacg gtgagctgca atgatagttg ttttctcttt tcttatttct tttaaattat
     2281 gcaagatagc ttcttctgtt ttggcatcta ctgcagagag tgcatcatct aatattaata
     2341 attcaggatt tttgatcaat gctcgagcaa tcgaaatcct ttgtttctgt ccgccagata
     2401 aagagactcc acgttctcca actaaggtat cataaccttt tggtaattcc ttgatttcat
     2461 tgttgataaa tgctaaagaa gccgcatgct ctacttcttc ttctgaaagt tttggattag
     2521 aaaaacggat attatctcga atggtcatcg aaaacaagaa gtgatcctga ggcacatagc
     2581 cgattgatcc taataaagca ttcaatgaat attctttgat cgaacgtccg ccaaattgga
     2641 ttttcccttg ataatcatcg tattcacgca tcagtaattt gatgatcgtc gtttttcccg
     2701 caccagtttt cccaacaata cctaatgttt ctccttcttg gatcgcaaac ttgatttgtt
     2761 ctaatgctgg tgattcttct ttcggataat taaaagagga aatagctaca ttcagctcgc
     2821 cttttgccgg ggtctgcact gcacccagtt gttccacgat atgcgtcttt tcatgcaaaa
     2881 gttcattgac gcgatcataa gaagcatttc ctctttccaa tacgttgaac aatcgaccaa
     2941 tcgcaaacat cggccaaaca agcataccga tataactaat aaatgagacc atctgcccga
     3001 tcgagatcgt gccttccaag acatactttc cacctaaaat aattgttgca acataagaca
     3061 gaccgataat caaggtaatg aatggatcaa ataaggagtc taaaaaattg actcgtttgt
     3121 ttttaataat tgcatcttca atcttttctt caaaatccgc tagatcttct ttttcttgtc
     3181 caaatgtttt aatgactttg atcccagtga tactttcttg tgtcttatca ttgatactag
     3241 aaaaagcttc ttgcgaatca cgaaaagcat catggagttt tgaccccagc actcgtgaag
     3301 tgacggccag caaaggcaga ggaatcagcg caatcagtgt taagcgccaa tcaataaaca
     3361 aaatcatcgc aatgatcgtt gttccgcccg taatcactga atctgcaaag gtcaagatac
     3421 cagctcctgc tacattctgg atcgcattca aatcattcgt tgcgtgagcc atcaaatccc
     3481 cagtccgata tttttggtaa aagagattgt ccatttctgt aaaatgtttg aataactgct
     3541 gccgaagttc tttctccaat cgagctgcac ttccccaaat attcgtccgc cagatataac
     3601 gaaaaagata ttgtaaaaaa gcagcgataa tcaataggaa gatccacccg aaaatgatct
     3661 ttattgataa atttttatcg gcgatctcat caatgatgat cccaatcact tttggaggaa
     3721 ctaactgtac caaggccact gcgaatagcg agaaaacccc aattaaataa tgtttctttt
     3781 cctgacgaaa aaaccagcct aattttctaa aaatcgacat tttactcctc ctttgtcttt
     3841 atcaagagcc taggattgtc tgtgcttgtc ttttttgctg aataatctga atagtctaac
     3901 aaattacgaa ataaaaaacg gactgaaaaa atcagcccgt aaaaagtcgt tgggtcagaa
     3961 cctatttttt cccttggttc ttcatttgct gcatcatctg gcgaattttc ttctcagatg
     4021 gtttttgccc cattgacatc atcattgatc gcaacatgtc ctcattaacg ggaggatttt
     4081 tttctaaata gtctttcata tatttacgcg ctaggaaaaa gccacctact gcgccgatta
     4141 gcaaagcaat aatagcaata agtacgacaa tacctgttga taacatataa atctctcctt
     4201 tctatttccg aattgctctc aatgacttat tttactaaag tttattgaaa aaagaaaggc
     4261 gtattcttta gtttcatgca aaaaatagtg gtttttttag aggagaacaa aatttctttc
     4321 gtcatctagc aagacgcgcc atgaaaaaaa attaggcgta ccgaatcttt ttctttaatt
     4381 ttttcttttt gaggtgtaga atgtaatcgt agataaatag gaaaggtggt tttttcttga
     4441 ataatcaatc caataaacaa tttcataagc atcgacttat tgaacataca aatggtttat
     4501 ctttagctga agtaaatgga acagttgatg taccaaaggg gaaaagtttt tttaaaacac
     4561 ttcttgctta ctctggacct ggtgcgttag tagcagttgg gtatatggac ccgggtaact
     4621 ggtctacttc gatcaccgga ggacaaaatt tccaatattt attgatgtca gttattttga
     4681 tgtctagtct gattgctatg cttttacagt atatggcagc aaaattagga atcgtgagcc
     4741 aaatggattt ggcacaggca atccgagcac gcacaagtaa gacattaggt gtcattttat
     4801 ggatcttgac tgaattagcc attatggcaa cagatattgc agaagtcatt ggtgcagcaa
     4861 tcgccctcta cttacttttt gatattccat tggtgattgc cgtctttatt actgtattag
     4921 atgttttcgt tctcttacta ctgacaaaag tcgggtttcg taagatcgaa gcactggttg
     4981 tctgcttaat tttcgtcatt ttattcgttt ttgtctatca agttggcttg tctaacccag
     5041 attggcacag tttatttgct ggctttatcc ctaacagtca tacattctca gactcaccgg
     5101 tcatcggcgg cgaaactccg ctgactggag ctttagggat tattggtgca acagtcatgc
     5161 cgcataactt atatcttcat tcagcagttt cacaaactcg gaagatcgat caccaaaacg
     5221 aagaagatgt agcacaagct gtacgttttt ctacttggga ttctaatatc cagttgtcaa
     5281 tggcttttct agtcaatgca ctgctattga tcatgggtgt cgctgtcttc aaaaccggtt
     5341 ctgtccaaga tccttccttt tttggtctgt atgaagcttt atctgatcca tcagctatga
     5401 gcaatggtat tttgattgct gtagcaaaat ctggtatctt gtccacttta tttgctgtag
     5461 cacttcttgc ttctggccag aactctacta ttactggtac gttgaccggt caagtcatca
     5521 tggaaggatt catccatatg cgtatcccga tttggctaag aagactggtc acacgattgc
     5581 tttctgtgat tcctgtattg atttgtgttt tgtttacaag tacaaaagga acgatcgaag
     5641 aacatgccgc tttgaataac ttaatgaatg aatcccaggt gttcttggct tttgcgttgc
     5701 cgttttcgat gattccttta ctgatgatga ccaacagcca agcggagatg ggtagccgat
     5761 ttaaaaattc attcatcgta aaacttcttg gctggttctc tgtgattagt ctaacttact
     5821 tgaacttacg aggattacca ggacagatcg aaagtttctt cggcgagcaa gccacttccg
     5881 ctcaactagc attggctgat cacatcgctt atgtaatcat tgttcttgtc cttctcctgt
     5941 tggcatggac gattggtgat ttatataaag gaaacaaaaa atatgaacag cttttgcagg
     6001 aaacggtaga agaaatcgaa taaaaactga tttttccaca caaagaccct tgatagttag
     6061 gttaaaaatc tagctaccaa gggtcttttt atttagaaga taaagctttt ttcagattta
     6121 atttactgaa aactagcatg cttgttacta cgatgaattg cgttaatggc acgcctgcac
     6181 cagtgtagac acttaaaaaa tagtcctttt tttctggcgc ataagaacaa aaccatgaga
     6241 caccgaaaag aaaaaccaat tgtgtcagta ttaatccggt gaccacaatc aaagaggaac
     6301 tataataggc acgatcagct tttgcaaata cggttcctag tatcataagt aatatccctg
     6361 ctattgaagc aatcagcacg ggaatagcca gaacttggta aatttttatg atgacattcg
     6421 cgacttttgc tgattttgtt aattggaaat taggtgttct cggttcatta ctatcgatcc
     6481 aattctccat tagcggaagg tttaataatt cttgagcctg tttgacttgt tcttgcgttc
     6541 cgaaactata atttcctcct tgatggaaat ctttatatcg ccatgcataa gcgtatcctg
     6601 attgaaggaa atcgaatacg attggcagat cactcatcgt ctttccatct cctgatccca
     6661 tcagatagaa ctcttttttc tttttgattg ttcccttgtg ataggcttct tctaattctt
     6721 ggttgacttt tttccaaaaa tcattggttt cttttgcatt gttttggtaa taaccagatt
     6781 cgcttaccac atctcgcaat gcccaaaaga tgatatctcc gggtaattct tttacttttc
     6841 caccttgagt ccacgcgtga gtcgtatata attcgtcaat acgggtagaa acagtatgga
     6901 atgtaggaga tgcggctaat gctttattta gtgcagcatg tgagacccat atctcactgt
     6961 tttctttatt caagttcgtt tcatcatcta ttcggatcag ttgtttcatc aaacgtccaa
     7021 attctccact ggaacggtca ttgatggttg caataccgta tgttgtctca tttttatatt
     7081 cgatccattg atagacgatg gaaaagccaa ctataggaag taaaaacaat gaaagtttca
     7141 gccattcccg tttcgagaga agaaagagtc gtttttttaa cttctttact tctttgaaag
     7201 aaaagtcttg ccctagcaag atctggacga aactgatacc caaagctact acagtaaaag
     7261 gaagaagcca gatagaatct tcgcgaatga accaaaagaa aggaaaagcg acagaaagcc
     7321 ctaaaaacca tggcagcaat tttctgctag aaccgtattt gcggtaataa atagctaata
     7381 aacaagcgat aatgatctca acagccggaa tcacgagggc attacgatat gttcgagtgg
     7441 aatattcatg tgtaaaacta atcggagaaa acaaaaaata aaaatagata cttaaaagaa
     7501 gatacttgtt cttcacaatc ggtttaaacg cgagtgcaac caatatactg gcaaaaatac
     7561 tgaacatgcc gaacaaaact gcataagaaa gatgcagttt atgtgcaagt actaaaaata
     7621 agctgtagga gattcctttg gaaagtgttg tagtcgtata ctcccctagc catttaccgt
     7681 ccaaaatatt ttgagcatag cgaatcagca gctggtcatc ataggttgcc gacatgtctg
     7741 ttccatagat tgctttacta tataaataca aacgaataat tgtaaataat agggctatgc
     7801 caagaataaa gtatgtatgt ttctttttta gttggttgat caagggtttt aaagtgttca
     7861 gcatcttttc gtccttccaa tcacgttttg ccacttgctt gtgaaacttt tcgttttttt
     7921 acttttctac cagtatacct tgtttgtcta aatttaaaaa gaggggggag aagatgcatg
     7981 atgaaaaaga ctgcaaaaag tactcctttg tttcttaaac aaaaaaggac aaggcaatgt
     8041 gcctggtcct tttttgctgc tgtcagatag aaaatcttag agtatcactt cttctgctaa
     8101 taactcagga aacacttctg gatcgaatgt ttgcgctttg aacatggcga tttcttttcc
     8161 atatggtgcg taagctttct ttttatcttt gcctacatag ggagtttcca agattttcga
     8221 aatattgtca aacattggat catggacgat cttattcagc gtgtcgaatc cgattgttcc
     8281 aaaaccaata ttggcgtgcc ggtctttatg cgaaccttgc ggatttttag aatcattgat
     8341 atggagtact ttaagccgat ctaacccgat gatcttatca aactcttcca ttacacctga
     8401 aaagtcttct ttaatattat aaccggcatc attcgtatga caggtgtcaa aagtaacgga
     8461 taatttttca ttcaatttta ctccatcgat gatttgcgct aattcctcaa atgttcgacc
     8521 gatttccgta cctttacctg ccattgtctc caaagcgatt tgtgccgtct gttcttttgt
     8581 caaaacttca ttcaagcctt caataattct agcaattccg gcttctgttc ctgcccctac
     8641 atgagcacct gggtgaagtg taatttgagt agcgcctaaa gcttccgcac gaacaatctc
     8701 ttctctcaga aattggacgg caaaaggaaa catttctggt ttgatcgtat tccccaaatt
     8761 aatgatataa ggggcatgaa cgacgatatt tgttagacct ttttctgcca tgtacgcttt
     8821 tccggcatca atattcatct cttcgatggc ttttcttcta gtgttttgag gggcgcctgt
     8881 atagatcata aaagtcgaag atccataact ggctgcttct tctgctgctc ctaacagcat
     8941 tttctttccg ctcatgctga cgtgtgagcc taataacatg gatggattcc tccttgaaag
     9001 gcaattgtta ctaacaattc ctatatattg gtaagatgat atacaaagaa aatgattgat
     9061 ttttctttgc cctgttctcc atagtaactg aaattttctc taaagaaaag cttaagaact
     9121 cttttataat aaagatcatt ttctcacgtt ttaactcttt taaaaaagaa attcctgcca
     9181 aaaacagcct tgtctgtttt tttgttctat tatttgctat aattagcgaa ataaaaccat
     9241 tagttgaggt gacccttttg ccacgcaaaa atactcgaaa aaaacagaat agaagaaaaa
     9301 aagaaaaatg gtttgttcct aagattattt ttagagtttt tcaatcattg actgttttta
     9361 ttactgttct tcttttgatg ttcgcagctc taggtattgg aataggcgct ggctattttg
     9421 cctatctcgt cgaagacaca caattgccga ctaaaaaagc actgcagaca gaactaggaa
     9481 acattacaga aacgtcaaag attgtttatg cagataatac agagatatca aaaatccaga
     9541 cagatctgat gcgcacgact atttcatctg ataagatttc acctttattg aaaactgcaa
     9601 ttatcagcac agaggatgaa tacttcgata aacaccaagg ttacgttcct aaagctgttt
     9661 tacgagcatt agtatctgaa gctactggta ttggctcttc tggtggatca actttgacac
     9721 agcaattagt caaacagcag atccttacag atgaaacaac attcaaacgt aaagcaaacg
     9781 agatcattct ttctgcacaa gtagaaaaat acttttcaaa agatgagatc attgctacgt
     9841 atttgaatgt ctcaccattt ggtcgtaata ataagggaca aaacattgct ggagtccagg
     9901 aagctgcaaa aggaatcttt ggtgtagatg caaaagatgt gacgctgccg caagctgctt
     9961 atatcgctgg attaccccaa agcccgatca cgtatagtcc atacacgaac acaggcgcgc
    10021 taaaagaaga tctttctgcc ggccttgcca gaaaagattt tgttttattc agtatgtatc
    10081 gtgaaggcca aattacgaaa gaacaatacg aagaagcaaa agcctatgac cttacaaaag
    10141 actttctacc acaacaaatc gcagaacaga ctgacagaga attcttgtat tatactgtga
    10201 tgaatgaagc aacaagaatc attgcacaac aattggctga aaaagacaac gctgatatgt
    10261 ctgattcatc ggtcagtgac gcttattatc aaaaggcaca gcaaacgatc caaaataaag
    10321 gatatacaat ccattcgaca atcgataaag atgtctatgc tgcattgcaa aatggcgttg
    10381 aaaattatgg atacatgtta gatgatggtg ctggcagtca agtagaaacc ggaaatgtat
    10441 tgatggataa cagaactggc cgcatctacg gattcgttgg cggacggaat tacagtcaga
    10501 atcaaaataa ccatgccttc gatacagaac gtcaagctgg ctcatcaatc aaaccagttc
    10561 ttgtttatgg acctgcaatc gacatggggc ttatcggatc agaatcccgt gtatcagatt
    10621 atgcaacaac ttggcaagaa ggagaaaatg ctggcgagaa aatcgtcaat gctactaacg
    10681 aaggatcgaa caccttccaa accgtccgtg agtcactaga atggtccaac aatatcccgg
    10741 cttatcactt atatcaagat gtgttgaatt ccggtggatc gaaacaatat gcttacgagc
    10801 actacttggc aaagatgaac tacccggcaa atgacaattg gggcgttgaa tctgctcctt
    10861 taggtacagt agatgtcacg acgcttcaac agaccaacgg atttcaggct ttagcaaacg
    10921 gcggcgtcta tgaagaaggc tatatcatcg attccatcac agataacgca ggaaatgtaa
    10981 tttataaaca cgaaagcaac tctgtccgga tctattcgga agcaacggca tcgatcatga
    11041 atgacatgat gcgtagtgtg atcaatgcaa aaatcactac accattcaaa gatgccatct
    11101 caagcttaaa cggaaatcta ggaaaagcag attgggtcgg aaaaactggt tctaccaatg
    11161 aataccgaga ttcatggcta attgtctcta ctccgtctat taccatcagt agctgggcag
    11221 ggcacgatga caatactgga atggacagca aagcaaggat acgttcggct aattatcttg
    11281 ccaatctgat caatcaagct taccaagcaa aacctgacat ttttggcact gatgagaaat
    11341 tcgaattatc atctgatgtg atgaagaaaa aagtatctgg atttactggt caaacacctg
    11401 gaaaagttac tgtcaataaa aaatccatcc aaacaccagg aaaaacagtt gaatcgctat
    11461 gggcgaaaaa tggaccgaag aaaagcgcct ttaaatttgg cgttggcggt accgatgaaa
    11521 actatacaga ttactggaac accgcttcag cttatgcaag agcgaatcaa gcaactaaag
    11581 aatcagatga gaaagatgat tgatttaata gaatacacag taatggctct tcgtcaatga
    11641 gtgttggtga agaaattcaa tagaataatc gagatactca cacggctaga cagctatctt
    11701 gtctagccgt gttgttgttc tggctttagt cagttgggta gtgttcttag ttttcttgaa
    11761 atattttcct cgatataaaa agattcgttc tcggtagttt tgaaagtttc taaagccata
    11821 ggcaattcgt tttagtactt tgatatggtt gttcaaacat tctatagggc cgttggaata
    11881 gggaactcgc aacgctcgtt tgatttctgt tcgatatttc ttgaaggtcg tgaacactgg
    11941 ttgatagtgc tctggcaact ccttgtagtc ttcttttagg agcgcgacga attcaggaac
    12001 atcttgtctt ctgatagcgg aaagaaaaac ctggtagact tcgtagcctc gctttaagga
    12061 gtcatcataa tataatagcc gatcgactat ctccgcttct gtcagatgat ctcgaaatga
    12121 cggacgccag tagcgatgct gaccattcaa ttcccacgca ttcttttgaa gtagtctcca
    12181 gtatttcttt aagtgcttcg accgacgatc ccctttttta aaggtcttca ttacttgaat
    12241 tcggtggttg gtaaaggccc gaccgatgtg ttggctaata tgaaagcgat cggcgacgat
    12301 tcgggcattc ggaaaacact ctttcaccaa tgtgcgatag ggtgtataga aatctgaaac
    12361 cactagacgt acttgttcac ggacttttct agggtagcgc aagaaatagt cgcgtaaagg
    12421 attgagttgc cgattttcca caatatcaat cagttgatgg gtcttgccgt ccatcatgac
    12481 aaagctcatg gctcccgaga cgtttttcac cgatttgaat tcatcgaaac acaccacttc
    12541 tggtaacggc tggttcaaag aaacggtttt aggccgaagg ctgcgaagta ttcgaatgac
    12601 agtggtcgga gaaatgtgct tcatacgtgc aatcaaagac atagaaatag gctcacttaa
    12661 tagctcaaga atggcttgtt tgactctgcg ggcaatggag cagcgacgat cgctaactga
    12721 atcttctgct aaaaaagtct ttccacagtg tttacatttg aagcgctgtt tgttaatccg
    12781 cacaatggtc ttgtagttac tgacatcatt caataagatg gatacttttt tccagcccca
    12841 tttaatgatc gtttgcttat tttgatagtg gcaatggaag cattcctctg gttgataggt
    12901 caatgtgcca tcaaaaacaa aacatgtttc gtcattaatg cgctctttgc gaaaggcatc
    12961 ttcatgaaag gtaagattta ggtctagaat atctagtaac tctttggtaa actctgaata
    13021 ggacatcttg aaacactcct ttgaatggtt ttcgtcgact ttattctaaa ggattt
//
LOCUS       DACBZN010000077        13073 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.077, whole genome shotgun sequence.
ACCESSION   DACBZN010000077 DACBZN010000000
VERSION     DACBZN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.077"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..1424)
                     /gene="tkt"
                     /locus_tag="IVD64_002030"
     CDS             complement(<1..1424)
                     /gene="tkt"
                     /locus_tag="IVD64_002030"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP6149271.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEP"
     gene            complement(1526..1777)
                     /locus_tag="IVD64_002031"
     CDS             complement(1526..1777)
                     /locus_tag="IVD64_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP6149272.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            1924..2547
                     /gene="lexA"
                     /locus_tag="IVD64_002032"
     CDS             1924..2547
                     /gene="lexA"
                     /locus_tag="IVD64_002032"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP6149273.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            2727..2945
                     /locus_tag="IVD64_002033"
     CDS             2727..2945
                     /locus_tag="IVD64_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149274.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3234..3746)
                     /locus_tag="IVD64_002034"
     CDS             complement(3234..3746)
                     /locus_tag="IVD64_002034"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP6149275.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(3760..6057)
                     /gene="recJ"
                     /locus_tag="IVD64_002035"
     CDS             complement(3760..6057)
                     /gene="recJ"
                     /locus_tag="IVD64_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP6149276.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6199..6633)
                     /locus_tag="IVD64_002036"
     CDS             complement(6199..6633)
                     /locus_tag="IVD64_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP6149277.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(6643..7431)
                     /locus_tag="IVD64_002037"
     CDS             complement(6643..7431)
                     /locus_tag="IVD64_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6149278.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(7428..8369)
                     /locus_tag="IVD64_002038"
     CDS             complement(7428..8369)
                     /locus_tag="IVD64_002038"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP6149279.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(8546..9133)
                     /locus_tag="IVD64_002039"
     CDS             complement(8546..9133)
                     /locus_tag="IVD64_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP6149280.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(9354..10667)
                     /gene="obgE"
                     /locus_tag="IVD64_002040"
     CDS             complement(9354..10667)
                     /gene="obgE"
                     /locus_tag="IVD64_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP6149281.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            10955..11965
                     /gene="gap"
                     /locus_tag="IVD64_002041"
     CDS             10955..11965
                     /gene="gap"
                     /locus_tag="IVD64_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6149282.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12050..12985)
                     /locus_tag="IVD64_002042"
     CDS             complement(12050..12985)
                     /locus_tag="IVD64_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6149283.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3595 a         2933 c         2307 g         4238 t
ORIGIN      
        1 ggttcgtgcg ttggtccgtc ttctccgaca gcaaccgagt catgtgttaa gacgtaaaca
       61 acaggagtat gttggattgc tgctaaacga actgctggtc ttagataatc gacaaagaca
      121 aagaatgttc caccgtagat acgagttccg ccatgaagtt ggataccatt catagccgaa
      181 gccattgcaa attcgcggac accgaaccag atgttgcgtc cttcataatg ttcaggagtg
      241 aaatctttat ctgcagccac cattgtatta ttggaacctg acaagtccgc tgaaccaccc
      301 cagaagcttg gtatagcttt cgataattct tggatgactt ctttacttga aacacggctt
      361 gcttgactgc ttccaacttc ataagttggc aattctgcat cccagttttc tggtagtttg
      421 ccatcaaaag catcttcaaa ttgttgtgct aattcaggat aagctttttt ataattggca
      481 aacatttcat tccatgcatc ttcagctttt tgtccttctt ctatcatcgt ttgatggaag
      541 cgggcagcta cttcttctgg aacagtgaaa tctggatact cccaaccata aacttcttta
      601 gccatcttga tgccatcttc accaagagga gcaccatgaa cagcagatgt tccttcttta
      661 ggagcaccat agccgatgac tgtttttact tcgatcaatg ttggtttgtc tgtttcagct
      721 tttgcttctt caatcgcttt agagattgct tctaggtcat ttccgtcttt cactaagata
      781 tgttgccagc catatgcttc atagcgagca ccaacgtttt ctgtgaaagc ttttgaagtt
      841 ggaccgtcta gtgaaatatc gtttgaatcg tataaaacga tcaatttccc tagtttcata
      901 tgacctgcca tcgaacttgc ttcttgagat accccttcca tcaagtctcc gtcaccgcat
      961 attgcatacg tatagtgatc catcacatta aagttttctt tgttatacgt tgctgcaagg
     1021 tgcgcttctg ccattgccat accgacagcc attgcaatcc cttgtcctaa tggccctgta
     1081 gtggcttcta caccatctgt atgatgtact tcaggatgtc ctggtgtttt agaatcccat
     1141 tgacggaatt gtttcaaatc atcgattgtc acttgatagc cagctaaatg aagcaagcta
     1201 tacaacattg ctgacccatg tcctgccgaa agaacgaaac ggtcgcggtc agcccagttt
     1261 tttgatgtcg ttggatttac tttcaggtgt tttgtccata acgcatatgc cataggtgct
     1321 gcgcccatcg gcaatccagg atgtcctgag tttgcttttt ggactgcttc aatacttaat
     1381 gtacgaattg tgttaacgcc aagttggtca attttgtcaa acaaaataac ccctcctatt
     1441 aatttaggtt ttttttacct ttatttctta cactttcatt ctataagatg tcacacgaaa
     1501 aagcaatctt tttcagtatt ttttactaaa agttgtcatt tcggttgtgt aagccttttt
     1561 tcttttggat ttcttttaat ttttccgggg tcacgtcatt gccctcagga tcgacgactt
     1621 tcattccttc gatatgatga cgcataccgg aacgaaaagc ttccaaatac tctttgcgca
     1681 agttgatttg ttcttgttct tcggcttttg agagaccatt ttccttcttt ttacgtgcaa
     1741 gttcattgat ccgctctatt ttttcaggtg acagcatgct cagccctcct tttactctcc
     1801 ttcatattat ataaaaactg aaaaaaaaac aactagataa actaaataaa atacgaacat
     1861 tcgtttgatt tagcgctttt tatatggtac tatttattta tcaagtgaaa gaaggtgtac
     1921 cttgtggccc gacaaactga atcaagacaa atcgaagtct tacgatatat ccatgaacaa
     1981 gtaacccaaa aaggataccc gccgactgtc cgtgaaatcg gggaagctgt tcagctttct
     2041 tctacttcta ctgttcacgg tcatcttgca cggcttgaaa aaaaagggtt tatccaaaga
     2101 gatccgacga aacctcgtgc aatcgagttg acaaaacaag gtttagaaaa gattgggatt
     2161 cgtccaacaa ctattccagt acttggcgtc gtaacagctg gagaacccat tttagcagta
     2221 gaggatgctt cagacttctt tccaatccct cctaatcttt cttccgaaga aggcagtctc
     2281 tttatgctga caattcgtgg agatagtatg atcaatgctg ggatattgga tggtgacaac
     2341 gtgatcgttc ggaagcaaga aactgctcaa aatggcgata ttgtcattgc aatgactgcc
     2401 gaggacgaag cgacttgcaa acgattctat aaagaaaaaa actttttccg tctgcagcct
     2461 gaaaacgact tgctcgaacc aatcatctta gatcaagtat ctatattagg acgagtagta
     2521 ggactttatc gtagtcatat ctattaattg ctactaatta aaagaaaact ttttaaaaag
     2581 agtttgagac gttggtctca aactcttttt tcgcccgggt tcttcttctt tcaaattttt
     2641 atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt
     2701 cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa
     2761 ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt
     2821 ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg
     2881 agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat
     2941 cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg
     3001 tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg
     3061 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
     3121 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
     3181 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
     3241 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
     3301 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
     3361 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
     3421 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
     3481 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
     3541 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
     3601 attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca
     3661 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     3721 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     3781 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg
     3841 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     3901 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     3961 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     4021 ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     4081 caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg
     4141 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg
     4201 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     4261 ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc
     4321 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     4381 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     4441 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     4501 accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg
     4561 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     4621 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     4681 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     4741 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     4801 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     4861 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     4921 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     4981 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     5041 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     5101 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     5161 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     5221 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     5281 cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat
     5341 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     5401 aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     5461 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     5521 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     5581 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     5641 gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa
     5701 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     5761 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     5821 gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc
     5881 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     5941 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     6001 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     6061 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     6121 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     6181 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     6241 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     6301 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     6361 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     6421 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     6481 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     6541 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     6601 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     6661 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     6721 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     6781 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     6841 atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac
     6901 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     6961 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat
     7021 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     7081 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     7141 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     7201 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     7261 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     7321 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     7381 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     7441 tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac
     7501 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     7561 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     7621 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     7681 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     7741 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     7801 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     7861 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     7921 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     7981 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     8041 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     8101 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     8161 aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata
     8221 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     8281 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     8341 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     8401 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     8461 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     8521 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     8581 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     8641 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc
     8701 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     8761 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     8821 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     8881 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     8941 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
     9001 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
     9061 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
     9121 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
     9181 tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
     9241 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
     9301 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
     9361 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
     9421 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
     9481 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
     9541 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
     9601 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
     9661 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
     9721 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
     9781 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
     9841 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
     9901 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
     9961 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
    10021 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt
    10081 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
    10141 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
    10201 tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga
    10261 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
    10321 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
    10381 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
    10441 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
    10501 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
    10561 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
    10621 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
    10681 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
    10741 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
    10801 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
    10861 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
    10921 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
    10981 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
    11041 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
    11101 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
    11161 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
    11221 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
    11281 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
    11341 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
    11401 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
    11461 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
    11521 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
    11581 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
    11641 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
    11701 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
    11761 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
    11821 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
    11881 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
    11941 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
    12001 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
    12061 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
    12121 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
    12181 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
    12241 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
    12301 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
    12361 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
    12421 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
    12481 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
    12541 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
    12601 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    12661 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    12721 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    12781 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    12841 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    12901 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    12961 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    13021 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DACBZN010000078        13052 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.078, whole genome shotgun sequence.
ACCESSION   DACBZN010000078 DACBZN010000000
VERSION     DACBZN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.078"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(77..149)
                     /locus_tag="IVD64_002043"
     tRNA            complement(77..149)
                     /locus_tag="IVD64_002043"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IVD64_002044"
     CDS             530..1099
                     /locus_tag="IVD64_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP6149284.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IVD64_002045"
     CDS             1086..1946
                     /locus_tag="IVD64_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6149285.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IVD64_002046"
     CDS             2136..2840
                     /locus_tag="IVD64_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149286.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IVD64_002047"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IVD64_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP6149287.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IVD64_002048"
     CDS             4677..5993
                     /locus_tag="IVD64_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149288.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IVD64_002049"
     CDS             5994..6863
                     /locus_tag="IVD64_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149289.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IVD64_002050"
     CDS             6918..7727
                     /locus_tag="IVD64_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6149290.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IVD64_002051"
     CDS             complement(7830..8459)
                     /locus_tag="IVD64_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP6149291.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IVD64_002052"
     CDS             8629..9921
                     /locus_tag="IVD64_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6149292.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IVD64_002053"
     CDS             complement(9997..10659)
                     /locus_tag="IVD64_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6149293.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IVD64_002054"
     CDS             10884..11168
                     /locus_tag="IVD64_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP6149294.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IVD64_002055"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IVD64_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP6149295.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4339 a         2255 c         2824 g         3634 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt ac
//
LOCUS       DACBZN010000079        13003 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.079, whole genome shotgun sequence.
ACCESSION   DACBZN010000079 DACBZN010000000
VERSION     DACBZN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.079"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(22..1806)
                     /gene="pyk"
                     /locus_tag="IVD64_002056"
     CDS             complement(22..1806)
                     /gene="pyk"
                     /locus_tag="IVD64_002056"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP6149296.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(1880..2842)
                     /gene="pfkA"
                     /locus_tag="IVD64_002057"
     CDS             complement(1880..2842)
                     /gene="pfkA"
                     /locus_tag="IVD64_002057"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP6149297.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(3010..6324)
                     /gene="dnaE"
                     /locus_tag="IVD64_002058"
     CDS             complement(3010..6324)
                     /gene="dnaE"
                     /locus_tag="IVD64_002058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP6149298.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            6555..6740
                     /locus_tag="IVD64_002059"
     CDS             6555..6740
                     /locus_tag="IVD64_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP6149299.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(6802..7998)
                     /locus_tag="IVD64_002060"
     CDS             complement(6802..7998)
                     /locus_tag="IVD64_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6149300.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(8168..9604)
                     /locus_tag="IVD64_002061"
     CDS             complement(8168..9604)
                     /locus_tag="IVD64_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6149301.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            9897..10706
                     /locus_tag="IVD64_002062"
     CDS             9897..10706
                     /locus_tag="IVD64_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6149302.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(10892..12889)
                     /locus_tag="IVD64_002063"
     CDS             complement(10892..12889)
                     /locus_tag="IVD64_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP6149303.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         3782 a         2755 c         2328 g         4138 t
ORIGIN      
        1 tttatttttt attaagctaa attagatagc tgttgtttcg ccacggtaaa cgatgccgcg
       61 acgtgaatca acggtaatca attcaccttc tgtaattgtt gttgttgcgc ctttagcacc
      121 aacgattaca ggaatatctt gtgcaattcc tacaactgca gcgtgagaag tcaatccgcc
      181 ttcttcaacg ataacagcag ctgctttttc gattgcaggc atatattctt tgtcagttga
      241 tggtacaact agaaccattc cgtcttttgc tttttttaca gcttcttcag cagatgaagc
      301 aataactgca tttgcaacga cagatttttg tccaacgcct tgagcttcta ataatttaga
      361 accaatcatt tgaattttca tcacgttagt tgttccgcgt tcaccaactg gtacaccagc
      421 agtgatcaag attaaatcgc cttctgatgc gaaacctaat tcaacagctt ttttagctgc
      481 tagttcaaac atttcatcag ttgttgatgg tttttcagca actgttgggt aaacgcccca
      541 gtttaacatc aaaccacgtt ttgtgcgttc gtcaaaagta acagccaaga tatctgcatc
      601 tggacgatat tttgaaatca tttttgcagt gtatcctgat tcagttgcag caacgattgt
      661 tttcacgcct agattttttg cagcacgagc tacagataaa ccgattgttt cagttacatc
      721 tgttttatcg aattcgttca attggaatgt acctaattct gataatgcat tttcagcttc
      781 ttggtcgata cgtgccattg ttgaaacagc ttcaactggg tagtccccgt ttgcagattc
      841 accagatagc attgttgcat ctgtgccatc aaatacagcg ttagcaacgt cagaagcttc
      901 cgcacgtgtt ggacgtgggt tttcttgcat tgattctaac atttgtgtag cagtgataac
      961 tgggatacca gcagcattac attttttgat aatgcgtttt tgaaccattg gtactagttc
     1021 agctgggatt tcaacaccca tatctccgcg ggcaatcata acaccgtcag aaactttgat
     1081 gatttcgtca atgttgtcga taccttcttg agattcgatt ttagggaaga tttgaacatg
     1141 agtcatgtct ttttcttcta agatttcgcg gatatctaga acgtcttgtg ctttacgtac
     1201 aaaacttgca gcgataaagt cgatatcgtt gtctaaacca aaacgaatat cgtcagcatc
     1261 ttttggagta atacctggca aactaataga aacgcctggt gcatttaccc ctttacgtga
     1321 tcctagcata cctgcatttt taacttctac gactaattca cggtttgctt cgtctttttc
     1381 aatgatttgc atatcgatca aaccatcatc aaataaaatg tgtccgccaa cgtgaacatc
     1441 gtcaaataat cctgggtaag taaccgcgat tttttctttt gaacctacat gttcaggatc
     1501 catagagatg cgtgtttgat ctcctacttc aaatttgatg tatcctgcac gaccaaaatc
     1561 agcttctgtt gtgccttgaa cagttgtacg gatttcagct ccttttgtat ctaaaagaat
     1621 accgacatct tttcctgtct ttttaactgc ttcacggacc atagtcatac gtgataattg
     1681 ttcttcatgg tcaccatgag aaaaattaaa gcggaagaca tttgccccag attcaatcaa
     1741 ttgagaaata atttctactg agttacttgc tggtcctaat gtactaacga ttttggtttt
     1801 tttcatttaa aaaactctcc cattcaattt tgtttattta aagctgtaca cagcttaaat
     1861 agcttggttt ctttcactct taaaatgaaa gtgaattatt tagatcatat agtgataagt
     1921 ctggtttatg tttgttgttt tcaagtgtat caatgatgtc agacgcaacg atttcgttgt
     1981 catggatacc gacacataat ccaccttttc cttcttgaag caattcgaca gcgtaagcac
     2041 cgaatttact tgccaacacg cggtcgcgtg cgcttggtgc tccaccacgg acaacgtgtc
     2101 ccaagataga tacacgtgta tgatagtcac caaattctga aagtttctca gcgaattcat
     2161 ttccgcccat tacaccttca gccaagatga tcaagcaatg tttcttcccg cgatcgcggc
     2221 cttcttggat ttttttagca actgaagcca tatcaaaatc atgttctggg atgatgattt
     2281 catccgctcc gccagccaca cctgaccata aagcgatgtc tcctgcattg cgtcccatta
     2341 cttcgataac gaatgtacga acatgagaag tcgctgtgtc acgaatacgg tcaattgatt
     2401 ctagtactgt attgattgct gtatcaaaac cgattgtgaa atcagtccca ggaatatcat
     2461 tatcgattgt tccaggaata cctactgctg ggaaaccgcg ttttgtcaaa gccattgcgc
     2521 catgataaga gccgtctcct ccgataacca ctagaccttc gattccgaat tttttcaatt
     2581 gttcgattcc ttttaattga ccttcttcag ttgcaaattc tggataacgt gcagaataca
     2641 agaatgtacc accacgttgg atcttgtcac ctacatctgc cacatcaaga cgacgaatat
     2701 ctccagcaac taaaccggca aaaccataat tgatcccgta tacttccagt ccttcgtaaa
     2761 tacctttacg aacaacggca cgaacagctg cattcattcc aggtgcgtct ccgccactgg
     2821 tcaaaattcc gatgcgtttc ataataaaca ttcacctcat taaaatactt tatcctaagt
     2881 atgacttagg tttacaacca tttttgcgtt aaccacgact aacattctaa cattattttt
     2941 ctcacttgtc ttgatttacg gataaaaaaa tgaaaaaaaa tgaaagcaag tagtattttt
     3001 taccgtaagt tatttgaaaa ccacattatc ttcgccaagc agataaataa gttgcccctt
     3061 tatactcttg ctgtcatcca cccaataagc atctgacaga actacttttc gttgcgtttt
     3121 ttcgtgatac ataactactg gtatatatcc tgggtgtttc tgaaaaatca cttgtaattg
     3181 tttcattatc tcgggatctt cccgttcttc tgtaatacgc aaatagcaag tttgtgaagg
     3241 atactgggct tctaactggt taggatcagc aaagctttct ccaattagtt gcagttcctg
     3301 attatatttg ctgatttcgc tccgcccttg aacaaagatc actcgatcaa cggcaaacaa
     3361 attacgatat ttacgataaa gagttggaaa aactgtgaca gaaatttctc cactactgtc
     3421 ggttccttca acaaaggcca tcagttctcc tttttttgtc cgaatttctc taacttcctt
     3481 gatcatcact aataaattga ctggttgatt agggacgata tctgtaatca gctgaacttg
     3541 tttagctaaa cgaagacgat caaacccttc tgcaggatga cctgacaaat aaatacctaa
     3601 gtactcctct tccagcttca agcgttcttc tagtgtatag tcagcgactt cttcttcttt
     3661 taatgccatg attcccagca agtcaagact tccaccactg taaacgatat tttggatttt
     3721 tccttcgaga tcaagcatca attgtttcct gttaggagat aattcatcaa aaactcctac
     3781 tgctgtcagt gattgcagta actctaattt cagccaacgc ttatcgatcc gtatgaggaa
     3841 ttgatccact gactggaacg ggccattttg ttttctttcg tttaatatat cttctacaaa
     3901 atcacgccga atacctttta tcgctgtcag tccgaatctg atttcttcct tattgaccaa
     3961 ctcaaaactg gaattacttt gattaatgga cggcgaggaa agtttgagtt tatttttacg
     4021 tgcttctgca atatattctt tcaattttga tgtgttatgc cgaacagaat tcatcaggct
     4081 ggtaaagaaa gcacctggat gatgggcttt tagatacgcc atttggaatc ccacgaatga
     4141 ataagcaaat gcatgtgacc gattgaatcc atagtttgca aatttttcga tatattcata
     4201 tacggtcgct gctgtttttt ccgaatatcc ctgttgtaat gctcctgata caaaatgcga
     4261 cttttcttca tctaatacgt cttttttctt tttactcact gcccgtcgca gaatatctga
     4321 ctgccctaat gtaaatccag ccatagtcga ggcgatctgc atgatttgtt cctgatagac
     4381 gatcactcca tatgtgtttt tcaaaatcgg gatcagtgaa tcatctgggt agcgaatggc
     4441 ttcttttcct ttcttcctgg caatgaaggt atctatattt tgcatagggc ctggtcgata
     4501 caatgcattg actgcggcaa tatcttcgat gttttctgga tctaatcttc gtaaaacgtt
     4561 ccggatacca gcagattcaa attgaaatac tcctgaggtt tcgcctcgtt gaaacaagag
     4621 cagcgttttt gcatcattta gcgggatctc ttttaatcgg atctcatacc cttctactcg
     4681 cttaacagct ttcaaagtaa aatcaataat cgataagttg cgtagtccta aaaagtccat
     4741 tttcaaaaga ccgattgctt ctacctcatt catcgtatac tgggtcagaa aagcttcatc
     4801 agaaccgcgt tggagcggaa ctagttcaag tagattctta tcgctgataa ctacacctgc
     4861 tgcgtgagtc gaaacatgtc ttggcaatcc ttctaaaatc actgcagttt tgaataaaag
     4921 ttggtttttt tcgcttgcct gaactaattc tactaattga tgcgaagttt tataagcttg
     4981 ggtcaatgtg attttcaatt gattgggtat tgcttttgac cagcggtttg cttcactttg
     5041 cgataaacca aacactctag ctgcatctct aagaaccatt tttgctgcca ttgtgccaaa
     5101 tgttgcgatt tgtgccatat gatattgtcc atacttttct cgaacataat tcaatacttc
     5161 ttcacgtcga ttgtcaggaa tatccaaatc gatatctggc atcgtaaagc gttcggggtt
     5221 taaaaagcgt tcgaacagca aatcatattc taatggatcc acatctgtga tttctaacac
     5281 ataggctact aatgaacccg cagcagaacc acgacctgca ccggtaacga ttttgtttct
     5341 atgagcaaaa gccattacat cccatacgat caagaaataa tcatcgaatc ccatttgatg
     5401 gatgacagat aactcgtagt ccaaacgttc ttggtattcc tttgtatagt cagtgattcg
     5461 gcgttccact tgcgaatagc ataactctct taaatattct ccagcttgtt ttccattagg
     5521 aactggataa tgcggaagta atttctgatg gaactggaat tcaaatgaaa tcgattcagc
     5581 taaacgctgg gcgcctatta cagcttggga aagacccgct ttttcgaatc gtttgatcat
     5641 ttcttgttct gaaagaagat agacatttgc tgttagtggt aaagatgcag ataaattttc
     5701 taaaggtatt tggcttccct ctttgatatg tttcatcact tcaagtgcaa aaagttcttc
     5761 ttgttgtaaa ctagcgatgg gatgtaatgc acaaagcgaa taattttctt ttatcacata
     5821 ctgataccat tcaggcaatt gatcaggcgt gttttctgtc aagccgatat aaaaatttct
     5881 agtaaaaaca ttttcaagta aaaactgcca atgaccgaaa aagttatcct ctttattgaa
     5941 agcctcagaa acagaatgat ttgccggaac aacaaacgct agatggtcgg tttctgtcat
     6001 ttctgcaagt tccaattttt cttggatcat ttttcggctg gaaagtttca tcagctgctg
     6061 ataacctatt tcatcttttg cgtacgcaag caagccataa tttttttctt cataagtata
     6121 actaagatgt aatccaatga ttggctgtac tccttcttct acacaaatac gatagaattc
     6181 aggtgcaccg tataagacat ctttatccgt tatggctaat gaagtgtagc cttgtttttt
     6241 tgcttcttgt acatattctc gaatacgtat cgtactttgt aaaagagaat aagaagtaat
     6301 tgtataaagt tgtgggaaaa acatcttgca ttccgccttt tttttcgtta aaattcacta
     6361 gcgttaaaag gtttgatcct tttactttca ttaagatagc aggcgctgaa gttgattatt
     6421 ccacttcatt tgcttgcttg taatctgtag gaatgagttc tttcgcaaga actcaaattt
     6481 tattgagatt tggtctcgtt tctttacatt caaagaaaat ctggtaaaat taagatagaa
     6541 aagaggtgtt ctttatgcga tatatcgtta cattattctg ggctgtcgtt ctaggtcaag
     6601 tagtcggtta tattggtgct gcattaacaa gcggaacata tgattttact ttgacaacaa
     6661 tcatttcatt cattgcgggt gtcattatcc ttttgattgg tgcagttgcg ccaagaaaag
     6721 aaacctctgc ccattcgtaa aaaaacagcc aagagcttga gatttctcaa gctctttttt
     6781 attttctact cctcaccacc attataatct gctgcgaaga actttacgaa agttgaataa
     6841 agaaagaaga atatttgaca gaacaaacaa acttgtcaca ataaaaacag cactgtaatt
     6901 ataagcatgc gcgactgtcg atcctagcat gggacctaaa acttgtccaa agttgctaaa
     6961 catctgattg tagctataga ttcggctgac tccttcttga ggcgtaatct tactgatcag
     7021 tgtattgaca gaaggcatca aagcgccagt tgaaaagcct aaaataaagc gaagtatacc
     7081 taattggagc ggtgtagtga cgaatgccat aggtaaataa cagcaaaatg ataaaatgag
     7141 tcctccaatt agaacttttt gattcccaat cttatcccct agttttccta gtttcggtga
     7201 agaaaagaca gcggaaacac cagctatcga aacaatcaag ccgctgacaa aaaggatatt
     7261 ttctgtactt ccgctcaatt cacgaatata taaggtcaaa atagggctga tcgtagtgat
     7321 tcctatctgt aaaatcaagg tcgtaatgaa gagaccgatc aaaatagaga gatgatccat
     7381 ttttgaaaaa atttctttgg tgcttatcag atcttttttc tcgattggat ggaaatcttc
     7441 tttgactaag aaaatggtca ataccgttgt gatcatcaac aaagcccctg tgatcaagaa
     7501 tacattttcc ataccaaacc actgagccag tgcaccgccc attgaaggtc cgatcagatt
     7561 tccagcaatc gctccggtag acaatgttcc taacgcccat ccgctctttt ctctaggtgc
     7621 ctgtgaagca atcatcgctg tggcatttgg aatatatcca gacaaaatcc cattccaaaa
     7681 ccgcatgatc aaaagccaat acgcgtttgg tacaaaagcc aaagatccca ttgttaatgt
     7741 catccctgct gctgcacgga tcatcatgag ttttcggcct tttcgatcag ctagatttcc
     7801 ccagattggt gcaaccacgg cagatgcaaa agcagtcaca gagattgcta gtcccgaaaa
     7861 caattcgatt tgatcttttg gtgttcctag ttgctcaaca taaactggta tgaatggcat
     7921 aactaaacta atacttgcac cagtgaaaaa acagccaatc cacgcaatca ctaaattttt
     7981 tctccaatta atctccatct atcatccctc tttcgtctct tttatcatac ctattttcga
     8041 ataaacaagc aacgatctcg ccagtaaaaa gagaaaaatc ccgcaaaggt cattttatat
     8101 gtaacgttct ttttatgtaa aacaaaaaga gatcagaaca aatactgttc caatcccttc
     8161 tctttagtta aattgttgct agaatgatga agttgataat gaataaaata tcaacgatcc
     8221 acaaagcaac agaaacttga ttgtattttc ctttgattac tttcacaatt gtatagaaaa
     8281 taaatcctgc tgcaatccca taggaaatgc tatagcacaa tcccataaag atcgatgcaa
     8341 agaaggccgg taaagcttct tccatatcta gccatttgat gtctgcaaaa gaagccatca
     8401 tcattactcc aactaaaatc aatgctggcg cagttgcttg atttggtaca atggcgatca
     8461 gtggagagaa taaactactt aatgcgaata gcactgccac tactactgaa gtcaaaccag
     8521 tacgtccacc ggccccgatc ccagctgcag attcgacata agttgttgta ttcgatgtac
     8581 caaagatagc ccccacagaa gtagcgatcg catcggcaaa tagtgcttta tccatttttg
     8641 ttttgaaccc acgaccatct tccaacgcga tctcgtcttc ttttgagaaa atacctgtgc
     8701 ggcgtcctgt accgataaat gttccgatcg tatcgaaagt atcagataaa ctgaatgcga
     8761 tgatcgtcat caatacttga ggaatcttgg aagaatcact gaataaagat tgcatgcctt
     8821 cagaaccaaa tgctgcaccg aaagttgttc ctaattcttt aaatgaatta cctaacgaat
     8881 tcgtttgcca atcaatggaa cttaaatcaa caacacccat aaaaatacca aggactgtgg
     8941 tcgctacgat accgataaga atagccccac ggacattttt tacaactaaa atggctgtca
     9001 gtaccaaacc aatcacagcc aataagatag gagcgttgtc aaagttggct aacgcaggga
     9061 cgattccacc atttatcgta acatttgtcg cttttccgcc ttcaacgaca gagctcgaaa
     9121 tagctgactg gtctgctgaa aaagataaga atcctgcatt tttgattccg acatacgcaa
     9181 cgaaaatacc gatcccgcca ccaatcgcat gttgaagact ttcgggaatc gcccgaataa
     9241 tcatttttcg aatatttgtg acagtaataa aaatattgat taatccgcaa ataaacacca
     9301 ttgccaaggc ttgttgccaa gaataaccaa gcccaaaaac gaccgtaaaa gtgaaaaatg
     9361 cgttcaatcc cataccaggt gcctgagcat atggtacatt tgcgaacaat cccattacca
     9421 gcgtaccaat aatcgatgca atgattgtcg ctagaaatac tgcttggaag ggcatacctg
     9481 atgctgacaa aatcgtcgga tttacgaata agatataact catggcaaag aatgtcgtta
     9541 ctcccgccat tatttcagtt gatacatttg tattgttttt ctttaaaccg aaaaactttt
     9601 ccataaaaag ttccactccc tgaatttttt tctgatggaa aactataata ataaaaaata
     9661 agtaaaatag ggcaaatcca tcgaacaatt tcaaattata tagacgtttc attcgctttt
     9721 caacgttttt ttaacaaaaa aagcgaaaat aatacttaac gttcgtgttt agcttatgtt
     9781 tagctattta tatagccata actgtagttt ttgttaggaa tagctgattg ctcttgttaa
     9841 ttacctaaaa tatgctagac taaaacaaat gctagttgag gaaggaagga cccttattga
     9901 ataaaaaatt aattgccatc gatctggacg gaactaccct caatcaagat tcgatgatta
     9961 catctaaaac aaaagagact ctaaaaaaag caatcaatgc cggtcatcat gtcagtattg
    10021 ctacaggacg cccattccga atgagccatc agttttacca acaattagaa ttaacaacac
    10081 cgatggttaa ttttaacggc gcattagtac acatccctaa tcagcactgg gatggtgaaa
    10141 gagaaacgtt gatcaatcgt gaaatcgctt ttgaaatctt atcgcaaaaa aagcagctaa
    10201 atttagattt tatcgctgct gaaaatcgtg atactttttt tattgacagt tttgatttct
    10261 ttgatgaaaa aatttttgct tcttctcgtc caggagagaa gaatttgctt tctcctaaaa
    10321 atttgacgac aaatcctaca tctttgcttg tccgaacaga caaacgtttt gcaggagctg
    10381 tatctgctga attaacacgt caatttggca gttatgtcga tgttcggaca tggggtggac
    10441 ctactgccat cttagagatt gtttcaaaag gaatccaaaa agcaaaaggc gtgcaagaga
    10501 ttgctaacta cctgtctatc gatcaaaaag acgtcattgc ctttggtgac gagcataatg
    10561 atttggaatt gttggattat gcaggttggg gagtagctat ggctaacggt actgaccaac
    10621 tcaaaggcat tgcaaatgat gtcactcctt tgagcaatca ggaagacggt ttagctgttt
    10681 atctggaaaa actacttaaa ctataataga gaattagatt ccacaaaaaa gaggttgtga
    10741 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
    10801 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
    10861 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
    10921 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
    10981 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
    11041 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
    11101 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
    11161 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
    11221 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
    11281 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
    11341 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
    11401 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
    11461 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
    11521 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
    11581 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
    11641 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
    11701 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
    11761 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
    11821 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
    11881 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
    11941 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
    12001 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
    12061 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
    12121 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
    12181 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
    12241 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
    12301 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
    12361 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
    12421 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
    12481 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
    12541 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
    12601 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
    12661 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
    12721 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
    12781 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
    12841 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
    12901 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
    12961 ttttgttctt aggttcttgt gcaccttttc ttattttttc taa
//
LOCUS       DACBZN010000080        12989 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.080, whole genome shotgun sequence.
ACCESSION   DACBZN010000080 DACBZN010000000
VERSION     DACBZN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.080"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(88..1668)
                     /locus_tag="IVD64_002064"
     CDS             complement(88..1668)
                     /locus_tag="IVD64_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP6149304.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLYQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2069..3445)
                     /locus_tag="IVD64_002065"
     CDS             complement(2069..3445)
                     /locus_tag="IVD64_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6149305.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3573..4691)
                     /locus_tag="IVD64_002066"
     CDS             complement(3573..4691)
                     /locus_tag="IVD64_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6149306.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4833..5267
                     /locus_tag="IVD64_002067"
     CDS             4833..5267
                     /locus_tag="IVD64_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6149307.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5345..5878)
                     /locus_tag="IVD64_002068"
     CDS             complement(5345..5878)
                     /locus_tag="IVD64_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP6149308.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5972..7150)
                     /gene="mutY"
                     /locus_tag="IVD64_002069"
     CDS             complement(5972..7150)
                     /gene="mutY"
                     /locus_tag="IVD64_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP6149309.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7143..7940)
                     /gene="recX"
                     /locus_tag="IVD64_002070"
     CDS             complement(7143..7940)
                     /gene="recX"
                     /locus_tag="IVD64_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP6149310.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8047..9429
                     /gene="rlmD"
                     /locus_tag="IVD64_002071"
     CDS             8047..9429
                     /gene="rlmD"
                     /locus_tag="IVD64_002071"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6149311.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9603..10181)
                     /locus_tag="IVD64_002072"
     CDS             complement(9603..10181)
                     /locus_tag="IVD64_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP6149312.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10503..10973
                     /locus_tag="IVD64_002073"
     CDS             10503..10973
                     /locus_tag="IVD64_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP6149313.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11081..12307)
                     /locus_tag="IVD64_002074"
     CDS             complement(11081..12307)
                     /locus_tag="IVD64_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP6149314.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3840 a         2621 c         2186 g         4342 t
ORIGIN      
        1 cgtcacggct ctaaacaaaa tatgagaggc tgtttttgtt tggctattct tattacttga
       61 tgcagggcag cttgtgtcaa gacttcttta catcaaactt tttaatttta cattgggata
      121 tttatccgca aaccagcgtt ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa
      181 acggtcacga gccaagatat tgcgacttga actcatcttc tcatccaaat cttctggatc
      241 gatccagcga gcgattttgt gtcccattgg agacatgatt acttcggcgt tatactcatt
      301 taacatacga tattggaata cttcgaattg tagctgacca acagctccaa tgatatattc
      361 ttccgttaga tatgttttat aaagctgaat cgctccttct tgaactaatt gatagattcc
      421 tttgtggaag gatttttgtt tcatgacatt ttttgcagtc actttcataa acagctctgg
      481 agtaaatgaa gggagttctt cataagcaac ttttagtttt ccttcgaaca atgtatcccc
      541 aatctgataa ttgcctgtat catatacacc gatgatatcg cctgctactg ctttttcgac
      601 gttttctctc gaatctgcca taaattgtgt gacattgctt aatttcagtt ttttatttgt
      661 acgttccaac cagacatcca ttcctcgttc gaatgttcca gaacagattc ggacaaaagc
      721 gattcggtca cgatgggctg gattcatatt tgcttggatc ttgaatacga aacctgaaaa
      781 ttcttcctca taaggactta ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg
      841 tgccaattca acgaaagtct ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc
      901 aaaaaatact ggtgtctgat cgccacgagc aattttttct tgattaaatg catctccagc
      961 ctctttgatt agttctactt cttctaagac ttgttcatat aaggaatttt cagttagagg
     1021 atgattttcg ggaagcagtc catcttcatc caattctact aatcgttcat cctgataatt
     1081 ttccggacga taaaactcta cacgattatg gtagatatca taaagacctt ctagcccttt
     1141 acccataccg attggccaat tcatcggata agattcgata tctaatagct cttctaattc
     1201 ttctaacaat tccaatggtt cacgaccatc acgatctagt ttgttgataa aagtaaatat
     1261 cgggatgcca cgttttttta caacttggta taatttcttt gtctgtgctt cgatcccttt
     1321 tgcactatcg attaccatga ccgcactatc cacggccatc aaagtacgat aagtatcttc
     1381 agaaaaatct tcatgtcctg gtgtatccaa gatatttact cgtttccctt gataatcaaa
     1441 ctgcataaca gaactagtaa cagaaatccc acgttgtttc tcgatttcca tccagtctga
     1501 tttggcaaaa ttacctgttt ttttgccttt tacggtcccg gcttgacgga tagccccacc
     1561 aaacaatagt aattgttctg taatcgttgt tttcccagca tccggatggg aaatgatcgc
     1621 aaaagttctg cgattgtcca cttgttgctt taattttgga ttattcataa cctaactcca
     1681 tttcttccat tcaattattt atattttctc taggcacaga ttgtccgatc tcttaaagaa
     1741 agacaatttc atatagggct gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac
     1801 ggcgttctag agacagccta tcagcaataa gcccacccaa gccaaaaata cgaaaagctg
     1861 tttttgcttg gctgctctta ttacttaatg caggatagct ttttcacaac ctttgtcaac
     1921 tactacaact gaatcagtat agcatggatt cagcgaatga aaaagccacc aaaccataag
     1981 agtcaaatat ttttttgtca gctgactaaa agaaaagacg tgttgcttgt ttattcaagc
     2041 aacacgtctt tcataaccaa gtttccaatt atcttcttgg ctcgtcatct tcgaattctt
     2101 ttctgaagta tcttcgttct tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa
     2161 tgacacggat tttaaggatt cgagaacctt ccatttcttc agaaaccagc gtaatattat
     2221 cgacatcaaa cgataacttt tcgccttcat ccggaatcat gcctaatgct gtaatcaaat
     2281 aaccagccat cgtatctaca tcactcatat gcagatcact gtcaaaagct tcattgaact
     2341 catcaatcag catacgtcct tggatgatat attcgtgttc atcgattttt tcataaagat
     2401 tctcaacttc atctgattca tcatcgattt ctccgacgat ttcttctaaa aggtcttcta
     2461 gtgttgctaa cccaacgacg ccgccatatt catctaataa aatagccatc tgattctgcg
     2521 tctttttcat ttcatataac agatcatcaa taaagaccgt ttcaggaacg aataaaggct
     2581 cctgcatgat tttcttgata tctaaattat caaagccgaa tttatgggca gcttttagta
     2641 aattctttgt atgaaggatt ccgactactt tatctttgtc ttcgttataa acagggattc
     2701 ttgaatagtt ttcggataaa acttcattca cattttcttc cacagtgtcg ttgatatcaa
     2761 tcataaatgc atccgttcgt gggaccatga cttctcgggc aactttcgta tcaagagaaa
     2821 aaaccccttg gagcatttct aattcttcat tattcaatac gccttcgttt tcaagcatat
     2881 aacgcatttc atctctggtc atttttgaat cttcatcatc aaaagtcatc ggcgtgatac
     2941 gcgccaataa acttgtggaa gcagaaagca gccagacgaa gggcttagct actacaccaa
     3001 tcaagcgtat cagccctgaa gtgaactttg cgacttcttc tgatttattt aaagcaatac
     3061 gtttagggta caattctcca aaaacaatcg atacataggt caaaatagcc agcacaatga
     3121 tatttgcaat attttttgcc gctgtgccgc cacctaatac cggagctaac cgttccgata
     3181 aagtattagc tagtgaagca ccagacaaaa tgttgaccaa agtgattcct acttggattg
     3241 tcgataggaa attatttgga ttctgcaata tttttaaaag cttttgagat ttcacatctc
     3301 cttcatcagc tttttgctcg attcgattct tattgatcga aacaacagca atttcggaag
     3361 ctgctaaaaa agcattgatc aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg
     3421 actgactctc agggtcagca ttcataatcg cattctctcc ttataaaatt aatttttagt
     3481 ttacgataga aaaaaacgtg gcactatagc cacgttaaaa tggtatcaca aagcaatttt
     3541 ctttgcaata aaaacaagtg tactaaaact acctacgagg tcgtagcaat tttcttcttg
     3601 ttttcatttt gcttgtcttc tttgacgatt ttgattatat aggaaatgat cgtcttgcag
     3661 attgcgtaaa aaggaacacc taagaagata ccaagcaatc cggcaatatt tccagctact
     3721 agaagaacga taatgatcgt taatggatgg attttcagtg atttcccaat tacattagga
     3781 taaatgatat tcccatccaa ctgttggacg attagcacaa ccacacaaca aagtaatgct
     3841 tttaccggat cattgaagac tgttactaga aatgcaggtg ctagtcctag ataaggtcct
     3901 aaatatggaa ttaggtttgt caatcccgca atgaccccaa ataagaaagc ataatccaca
     3961 ccaattacca tatatcctaa aaatgtgaag gtaccaacga ataaacattc gattgcttgt
     4021 ccgctaatgt aattagccaa tgttttattc agttgcccca ataactccac aatttggttt
     4081 cttctttttt ctgggaaaaa ctgttggatg tttggcacta gtttttctcc gtctttcaac
     4141 atatagaaca agatgaatgg tgctgtaatg attactacgg ttgccgaagc gatcgttcca
     4201 acgatcgaac ctaaactatt ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa
     4261 gaaatgtcta attgctcaaa atatttattg atatctactt ccttgaacaa aggcaattcg
     4321 gctacctctt ttaaccagct ttctacctgt ttgatgaaat caggcatatt agatgccaat
     4381 gaggaaattt gctgtactag acttgggatg acactaagta aaatccaaat aattgctgca
     4441 actaaaagga gcagcacaat tgcgacagcc catattcttt tgatttttgc tttcatcaac
     4501 agcttgacaa taggattcaa taaatagtaa agaaagcctg caatcagtac aggtgcaaat
     4561 aacgtggaaa aaaatgtccc gatcggttcg aaaataaagt ttatttctga tgaaatcatg
     4621 atcaatgcag ctatgatcaa taattcaagc gaccaaaaca ttagtttaga ctgtctaagt
     4681 ttttcaaaca atagatttcc tcctttcttt cttactagta tatattatat tcgttaaaaa
     4741 ttcttttatt ctttttttat ttttgaaaac ttgccatact ttttatttgt tttattttcc
     4801 agtatactat acgaaaggaa ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa
     4861 tgagcaatat ttacttagat gctgtacgga tcaggcatca agtttttgta gtcgaacagg
     4921 gtgtgccttt gtctcgagaa atagacaaag atgaagccca ttgtatccac ttcgtcctgt
     4981 actcagataa aaaagaaccg caaggaactg tacgtctttt gcctttagaa aacggaaaaa
     5041 tgaaattaca gcggatggcg attctttctg aatacagaca ccaagggtta ggaaaaatat
     5101 tgattgaaga agcagaaaac tttgctaaga atcaaggcta taatacgatt ttattagggg
     5161 cacagtccac agctgaaact ttttacgaaa aactaggata tacagcttac ggcgatcctt
     5221 ttgaagatgc tggcatgcct catatttata tgaaaaagac tttataaaaa tttatttcga
     5281 aaaaagcaaa aaacagcaag cttttgagaa aaatctctca ctggcttact gttctttttt
     5341 gtgtttactt acgcgacaat tgcttgtaac ggttatacca aatatcaata tagccttccg
     5401 agaacgggcc atctccatta ttgatccagt caaccagaat cttcacattt tcttttagga
     5461 tgaagtcaat atcgttcggg taattcatca ttttgctgtg catctcatat tcatcgacat
     5521 ccaatagtcg tttctcgccg tcaggaaata ccttgatatc caagtcatag tcgatatatt
     5581 tcaaggcctc atcgtccagc aaaaaaggtg aagctagatt acaataatag gaaaccccct
     5641 tttctctgat cattgctatt acattgaacc agtatttttt atgaaaataa acaatcgctg
     5701 gttcgcgcgt tacccatcgg cgtccatcag attcagtgac taatgtgtga tcattcacgc
     5761 cgatcataga gtgttcgctt gtttttaata ccatggtatc tcgccacgtt cgatgcaatt
     5821 tgccgtcgtg cttgtaactt tggatcgtca caaactctcc ttcttttgga actcccatag
     5881 caacccttcc ttaagcagca ttttgtgctt tgtgtttggg tgttcattac accgacatca
     5941 taattattat atcaataaaa atgagctttg cctagaaagg attcctattg tttcgatttt
     6001 tctctaactg atctaccagt ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat
     6061 caggcttcaa ccacttcgaa gttttgttct cggtaaattc ttgttctgct ccctcggttg
     6121 cccgtccgta aaaaaggaga acatgccatt tcagatgact aaaaatatga gtaacttctc
     6181 ctaaatgtcg tgtctgccaa acaaagagtt cctgcttttc aaatgggaga ttttgctctt
     6241 cttctgctac taggtcatca aacagatcga tttctggctt tgtttcccat tcttttttca
     6301 atcgctcata ctcctcttgt gtcacttcca tcattggaaa tgtccacata ttcgccagca
     6361 atttttggct atctctttct tcaaaataat acgctccaga atgattttga agagctgcac
     6421 tgatataata aacgtctttt ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga
     6481 ttcctcgttt gttagctaaa caaaaggctt ggatcggaca agtctcacac tttggagaag
     6541 ttggtgtaca gatagctgaa cctagatcca tcattgcttg gttgaattca cctggatgct
     6601 tttcatcaat gattttccgc atcgcttcat caaagatttt cctgctagaa gcttttgcga
     6661 tgtctgcttc aatgcaaaac aatctgctga ctactcgcat cacgttgccg tcaactgcag
     6721 gttctggaag tccaaatgcg atacttgcaa tcgcgcctgt cgtatatggt cctattccct
     6781 tcaatgagct aatttcttca ggcgtttgcg gcatttcccc atcaaattct gacatgatct
     6841 gtttagcagc agcttggatg ttccgggctc ttgagtaata accaaggccc tcccatgctt
     6901 ttaaaagctt ttcttctgga gcatttgcta gctcttcaat tgtaggaaac cattccatga
     6961 aacgataaaa ataatcgatc actgtgtcta ctctggtttg ctgaagcatg atctcagaga
     7021 tccagatacg ataaggatca cgattatagc gccaaggcag gtttcttttt tcttgttcat
     7081 accattgaat gaactgatct tgaaattctt ttgtttcttt atctgtccat ttttcccatt
     7141 ctttattcac tttcctcttc cttttccgta atgaatcgct ggatcatatc caaatcaaat
     7201 cctttttgat ataagctttg ctttactttt agatttcttt ttttaggttc aaaccttaaa
     7261 tttcttcgcc atagtttatc tccttgtttg actaaataat catattctat ttcttgatct
     7321 gcttcttgcg gaagatcagc tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc
     7381 atcaatgttt gttggatttt tctcaataat tctttttggc tttttccgtg attttttcca
     7441 aacgcttttt cggctgtctg atgtgcattt tgaacttggt cttcaaatgt atattggctg
     7501 agcgcttgtt caatgatttc aggtttcaaa cctttttgct gcatctgctg cgctaatttc
     7561 tttggtcctt tgtcacttaa gcgcatattc gtgcgcacat aactttcccc atagaccaaa
     7621 tcatcgacaa ggtccaattc ttttaagcgg gctacgattt tatttcgatc ttcttgaagg
     7681 atttcctttt cttttaagta tgttcggatt tctttttccg atcgcaactg ataacttaag
     7741 taattcattg ctaggtgaaa accgaaatca gctccgctac tcttcttgat ctcttggata
     7801 gtttcttcgt ctaattcccg tccttttaat aatcgatagc gtactaatac atcttcagat
     7861 acttgtaaag cttctccttc agataaatct acgcgataga aagggccttt gcctttactg
     7921 attctcgtaa cggttaacat aaacatcctc ctcgaacata tattctctat cataccacag
     7981 aaaatccccg taatcaggta ttatatgtta gaataaacca cagaaaaaag aaaaagggag
     8041 atacttatgg cagaaacaat tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt
     8101 ttaggcataa atggtgaagg aatcggttat ttcaaacggt tgatcatatt tgttccatat
     8161 gcactaccaa aagaagaagt actagtgaaa atcacgaaag caactcctcg ctatgcagaa
     8221 ggagaattaa taaaagtcaa aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt
     8281 tactatgaat gcggtggctg tcaattacaa cacttagctt atcaagctca attggatttt
     8341 aaaaaggatc tgcttttgca agcattggaa aaattcaaac cagcaggctt tcgatcttat
     8401 caacttttgc cgacgatcgg gatggatgag ccttggcatt atagaaacaa agcacaattt
     8461 cagttacgaa aaaataagca gactcaaaaa attgaagctg gtttatacga agcaaactct
     8521 catcagttag tccctataac agactgcttg gttcaagaac ctcgaacaca aaaagtaatg
     8581 aatactgttg ttcaactatt gaataaattt gatgcaccca tctatgatga acggacaaac
     8641 agtggaatct tccgcacaat catggttaga atcggtgtta aaactgggga gttacaagtt
     8701 gtttttatta ctcgcagtaa aaaattcccg caaaaaaacg caatgatccg tgagatcaat
     8761 gaacgactgc ccgaagttgt ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc
     8821 gtgatggggg atgaaacgat ccacttatgg ggaaaagaag ccattgaaga acaaatcaat
     8881 gaagtgactt tcgatctttc tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa
     8941 ttgctttatg gcgaaggaat caaagcatta gatgtccagc ctaatgaaac gatcgtggat
     9001 gcttattgcg gagttgggac gatcggctta agtgtagcaa aacaagccaa agaagtacgg
     9061 ggaatggata ccgttcctca agcaatcgct gatgcaaaaa aaaatgcgga aagattagga
     9121 tatcacaaca ctcgatatga agtcgggaca gcagagcaag ttctgcctaa atggttgaat
     9181 gaaggttttc agcctgatgg tatcatcgta gatccaccac gtacgggttt ggataacgca
     9241 ttgatccaat cattattgaa ataccctcca aaaaaacttg tttatatctc ctgtaatgtt
     9301 tctacacttg ccagagatct ggttaattta agtaaaaagt tcaagattga atatttacag
     9361 tccgtcgaca tgtttcctca gactgcacgt tgtgaagttg tcgtgaagat gacgagactc
     9421 gctgactaaa tgaaagaggt tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc
     9481 aaacaaaaat agctcttcat gttttatgaa tactgtactt gtgaccgaag agttaacagg
     9541 aggacactat ttatttaaaa aaagggttgt gacatagttt ttgtcacagc cctttttgat
     9601 tattattgga tattcacgtc tatcacttga taaaatgcat ttgtcgtatc gctgatattc
     9661 catactgcta aaatcacatt atatcctttt cgatcctgcg gaagtgtgat cgtttggttg
     9721 actgtttttg gtggaacagt tgctttgtcg tctatttttg tgatcaattc aaaatcacta
     9781 aatttcaaag gttgattagg attccaagtt ggtttggtaa tgtagtaatc ccaagtactt
     9841 gtccgatgtt gtgctgttaa attccaagta atatttaatg ttcctggatt aaccatgttt
     9901 ttatgccatc tgttagatgt ctgttcatct aaaggctcaa agccgctgac tcctgcacta
     9961 gcaatttttc catcaatgaa tgtatttttg gtagcttcga tactttgcgg ttcccattgt
    10021 gctcttccga cattttgatt taagtttcct gctgctgatg ttcctaggta tgcacggctt
    10081 cctggagaag taatataacc atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa
    10141 ctaactaacc caataaacaa taacgtacta atctttttca tgtagaaacc tccttcattt
    10201 gataaccaca ttgtaaacgc tttattgtat taacacaaac attatgcttt tataatttta
    10261 tgttgaagta aaaaaggtga aaaaagtaag atgcagatta taacagaagt agacgagaaa
    10321 agtacaaata gaaaacatga gaatcaaatc atatgatctt tttcaaaaac atcattcata
    10381 taaacaaatg ttcaactttt taataacaac aattatcaat ttttttctta cttcacaaac
    10441 taaatacagg tcttgctgtg agccttcatg gtaaactaat attatagaaa ggagttagta
    10501 aaatgaagat gataaaagat attcgttttt gggtttgtgt tataggaatt gtcatattag
    10561 gctttttatc agggttgctt agcggaaatc ccggaaaata ttactattct ttgcaacttc
    10621 caccatttgc accacctagc tggatatttg ggccgatgtg gaccttactg tatatattga
    10681 tgggtatttc tctttatttg ttacttaatc acaacaacaa aaaacagcga aacaatcttg
    10741 tcggtctttt tgtgattcag ttcattttta attttatatg gtctgctcta ttttttaatt
    10801 taagaaatat tttcattgca gcaatcgata ttacactttt agttattttc ttgtcagtgc
    10861 ttatgtatca gttgtggctg catcatcgcc tggcaatgtg gctcatgatt ccttattatt
    10921 tatgggtact attcgcaact ctattgaatt attcaattta ttttttaaac taagcgctaa
    10981 aaagagacgt cctgtaattt aacacataga aaaagtgcca cctaagttag attttttgtc
    11041 taatttaggt ggcactcttt attaaccaaa ctattttttt ctagtaccca cttgttgttc
    11101 catactcatt attctggtca taaccataac cattttgttc ctgtccagcg taaggatcat
    11161 aactttgagt atcttgactt ggtgtatagt actgacttgt atcttctgtt tgctgataat
    11221 aggtattcgg atcttggtac tgatcatatg tgttttctga ataggaagaa taatcggtgt
    11281 acccttcact attcgatcca tcaccgtttg tcgaatcgcc atcttccgaa tcatcaaaga
    11341 acatcattga agcattctca tttttcaaat taggtaatgt atcgcttgtt tttatattta
    11401 attgtttctt cagttcattt tggatcgaaa gaagatcatt ttttcctaaa atttgatagt
    11461 agatatcatt gatcatttgt ccttcgcctt gcaactggtc ttgtttgatt gtttcaaacg
    11521 ccggggtgta gttcgtcgct acatcaagca tgtcatccca atctaagtcc gtcgtcacat
    11581 tgttctcaac tgcttttaaa atttttctat aattgcttac gccatctaaa cttaaaactt
    11641 tattgacgat tttcgtcact acttcccgct gacgacgttg tcgaccgata tcgccttctg
    11701 gatcttcgtg tcgcattcga gaataggcta aacctttttc tccattcaat gtttgttcac
    11761 ctactggtac atggacacca tccaaagtaa agtcaatttt attattcact ttgatgccac
    11821 cgaccgcatt gatcaaatct tccatgccat ccaaattgat cgttacataa tgatcaatag
    11881 gaatatcgag cagttgctca attgaatcca tggccatttc gacaccacca aatgcgtagg
    11941 catgatttaa tttatctacc gttccataac ctacgatatt cgtataaata tctcggtcca
    12001 aactgatgat cgttgatttt ttttgtttcg gattaactgt tactaccatc atcgtatcag
    12061 aacgtccttg ttccgttctt cctaatccac cggtatctaa acctaatagc aaaaccgaaa
    12121 aaggttcttt gtcatcaatg cttactctct cagaacgttt ggaggatatt cgattagagt
    12181 ttttagaaat tttgtttact gtttggctcg cttctcccat aaatctgatt ccgtatgctg
    12241 ataatccggc aatcagcaaa acgaataccc ctaacaaggt taatagaact ttttgccatc
    12301 gcttcataat acacctctgt ttcacatttt ctaaaatatt gtctcatata actgttcaat
    12361 cagcaaattc actttttaat cataagaaaa ctttaaaaaa catttttgtg taacgattat
    12421 tattttttaa aatttctcaa aattcttaac tatcatacca taccgtgaaa aagatcgaaa
    12481 gaccgaatac acgcagttcg caatcgtttt atcttttttt atgtattttt tccagaaaaa
    12541 gcgaacaaca aggtattttc caaataatcg tgcagtaaaa tgatcgcaca tacctttccc
    12601 atctttttaa cagtctttgc ctaccgttca gattatcatt cgatttcacg acaatttctt
    12661 cctatgactg gtccttttgt taaacccatc ttctattcgt tttatttcca gctcactgca
    12721 tggctcttaa aatgactcga actagtgatt gcattaccat catcttttaa tggatagata
    12781 tgctcctctt caaagtaaaa cgattttctt ttaaagtaat agaattgaca tggtaatgag
    12841 tatgtatact agtggtttca caaatggtct tttattcaac atccaaaaac tgaaatttct
    12901 ttaaaatcca ttactaacga agaaaatgtt ccttgcttga taattagtta ggacatgtca
    12961 agtgaacaaa tgcatttact tagccaaaa
//
LOCUS       DACBZN010000081        12670 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.081, whole genome shotgun sequence.
ACCESSION   DACBZN010000081 DACBZN010000000
VERSION     DACBZN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.081"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            6..305
                     /locus_tag="IVD64_002075"
     CDS             6..305
                     /locus_tag="IVD64_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6149315.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVD64_002076"
     CDS             378..1739
                     /locus_tag="IVD64_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6149316.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVD64_002077"
     CDS             1761..2021
                     /locus_tag="IVD64_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149317.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVD64_002078"
     CDS             2035..2787
                     /locus_tag="IVD64_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP6149318.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVD64_002079"
     CDS             complement(2881..3486)
                     /locus_tag="IVD64_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6149319.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVD64_002080"
     CDS             complement(3539..4513)
                     /locus_tag="IVD64_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP6149320.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVD64_002081"
     CDS             complement(4891..5670)
                     /locus_tag="IVD64_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6149321.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVD64_002082"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVD64_002082"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6149322.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVD64_002083"
     CDS             7161..8558
                     /locus_tag="IVD64_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6149323.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVD64_002084"
     CDS             8639..9079
                     /locus_tag="IVD64_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6149324.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVD64_002085"
     CDS             9227..9553
                     /locus_tag="IVD64_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6149325.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVD64_002086"
     CDS             9554..10303
                     /locus_tag="IVD64_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149326.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVD64_002087"
     CDS             complement(10383..10838)
                     /locus_tag="IVD64_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6149327.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVD64_002088"
     CDS             complement(11265..11540)
                     /locus_tag="IVD64_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149328.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVD64_002089"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVD64_002089"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP6149329.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACBZN010000082        12581 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.082, whole genome shotgun sequence.
ACCESSION   DACBZN010000082 DACBZN010000000
VERSION     DACBZN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.082"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            46..897
                     /locus_tag="IVD64_002090"
     CDS             46..897
                     /locus_tag="IVD64_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6149330.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            951..2702
                     /locus_tag="IVD64_002091"
     CDS             951..2702
                     /locus_tag="IVD64_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6149331.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            2706..2876
                     /locus_tag="IVD64_002092"
     CDS             2706..2876
                     /locus_tag="IVD64_002092"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149332.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(2916..3818)
                     /locus_tag="IVD64_002093"
     CDS             complement(2916..3818)
                     /locus_tag="IVD64_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6149333.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            4049..5452
                     /locus_tag="IVD64_002094"
     CDS             4049..5452
                     /locus_tag="IVD64_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6149334.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            5452..6885
                     /gene="ascB"
                     /locus_tag="IVD64_002095"
     CDS             5452..6885
                     /gene="ascB"
                     /locus_tag="IVD64_002095"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6149335.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7030..7956
                     /gene="trxB"
                     /locus_tag="IVD64_002096"
     CDS             7030..7956
                     /gene="trxB"
                     /locus_tag="IVD64_002096"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP6149336.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8170..9897
                     /locus_tag="IVD64_002097"
     CDS             8170..9897
                     /locus_tag="IVD64_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6149337.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            9969..10262
                     /locus_tag="IVD64_002098"
     CDS             9969..10262
                     /locus_tag="IVD64_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6149338.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(10354..11214)
                     /locus_tag="IVD64_002099"
     CDS             complement(10354..11214)
                     /locus_tag="IVD64_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP6149339.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            11306..11734
                     /locus_tag="IVD64_002100"
     CDS             11306..11734
                     /locus_tag="IVD64_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6149340.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11896..12072
                     /locus_tag="IVD64_002101"
     CDS             11896..12072
                     /locus_tag="IVD64_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP6149341.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12099..12545
                     /locus_tag="IVD64_002102"
     CDS             12099..12545
                     /locus_tag="IVD64_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP6149342.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4132 a         2147 c         2773 g         3529 t
ORIGIN      
        1 ttttgttttg gatacagccc tatacatcat ggaaaggagc aaaacatgct gattgagaaa
       61 gtactgaata acaacgtcgt aataaccagg gatgaaagaa atcaggaaat gatcgtgatg
      121 ggaaaaggtt tggcattcaa aaagaaagtg ggtgacccga tttttgactc atgcatcgac
      181 aaaaaatttc agttgacaaa tgacgggctt tcacaacagt tccaagaatt ggcacaagcg
      241 attcctcttg agtacttaga aatttcttgt gaaatcattg aatatacaaa agaggctcta
      301 caagtccagt tgaatgattc tgtgtatatt gcattgacgg atcatttgta tacggctatc
      361 gaacgtcaca aaatgggaat atttgtacca aatgtattgc tgtatgatat caaacaacta
      421 tttcctaaag agtatgccat cgggaaaaaa acagtgaaga aaatctcgga taaataccac
      481 atcaagttat cagaagatga agccggattt atcgcattgc atttagtcaa tgcacaaggc
      541 attcaaagcc agcaagacaa tacctacctg tttactataa caatacagga tattctaaat
      601 atcgtccgct attactataa gattacattt gatgaagatt cagtccattt ttttcgattc
      661 gtcacacact tacgtttttt tctgacacgc gtcctagatg gagaaaaatc agaagtggga
      721 gaagtttctg aggaattgtt tcaggttatt caaaaaaaat ataaggatac gtttggttgc
      781 gttgagaaaa tagcagactt tttagccgag acgttcaact accagatgtc cacggacgaa
      841 aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg agaatcgttg taaataaaaa
      901 aaggaggata aattaacaag aacgtatgaa ataagggtag tgacattcag ttggctaaac
      961 cttccactaa taaattggag ggaatagaaa tgggaaaata ccaagaatta gcgagaaaaa
     1021 tcgtagaaaa tgtcggcggg aaggaaaata tcaatggttt gacacattgt atcacaaggc
     1081 ttcgtttcaa attaaaaaac gaagaaaaag caaatgatga gatcttgaaa aatatggacg
     1141 gtattgtaac ggtaatgcga gcgggagggc agtatcaagt tgtcattggg aatcatgtgc
     1201 cagtcgtgtt tgaagaggtt ataaaagcag gaaaccttac ttttgatgag gctgtttcaa
     1261 cagaaaaaat gagaccgttt gatatgctta ttgatggaat atcgggctgt ttccagccat
     1321 tccttgctat cctagctgct ggcgggatga ttcgtggact gacagcattc cttgtcttct
     1381 taggcgcgtt tgaccgcggt tctggcacat ttgttatgtt tgataatatc ggtgattctg
     1441 tattccaatt tatgcctgtt attatcggtt taacagctgc ccgtaaattt aaagtaaatg
     1501 aattcgtagg gatgttgctt ggcgcagcat taatgaatcc tagcttgtcg ctggaagctt
     1561 tgagtggtgc agcagaagca ccactaacga ccatattttc tggcactatt tttgaagcac
     1621 ctatttatca aacagtattc ggcattccct ggattgcgcg taactacgct tcttctgtca
     1681 ttcccattat ctttattgtg ttgttagcta gccaaattca gaaaccaatt aaaaagttag
     1741 tacctgagat gatcgcgaat ttcttcgttc cattcttcac cgttcttatt acaatgcctt
     1801 taggtttttt actcgttgga ccagtattca cattcgcaac tgatatttta atggctggat
     1861 ttgaaacact tttagcactt tcgccagtta tttatggagc aatcgtaggc ttcttctggc
     1921 aaattctggt gatgtttggt ctccattggg caatcgtacc aatgggattg atgcaatttt
     1981 ctgtgaatgg ttggcaaaat attatgacac ctgttgcagt agtgtctttt ggacaaactg
     2041 cagcacttac cgcactctac tttaagctta gaaatccaaa agacaaagca attgcaatac
     2101 cagccattgt ttctggtatt gtggggatta cagagccagc tatttatggg tttacattac
     2161 cacgtaaaaa aatatttatt tttacctgtg tcggtggtgc gttagggggc gcgtatagtg
     2221 ggttaatgaa tttaacttct tggaatcaag gtggactagg catctttaca attcccaact
     2281 atattagacc agatggtgat ttaacagatg tgattaatgt attgattggg atcgccattg
     2341 cgatggtggt atcctttacg ctgacttttt tcttttggaa ggatgaagca ggtgaaacag
     2401 atgatataca gaaaaaatca ggtaaagaaa ttgtaaaaac accgatacaa ggacaaatcg
     2461 ctccactaaa tgcagcaaag gatgcagctt ttgcacaagg aacactaggt cgaggaatct
     2521 tgatctaccc tgaaaaagga gaagtacgtg ccccttttga cggaacagtc atgaccctgt
     2581 tcccgacgaa acaggcaatt ggaatggttt ctgaaacagg gctggaattg ctgattcatg
     2641 tcggattgga tactgtgcag ttggaaggga aatattttga aagcttagtg cagcaaggat
     2701 aaagagtgaa acaaggcgat ctgttgctac gcttcgacaa aaagaaaatc gaaaaggaag
     2761 gatacagttt agaaacacct gtgatcgtca ccaattacac ggattatttg gatattttgg
     2821 aaaaccaagg ggagacggtc gggccagacg atgcacttat caccgcactg acctgaacgg
     2881 ttaggtgaag aatggactag gcttaactat tctttttatt ccacttcttt attttcaaaa
     2941 ttgaaaggct ctttcatgat ggctgagctg atagggcgtt tatcgaattt ttgaccgaca
     3001 gaaattagat ggactaaatt ctgttgataa ttttctgcaa aaacacaact atataacacg
     3061 tctagaagat aacaaattga tgtattgatc gtaaaattac caatttttga atacagtcgc
     3121 tcgcgtgtcg taagtcgcaa agcggctgta ctcattgcag atagcgttga atcaccgata
     3181 ctggtcaaag ctaaaattgg aacatgattt tgtttcaata tttctgcggt acgaaggatc
     3241 acattgtttt cacccgtata agaaatcaga atcccgcaca tatcactcgt gcaattgtac
     3301 gcttcataga atgattcacc aaaagtggtg acaatcgtga catccatttt gatgcgcttc
     3361 attttcagca tgaaatcttg agaaatcaaa gtattcgagt tactggcgaa aatcacgatt
     3421 ttttttgctt gaagcattag ttgtttggct ttttgcagtt ggtcatgatg aatgagagaa
     3481 agtgtatcat gaatcgtcat ctgttctaaa gaagccattt tatttgcgat cgtcataata
     3541 ccgtctgtgg tggaaaaagg aagattcgca tctacgtctt gaaaattccc ttcaagatag
     3601 gctgtttcag ctaaaaaagc attacgaaaa ctgacccagc catcaaaacc catttttttt
     3661 gctacacgga tgagtgtaga ggggtggaca taacatcgct cagcaatcgc ttggatggtc
     3721 atttcgccaa gtgctggaga atcgcttaag agaaactgga tcacttcatt ttctgaagga
     3781 gaaaaggaga tttctttgat tttttcacgt aataacatgt agataccttc ttcttatttt
     3841 tttacgtaaa ttataacttt tttgaaaatg atttgtaaac ggatttatac tatataactt
     3901 aaaatagaca ttttttgaca agatatttac aaaaatccac tagaaaacag taaattttgt
     3961 aaatgttact ggaaagaaac agatttctat tgtttgtttt gtttacaaaa aagataatga
     4021 aagtgtaaaa aagtaaagga gtgagaacat ggcaaaagga atcaaaatcg ctacgattgg
     4081 tggaggcagc agttacaccc ccgagttgat ggaaggattt atcaaaagat atgaggaatt
     4141 accgatacga gaaatctggc tcgtagatgt agaagaagga aaagaaaagc ttgagatcgt
     4201 tagtcagatg tctcagcgta tgtgggatgc ttctccgtat gaagtcaaga tctatgcaac
     4261 tctggatcgg gaagaagcat tgaaagatgc ggattttgtt acgactcagt ttcgtgtcgg
     4321 tcaattagat gcacgaatca aagacgagcg gatccctagt tattatggga tgattgggca
     4381 agaaacaaat ggtgctggcg ggatattcaa agcttttcgt accgttccag tcgttttggc
     4441 aatcgttgag gacatgaagc gtttatgtcc aaatgcttgg ctgatcaatt tcgcgaaccc
     4501 aagtgggatg gtgacagaag cagttatcag atatggcaaa tgggataaag tgattggatt
     4561 atgcaatgtg ccagtgatgg cgatgatgac agaaccagca atgcttggca agaaaaaaga
     4621 agagctgatc tacaagtttg ccggtctaaa tcatttccat tggcacaaag tagcagataa
     4681 taaaggaaat gaccgcacgg cagaattgat tgatcgttta tacgatgaag agaatggatt
     4741 accgaaaaat atctttgata tcccgttttt caaagaacaa ttaaaacaaa tgaacatgat
     4801 cccttgtggc taccaccgct attactatcg tgaagaagaa atgctggaac atgctttaga
     4861 agaatatcaa acgattggta caagagcgca gcaagtgaaa gaaacagaag cagagctatt
     4921 tgaattatac aaagatccag aattagacca taaacctgaa caattgcaaa aacgaggtgg
     4981 cgcttattat tcagacgctg cctgtgaaac gattgcttca atctatggaa ataaagaaac
     5041 acagatcgtg gtatctacaa agaatgatgg agctgttcct gacctgccgg cagattgtgt
     5101 agtagaagtc actgcctatg ttggcgcaca aggagcgaga aatgtagcgt ttggttctct
     5161 tcctgctgct gaaagaggct ggttgcaagt aatgaagaat atggaattgc tgacgatcga
     5221 agcagctgtg actggtgatt atgggacagc tttacaggcg tttacgatca atccacttat
     5281 acgatcagga aagacagcaa aacggatcat ggatgagttg tttattgcac accagcagta
     5341 tttaccgcaa ttcaaggaga caatcagccg attaaaagaa gaaggaatcg aagttcaaga
     5401 tgaactggca agagaattat caggtaatac acatataatg gaggaagtat aatgggattc
     5461 agaaaagatt ttttatgggg cggtgctaca gcagccaatc aatgtgaggg cggttatgat
     5521 gaaggtggac gcggactagc aaacgtagat gttgtgccaa tcggaaaaga ccgttttcct
     5581 gttatcaccg gaaagaaaaa aatgtttgaa tttgatgagg atcattttta tccagcaaaa
     5641 attgcaatcg atatgtatca tcgttataaa gaagatattg ctttgtttgc agaaatgggg
     5701 ttcaagactt atcgaatgtc catcgcctgg agtcgtattt ttccaatggg ggatgagaaa
     5761 gagccgaatg aagctggctt gaaattttat gaggatgcct ttaaagaatg ccaaaagcac
     5821 ggaattgagc cacttgtgac gattacgcat tttgattgtc caatgcattt gatcaaagaa
     5881 tatggtgcgt ggcgttccag agaactcgtt ggtttttatg aaaatctgtg tcgagtcatc
     5941 ttcaatcgct ataaaggact tgtcaaatat tggctgacat tcaatgagat caatatgatc
     6001 cttcatgcac cattcatggg cgcagggctg tattttgaaa aaggagaaaa tgaagaacaa
     6061 atcaaatatc aagcggcaca tcatgagttg gttgcaagtg ccatcgcaac gaagattgct
     6121 catgaagtag atccggagaa caaagtaggc tgtatgctgg cagcaggagc atattatcca
     6181 tacacttgca atcctgaaga tgtttgggct tctagaaaat cagatcgaga aaattatttc
     6241 ttcattgatg tccagtcacg aggagaatat cccgcttatg cattgaaaga aatggagcga
     6301 aatggcattc agatcgaaat ggaaccaggg gatgccgcat tgctgaaaga acatactgtt
     6361 gattttatct ctttttctta ttatgcttcc cgagtttcga ctaccgacaa cgaactcttg
     6421 gaacagactg caggaaatat ctttgcttct gtcaaaaatc catatctgca agcaagtgaa
     6481 tgggggtggc aaattgatcc attaggcttg agaataacga tgaatgatat ttatgatcgt
     6541 tatcaaaaac cattgtttat tgtagaaaac ggcttaggtg cagtcgatac gccagatgaa
     6601 aatggttatg tggaagatga ttaccgaatc gattacctgg cagcgcatat ccaggcgatg
     6661 aaggatgcag tagaactaga cggagtcgat ctattaggct atacgacttg gggatgtatt
     6721 gatttagtat cagctggtac cggtgagatg aagaaaaggt acggattcat ttatgtcgat
     6781 cgtgataacc aaggaaatgg cacattagcc cgttcaaaga aaaaatcatt caactggtat
     6841 aaaaaagtaa ttgattcaaa tggagaaaat ctttcgaacg attgatcaat gatttataaa
     6901 agtctattgt ttttcagcgg atttttcgag tataataaag ctattcgaga aacgcaagca
     6961 gaacaataga ctttttttcg cattggctgt aaagtatgct tacttgatta agtaaaggag
     7021 aatctacata tgtatgatgt aatcgttatt ggcgcaggtc ctgctgggat gactgctgct
     7081 ttatatgctt cacgttcaaa cttatccgta ttgatgatcg aacaaggtgc ccctggcggc
     7141 caaatgaaca atacggcaga aatcgaaaat tatcctggat tcgattcgat catgggcccc
     7201 gatctagctt ataagatgta tgaaagtgtc tcaagatttg ggacagaaaa tgcttatgga
     7261 atcgttcaag atatcaagga tcacggcagc cataaagaag tcatctgtga agataaaagt
     7321 tacgaaggaa aaacagtcat tatcgcaaca ggatgtatcc accgtaaatt aggcgtacct
     7381 ggcgaggaag aatttgctgg acgaggcgtt tcttactgtg cagtctgtga tggtgcgttt
     7441 ttccgtaaca aacgtctagt agtcatcggg ggtggggact cagcagtaga agaagccatc
     7501 tatttgacac aatttgcttc tgaagtagtg atcgtccacc gtagagatga attacgtgca
     7561 caaaagatca tccaagaccg tgcatttgca aatgaaaaaa tttcctttat gtgggataca
     7621 gtcgttgacg aaatcgttgg aaacgaaatg gtagtgacag gtgtgaaagc acgtaatgtc
     7681 aaaaccggca atgaatttga tttagcagca gacggagtat tcatctatgt tggactggat
     7741 ccattaactg caccttttag agaagcaggt ttgacgaacg aagctggctg gattccaaca
     7801 gacaacgaaa tgaaaacgaa tatgccaggc gtttttgcca tcggagatgt tcgtgaaaaa
     7861 gatttgcgac aaatcactac agctgttggt gacggcggta ttgctggaca acaagtgtat
     7921 aaatacttgg agtctttaga agaaacaaca gcataagttt aaaaagagta gaagaacggg
     7981 ttcaattgtt cttttactct tttttttcgc gtgaaattta ctgaatctga tttatgcgaa
     8041 cacgtccaac accttttgat aaaagaatcc tttcttgatt taagcgtttt ttggtcctca
     8101 tactttcata tctaataagt gaatgctata cttattaaga accatatttc agaaagaggt
     8161 gttattttca tgtcttggga acaagtgtat gaacaatggg tacatgaaga aaatttagaa
     8221 gaaaacttaa aaaaacaatt aaatgatctg ggtgaagatc ctgaaaaact tgaagatgct
     8281 ttttatgcac cgttggaatt cggtactgca ggtatgcggg gaatcttagg acctgggatc
     8341 aatcgaatga acatctatac ggtaagacaa gcaacagaag gactagcacg tttcatggat
     8401 acacaaggtg aagaaacaaa acgccgaggt gtcgctatcg cttatgactc acgccatcag
     8461 tcaccagaat ttgcaatgga agcagccaaa acactagcac atcacaatat cccgtcttat
     8521 gtattcgaaa gtctgcgtcc aacaccagag ctttcttttg cagttcgtca tttgaagacg
     8581 tttgcgggaa tcatggtaac cgcatctcac aacccagcgg cttacaatgg atataaagtc
     8641 tacggagaag atggcggaca aatgcctcct gcagacgcag atgcattgac gaaatatgtc
     8701 cgtgaagtca gcaatccgct aaaagtcgaa gtattatctg acgaagaagc taaacatagc
     8761 gggttgatca cgatcatcgg agaagaagtg gacgctgctt atctagaaga aatcaaagca
     8821 gtgacgattg atcgtgaatt ggttgaaaca atggggaaag atttgaaact tgtgtacacg
     8881 ccacttcatg gaacagggaa aatgttgggc gaacgtgcgt tgaaacaagc tggattcgaa
     8941 caattcgtac tagttcccga acaagctgtg gcagatccag atttcactac ggtcaaatcg
     9001 ccaaatccag aagaacattc agcttttgaa tatgctatac gacttggaga aaaagaagga
     9061 gctgacttgc tgattgcaac agatcctgat gcagaccgtt taggcgcagc ggttcgtttg
     9121 ccagatggtt cttatgaagt attgactggg aaccaaatcg gagcattgtt tgctcaatat
     9181 attttggaag cacatcaatc aagaggcact ttaccagaaa acgcagcggt attgaaatca
     9241 atcgtttcca gcgaattgcc tacagcaatt gcggaacact acggtgctac catgttcaat
     9301 gtgttgacag ggtttaaatt cattgctgaa aaaatccaac aatttgaaga agaccattca
     9361 catactttta tgtttggatt cgaagaaagt tatggttatt tagttaaacc ttttgttcgc
     9421 gataaagacg cgatccaagc attgctttta ttagcagaag tagcagctta ctataaaaaa
     9481 caaggcaaaa cgttgtatga cggcttgcaa acgatttttg aaacctatgg ctatttcgca
     9541 gaaaaaacga tttctgtgac aatgagcgga caagaaggtt ctgcgaaaat ctctgctttg
     9601 atggaaaaat tccgtaaaga agcaccgaca gaatttgccg gagcaaaagt tattcaaaca
     9661 gaagacttta aagaattgac gagaacagac cgtgaaggaa acgtagaaaa actaacaaca
     9721 ccgccatccg acgtattgaa gtatacattg gaagaaaatg gctggatcgc tatccgccct
     9781 tcaggaactg agccgaaaat caagttttat attggtgtaa aaggaagctc tacagaagac
     9841 gcagccgaaa aagtagcctc ttatgaagcg attatcaaaa agatgacaga agaataaaaa
     9901 acttgagact gatggaaaaa gtggtaaact ttagaagtca gtcttttttc aagttggagg
     9961 tgaatggatt gccagacacg caaaaatcaa aagtagagaa agtcagtcaa ttattcaaaa
    10021 tgttgagtga tcccactcgt cttaaaattc tgctatatct gaaagacggg gaacaaaatg
    10081 tgacagcaat cacccaagca gtggaaatgg agcagtctgc tgtatcgcat caactacgtt
    10141 tattaagaga aaatcatgtc gtcaagtctc atcgcgaggg aaaagcgatt ttgtatagtc
    10201 ttgatgattc tcatgtactg gatattttga accagacact caaacatgtg gaacatcaat
    10261 aacgataggt gaaaatagat aaacactgga gtgtgacaac gaagaaattt cgttgtcaca
    10321 ctccagtgtt ttttaagaaa aagacaaaaa tgattaatcg cttttttctg ttgtttcagt
    10381 tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagcgaag ccgtttcttc
    10441 aatagataaa gatgccacat tgatcactac acatcctagt ttttcataaa gatcattcgc
    10501 aaaatcgagt tcagctctaa ttttttcgat atcggaataa gcagtatcag ggtttaatcc
    10561 gtaagagcgc atccgttctt tacggatatt atttaaaata tctgggttat tcgttagtcc
    10621 cacgattttt ttaggatcga tttcccacag ctgtttggga atatgtgctt ggggaatcaa
    10681 aggtaaatta gcaactttta aatttttatt tgctaaaaac aagctcaaag gcgttttgga
    10741 agttcgggat acgcctagca aaacgacatc tgcttctaaa aaaccacgag gatctttccc
    10801 atcatcgtat tttacagcaa attccattgc tttgatccgt ttgaaatagt tctcattcaa
    10861 taaatggagg gcaccaggtt gccgaattgg cttgatacca gttaaacgtt caatttctga
    10921 tatcggtggc gtcatcacat cgaaactaaa taattgatgt tcttcacaaa aagaacgtgt
    10981 tatctcgatc agttcttgat tgattaatgt atgcaggaca agtgcttttt cttctaatgc
    11041 atcttgcaat gcctgcctta atgttttttc atctgtcacg aacgttctgc ggaataggtt
    11101 gaactcaacg gtaggatact gagccatagt agcttgcgct agttttgtcg cagtttctcc
    11161 agcagagtcg gagatgacaa aaaaagtaac gatttctgta gaatcggaag acatggataa
    11221 aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgcg tacttcgtaa
    11281 tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgatcgaac aatcattaga
    11341 aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt taacttattt
    11401 agtgaagcgt aaccgctacg tatctgctaa agaagttttt gactatatga atcaggaatt
    11461 caaaggggta agttatgata cgatttaccg gaatctccat gattttgcag agatcgattt
    11521 actggaagaa accgatttaa atggtgaaat gaagtttcgt tttcattgct gtcaggggga
    11581 aatcggccat caccatcatt ttatctgtac agtgtgcgga acaacgaaag aaatccatat
    11641 gtgtccaatg aattatttcg aagaacagct aggtggatgc acgatcgaag ggcatagatt
    11701 cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata acatttgata
    11761 atttaaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat aatatttaca
    11821 ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt ttaagctcgg
    11881 agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact tgacgatgct
    11941 cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc tcgtaaacgt
    12001 gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc tagaaaacgc
    12061 aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta agtagattaa
    12121 acgatgacat gaaagcagcg atgaaagcga aagataagga atctttacaa gtcatccgaa
    12181 tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg acagaagaag
    12241 aagaactaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg catgagtttg
    12301 aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgta atagtcgaaa
    12361 aatatatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa gaagcaatca
    12421 ctcaaactgg tgcttcatca atgaaagaat ttggcaaagt tatgggtgca attatgccta
    12481 aagtcaaagg aaaagcagat ggaaaccaag tgaatgcgat tgtgaaagaa ctattacaat
    12541 cttaatttta tcatttaaac tgcggacagt gattgtccgc a
//
LOCUS       DACBZN010000083        12575 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.083, whole genome shotgun sequence.
ACCESSION   DACBZN010000083 DACBZN010000000
VERSION     DACBZN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.083"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(3..2012)
                     /gene="metG"
                     /locus_tag="IVD64_002103"
     CDS             complement(3..2012)
                     /gene="metG"
                     /locus_tag="IVD64_002103"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP6149343.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2280..2558
                     /locus_tag="IVD64_002104"
     CDS             2280..2558
                     /locus_tag="IVD64_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149344.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2655..3476
                     /locus_tag="IVD64_002105"
     CDS             2655..3476
                     /locus_tag="IVD64_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6149345.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3761..4771)
                     /locus_tag="IVD64_002106"
     CDS             complement(3761..4771)
                     /locus_tag="IVD64_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6149346.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4783..5736)
                     /locus_tag="IVD64_002107"
     CDS             complement(4783..5736)
                     /locus_tag="IVD64_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6149347.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5726..6796)
                     /locus_tag="IVD64_002108"
     CDS             complement(5726..6796)
                     /locus_tag="IVD64_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149348.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6802..7845)
                     /locus_tag="IVD64_002109"
     CDS             complement(6802..7845)
                     /locus_tag="IVD64_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6149349.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRVKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7846..8787)
                     /locus_tag="IVD64_002110"
     CDS             complement(7846..8787)
                     /locus_tag="IVD64_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6149350.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8871..9218
                     /locus_tag="IVD64_002111"
     CDS             8871..9218
                     /locus_tag="IVD64_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP6149351.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9288..10949)
                     /locus_tag="IVD64_002112"
     CDS             complement(9288..10949)
                     /locus_tag="IVD64_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6149352.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(11495..12043)
                     /gene="aac(6')"
                     /locus_tag="IVD64_002113"
     CDS             complement(11495..12043)
                     /gene="aac(6')"
                     /locus_tag="IVD64_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6149353.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3573 a         2555 c         2238 g         4209 t
ORIGIN      
        1 ttctatgcga tttctgagcc atttggcatg gattttggtg cttcgattac ttgtaaagag
       61 ccatctggtg cttctgcaga aaggatcatt ccttgactga tttgtccacg catctttcgt
      121 ggtttcagat ttgctacaat gacgactttt ttcccgatca attcgcttgg atcaggataa
      181 aattcagcga ttcctgacaa gatttgacgg tcttggctgt ctccagcatc tagtcggaat
      241 tgcagcaact tatctgcccc ttctactttt tgacagttga tgacttctgc tacttttagt
      301 tctacttttt caaatacatc aaatttgatt tgtttttctt tcgttgaaac aagttctgtt
      361 tcttctggat cccatttgac tgtatcttcg tttgtttgag taccttcaga cattttttct
      421 tgaataaaag caacttcttc ttccatatct aaacgtggga agattggtgt accttttgca
      481 actacttttg ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt cattgtttca
      541 gagtccaatc ctagttgatt gaagatcttc tctggcgttt ctgtcatgat tggctgaagt
      601 aatatcgctg cgatacgcaa agattcagct aaatggatca tgacactatt caattcagct
      661 ttcttttctt cgtcttttgc taatacccat ggttctgttt catcaatgta tttgtttgca
      721 cgagaaatca gcacccagat ttcagccaat gctgtattga attccatttt ttccattgca
      781 tcatggtaac gtccgacaac gttagcagcc gtcgtagaca gttcactatc aaatggagtg
      841 acaagtgagg catagtcagg aacgagtcca tcacaatatt tgttgatcat agcaatcgtt
      901 cgattcaata agttccctag atcatttgcc agatcataat tgacacggga aacaaaatct
      961 tctggcgtaa agacaccatc tgacccaaac ggaacagcac gaagcaaata ataacggagt
     1021 gcatctaaac cataacgttc cacaagcatt tcaggataaa cgacatttcc ttttgattta
     1081 gacatttttc cgtctttcat caatagccag ccatgcccaa atactttttt aggtagaggc
     1141 aaatcaaggg ccatcagcat gattggccaa tagatcgtat gaaagcgaac gatttcttta
     1201 ccgaccatct gaacatctgc aggccagtat ttttcgaata gactttcatc ttcggagcca
     1261 tatcctagtg cagtaatata attggataat gcatcgatcc atacatacac cacatgtttc
     1321 ggatcactct tgactggtac tccccaagta aaagttgttc gtgaaactgc aagatcctct
     1381 aatcctggtt tgataaagtt gttgatcatt tcgtttttac gagattctgg ctggatgaat
     1441 tcgggatgtt cttcatagta ttccaataga cggtctgcgt atttgctcat gcggaagaag
     1501 tatgattctt cttttaccaa ttctacttcg tgtccacttg gcgctttacc accgatcacg
     1561 ttaccttcgt catctttgta cacttctgca agttgtgttt ctgtaaagaa ttcttcgtca
     1621 gaaacagagt accagccttc atattcacct aagtaaatat ctccctgttc taataaacga
     1681 tcaaatattt tttgaacagc tgctttatgg taatcatctg tcgtgcgaat gaatttatca
     1741 taactgatat caagcgtttt ccataatttc ttgacatcag ccgccatttt atctacatat
     1801 tcttgcggtt ttacacctag ttcttctgct tttgtttcga ttttttgacc atgttcatcc
     1861 actccagtga gataaaacac atcaaagccc atcaaccgtt tataacgagc aattgcgtca
     1921 catgcaatcg tagtatacga gttgccgata tgaagtttgc cgcttggata atagattggc
     1981 gtagtgatat aaaaagtttc cttttctgcc acggggctct tcctccttta tcgcatgatg
     2041 cgaattcgtt tccttgctag tataccataa aaaaagcatg ctgttagctt tttttcaaaa
     2101 tgaaaaagac tggataaacg acttctatct acgttcaatc tatcctttaa aaggatgaat
     2161 aggttgaata aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg gctttttatt
     2221 tctcccctca cacgtggtat aataaagact atctatctta gtaagggact ggaacaatca
     2281 tgcagaagaa acatcttttt tttacactta gtattgcttt tctttcatta gcacatttaa
     2341 tattcagcta tttttatatt cgtatgtatg gttattttaa cctccatggg catttgaaca
     2401 gttttatgac agcagcttgg attttgcgtt ttatcattga cgtatatatc gttatttgtg
     2461 gcttctttgc cattcgtgaa gagcggtata aagtattgcc gttttatctg cttttctttt
     2521 tattcaatct catacttcca tttatttttc atatttaaat gataacctag atttttcaag
     2581 taaatccttg gttttttttc agagtctgct acaataaggt aaaaagtact tttactgtca
     2641 atggaggtgg tcagatgaac cgttttgctt ctaaagaaga agaaaaatta tattatgaac
     2701 ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt ccagaatatg
     2761 acaagccctc tttgactgtc gatatcgtac tgatgtgcta taacaaagaa gaagatcagc
     2821 tgaaagtttt gttgatccaa agaaaagggc atccttatcg aaattcatgg gcattgcctg
     2881 gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa acaaaggaag
     2941 aaactggtgt ggttatttca aaagaaaata ttgagcaact gcatacattc agtacgccta
     3001 atcgagatcc gcgaggctgg gtcgtcacag tcagctactt agcctttatt ggagaagaac
     3061 cgctaatcgc cggagatgat gccaaggaag tacgctggtt cacactagaa cgtcatagta
     3121 acaagatcca tttatctagc ggagaagtgg ctatctcgct tgatttagtc actggagagt
     3181 cttctggcaa agatactttg gcttttgacc atagtcaaat catcttgaaa gcattcaacc
     3241 gtgtagtcaa taaaatggaa cacgagccgc aagttctaca agttttaggt aaagatttca
     3301 cgattacgga agctcgaaaa gtatttgcta aattcttagg gatcgattat aaaacgatcg
     3361 atcattccaa ttttaagaaa gctatgctcc aatatttcga tgagatcggt gaacgtcctg
     3421 taggaatcgg ccgtccttca aaaatctatc agctcaagcc ggggcaccat gaataaggtt
     3481 gtgagcaaaa gattcagacc taatcattca agaaaaaacg caaaaacaac tctttgtgag
     3541 ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa aaaattaaca
     3601 atagcagaaa aagctcgtac gaactttttc tgctattgtt ttattggttt gtcataatta
     3661 gtgagctttt cttcaaaaga aaaattccta gaaacaaaag tcattaagac tttttattaa
     3721 caaggctaac cctaacgctc ctatcagttt cattcatttt ttatcccttg ataaccgaat
     3781 gtctgcccat tttttctact acttggctct ttcttacgga attgctttct tctgctccgt
     3841 cggtattgat caaaagaaat tttttattat accattggga cttataacgc aattttgtta
     3901 ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt ggaaaagatt
     3961 ctacaaataa gtagtcatat tgtttgacca atttcccaga taattgttca agataatctt
     4021 ctcgctggtt cttttgtttc agataaagat ccattacttt tctcttttgt ttctgataat
     4081 tgcttgcctg atctaacctg atctttctat ggtgagcgga gcggactttc acattcaatt
     4141 tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa gttacttcta
     4201 gaggtcgaga tgtttggatc aatgtcttcg gatgataggc gatcccgatc catttagcta
     4261 aagattcttt ttttactggc gaaacatcac aaagtagtgc gacataaaat tcttcattat
     4321 atcgagcaga aatcgtagcg gagcgaatcg atccttcaac tggccgatgc tgatttatct
     4381 tgattagttc tttttgtttt ggtaacttta agtagccatt ttccagatag atcgtacggg
     4441 tttggttgtt ggtcgtataa gattgccaca tacttttttt cgttttaagc tttgggaagc
     4501 tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa attaagctgt gcatttgcta
     4561 aagcaagact gtctacttct tttaagaatg ggtattcttt ttttaatgta gcgggtgtga
     4621 gcgaaaaatt ggctactcct gtttgttgcc aacttttctg ccgctttttc agcaaatggt
     4681 tataggtaaa tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt tcttctgtcg
     4741 gatacatacg gaatttaaaa gcttttagca tctatgtccc tcctagttag caaccagaag
     4801 agattccttt tgtcgtttta gtttttttgc gtaatatggg acttcctgct cagttgcata
     4861 gacaaaatgt tctggtgcga tttcacgaag ttgacgtact tgtccgtcat ctggttcagg
     4921 ttgatatttg attcttctgc gttttctctc atggtctgga tctggtagag gaatagcaga
     4981 taacaaactt tctgtataag gatgaacacc gtgattatag atttcatcac ttgttcccat
     5041 ctctaataac aagccgttat gcattacacc gattcggtca ctaatatgtt taaccatcga
     5101 caaatcatgt gcaataaata gataggtcaa ttgatgttct ttttggagat cttgcaacaa
     5161 attgaccact tgggcttgga tcgacacatc tagtgcagaa attggttcat cacaaatgat
     5221 aaaacgcggt tttactgcca atgctcgtgc aataccaata cgctggcgtt gcccacctga
     5281 aaattcatga ggataacgag taccgtggct tggattcaat ccaacagttc tcaatagatc
     5341 atcgactttt tttgcacgct cttcagatga tttagctagt ccattaacat caattccttc
     5401 ggcaatgata tctctgactt tcatacgagg attcaacgaa gcgtatggat cttgaaagat
     5461 catctgaaca tcatgccgga atttcgtcaa acctttttta tctttgatct tcgttatatc
     5521 ttgaccgtcg aataatatct caccatcagt aggttcattc aagcgaatga tcgtacggcc
     5581 agtagtggat tttccgcttc cagattctcc aactaaaccg aatgtttctc cttcataaat
     5641 gtgaaagctg atgtcattta cagcatgtac ttcattcttt tttcctacat tgaaatactg
     5701 tttcaatcct ttaacttcaa gtagtttatt tggcatcagc aattcctcct tgaccgtaag
     5761 tatgtttttg tttctcttta aagatagccc agcgacgttg gatttcttct ggcggtgtca
     5821 ctttgggtgc ctgcggagca agtagccaag ttgctgcctt gtgtgttttt gatagttcaa
     5881 aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt ggatagaatg
     5941 cgtctccttt tggcgggttc aataaatccg gtggagaacc aggaatagca tacaatcgat
     6001 cgttcgcact atctaatgtc ggcattgaac caagcagtcc ccaagtatag ggatgttgcg
     6061 ggttgtagaa aatttcttcc gatgttccaa cttcaacgat tcttcctcca tacatgacag
     6121 cgacacgatc agcgacattc gctactacac caaggtcatg agtgataaag ataattgatg
     6181 tatcgatttt tgtctgaatt tctttcagca gctccaaaat ctgtgcttga atagtcacat
     6241 caagcgctgt tgtaggttca tcagcaatca ggatttccgg ataacatacc agtgcaatcg
     6301 cgatcacgat ccgttgacgc tgacctcctg agaactgatg cggataactg ttgatacgtt
     6361 tctcagcttc gggtattcca actaatttaa gcaattctaa tgcagctttt tgtccctctt
     6421 tttttgaaac tttattatgc tttctaaggg actcagcgac ttgcttccca atgggcatcg
     6481 tcggatctaa agaggtcatt ggatcttgga aaatcatcgc tatttcttta ccacggattt
     6541 tttgcatctc tctttctgat tttccaacga tatcgttacc tttaaagagg atttgaccag
     6601 catctatatt ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc actgattttc
     6661 cacttccaga ttccccaacg atcgccaatg tttctccttt attcaaatga aaattgacac
     6721 cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga gatttctaag tctttcactt
     6781 ctaaaatttt cgtcatcgtc ttcactcttt cattttagga tcaaaggcat cacgcaatcc
     6841 atcagcaagt aagttaaagc caatcatgat aaccgataaa gttactgccg gaatccataa
     6901 tagataaggc agatacagga atgtcttgta cccttcgctc aacatcgttc ctaatgaagc
     6961 tgacggaggc gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga aaatagcaga
     7021 tggaatacta aacatcattt gaataataat gacactagag atattcggca aaatatgttt
     7081 gaatgcaatt ttcattcttg attcacctaa tgtcaatcca gctaaaacat attcttgatc
     7141 ttttaatttc attgtttgtg cacgaacgat acgtgccatt ggtatccagt ttgtgatggc
     7201 catagcagcc acgattgaaa aaattcctgg ttcaaaaacg accaacatca aaatcatgac
     7261 aacgaggttt ggaattcctg ataagatttc caaaaaacgt tgcatcacgt tatcgacacg
     7321 tccgcctaac aaaccagaaa ccaacccgta agctacacca atcgttacat ctagtaatgc
     7381 agcaataaat gcgatcagca acgagattct cattcccata aataagcggc ttaatacatc
     7441 acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt ttgcttgagc
     7501 atatttatca acaagttcac caccaaccat tgcttttcca ttcaatccat cgatattgat
     7561 tcccggaata cgaggaggca aattgatata agccacgttt tgggcagttg gatcatgagg
     7621 agaaacaaag attgtgatca cagagatcaa aatgatgacc aatagtaaaa ccattgaaat
     7681 aacggcagct ttgtttttct ttacacggcg ccatgaatct tggataaaat tcaaagaagg
     7741 tgcagtaatc tgttcacgtt cttcttcggt atttttttgt aatggaagaa attcatccgc
     7801 agggatatct gcaactgttt cataacgctt tggtatcatt tccatttatc ctcggctccc
     7861 ttctgaaaca cgaattcttg gatcaactag accgtacaat agatccacga tcaaaataac
     7921 aaaaatcagc ataaatgagt aaaggatcgt tacagccatg atcgtaggat aatcatttgt
     7981 tgtgattgac ttaacgaatt gttccccgat accaggaata gcaaagatat tttctaccac
     8041 cagtgatccg gtcatcaatg ctacagctaa cggtcctaat aaagtcatca atggaatcaa
     8101 actatttcga agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt ttgcttttgc
     8161 taattccaca taatcactat gcagtacttc caccatttcc gtacggataa accgagcaga
     8221 gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca caaagccatc
     8281 ccattttgcg attggcaaaa cttgtaattt gattgcaaag atatattgta ataaaacagc
     8341 aaatacaaag tttggaatgg aacgacctaa tatcgctacg agtgtagaag atgtatctgc
     8401 ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc gtccctagga ctgttccaaa
     8461 gataattgcc tgtaacccta actgcaagga tggtccgatt cttccagcta acaaatgtgc
     8521 gactggctgg tttttaaatt ggaaggaaat tccaaaatct ccttgaagca aattagaaag
     8581 ataaatccca tattgtacga taactggttt atccaaaccg acttgtttat ttagcatctc
     8641 gattgtttca ggactcaaac gttcctgatt cgtatatggc gtgcccggca gcaattgcat
     8701 caagaaaaag gtgattgttg caattaacca aagcgtgatg atcatgaaaa agacacgttt
     8761 taatacatat ttgatataac tattcactaa tgtttccccc tagcttttga cttctgacag
     8821 atgtttcgct acattattag ataatgaacc aaaaataaag gagcaatcaa atgaaaaaaa
     8881 aatctatccc ctacgcagta gcttttctac tgattctggt tattttaata aaaaatctca
     8941 tcaaccattc ctttacactc attcaattat ctaatgatct attcttatgg tcattgccgt
     9001 ttctgattat cggcggtttt ttatgggtct tttcttcggg tttcttcgat cattttcagc
     9061 gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa accagaattt tcctcgctct
     9121 cgtctgccag ctacggtatg tacagctttt ggctgatcat tgccggaata ctaattgctc
     9181 tttctgctat tttcatgctt ttttctttgt taggataagt gaatatgaga acgaggttgc
     9241 gacatttgcc gcagcctcgt tttttctcat gctcacggta ttaacgatta ttcttcaacg
     9301 tatgtccatt tgtaatccca tgaagcgcct gccgcatggt gaacgatacc tttgattttt
     9361 tcattcacta ggtgaccttc tgctttttgg tacaccggaa cgacacccat atcatcagag
     9421 atgatcttga ttgctttttg gaaatcatcc caacgggctt gttcatcgcc agcgtctgtt
     9481 gatgaagctg ctttaactgc ttcgtcatat tctgcattgc tccagcgacc acggttgtat
     9541 gaattacctg tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc agcagcccat
     9601 ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac agagaatgga
     9661 acaggtgttg gtttgactgt tacgccatcc aagttttctt ggattgagtt ttgaacatat
     9721 tcgattactt ttttcgatga atcatcatca gaagccatca aatcaaattc taaagaatca
     9781 acgcccaatt cttttttcgc tttttcccaa tattcttttg ctttatcttt gtcataagaa
     9841 actaattttc cagcttcttt tgcaaagtct tcgttcgttg ttggactttt taccatatct
     9901 gatgggatca atcctgtaga gacaactgat ccgtcaccta gtacttgttt tactaaagct
     9961 tcacgatcga tagaataaga aatagctttt cttaggttca cattgttgaa tggtgaattc
    10021 ttttcacgtt gattgaattc gatatagctt gtccgcgctt ctttaacaga agtataagct
    10081 ggatcgtttg cgttttgctg tgctaactca ccagacaaga tcacgtcatc tgcttggcca
    10141 tctttgaata agttcaatgc agtagaagat tcttttacta cactgacttt gatttcagaa
    10201 agttttactg tatctttgtc ccagtaatga tcatttttct tataagtcca ttctgtatct
    10261 gttcctggtc catcaaaatc ttctaatgtg aatggtccat tataaacagc tttatcactt
    10321 gcagatgcat aggcatcccc attttcttcc actacggatt gtttttgtgg gaaaaatgat
    10381 gggaaagcaa gcagatattg gaaatatggt gtttgtttcg tcaatttgat ttctaattca
    10441 tagtcaccaa ctgctttgat ccctagttct gatttatctt tctttccagc tgtaatgtct
    10501 tctgcatttt caactggcgc atacaggtaa gcatattcag aagctgtagc tgggtctact
    10561 gttcgttgcc agccataaac gtaatccgct gctgtaacag gatcaccatt tgaccatttt
    10621 gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt cactgacttc tgccaactcg
    10681 ctagctcctg ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc ttcataaacg
    10741 ttattcaatg ctgtaaaact gattgtatct gttgcaactg ataagtcagc tgtaggcatt
    10801 tcttgaggca cgactaagtt aaatacgcct cctccatctg ctgaaccgct ttttgctgaa
    10861 ctactgcttg tggctgaact gccgccataa catccagcaa gtacaaggcc gcataccgca
    10921 accaaaccaa atgtaaattt ctttttcatt ttacgtaccc ctttattgga attttcagaa
    10981 taattaatat tttctgaagt tgtttaagag tttaatgaat aattattaaa aaatcaacac
    11041 ttttttttgt ctttttcata aaataatgat tttttcgtga ggtttcaagg aatttttcag
    11101 aaaaaatagc tttgtacaag gttttccatg gtcttccaaa agccattaaa taattttcgt
    11161 tatattgtct attattgcca tacaaagata aaaaagaata aacaaaaaga aactgtgaca
    11221 aaagtcttgt cacagtccct tatctgaata aacggtgttc aaaagccagt ttctcggtat
    11281 taagccaaat ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttactactcg
    11341 aaactaagcg actttttcac aacctctggt acacggtgtt ccaaaagtag cttcttcgga
    11401 aataagttgg aatttcacaa aaattcaaga agtaattttc gtaaattcct tcttatttct
    11461 tgaagctgat tacttctgtc acaacctctt tttattattg agaatttggt cgaggaataa
    11521 tcgtttttgc catccaaata tccggtttgt cccagccatt tgcatttggt aagacaccta
    11581 cgattttata acctaatttt tcatagaatt catacggatg ttcacgaagg ttctggatgg
    11641 aagccacttt atcaaatgta tgctcataca ggtccgtttg acttaacgtt gttccatggt
    11701 ctaaatcatc cgtacctaaa taaatcgtga ttcctcctct ggaagctact tctttttcta
    11761 agtaattgac taatcgagta cctatttggt tctttcgtcg ggagctttct acaactaatg
    11821 gatgcaattc ccaacctgtg ataccgtatt gagggattgc accaataaat cctactaact
    11881 catcttggtc taccgctgct accgcgattc gttctggatt catcatttct tctacttctt
    11941 ctgccgagct gtctccatat tcttccggcc aagtcagtct cagtaaatca gaaagctgat
    12001 ctttcaatac tggattatta cggtcaaatt cactgattat catacgtctt cccccttgtt
    12061 cttgcttatt ctaacagtcc ttttgaaaaa acgaaatgtt ctcgctcaaa aatctttagc
    12121 taccaatcag tatctctatt ctttcattat ttatcttact tataaaataa aaaaattata
    12181 cagaaacact ttattcaaaa taggaaagat caggaaagcc atcaattgga ggaaaaaact
    12241 ctgtttgttc atcatgatat agattatctt ttgctttaaa aaaaaccaat tatgaattga
    12301 caaataaaac ctgttttcat ataataatct taatttttag aatttttaga taattacaaa
    12361 aaagcaaagg gaatcggtag aaatactaag aggaacaaaa attcattatt gtcgttttca
    12421 atgtttttgt acttgttcgt ttttaggata cttattgatg atttcaaggt gttcatgcca
    12481 ccttattcat cacttatcct gtcttaggtt cacctctact ccatctacag ccgcagtcag
    12541 atcgatcact ctttaaaatt ttaacccgtt tcaac
//
LOCUS       DACBZN010000084        12526 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.084, whole genome shotgun sequence.
ACCESSION   DACBZN010000084 DACBZN010000000
VERSION     DACBZN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.084"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(144..539)
                     /locus_tag="IVD64_002114"
     CDS             complement(144..539)
                     /locus_tag="IVD64_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6149354.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(557..1519)
                     /locus_tag="IVD64_002115"
     CDS             complement(557..1519)
                     /locus_tag="IVD64_002115"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP6149355.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(1519..1731)
                     /locus_tag="IVD64_002116"
     CDS             complement(1519..1731)
                     /locus_tag="IVD64_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP6149356.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(1752..2450)
                     /locus_tag="IVD64_002117"
     CDS             complement(1752..2450)
                     /locus_tag="IVD64_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP6149357.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(2470..3012)
                     /locus_tag="IVD64_002118"
     CDS             complement(2470..3012)
                     /locus_tag="IVD64_002118"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP6149358.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(3144..4148)
                     /locus_tag="IVD64_002119"
     CDS             complement(3144..4148)
                     /locus_tag="IVD64_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6149359.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(4145..5134)
                     /locus_tag="IVD64_002120"
     CDS             complement(4145..5134)
                     /locus_tag="IVD64_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6149360.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(5131..5937)
                     /locus_tag="IVD64_002121"
     CDS             complement(5131..5937)
                     /locus_tag="IVD64_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149361.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            6103..7059
                     /locus_tag="IVD64_002122"
     CDS             6103..7059
                     /locus_tag="IVD64_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6149362.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(7136..7654)
                     /locus_tag="IVD64_002123"
     CDS             complement(7136..7654)
                     /locus_tag="IVD64_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6149363.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(7742..7891)
                     /gene="rpmG"
                     /locus_tag="IVD64_002124"
     CDS             complement(7742..7891)
                     /gene="rpmG"
                     /locus_tag="IVD64_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6149364.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8119..8565
                     /locus_tag="IVD64_002125"
     CDS             8119..8565
                     /locus_tag="IVD64_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6149365.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(8760..10655)
                     /gene="asnB"
                     /locus_tag="IVD64_002126"
     CDS             complement(8760..10655)
                     /gene="asnB"
                     /locus_tag="IVD64_002126"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP6149366.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(10980..11954)
                     /locus_tag="IVD64_002127"
     CDS             complement(10980..11954)
                     /locus_tag="IVD64_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6149367.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         3968 a         2624 c         2109 g         3825 t
ORIGIN      
        1 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
       61 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
      121 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
      181 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
      241 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
      301 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
      361 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
      421 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
      481 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
      541 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
      601 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
      661 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
      721 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
      781 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
      841 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
      901 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
      961 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
     1021 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
     1081 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
     1141 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
     1201 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
     1261 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
     1321 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
     1381 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
     1441 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
     1501 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
     1561 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
     1621 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
     1681 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
     1741 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
     1801 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
     1861 tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc accgacatgt
     1921 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
     1981 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
     2041 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
     2101 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
     2161 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
     2221 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
     2281 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
     2341 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
     2401 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
     2461 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
     2521 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
     2581 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
     2641 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
     2701 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
     2761 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
     2821 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
     2881 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
     2941 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
     3001 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
     3061 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
     3121 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
     3181 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
     3241 cttagtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
     3301 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
     3361 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgccaaagca
     3421 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
     3481 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
     3541 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
     3601 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
     3661 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
     3721 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
     3781 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
     3841 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
     3901 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
     3961 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
     4021 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
     4081 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
     4141 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
     4201 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
     4261 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
     4321 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
     4381 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
     4441 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
     4501 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
     4561 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
     4621 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
     4681 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
     4741 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
     4801 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
     4861 agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa
     4921 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
     4981 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gcttttccca
     5041 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
     5101 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
     5161 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
     5221 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
     5281 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
     5341 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
     5401 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
     5461 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
     5521 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
     5581 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
     5641 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
     5701 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
     5761 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
     5821 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
     5881 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
     5941 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
     6001 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
     6061 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
     6121 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
     6181 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
     6241 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
     6301 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
     6361 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
     6421 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
     6481 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
     6541 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
     6601 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
     6661 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
     6721 aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
     6781 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
     6841 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
     6901 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
     6961 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
     7021 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
     7081 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
     7141 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
     7201 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
     7261 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
     7321 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
     7381 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
     7441 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
     7501 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtgtgttcgc
     7561 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
     7621 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
     7681 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
     7741 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
     7801 ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt tctttacaag
     7861 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
     7921 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
     7981 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
     8041 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt atgtttttta
     8101 ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc aatgaaaaac
     8161 ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt agatgaaggg
     8221 taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg gtatgaagat
     8281 gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa
     8341 cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt agaatatgag
     8401 gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca ttttgataca
     8461 aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc aactttgttc
     8521 atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg
     8581 ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt cctctgccaa
     8641 ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg aaattgtttc
     8701 ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt tgattttcat
     8761 caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac aaagtagact
     8821 ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc tttacgtcct
     8881 tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa
     8941 tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa ccaagctttg
     9001 attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag atgtttgttc
     9061 gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt acttggtatt
     9121 tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc caaagaactt
     9181 gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg atgaagatcg
     9241 acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta atgaacagtc
     9301 atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc tgatgcttct
     9361 gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc aagtgattca
     9421 tataaatgga tccgtccatg gaaatttttc atttttccga ttttacgtcc taaattatag
     9481 cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa ttttgaattc
     9541 gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc tccagattgg
     9601 actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac gcaagaagga
     9661 tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa cgacatgtct
     9721 ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt tctggcttca
     9781 atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc tggtttcaat
     9841 acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac ttctacatca
     9901 ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc gatccattct
     9961 tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg aatgtctatt
    10021 tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc tttgaagaat
    10081 gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc ttctttgttc
    10141 aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc aaacataaat
    10201 gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct tgcaccgaat
    10261 aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat cttttgcaat
    10321 aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga
    10381 aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata gatttcgcca
    10441 ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc gccctctaag
    10501 tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa
    10561 ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt ttttttgttg
    10621 tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc taatataaaa
    10681 ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac gaatattttc
    10741 tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt acagaaaata
    10801 aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt
    10861 taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa
    10921 gatcgtgtct aattatgact gtatcagaaa atatgatacc aaacggatct attcattaat
    10981 taattgacat agtttatctg ttgctctttt atgactggat aactgaccaa ttcatgacga
    11041 tccaagtctt ctttattcat atttacagca gtaatttgac tgtcttcata cgtacgataa
    11101 taataaatcc ctttatcaac gttacagcaa gaagaataaa tcgtatattc gtattttctt
    11161 tctccagtat cgcatactcc tttttgttgc tcgactgagc ctagtatatg aaaaaattga
    11221 ctgatacttt cagattctga atcacctgat gcagcattca ttttagtaaa tgtggctttt
    11281 acaaatcgag aaacagaaga gagatctcca ggtaagccca atcctcccat cccacgacta
    11341 taaacattca agctgatctg gtttgaaaaa tgattttctg gtgttccatt tgatagcgag
    11401 cgataattgt tcagattaaa taattgataa tcaaatggcg gattatttgt caacacaccg
    11461 acaggattat catataaatg aagtccatct ttcatacttt caattactat cgatttttct
    11521 ttatcagcta ataaccagtg taaaggtgac aagggtagtt tttcactaaa atcgatattg
    11581 actagattga tagtagccaa taattcttta gcttcgtcta tggttgagca ttgccctagt
    11641 atccatggaa taaactcaaa tggtgcaacg ttatcttttc cttcttgtaa ttctttataa
    11701 tcagcattgc caggaaaatt cagtcctgcc atactcaacc ctttttcatt tgtagcctca
    11761 taatagagtg gatagttgga taccccagct gctattccta tcattgcata atgtttatct
    11821 aaatttttta cctttctaaa atcgaaacga taatttcttg gagtaatagt gaccacttca
    11881 ttataagaca tctcataatc aaaattcctt ccaaaataat ggtctttcgt tgcataagtg
    11941 attgcggtac acatcgtttc ccgtcctaaa acaaatcgtt tttacacttt tatcttacat
    12001 ctagtatttt ttttatcaaa aaaaggccaa agaagattga tttactagca tttataaaaa
    12061 atagtactca gggggaatca aaaaatattg taggttaaaa gtacaacaat aaggggctgt
    12121 gacaaaagtc ttgtcacagc cccttattcc gaacaaacgg tgttcaaaag cttgcatctg
    12181 cggtaataag cccaatcaaa cgaaaaaata ggaaaagcta tttttgtttg gccgttctta
    12241 ttacttgatg caggacagct tcttcacaac ctctaatcgt tgatataagg aaggtacagg
    12301 atttgaacct gcgcgccggt attagccggt tcgccggatt tcgagaatag ggtttaaagc
    12361 tgattttaag tgtttttcgc ttattatcag ctttttatta tctaaaaaaa caacttttag
    12421 agacatccat atactgcttg ggggagtcaa aaggggagtc atttttatat tttattcgca
    12481 atttaaatca cttagagtat gtacactgta ggactcgagc ctacga
//
LOCUS       DACBZN010000085        12301 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.085, whole genome shotgun sequence.
ACCESSION   DACBZN010000085 DACBZN010000000
VERSION     DACBZN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.085"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            31..1062
                     /locus_tag="IVD64_002128"
     CDS             31..1062
                     /locus_tag="IVD64_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6149368.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            1059..1826
                     /locus_tag="IVD64_002129"
     CDS             1059..1826
                     /locus_tag="IVD64_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6149369.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1926..3494
                     /gene="liaX"
                     /locus_tag="IVD64_002130"
     CDS             1926..3494
                     /gene="liaX"
                     /locus_tag="IVD64_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP6149370.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3794..4111
                     /locus_tag="IVD64_002131"
     CDS             3794..4111
                     /locus_tag="IVD64_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP6149371.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            4111..4461
                     /locus_tag="IVD64_002132"
     CDS             4111..4461
                     /locus_tag="IVD64_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6149372.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4628..5566
                     /locus_tag="IVD64_002133"
     CDS             4628..5566
                     /locus_tag="IVD64_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP6149373.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5581..6411
                     /locus_tag="IVD64_002134"
     CDS             5581..6411
                     /locus_tag="IVD64_002134"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP6149374.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6455..7480
                     /locus_tag="IVD64_002135"
     CDS             6455..7480
                     /locus_tag="IVD64_002135"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6149375.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7495..8433
                     /gene="galU"
                     /locus_tag="IVD64_002136"
     CDS             7495..8433
                     /gene="galU"
                     /locus_tag="IVD64_002136"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6149376.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8558..8998
                     /locus_tag="IVD64_002137"
     CDS             8558..8998
                     /locus_tag="IVD64_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP6149377.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            9001..9603
                     /locus_tag="IVD64_002138"
     CDS             9001..9603
                     /locus_tag="IVD64_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6149378.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9662..10768)
                     /locus_tag="IVD64_002139"
     CDS             complement(9662..10768)
                     /locus_tag="IVD64_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6149379.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            10978..11979
                     /gene="ccpA"
                     /locus_tag="IVD64_002140"
     CDS             10978..11979
                     /gene="ccpA"
                     /locus_tag="IVD64_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP6149380.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         4088 a         2118 c         2595 g         3500 t
ORIGIN      
        1 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaattagcat catcactata
       61 ttttttattt gtgtgcttac ttcatgggtt attcagcatc aagccgcttt atatgaagta
      121 cctgtcatcg agatagaaaa agtacgaata aaagaggatc atcaattgat tacaggaaaa
      181 ctgatgaaca cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat
      241 caaatagaaa cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat
      301 gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc
      361 attggcgcca tgttgcttat tggtggaaaa cagggtctat ttgctttagg aagtctcatc
      421 atcaatatcg ttttactttt acttttgttg ctcatcttta ctaaaacaga agggatttct
      481 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
      541 gatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
      601 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
      661 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
      721 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
      781 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
      841 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
      901 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
      961 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
     1021 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
     1081 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
     1141 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
     1201 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
     1261 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
     1321 tttgtttcct actattgctt cctatcactg attttttagc aatccaaggt tttacatcgg
     1381 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
     1441 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgcgagt gtagcaattt
     1501 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
     1561 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
     1621 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
     1681 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
     1741 tgtccgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
     1801 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
     1861 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
     1921 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
     1981 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaatcaaaa
     2041 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
     2101 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
     2161 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
     2221 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
     2281 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
     2341 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
     2401 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
     2461 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
     2521 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
     2581 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
     2641 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
     2701 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
     2761 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
     2821 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
     2881 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
     2941 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
     3001 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
     3061 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
     3121 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
     3181 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
     3241 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
     3301 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
     3361 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
     3421 cagatggtga aatcgcgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
     3481 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
     3541 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag tctatctctt
     3601 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
     3661 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
     3721 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
     3781 gaggtgagac atgatgaaaa aaaagttgac aaaatctaat aaaaatgttg ttttgacagg
     3841 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
     3901 tgtattcctg agcttagttg cttttggaag tccaattatt ttatacattt tattagcttt
     3961 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
     4021 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
     4081 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
     4141 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
     4201 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
     4261 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
     4321 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
     4381 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
     4441 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
     4501 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
     4561 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
     4621 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
     4681 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
     4741 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
     4801 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
     4861 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cagctccaga
     4921 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
     4981 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
     5041 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
     5101 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggccatcgat tgatcgcaga
     5161 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
     5221 cctggaacat ctgattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
     5281 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
     5341 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
     5401 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
     5461 agtagccgcg atgaatttcc gtgcacgtac aatgggcttt gacgcaacga aaacatttga
     5521 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
     5581 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
     5641 gcattgatca tcgtatcagg tatcattatc gctatgtggc ttagcagcag agaggctgtt
     5701 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
     5761 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
     5821 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
     5881 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
     5941 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
     6001 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
     6061 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
     6121 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
     6181 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
     6241 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
     6301 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
     6361 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
     6421 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
     6481 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
     6541 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
     6601 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
     6661 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
     6721 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
     6781 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
     6841 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag caaaacatga
     6901 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
     6961 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
     7021 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
     7081 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
     7141 agcaatgggt gccaacccgc tgacctttat cggattaagc ggaataggcg atctagtcgt
     7201 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
     7261 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
     7321 agcagcgatg gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta
     7381 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
     7441 tggaaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
     7501 gttaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
     7561 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
     7621 gaagcgttga aatcaggtat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
     7681 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
     7741 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
     7801 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
     7861 ccatttgtcg ttatgctagg cgatgatata atggaagacg aagtaccatt gacacagcaa
     7921 ttaatcgatg attacgaacg tactcatgct tctacgattg ccgttatgca agttcctcat
     7981 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
     8041 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
     8101 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
     8161 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
     8221 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
     8281 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtacatc
     8341 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
     8401 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
     8461 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
     8521 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
     8581 attgatcgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
     8641 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
     8701 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttac tggtcaaaag
     8761 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
     8821 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
     8881 gaaagtcggc ggaatcggta aaacgacagc taaagcgacg gttgccggaa aagtcggtgg
     8941 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
     9001 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggcacagc cgcagcaatc
     9061 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
     9121 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
     9181 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
     9241 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
     9301 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
     9361 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
     9421 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
     9481 atgtcagaag caaaaccagt tgcagatgac tcaaaagaaa tgatgaaaat ggcaaaagaa
     9541 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
     9601 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
     9661 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
     9721 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
     9781 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
     9841 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
     9901 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
     9961 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtag attttttctt
    10021 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    10081 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgta tttcctggtg
    10141 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    10201 aaacaccttg ttttttcaat tggtattcaa tttctgcaat gatcgcttgt tcagtcacgc
    10261 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    10321 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    10381 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    10441 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    10501 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    10561 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    10621 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    10681 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    10741 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    10801 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    10861 aattttcatg aaaacagaga taaaacggtt gcaattcttg agattatttg atattctatt
    10921 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
    10981 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
    11041 gtttcacgtg tcgtaaatgg caatccaaac gttaaaccag caacaagaaa aaaagtcttg
    11101 gaagttatcg atcgcttgga ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag
    11161 aaaacaacaa cagttggtgt aatcatccct gatgtcagca atatgttctt tgcttcttta
    11221 gcacgtggta tcgatgatgt ggcaacaatg tacaaataca atattatctt agcaaattca
    11281 gatggcaacg accaaaaaga agtcaatgta ttgaataact tactggctaa gcaagtggac
    11341 ggcgtgatct tcatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
    11401 aaaactcctg ttgtattagc tggctctatt gatccagatg aacaagtagg tagcgtaaac
    11461 attgattatg tatccgcaac aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa
    11521 aaaatcgctt ttgtcagtgg tgcgttgatc gatcccatca atggacaaaa tcgtctgaaa
    11581 ggctataaag aagcattaag cgaaaataac ctaccatata gcgaaggatt gatctttgaa
    11641 gcacaataca acttcaaaga tggtctatct ctagtcgatc ggatccataa tagcggtgca
    11701 acagctgtct atgtttcaga tgatgaatta gccatcggta ttttggatgg tttattagac
    11761 cgtggcgtga aagtaccgga agaattcgaa atcatcacaa gtaacaattc actactgaca
    11821 gaagtagctc gtcctcgttt aacaagtatc acacaaccat tatacgattt aggggcggta
    11881 tctatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
    11941 ttgccttatg gaatcgaaga aaaaggatct acaaaataat aaaaggacaa ccattcagag
    12001 gttgtgtgcc tgccgtttaa ctctgcgtta caaggagcaa tttcaaaaaa cagcatctca
    12061 tgtttgatga atattgtgac tcgtgattcg gagttggcag gagaacaccg tttatttggg
    12121 aaaaggggtt atgacaaaat tttgttataa tccttttttt gtgtcttaaa aaagagagaa
    12181 agtggttgaa attacattta tcgtatataa aacaatccgt gtatagatgc tgcattttgt
    12241 tcaccaccat caaaaaaata agggactgtg acaatactat tgtcacaatc ccttattact
    12301 g
//
LOCUS       DACBZN010000086        12021 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.086, whole genome shotgun sequence.
ACCESSION   DACBZN010000086 DACBZN010000000
VERSION     DACBZN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.086"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(1..209)
                     /locus_tag="IVD64_002141"
                     /pseudo
     CDS             complement(1..209)
                     /locus_tag="IVD64_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            complement(340..2217)
                     /gene="ltrA"
                     /locus_tag="IVD64_002142"
     CDS             complement(340..2217)
                     /gene="ltrA"
                     /locus_tag="IVD64_002142"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6149381.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            complement(2905..4599)
                     /locus_tag="IVD64_002143"
     CDS             complement(2905..4599)
                     /locus_tag="IVD64_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP6149382.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(4762..5355)
                     /locus_tag="IVD64_002144"
     CDS             complement(4762..5355)
                     /locus_tag="IVD64_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149383.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(5356..5688)
                     /locus_tag="IVD64_002145"
     CDS             complement(5356..5688)
                     /locus_tag="IVD64_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149384.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFGAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(5738..8110)
                     /locus_tag="IVD64_002146"
     CDS             complement(5738..8110)
                     /locus_tag="IVD64_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149385.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEIKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQ
                     FKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQNIVTREAEP
                     KPIQNVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLRDIKQDIKP"
     gene            complement(8107..10716)
                     /locus_tag="IVD64_002147"
     CDS             complement(8107..10716)
                     /locus_tag="IVD64_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP6149386.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            complement(10716..>12021)
                     /locus_tag="IVD64_002148"
     CDS             complement(10716..>12021)
                     /locus_tag="IVD64_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149387.1"
                     /translation="FDYILDQYPPEHEWMKEKNKLFLNELYSKYQLPQTYQAFLESKE
                     ELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEERQQETDQEINEYATEQSSNWEK
                     AKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVAKINNFTQTNQEALSKEKENLL
                     KERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTTTEVLMEKYEKIQQELQVQIPK
                     WKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQDLAEQKMAIEKQRLEQEKEKEA
                     AARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTPSPQRSFNGWMVGC
                     IASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQEQARKEYEQQLEELLNRTN
                     QSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNEVK"
BASE COUNT         3177 a         2468 c         1925 g         4451 t
ORIGIN      
        1 aaaatagatt catccatacg cataaagatc ttcgtagaag aaaattcaaa gactttcttc
       61 agtttcgaga agttatcaga agacatattt tctggcacca tttcttgtgg ggattgtgta
      121 gctaaaacag tcattgcttc tactttacgc atttctcgtt gaaagtttgt gacataattt
      181 accgtttcaa taaaatctgg attaatcgat actgataggt aagagtttga tattatctcc
      241 gtcttttccc ccagtccaca ccgtacgtgc gactttcacc gcatacggcg ttccctcgtc
      301 tataaaagac attctgtttt cccgtcctac aaatatttat taagatattt cttcgttacc
      361 aacttttaat ctcaactcat tgagtttttc tttttgtttt ggatttaatt ttagatattt
      421 tagaagttcc tcaatcgttt cactttgtgt agcgtgtatt acccgatgaa cctcctcagt
      481 aacaatcact aggttttcat agctatcact tttgctttca tgataaggga taatatgatg
      541 acaatgcatt tcagataaga taagctcctc acctgttact gcacatcttc ccttttgagc
      601 gatgaatttt gaaattcgat tgtccgcaaa ttcaatcgtt gctctcttac taagcactgg
      661 attgctacac aaccacgata tcttccattc ttctacggtt tgttgcttct tatgaatggc
      721 ttccctacct tcttcggtgt atttattaat atttcttttt ttacacatcg gatgtctgtg
      781 ttgaatatac cctactggta atattggaaa ccctcttata tatcgtattt gtcctgaccg
      841 taggtaaggc ttgtatcctt cgtactttcc gtattgtcct tcttttgaga ttccttgttc
      901 tcgtagtcgt atttcaaaac ttggtctgag ttctcttgca atttttcgca ttgctacact
      961 acaatgagtc gcaattttga agtaattatg aatcccaata accatactgt tatagactcc
     1021 aatatagata aaggtatctt tgctattagg acttttttga attcgtttga tttgtttttt
     1081 tagtttcact ttaatcatgc tttgtgcttt tgtacctata tgcgtattaa caaccttttt
     1141 gttacctttg gtcactaatt tcatcgtaaa gcctaaaaat tcactttctt cttttgaaag
     1201 atttgtgatt tttgactttt cttcagatat tggtaacttt agacgctcct gtaaccacat
     1261 tttagttgca tagaaaactt ttttcgcttc attataattt ctacagaaaa ttttaaaatc
     1321 atctgcgtat ctcactaggt atatttcttt tagagttgta ctctttctga gcttgtgata
     1381 gatattacct ttatcccaag ctccattttt cctaaaatga tgttttagct ctttggcatc
     1441 tttttcctcc cactgatttg ctatccacca gtcaaattcg ttgagattga tgttcgcaag
     1501 taatgggcta agtattccgc cttgtggtgt tccttttgtt gggtatatca acttcccatt
     1561 tggaagtact acgggtgctc ttaacatttt cctaatgatt aagagaagct gtttgtctcg
     1621 tatacccata gaccagattt gtctcatgag tttagtatga ttgacttcgt cgaagaaacc
     1681 tttgatatct acatctacca catattccat gtgactttga ttcattcggg ccatacaatc
     1741 tgctattgcg tgttctgctg atctatttgg gcggaaacca tagctgtgat tattgaattt
     1801 tgcttcacat attggttcta atacttgtaa gatgcattgc tgcgcgattc tatcccatat
     1861 tgatggaata cccaacggtc ttaatttacc attatattta ggaatttcta cacgccttac
     1921 tttccgaggg ttataatgtg cgaatcgctt cctaacaatt tctacgaatt cattacggtt
     1981 gagattttca atatctttaa tcgttactcc atcttctcct cttgtcacac taccgtgatt
     2041 tctcttgata ttacgatatg ctaggacaat gttgttatca gaaatgataa tctgcattag
     2101 attcttgaaa ttatctccct tcaaactttt ggagtatatc ttatcgaaga tttccgtcat
     2161 gccataatat tctgcatgcc gcaacttcct atcttttgga cttgtttctt ttgtcatggt
     2221 ttatcacgct ccaattggct cgtgaccttt ttagtcatac gagaatccca tggttgaacg
     2281 ggtttcatga cttttacaga cgaatcgagc ctgttcctcc agttccatta cagaaccttc
     2341 atcagtcgtg actctacttt caccatcact aaagtaagtt atttgtcatt cacatatttc
     2401 ttactaccac ttcatcgagt atgcactctc catgttgatg gcttcccctg ttcccttata
     2461 tctatccaca tgccttaact tagatgctag cttttgcccc ctaacttctc ctacgcctgt
     2521 aacgcagcat ggtctttcat aacaacgact tttactcaac cacaagttag cacaatgagt
     2581 gattttttgc tcactgtctc tcccatttct aggagctatg cctttcggac agtacattcg
     2641 ctagttcgtc agtacgtttg tacatactca ccatattaag tttttttgga cacccgactt
     2701 atcggataca ccctttacct ctagagaact ttcgtggtat tcactatttc ttttacctta
     2761 cggtatcctt cagccgactt caccgagctt cacacaacgt tggtctgcat aaactacttg
     2821 ttgcatgtcg gagtatcagt ggaagccctt tgtggcgtta cccactcatt tgacttctcg
     2881 ttataagagt tattacaata gtttttagac tacttgcaac ccacagcttt tcactgcaga
     2941 acaggtcgca ccattatggc actcatcaat catcgccaag aagcgtttga agtcttgttc
     3001 ttccatttgt ttttctttaa gtaaacgtcg ttgtcttctt ccttgaatca atgcctgatt
     3061 ccaaatcaac gtcatagcca tgtacatttg acattgttgt acccctcgat ctaagcctgt
     3121 aatagagtca atgtcaaaaa agaccaactg ttgttggtca aggtgtcgaa tcgtactgtg
     3181 accatcaaaa atcatgccat acgtctcaca taaggattct acgatggatt ccatttcttc
     3241 ttttcgattc tttttctctt gggaagcacc tggtaattcc agttgtccta aaaagtctaa
     3301 aaattctttg aacgtcggat aagcttctgg atctttccct gtgacttgcg tctcagtatt
     3361 tgaacgcgaa tttgaggaag taaagccgcg tggtactgtt ccatactctt cataaaaacg
     3421 atttaagtag gtcttaaatt ctgctttcat cgttcctttt agctgaggac tcactaactg
     3481 tacctgatag actactttgt ctaagtgtgt ttgataggac gctgcttcat tgatcttcaa
     3541 atctttgact gatccatcgt catataaact ggatgcttca tcatacgtcc gtgtggcaaa
     3601 tatttctaaa ggattgatca tcccgtcaga cccgtctaag cgcaccataa tgccgttata
     3661 ttcctttaag aaggggataa agtctttccc cttttcaaag ccccaaataa agtctcctgc
     3721 agctaaatgg gcttccgcga tcattttaag aacggttgat ttaccagctc ccatcattcc
     3781 tagaagcaag gtattagtag acaaccgcac actattttta tatgtcgcat tccagacaaa
     3841 tgggccacct gtcatggttt ccccatattg agtccctcct ggatctttta gcgtttggtg
     3901 gtggaacggg aatcctccgc caatatttaa cgatggtagt gattgccctt tgatatttcc
     3961 aaaccattca gcttgttcat cgaacatggt aaacatcgat tgccaatctc ttttcgtttt
     4021 gtgcagatag gtcactgcac gataatccat cccttttaaa ttttcaatca gttcctgcgc
     4081 tcgttcatct acttcttgaa tcgtgtttcc gtaaacatgt actctcactt tgacttgctt
     4141 agaaatttcc ccattagaag tcaatttacg agcatattcc gctagttcca ggtattcttc
     4201 tgcgctttta tttcggtcag tcgcttttcg ttcattatct gatcgatcat ttaattcatc
     4261 taaggcacga ttgatttgat caatcacttt ttgcttttca tctgtgctaa catcgagtgt
     4321 ggccactgta tgaggattgt tcatgagatt ggccaaccaa aaaagcgtag gattttttgt
     4381 gtagtgataa actgtcacac atcgtgaata accattacca tacgtcacat attcttcatc
     4441 aaaatttaac ccaccttgtg gcatcacttg ttttaaaaaa tctaaatcat accctttgga
     4501 tttcaaaatt tctatttccc gatttcctag ttgctttaat ggatcaagta ctttttgatc
     4561 attttgtggt gaaaaagctg ttttaattgc ttctaacatg tacagtcacc tcttcttgtt
     4621 tgattcttta cttggttaaa agattgccct tttcccaact tacccaggct taaagtgtga
     4681 ggaaattttc tagtggtctc ttccttcgga acatcttttt ctaaccatat caatggatat
     4741 ttcattctct cttctccatt cctactcatc attcatattg ttcattcgat acagaatctt
     4801 gatcaattct tttttgtaga gtggttttaa ccctaaaatg ccactagatc gcattaactg
     4861 acggtttctg gtttgtaaat ccttgattgt ctttccttca atgacgaaaa agaagcttaa
     4921 gtcaggacgt tcttgttctg ctcgtttcaa ttcctggatt tcttcgacta atttcttttc
     4981 atattttttt aatcgttgta gatttcttcc taaggattgt tccatacgat tcttatctaa
     5041 taactgccgc ttttgcacga tttgttccgt tagatccgtc tctgtagtca aactaactaa
     5101 cttaattgtt ggcacatacg tacgcatgac ggtggtcaac tgattcattc gttccagttt
     5161 ctcttgttca tttaatccct ccacgtcagt ggaaacgaca cgataataac gttgaaaatg
     5221 atcttcttta gtaactaaga ctccctcttc tgtacaggat ttataaggaa ataaatccaa
     5281 gatagatcgt tcttctggtt ttccaaactg caaaatagat ttcttatcaa ctgaattttg
     5341 tcctttgact cgcatttatt catcctcctt atacgtacca tgataatttc ggttaggatc
     5401 taacgcatga taatggttct tgtccgcaaa aaaaatagac tcgataatcg caaaaatttg
     5461 tcgttgacct gggttattat agggtcgcca aacaatcact aacgctatcg caatccataa
     5521 gccataattc aagagtgcaa aaaagagtgg taagtggaca agattattga ccattttcgc
     5581 cccaaaaaag cagccaccaa gcgttaatag atctaaaaag aaaaaagcga aaaacttcca
     5641 ttctccacta aatcttggag ggtttttcat gtttatatat ttatccataa ttgatttctc
     5701 cttttcatat aaagaaaagg cctaacttgt aggcctttta tggtttgata tcctgtttga
     5761 tatccctcaa tagttgttct ttcaatgcat tgttcctgtt tttggtatct cttgtcacat
     5821 ttatttttgt cggtgcagta aatgccacat ttgctggctt gtaacctgta tgaacgtttt
     5881 gaataggttt tggttcagct tctcttgtca caatattttg aacggacgca ttctctgtct
     5941 caccactttc agataaattg taagaaacag ttgtcaaatt cccattttca ggcaaacgac
     6001 ttgatttcat ttgaacagta cctgcaggtg attgatgaac aaagctttga tcattctctt
     6061 tgaattgatt caaacgacta tctttcattg gtaaattttg tgtcttaggc aaaccaattt
     6121 tcgttgattt ttcagttcca acttcgatgc cattatcatt tggcagatcg atggaatcat
     6181 catccgtttg tacttcttga tttcgttttg gtaaatctga aatgcgcttt tcattttcag
     6241 aattaatgtt agatttataa cgatattgtt ctggatctgt ttttggtaat tgaccatttt
     6301 gtccttcttc tgaattttcg ttagaatgca actcatcttg gtgatctttg gctgaaggaa
     6361 tcgctgcacc ttcttgattt tctttttcgg ttgttgtatt agcttttact gctgcattga
     6421 cttgtccttc accatacgca tcttttagtc cttctttgat ttctgaagct ttttcttttt
     6481 gtttttctag tgtttcggat ggattcttca cagcttcagc caattcttta gctctcgtgc
     6541 cgtctaattt ctcttctaag gtgtctttgg ctttttctgt aagatcttca gctgttcctt
     6601 ttaacccacg ttcattgacg taaccaagac cttctgcggc tttagaaaca ccatttttcg
     6661 ctttcccgaa catttcggaa actgttttct tactattagg tacagttgta gctttatcgt
     6721 ctgattgttc ttccccgtta tcaggggtaa catcttttcg attaccaaat ccatttttac
     6781 gaatatcatt taaattgctt aaaccactct taccagcact tgcaaggttt ttagccccgc
     6841 tacccacatt tttcgcaaca tttcctaagg caaacatgct aagaagagaa ttgctgccgt
     6901 ttttcagact cgtatcaacg ccgaaaattt tcgtaactga ttgagagcct ttgaatagcg
     6961 caatggttaa tacaattaaa gcgacaacat acaagaatcc attgatcttt cctgccgcat
     7021 gcaaatcacc gatacctgaa agcattaatg tatagattcg taattctaaa atcgtaaatg
     7081 ccaataacaa gaaggattga gaaatatctt ctatagcact tttcatccgt tgtcctgtct
     7141 caaagtccgt tgacgcggca atcactaaat agaatttttt aaataccagc tcaataatgc
     7201 aagtaacaat cgtaaacaag ataaataaat aggctaccga caaagaagat tcaccagcta
     7261 aaataatacc tggactacta atgtatcggc gatagcctga agtatacaca tattttgcta
     7321 aaccttcatc actaatttga acagggattt ctttcccatt atcgtctaac tctagtcgat
     7381 actgtaatgc ctttaaccga tctttttgta ctgtactaat atcacttgat tcagacattt
     7441 tcttagcttg atcaggtgta ataatcccat tgatattcgc tgttttaaaa tttttcgccg
     7501 tgagagcatt tcttttgtct gttggtatgt gttctacttt gttcggatca ttttctagta
     7561 acgtactcac atcaatcaca ttattttgaa taatttgaaa agcaggtgaa ccagttttat
     7621 ctgctttatt tgctgcagtc acgtctccgt aaaagttttt actaatattt agcccttcat
     7681 cgattaaaga actaccacca aaaatcaaaa tcatggccat aaatagatta accaagatat
     7741 ttttgatttt tggtggatgt ttactcataa aaaattttaa gcctaaataa attaatgtga
     7801 cgacacaaaa aagagccaat accttactaa tcaaactttg aaacagttca ttaacaccag
     7861 cggcgttcaa taagtctttt aaatcaaagc tttgataaat tgttttttct agtaaagtat
     7921 tgagattata taacccttta atcaaacccc ataaaaaagt attacaaatc caactgaata
     7981 catcttgata ctgcagataa ttaccataat cttgtaagac tttcgttacg gttttgggat
     8041 ctgcatcccc atccccaaat aagcctgacc accagtcggc aatgccggaa aatattcctc
     8101 caatgatcat tctttcaccg ccttttcagc ttctagcaaa agtcgtttcc ttataacctc
     8161 tgtaatttct tcattttgac gcaatttttc aaggtattct tgcaaacgat ctatttgctt
     8221 ctcttcataa aattgaataa gtaacttttg tgccgttgag ttgtctaaag attgaacgat
     8281 tttaataaaa atgttttctt tagcttcctc ttctatttga tcgtgcatat tgtgataaag
     8341 tcgacgtgca tagatatcag gtagaaaata atcgtaatca atttgtaaag ccttaagatc
     8401 caagtccgca tgtgtacaat gtatgtctat atcattgata tcccacgatg gattgaaatc
     8461 ttcaatatat tgataagcat aaggaagatt cgtttcccca tgattaaaaa taggatacgg
     8521 ccgtatttta tgacctttca aatcttgtcg ttttaaagga gcgagtacaa tcgtttcacc
     8581 ttctaaaaaa gtagaaattc tttcaggagt taagatgcga tctgaatcaa cattgcgatt
     8641 gatatgagta ttcataccta atgaattgat ggtactgttc tcttgttcaa ccgtcttatt
     8701 ccccactttt ttggaaaatt cttctatggt attatcgtct gtcgacataa tataaatatg
     8761 tgtctgacaa ttttctttga taatcgctgc gttttgttcc ccatacttcg cataaagttg
     8821 ctgataggat tggataaaca aatcccacaa catgtttcta ccagctgtga ctgtcatgat
     8881 tccttccata ttatccattg gtggcatatt cccaaattca tctaaaataa aatgaatcct
     8941 acggtgacat ttatctcctt tagtcttaac acattgcgta gataattctt tatacaactg
     9001 actaagaaaa atactaacaa tgatatggtt agatggatca taatcaggtg tgagcataaa
     9061 aatagccatt ggtttatcgg tatagtgcat ccgaatagac tctactaata agttactttt
     9121 atcctcatct aatgttagaa cggcttcttt ttctaattct ggcaaatggg tttctcctcg
     9181 tttttttcga taaacgatat ttccctcttc gtctttcaat actctttgag aaaactcata
     9241 ccaagcaaca tattcttttc ctcgatgatc gtattttaag caaacatgat ctccctgttc
     9301 taaatgacaa tcaaagttaa tttcacaaaa acctctatag cctactttga cacgttcctc
     9361 atgaaatttt tttccttgat gataaaactc tacaacaatt cgctggttta acagactctc
     9421 gtgtagtttg agatccaaat acttcggaaa accaatggac ttataatcaa tggttgattg
     9481 agaagtcatt ttcgcaattt tcgtcagtgt aaatggattt agtcctgtga tcgctgtcga
     9541 tagaatgttg cctttcgctt tatcagaacc aatttttgtt gccccatatt gtttttttgc
     9601 tatgtttcca ggtggtaact gattcataaa ttcatctaaa gcattgagct gcttgaattc
     9661 attatcagga tcttggtaat taaaaccacc aatctcagaa agaagttcga cgagattatt
     9721 cggtgttaat ttggagtaat cccctgtctg atccgcttgt tccaataacg caaaaataag
     9781 cgcattgaca gctttttgcg cattttcgtt aaagtattta ttgtctgaag cgttggcatc
     9841 ataataaatc gtattcgtga aagtattggt taactgtgtc gctgtgtcta tgtctcctcg
     9901 cttccagtat ttcacgatga gtgccaatgg attatcactc attgattgca gtggatcggt
     9961 gatattaaat gcatacacat cataccctct agcttctagt tcatctttac tagcaacata
    10021 taattctgtt tttggatcat taacaaccat tgaactttgt ctttctgcac gagacaaatt
    10081 gtcaatggtt gctaaaactt ctatctgtcc tttccctgaa cgggaagtac caacaataca
    10141 agtatttact gtatccgtct gaatatacca gtacttttta taatgagtaa ttggtattcc
    10201 gccataacca ggaaaacctc cctttgattc tggtatttgc ggataaattt gttggatttc
    10261 ctttaacgtt tttaatcggt tatctccttt ttgcccatct gctagaggac gaaaccacat
    10321 gtccattttc aatcccataa gtacagctgg aacaacacaa atcgtagaaa caactaaacg
    10381 tgtgcctaaa cttgttgttg gggtcaaagg attccaaaaa ccagttgaat caaaatggaa
    10441 taactgcggc atttcttgta actgaaaaat attcactcct tgtaaacttc ctggaataat
    10501 ctcaaatcga tgaaatgctt ctggtattct tgtaacaaca tagtttaaag gaagataagc
    10561 taagccacta taaaaaatct tttgagaaat acgtttgtca tgtttttctg gtagctcttc
    10621 tcctgaagga gtatagcgat agaaatgaga aagataccta ttcttttcta aattgaatcg
    10681 tttgtctttt cgtctcgttg tttctctaac acgcattatt tcacctcatt tgattctgaa
    10741 ctttcggaag tgtttgattg tacagttgaa tcgcttgatc cttctttagc cttactagga
    10801 tcactctctt tgctattttc catacttgat tctgttgttg actgattggt acgattcaat
    10861 aattcttcta attgttgttc atactctttt cttgcttgtt cttgggcttc ttccttgatt
    10921 gacgtcgttg tggccacaga atcagcctgt gaagattgat tgatatgatt aaaagcaaat
    10981 actgttcctc cacctagaag caagcttaaa cttgcaatac atcccaccat ccatccgtta
    11041 aaggaacgct gtggtgaagg agttagattt gtttggttgt tcattggggt ttgaacagca
    11101 gctactgaag gcgttggttg aggttgtgct agataattta actgagcttg tcgcaatgta
    11161 tccatttgtt cttgacgttc cttacgagca gctgcttctt tttccttttc ttgctctaac
    11221 cgttgttttt ctatcgccat tttttgttcc gcaagatcct gttcccgttt tcttatttct
    11281 aattgttcac gctctttttc tttttgccgt tgtttttctt cttgttcttt caattcttgt
    11341 tgttgaattt catactcttt tttccatttt ggaatttgca cttgtaattc ttgctggatc
    11401 ttttcgtatt tttccataag aacttcagtc gttgtttgat atagcttatt cagttgttca
    11461 tttaattctt tgctaatctt tagcttatct gtacgcaaac ctcggtaaaa ttcttctttt
    11521 cgttctttaa tacgttcttt taacaagttt tctttttctt tcgataacgc ttcttgattc
    11581 gtctgagtaa aattattgat ctttgctaca cacaaggaat acgcattgtc gcgtttcttt
    11641 tcgtatcgct ctttaatttt ttgtacttct tcttcaatca ctcgctgttc ttctttattt
    11701 agttcctctt ttgctttctc ccaattagac gactgttccg tcgcatactc attaatttct
    11761 tgatctgttt cttgttgacg ttcttcaaag gtggctaata aatcttcttg tgcaagctca
    11821 tgccaatttg ttgattggac agactctaac tgttctaaaa gtgtgccttt accctgtgag
    11881 attaattctt ctttactctc taaaaatgct tgataggttt ggggtaattg atatttcgaa
    11941 taaagctcat ttaaaaataa tttgtttttt tctttcatcc attcatgctc tggcggatat
    12001 tgatccaaaa tataatcaaa t
//
LOCUS       DACBZN010000087        12000 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.087, whole genome shotgun sequence.
ACCESSION   DACBZN010000087 DACBZN010000000
VERSION     DACBZN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.087"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVD64_002149"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVD64_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP6149388.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVD64_002150"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVD64_002150"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP6149389.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IVD64_002151"
     CDS             complement(2169..2906)
                     /locus_tag="IVD64_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6149390.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IVD64_002152"
     CDS             complement(2997..3524)
                     /locus_tag="IVD64_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP6149391.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IVD64_002153"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IVD64_002153"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP6149392.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IVD64_002154"
     CDS             complement(4401..4886)
                     /locus_tag="IVD64_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP6149393.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IVD64_002155"
     CDS             complement(5039..5236)
                     /locus_tag="IVD64_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149394.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVD64_002156"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVD64_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP6149395.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVD64_002157"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVD64_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP6149396.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IVD64_002158"
     CDS             9211..9423
                     /locus_tag="IVD64_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP6149397.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IVD64_002159"
     CDS             9425..11104
                     /locus_tag="IVD64_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6149398.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IVD64_002160"
     CDS             complement(11358..11558)
                     /locus_tag="IVD64_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6149399.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IVD64_002161"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IVD64_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3489 a         2584 c         2082 g         3845 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
//
LOCUS       DACBZN010000088        11440 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.088, whole genome shotgun sequence.
ACCESSION   DACBZN010000088 DACBZN010000000
VERSION     DACBZN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.088"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            184..1182
                     /locus_tag="IVD64_002162"
     CDS             184..1182
                     /locus_tag="IVD64_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6149400.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1294..1803
                     /locus_tag="IVD64_002163"
     CDS             1294..1803
                     /locus_tag="IVD64_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP6149401.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1833..2492)
                     /locus_tag="IVD64_002164"
     CDS             complement(1833..2492)
                     /locus_tag="IVD64_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP6149402.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2494..3258)
                     /locus_tag="IVD64_002165"
     CDS             complement(2494..3258)
                     /locus_tag="IVD64_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP6149403.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3266..3943)
                     /locus_tag="IVD64_002166"
     CDS             complement(3266..3943)
                     /locus_tag="IVD64_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP6149404.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3980..5365)
                     /locus_tag="IVD64_002167"
     CDS             complement(3980..5365)
                     /locus_tag="IVD64_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP6149405.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="IVD64_002168"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVD64_002168"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="IVD64_002169"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="IVD64_002169"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP6149406.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="IVD64_002170"
     CDS             6304..7161
                     /locus_tag="IVD64_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP6149407.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="IVD64_002171"
     CDS             complement(7242..7577)
                     /locus_tag="IVD64_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149408.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="IVD64_002172"
     CDS             complement(7644..9014)
                     /locus_tag="IVD64_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP6149409.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="IVD64_002173"
     CDS             complement(9092..9628)
                     /locus_tag="IVD64_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6149410.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="IVD64_002174"
     CDS             9937..11418
                     /locus_tag="IVD64_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6149411.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3515 a         2301 c         1854 g         3770 t
ORIGIN      
        1 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
       61 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
      121 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
      181 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
      241 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
      301 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
      361 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
      421 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
      481 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
      541 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
      601 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
      661 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
      721 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
      781 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
      841 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
      901 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
      961 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     1021 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     1081 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     1141 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     1201 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     1261 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     1321 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     1381 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     1441 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     1501 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     1561 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     1621 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     1681 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     1741 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     1801 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     1861 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     1921 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     1981 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     2041 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     2101 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     2161 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     2221 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     2281 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     2341 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     2401 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     2461 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     2521 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     2581 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     2641 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     2701 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     2761 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     2821 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     2881 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     2941 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     3001 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     3061 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     3121 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     3181 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     3241 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     3301 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     3361 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     3421 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcgctatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct
//
LOCUS       DACBZN010000089        11329 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.089, whole genome shotgun sequence.
ACCESSION   DACBZN010000089 DACBZN010000000
VERSION     DACBZN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.089"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(51..560)
                     /locus_tag="IVD64_002175"
     CDS             complement(51..560)
                     /locus_tag="IVD64_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149412.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDVSPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(550..1275)
                     /locus_tag="IVD64_002176"
     CDS             complement(550..1275)
                     /locus_tag="IVD64_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149413.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(1498..3618)
                     /locus_tag="IVD64_002177"
     CDS             complement(1498..3618)
                     /locus_tag="IVD64_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6149414.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3848..5026
                     /locus_tag="IVD64_002178"
     CDS             3848..5026
                     /locus_tag="IVD64_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP6149415.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5042..5800
                     /gene="aroD"
                     /locus_tag="IVD64_002179"
     CDS             5042..5800
                     /gene="aroD"
                     /locus_tag="IVD64_002179"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP6149416.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5823..6308
                     /gene="ybaK"
                     /locus_tag="IVD64_002180"
     CDS             5823..6308
                     /gene="ybaK"
                     /locus_tag="IVD64_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP6149417.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6379..7632)
                     /locus_tag="IVD64_002181"
     CDS             complement(6379..7632)
                     /locus_tag="IVD64_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6149418.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7749..9098
                     /gene="gdhA"
                     /locus_tag="IVD64_002182"
     CDS             7749..9098
                     /gene="gdhA"
                     /locus_tag="IVD64_002182"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP6149419.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9210..10556
                     /locus_tag="IVD64_002183"
     CDS             9210..10556
                     /locus_tag="IVD64_002183"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP6149420.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10864..11250)
                     /locus_tag="IVD64_002184"
     CDS             complement(10864..11250)
                     /locus_tag="IVD64_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6149421.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3683 a         2032 c         2299 g         3315 t
ORIGIN      
        1 atcatgaatc gatcttctat accgcttgtt tttactataa aatgattgat ttagcccgcg
       61 ggctctaaac gatcaatata aggactttga gcaatagtag catcatgagt aaagagtaag
      121 ataccttgat tttcttcatg agctaaataa gaaagcaagt ggagtagctc ttggatctcc
      181 aatacactaa gttcatcaaa tgtatcttca ataacaatat tgtttcgctt ggaaagcaag
      241 gcatgaagta attgcagctt tactttttca aaagacgtca actcagctgc cgcacaatta
      301 agcaaggaag gagagacatc aagtttttct tgataggcca aacgatcttg tttgatttct
      361 ttttccggta catccaacag gacatggtct ttaagattca gataaggaaa catcggccaa
      421 tttttttgga tgatcgcaaa attatccagt tttgcaacaa gaacctcttt tttcaagtgc
      481 tccaatattt tatgctgttt ttcgagagga cacaaaataa cggtcaagtg ttcagcatga
      541 atcaaatatt caggtttcat tacgtccagg cctcctactt tgcaaatgat ggataaaaaa
      601 agtgaaaggg aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc
      661 aaatccctta agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt
      721 ctgttgatcc atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg
      781 cagttcagta gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga
      841 ctggaagctt acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag
      901 ctgccagaaa ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat
      961 caattcctgc ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa
     1021 tagaaaaaat aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg
     1081 aagaagctct gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc
     1141 taaagccgat tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct
     1201 tgaaaataaa caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata
     1261 gcggattatt ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag
     1321 tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag
     1381 aggttgtgaa gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta
     1441 ttcgaaaaga ggagcagcga caataatttt gtcgctgctc cttttaaatt tattttatca
     1501 ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt tataaagatc
     1561 aacagttgat tgtccgttgt gttcactaaa gacactagtt gacttgtcac caagagattt
     1621 ttcagcttct tttaatttct ctaagccatt cttgtagtca taatcactgg tatcaattgg
     1681 gtctaatcca ctattcgtat agaaccgaag aagatcacca ttattgattt ggtcagaaac
     1741 agctaactgt ttatcaactt tttctttcag tgaatcaatt tcattctgta cttcctcagt
     1801 aggttcagta atagcttcac cagtttcgtt actatagatc gtaccaccgt agtaagtata
     1861 ttttggtgta acaaaatcac catcacggaa agaaacaagc tgttcatgat ctttcgagaa
     1921 tagatcttgt cctaactgaa tataattttt tgtatctaca cctagtaagt gaagtaatgt
     1981 aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg
     2041 gatatggatc atataaggga cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga
     2101 acttgttttt ccaactaatt cagctagatt tttatttctt gaattcgaga caccgtaatg
     2161 gtcaccgtaa aggacgatga ctgaattatc atacaagccg ctttctttca aataattgaa
     2221 gaattcttgt acggcggtat ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc
     2281 atcatttgta ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg aataaggata
     2341 atggttagat acagcaatga attttgaata aaatggttgt tgcagacgtt ccaagtattg
     2401 tacggattgt tggaaaaatg gtttatcatg caacccgtat tggaacgaat tgtcatcatt
     2461 aacatcataa taagaagaat caaagaaata atcatatcct aaattcttgt atgtctcgtt
     2521 tcgattccag aatgtaccag cgttcccatg gaataccgca ctaatgtaac cttgtgtttg
     2581 acttaaaata tcaggtgcag cttggaaagt attttttccg cctaattgtg tgaacaagga
     2641 gccttgattc aagccaaaca atgaattttc catcagcgtt tccgcatcgc ttgtttttcc
     2701 tgcttttact tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact
     2761 attgatgaaa ggtgtaactt catgctggag accattctca tctgtcaatt tataatcgat
     2821 caggaattgc tggaagcttt ctaaatggat ataaatcaca ttttttcctc tagcgagccc
     2881 gaacaaatcg tcatttggtg ctgcataatg ttcttttaca taattttcta cttctttcat
     2941 atcattggga cttgcttctg cccgcacttg gtttgtctgg taagtttgaa caccgtcata
     3001 aaccatgaaa gcattcaagc caagatattt gaccaaataa tcacgagaga atgttcgagt
     3061 cagcaattct ggacgatcag cttccgccat aaaaagattt cctgagaaaa tcataactgc
     3121 taatgtagag atcgcaaaag ccattcttgc tcggatcggg cgagggtcca acttgatttt
     3181 ttttgcaatc agcagaacag tcaagataat aaaatcgatc caatagatca catcatatgg
     3241 gcgaaacagt cgaatggcac tttctcccag accactcgct actttcccag cacctaaaat
     3301 cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa gtgacattcg aaaaaacaag
     3361 caatgtcaaa agaaaataaa tgaccatcat cgtgatataa gcaactctag ggcgtctgat
     3421 ataaagagca attccaagta ataacaatgt agttgcaata gggttgataa acaagataaa
     3481 gtattgaatg ccactttcga ttccgagatg gaaatcaact aaataggcaa aaatcgtttt
     3541 caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg
     3601 tgtttttata catttcaaga taatcttcct ttctgcactt cttaatcaca gaagtgtcct
     3661 gaaaccatgt tacacagctc acttattctc atttatttta ctagtatgac aatatagtgt
     3721 caattgaaac cagaagttta tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa
     3781 tgagcagtaa gacaagtcct atgttttttg ttatacttgg gaagagaaag ctaaagcagg
     3841 tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc
     3901 actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt
     3961 agttgattcg caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga ataagggaat
     4021 cggttgggta ttgagccagc agaacatccc atttgatcaa tctttttttg aagaattttt
     4081 tgtgaagtca aaagaaaaaa gaactttctt tttttcagat gattctacta ccgcttttcg
     4141 tatattcaat ggagaaggag acgggatcgg cggtctgatt gttgattatt atgcaggtta
     4201 tgctgttttt tcttggtata acgaaacgtt atatgcacat cgtgaattat tgattcaagc
     4261 ttttcgtgaa atctacccgg aaatcatagg aggctatgag aaaattcgat ttgaatcagt
     4321 aaatctgcct gagagccagt tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa
     4381 agaaaacgga gtaaaatttg ccgtttattt aaatgaagga ttaatgactg ggatcttttt
     4441 agaccaaaaa gaagtacggg gatcattagc agaaggtttc gcttctggga aaacagtttt
     4501 gaacatgttt agttataccg gcgccttttc tgttgctgcc gcaatgggag gggcaatcga
     4561 aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa acacaggaac agtttgaagt
     4621 gaatggtttg gatgtcgatc agcaaaaaat cattgttatg gacgtattcg agtatttcaa
     4681 atacgccaaa agaaaacaat ttttatatga catgattgta ttggatccac caagttttgc
     4741 cagaaataag aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg tcaaggatag
     4801 tctggatatt ttagcagaag acgggatact tattgcgtct acaaatgctg cgaatgtctc
     4861 gatagaacaa ttccaaacaa tgattgaaga ggaattccat gccaaaaatg tttcttttga
     4921 acaacttgct ttatatcgtc tgccaaaaga tttccagacg acggatacct ttttagaagg
     4981 caattatctg aaagtgtttg tttatcaaat taaaaataac cattagaagg agcgatgcag
     5041 aatggttcgg gtaaagggat tgaatatagg aagcggtaga ccaaagattt gtgcgcctct
     5101 tatcggggcc gtccgagagg aagtattacg tgaagcagtt cttgcacgtg aagcaggggc
     5161 cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc
     5221 agaaacattg gttcttatcc atgagatgct tgatggactg ccgttattat ttacgtttcg
     5281 taccttagct gaaggcggag aacaagacat atcgatccgt cagtatcgcg acctttatga
     5341 acatgtgata cacacaggat tagtagatat ggtcgatatc gagttgttca aagtagaaag
     5401 tctaggaaaa agactgatag aggaaatcaa gtctttaaac attcctttaa ttatcagtag
     5461 tcatgatttt aaagaaacac cagcagaccc tgtcttgctt taccgattga atgttatgga
     5521 acattttgga gccgatatcg gaaaactggc agtaactccc aataatgaac gagatgtttt
     5581 gcggttgatg gagttgacta gacgggcaaa tgcttttgtc tccatgccta ttattacgat
     5641 gtccatggga aatcttggta aaatttctcg tctggttggc ggaacgacgg gttccgttat
     5701 gacatttggt tccttaagca gcgattctac ttctgctcca ggacaattac cggtcaaagc
     5761 attgaagcaa attattcaat tattaaatga accgttgtag aacaagtttg aggtgatatt
     5821 ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga
     5881 ttccctatac agagcacgaa tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg
     5941 ctgaacagct tccagaaagc caatctcgta tatttaaaac actggttgct gtgggcaatg
     6001 tgacggggcc tcttgttgct gtgataccgg gagaagccga attgaatcta aaaaaattag
     6061 caaaagtttc agggaataaa aaagtagaaa tgctccattt aaaagatttg gaagcaacga
     6121 ccggatacat acgtggtggc tgctcgccta taggtatgaa aaaactattt cctacttatt
     6181 tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga agagggctac
     6241 agatggagtt agcacctcaa gatatctgtg cattaacgag cggacaattt gcagatataa
     6301 aacaataaat catacgtata aaagaaagaa ggaggccggg atgttgtttg tccagtctcc
     6361 ttctttcttt tttatacatt atgctctttg cccggcattc attcctgcta catgccctgt
     6421 agcgaaagcc gctgtgatat taaagccgcc agtataacca ttgacatcca gtaattctcc
     6481 cgcaaaatac agaccatcaa tgattttact ctccaatgtt ttaggataaa tttcctttag
     6541 agaaacccct ccgccagtta caaatgattt ctcaatagga aaagttttgt tgattgatag
     6601 cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc
     6661 tgtcagatga tccatttcaa gccgctcaag aaagaaaact aacaatcgct caggcaatat
     6721 accatgccaa gcattgacca agttcttttt ggttgcttta cttttttctg tcaatatatg
     6781 aataagctct tgtttggttt gtgtagggaa acaatccaga gaaagtgtca ctggttctcc
     6841 cgttttctct aattctttat tgacaaaact tgaacaacgc agtgcagccg gaccagaaat
     6901 accgaaatgg gtgaaaagta aatccatcgt atgttcagtc aatacacgac cttttttatt
     6961 taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga agtgttttct cctgaatata
     7021 cggttcatcc gatatcaagg gggactctgt cgcataaaga ggcgtcaccg tgtgacccac
     7081 tcgcttcacg atcttgtacc catctccagt agaaccagta gatggataag tccgtccacc
     7141 agttgttaag ataatggcag gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat
     7201 tttatgttct ttgtggatca atttctctac tcgagtagag aatagaagcg tcacatctag
     7261 ttctttcaga cgattgagca atgcttcgat aatcgttttt gatttatttg ttacaggaaa
     7321 catccgtcca tgatcctctt cttttaaatg tacgccttgt gattcaaaga agttcatgat
     7381 atcgaaatta ttccattgcg caaacgtact atataaaaac tttccatttc caggaatatg
     7441 tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg caccgaccac cacctgtcat
     7501 cagtagtttt tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc
     7561 agctgcactg atcgctgcca tcatcccgct tgttccagcg ccgactacga tcacatcata
     7621 cagtttttcc attccctcac ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa
     7681 aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc
     7741 gtggaacgat gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa
     7801 aggatcaagg gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt
     7861 cttttttgga cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac
     7921 cagaaagagt tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa
     7981 atagagggta ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct
     8041 tccatcctag tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga
     8101 atagtttaac cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag
     8161 gaaaatctga taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac
     8221 atatcggccc atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg
     8281 gctatttgtt cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta
     8341 aacctttgga tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt
     8401 attatgtcaa acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg
     8461 tttccggaag cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga
     8521 aagttgttac atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga
     8581 aactattgaa agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac
     8641 gtccttctgc tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata
     8701 tcgctttgcc atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag
     8761 aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt
     8821 ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag
     8881 tagcagtatc tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc
     8941 aagtggatga gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag
     9001 cagccgagta cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa
     9061 aagttgccaa agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat
     9121 attgtcagca ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct
     9181 gtagagtaaa cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag
     9241 tagcaccgtt tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc
     9301 acaacgaatt acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac
     9361 caacgaacta tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat
     9421 ctgattctga agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag
     9481 cgatcgactt cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac
     9541 cacaaatctt ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag
     9601 taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac
     9661 cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag
     9721 caaacaaacg tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag
     9781 atgcagaagg ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat
     9841 taactgctgt tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc
     9901 aaggtgccgc tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt
     9961 accaatacgc agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga
    10021 tcaactatga accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat
    10081 ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc
    10141 actctttagg acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg
    10201 ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag
    10261 gctacttgca aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc
    10321 ttgctcatac agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt
    10381 atacattggg ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga
    10441 atggaatcaa cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac
    10501 ttctaggcaa accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata
    10561 agggtttttc gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa
    10621 ctggtaccca tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga
    10681 caagacgttt gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag
    10741 tttgttttag gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt
    10801 tttctagaat cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt
    10861 agcttaagat aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac
    10921 ttttttctct atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta
    10981 agcagaacca ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc
    11041 accgttttca gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt
    11101 atctgctttg atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt
    11161 acctacataa gtagactgat agtagtccca acctttccat cctataaaag caatcaatat
    11221 gataacgatc aaccctaaaa tttttttcat gaaagatctc cttttttatt tttattatac
    11281 ccgtggtgct agttaatttt aatatcctaa agaatatctc aaagttcct
//
LOCUS       DACBZN010000090        11142 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.090, whole genome shotgun sequence.
ACCESSION   DACBZN010000090 DACBZN010000000
VERSION     DACBZN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.090"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            5..1189
                     /gene="iadA"
                     /locus_tag="IVD64_002185"
     CDS             5..1189
                     /gene="iadA"
                     /locus_tag="IVD64_002185"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP6149422.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            1300..1503
                     /locus_tag="IVD64_002186"
     CDS             1300..1503
                     /locus_tag="IVD64_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6149423.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(1604..2266)
                     /locus_tag="IVD64_002187"
     CDS             complement(1604..2266)
                     /locus_tag="IVD64_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149424.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(2279..3781)
                     /locus_tag="IVD64_002188"
     CDS             complement(2279..3781)
                     /locus_tag="IVD64_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6149425.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            4329..6008
                     /locus_tag="IVD64_002189"
     CDS             4329..6008
                     /locus_tag="IVD64_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6149426.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            6135..6614
                     /locus_tag="IVD64_002190"
     CDS             6135..6614
                     /locus_tag="IVD64_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP6149427.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            6833..7513
                     /locus_tag="IVD64_002191"
     CDS             6833..7513
                     /locus_tag="IVD64_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP6149428.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            7521..8852
                     /locus_tag="IVD64_002192"
     CDS             7521..8852
                     /locus_tag="IVD64_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6149429.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            8867..9829
                     /locus_tag="IVD64_002193"
     CDS             8867..9829
                     /locus_tag="IVD64_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP6149430.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            9940..10278
                     /locus_tag="IVD64_002194"
     CDS             9940..10278
                     /locus_tag="IVD64_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP6149431.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            10357..11064
                     /locus_tag="IVD64_002195"
     CDS             10357..11064
                     /locus_tag="IVD64_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP6149432.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3594 a         1980 c         2434 g         3134 t
ORIGIN      
        1 atgtatgaaa atcattcgac aaattgaagt gtttgcacca gattacttag gaaaaatgga
       61 tgtactgatt gccggtggga agattttggc tgtagaagaa cagctagaag gcggctacga
      121 aggagtggaa gtagaagaac tttcaggtga gggaaaagta ctaaccccgg gtttcattga
      181 ctgtcatttc catattttag gtggaggagg agaaggcggt tatcaaaatc gaacgccaga
      241 agtcacattg agccaattga ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac
      301 agacggagaa ggtagagata tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga
      361 aggaatctcc acttatgtat acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga
      421 ttctatcatc aaagactttt tgacaattga taaaattatc ggaatcggag aaatcgcagt
      481 atccgatcac cgttcttctc agccaagttt tgaagagttc gctcgtgcag tagcagatgc
      541 tcgtgtagga ggcatgttat ccggaaaagc tgggatcatc aatgtccact taggcggagg
      601 aaaaagaaag ctagagctat tgacacgaat cgtagaagaa acagaaattc cgatcacaca
      661 atttttgcct actcatgcaa atcgcacacc agaattattt gaagcttgcg tggcatttgc
      721 caaacgtggt ggcacgattg acttcactgc tagcgaagat cctgattttt gggagaagac
      781 agatggtgaa gttcgcttca gcaaagcact gaaacgattg atcgaggaag atgtttcttt
      841 agataatttc acgatgacct cagatggaca aggaagcttg ccttattttg atgaaaacaa
      901 tcatttccta gggttaggag taggaagtgc caaggcattg ctagtaggga tcaaagaagc
      961 tgtgcaaaaa gaaagtatcc ctttggaaat cgccttacga gctataacca gtaatccagc
     1021 acgtatattg aaattggata aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg
     1081 tatcttggat aaagaaacgc ttgatattga tacagttatc gctaaaggag aaataatggt
     1141 ccaagaaaaa gaagtcaaag tatgggggac tttcgaaaaa agtttttaat caacagctct
     1201 aactatcttt tttagaaaac gagcgttctg tttgtctgta tctgttgttg atgttaccct
     1261 gaaggtagat caataaacaa actaagagga ggaagcacaa tggcagattt aaaaggacgt
     1321 tctaaagata tgaaagataa agtagtcggt gaaggaaaag aagcatacgg caaagcaaca
     1381 gacgacaaga gcaaagaaac agaagggaaa gcacaatcac taggtgctga catcaaagaa
     1441 aaagctcgaa atttaggcga cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa
     1501 taaaaaccac ttattactaa ttgaatcaag caaataaaaa atccgaacca tatctgcgag
     1561 acagatatgg ttcggatttt ttatttgatc attatcctgt tttttaaact tcgatttttt
     1621 gaaatttttt attttttagt tcaaagactt catcacagct ttttgctact tcaatctcat
     1681 gagtcacgat gataatacag cgattttgtt catgggcgat ttcttggaaa agtttgacga
     1741 tatcttgtga agttgtttca tctagatttc cagtaggctc atccgctacg attagttgat
     1801 gttcacagca gatagcacga acgatagcga ctcgctgctg ctgacctccc gaaagtttgg
     1861 tcacctgttt ttttgctaat atttcatcaa ttcctacacg agccagtcga tctaaagcaa
     1921 cttgtcgata attctcttgc ttggtcttag tgatttccat tgctgctatg acgttgtcta
     1981 gagccgataa ataaggaagc aagttgtaag cttggaaaat aatcgagaca tcatttttac
     2041 gataacttct taacccgatt ttgttcagtg aacgatcttg atagtatacc tttccttctt
     2101 ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc
     2161 ctaaaatagc gtacattttt ccagcttcga aattcagttc tacatcttta taaagcgcat
     2221 cttgcggatc ttgataccaa aatcccaatt gttctgtccg taacatcaaa ttcctccttt
     2281 agtttaatag gatttttctt gggtttaatc ttaatatccc tatagaagct aaaaagacag
     2341 aaaacagact gattccaaat gctactccgg ctaatagtcc caattgtgca ggtttgacat
     2401 tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg attattcgtg gttccttgtt
     2461 gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg catttgtcca ccgccaggag
     2521 cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag tagctgctgt cccacggcat
     2581 tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc aagaatcata caaatagcca
     2641 cttcggtaaa caattggaga atgattttac ttctggattc tcctaaagat agcaagacgc
     2701 ctaactcatg ccgacgctcc cgaatggtaa tcataatgat cagagtaagt atgataatac
     2761 cggctacagc cactagtaaa acaacatttt tagaaaaact tgccacgttg tttaaaggtt
     2821 ccaacattga ctgatacatg gaatcatttg attgtaagct aaatgtatct gtgtcgatca
     2881 gcttctcagc ttctttgaga aactcatcca ttttatctgg atcagataga gtgtaaatag
     2941 cagaatctat cgtgttatcg tcagatgtcc catttagttc atttgcaaaa gtgtaagaag
     3001 tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat acccatactg ccgcttgttt
     3061 cgctggattc ataaattcct ttgatcgtca tttcataagt ggtatcttca tcttcagtac
     3121 tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa
     3181 tgactacatt gtttgtgcct ttgtcgctgt catctattcc ttcaccatcc gtgatttttg
     3241 ctgttccatc agaaaaatta ctattttgtg cagttgctga cacaccactg atttgaaagt
     3301 ctgcttgagt catttgcgga ttcccgccca ttcctccagc aggttcaccg cctgcatcag
     3361 tagtttctga actgtcttca cttgaatcag aactagtgat ggcagttatt ccatcttttg
     3421 ccatagctga ggcggaactt tcaaaggaat aactctttac attatctaat tcagctattt
     3481 tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact gcctggatct ggacgttctt
     3541 cagtggtaga accgcttgaa gaagtttgtt cctgtttttt aaatgaagct tctcgattgg
     3601 cacttaaagt cactgttgct ccaacttctt tttgtgcttg attcgctgct tcttctgcag
     3661 cgcttcgaat cgtgaggcca gctagcacaa aaataagaat ggctgtgaac acggcaaaca
     3721 ataacaatga acgtccccat tttacttttg tacttttcca tgcacgtttc ataaaattca
     3781 tattttgcat ccctcctagt agatgcattt gataatagac aacctatctt aaagctagct
     3841 taaatcttat gctgtttgca caccatcaac atgaggttct gtatatataa aaagtcacta
     3901 tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa
     3961 atgaaagagg aatagcaaaa gaaatcctag atgctacaga gggattttta cgtgcatagt
     4021 tgttctaaca attgctgata ctagcgattt agtttatttg ttgtataata atggggaaca
     4081 gctgaatact tatataagta ttctggctat agcgtttcgc aaaagatcca ttttgttaat
     4141 gaagtatatc caaaataaaa tgcccatcca actgtcagaa cgcggatgtg ctggtaattt
     4201 tcctgctgat tctccataag ttgagaaata gtgattttgc atatgcttca ttggaatggc
     4261 tcatatattt agataaagaa gaaaatcgac atctttaaac ttggatgtca taagaagagg
     4321 tgaagtttgt gagtaccatt aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa
     4381 acatgtatgt cgcagaagtg ggcgaggata tttttatttt agattgtggg ctaaaatatc
     4441 ctgaaaatga gttgttgggg atcgacgtcg taatccctga ttttacgtat ttagaagaaa
     4501 acattgatcg tgtagctggt gtctttttaa cacatggaca tgcagatgcg atcggggcac
     4561 ttccttactt gttgtcaaaa atttctgtac cagtattcgg gacagagttg acgatcgagt
     4621 tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg atttaaggat ttccatgtgg
     4681 tagatgagca tacagaaata gactttggtg aaacagtagt gagctttttc cgtaccactc
     4741 atacgatacc agattcagtg gggatcaatc tgaaaacaaa agaaggtagt atcgtttata
     4801 cgggagattt taaatttgac caaacggcta tcccaatgta tcaaacagat tatgcacgat
     4861 tggcggaaat cggcaaagaa ggagtcttag ccttactaag tgaatcggca aatgcagaaa
     4921 atcctcagcc tgtagcttct gaagcccaaa tagcagatga agtatttgat acgatcaaat
     4981 attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag
     5041 tattggatgc agcagaaaaa gcacaacgaa aagttgtctt gactggtcaa gatttcgaac
     5101 gaattattcg cacagcgatg cgtttagaaa aacttcaatt accaagtgaa gatctgcttg
     5161 ttacgttgaa agacatgaaa aactatgatc cagaagaact gatcatttta gaaactggcc
     5221 gaatgggtga acccatcaag tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca
     5281 agattgaaga aggagatttg gtttatatca ctactacgcc aagcatcgcg atggaaacca
     5341 tcgtggcgaa aacggaagat atcatttatc gagctggtgg aacagtgaag ttgatctctg
     5401 agaatatgcg cgtttctggc catgccaatc ctaatgactt gcaattaatg atcaatttga
     5461 tgaaaccaac atattttgtg ccagttcaag gagagtatcg tgaattagct gctcatgcag
     5521 atttggctca tgcagtaggg atgccataca aaaatatcta tattactgga cgcggagacg
     5581 tgctggaata taagaacaag cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa
     5641 tgattgatgg attaggcata ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct
     5701 tgtcagaaga cgggattttc gtagcggttg tcactatcag tcggcgagaa aagaaaatca
     5761 tttcaaaacc tcaaattact tcacggggat tcgtttacgt aaaagctagc cgtgatctga
     5821 tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa acatttagaa agcaatgatt
     5881 ttgagtggag caaattaaag caagacatcc gagatcaatt aggccgttac ttatttgaac
     5941 aaacaaaacg ccgcccagtg atcttgccag tcattatgga agctactcaa cgtcgaaata
     6001 aaaaataaac aaaaagagta agatgaacct ctctatagag tgctcgtctt gctctttttt
     6061 tgcgaaaaac aaataatttt tgaatacagc atccctcgct ttataataaa cgatagaata
     6121 aagggagtga atcaatgatg agtcaaacaa aagtcgaacg ttatttagaa gaacaaaaga
     6181 tcagctatca accacttgat tttagtcatg tggtagaaga atctttcaca gaagaactac
     6241 aaaaaagagg gattgatcca gctttgatat gtaaaactct cgtagtgaaa ggaaacaaaa
     6301 ctggggtgat catcgcattg attcctttga acgatcactt agattacaaa aaaacgagac
     6361 aagtgacgaa agatcgtaaa gttggttttc ctggaatgga ttttgtatta atgcatacag
     6421 gatatcccca cggagcaaac acccctattg gaataaaact cgcacatcct gattattgtt
     6481 ttttagcaga cagttcttta aaagaaaaag aagaagtaat tgtgtcaagc ggtgaaatcg
     6541 ggagaagcct tcggataaaa gtagccgatt tagaaaagat cgtccatcct atttacgctg
     6601 atttaattag ttaagggacg agaagagaag ctaagcagag cagagacctc aaacttgaga
     6661 ttatatctgc ttggtacttg gtgcatcctg tattatctgt tacaattgct ttgtacatac
     6721 tgtactatca atccaatgaa aatatattta gcagctatat tttttcattg atgtgcaagt
     6781 gatgaaatcg tttttaataa aaaagataca gagaaaacgc gaggtgtaaa atatgaaaat
     6841 tacgtatcat ggacattcct gtttaacaat tgaaatggaa aatggccaac gcgtgatgat
     6901 tgacccgttt attacaggaa atccattgac agacttaact gcagaagaag taaagacaga
     6961 ttggttgctg attactcatg gtcatagtga ccacatagga gatatgattc ctattgcaaa
     7021 agaaaatcaa gccacaatta tcagtattgt tgagattgct gattatgcga acagcagagg
     7081 agtagattca ttcggtatga atatcggtgg aaaacatgcg tttccattcg gcgtggtaaa
     7141 attcgtacat gcacagcata gttcaagcta tgaagtagac ggtatcgttc agtatatggg
     7201 cgagcctagc ggaattatta ttcaagcaga aggaaaaacg atttatcatg caggggatac
     7261 tgcttatttc agtgatctag gattgcttgc agaagaattc gatattgatg tcgctttctt
     7321 gccgattggc gataactata cgatggggcc agaagatgca gcaaaggctg cgcaaacaat
     7381 tcgtgccaag aaagtagttc caatccatta caatactttc cctgtcattg aacaagatcc
     7441 cgaatctttt gtagagttac tggaagatag tgaaggaaaa ataatgagcg tctcggaaac
     7501 gctggaagtg taggcagaaa atggcgacaa aacatgattt gattttagaa tatattgaaa
     7561 gtttacctgt tggcaatcga atttctgtaa gaagcatcgc aaaagaattg aatgtcagtg
     7621 aaggaacagc gtaccgtgca atcaaagatg cagaaaatgt cggactagtt tcaacaattc
     7681 aacgagtagg gaccatccgt atcgaacgca aattaaaaaa acatattgaa cgtttgacat
     7741 ttggcgaggt cgtcaggatc attgaagggg acgttttagg cggggcggca ggattagaca
     7801 aggtcttgaa taaattcgtc atcggcgcga tgactaaaca tgccatggaa cgctatatta
     7861 cgccaggatc actaatgatc gtcggcaacc gggcggaagt ccaaaaatta gcattggaag
     7921 atggcgccgc agtattgatc acaggaggat tcaatacgac tgctgagatt gcaaagctag
     7981 ctgatgatct tgaaatgccg atcttacgca cctcttatga tacatttaca gtcgcaacca
     8041 tgatcaaccg agcgttaagc gaccaattga tcaaaaaaga tatcatgctt gtcagtgata
     8101 tctatatgcc agctgaaaag actcattatt tgcagcagac agatacgatt tctgattatc
     8161 agcgtttgtc agaagaaacg cagcattcgc gttttccagt cgtcaaccgc catcatcgtt
     8221 tgatggggat tgttacggca aaagatgttt tagggaaatc gccaaaccag atcatcgatc
     8281 gggtgatgac gaaagaacca atcagtgtaa agaaaacaat gagtattgcc tctgttagcc
     8341 atcaaatgat ctgggatggg ctagaagtca tgccggtcgt ttccgatgac ttgacattgg
     8401 aaggcttgat tacccggcaa gatgtaatga aagcgatgca gcttgtccag cgacaaccgc
     8461 agattgcaga tacgatttcg gatcagatat ctggaaatat ccatacgatt gatactgacc
     8521 gtgaaggaaa tcgtttggaa aaccctaagt tcaaattcgt agtagcacca caaatggtca
     8581 atggcgttgg aaccatttct tttggcgtac ttagcgaaat catttctgat gtcgcacaaa
     8641 aaacaatggt gatgaaccaa aaacgaaata tcttgattga acaagtgaac ctacattatt
     8701 tgcggctgat ccaattggaa agcgaattag acattcgtcc acgtgtattg gaaatcggca
     8761 gacgttccgc taaactggat atcgaagtgt ttattgaaaa caccatcgtt tccaaagcaa
     8821 tcgttgtttg tcaagtaatg gaacgttctt agaaagcagg agtgaaatga cagtacagca
     8881 agaaatagta gatacaataa aaaaatacga taaaatcatt atccacaggc atttgcgccc
     8941 tgatcctgat gcattaggat cacaagtagg attagccgaa ctattacggg aaagtttccc
     9001 agaaaaagaa atacgtcaag taggtgaaac gattgaaggg cttcgctttt tagcagaaat
     9061 gcaagaagtt gaagatgtct tctaccaagg agcgctagtc atcgtgactg atacagctaa
     9121 ttcacccaga atcagtgatc aaagatatca gttggcagaa aaactgatca aaatcgacca
     9181 tcatcccaat gatgaccctt atggagatct tgtgtgggta aatacaaaag caagtagctg
     9241 tagtgagatg attgcagaat ttgctttgat gtttcctgaa tctttaaaga tgaacgttaa
     9301 ggctgcacgg ttactttatg caggaatcgt cggagataca gggcgctttt tgtatccctc
     9361 cacgacgtcc cgtacattag agattgcttc gatattgaga aattatccat ttgatgcaag
     9421 tgctttgaac agagagattg aacaaatgcc gatgaaagtc gctaaacttt ccggttatct
     9481 ttatcaaaat atccaggtgg atgaaaatgg tgcaggaaga ataatccttc cgcaagaaat
     9541 tcttgataaa tatggaatcg atgattcaga gacagcttcg atcgtttcac ttcctggagt
     9601 gattgacgaa gtgctggctt gggggatttt tgttcagcag ccttcagggt attaccgcgt
     9661 acgtttacgt tctaaggggc cgatcatcaa tgaattggct aaacgccatc atggaggagg
     9721 ccatccatta gcaagtggtg cgaatgcaaa agatttagca gaagtagagg aaatctataa
     9781 agagatccaa gatatatgcc aatcgtataa gagcagaaaa acagaatgat acttcgttcg
     9841 aaaacgtgcc ttttgtagta aaaatcacaa atgtatcttt tcaagatctt ctttttccgt
     9901 tacaatagtg ctcactaaag attttaaagg agaaagaaga tgacattacc aaattgccca
     9961 gaatgtggct cagagtacac atatgaagat cgaggcttgt tgatttgccc agaatgcggg
    10021 aacgaatgga acaaagaaga acaagaaact gtcatagaag acggattagt ggtattagat
    10081 gcaaatggaa accgtctgtt agagggcgat agcgtgacag tgattaaaga cttgaaagta
    10141 aaaggtgcgt caggtgccat taaacaagga acaaaagtca agaacatccg tttagtagaa
    10201 ggcgatcata atatcgattg caaggtagaa ggatttggac cgatgaaact gaaatcggaa
    10261 tttgtgaaaa agaattaaag aatattcaag gtcttttcac attcttttcc tactactaat
    10321 gataaaataa agtaaacgaa aaggagtgaa acgaaaatgt atggttatgg aatgtatggt
    10381 tttttagatc cgacatttct tttggtcatt cttggattag ttatttccgg aattgcgtca
    10441 gcgtacgtca atagtacttt ccgaaaatac gatcaagtga gaagcaaaaa caatgtgact
    10501 ggtacacaag cagcacaata tatattacaa agtcaacaga taaataatgt cggtgttcaa
    10561 caaattgccg gagatctcac agataactac aattcaggta ataagatgtt gagtttgtct
    10621 caagcaactg cacagtctac ttcagttgct gcgataggtg tagcagctca tgaatgcgga
    10681 catgcggtac aagatgcttc gggttatgtc ccattgaaga ttcgtgcagc acttgttcca
    10741 gcagctaact ttggttcgat gatttcgttc cccttgatta tggtaggtgt attgcttagc
    10801 tggaactcta cgttaatcaa tatcgggatt ttcgcttttt ctttagcatt acttttccaa
    10861 ttggtgacat taccagtcga atttaacgcg tctcggcgag caatccaaat tttgagtgaa
    10921 ggcggactac tgacggaaga agaagtaccg atggcaagac acgtattatt tgcagcagca
    10981 ctgacttatg ttgcagcagc gctttcgaca ttcctgcagc tgttgcgctt gatcttattg
    11041 ttcggcggca accgaagaga ttaaaataaa gaagttgtga aatcagcgct ttgatctgag
    11101 ctaataccga gagttagctg attgaatacc atttatttgg aa
//
LOCUS       DACBZN010000091        10980 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.091, whole genome shotgun sequence.
ACCESSION   DACBZN010000091 DACBZN010000000
VERSION     DACBZN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.091"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            191..709
                     /locus_tag="IVD64_002196"
     CDS             191..709
                     /locus_tag="IVD64_002196"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6149433.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            837..1520
                     /locus_tag="IVD64_002197"
     CDS             837..1520
                     /locus_tag="IVD64_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP6149434.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1829..1902
                     /locus_tag="IVD64_002198"
     tRNA            1829..1902
                     /locus_tag="IVD64_002198"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Arg,seq:tct)
     gene            2061..2420
                     /locus_tag="IVD64_002199"
     CDS             2061..2420
                     /locus_tag="IVD64_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP6149435.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2543..4174
                     /locus_tag="IVD64_002200"
     CDS             2543..4174
                     /locus_tag="IVD64_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP6149436.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4236..4877)
                     /locus_tag="IVD64_002201"
     CDS             complement(4236..4877)
                     /locus_tag="IVD64_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6149437.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVPPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5159..5467
                     /gene="rplU"
                     /locus_tag="IVD64_002202"
     CDS             5159..5467
                     /gene="rplU"
                     /locus_tag="IVD64_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP6149438.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5494..5838
                     /locus_tag="IVD64_002203"
     CDS             5494..5838
                     /locus_tag="IVD64_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP6149439.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5851..6144
                     /gene="rpmA"
                     /locus_tag="IVD64_002204"
     CDS             5851..6144
                     /gene="rpmA"
                     /locus_tag="IVD64_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP6149440.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6237..7385)
                     /locus_tag="IVD64_002205"
     CDS             complement(6237..7385)
                     /locus_tag="IVD64_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6149441.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7397..7708)
                     /locus_tag="IVD64_002206"
     CDS             complement(7397..7708)
                     /locus_tag="IVD64_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149442.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7761..8156)
                     /locus_tag="IVD64_002207"
     CDS             complement(7761..8156)
                     /locus_tag="IVD64_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6149443.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8192..8536)
                     /locus_tag="IVD64_002208"
     CDS             complement(8192..8536)
                     /locus_tag="IVD64_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6149444.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8837..9058
                     /locus_tag="IVD64_002209"
     CDS             8837..9058
                     /locus_tag="IVD64_002209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149445.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9185..9961
                     /locus_tag="IVD64_002210"
     CDS             9185..9961
                     /locus_tag="IVD64_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP6149446.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            9958..10152
                     /locus_tag="IVD64_002211"
     CDS             9958..10152
                     /locus_tag="IVD64_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6149447.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            10154..10438
                     /locus_tag="IVD64_002212"
     CDS             10154..10438
                     /locus_tag="IVD64_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149448.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10459..10647
                     /locus_tag="IVD64_002213"
     CDS             10459..10647
                     /locus_tag="IVD64_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149449.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
BASE COUNT         3528 a         1786 c         2106 g         3560 t
ORIGIN      
        1 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
       61 ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc
      121 tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat aaagaaagaa
      181 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
      241 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
      301 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      361 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      421 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      481 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      541 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      601 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      661 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      721 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      781 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      841 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      901 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      961 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
     1021 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
     1081 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
     1141 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
     1201 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1261 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1321 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1381 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1441 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1501 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1561 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1621 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1681 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1741 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1801 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1861 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1921 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1981 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     2041 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     2101 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     2161 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     2221 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2281 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2341 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2401 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
     2461 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
     2521 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
     2581 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
     2641 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
     2701 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
     2761 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
     2821 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
     2881 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
     2941 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
     3001 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
     3061 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
     3121 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
     3181 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
     3241 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
     3301 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
     3361 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
     3421 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
     3481 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
     3541 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
     3601 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
     3661 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
     3721 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
     3781 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
     3841 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
     3901 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
     3961 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
     4021 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
     4081 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
     4141 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
     4201 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
     4261 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
     4321 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
     4381 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
     4441 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggcggg
     4501 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
     4561 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
     4621 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
     4681 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
     4741 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
     4801 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
     4861 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
     4921 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
     4981 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
     5041 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
     5101 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
     5161 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
     5221 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
     5281 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
     5341 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
     5401 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
     5461 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
     5521 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
     5581 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
     5641 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
     5701 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
     5761 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
     5821 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
     5881 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
     5941 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
     6001 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
     6061 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
     6121 tatccagtag ctcaagaagc ttaataaata aactgctcta tctcttgtgt agcaagggat
     6181 ggagcttttt tgtaaattat gggatttttt ttgaccatac ttttgaccat actttcttag
     6241 aagttaatat aatttgttag tttttgtgtt gtttcttctt ttttgctgtt agtaatgtgt
     6301 gtatatgtgt ccatagtagt ttttatattt gcgtggccca ttctttcttg gacttctttg
     6361 tgatctgcgc ctgcctcata tagcattgat gcatgtgtat gtctaaaacc atgcagaccg
     6421 atataaggta agccggcacg ctcacgatat atattgtatc tttctgccac tgactgatta
     6481 attgtgaaat tattttcgtc attggtaaac aaataattag ctttagtaaa tcctaattga
     6541 aataagtact ttctttgttc gagcttccaa aattttagtt gctttatagt tttttcatct
     6601 aaataaatta ttcgatttga ttttttggtt ttaggagttt cagatatata atatttatct
     6661 gaacgagcta cagttttttt aatggccatt tcatttgttt taaaattaat attgtcccaa
     6721 gtgagtgcta atatttcgcc gattctacat ccgctgaatg ctaatagtct aaagagcgta
     6781 tagtctctgt ttttaaaata tggattctgt tcttgagata cttcatttag aaataattcc
     6841 aactgttctt ttgtatataa ttttaatttc ttttctgttt taatttgtgg ctttggaata
     6901 agtaaattta atgttggatt atcagatgtt aaaccaatat ttatagcata cttaaaaact
     6961 ttattcatat agtttatgaa aagagggtac tgctttgaag ttcctttttc actccaatca
     7021 tttactacct tttgacaata tgccgtatta attttagaaa gcttaatatt tccaaatttg
     7081 ggatatatat gtttattaaa gattatttta gtccttgaat atgaactttc ttttacagtt
     7141 tttttgtagc tttctagcca taattctgct gcttctttga aagtagtatt tgtacttgtg
     7201 ggcattcctg ttttttgtaa ttctagttct aacctagaaa gtgctgtttt tgcttctttt
     7261 tgtgttctaa atcctcgttt agtagtatat tttttctttc cagttagcgg atcaataccg
     7321 agataagttt taaagtacca tgctttttcg ccatttttct ttttatattg ttttatcatt
     7381 gccatcaata ttacccctat tcaaattcta attctgccat gctatcattg aaaaattcga
     7441 tcagtccttg ttcctctata aattcaagcc atatttcctt cccgttaatt aatggaactc
     7501 ctttttcttt acgaattgtt tgatacaagt tagtcacgac ttgtttccac atttctaata
     7561 cagctttatc tattttctta tcatcccagc gttttttttg gggtctattt ttgttttttt
     7621 caatttgata attagtagct ttgatgataa atgtttcgta tccggttgtg cttttttcta
     7681 accattcttt gaattctgta tatatcattt ttgttcacct ttctattttt gaacgtatgt
     7741 tcttttttgc ttaaaaaatt ttagagagct gcataacaat agatgcgagc ttttaataat
     7801 tctttcacaa gtggttcgaa gcgaacgctt aaattgttat cttctataaa tttcatgtaa
     7861 ttgatattgt gtggttctaa ttcagacttt gcaatatatc tatctaataa tttttcaatc
     7921 atgaagcgat cagcctcata ttccattttt gaatggagag agaatgctaa gttatataaa
     7981 aaataattat catgatgttt agaagcgtgt cctaattcat gtaataaggt tctttgtctt
     8041 ctatacttag ataaattagc ttttattact atggtattca tacaagcaac ataatggcca
     8101 tcggcatcaa ggttttcacg ctcttctacc ttaacgccga gttcattaat tatcatttca
     8161 atttgactgt ccaaatgact cacctactta tttagttatc ttttttctct aaatatgctt
     8221 caatcatagc tgatagtatt tccctatcat tatcggtgag cggttttccg tcgctactca
     8281 taacagatgc taaagcttct tccacggtta gttttctttc ctctggtgca actcccgcgt
     8341 taggatcatc tgtgcgtcct agcaaataat ctgttgatac atggaagtag tcagcaactt
     8401 tttgcaaagt ttctatttta ggactttgtg tcttccattt ataaatagag ttttttccaa
     8461 atcctattct attttctaat tcagaaatag aaatacctct ttttttacag agctctttaa
     8521 ccctttcgaa aagcatgtca aatcaacctt cctagagcgt acgataataa tttatacaaa
     8581 aagatagaaa ggtagttgac aaatctatcc aattgtctta tatttaactc gtaagctaat
     8641 ttaataagct aacaagcaac aagaaataca cctaataaaa taaacgaatt cgaggtcggc
     8701 aaactaagaa tgttaatttt tagggttttt ctaggcttat ttaactgtat ataaatataa
     8761 gacagttgga tagaaatgtc aataaaaatt agctaatttt attagcttac atatttaaag
     8821 aaaaggagga aacatcatga aacaaaataa aaaaactcct tccgcaaaag gagctttaat
     8881 aactttaaat tttaaagacc aaaagtttag tgctgttgtt gatgaaattc atccacaaga
     8941 gcatcaaaaa aattcttttg caactgacga gaaaaagact cagaaacgct ctgagaaagt
     9001 tcttcgaata attcaatttc tgttggaaaa gattcgtcat tttggaagtc tattttgaat
     9061 tctttattaa cagtcttgaa tacggtttgt ttgaataagg cgtcgacatc catattaaat
     9121 tcttccatac tattttcacc tcgctttcta ttcaaaatta tatcaaagaa aggaatgagc
     9181 aaaaatgaac acaccgcaaa tttttaactt tgaacaacat gaagtaagaa cagtaacaat
     9241 acatgatgaa ccattttttg ttggaaaaga tgtggctaaa gttctagggt accaaaatgg
     9301 tagtagagat attaacagac acgtcgatgt agaagatcgg caaaactacc aaaacggtac
     9361 ttttgaatcg cctagagggt taactattat taacgaatca ggtttgtact ccttaatttt
     9421 aggaagcaaa caaccaaatg ctaagaaatt taaacgttgg gtgactagcg aagtgttgcc
     9481 agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa
     9541 tccagataca atcatccaat tagcaactaa gttgaaagaa gaacgtactg gaagactaat
     9601 cgcagaacaa aaaattgcag aatacgaacc caaaatttcg tatttggata gtatcttatc
     9661 ttctacagat tcagtaacta ttagtcaaat cgcggctgat tatgggatgt ctccacaaca
     9721 gatgaacaaa ctacttcata agctaggtat tcagaaaaaa gtaggcaacc aatggttatt
     9781 atgcaaaaaa cacatgagac agggttacac aaaatctcat acaactgaga ttccgaaatc
     9841 tgatggcgga acaaaagttg tgatgaatac aaaatggact caaaaagggc gtctgttcat
     9901 ttatgagtcg ctgaaaaaag aaggatatat tccagaaatc gatttattag aggaggaatg
     9961 acgatgtcac aagaatttat tttgaaagtc agaattcaat tagcaaaata cggaaagtct
    10021 cagaactggt tagctgatac tatcggaatt tctagaccat atatgtcgga cattatgaat
    10081 gggcgtagaa aaccagataa acaaatcaag ccaattgagg cagcactagc agagttagag
    10141 aaggagaaat aacatgcaaa taacaattcc agataattta gtagtttccg aattaactac
    10201 acagattacg aatgctgttc ttaattcatt ggacgaacga ttacacctta tgaacaaatc
    10261 agtagagctt cctccatatc caaacaaatc agaggtaaaa aaagttttag gcattggtga
    10321 tgacaaatta acacattgga taagcctagg cttaaaaaca cagcagtgga gcaagttaga
    10381 catcagaatt gaacgatcgg aactccaaag atttttgaaa gaaaactttg agttctaaag
    10441 gcaaaggaga atgattttat gtcctacaca ttgcaacaag aacatcaaat tctaggtctg
    10501 attaaacagc gtagaaaaca attacaagat gatcgtgcag cgcttagaaa atccgatgag
    10561 ctatcagata gacaagctga actaattgct tctgaacttg aggatttgag aatgctagaa
    10621 ataaaaaata gggagattag attatgaaga agacagacac actttttata ggattcattt
    10681 tggggttatt agtgattgta gcgcaccaaa gtattatcgg aggaagcttg ttcgcagcat
    10741 tgatggtttt aatcaatctg cttgactcaa aagaaaggag caactatggc acgagaagaa
    10801 gcgctaaaaa tcggtaaagt gattgctgat aattggtggg caaatagccg ttctattatt
    10861 ttaagcaagc aacatatcga aaagaaaaaa gcatggcaac aaataaaaaa gtgactccgc
    10921 cgaccaaagc aatgagtcac tcaaaaaatc ataactaagg agattttaac atatggaaaa
//
LOCUS       DACBZN010000092        10936 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.092, whole genome shotgun sequence.
ACCESSION   DACBZN010000092 DACBZN010000000
VERSION     DACBZN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.092"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..154
                     /locus_tag="IVD64_002214"
     CDS             <1..154
                     /locus_tag="IVD64_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6149450.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /locus_tag="IVD64_002215"
                     /pseudo
     CDS             complement(235..1218)
                     /locus_tag="IVD64_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="IVD64_002216"
     CDS             complement(1613..2455)
                     /locus_tag="IVD64_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6149451.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="IVD64_002217"
     CDS             2780..3196
                     /locus_tag="IVD64_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6149452.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="IVD64_002218"
     CDS             3193..3663
                     /locus_tag="IVD64_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6149453.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="IVD64_002219"
     CDS             3677..4450
                     /locus_tag="IVD64_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6149454.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="IVD64_002220"
     CDS             4464..5276
                     /locus_tag="IVD64_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6149455.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="IVD64_002221"
     CDS             5330..6295
                     /locus_tag="IVD64_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6149456.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="IVD64_002222"
     CDS             6432..9155
                     /locus_tag="IVD64_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6149457.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="IVD64_002223"
     CDS             9168..9800
                     /locus_tag="IVD64_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6149458.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10005..10232
                     /locus_tag="IVD64_002224"
     CDS             10005..10232
                     /locus_tag="IVD64_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP6149459.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10301..10567
                     /locus_tag="IVD64_002225"
     CDS             10301..10567
                     /locus_tag="IVD64_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6149460.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="IVD64_002226"
     CDS             10557..10826
                     /locus_tag="IVD64_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6149461.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3626 a         1742 c         2137 g         3431 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tcaaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc tttttt
//
LOCUS       DACBZN010000093        10761 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.093, whole genome shotgun sequence.
ACCESSION   DACBZN010000093 DACBZN010000000
VERSION     DACBZN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.093"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            6..1073
                     /locus_tag="IVD64_002227"
     CDS             6..1073
                     /locus_tag="IVD64_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP6149462.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            1070..1897
                     /locus_tag="IVD64_002228"
     CDS             1070..1897
                     /locus_tag="IVD64_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6149463.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            1942..2967
                     /locus_tag="IVD64_002229"
     CDS             1942..2967
                     /locus_tag="IVD64_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6149464.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            2964..5147
                     /locus_tag="IVD64_002230"
     CDS             2964..5147
                     /locus_tag="IVD64_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6149465.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            5147..6277
                     /locus_tag="IVD64_002231"
     CDS             5147..6277
                     /locus_tag="IVD64_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6149466.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            6274..6951
                     /gene="pgmB"
                     /locus_tag="IVD64_002232"
     CDS             6274..6951
                     /gene="pgmB"
                     /locus_tag="IVD64_002232"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6149467.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            7035..7562
                     /gene="msrA"
                     /locus_tag="IVD64_002233"
     CDS             7035..7562
                     /gene="msrA"
                     /locus_tag="IVD64_002233"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6149468.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7763..8449)
                     /locus_tag="IVD64_002234"
     CDS             complement(7763..8449)
                     /locus_tag="IVD64_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP6149469.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            8529..10067
                     /locus_tag="IVD64_002235"
     CDS             8529..10067
                     /locus_tag="IVD64_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP6149470.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            10187..10654
                     /locus_tag="IVD64_002236"
     CDS             10187..10654
                     /locus_tag="IVD64_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP6149471.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
BASE COUNT         3532 a         2023 c         2414 g         2792 t
ORIGIN      
        1 aaaagatgaa aaaattagtt gcattagaac caagaatagc cggattcaaa gattacgaag
       61 accgtgagat cgaaaagaac gaagtacgta tccgagtaat tttcgctact ccaaaaccag
      121 gaacagaaat tgtcgacttt agaggattaa gtccatttat cgatgaagaa ttttcagaag
      181 aatggcgcct attcttgcca agagaagaag gaagccaacg cggtgtggta tttggcgaat
      241 ttcaattagg aaatatgatt gtcggtacga tcgaagaaac gggcagtttg gtgacagagt
      301 atcaaaaagg agagttagtc tgcacttatg gacccattcg tgaaacgctg attacaaacg
      361 gtgttgataa ttataaacta cgtaaacttg atcggttgga tcaatggaaa aatgcagtat
      421 gttacgatcc tgctcaattc gctttaggtg gtatccggga agcacatgtt cgagcaggtg
      481 atgctgtagc aatcttcggt ttaggcgcta tcgggcaaat tgcgattcaa ttagctaaaa
      541 aagcaggtgc tactaccatc gttgggatcg atcccatagc gcagcgtaga gaattagcag
      601 aaaaatacgg tgccgattac acgctagatc ctatgaaagc agatgtcggt tttcgtctaa
      661 aggaattgac agataaacgt ggagtagatt cagttattga aacgagtggc agtgctgccg
      721 cattacaagc ttctttaaga gggatcggct atggcgggat catttcttat gttgcctttg
      781 ctaaaccttt tccagctggg tttaatcttg gtcgcgaagc acatttcaat aatgcaaaaa
      841 tcgtctttgc tcgagcatct agtgaaccaa acccagatta cccaagatgg aaccgtaaac
      901 gaatcgagga cacatgttgg gaattattga tgaacggtta cttggattgt gaagaaatca
      961 ttgatccagt tgtgccattt gcagaaagcg cagaagctta catgcgttat gtagaccagc
     1021 acccagaaga aagtatcaaa atgggcgttc aagtccgagg aggagaacaa tgaaattcgg
     1081 tacgcaagat caagcatttt tcccaagaga cattttagaa aaatttagat ttatcaaaga
     1141 ctgtggattt gaagaatacg aaatcgacgg taagttgtta atagaaaata tcgacacagt
     1201 caaacaggca atcaaagaaa cagggatccc agtagtgaca gcttgtaacg gctacgatgg
     1261 gtggatcggt gactttatcg aagaacggcg tcttaatggt gtacgtcaaa ttgctgagat
     1321 tttgcgtgca ctaagcgagg ttggaggcaa aggaatcgtt atcccagcag cttggggaat
     1381 gttcacttat cgattaccgc cgatggtttc accccgaagc aaagaaggag actataaagc
     1441 agtcagcgaa tcacttcgtt atttagataa agtagccgaa gaaacaggta cggttattta
     1501 tttggagcct ttgaaccgat accaagacca tatgatcaat ttgttagcgg atgcgcgtca
     1561 ttacattgac tctaatcaat tgaaacatgt gcagatcatc gctgattttt atcatatgaa
     1621 tattgaagaa gatgatattt caaaagcatt gactacgcaa aaagagtatg tgggtcatat
     1681 ccatttagca gataatcatc gctaccaacc aggatctggc gcaattgatt ttactcgcca
     1741 ttttcaaacg ctacttcaaa atgagtatga aggttatatg gtatatgaat gtcgtttgcg
     1801 aggagaaaat gaagtgaaaa tttatcagga atcattgcaa tacttgaaaa actgtctgga
     1861 aacagcgcgt catttaaatg atttagagaa agaatagaga aagaataaca ataagtaaat
     1921 aagcaaagga gagagaaata aatgaagaaa gtagcaatca taggcgctgg acaagtagca
     1981 gaaaaagtac atgctgctta ttatcaaaaa agatcagatg tcttagaatt ggccgctgtg
     2041 gttgatccag tcagccagcg agcagaagac ttttgccgac gtacaggatt ttctcatgcg
     2101 tatacatcag ttacacaaat gctgaatgag atttctcccg atatcgtgag tgtctgtaca
     2161 cccaatcgct ttcacttcga acatgtattg gctgccctcg aagctaatgc ttcagtgatg
     2221 tgcgagaaac caccagctat gacagctgat caagcaagaa agatggcaga gacagccgat
     2281 aaaaacgggc agattttggc ttatggattc cagcatcgtt tttctgacga agcaacaatg
     2341 atcaagaatc atcttgatgt tctcggggat atttattacg tagaagcgaa tgcgcttcgt
     2401 cggtctggtg ttccaggctg gggaacgttt atcgacaaag aacttcaagg aggcgggcct
     2461 ttgatcgatt atggcattca catgcttgat acagcgatgt atattactgg atttcctaaa
     2521 gtaaaaagcg ttcgggcgta cagcttccaa aaaattggta cgaaaaaaaa ttcagggaaa
     2581 ttcgggaaat gggatccaag caaattcaca gtagaagatg caatttttgg cacactggaa
     2641 ttagaaaacg gcaagatcat ccaactgaat acttcgtttg ctttgaatat cagagaagac
     2701 aaactgaatg tggctttttg tggcgatcag gcaggtgcta atctgtatcc aggcgaacta
     2761 ttcgaagatg tagatggaaa gctggtttcc ttacaaaaga ccacgccagt taatgaccca
     2821 catgaactaa gtatggctca ttttgtgaat cgggcgctcg gtgaccagac tcagatgatt
     2881 gcagatggct ggcaaggata tgtcatccag aatctggtgg aaaatttata tcgttcggca
     2941 gaaacaggag aagttattac cttatgaaag tagaagaaaa acagtttgat ttagagtata
     3001 tcaataagta tgcgagtcag atgaccattg gaaatggatt ttttggtatc agaggaagcc
     3061 aggaggaaga ctatcggaat caagtaaggg gaatgtttgc agctggtgta tataaccgac
     3121 ctttaggagc agaatcagcc gagttagtga atctcccaga tgtcatcaga tgggaaataa
     3181 aaatcgacgg tgaacatttt tctcttgaga cgatgaaagt tcttgcatac gagcgggcac
     3241 tagatttaga aatgggagag cttaaacgct atgcgctcat tgaaactgct caaggaaaaa
     3301 aagtggaagt cattacttgt cgtgttgctg atcaaaataa tctccatcag gcagccctga
     3361 aagtggtcat caagccgttg accgaagact gccaagtaga tgtgaaaacg ggtatcgatg
     3421 gacagcaaac caattttggt acacagcagt tggttgaaaa agaactgcga gtatttggtg
     3481 acgatttgat cagtgcatcc taccagacaa cagaaagtaa aattgatatt gggattgcag
     3541 ctaaattcaa taaagcaggt gtattccaag caaaaaatcg ccggatcacc gcttcctttt
     3601 cagaaaagat cagaaaaaat acgtccttta cgctggagaa gatcatcgtg ctctctacct
     3661 ctcttcagca aacagacccg tttaagaaaa gtattgccca agcccagaaa agcgttagtt
     3721 atacagaaat tttagagcag tcgatgtctt attggacaga tttttggcat cagcatcgga
     3781 taacgatcca aggcgattct tctttcgatc agttagcgat ggacttcgct gcttaccatt
     3841 tacagattat gactccccgc aatgatccaa gtgttagtgt aggggcgaag ggactaactg
     3901 gcgaaggtta taaagggcat gttttctggg atacggaaat ttttatcctg cctttctttt
     3961 tatatcagtt tcctgaagta gcaaagaatt tgctgctgta ccgttatgat cgattagaag
     4021 gcgcacggca aaaagcaaga gattatcact atcaaggtgc acttttccca tgggaaagcg
     4081 cacacactgg tcaggaagag acacccaaat ttgctgcatt gaatatcaaa accggaacaa
     4141 gacaagaagt tgcttcagct gccgcagaac atcatatcag tgcagacctt gcttatgccg
     4201 ttcaagagta tgtccaagca acagaggatc aatcgttttt agaaacaaag ggaaaaacgc
     4261 tcattcatga aacagcagct ttttggctga gcagagctgt aaaagtcgat gatcatttag
     4321 agatacacag agtcattggt ccagatgaat acacagaata tatcgacaat aatgcatata
     4381 cgaactatct ggcgcattat aatgtggcct tagctgttaa atatcaagct ggcgatccgc
     4441 tttttttgaa agaatgtcaa aattttttag ataagttata tttgccagtg cctaacaaag
     4501 aacagctgat tccgcaagat gacacgtatc tcagtaagcc agttattgat ttgaccactt
     4561 acaaagaacg tcctggcagc cagtctattt tacttgatta tgcccgtagc gaagtaaatg
     4621 aactccaagt cagcaagcaa gcagatcttg tcatgctgtt atacttatta cctgagtatt
     4681 tttctcaacc aatcgtttct aagaatttgc attactatga aaacaaaaca atccacgact
     4741 catcgttgag taaagcgatt cacgccatag aggcttgccg tatccaagaa atggaatggg
     4801 cgtatcattt attccaagaa gcgtgcctga ttgatttagg accagatccg cattcatcag
     4861 atgacggaat tcatgctgct tcattaggag cactatggct agcagttata tttggttttt
     4921 caggtattac agtaacgaat ggaactcttt ctatttctcc aagacttcct tcagcatggg
     4981 aaaaaatttc ttttcctttt atttggaaaa atcacaggtt gctatttgtt ttgacaaaaa
     5041 aacaaatagt aataaaaaaa gagacgaaag aaacattacc tatttttgtc aatggagacc
     5101 aataccagct ggaaaaagaa ctgatcttgg aacggagggg aaaataatgc gtattctgat
     5161 tgcgccagat tctttcaaag aaagtctgac agccttagag gcagcacaag ccattcaatc
     5221 aggttttcaa gaagtttttg ttcaggctga atatgatctg ctacctatag gcgatggagg
     5281 agaaggaacg gtttcatcat tagcagacag tttaaggttg aagcggcaaa cagcagtcgt
     5341 tactaatgca tttggcaaaa acaaagaaat ccagtacgct tcagacggtc aaactgcgat
     5401 ttttgaaatg gcagaaatcg tcggattgaa agatgtccca ctaaaccaac aacagccact
     5461 taggttatcc acgcgaggag taggagaact gatacgacaa ttgttgcaac aaggtgttcg
     5521 acacatcatc atcggcgttg gcggaagtag taccaacgat ggcggcatcg gcatggcaga
     5581 aggtctaggt tatgcttttt acgatcaaga taatcaaaaa gtagaagcac tgggagaaaa
     5641 tctaggtaaa attgctacag ttgataaaac gaacgtgcct attgagctga ttaaagccaa
     5701 aatcactgtc attactgatg ttactaatct tttatgcgga aaagaaggcg cttcttttgt
     5761 tttcggacca caaaaaggct tgaaagaaaa tgaattagca gtggctgacc aagccatgca
     5821 tgatttttat atgagatttg cccctcaagt aatcgaggca tcgggcagta gtgctggtgg
     5881 tggaatgggt gcaggactgt tagcatttgc ttcagggaaa atagtctctg gcatcgacta
     5941 cgtattggat ttactggagt tcgataaacg ggtacagcaa gcagacctcg taatcgtagg
     6001 cgaaggacgt ctagatggac aaagtctaaa aggaaaagcg ccagttggga ttgctagaag
     6061 agtaccagag ggcgttcctg tgatagctat ttgcggaagt gttggtaaag gaagcgaaaa
     6121 agcagcagat gttggcattt cggccgtttt tccgatcctt cctgcagctg tgcctttaca
     6181 agaagcttta cagcaagcca ctgataatct tcatcgcaca gctaaaaaca tcgcatcttt
     6241 acttcaagtc gttcattttc aaggaggtaa tcgatgaaaa tcaaagcagc actattcgat
     6301 ttagatggcg ttcttgtaga cacagctcgt tatcattacg aagcgtggct ggttttagct
     6361 aaccagctgt ccattccatt cactgagaaa gaaaacgagc aactgaaagg aatatccaga
     6421 accgagtcac tagagcgatt gctgtcattt ggaaaaatgg aacagaaatt ctcagaaaaa
     6481 gagaaatcag cctttgctga gcaaaaaaac aatctatatc ttcaagcgat tcaaaaaatg
     6541 gatgaaacat ccgttttgcc tggtgcaata gctgttcttg agtacttgaa aaaaacaaac
     6601 atcaagatcg gattgggatc agcaagcaaa aatgcacgcc tcattttaga aaaaacgaat
     6661 ttgacaagtt attttgatgt ccttattgac ggcacgcaag tatcaaaagc aaaacctgat
     6721 ccagaagttt ttctcaaagg agcacagcaa ttgaatgtac cgccaaatgc atgcttggtc
     6781 attgaagatt cagaagctgg ttgtcaagca gctttggctg gtaacatgca tgtattggga
     6841 ataggtgaaa acataaatct tccttctgct gaatatgtga ttcctgattt aaccgttttt
     6901 gatcaagtac gttcgttttg gcatttatca gaagccgtat cacgtatata atagtttccg
     6961 ttttttctgt cacaagcaaa acgttatcct ccttttggaa atcaaggtat gatgagaaaa
     7021 aagggggatt ttatatgaca aaaaaagcag ttttcgcagg cggatgcttt tggtgtatgg
     7081 tcaaaccatt tgaagagcag ccagggatca tttctgtgac atccggttat actggcgggc
     7141 atgtaccaaa tccaacttat gaacaagtaa caacaggcac aacagggcat acagaagcag
     7201 tagagatcga atatgatcca gaagttattt cttatgatca gttggtggaa atctattggc
     7261 agcaaaccga tccgactgac gcattaggac agttcgctga tcgcggtgat tcttatcgtc
     7321 cagtgatcta ctataatgat gaagaagaac gaaatattgc agagcgttca aaacaagcat
     7381 tgcaagacag cgggcgattc gataagccaa tcgtgacaaa aatcgaacca agaagcgaat
     7441 tttatcctgc agaagattac catcaggatt attacaagaa aaacaagctc cattacaatc
     7501 tttatcgaga aggttctgga cgtgcaggtt tccttcgtcg acattggggc gtcaagaaat
     7561 aagaagccga aaaagctact tatggaacac cgtatatcag aggttgtaaa accagcattt
     7621 tgagctaata ctgagggtca gctgattgaa caccatttag tggagaggtt gtgaacctgt
     7681 cgcctagctc caagtcacaa ggatttggta agagaacact atatatcaga ggttgtgaca
     7741 agtcgtttgt cacaaccttt tttcaatacc taagatgaag gacaatcagc aacgcactgc
     7801 tcaaaaaagg aatgaaaggc agcttttcca actttttttt atgacagata aagtaaacag
     7861 aaaaaacgga tattccaaac agactagcaa gaaacaagat ctgaagcaac tcttctagag
     7921 acaagccacc actccaagaa agcatcaaca gagtatcgcc agctccgaga taggattgac
     7981 catagcgaag gcacacaccg cctacgataa acgttaacag cacactttgc cattggaaga
     8041 cgccggcctg caaccaaaaa atccataaaa ttgcccaaga acagaatagc gtgtgagaat
     8101 ggaccatgaa gtaaaataaa tccatcaacg aaaatagcaa agccgaaagc atccatatga
     8161 acgtcgaaaa atttcttgcg gaagaaaaag taaagaacca ggcaaaaaga ccaccacata
     8221 aggcttctgc caacaaatag tagaagggca accgacacct gcaatagcgg cagcgaaatc
     8281 gttggacgat gatcgagaga atcggaatca attcatacca agaaagcgaa cgacagcatt
     8341 tgacacaatg agatctagga taaataatag agtgacctaa cggaatccgc tgggcggtta
     8401 aacataaaaa tgatccgata cagcaaccaa tgataaaata aagtaacacg ccatctcccc
     8461 cttttcaaaa ccaagatacg caagcttaaa ctaaattttt gtacatacat aaaatttgag
     8521 gtgaaaaaat gaacgtttca atcggattag atatcggaac cacacaaacc aaagcagttg
     8581 ctttcacaga tgaagcccaa gaagcagcat ccgcttattt tcgctaccct ttgatccaag
     8641 aaacacaagg gatggcagaa caagatcagg aattgatctt tcaaggtgtc tgcaccgtta
     8701 taaaagaagt aattaaacag ttgccttcag acacaatcat tcaatttgtc tccttctcct
     8761 ctgcgatgca tggcctgata ttgttggatg aagagaaaac accgttatct cgaatgatca
     8821 cttgggcaga taaccgagca gcggacgaag cagaaaaact gaaatatacc gtgcttggca
     8881 aagaatttta tcggcagact gggacgccga ttcatccaat gacaccattg aacaaaatca
     8941 agtggctgca aaacagccaa cctgaattat tcaaaaaaac tgcttatttt gtcggtatca
     9001 aagactatat cttttataga ttgtttggcg aactgatcag cgactacgga acagcttcag
     9061 gaactggata ttttgatatc catgaattcc aatgggcaga agaagtgctt gactacttga
     9121 acattcgaat agagcaattg ccgcaagtct ttccttccac ctatcaagta acaggtctac
     9181 catcaaaaac agcagcagaa ctaggtttgc caaatgatat atcatttgtt ttgggagggg
     9241 cagacgggcc gctttctaat ctagggttag gtgcttttgg tgatacgatc gccgctttga
     9301 ccgtaggaac gagcggagca cttcgtttta ttactactca gcctcagttc catccagaaa
     9361 tggagacgtt ttgttatgtt ctggatcaga aacattgggt agtgggagga gcgactagca
     9421 atggtgctgg catctttgat tgggcagggc atacattttt acaggaagtc gttcaatctg
     9481 ctaaagaaaa aggagaaaat ccatatgatg cactgctttc ggctatcgct tttgtgccag
     9541 cgggtgccaa tggtctgatt tttcatccct atttacttgg tgagcgagca ccgctttggg
     9601 acgcagatgc gtttggcagt tttatcggtt tacagcggca acatacagaa aaagaaatga
     9661 tgaaggcgat tctagaaggg atctgtctca acctttatcg gattttaaaa ggagtttgcc
     9721 aggaaaatca gctcacagag atccgtgcta caggcgggtt tgctcaatct ccgatttttc
     9781 gtcagatgat ggctgatgta ctgggttacc cgcttgtttt ccctactgtc acagaagcat
     9841 ccgcattagg agctgtgatt ttaggctgga gaagcatcgg aaagatccaa agtatcagtg
     9901 aagcacagga attgatagaa ttgaaagagc aagttggagt acaagaaact gatcataaaa
     9961 aatatcagaa aatctatcca ttatttgttt cgacacaaaa gcatttagca cagacttatc
    10021 agcaatttgc tcaactgcga gaagaattag ctgattctga aaaatgattg gctttacgga
    10081 taaaaaagcg aactgaaaat cgttctcagg taaatgattt tgttgtactt ttcattgaaa
    10141 gtctactatt attttaagtg taagaaaatg ttaggaggca agaaagatga aattccaaca
    10201 aaccaaagaa gtattaaatc aattagttgc agatctaagc caaatgtctg ttgttgttca
    10261 ccaaacacat tggtacatga gaggaccagg tttccttaca ttgcacccaa tgatggatga
    10321 gttcatggat gatttgaacg ctcagttaga tgaagtatcc gaacgcttga tcacattaga
    10381 cggttcacct tattctacat tgcgtgaatt tgcagataac acaaaaatcc ctgatgaaat
    10441 tggccgctgg gatcgtacta ttccagaacg tttggaaaca cttgttgcag gctatcgtta
    10501 tctagcagat ctttaccaaa aaggaatcga agtttctgga gaagaaggcg acgatccaac
    10561 acaagatatc tttatcgaat tcaaaacagc cacagaaaaa cgtatctgga tgatccaagc
    10621 tcatttgggc aaagcgccag agatcgacgc gtaaaaactg ttgttgaact tgaccgaaaa
    10681 agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacgtc aaaatagttc
    10741 ctcatatttt gccgtttttt g
//
LOCUS       DACBZN010000094        10646 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.094, whole genome shotgun sequence.
ACCESSION   DACBZN010000094 DACBZN010000000
VERSION     DACBZN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.094"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            556..756
                     /locus_tag="IVD64_002237"
     CDS             556..756
                     /locus_tag="IVD64_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6149472.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1324..1887
                     /locus_tag="IVD64_002238"
     CDS             1324..1887
                     /locus_tag="IVD64_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149473.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1948..2292)
                     /locus_tag="IVD64_002239"
     CDS             complement(1948..2292)
                     /locus_tag="IVD64_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149474.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2389..2634)
                     /locus_tag="IVD64_002240"
     CDS             complement(2389..2634)
                     /locus_tag="IVD64_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6149475.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2704..2958)
                     /locus_tag="IVD64_002241"
     CDS             complement(2704..2958)
                     /locus_tag="IVD64_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149476.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3122..3445)
                     /locus_tag="IVD64_002242"
     CDS             complement(3122..3445)
                     /locus_tag="IVD64_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149477.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3632..3705
                     /locus_tag="IVD64_002243"
     tRNA            3632..3705
                     /locus_tag="IVD64_002243"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Ile,seq:cat)
     gene            complement(4090..4587)
                     /locus_tag="IVD64_002244"
     CDS             complement(4090..4587)
                     /locus_tag="IVD64_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6149478.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4627..5436)
                     /locus_tag="IVD64_002245"
     CDS             complement(4627..5436)
                     /locus_tag="IVD64_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6149479.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5770..6627
                     /locus_tag="IVD64_002246"
     CDS             5770..6627
                     /locus_tag="IVD64_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6149480.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6620..7522
                     /locus_tag="IVD64_002247"
     CDS             6620..7522
                     /locus_tag="IVD64_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6149481.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7555..7905
                     /locus_tag="IVD64_002248"
     CDS             7555..7905
                     /locus_tag="IVD64_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6149482.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7939..8877
                     /locus_tag="IVD64_002249"
     CDS             7939..8877
                     /locus_tag="IVD64_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6149483.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8955..9848
                     /locus_tag="IVD64_002250"
     CDS             8955..9848
                     /locus_tag="IVD64_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6149484.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9886..10449
                     /gene="amaP"
                     /locus_tag="IVD64_002251"
     CDS             9886..10449
                     /gene="amaP"
                     /locus_tag="IVD64_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6149485.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10464..>10646
                     /locus_tag="IVD64_002252"
     CDS             10464..>10646
                     /locus_tag="IVD64_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6149486.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFK"
BASE COUNT         3706 a         1791 c         1982 g         3167 t
ORIGIN      
        1 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
       61 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
      121 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
      181 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
      241 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
      301 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
      361 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
      421 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
      481 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
      541 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
      601 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
      661 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
      721 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
      781 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
      841 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
      901 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
      961 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
     1021 attaatgaaa aagtaaaaaa gaatatattc taggaagagg gcggcacatc taaattttta
     1081 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
     1141 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
     1201 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aataattttt
     1261 ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt tttaaaaata
     1321 actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga atatttagaa
     1381 attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag
     1441 ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca
     1501 ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca
     1561 gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg
     1621 ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga
     1681 agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca
     1741 gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa
     1801 gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac
     1861 cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag
     1921 aggagataga aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt
     1981 tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc
     2041 tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca
     2101 ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg
     2161 gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca
     2221 gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag
     2281 ctttgaaatc aatttttcat gccataatca catagaaatg tctcctttat atatagattg
     2341 ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt
     2401 gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat
     2461 atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa
     2521 taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa
     2581 gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc
     2641 acctttcatt atagtagaat agttataatg actatagaaa gctacacatg caatcaatag
     2701 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
     2761 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
     2821 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
     2881 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
     2941 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
     3001 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
     3061 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
     3121 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
     3181 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
     3241 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
     3301 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
     3361 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
     3421 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
     3481 taagcaaata aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata
     3541 acaatattat tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg
     3601 aaaagtaaca aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga
     3661 cggctcataa ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa
     3721 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac
     3781 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
     3841 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
     3901 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
     3961 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
     4021 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
     4081 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
     4141 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
     4201 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
     4261 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
     4321 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
     4381 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
     4441 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
     4501 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
     4561 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
     4621 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
     4681 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
     4741 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     4801 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     4861 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     4921 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     4981 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     5041 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     5101 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     5161 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     5221 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     5281 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     5341 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     5401 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     5461 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     5521 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     5581 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     5641 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     5701 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     5761 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     5821 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     5881 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     5941 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     6001 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     6061 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     6121 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     6181 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     6241 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     6301 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     6361 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     6421 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     6481 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     6541 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     6601 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     6661 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     6721 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     6781 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     6841 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     6901 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     6961 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     7021 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     7081 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     7141 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     7201 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     7261 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     7321 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     7381 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     7441 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     7501 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     7561 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     7621 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     7681 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     7741 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     7801 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     7861 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     7921 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     7981 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     8041 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     8101 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     8161 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     8221 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     8281 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     8341 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     8401 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     8461 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     8521 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     8581 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     8641 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     8701 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     8761 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     8821 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     8881 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     8941 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     9001 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     9061 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     9121 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     9181 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     9241 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     9301 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     9361 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     9421 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     9481 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     9541 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     9601 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     9661 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     9721 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     9781 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     9841 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     9901 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     9961 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
    10021 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
    10081 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
    10141 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
    10201 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
    10261 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
    10321 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
    10381 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
    10441 gtagaatagg ggggggacca aaaatgaaag aactattttc gacatacaag ctccctatca
    10501 tctttggggt acttggattg atattagccg ttttatggat caccgtgggc tttttcaaga
    10561 ccttattgat cttgatcatg gcagcgatcg gcgtcgctat cggtttttat ctgaaacaaa
    10621 cgaaaatctt agatcactat tttaaa
//
LOCUS       DACBZN010000095        10500 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.095, whole genome shotgun sequence.
ACCESSION   DACBZN010000095 DACBZN010000000
VERSION     DACBZN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.095"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(210..962)
                     /gene="xth"
                     /locus_tag="IVD64_002253"
     CDS             complement(210..962)
                     /gene="xth"
                     /locus_tag="IVD64_002253"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP6149487.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1075..1995
                     /locus_tag="IVD64_002254"
     CDS             1075..1995
                     /locus_tag="IVD64_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6149488.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2068..3003
                     /gene="murB"
                     /locus_tag="IVD64_002255"
     CDS             2068..3003
                     /gene="murB"
                     /locus_tag="IVD64_002255"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP6149489.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3301..4779
                     /locus_tag="IVD64_002256"
     CDS             3301..4779
                     /locus_tag="IVD64_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6149490.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5056..7116
                     /locus_tag="IVD64_002257"
     CDS             5056..7116
                     /locus_tag="IVD64_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149491.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7199..7768
                     /locus_tag="IVD64_002258"
     CDS             7199..7768
                     /locus_tag="IVD64_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6149492.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7943..8749)
                     /locus_tag="IVD64_002259"
     CDS             complement(7943..8749)
                     /locus_tag="IVD64_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149493.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8887..10401
                     /locus_tag="IVD64_002260"
     CDS             8887..10401
                     /locus_tag="IVD64_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP6149494.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3724 a         1750 c         2068 g         2958 t
ORIGIN      
        1 ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt cttattactt
       61 gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc agtttctcgg
      121 tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt tcttaatact
      181 cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac tggacaatga
      241 tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc taaatctttg
      301 ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt gaatcggtag
      361 ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt atcgataaac
      421 ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc agcatttttt
      481 tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag atccccacac
      541 acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc ttcttcccat
      601 tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta acaggttaca
      661 agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg gatatcgata
      721 cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt gccggaatag
      781 ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa atcaatttgt
      841 cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc atgaaaaact
      901 tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga gataaatttc
      961 aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt gtacctcatt
     1021 ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa gtagatgaag
     1081 attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac ttatgcgaaa
     1141 aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa agaaagaatt
     1201 caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt agaagaaaaa
     1261 atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc caaaaaatgt
     1321 ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct aaatgaagtt
     1381 cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg tttcaaccgg
     1441 cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt gatccacctg
     1501 caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga tttgttttta
     1561 catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac aaaataccag
     1621 ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac acaaaatcaa
     1681 acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata tcaagtaaca
     1741 agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca agatgctgat
     1801 ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg cgggtttgag
     1861 ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac agaattgctt
     1921 aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa agatcactac
     1981 cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa taatgaggac
     2041 gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga aagcttagcc
     2101 acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa aacaggtgga
     2161 ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat catcgattat
     2221 tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct gatcgttcaa
     2281 gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat tcatgtcaaa
     2341 ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta tgcagctttg
     2401 gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt cggtggagcg
     2461 gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc tgaagtggat
     2521 cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt attttcttat
     2581 cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt cagtttgcaa
     2641 acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt gcgtcaatca
     2701 aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt aggacactat
     2761 acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg tgcacaagtc
     2821 tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac agattatatt
     2881 gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc tttagaaaca
     2941 gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag tttagccaat
     3001 taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc ctgctctttt
     3061 ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata aaacaaaaaa
     3121 tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa aaaagataaa
     3181 aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt ataggtgaaa
     3241 agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga ggtgtaggat
     3301 atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct agctagatat
     3361 ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac attaggtttg
     3421 tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac tgaattcaaa
     3481 acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg
     3541 acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc tcgactgtta
     3601 acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca ttttttaagc
     3661 atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca gcatcaaata
     3721 aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt attatttggg
     3781 gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt catgaatgaa
     3841 gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa
     3901 cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg gaatcaacca
     3961 atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat gaagatcatc
     4021 aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat tgatgagatt
     4081 tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg cgcatttgga
     4141 cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc agtggaattg
     4201 attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata tatacgaaaa
     4261 gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg
     4321 actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc aatattcagt
     4381 acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa agatgaacgt
     4441 ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg tccggtatca
     4501 tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc ttataccgat
     4561 tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga gagtcgaatc
     4621 acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa taaagaacag
     4681 ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa taccatcatc
     4741 agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat ggcagctttg
     4801 tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact agatataact
     4861 agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat tattccgatt
     4921 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
     4981 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
     5041 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
     5101 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
     5161 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
     5221 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
     5281 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
     5341 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
     5401 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
     5461 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
     5521 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
     5581 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
     5641 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
     5701 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
     5761 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
     5821 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
     5881 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     5941 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     6001 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     6061 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     6121 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     6181 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     6241 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     6301 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     6361 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     6421 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     6481 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     6541 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     6601 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     6661 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     6721 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     6781 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     6841 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     6901 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     6961 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     7021 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     7081 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     7141 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     7201 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     7261 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     7321 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     7381 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     7441 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     7501 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     7561 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     7621 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     7681 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     7741 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     7801 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     7861 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     7921 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     7981 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     8041 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     8101 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     8161 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     8221 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     8281 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     8341 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     8401 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     8461 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     8521 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     8581 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     8641 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     8701 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     8761 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     8821 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     8881 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     8941 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     9001 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     9061 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     9121 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     9181 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     9241 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     9301 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     9361 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     9421 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     9481 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     9541 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     9601 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     9661 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     9721 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     9781 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     9841 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     9901 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     9961 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
    10021 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
    10081 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
    10141 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
    10201 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
    10261 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
    10321 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
    10381 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
    10441 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
//
LOCUS       DACBZN010000096        10362 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.096, whole genome shotgun sequence.
ACCESSION   DACBZN010000096 DACBZN010000000
VERSION     DACBZN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.096"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            174..323
                     /locus_tag="IVD64_002261"
     CDS             174..323
                     /locus_tag="IVD64_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP6149495.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            334..1848
                     /gene="dltA"
                     /locus_tag="IVD64_002262"
     CDS             334..1848
                     /gene="dltA"
                     /locus_tag="IVD64_002262"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP6149496.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1845..3050
                     /gene="dltB"
                     /locus_tag="IVD64_002263"
     CDS             1845..3050
                     /gene="dltB"
                     /locus_tag="IVD64_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP6149497.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3095..3328
                     /gene="dltC"
                     /locus_tag="IVD64_002264"
     CDS             3095..3328
                     /gene="dltC"
                     /locus_tag="IVD64_002264"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP6149498.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3331..4599
                     /gene="dltD"
                     /locus_tag="IVD64_002265"
     CDS             3331..4599
                     /gene="dltD"
                     /locus_tag="IVD64_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP6149499.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4740..5822
                     /locus_tag="IVD64_002266"
     CDS             4740..5822
                     /locus_tag="IVD64_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP6149500.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5822..6625
                     /locus_tag="IVD64_002267"
     CDS             5822..6625
                     /locus_tag="IVD64_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6149501.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6695..7435
                     /locus_tag="IVD64_002268"
     CDS             6695..7435
                     /locus_tag="IVD64_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6149502.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7446..8450
                     /locus_tag="IVD64_002269"
     CDS             7446..8450
                     /locus_tag="IVD64_002269"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP6149503.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8495..10339)
                     /locus_tag="IVD64_002270"
     CDS             complement(8495..10339)
                     /locus_tag="IVD64_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149504.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3338 a         1708 c         2090 g         3226 t
ORIGIN      
        1 tttttaaaag tacgagaaat tataaagaaa aaagcagtga tttgatgtgc tatttttata
       61 aaagctatgt gacaaaactg ttagtaaaaa aacggtttct gtaagctaga cacatgtttc
      121 tttttgtcaa aattagttac actttgtgtg aggttatatg aggagggaat ccaatgaaca
      181 aatggcgttc taatccaaaa ttgaattatt ggggaaaatt catcattcga acaatttttt
      241 atagtgcagt actgctgttc ctgatttatc tgtatcacta taaaaatatt caaggaggaa
      301 cttttatcta taacgaattt taggaggaag agtatggaaa tcaaaacgat tattgaagca
      361 atcgatgatt ggggaataaa tgaacctgat cgagttgctt atcaggcgga tgagacgcat
      421 acttttggtg aattgaaaca agcatcagac gcacttgctt attatttaga aaaaaaggta
      481 gaaggcgatg gaccaattgt cgtttttggc aatttggagt ttgaaatgat tgtctcattt
      541 ctaggggttg tcaaatctgg ccatgcctat ttgccgattg aagagcatac acccaaggag
      601 cgtatcctat cgatctttcg ggtggctaaa ccatctatgg tcatcagtat cggggactgg
      661 atagaagata ttccttcggt tcctgtcata acaaaaaaag aatttcaaga aatcaagaag
      721 ataccagtag gattttatcc cagaaatagc gtaaaaggta cagaaaattt ttatattatt
      781 tttacttctg gtactactgg agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc
      841 agctttgttc aatggacgat ggaagacttt gggatcaagc ctggtatgca tttcttagcc
      901 caagcgccat tttcttttga tttatcggta ttttctattt atcctgcatt agtttctggc
      961 ggaatgctga agccgttaaa taaagcagtt gttcaagact ttcgtcaatt attcgcgaca
     1021 ttgccagcct tgaagttgaa tgtgtgggtt tctactccat catttatgga tatttgcttg
     1081 atggaaccta cattcaatgg agaaaatgtg ccggaattgg acatgttttt attttgcgga
     1141 gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc gattcccaaa tgcacggata
     1201 tataatactt acggtccaac agaagcaact gtagctattt caagtattca gattgaccag
     1261 catgtactag atagttatga ccgactaccg attggctacg taaaaaaaga cacgaaagtt
     1321 tctattgtcc aagaaggtaa acctgtttca aaaggagaaa cgggtgaaat cattattgca
     1381 ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa agacagctgc tgcgttcttt
     1441 tgtcaaaatg acttagcaag ttaccataca ggggatgcag gtagactaga tgaatatggg
     1501 cttttatttt atgaaggacg tatggatcag caggtcaaat tacatggata ccggattgaa
     1561 ctcggggata tcgaacatta tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta
     1621 ccaaaatacc aaggaacaaa agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa
     1681 acgaaacagc cggattttca gttgactaaa tctatcaaag aacaattact agaagttgta
     1741 atggattata tgattcctca gaagttccat tatgtcgatc tgctgcctca gacagttaat
     1801 ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc ctgaatgatg ttacctcata
     1861 tgatccctta tgcagagccg ttttattttt ttcttttagc cattgcgctt gtaccgatcg
     1921 ttctatcttt attgatatgg gaaaaaagat taccagttta tcaatcagca gtgacacttt
     1981 tctttttatt tgtcagcttt ggaggtaatt attggcaaca gggagcggcg ctgattgggt
     2041 atgttttgtg gcagacgata cttgtatggg gctattttgt ttatcggcaa aggaaaaatc
     2101 agacaggatg gttttttgcc gccgttattt tagcgataat tccactgttt ttaacgaaag
     2161 tgactccttt ttttgaacat ggaaaagcat cgttacttgg ttttttagga atctcttatt
     2221 taacctttaa atctgtacaa atgatcatgg aaataagaga tggcatgatc aaaacatata
     2281 agccatttcg ttatatccag tttctgctgt tctttccaac aatctcttct ggaccaatcg
     2341 atcgctatag gagatttgaa aaagatcagc tccatccgcc tgttcgtgat aaatatgttg
     2401 atcttttggg aaaaggcgtc caaaatattt ttgtcggttt tttatataaa ttcattatcg
     2461 gttattattt tggtcaagta ctgcttcctg tagtgggacg cattgcctta cagcatggtg
     2521 gtatttcatg ggcattagta ggttatatgt acgtgtacag tatgtatttg ttttttgatt
     2581 ttgctggcta tagcttattt gcagtcggta caagctacat gatgggatat cagacaccaa
     2641 tcaatttcaa taaacctttt ttatcatgga atatcaaaga attctggaat cgttggcata
     2701 tgacgctgtc cttttggttc cgtgattatg tatatatgcg attgatgttc ttcatgatga
     2761 agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat cggttatttt gctttgtttc
     2821 ttctaatggg tatatggcat ggattgacgt ggtactacat cgtatatgga ctgtatcatg
     2881 cttgtctgat ctgtctgaca gatgcatgga tccgatttaa gaagaagcat aaagagcagt
     2941 tgccttctaa caaatttacg catggcttag ccgtattttt gacatttcaa gcagtatgtt
     3001 ttagcttcct gattttttca ggatttttag ataaactttt ttttagctaa cgaatagtga
     3061 catattaatt tatataacaa aggagaaaag gaaaatggaa gaacaaattt tatcaatctt
     3121 agaagaaata acaggaacag atgaagtaag aaaaaatcgt gatattgatc tatttgatga
     3181 agggttgctt gattctttag catctgttca attattagtc gagctagacg gagagctgga
     3241 tatccaagtc cctgtatctg aattcgagcg agaggattgg agcacaccga ataaaatcat
     3301 tcaacaagca actgcattga agggttgagt atgaaaaaga aactttttgc tattttcggc
     3361 ccggttgttt tagctgttgg cttactgttt gtgtttttta cctcctccta tcaagtagat
     3421 atctctaatc cagaaacgat tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa
     3481 ggagatgcga taaaaaatta tgcattatct gaaaaacaat atattccttt ttttggctca
     3541 agtgaattaa gcagaatcag tccatttcat ccgtctgttc tggcagaaaa ataccagcga
     3601 aactatcgtc catttttgct gggagcaccc gggacacaat cgttaagcca atatatgatg
     3661 atgcgttctg caggagatgc tatgaaaaac aagaaagtcg tctttattat ttctcctcag
     3721 tggttcgtta aaaatggtgt aaaaactgat tactttaata cgtattattc tgaacttcaa
     3781 acatacgact ggttgttttc aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga
     3841 agattattaa ctttctcaaa agtaaaagag aatgatacgt tgacggcgat tctgcaaacg
     3901 atcaaaaaag ggaagctacc tttacctgaa tctttgaatc agctgcgcag ccaatggaac
     3961 atgctgaaga gagaggatga agtattcagc aatattgggt tgaaagatcg acagcaaaaa
     4021 atcgatcatg agtcaaaacg attaccaaaa caataccaag aaacagaatt atccatttta
     4081 gcaaatcaaa ttggagaacg agaaacgacg aataatccat tcgggttgaa aaacgatttt
     4141 tatacgcatc gtatccgagc ccacgagcca gagctcaaac agtcacaaaa aaattgggat
     4201 tatcgttttt cgccagaatt ttctgatttt cagctggtct tggatcaatt agcaaaaaac
     4261 cataacgaag tattgttcat catcccaccg gttaacgaga aatggagcga ttataccgga
     4321 ctatctcaag aaatgttaca aggatttgcc aaaaagatca aatttcaatt aaatagccaa
     4381 ggattcaatc gaatagctga ttttgtcaat caagcgggta ccaactattt tatggaagac
     4441 acgatccatc taggctggaa aggctggttg gctgcagatc aacagatcag accgttttta
     4501 gaagaaaatc atattactgc tagtaagtat catcttgacg atgctttttt tagcaaatct
     4561 tggcaacatc aaataccaga taaactacaa ttaaaataaa gaaaacaggc gagtgttgct
     4621 cgcttgtttt ctttattttt gaatattctg aataaaagat aaaaatacca agtttgcctg
     4681 aacaaaaatc ataagcaatg gtataatcac aatatgaata tagaaatggg ggaaaacatt
     4741 tggatacaaa agaagaacag ctacttattg atttaacttc tgcaaaagga gtacctggaa
     4801 atgaagaaga ggtaagagaa gtattcagag aatatgccaa accattcgct gatgatattt
     4861 tttatgacgg tttaggcagt gtgattgcca aacatggtgc tggcggaggc cccaaggtct
     4921 ttatttctgg acatatggac gaagttggtt ttatggtaac aaaaataacc gaaaaaggat
     4981 tcttggaatt ccaaactctt ggaggttggt gggggcaagt catgcttgct cagcaagttg
     5041 aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg
     5101 ttttaacacc tcaagtaaga aataaacctt atgaaataaa ggatatgttc attgatattg
     5161 gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg tcctggagat atggtcactc
     5221 catatattga atacaaacga atgaatggca gtaaatattt actggcaaaa gcatgggata
     5281 atcgtatcgg gacagctgtt tctctgcgag tattagagaa tctatctaaa gaagcacacc
     5341 caaatgtttt attcgcagga agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa
     5401 caagtacaca ccttgtaaat cccgatattg cttttgcttt agatactggt acagcaggag
     5461 acactccagg aatgacacca aaagaagcag attcgatttt aggaaaaggc ccacagattt
     5521 tgatttttga tgcatctatg atcccgcata aaaaattatt gaattttgtt attgatgtag
     5581 cagaggaaat ggatattcca ttccaatata cagtgatttc tggcggagga actgatgcag
     5641 gacaaatgca tttgacaaga gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt
     5701 atctgcattc ccatacatca gtgattcatg aagatgatta tttgaataca gtgaaactag
     5761 tcacagaatt agtgaaacgg cttgatagtg aaacagttga aaaaattcgg agttatgaat
     5821 aatgaataat agaataccta tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa
     5881 tcaatgggga atcaatactt ggcttgtttt cttatctata atatttacga gcgtcatcca
     5941 gaactttttt gacggtttca gtccttttcc ggaagaaagc atgcagcagc acttattgag
     6001 ttttctagtc gataatttac ttttattcgc attcaactat gcattgtttt acattgcgct
     6061 agtagttgta cggggtggca gaccaaaaag taacctttta ttttcagttt tccaaagaaa
     6121 gtattttggt tcgatacttg gcatcaattt gatcaattca gtcatcaact ggctactcaa
     6181 cagtttaatc tttctgccca tgtttttaat cagcggagtg agtacttata tgcagctgct
     6241 tttcagttca aatgcggtac ctacgggtgt catgccagaa gattttcttg atatcagctt
     6301 tgttttatcg atgataggga tgatttttgt ttcagcactc atcatgagta ttgttggcgg
     6361 tttattccaa tttgctgcat ggacaaagtt cgactatcct gatttgtcgg ttattcaatg
     6421 tttgaaatat ggctggtttt tattgaaaga tagaattggt acgtatattt tgttgcagct
     6481 gtcttttatc ggctggtatt tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt
     6541 cattgtttat gtgaatgtgt cagttgcaga attttatgaa caagcacgga ttgaaaaagg
     6601 aggtccagac gcctatttta cttagcatgt aagcagttta ttcatcaaaa aaataacttt
     6661 tgtggtagga taaatctttg agggggaatg tacgatgaaa ttaactgttt taggatgctt
     6721 aggagcgtat ccttataaaa atcaagggac aacaggttat ttgcttcaat ctgacgggtt
     6781 caatttgttg atcgatgcag gtagtgctac gctgatcaaa ttacaagaac accttgatcc
     6841 acttgatcta gatgctgtga tccttagcca ttatcatcat gatcatattg ctgaccttgg
     6901 tgtattacag tattactggc aacttcatcc taagagaaat ccggagacat tgctttcaat
     6961 ttatggacac acaaaagatt ccattcactt tgaagaattg acgttggaaa atgtctcaaa
     7021 aggacatcct tattttgaag ctgaagaatt aaaaattggt ccttttctga ttacttttat
     7081 ggaaacaatc catccagtag tatgttacgc catgcgtatc gtagaagaaa caacggggaa
     7141 agtattcgta tttacaggtg attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga
     7201 tgctgatttg tttctagcag atacctatct ttttgaagga aatgaacatc atcatgccca
     7261 tttcacttca aaagaagctg gagaacttgc gaaatcagct aacgtaaaga aacttgtact
     7321 tacacaccta cctcagtttg gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc
     7381 aggcaaaggg attgatatag aacttgctgc agttgacaaa gtgtttaaca tttaggagga
     7441 agaacatgat ttttgtacca aatgaaaacc acgacccaag aatcaattta gcaattgaac
     7501 aatatttgct gcaagaaatg cctttagatg aacctatttt gctcttttat atcaatgaac
     7561 cttcaatcat catcggcaga aaccaaaata cgcttgaaga aatcaatcga gattatgtgg
     7621 aagaaaatgg cattcatgtt gtccgtcgct taagtggagg cggagctgtc taccatgatt
     7681 ttggaaacct gaactttagt tttatcatgc ctgatgatgg ggattcattc cgtgactttg
     7741 cgaaagtaac acagccaatc atccaagcac ttcatgaatt gggtgttgct ggggcggaat
     7801 tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg
     7861 ctaccaacgg tcggatgttt gctcatggaa caattatgtt cgactctgat atcaatgaag
     7921 tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc
     7981 gttctcgtgt aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa atgacaacag
     8041 aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa
     8101 aaacgtacga attgactgat gaagattgga aaaaaatcaa tcgaatctct gaggaatatt
     8161 atcgaaattg ggattggaat tacgggaagt ctccagattt caatttctct cgccaaaaac
     8221 gtttttctat cggttcgatt gaagtccatt tgaatgtctc agaaggaatg atcaaagaag
     8281 caaaaatctt tggcgatttc ttcggactag gagatatctc tgacgtggaa aaaaaattgg
     8341 ttggacagaa atatgataaa gcatctctat caaaaatagt cagccaaatt gatgtgaaga
     8401 aatattttgg tgcgatagaa ccagaagaat tgcttacctt actttattga aaataaaaaa
     8461 tagcggaatc cacatatatg gattccgcta ttttttatac aggattcggg taatttcgag
     8521 agagttgaac agtgatccga tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt
     8581 aacgatccgc tacgtctttc atcatcatta agtcaaaacg ttcctgatca tctaattctt
     8641 ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc actatgttcg caacaaatga
     8701 cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt tcgatacagt ggattttcga
     8761 tggcatgagt ttctctaaag gaatgctcta aagcgtggat caaaagttga gccagatcgt
     8821 catcgttgat aggaagttct tcgggaagat ctgcttggaa ggttatctga atattccgtt
     8881 tggcagctaa cttctggtaa cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag
     8941 aacacagcat ttcgaaattt tttgcttctt ctaatatctt ttccacatag tttttagctt
     9001 ttgtttggtc cgtatgcatt aaatcttgca gattctccat ctcttggaca aattccaggc
     9061 gaagtgtatt tacttcttgg atatgctgtc gaagctgctc ataatgttgc tccaacaaat
     9121 catttttgat ttttaagaaa tctacttgtc tccgatcttt gtcggtattt aagatgtgat
     9181 cgattagatt gtagctgata ataacgatta gaatgataat atataaaatc gtagaccaat
     9241 tgatcgttgt ttgatagata cctaatgccg tttggatgta aatcaagcaa tggataatgg
     9301 ctaccaagac gatccaaggt gtacaagcta cataaaaacg gttattctta tatgcttcgc
     9361 caatgcatgc aagtgaagtg actaaagtgc ttataacact agcaatatta gcaatcggca
     9421 ttaattcagg aagttctgca ggagtaaaag ttgcataaag cagaacgccg ataaaaatca
     9481 aaagttggaa cgtacaaaaa attccgtacc attttttata gtaaaacatt cggctccaat
     9541 agtagcagat gatgaaagct gacgttaaga taataaataa atgagataaa aaagaatgaa
     9601 cgaatggttc aaaaagaata ccacttaaaa tatcttctga tacagcttca aatcctaatg
     9661 ccaaagcaaa gcaacttaag agaataagtg agtattcaag acgtttttgt ttatacatca
     9721 agtagatcgt atagaaaaag gtccctatag cgataaaaac agcgattatc atcgtcaata
     9781 cttgcggcac tgattgtgaa taaataaaac taatcaatgg tccagttgtt cctagataaa
     9841 ctttcggtgg cagtccagca tagttctgat aaggagatga gactgtaagg gtcaattttt
     9901 ttccgatata atttgctggt aaatctatca cagccaatga gagtccggga ttgctttctt
     9961 cctgataatc tttccgttgg tataaaatct tttcgcctag atatacagta atccattgat
    10021 ggtttccttt gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa
    10081 tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa
    10141 aatcaggatc atcttttgta tagaaaaccc acgaatcttt tatttctttc atttgattgc
    10201 tgtccgcagg agaaaaaata aaataaaaga aataagcaat tcctgttaaa aataacaatg
    10261 ataatataat gattatgctc cactttatac gtaaataaaa agattctttt ttattatata
    10321 tgttattaaa aaaagacata aaggtattcc tttctaaaac tt
//
LOCUS       DACBZN010000097        10150 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.097, whole genome shotgun sequence.
ACCESSION   DACBZN010000097 DACBZN010000000
VERSION     DACBZN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.097"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            136..456
                     /locus_tag="IVD64_002271"
     CDS             136..456
                     /locus_tag="IVD64_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149505.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            908..1108
                     /locus_tag="IVD64_002272"
     CDS             908..1108
                     /locus_tag="IVD64_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6149506.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1255..4044
                     /locus_tag="IVD64_002273"
     CDS             1255..4044
                     /locus_tag="IVD64_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6149507.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4091..4618
                     /locus_tag="IVD64_002274"
     CDS             4091..4618
                     /locus_tag="IVD64_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6149508.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4639..5829
                     /locus_tag="IVD64_002275"
     CDS             4639..5829
                     /locus_tag="IVD64_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6149509.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5869..7167
                     /gene="asnS"
                     /locus_tag="IVD64_002276"
     CDS             5869..7167
                     /gene="asnS"
                     /locus_tag="IVD64_002276"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP6149510.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8343..9017
                     /locus_tag="IVD64_002277"
     CDS             8343..9017
                     /locus_tag="IVD64_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6149511.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDNIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9014..9355
                     /locus_tag="IVD64_002278"
     CDS             9014..9355
                     /locus_tag="IVD64_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149512.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9385..9744
                     /locus_tag="IVD64_002279"
     CDS             9385..9744
                     /locus_tag="IVD64_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP6149513.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9948..10127
                     /locus_tag="IVD64_002280"
                     /pseudo
     CDS             9948..10127
                     /locus_tag="IVD64_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         3388 a         1812 c         2174 g         2776 t
ORIGIN      
        1 agaggttgtg acgtggtcac gacctctttt tgacgattat gatacaaaaa acatcattta
       61 tgaacaaaat gtgaataacg ggcgttcttg agatatgatt ttaattggga aaaacttatc
      121 gataaggagg tctttatgaa aaaaagactg ttatggtgtt tgctgtttct atccctaagt
      181 ttaacaagtc agccagtttt tgctgaagaa acacagcggt ctcctattac aggcacacgt
      241 ctgtacaaaa atgtttatgt acagacaaca gatcttgatt ctgcgattcc tgaaacagaa
      301 gaacggtctt tgcagccaag atttggttac ttttggtact gtccgacctg tgattatgaa
      361 agttgctggc atttcttaca ttctacagct gcagcttttg cttttactca tactcagaaa
      421 tatgatggac atcatcctgt tatttatgag gtatagatat gagcaaaaaa gaaaaactaa
      481 tctcgttgat cgtattggtt tgccttgttc ttctggtaac tgtcataggc tatcagaaat
      541 accatgagaa catcagacct actaaagaat cttccccagc ctcgaccgtt gaaacagaac
      601 cgataagtag tcagaaagaa taattttttt agtttaaaga agaacatcag agcaaaaaaa
      661 caccaactga acaatatgtc gtttatttta aaaaaagttc ggtttttcac tttatgtaat
      721 cgctgagaaa acttttttta ataaaaaaag tcgaaaaact ctagcttttt tgtaaacggt
      781 atgatatact ataggagtag tttttgttcg gtaatctgtg gtagcttgtt tcaaataaac
      841 atcttccaga tatacaccat tcaagtaatt gcaaaatatg tgttgataca caaggaggat
      901 ttttattatg gaaacaggta cagtaaaatg gtttaactca gaaaaaggat ttggattcat
      961 cactgcagaa aacggaaacg atgtattcgt tcatttctca gctatccaag gcgacggatt
     1021 caaaacttta gaagaaggcc aagcagtaac tttcgatgtt gaagaaggcc aacgcggacc
     1081 tcaagcaaca aacgttacta aagcttaatt aacctatact aaaaaataaa cgcccagaca
     1141 tttgcgtgtc tgggcgtttt tgtctatttt ctttaaaaag cgatgagagt atttttctgc
     1201 actttgtagt ataatttcaa aagaacatta gaggcaggag aagaggaaga tattttgaaa
     1261 ccaagaactt atgcagtcgt tgatatcgaa acgactggga cgaatccaaa agaagatcgg
     1321 atcatccaat ttggctgcgt catgattgaa tcaggtcgga tcacttcacg tttttcgatc
     1381 gatattcatc cagggcggaa gatttcaaaa caaatccagc atttaacagg gatcacgaat
     1441 caacgcgtcc aaaaagcgcc ttatttcgaa gatgtcgccc aaacgattta caatcttttg
     1501 gcagacacca tatttgtcgc gcataatatt tattttgatt ataatttttt gaaccatgaa
     1561 ctcatgcgat gcggacttcc tagtttgaag ataccaggca tcgatacagt agaacttgca
     1621 caaattttct tgccgacgga accaagcttt cgcttagctg atttgtcaga aagccttgga
     1681 tttattcatg agaatcctca tcaggcagat agtgatgccg aagtgacggg tcaacttctt
     1741 ttgctgatcg aagaacggat gagaaaattg ccaatcatca cgttagagca gattgcgcga
     1801 ttaagtagac acacaggtat ggatacgagt cgtttcattt atcatgtgat tgaagaaatg
     1861 aaagaaaaac cagagccact tgatccctca ttagaaatcg tcgatgggct agctttgcag
     1921 aaaaaagaag tggaactatt cacatccgtc cattatggcg agcggacata tccaagaaaa
     1981 aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga acagaacaga
     2041 ctgatgaatg cggtctacga ccatttcaca aaagatgaat caaaagattt gatgatcgaa
     2101 gcagctacag gaatgggtaa aacaatcggt tatctgctgc cattgagtta tttggctaca
     2161 cctgaaaaac ccgctgtcat cagtaccgtt tcgctagttt tgcagcagca gatcatagaa
     2221 aaagacattc cattgctgaa tcaattgcta gatcagccca tacaacctgt aattatcaaa
     2281 agctatcgcc actatattga tttgcagcgt tttaaaggca cacttgtgga accgccagaa
     2341 caaaaacaat atgctttgta tcaaatggcg gtcttagttt ggctgacgca aacgaagact
     2401 ggtgatttgg atgaattaca tttaacaaat ttgaaccatt cattctttgc agatatcgcg
     2461 cacagaggta ttggattttt agcaagaaat cagtcatttt acgaacaaga ctttgttcgt
     2521 ttcttgcaaa agaaaatcag gcaaagtaat tttttgattg tcaatcatgc gtttttagta
     2581 caagaaacac agcgaaaaga accattgctt ccagctagtc catttttgtt gatcgatgaa
     2641 gtacatcatt tgccggaaat cgctgaaaaa gtaaatgaca aacgattaag tattaactat
     2701 ttccataaac aaatccagca attagaagaa ccaaatcagc tgtttgaccg aatacaaagc
     2761 tatttaccga aagaacatga agccttacgc tggttatcga tttatcgaga agaactttct
     2821 gcgctatgcg aagcacagat ttgtatggca gaaggattaa tgagtttagt gagcggcaag
     2881 caacctaacg gacagtatcc agaagaagtc atgatgacaa aagagatgtt ggatcagctg
     2941 ccactggaat ctgggacagc cattgatcac ctgcagcttt tttatcagga gatccagcag
     3001 ctacagtcac aaatgcgtct gacttttgag attatccaag aaagctggac aaaccagatg
     3061 cggatcgatt tagcaagttt atatagccta tttgatcaat tagacaagca atacttgctg
     3121 atggatcaat ggctggaaga ctggcgagat catttgatcc attggttcgt tcctgggaac
     3181 acagcgcagc agatgatttt tcatctccat gattttcaag cggctatgtt gccgtcgact
     3241 gtttggtatc ctcgatatga tcgtattctt tatattggag gaacgctgaa gatcggccct
     3301 aatcgacact atctctctaa gaggcttggc attgaagcag cacctcttaa agtgattgcc
     3361 tctccttatg attatgagaa acaggcaagg atctatgtgc caaaagaggc acctgccgtc
     3421 catcaagtca gcaatcaagc atatatcgat tatttagaga cagcacttac aggtctgatc
     3481 gaacaagaag atcggccaat acttgtgtta ttcacttctc atgatgtttt gcagaaggtc
     3541 tatcagaaaa tgcatctgtc tatgctagaa aaaggacgag aaatactagc tcaagggttt
     3601 ggtggaagcc gggagaagct tttgaaacgc ttcattttgt cagaaaaaag cgtattgttt
     3661 ggcgctgaca gcttttggga aggtgtagat ttgcctggag atgctttgca gatcgttgta
     3721 gtcacacgtt tacccttcga taatccgctt cgcccgatgg tcaaagctag gaacgcttat
     3781 ttagaatcag aggggatcaa tcctttttac caggaatccg tacctaaagc agctttgaag
     3841 ttacgccaag cattaggtcg cttgataagg acggagacag atcgtggcgt attgcttatt
     3901 ttggatcgaa gactagtcac tgcaaaatat ggcgcacgga tcatccatgc attaccgaaa
     3961 aaagtaccga tcaaagagct agctatggag gaaatctatc aggatatcca tgaattctta
     4021 aagaaaaatg aagaaggaag ttagaggagc tgtctttttt cataattaaa atctgctata
     4081 atgaaaagga atgaagataa aggagcgacg acccttgaag aaaaaacaga caaaggaaaa
     4141 aacgattaat cgcgtgttac ttggcttgat cgtattttta ttagcggtca tcgtgatcag
     4201 ctcgattttt tacttgcgtt ccagtcgtcc ggcggtgcag gcgaagaaag aagcagtaga
     4261 aatcgcaaag aaatatgcaa atcttgagga aaccgatcat ttttattggt ttactcgaaa
     4321 gaagacatac ttttcactga caggtacaaa tgataaagga aaagaaatcg ccgttatcat
     4381 tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca aagggactga ctgaaaacga
     4441 agcaaaacag acaattgctg cggcccatcc tgaaatccaa atcgaaaaag ctgctttagg
     4501 gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg ggaaactgaa
     4561 ttattacctg attggctttg aatctggaga tgaagtcaag acaatcaagg gaatttaaaa
     4621 ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa aacgattaga accgtctgtc
     4681 actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga tgtgctgagt
     4741 ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc agcaatcgaa
     4801 gcaatccgca atgggaaagc tagctattat acaccaacag caggaatccc agagcttcga
     4861 caggcaatcg ttgattatct gaagaaaaac tatggacttg tctatgaacc ttcacaaaca
     4921 gtcgtaacag acggagcgaa atttgctttg tatacccttt tccaaacgat ccttgacccg
     4981 caagatgaag tcatcattcc tgtgccttac tgggtcagtt acggtgagca agtaaaactg
     5041 gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa agttactgtt
     5101 gaacagctag aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa ttctccatcg
     5161 aatccgactg gaatgattta tagcaagaat gaactgcagg caatcggtga atgggcagta
     5221 aaaaatgata ttttgatagt tgctgatgat atttatggcc gtttagttta taatggcaat
     5281 gagtttactc caattgcaac gatttcagaa gcaatcaaaa atcagaccat catcatcaac
     5341 ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg gttatgcggt gggaaatcct
     5401 gtgatcatca atggaatgat cgctgttgct tcccaatcaa ctagcaatcc gacagcagtc
     5461 agtcaatatg cagctgttga agcattgaaa ggtgagcaag atacagtaga agaaatgcgg
     5521 aacgcctttg aagaacgcct gaatacgtta tatccgcttg tggcagaact gccgggtgtt
     5581 tctcttgaaa agccgcaagg agctttctat ctttttccta atgtaaaaga aacgctacgc
     5641 atgtgtaagt atgaaaatgt cacacaatgg gtagaagatc ttttagaaga aacaggggta
     5701 gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg tccgcatgag ttatgccact
     5761 gatcgcgcta cgttagaaga agcggtacgc agaatcaagc aatttattga gagtaaaagt
     5821 caaaattaat cctgaagaaa ttcaaggaat ataaaaggag agtcatacgt ggaacaaatt
     5881 caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc ttgggtcgca
     5941 aataagcgat caagcggaaa aatcgcattt ttacaattaa gagacggaac agcctttttc
     6001 caaggtgtag ttgtaaaaag cgaagtgtcg gaagaagttt tccaaacagc taaaaatcta
     6061 caccaagaaa catccgtgtt agtaactgga gaaatccgtg aagacagtcg ttcaaaattt
     6121 ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg agagccacga ttatccgatc
     6181 acaccaaaag aacacggaac agatttcttg atggatcatc gtcatttatg gctgcgttct
     6241 tcaagacaac atgcgattat gcagatcaga aatgaaatca ttcgtgcaac gtatgaattc
     6301 tttaatcaac atcattttgt caaaatcgat ccgccgatct tgactggttc tgcaccagaa
     6361 ggcactacgg atctattcga aacaaattac tttgatcaaa aagcttattt atctcaaagt
     6421 ggccaattat acatggaagc tgctgctatg gctttcggaa aagtcttttc attcggtccg
     6481 actttccgtg ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg gatgatcgaa
     6541 ccagaaatgg ctttcactca tcaagaagaa agtttagaag tacaggaaca atatgtagca
     6601 ttcttagtac aaaatgtttt agatcattgc gaatatgcat tagatgtact aggacgagac
     6661 aaagaagtct taaaaactta tacaaaattg ccattccctc gtattactta cgatgatgca
     6721 atcgaattac tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga ctttggttca
     6781 cctcatgaaa cgttcattgc gaattcattc gaacagcctg tctttatcct aaactatcca
     6841 aaagccatca agccattcta catgaaacca catccaacaa gagaagacgt ggtaatttgt
     6901 gcagatatga tcgcaccaga aggttacggt gaaattattg gcggaagcga acgggcaaca
     6961 gactatgatt tcttgctaga acaaatccgt aatcacggtt tggatgaaaa agaatattca
     7021 tggtatcttg atctacgtaa atacggttct gttccgcact ctggttttgg tctaggcctt
     7081 gaacgaacag tcacttggat tgctggtatc gaccatgtac gtgaagctat tccattccca
     7141 cgtttgttga accgaattta tccataagag gtgatgaaaa agctgttctg catcaagtaa
     7201 ttagaacagc caagcaaaaa cagctgttca tgtttttgaa ttggcgggac ttattaccgc
     7261 agatgtaagc ttttgaatac cgtttagtgg agaggttgtg aaaaagctgt ttagtttcga
     7321 gtattaagaa aaattttgtg gaaatggctt tccacatttt tgcataaatt tggcttaata
     7381 ccgagaaact ggcttttgaa caccgtttaa ggaagaggtt gtgaaatcag tgccttgacc
     7441 tgagtattag agaaacaaaa cggaaaaata gcttctcata ttttaccgtt tttttagcta
     7501 atatcgaggg tcagctgatt gaacaccgtt tatatcaaga ggttgtgcgc ctgccgttca
     7561 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7621 cttgtgaccg aggagttggc cggagagcaa cgtttattta aaaaaaagaa ctgcgaactc
     7681 tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag
     7741 caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat
     7801 atacaaagaa ggttattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa
     7861 agcagcatct agtacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt
     7921 attttcagca tgcaatgatc catatgtaag tttaattcat ccttgaaatc atcggaccag
     7981 ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa aagaacattt
     8041 actggaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat
     8101 tgacggaagc ttaggtgtga tcgatttctc tggaccaaac aacgaaggaa tcaaggggaa
     8161 tccggcacgt taagaaaaaa agtataaaaa actagacact tgatgagcgt tcattcaatt
     8221 gattgaaggg actaggctgg tgtcattttt tatacatata ttttctcttt cgtcttgata
     8281 gggcttatgg gataatataa ggaagaaaat catcgtttta ttcaaaaagg agtttttgac
     8341 aaatgaaaaa acgtatatta tttatcggtg acagcattac agacgcagga agagaccgag
     8401 cagaagcaac tgatctaggt aaaggttatc cacttttgat tgccggaaaa ctaatctccc
     8461 aatatccaac gatgaaattt gaatgcatga accgtgggat aggaggagac aatattgcag
     8521 atctgcaagt acgctggcaa gaagattgtt tagctttaca acctgactat gtatcgattt
     8581 tgatcggaat caatgatacc tggcataata ctagcgatca ggctatattc ggaactgaga
     8641 aatctgcgca gcaatttgaa tccgtttatc gaaaattact aaatgaactt caaacagcgg
     8701 aaatctgcaa catcatcttg atggagccgt ttgtacttcc tataaccgac gaccgtcatg
     8761 gctggcgtaa agacttagac cctaaaatcc aaattgttcg taaacttgct aaggaatatg
     8821 gcgcattgct ggttccttta gatggcatat tcaatgcttt agggattgct tatggttttt
     8881 cgaaatatac aagagaagat ggggtccacc caacgattgg cggacatgag atcattgcac
     8941 aaagctggat agatgcaatt gccgcagtcg atttttttag tggagataaa aatgagcgaa
     9001 agaaaagtga gaaatgatgg aaatacggtg ggcagtaaaa gaagatttac ctcaaatcaa
     9061 tcagctgatt actgatcatc cagaatcatt cgtaaaagca aaaacggcag ctgacctaaa
     9121 tgataagaat aaactattgc tcgttgctgt aacaaaagaa aaacggatta tcggatttgc
     9181 gcaagcggac gtgcaacagt cagaagaacg ggtggatcag cgaccatcag gaaaactaat
     9241 ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc caaaaaaaat ttgcagaata
     9301 cttgatgctg gaagctaaaa aaagaagaat aaaatcaatt tttgcccagt tttaatcaaa
     9361 aacagtaata gaaggagaaa tacaatgagt tgtttaggaa atgtcatctg gtttatattc
     9421 ggcggactaa tcggtggtct tagctggtta gcagcgggga tactttggtg tatcacgatc
     9481 ataggtatcc caatcggtat gcaatgtttt aagatcgcct ccctttcttt tgcgcctttt
     9541 ggcaaagaag taatttatca ccagtctggc atgtcattga tcgttaatat catctggctg
     9601 atcgtttcag gcctaccaat tgctctcgca catgtcgtca gcggcatcct tctatgtatc
     9661 acgatcatag ggataccttt tgccaagcaa tctttcaaac ttgctcgatt ggcgttgatg
     9721 ccattcggtg cagaaattgt ataaaaagag aataataaag gttagaaaac gcatagagtc
     9781 aggtgggata catgtatttc acctgactct atgtgttttc ctgtataagt tctcccatgt
     9841 gacttttacg tcttttattt atatgggatt actgtttatg gaaagttgaa ataattttca
     9901 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9961 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
    10021 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
    10081 gttggataag agcaggggca atgatcgaag agaaaaagaa agaataagcc aaaacatctg
    10141 tatggaagaa
//
LOCUS       DACBZN010000098         9555 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.098, whole genome shotgun sequence.
ACCESSION   DACBZN010000098 DACBZN010000000
VERSION     DACBZN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.098"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..344
                     /locus_tag="IVD64_002281"
     CDS             <1..344
                     /locus_tag="IVD64_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149514.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="IVD64_002282"
     CDS             425..1045
                     /locus_tag="IVD64_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149515.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1746..2030
                     /locus_tag="IVD64_002283"
     CDS             1746..2030
                     /locus_tag="IVD64_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6149516.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2033..2950
                     /locus_tag="IVD64_002284"
     CDS             2033..2950
                     /locus_tag="IVD64_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6149517.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2943..3725
                     /locus_tag="IVD64_002285"
     CDS             2943..3725
                     /locus_tag="IVD64_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6149518.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3726..4406
                     /locus_tag="IVD64_002286"
     CDS             3726..4406
                     /locus_tag="IVD64_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149519.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4399..5295
                     /locus_tag="IVD64_002287"
     CDS             4399..5295
                     /locus_tag="IVD64_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6149520.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5490..7139
                     /locus_tag="IVD64_002288"
     CDS             5490..7139
                     /locus_tag="IVD64_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6149521.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7733..8668
                     /locus_tag="IVD64_002289"
     CDS             7733..8668
                     /locus_tag="IVD64_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149522.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8661..9431
                     /locus_tag="IVD64_002290"
     CDS             8661..9431
                     /locus_tag="IVD64_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6149523.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3056 a         1584 c         2040 g         2875 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtcttaag aaagcccgta
     1261 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
     1321 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
     1381 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
     1441 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
     1501 attatttatc cgcttctgag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
     1561 tttacaaaaa ttgcttctca tgtttgataa atattgtgac ttgtgaccga ggagttggca
     1621 ggagaacacc gtttattcgg aagaagggat tgtgaaaagt cttttgtcat aacctcttat
     1681 ttggtagcta attgtaaatc ccaaacggca tgtattattt ttcataagtc cgtttttaag
     1741 gattaatgtt ttcttttgct ttttatatgg tctggcttca gcgagaagtt ccaacgcata
     1801 tacaaggacg tctgatgtca acgataacaa cgattaccat ggtggccagt ccacttggat
     1861 tagcactgtt tggtgcgctc tttgattggc agacaactca accacgtctg cgagacagtc
     1921 tgatttttct tgaaattcaa ttggaaaatt tacactttct ttatacattt ctggctgctt
     1981 tctttatacc cgtcaactat acttcactta tcaataagaa agaaggataa gtatggattt
     2041 tggaaatgaa acggtcttga aagcaactgg tataaccaaa aaatacggtg ctgccaaagc
     2101 attagataaa gtatcgatcg aaatcaaacg tggaatgatc tatggtttga ttggggagaa
     2161 tggagcgggt aagtctacct ttatgagaac tattatgggt ttgatctcga ttgatgaagg
     2221 ttcgatcgaa ttatttggaa cgacggattt gcaggcagca agacgacgta tgggtcaatc
     2281 gattgaaacc cctgctcttt atccagaatt aactgcaaga gataacctac gtatccaagc
     2341 agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg ctgaaaatga tgcgattgga
     2401 aaatacaggg aaaaagaaag caaagaattt ctctttaggt atgcgccagc gtttagctat
     2461 tgcaaatgca ctgatcacga atccagaatt tttgattttg gatgaaccga cgaatgggat
     2521 ggatccagca gggatggcag aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg
     2581 catcaccgtc ttgctttcta gccacttgct agatgaattg tcacagatcg ccactcacta
     2641 cggtattttg catgaaggac acttgatcaa agaattatcg aaagaagaat tggcacaaga
     2701 atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa caagcagtaa cggttctcga
     2761 ctctcttggt tatagggatt attttgtcca atctagccgt gttatccagc tttttgaagg
     2821 aattgaccaa gtagcagcca tcaatcaagc attggtagag gcaaaagttc ctgtggatgg
     2881 tatccaccta gttgggcaaa aactggaaga ttatttctta caattaactg gaggcaatcc
     2941 acatgtttaa tctattaact gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg
     3001 tctcactagg actgatggtc gtacttccgc ttttttattc ttggaacgaa tggtatatgc
     3061 atacaaaata cggcaatgaa ttaaaccaag caacggatac agtcatcaac ggtgcaacag
     3121 ggattttaat ggtggaaaaa atggctgcat ggattcttct ggctttcgca gcatttgcgt
     3181 gcttttatat tggggaagaa tttcaaaacg gtacgattcg caatacgtta tctttaggac
     3241 ggaatcgaat gacttattat gtgtcgaaac ttctcgttac gcttttgatc acaacacttg
     3301 gcgtggtttt gattacgggg ctagccatga ttgcttacac acttgctttc ggatttggtg
     3361 aagttgaggg aatcaaaaac tatggaaatt atgttctaaa agtatttcct gtattgcttc
     3421 tgctgatatt agcgacatta tctgttccag tggctttgac attcatcact agaagtacca
     3481 gtgtcagttt gcttctttct tttctttata tcatgggaac ggccttcgtt cccggtgttt
     3541 tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt tacggaaacg tggctcatgt
     3601 atacggactt tgcgcaacaa gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc
     3661 tagtaacaat cgtggtatca tcagctctag gaatgtttat tttccaaaaa tctgacatta
     3721 agtaggtgtt cctgctggaa actattttga ttatcgaaga cgatgaagct gttcattctt
     3781 tactggagga ggttcttgaa cagagataca aacttcttga tgcttattca ggtacggaag
     3841 gaaaattatt gttaagcaca tatcctgtcg atttgatcct tctagatctc atgcttccag
     3901 ggctttcagg tgaagcattg ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg
     3961 ttctttcagc aaaaagcgat caaagagata aagtttcctt gttggcagca ggagcggatg
     4021 actatgtgac gaaaccattt gatatcgaag aactgttgtt acgaatcggc atccaacttc
     4081 gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg catctattac aaggaaatct
     4141 tactggacaa agagtcgagg gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa
     4201 gagaattcga tctgctgaag ctttttttag aacatccgaa aaaagtattt tcgcgagcaa
     4261 atctttatga aacagtttgg caagaaccat attttgatag tgagaaaaca gtgaatatgc
     4321 atatttccaa tcttcgaggg aaactagcaa aggcacatgc tgtgtatatt catacggtgt
     4381 ggggagtggg gtttagattt gattaagtgg ctgttgcttc tcgttgttat ctttatggga
     4441 agttttattt tcttcctgca aagggaaatc aagagattaa ctaagaaaat cgccactctt
     4501 tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg
     4561 gacctagtag atgcactgaa tacatgggtg gatgaaaatg aacaaaaagg acaagcattt
     4621 caagaaatgg aagaaaacat cagactatcg atcgtcggcc tttctcatga tctcagaact
     4681 cctttgactg ctattaacgg ttatgtacaa ttactggaac aggtagagga tacggagaaa
     4741 cgggagaagt atttaacgat catcaaacaa tctgttgcaa gattactcag tatgacggat
     4801 cagttttatg atcttgctcg aatagagacg aagcaaaaag aaatggatct ccatacgctt
     4861 tctttttcta aagaggtgga agaagtattt ttctcttttt atgaacaatt ttctgagaaa
     4921 aatatcgagg tagcgttcgc tgaacactcg ataaatacaa ctgtattagc agatccgctc
     4981 atgttgctgc gagtgctgca aaatatcata cagaatttat tgcgttatgc agatggacaa
     5041 gcaaaggtga cattttcaaa agaggattca ttcattcgat taacagtggc caatcctatc
     5101 aaaccaggag cgcaaacctc tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc
     5161 agccgcacaa atacggaatc tagcggaatc gggctttacc tgtcaaaaaa attggtggaa
     5221 ggaatgggag gagagatgac cgctagatta gacgggggca tcttctccat ctccgtcaag
     5281 ttgcggaggg tttaattggc tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg
     5341 ttttaaagac atcttctttt ttggaaggtg tttttatttt ggtgtgattg atacgaatta
     5401 ggtaacgaag acagttattt ttgtaccccc ttttcaaata gcgcaacagt gctatactaa
     5461 aactaacaaa tcaaggaacg gagagggata tgggccaaag agaatttatc gttttaatta
     5521 ttattgcagt tgttatttta ttagttgttt tagacatttt ttcacgtttg aaagtaaagg
     5581 aaacagttcg tagtaattgg ggaaaaattc cttatcaacc gagatttgat aaagaagaaa
     5641 gtttgaagga tgcgtggctc acggagaaaa aattccgttc atgggatagt gaaatcgacg
     5701 atcttacttg gtatgacttg gatatgttcg aagtatttga agggatcaac agtacctatt
     5761 ctagtgtagg ttcagaagcg ttgtatcagc gtttacgtag ctttgatttt ggagaagatt
     5821 atcagttaga aaaactaatt gctttttatc aggaaaatcc tcaactgaga gaaagaatcc
     5881 aatatcaatt cgctcgttta ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg
     5941 atggaaaaag taaaaagatt ggacgcgtag gtttatttat ttttcttggc ttgcttccgc
     6001 tggtgggtgt aggtttgctt cttttagggc aagtactagg aatctatcta gcaatcggtg
     6061 gcttagtatt caatacggtc tattatctag tgaagaaaca aacactagaa acagagctga
     6121 atagcatgag ttatttggtt tcaacgattt cagccgccaa tcagttagca aaaatgacga
     6181 caccacttca aaaagagctg aaacagcaaa ctaaaccatt aagagacatc cctaaattag
     6241 gtttttcatt tcgaatgaag agcgggagtg aagcagaatt gatttttgaa tatgtcaaca
     6301 tcatgttcat gctgccattt atctcctatc atttcgttct cactaaatta gaaaagcatt
     6361 caactgaagc catcaagctg tgggaattgc tgggagaatt ggaagttgca gcagccgttt
     6421 tgaattatcg cacctatatg ccgatcactt gccgaccaga gtttactgag ggcggtgtga
     6481 cagcgaaaga tatctatcat ccattgttaa aagaagcggt agttaatcca gtcaactggc
     6541 agaagaatac attagtcacg ggttccaatg catcaggaaa atctacgtat gtcaaaagta
     6601 tagccatcag ctgtattcta gcacagacga tccaaacagc gattgcagaa cagtttaccc
     6661 tgcaacctgg acatgtgttg acttccatgg caatcgaaga tgatttattt gaaggcgaca
     6721 gttattttgt ttcagaaatc aaatcgatca aacgtttgct ggaccaagtg gcttccaaag
     6781 aacgctgcta ctgcttcgta gatgagattt taaaaggaac aaatacgatc gaacggattg
     6841 cttcttccgc ttcagtcgtt cggtggcttg cggagtatcc atctttagca tttgtcgcca
     6901 cacatgatat cgaattgaca gagattttaa agaattattg tgagaatgtc cactttgaag
     6961 aacaagtaac agaaggaaaa ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa
     7021 ggacccgaaa tgcgattgcc ttgctgaaag ttttgaatta tcctaaatcc atagttgaac
     7081 aggcccaaaa agaaagtttg ttgttcgatg aacagcgtca gtggtatccg tttgattaac
     7141 tttatcaaac gaagcatgat gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg
     7201 atgccaaaaa agtggcagaa ttcaatggga acgagtctgc tgtccagatg atcttggaag
     7261 atttagcaaa agacgataca aaataaacca cgctgtcttc cttctagcag cttttatcga
     7321 aaaaacttag catcagcgac gagacgcacg ttccatggca aagttctccg taaaacaaac
     7381 agcgaaaaca gaaattgaaa ataactattt ccattttatt tatttcggtt aaaataaaag
     7441 aaaaaagctt tcgataaaag gacggaggaa agcttaacat gaataaaaaa gacaagaacc
     7501 tcagttataa ggatcttcta tatgtcatcg tagtagttgc tgtcggtact tttatgattt
     7561 taaaagttcc agagttgttt agatagttat tctatcgaag atgatcatga actagaaaac
     7621 acatctcctc tttgttagca ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg
     7681 ctgtattcca tgatacaatt aagaatcatg tctataagga gataggaaat aaatggcaaa
     7741 tgcgcttgaa ataaataatt tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg
     7801 aatcgatctt tcggtgaaag aaggagattt ctatgcgcta ttagggccaa atggagctgg
     7861 gaagtctaca acgatcggga ttattacttc tttagtcaat aaaacatctg gaaaagtgaa
     7921 tgtcttcggt tatgatctgg acaaggatct agttcgagca aagcaacaga ttggattggt
     7981 tccacaagag ttcaatttta acccttttga aacggtccag caaatcgtcg tgaatcaagc
     8041 aggctactac ggtgtctctc gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca
     8101 gtccaatctt tgggaaaaga gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg
     8161 aagactgatg attgctaggg cattgatgca tgaaccacgt ttgctgatct tggatgaacc
     8221 aacagcagga gttgacatcg agttgagacg cgagatgtgg gagtttctac gagaattgaa
     8281 cgaaaatggt acaacgatca ttttgacgac gcattatcta gaagaagctg aaatgctttg
     8341 tcgcaacatc ggaatcatcc aatcgggtga actgatcgag aatacaagta tgaaagagct
     8401 tctgtcaaaa ctgcaatttg aaacatttat ctttgattta gcgccatatg ataccaaacc
     8461 tgtgattgaa ggatatgctt gtacatttga agatgagcag acaatcgctg tcgaagttga
     8521 acgaaatcaa ggggtgaacg gaatcttcga tcagcttaca agacaaggaa tcaaagttct
     8581 ttctatgagg aataagtcga atcgtttaga agaactgttc ttgaaaatca cagaagaaaa
     8641 taatcatacg gaggagaaaa atgtttaatc tttattttac tgcattaaaa agtttagcta
     8701 tcaaggaaac gaaccgctat ttgcgtatat gggtccaaac actggttccg ccagtgatca
     8761 cgacatcctt gtattttgtt attttcggaa aaatgatcgg tgggcggatc ggtgacatgg
     8821 gtggtttttc atatatggaa ttcatcgttc ctggtttgat tatgatgtct gctatcacta
     8881 gttcgtatgc caatgtttct tcgtccttct tctctcaaaa atttcagaaa aatatcgaag
     8941 aaatcttggt agcacctgtt cctacgcatg tgattatttg gggctttgtg attggtggtg
     9001 tgggtcgcag tattctcgtc ggctctctcg taacgatcat ttccttgttc tttgtaccac
     9061 ttcatgtgta ttcatggttc atggtcatca ttacattatt gatgacggca atcttgtttt
     9121 cattagcagg actgctcaac gggatttttg ctcagtctta tgatgatgta tcgatcgtgc
     9181 ctacatttgt cttgcagccg ttaacttatc taggtggcgt tttctatgcc atctctatgt
     9241 taccgccctt ttggcaagct gtttcaaaag tcaatccgat cgtttatatg atctccggtt
     9301 tccgttacgg attccttgga acgatcgatg tgccagtagc aacatcgatg atcgtacttg
     9361 tgttattcat cgttgttttg tacgccgtta cttggtattt gatcaataaa ggaagaggat
     9421 tgagaagtta gaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
     9481 cgggaaaata gttcctcata ttttgttgtt ttttgagcga ataccgaggg tcagctgatt
     9541 gaacaccgtt tagtg
//
LOCUS       DACBZN010000099         9377 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.099, whole genome shotgun sequence.
ACCESSION   DACBZN010000099 DACBZN010000000
VERSION     DACBZN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.099"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            69..139
                     /locus_tag="IVD64_002291"
     tRNA            69..139
                     /locus_tag="IVD64_002291"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101..103,aa:Gly,seq:tcc)
     gene            153..226
                     /locus_tag="IVD64_002292"
     tRNA            153..226
                     /locus_tag="IVD64_002292"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Ile,seq:gat)
     gene            251..338
                     /locus_tag="IVD64_002293"
     tRNA            251..338
                     /locus_tag="IVD64_002293"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:285..287,aa:Ser,seq:gct)
     gene            360..431
                     /locus_tag="IVD64_002294"
     tRNA            360..431
                     /locus_tag="IVD64_002294"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Glu,seq:ttc)
     gene            454..543
                     /locus_tag="IVD64_002295"
     tRNA            454..543
                     /locus_tag="IVD64_002295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Ser,seq:tga)
     gene            556..629
                     /locus_tag="IVD64_002296"
     tRNA            556..629
                     /locus_tag="IVD64_002296"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:590..592,aa:Met,seq:cat)
     gene            648..720
                     /locus_tag="IVD64_002297"
     tRNA            648..720
                     /locus_tag="IVD64_002297"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:681..683,aa:Asp,seq:gtc)
     gene            724..796
                     /locus_tag="IVD64_002298"
     tRNA            724..796
                     /locus_tag="IVD64_002298"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:757..759,aa:Phe,seq:gaa)
     gene            801..871
                     /locus_tag="IVD64_002299"
     tRNA            801..871
                     /locus_tag="IVD64_002299"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:833..835,aa:Gly,seq:tcc)
     gene            884..957
                     /locus_tag="IVD64_002300"
     tRNA            884..957
                     /locus_tag="IVD64_002300"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:918..920,aa:Ile,seq:gat)
     gene            969..1040
                     /locus_tag="IVD64_002301"
     tRNA            969..1040
                     /locus_tag="IVD64_002301"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1002..1004,aa:Glu,seq:ttc)
     gene            1306..2499
                     /locus_tag="IVD64_002302"
     CDS             1306..2499
                     /locus_tag="IVD64_002302"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP6149524.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2629..4101
                     /locus_tag="IVD64_002303"
     CDS             2629..4101
                     /locus_tag="IVD64_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6149525.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4184..4972
                     /locus_tag="IVD64_002304"
     CDS             4184..4972
                     /locus_tag="IVD64_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP6149526.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5039..5707)
                     /locus_tag="IVD64_002305"
     CDS             complement(5039..5707)
                     /locus_tag="IVD64_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6149527.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5822..7534
                     /locus_tag="IVD64_002306"
                     /pseudo
     CDS             5822..7534
                     /locus_tag="IVD64_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            7537..9309
                     /locus_tag="IVD64_002307"
     CDS             7537..9309
                     /locus_tag="IVD64_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149528.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2768 a         1641 c         2117 g         2851 t
ORIGIN      
        1 gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat tcccgtcgga
       61 accgccatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt tgtcgcgagt
      121 tcgattctcg tcacccgctt ttagtttttt atgggcctat agctcagctg gttagagcgc
      181 acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattag tacaaaatct
      241 tattggtttt ggggaagtac tcaagtggct gaagaggcgc ccctgctaag ggtgtaggtc
      301 gggaaaccgg cgcgagggtt caaatccctc cttctccgtt tcaatatttt gttttacctg
      361 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      421 cgtacgggtc attaatttaa tatagtttta catggaggat tacccaagtc cggctgaagg
      481 gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa gggttcgaat cccttatcct
      541 cctttttcat attatcgcgg gatggagcag tctggtagct cgtcgggctc ataacccgaa
      601 ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa aaacattggt tccgtggtgt
      661 aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc gtcggaaccg
      721 tttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt cggcagttcg
      781 attctgtccc gcgccattat gcgggtgtag tttagtggta aaaccacagc cttccaagct
      841 gttgtcgcga gttcgattct cgtcacccgc ttttagtttt tatgggccta tagctcagct
      901 ggttagagcg cacgcctgat aagcgtgagg tcgatggttc gagtccattt aggcccattt
      961 attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg
     1021 ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt tttatctgaa
     1081 agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct aagctttttt
     1141 ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa aaaatacgag
     1201 cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag gatccgcagg
     1261 acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt agaaagacac
     1321 ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga ccaaatcagt
     1381 gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg
     1441 tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc atatgtagat
     1501 attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc aaaattcgga
     1561 tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc tgatattgct
     1621 caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt attagatgag
     1681 attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac accagaattg
     1741 atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa
     1801 tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt tgaatatgat
     1861 gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca tgatgatgca
     1921 gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga agttattcca
     1981 gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg atttgtcatt
     2041 ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga tacgtatggc
     2101 ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa agttgaccgt
     2161 tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg gcttgctcgc
     2221 aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc
     2281 aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt gagagaaaac
     2341 ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa
     2401 caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg ggaacatacg
     2461 gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag ctggaacgat
     2521 cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa aaagatagta
     2581 attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat gaaaaaagaa
     2641 acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac ggctattgaa
     2701 ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg aatggaaatc
     2761 atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc gatttacgga
     2821 aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt attgtttatt
     2881 atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc tgctcgtgcg
     2941 attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat cggcgatctt
     3001 tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg ggggattgcc
     3061 agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg gcactggatc
     3121 ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct atatttaaat
     3181 gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt tagtttgatg
     3241 gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg aatgagtccg
     3301 cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt ttttgccgaa
     3361 aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact atttgttgtg
     3421 gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt ttatatccct
     3481 atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt attagcacca
     3541 ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac tcaaatgaaa
     3601 actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc ttggttgtat
     3661 cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt catcggtata
     3721 ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt atctcgtgac
     3781 cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca aacagtcatg
     3841 gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc agcagcaaat
     3901 ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc taaatatatt
     3961 gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca ttccgtttat
     4021 ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa
     4081 agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat cattcaaatt
     4141 taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat ttattcaagc
     4201 aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac taccgcaaac
     4261 aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac ctgttttata
     4321 tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt cgattgaacg
     4381 ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg gctggtacac
     4441 agatacagtt tatgacatga agtattggac attcattgca gaagagctac ctgagatttg
     4501 tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg ctggattatc
     4561 gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt
     4621 aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt tgatggttcg
     4681 taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag gttcaaaaaa
     4741 tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc caagatttta
     4801 tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg tatctgctat
     4861 gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata actggatctt
     4921 ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag
     4981 tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag gagctttttt
     5041 actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag gtttgtgcac
     5101 tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt tctttaaatc
     5161 cctctaattg attttcaact acataattac gcataaatag aactgtttct ttcaaaaatt
     5221 catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc attaactgtc
     5281 caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact gaaaatcctt
     5341 cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga aaagcgggca
     5401 cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca tagtaatttt
     5461 ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc gctaattctt
     5521 cttccattgt ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa taccaatata
     5581 aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc tgcgaaagag
     5641 cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga gaaagcttta
     5701 tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa attgttagtc
     5761 ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt gtaatcaacg
     5821 aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta tttccttgct
     5881 gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac agcaagtatt
     5941 ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt acctgatcag
     6001 tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg caaaagtagc
     6061 tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga cattttcatt
     6121 tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac
     6181 gcaaatccaa aattaagtga tgatttcttt gcaatcactt tttcgaattc cattcttatt
     6241 tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc
     6301 gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg gcaagcactt
     6361 catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg
     6421 gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt
     6481 aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg tgatgatccc
     6541 gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg tgagcgatct
     6601 tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact acttgatgca
     6661 gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa gagcagccgt
     6721 atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt atttagacgt
     6781 tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc gttatcctgg
     6841 tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag aaatggttgg
     6901 tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc ctcgtctatt
     6961 tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa
     7021 gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct caggaacgat
     7081 tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc
     7141 ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg aagcaccaat
     7201 agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg
     7261 cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac tggatgctcg
     7321 ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa cgacgattgt
     7381 catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat tggatgaagg
     7441 cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg tctatcagga
     7501 aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat aaaagcaagt
     7561 aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat ctttatcaca
     7621 gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga agcttttcaa
     7681 gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag caaatttgtt
     7741 tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa ctttatctat
     7801 agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat cggtctattc
     7861 aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg tgaaatttta
     7921 agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc tttgcttcaa
     7981 gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg ccaaaatgta
     8041 gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc tgtcttgatt
     8101 atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa actaaatggt
     8161 tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt acaagaagaa
     8221 acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt caaaggacaa
     8281 gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa tacagcgata
     8341 gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc ggtggcatta
     8401 ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact gatgcagatt
     8461 tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt gaatgaaata
     8521 tttgatgaaa aagatgaagt caaaccagaa aacggtaaag catttgatgg tgttgaaaaa
     8581 ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt cttgaaagat
     8641 gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac aggatcgggg
     8701 aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc
     8761 atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca tgtaggaatc
     8821 gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac attcggcaaa
     8881 ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat tcatgacttc
     8941 atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa cttattcagt
     9001 acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc atctttattg
     9061 attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat ccaaaaagca
     9121 atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct gaaaacaatc
     9181 ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga aggcagtcat
     9241 catgagttat tagcagaaaa tggattttat gcggaacttt atcataatca atttgtattt
     9301 gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt ttatgttgtt
     9361 tgtttttata aggggca
//
LOCUS       DACBZN010000100         9024 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.100, whole genome shotgun sequence.
ACCESSION   DACBZN010000100 DACBZN010000000
VERSION     DACBZN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.100"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            96..743
                     /locus_tag="IVD64_002308"
     CDS             96..743
                     /locus_tag="IVD64_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149529.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(836..3115)
                     /locus_tag="IVD64_002309"
     CDS             complement(836..3115)
                     /locus_tag="IVD64_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6149530.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3393..5216
                     /gene="cadA"
                     /locus_tag="IVD64_002310"
     CDS             3393..5216
                     /gene="cadA"
                     /locus_tag="IVD64_002310"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6149531.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5263..5442
                     /locus_tag="IVD64_002311"
     CDS             5263..5442
                     /locus_tag="IVD64_002311"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP6149532.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5779..7719
                     /gene="thrS"
                     /locus_tag="IVD64_002312"
     CDS             5779..7719
                     /gene="thrS"
                     /locus_tag="IVD64_002312"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP6149533.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7943..8791
                     /locus_tag="IVD64_002313"
     CDS             7943..8791
                     /locus_tag="IVD64_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6149534.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT         2856 a         1596 c         1902 g         2670 t
ORIGIN      
        1 atttattttc ttttctaatt caacctgtgg tatgatgaga gcataagtat catgtgaaat
       61 gatatgaagt atgtggaaga tggacgggga agaatatgga agcaaaaatc actttagaac
      121 cttttgaacg tatcttaagc ggttaccgca aagtagaaga gttagctgtg aacgtaactg
      181 attgttcaaa attagcgcaa aaatacgcaa gattcggcgt tgaaggctat cgattaggaa
      241 actatgtcgg tacagggtat ctgaaccgtt atttagagtg tatggtggac cgcgcaccaa
      301 tgctgattta tagacaaaat tatctgattc cgctgctttt tcgtcgttct gattcagcct
      361 ttcggttatt tgaagaagag tatcggatgg aggctttctt tctactatta gagtggagct
      421 tgaaacatcg tcctgaaaaa attcttatcg aaagaaatga gaagatagac acgaaaaaaa
      481 ataaagtgat cgatagtgca tacctagcat ttcgtgtatc agagattttg gattgtggcg
      541 gctatccaat cagcaatttc caatcaattg accaattcat tgaatggaat cgaatttacc
      601 gattgatcga caatggcggg attggccgtc atagcaaggt atttgaccca gaatatccag
      661 aaaacatgga agaactgaaa atgattattt cgcttgtcaa attaaaatat cctgaaacag
      721 atttggattt atatatagaa taaatgacct taaatgattg aattactttt tctgcaatta
      781 ttattgtaaa taaaaagaca actgctaaca ttaatgatag cagttgtctt tttacttaca
      841 gtactttttt ctcataagat gaaatatcca tattgtttaa caaaggactt tcattattgg
      901 aggtaaaaag ggtcaattca tgcatcgcac gtgtacacat ggtgtaaagg atcaatttgt
      961 cttgctgaga agaaaaatgt tctgcatcta catcccacaa gaaaactcgg tcaaactcca
     1021 gaccttttgc taaaaaggct ggaagtaaaa taatggaacg cttcataaag tcactctcat
     1081 ttaaaatcag ctggacttgt tgtttcaact cgtctggcag ttgagcataa agagcttcgc
     1141 attcttcttg tgttttagct atgattgctg tacgccaata ccgggctttt ccctgagtcg
     1201 ttcgaatggt atctgctaac caattcaatt tgatatcatc tgttgttcct tggatgagca
     1261 aaggaaggtc tccttttctt gctgttgttt ctacttgatg ttctttcaca ataaaatgat
     1321 tagcaaaatc agtgatttct tttgtggagc gatagcttgt cgttaattgg aaacgagtga
     1381 cttcacggtc cacgaaaagg tcttccaatg atccaacgat cgtttcattt ccaaagacat
     1441 tttgatttag atctccacag aaagtcaaat ttgcttttgg gaacaaggct tgcagcaatg
     1501 ccacttgtga tggtgcgaaa tcctgcattt catcaataaa gatatagcga gctttgtgtg
     1561 taaccgtcac tggatatgtt cggcgcatca gtaaaaagaa tagtacagca tcctcttgac
     1621 gcaattctct ttgacgcaag tgtgtacgaa cagttgtcaa atgttcctgc cacctggtct
     1681 cgctgatttt atgtcgtgtt agaagagaaa gtggcacagt ttgtagaaag tggagatatt
     1741 gtttcgttat attgacaaat tgataattca tgatccctcg atggattcga cggaatcttt
     1801 tacgtacgat ttggttggcc agttttttac gtaatgaacg ctccttttgt tctgtatatt
     1861 caagatttgg atctgttgcg tatagttctt ggagctgttc ttccgccaga tctttgaccc
     1921 attgctgacg tgtttcgtct ttttgcagac cgcctaattt tttcaggagc ttcgtttgta
     1981 atagagaaag tctatgatgt aaaggtaatt gttcatttgt ttctttatac cattggcgaa
     2041 tcgattcctt tgataggatc gtacggccat ttattttcat atcacggaac aagggaccgt
     2101 agtttgtgat gccttggata tagcgatcaa tctgatcgac taaggtcaat ccgcttttca
     2161 tcacttgtat cggatctttt tcccctagca aaaaaccact ttcttgttgc tgttcatcta
     2221 gtaaagaaaa ctctggcagc agctgcacca aataattttt gaatgtctgt gtaggcacgc
     2281 cgctttcacc taatgaaggc aatacagtcg agatgtaatc agaaaacaaa tggttgggtg
     2341 aaaatagaag aacgttttca gcctcgagcc acttgcgatt atgatacaac agaaaagcaa
     2401 tacgttgaag caaagcggat gtctttccac ttccggcgat tccttcaatc agcattactt
     2461 tacttgttgt gtcccgaatg attgcatttt gtgctttttg tatcgtagag acgatgtttt
     2521 tcatatggtt gcttgatgat tcatccaata tctctagcaa aaagtcatca ttgattactt
     2581 ccgacgtatc taccatagat aaaatacgtc cttcttggat tttaaattgt cgtttcaact
     2641 gcagatcgac atctattcta tctgtttcag tttcataaaa tgcgggacca atctcacctt
     2701 cataataaag attggcaatt ggtgcgcgcc agtcaataac gattgtttct tctgccagat
     2761 ctcttaaaga agcgatacct agatataatg tttctgtttc acggtcttct ttaaagtcga
     2821 tacgggcaaa ataaggacta tctttcatga cagacaacgt ttgcaggcgt ttttgctgtg
     2881 cttcaagagt atggtatttc aaaagcaatt cctgctcatg ttggcgatat tcaacagcag
     2941 attcataaaa agcttcattg gaaccagctc gaatcttgtg attagctgtg tcttttgtcg
     3001 aatgattcat ttcctgagtc aattcttgtt gttttgtatc cagcatattt tgttcgatct
     3061 ggattagttc gatcgtttca tccacgtgcg tttgttctag ttgtttctca ttcatcgcat
     3121 gtttgctcct tccgccgtaa ataacgaatt aaaagtatag catagttttt cgaaaaaagg
     3181 tactttttaa ctgatgaatc gaaaaagtaa tccttgctga atatttttgt ttgtagaact
     3241 gatgaatttt ggaaacgctg atagtgatag ttgttttcaa ttaaggccta ttgttttaaa
     3301 aagtgctagt caaggactat gcttaagatg aaagaggcag ctggaaattt attactaacc
     3361 tctctaagtc acataaaaga ggagaaaaaa caatgacaca tttcaaaaaa tttttactta
     3421 ctttgcttat tggtgggata gctttattaa gtgaatttgt ttttcaccag ccggcccttg
     3481 catttgcgat tgtcgcattt actggaggga ttctcgcctt cttgatgttt ttggaaatga
     3541 tcaagacgtt acgttcagga agatatggcg tagatgtatt agcgattacc gcaatccttg
     3601 caacattagc cgtcaaagaa tactgggcta gtttgatgat attgatcatg cttacgggcg
     3661 gagatagtct agaggattac gcgaataaac gagcgggtaa agaactgcag tctttgttag
     3721 ataacacccc aagaattgcc catcgattca aagaaggaaa attgcaggat atagtaatcg
     3781 atgaaataaa tattgatgac cagttactag tcaagcctgg cgaacttgta cccgcagatg
     3841 gtcgagtgac caaaggagaa tcttcggtcg atgaatcttc tttgacaggt gagtcccgcc
     3901 cggaaaccaa gaaacctgga gatcagatta tgtcagggtc tttaaatgga gacggatcga
     3961 tccaaatgat cgctgaaaaa cgtgccgcag acagccagta tcaaatgatt attaaactag
     4021 tcgaagaatc aaaagaaaag cctgcccgat ttgtccgttt agctgatcgt tatgcagtcc
     4081 ctttcacgtt gattgcttat ttgatcggtg gtatttcttg gtggttctca aaagaccctt
     4141 tacgatttgc tgaggtactg gtagtggcat caccttgtcc actgattcta gctgcgccca
     4201 ttgctttagt tgcaggaatg agccgttcaa gtaaaaacgg gatcgttgta aaaactggaa
     4261 caacgattga aaagctcgca cgtacaaaaa cgattgcttt tgataaaaca ggaacgctta
     4321 ccaaagggat attggaagta gaggcgattc agccagtcaa tggaataaaa aaagaagagc
     4381 tacttctgta tgcagctagt gcagaacagg aatctagcca tatcttagct cgttcgttag
     4441 tgaacaacgt gaaaaacagc caactgtatc ctgttagtca actgaaagaa gtgaccggtc
     4501 aaggaatcca agcagtagtc aataatcatg aagtaaagat tggacgtgcc tcttttgttt
     4561 cagctgaaga aatagaaacg tccaatacag caatctatgt aacaattgat cgatcttttg
     4621 caggaacgat cctattttcg gatcagattc gcccagaagc agcaaagaca atcaaggagc
     4681 taagaaaact tgatgtgaaa aatttgatga tgatcaccgg ggatggatct gccattgctc
     4741 aaacgattgc agaaaaagtc caattagatt ctgtacatgc ccgtagcttg cctcaagata
     4801 aactgaccat tttagaaagt atccctaaag atcaaagacc tgttgcgatg gtaggagacg
     4861 gcgtgaatga cgcaccagca cttgctgctg cagatatcgg tattgcaatg ggggctcatg
     4921 gttctacagc tgctagtgaa agtgcagatg cggtcatctt gaaagatgat ctcttgcgtg
     4981 tagcaaaagc agtagtgatt tcaagagata cgatgagaat agcgaaacag tctgtactga
     5041 ttggcatctt tatttgtatt tttttgatgt taattgcaag tacgggacta atccccgcat
     5101 tattcggagc tgttttgcaa gaagtcgtgg atacggtctc tattttgagt gccttgagag
     5161 caaaaaaaga tccagccatt tttcctgatt cagtacccaa gacaaacata gtatgataga
     5221 atcgagttga aaaaagaacg atcagaaaga ggagagagca ttatgccatt cgtacatgtc
     5281 gaattagtag aaggtcgcag tccggaacaa ttagaaaata tgatgaaaga tatcacggaa
     5341 gctgttcata aaaacacgca ggcaccaaaa gaacacatcc atgtcatcat caatgagatg
     5401 aaaaaaggaa catacggtgt aaatggggaa tggaaaaaat aagtttagtt tcccacttga
     5461 tttttctatt tattagtggt taaataaaca cataattgat tgatgagaaa gccaactgaa
     5521 tttgagagtc ttcgataaga gaggaaaagc taggctgaaa cttttccagg agaaaaccaa
     5581 aggatgacca ctttcgaaac ccttgttgaa gcaagggcgg tcggaccgtt acaacgcttg
     5641 aggaacgcat ttgcgtttga acattaggtg gaaccacgaa cttttcgtcc tagtatctct
     5701 tttcaagaga tgctaggatt tttttgtttt tcagcttgat gatctgatca atcaaaacat
     5761 ttcaaggagg aaactattat gataaaaatt acttttccag atggtgcagt caaagaattt
     5821 gagtctggaa caacaacatt ggcaattgct gaaagcattt caaaaagctt agcaaaaaaa
     5881 gctttagctg gtaaagtgaa cggaaaactc gttgatttga ctcgtccaat cgaggaagat
     5941 gcaagtattg agatcatcac accagaccat gaagatgcac ttggcttagt ccgtcattca
     6001 gcagctcatt tgatggccca agccatgcgt cgcctctatc caaatatcca ctttggcgtt
     6061 ggaccagcta tcgattcagg gttttactac gatacagata atggacaaaa ccaagtaact
     6121 gcagaagatt tgccggcaat cgaagcagaa atgatgaaaa tcgtcaaaga aaatctgccg
     6181 atcgaacgcc gtgttctttc aaaacaggaa gcattagaaa tttttgcaag tgatccgtac
     6241 aaagtagaac tgattagtga attgccagaa gaagaagtga ttactgctta tcaacaaggc
     6301 gaattcatcg atttgtgtcg tggaccacat gttccatcca ctggccgtat acaagtattt
     6361 aaattattat cagtggcagg tgcttattgg cgtggaaact caaacaatca aatgatgcaa
     6421 cgtgtatacg ggacagcctt ttttgacaag aaagctttga aagaatatat ccgtctccgt
     6481 gaagaagcaa aagaaagaga ccatcgtaaa ctaggaaaag aacttgactt gttcatggtt
     6541 tccccagaag taggttctgg tttgccattc tggcttccaa aaggggcaac aatccgacgc
     6601 acgatcgaac gttatatcgt agacaaagaa gttagtctag gttaccaaca tgtatataca
     6661 ccaataatgg gagatgttga gttgtacaaa acatctggac actgggatca ttatcaagaa
     6721 gacatgttcc cgccaatgga tatgggagat ggcgaaatgc ttgttcttcg cccaatgaac
     6781 tgccctcatc acatgatggt ctacaaaaac acgatccatt cttatcgtga attaccaatt
     6841 cgtatcgctg aattaggaat gatgcatcgc tatgaaaaat caggtgcttt gtcaggttta
     6901 caacgtgtac gagaaatgac gttgaatgat ggtcatacat ttgttcgtcc tgatcaaatc
     6961 aaagatgaat ttaaacgtac gcttgaattg atggtggcag tatatgctga cttcaatatt
     7021 acagattatc gtttccgctt gagctatcgc gatccaaaca atacagataa atattttgat
     7081 gatgacgcaa tgtgggaaaa agcccaagca atgctgaaag ccgctatgga tgaattagaa
     7141 ctagattact ttgaagcaga aggcgaggca gccttttacg gaccaaaact agatgttcaa
     7201 gtcaaaacag ctcttggtac agaagaaaca ttatcaacta tacaattaga cttcttattg
     7261 ccagagcgct ttgatttgac atatgttgga gaagacgggg aaaatacgca tcgtccagta
     7321 gttatccacc gcggaattgt ttctacaatg gaacgttttg tcgcttattt aacagaagta
     7381 tacaaaggcg cttttccaac atggttggca cctatacaag caacaatcat ccctgtttct
     7441 gttgatgcac atggcgatta tgcttatgaa atcaaagaac gtttgcaaat gaaggggttg
     7501 cgtgtagaag ttgatgatcg taatgaaaaa atgggctata agatacgtgc ctctcaaaca
     7561 caaaagatcc cttaccaatt agttgttgga gacaaggaat tggaagatgc aacagtgaac
     7621 gtacgtcgtt atggaagtaa agaaacagct gtagaagatt tgaatatctt tattgatgct
     7681 atggaagcag aagtcaaaaa ttacagcaga gaaaactaat aggtaaattt cctgtaaaaa
     7741 agtagtagat agaaagcaat ccgaacaata gataacaggt attcctcaaa gacacagatg
     7801 ctcaatagaa tctgtgggat tgatgggata agatattctg ttgggcggat tgtttttttg
     7861 ttttttatcc agtatacgac cgtgaataag ggtctgatat ggtatactta catagactat
     7921 cattaaaagg gtggaggaac ttatgagcgt aattagtcgt attgaaagta tgatggaaga
     7981 ttattcttca gcagaaaaaa agttggccaa ctacattatc ggtaatattg agaaggtccc
     8041 tacaatgaca gctaatgaat tagcagaagc ttccgggttg agtgcgccga cagttgtacg
     8101 tttttccaaa aaaatcggtt ttcaaagtct gacggacttc aaaatcacga tttcaacaga
     8161 actacagact ggaatcgatg aaggttttag tgatatagaa gcaaatgagt ccttttactc
     8221 tatcaaaaac aaacttggta acaatgcaca agtagcgata aaagaaacag tagatattct
     8281 agaagaagaa acgatccaaa aagtcgtaga gtgtttggaa tcggcagaga ctgttttttt
     8341 atatggggtt ggcgcctctt ctttagtagt agaagatatt ctgcaaaaat ggtctcgtgt
     8401 cggtaagcca atcatttttg aaaaagatat ccacgtcttg ctcccgcaac ttgtcagcaa
     8461 tgagaaaaag aaagtgcttt ggcttgtttc aaactcaggt agaagtgcag atgtagtcgc
     8521 attagccgag ctggcgaaaa gcatgaatat cgaaatcatt gcgttgacgc aatttggaaa
     8581 taacccgctt agcaagatag ccgatgtact cgtacaaaca tctagaccaa aagaaatcac
     8641 gaatcgaagt gcggcaacta attcactttt ggcccaattt gctacgatcg atatcatttt
     8701 ttatttctat atggctaaaa acgaaaagct tagcgaaaaa gtgaaaaaaa cacgagaagc
     8761 aatccaagaa tattttatgg attcgaaata aaaaccttac tattttttat ataagttttt
     8821 tagaataaca aaacgtagtt ttccacaaat aaatgatcca tcaaagtgtt tcacgactta
     8881 agaaaacata attttcaaaa ataagtttat tttttaatga aaaaaatagt tggatatgta
     8941 actttaagtg attgctgtga aacaaaaagt tgattttatc ttgaaattag gtaaagaatt
     9001 tcttgctccg cccatttttt cttt
//
LOCUS       DACBZN010000101         8969 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.101, whole genome shotgun sequence.
ACCESSION   DACBZN010000101 DACBZN010000000
VERSION     DACBZN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.101"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(57..350)
                     /locus_tag="IVD64_002314"
     CDS             complement(57..350)
                     /locus_tag="IVD64_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP6149535.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IVD64_002315"
     CDS             complement(378..1043)
                     /locus_tag="IVD64_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP6149536.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IVD64_002316"
     CDS             complement(1183..1815)
                     /locus_tag="IVD64_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149537.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IVD64_002317"
     CDS             complement(1822..2889)
                     /locus_tag="IVD64_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6149538.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IVD64_002318"
     CDS             complement(2886..3617)
                     /locus_tag="IVD64_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP6149539.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IVD64_002319"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IVD64_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP6149540.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IVD64_002320"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IVD64_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP6149541.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="IVD64_002321"
     CDS             5780..6949
                     /locus_tag="IVD64_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6149542.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7216..8838)
                     /locus_tag="IVD64_002322"
     CDS             complement(7216..8838)
                     /locus_tag="IVD64_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149543.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2856 a         1701 c         1333 g         3079 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     7321 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
     7381 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
     7441 tacaggtaaa actattgttt tgccttttga agatttaagc gtaaatgtaa tttcatcttt
     7501 atttggagaa aagctactat aaataacttg accatcttct gtaacttgat ggtttttcaa
     7561 aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa attcttcaag
     7621 tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt tcaccatgtt
     7681 aactactgat gaataattta aaattgaaat agctaatatt aataatagta taaaatttct
     7741 tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata ctgaaaaaga
     7801 tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat ttaaaggagt
     7861 attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata ttatacccat
     7921 tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta gtataattaa
     7981 acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta tacttccaat
     8041 caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat aaggtctatt
     8101 cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg aaagaagtaa
     8161 agtaactata gcagctttta ttaatttaat aattaagaag atttctattt tctttttccg
     8221 ataaaattga ataattaaag caattataat cattaccata gtcatagcta cagaaagaac
     8281 atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt ctttttttga
     8341 gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg cgagtaattc
     8401 ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg tatatataat
     8461 agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat aatgacatat
     8521 aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg ctaaattagt
     8581 aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat tatttacttg
     8641 catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag attctaaact
     8701 taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca tgtttttaaa
     8761 tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt ttacagaaaa
     8821 aatttttttc aaattcatat accacctcat gtttttttat attacatata gtatcattta
     8881 tttttttaaa acactagatt agtcactata taaaatgtga ttaattttat tattaataaa
     8941 atatttttta tattacaaaa gtagtgttt
//
LOCUS       DACBZN010000102         8942 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.102, whole genome shotgun sequence.
ACCESSION   DACBZN010000102 DACBZN010000000
VERSION     DACBZN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.102"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            51..260
                     /locus_tag="IVD64_002323"
     CDS             51..260
                     /locus_tag="IVD64_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149544.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IVD64_002324"
                     /pseudo
     CDS             467..667
                     /locus_tag="IVD64_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IVD64_002325"
     CDS             complement(1379..1984)
                     /locus_tag="IVD64_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6149545.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IVD64_002326"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IVD64_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IVD64_002327"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IVD64_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IVD64_002328"
     CDS             3019..3990
                     /locus_tag="IVD64_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6149546.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IVD64_002329"
     CDS             4130..5443
                     /locus_tag="IVD64_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6149547.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IVD64_002330"
     CDS             5486..6352
                     /locus_tag="IVD64_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6149548.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IVD64_002331"
     CDS             6349..7197
                     /locus_tag="IVD64_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6149549.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IVD64_002332"
     CDS             7211..8683
                     /locus_tag="IVD64_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP6149550.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="IVD64_002333"
     CDS             8700..>8942
                     /locus_tag="IVD64_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6149551.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DACBZN010000103         8841 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.103, whole genome shotgun sequence.
ACCESSION   DACBZN010000103 DACBZN010000000
VERSION     DACBZN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.103"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(12..392)
                     /locus_tag="IVD64_002334"
     CDS             complement(12..392)
                     /locus_tag="IVD64_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6149552.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(389..775)
                     /locus_tag="IVD64_002335"
     CDS             complement(389..775)
                     /locus_tag="IVD64_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149553.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(777..1055)
                     /locus_tag="IVD64_002336"
     CDS             complement(777..1055)
                     /locus_tag="IVD64_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149554.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            1103..1831
                     /locus_tag="IVD64_002337"
     CDS             1103..1831
                     /locus_tag="IVD64_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP6149555.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(1977..2453)
                     /locus_tag="IVD64_002338"
     CDS             complement(1977..2453)
                     /locus_tag="IVD64_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP6149556.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(2413..2760)
                     /locus_tag="IVD64_002339"
     CDS             complement(2413..2760)
                     /locus_tag="IVD64_002339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149557.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(2747..2932)
                     /locus_tag="IVD64_002340"
     CDS             complement(2747..2932)
                     /locus_tag="IVD64_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149558.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(2933..3205)
                     /locus_tag="IVD64_002341"
     CDS             complement(2933..3205)
                     /locus_tag="IVD64_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP6149559.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(3202..3405)
                     /locus_tag="IVD64_002342"
     CDS             complement(3202..3405)
                     /locus_tag="IVD64_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149560.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            3530..3742
                     /locus_tag="IVD64_002343"
     CDS             3530..3742
                     /locus_tag="IVD64_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149561.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(3775..3969)
                     /locus_tag="IVD64_002344"
     CDS             complement(3775..3969)
                     /locus_tag="IVD64_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149562.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(3977..4165)
                     /locus_tag="IVD64_002345"
     CDS             complement(3977..4165)
                     /locus_tag="IVD64_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149563.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(4792..5157)
                     /locus_tag="IVD64_002346"
     CDS             complement(4792..5157)
                     /locus_tag="IVD64_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149564.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(5197..5499)
                     /locus_tag="IVD64_002347"
     CDS             complement(5197..5499)
                     /locus_tag="IVD64_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149565.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(5499..5798)
                     /locus_tag="IVD64_002348"
     CDS             complement(5499..5798)
                     /locus_tag="IVD64_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149566.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(5961..6230)
                     /locus_tag="IVD64_002349"
     CDS             complement(5961..6230)
                     /locus_tag="IVD64_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149567.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(6242..6991)
                     /locus_tag="IVD64_002350"
     CDS             complement(6242..6991)
                     /locus_tag="IVD64_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6149568.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(6994..7680)
                     /locus_tag="IVD64_002351"
     CDS             complement(6994..7680)
                     /locus_tag="IVD64_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149569.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(7686..8357)
                     /locus_tag="IVD64_002352"
     CDS             complement(7686..8357)
                     /locus_tag="IVD64_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP6149570.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(8350..8691)
                     /locus_tag="IVD64_002353"
     CDS             complement(8350..8691)
                     /locus_tag="IVD64_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149571.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
BASE COUNT         2430 a         1788 c         1375 g         3248 t
ORIGIN      
        1 ataggggggc ttcaattgaa atacgaagga aaaacttttt tgtcttccgt ttcgttttca
       61 acaattggat gacattttga acataatagc atgagattgt ttggatcaag cttaagcagt
      121 tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc tctttccaaa tatgaactga
      181 ccacaccgct gacaatgacc accttctctt tcataaataa attgacgcat atctttccat
      241 gcttgcgttc gatagaatgg tttgttctca tgatgataaa cagactttgc ttgctgcttc
      301 ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt agataccctt tgctattttg
      361 ttcgtgcatc cgtcaaactg acagtatttc attctacttc acgaataaga tttatgatat
      421 cgccttttgc acggatagca ccaggaatat caataccatg tttcttagca tatgcacgca
      481 attcttttgc agtcatattg tccagttcat ccgtatcttc agtggactga tcattagtag
      541 cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct atcaagaatt tcactgctat
      601 gagctatgag ttcgccattg acagcaacaa atgatttgcc atcactcaaa attccttttt
      661 cttcttttac ttcaaaattt ggttcttgat ctttcggaac aactactgtc cttttctttt
      721 ctgaatccca atactctgtt ccactaatgg atgttctaat tttgatcatt gccattttaa
      781 ttctctcctt tgtaatttgt acggattact tttgcgccca ttcgctcata ccattcaatc
      841 gtttcctgca gattgggtag agtgtgtgac aataaagaaa taactaacgt attcattcca
      901 gttctgtaat catcattctc cacttgcaca tgtgcatgat taccattcca attttctctg
      961 tacggactat tctctgcttc aattgctttc ttataaactt tagccaaact tgattctact
     1021 tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt tactctccta tcagatattt
     1081 tgtggtatga tttataaaaa agatgcgagg tgttttaatg cttcaagaag ttaatattaa
     1141 gtatcgtgat cgtcaaaata cagagacaat ttatttgcca aatagttgtc cgcattgcgg
     1201 aaatataatg actccgtatg tgtattttgg ggtttcatca tatagtgcgt tcgataatga
     1261 tagaatcttt ggcgtattag tacagtgtat aaatgatgag tgcaacaaat tttatagttt
     1321 acaatttata aatacgttta atgaagatga aggatattct gaaaatattt taattaaaga
     1381 ttattaccgt ccgccgatta aaatagattt accagaaaat attgaaaaag tttctcctac
     1441 atttgttgat atttatactc aagccacaca agctaaatct gagcaattgg aacaaattgc
     1501 tggagttggt tatagaaaag ctctagaatt tctaattaaa gattatgcaa tatctaaaaa
     1561 ttcagaggat gaagataaaa taaaaaaaat gttattaggt aaaacaattg aaaattattt
     1621 gaatgatttt cctaaactcc aaagattagc caaagctgct acttggattg gtaatgatga
     1681 aacgcactat gttcgtagac atactaataa agatgttcat gatatggatg catttattaa
     1741 atcagcagcg caatttatcg cggctgatta tgatgctgat ttagctgatc aatttataaa
     1801 tggaaataac tttatgccaa ctactaatta aaagaataaa agagccacag ataccaatta
     1861 ataaaacggt tgggatatct tggctctttc atattttttt gacactaata gaatatcatg
     1921 ctgctaacaa tgtttacata gctacaatga ccgaacattt agtgaacagt gtaattttag
     1981 aataaagaga gttgttcttt gtacttctcg ctattgtaaa aatcaatttc gttttctttt
     2041 ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa agttcaatgt ctttctaatt
     2101 tctgcatgtc gctgtctgac atacgatgag ctatacccaa cagattcagc aatatcttct
     2161 aagctcattt gatcaacata tttcatctta acaatttgat tatcgatacc agaaaagcta
     2221 tctatcagca ataacatttc ttctttttgc tcgatcaaca attccaattc attttctatc
     2281 ttttggatat tctcttctaa agaagaagtt ctcgaattct tttcgatgcg gacgtttgct
     2341 aaatcaccac agacccatcg attcaattca agcttacttt tattaagatt ccactttaag
     2401 taaagaatct gctcatctag ttcttggtaa tcttttaacc aatgaaatct cacaaacgcc
     2461 accccttatg gtagaatagt attgcgctgc tatctcgaaa ggggtagctt ttttattttt
     2521 tcttagccgt tccattctgg attgcctttt gttccaaacg tcgttttttc ttcttaatct
     2581 ttgattttgt ttttcccata caattctcct ttccgaaaac acattttgtt cgatttttaa
     2641 tactaaataa aagttggcca gaatttttcg ttcgtcttta ctcatctttt tttgaataag
     2701 attactagca taaaaatagt tagtaccacg aaaatgaaca ggtaaatcaa ttacgttcac
     2761 cttcttcatg ccagttatca gccactagaa tctttgcgtt agtgccgcca ccgttttctt
     2821 ttttgtattt ttcaaatgct ttataagcag ctgtttttga tttatagatt tttccggaac
     2881 cgactgggaa catccatgtg ccctctgtga tagctaagat gtatactttc atttaattca
     2941 cccctttaat ccccaacact acatatccgg acttctgcat gtaatcagta atataagtga
     3001 tctctgcttt gtatgagtca ccagtgtaac taaatgttgg tctcgttagt ccaggtaatt
     3061 catgtttaat gtatttttta tattcacgta gtattaactg atcaccaact ttgaagtcac
     3121 gatcattctt gcggatctcg aattgtttgc gtccgctcgt cactgcttca aaatattctg
     3181 gtagtatttt tagttcgtgg atcattctgt ttcctccttc aaaattcgct catcatcaaa
     3241 aagtgaatct ccacaaaatc cttctcccaa atatttatcg ttcatgtata agtcaaccaa
     3301 atatttgtga tctgcttctc gatattcatt aatattaacg atctttattt tgtaaacatt
     3361 atcttcgtcg ctcactatct tgaattgttc tctgactttc accaccgttc ctcctctttt
     3421 tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag cttttttatt tgatataata
     3481 cttgtatcga ttaatcaaat cgaaattaaa tttaaaaagg tgatttatca tgaatatcaa
     3541 caatttagta aaactcgttg aacaacgttt taaagaatca ggattagaac aattaaaaaa
     3601 agataattct ccagaattat tttctaattt taccgaagac gacattccta ctcttaaaca
     3661 attaaaatat tattctgatt attggacgca aactcgtgag aatgaattaa atttagaaaa
     3721 actcattatt aaaaagaaat agcaactcca gcccacacgg ctggtttttt agtttcaact
     3781 tccctcctta cgggtcaaaa tcatcggttc cggcataatc tgatcatcct ttagcaacaa
     3841 atcaggatat tttttcctta gcttcttatt gttcgtgtaa gttttcaata gccattggtt
     3901 ggcttcgctt ttgctttctc cagaaaaata aattttccct gtttggatgc ctgcaagttg
     3961 aatcatcatt ttcacctcaa atcaaatttt tttctgctag atggagcaaa agcaatccga
     4021 catcaagaat cttttgaccc attatttcag ctactttgct cggcttaact ccttgagaaa
     4081 agagcaagcg tgcttgctct atctcttcgt gagtccaaat aaatttggct tcctcaagga
     4141 taattactgg atcagttgct ctcattgttc ctctggaaaa atgatgtcat tcttatgctt
     4201 ggaccaggta tctgcatatg gaacaaagta ttgtctagct aattcgattt gatcaattaa
     4261 agcagtttct gataattcat ggtctgcagc aatttcattc atactatcgc cttcatctaa
     4321 acagataaga ccgttgacgt tatcgatttt tacatttgtg actgcttgac tttctccacg
     4381 ctcataatat tctacagttg atactgatac atcagcttcg gagtttaatt gcagaatctg
     4441 ttactgccgg gataatttaa tattcttttc ataattcacc ttttttccta ctgacatcga
     4501 atatttttca tgttatgatt agccgtgaaa ggagagctat attatgtgtt tacacatgat
     4561 gtccttagct cattcggttc tttggaagct tttttggaaa taactctcag gttcttgacg
     4621 caaatctcat ttattctttg aaacaaaaaa cttccatttt ccgagagctt tggatcactt
     4681 caaaagagat ttgcatttat atttctgtga ccttgagtcg cgtaaagaac cttacagcaa
     4741 tggcggcaat tccactttca ggaaaccgcc ttttttcatt gatattggtg gttagcggac
     4801 tacccatttt ctttcggcag tgccaaacca caaatgatct gtgttgatgt ttaggtagtt
     4861 gtataccaga taagctaaat ccaaagtgcc tgcatcttca agacgacaat attccaaaaa
     4921 ttctcccata gctggacata aaaatcctgg agctatcata tcccaacagt taatagtgca
     4981 aagaaattcg tttagcttct gaacgttttc tttggtttca cgaattacaa catactccaa
     5041 ttcttctccg ctggcagagc attccaaaac ttcaaattga ttaagaaatg gtagaacata
     5101 tgcttcaaac atcaacattc catctttctg tttttgcact tgttttgcat caatcatgtc
     5161 tatcctccta ttcagttatt tcttccatta atggaattat ttcaggtcac tggacttaac
     5221 gaaaacacca ttgaccatct ttccagtccg acctttgatt tcgtcataag caaaatccaa
     5281 gcactcgtat aaatccatat cattttgcat tgccaagatg atcaaggtta ctactacatc
     5341 gccaattccg tctcttaaac catgttcatc ttttctagct agagaagcgg caacttcccc
     5401 gatttcttcg accgttttta acatttgctt gctgaaatcc gcttgatcca atcccttatc
     5461 ttttgcccac tgctctactt tcgcgattag ttcgtccatt atttctcctc cacataccta
     5521 aactgtcttc cttttgaatc aatccataag ctcctagctc tatcccaaat aatatttttg
     5581 ctcagaccag taatttcaga taactgttta gcggtacccg ttactagaat tcgatcacca
     5641 tgccagattg caatctttct cggcgttctc cgtttgggct tttcagtcca cattgattta
     5701 ccgagctttt ggacttctgc aactattttt ttatcttcct gccaatcttc tgaataagtc
     5761 aattcgataa ttcgctgcat tgccgctttc ttatccatcc cgacattctc ctttcagtaa
     5821 tttgagtact tgatcaagtg cgctctcacg tccgccatga aatgtgttga gccacttgtc
     5881 ttcgtacgac acactttgtc ttaaagcttc ttgatgcatt aattcgatct gtgctgtaaa
     5941 tgtctttagg tccatcttgt ttacacctgc tcaagttcat taagatgttt ttgcaagcct
     6001 ttaacacaat caatgaatag taatttggta tatgctaaat ttcttaattg cgttccatcg
     6061 atatagagtg cgaaatagta tctgagttta ctccaactcg aacgatcatt cttaattcct
     6121 tcaattccag cttcttcgag ttgatcatat acgtctctca ggacgtctaa ttcctcgccc
     6181 tttttataat tcgctatttc attaattagt tctagataat cgatcttcat ttttccacct
     6241 cttagaatgg tgcttttgat tgtctattag ctcgttctag cgcttttttc ttgagatagg
     6301 cttcttggtc gattgcccat tcaggaagct tctctcgtct tcctgtgcgc ttgtatccac
     6361 tgcttgcgtt cttaggttca cttttttctt tccttgccca acttcgaata gttgccaaat
     6421 agtttttata agtcttacca gatgattcac aatactctga cagtcgttcg attcgctctt
     6481 ggtaatcatt agggaattct gttttgagtt tctccatctg ctcatctgac aaaagaacat
     6541 ttttatactc tccgtattta tgacggacgg gcttagcctt cgattttttc gaaggcggta
     6601 actctcttat atattctttt gtattattaa atgtattatt aatagatgta ttattatctt
     6661 tgactttttc gtcaataggg gtattgcgtt tttcgtcaat agggtattga ttaattcgta
     6721 ggtacctatt gattatttga ttggtaccct ctttgtaaat gatttcccga ttcaagtatc
     6781 caaacttaat caaatcactt acccatcgcg atatggtctc tttattcaca ccatataaat
     6841 ctgcaaagta ctcattgcct gcccaacaaa agcctctttc attacacaag gccgttatct
     6901 ctccgtataa caacttagta tttggtttaa gtcttttgtc gtaccttacg ttggctggta
     6961 taatcgcata ataacttcga tgttctgtca tttttaccct ccaatattta actttttgat
     7021 tgttttctgg tttaatttga tccctttgat ttgatattta tttttgaaat taatcacacc
     7081 tattttgtgc ttctctgtgt gatggattct gcagagtgct gcaaatgtgt actctgcatg
     7141 atcaacttct ttgcgctttc gtcttcctag cgctttgtca aagtgatcga tgtcagctcc
     7201 tgttttgcca cagatgcagc agactctttt tgtgatgcat ttgtagaagt aatattcttg
     7261 attcgcaggt aaaatctcat agccttcttt gaaaggaata tgatgttcaa agatgaaatc
     7321 taagatgata tttgctaaga cattggcatc actcacagtt gtattcgatt cgtctttcag
     7381 gcttattttg cgccctgtga caccttcaaa acgaaagtag aagaattcct tccagaagtc
     7441 cgttggcatg cctgtatcga taaaaatatc gcctatcagc gcatagatga agtttcgttg
     7501 ctgtacggtg aaacgtctag gatcaataaa acgaatttca atgactcgat caccatcgta
     7561 gccgtcatac atcgtcttta gtcgatcaat gttcacttcc tcattgatgg ttgcgcttat
     7621 gtcttttcct ttgaactttt tcagaaccgc tgaatatgaa tcgattaatg gtttaaacac
     7681 tcatatcact tcttatctaa ttcttttctc ttagctgcta ttgctcgctc catcaaggca
     7741 cattgctcat agcttaactg ttcaatagtt tcaacgttat cagctaagag ccctaattta
     7801 tctgtctgct cattaacata ttcgattaag gttttggtca tatctttacc catctgctca
     7861 ttgaaagctt ctagaatcgt ctctagcatg tttaatttct ttgtatcgat tctaggtggt
     7921 gttggaatat cttccccttg aaatacatat aatcccagtc cgtgtagagc caatgctttc
     7981 acaaagcatc gcttcaatga gttattgatt tgcattgcat ttggtttaac aactggttgg
     8041 tttcgataat ctaaaacagg aaataattcg gtttccgtgt gtcctttaac cgttactgag
     8101 acagatacat aagtcccagt ttcatccata agaaaaggtt tatattcctc aacaagaaag
     8161 tcttgatgag ttccagaaac aaccctgtag tgtttgtact cattaatagt taccgttgcc
     8221 tgtggatcat tctttttcat aatctcccac gcgtgagccc aagataaata atcaaaattt
     8281 ccttttttct tgagaatttt atttaactta cgactaaaaa gtttttcaaa gttcgttgtc
     8341 cctttgattt cactcatcaa attctgcctc catttcagca atgtatttct tacctggtcc
     8401 gtaataagag atatcaatca agttatctct gtcgtactct tctagcgcat caatcaagcc
     8461 atcttcgatg acgtaaatat attcaggttt attcgaatgc ttcgatagat ggataagata
     8521 aacatgatcc caaatagtta catggttgcc taaatcatct tgatcccaag ctagttcttc
     8581 attcgtcaaa agatttcgtc tgatttttcg accacttgtt tcctcaattt tcggcttgcc
     8641 ccaatcagga tcaatcaaat attgatctag agtggaaagt tctttttcca tatgctaaaa
     8701 tctccttatg atgtgttttc tttgtgactc tttgcttgcc ggcggagtca cttttttatt
     8761 tgttgccatg ctttttgctt gtcaatatgt tgttggctta ggatgattgg tttatagtat
     8821 ttccaccagc aattagcaat t
//
LOCUS       DACBZN010000104         8811 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.104, whole genome shotgun sequence.
ACCESSION   DACBZN010000104 DACBZN010000000
VERSION     DACBZN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.104"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            72..1709
                     /locus_tag="IVD64_002354"
     CDS             72..1709
                     /locus_tag="IVD64_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6149572.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1854..2594)
                     /gene="truA"
                     /locus_tag="IVD64_002355"
     CDS             complement(1854..2594)
                     /gene="truA"
                     /locus_tag="IVD64_002355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6149573.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2785..3420)
                     /locus_tag="IVD64_002356"
     CDS             complement(2785..3420)
                     /locus_tag="IVD64_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6149574.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3567..4808)
                     /gene="sstT"
                     /locus_tag="IVD64_002357"
     CDS             complement(3567..4808)
                     /gene="sstT"
                     /locus_tag="IVD64_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP6149575.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5006..6002
                     /locus_tag="IVD64_002358"
                     /pseudo
     CDS             5006..6002
                     /locus_tag="IVD64_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6018..8036
                     /locus_tag="IVD64_002359"
     CDS             6018..8036
                     /locus_tag="IVD64_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP6149576.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8096..8788)
                     /locus_tag="IVD64_002360"
     CDS             complement(8096..8788)
                     /locus_tag="IVD64_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6149577.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT         2827 a         1657 c         1395 g         2932 t
ORIGIN      
        1 aatttttcat agcactagta tgatatacta gaaaagaatc aatttttatt acttttaaat
       61 aggagaaaaa aatgaaaaaa aacaaaaacc aaataataga tatcattttt atgttatttc
      121 taagttttgc ttacgtcatc cctctattca tgtctatggg aatctatcat agtgtcaatc
      181 aggatactta ctttcattta tctcgaatca taggactaga taatgtatgg agttctccag
      241 taaacttcaa taacttcgat catcatggaa caatgatgaa tattttttat ccatggctta
      301 ctctttatcc agctttttta ttttataaaa tgatgggaaa tcttgtttta agttataata
      361 tctactattt ttttatcact tttttaacaa tggtcgtttc ttatttttct atgaaacaaa
      421 taaaaaacaa tcgttatatt tcgctgttat tttcgattat ttatacattt tccgcctatc
      481 gtgccattga catattccat cgtgcttcat tgggagaagc tgtcgcttta acatttttgc
      541 ctttgatact aatggggtgt tacgaaattt atattagaga ttaccaaaaa tggtactggt
      601 tgtctattgg catgacgcta gtagtgtata ctcatttact ttctgtcgct atggtttctg
      661 tatttattgg aggaacgttg tttctttctt tttatttttg ggaccaaaaa atcgctagac
      721 tcttaagtct tttaaaagct accgtcctca cctttttttt aagtgcggga ttcttaattc
      781 cttttattca acaatctaga gcacaagaat tgaaagtccc actgggaaaa gaattaagtg
      841 gtatggctcc tagtgatatg ctgacacaca ttttaaataa taactataac aactatacta
      901 ttggattatt cttatttctc ggattgattg gtgcttttat ttttataaaa aaattaactg
      961 ctgatgattt atttattttc tttcttggtg tatttgttct tttttgttca accaatttat
     1021 ttccttggca actttttaac catacaccag ttaagagtct acaatttgtc tggcggttaa
     1081 atggtttttc ttctttattt atagcctata cgatgagtat agtgatctat tatatttttt
     1141 ctactaaaaa taattcatgg aaaataatgg tctttacgtt tcttgcacta atattgcatc
     1201 tctcaggagt aagcaattct atccatgcta acaaagattc attaacactt atagcacctg
     1261 aagacgctgt tgctatagct gaaaattaca atcatactga ttatgcaaac aaagagtcaa
     1321 tctatcatcc tgatatgatt aataatgatc aatatttact aggtaaccag gaaataaatc
     1381 caacaactca ttttatgagt aacaaactta caattgaatt agataattcg aataataaaa
     1441 atactgtctt gacgacacct atttatcgtt ataaagggca agtggcttct attaatggca
     1501 aactagtaca aacaaaatta tctaagtttg gcacaactga attaactatt cctccaggta
     1561 taaataaagt cgttattact tatcagtaca caaaactagc aattgcttca agatatttat
     1621 ctatcgttac tttgatttta tttttacttt atcgatttac tttttctaaa caaaaacaac
     1681 cgagaagaga agtacaacat tctcactaga tttttattta agaaagactt tttagctgta
     1741 cacataaaca ggctaaaaag tcttttttta tatttcttta atttgcttca tacattattt
     1801 aacatcgata tttatactgt gaaacatgcg tttcatccat tatcaaagta tttttatcga
     1861 taataaactt catccaaaaa cagaccttgt gcagggacgg tttctcctgc atgttgccgc
     1921 actttgcttt caaatatttc atcgattgtt tctatcggca tggcacctgt gccgatttct
     1981 agtaatgtcc ccatgataat ccgcaccatt ttatataaga atccgtctcc gacaaaagta
     2041 aaatgaagca tttctccctt tcgttcgata gataattcat caatcgtacg cactgtggat
     2101 ttttttgatt ttttcaatgc agaaaaacca atgaaatcat gtgtacccac taatttttcg
     2161 caagctgcat tcagcttgtc catatccaac tcttgaggga aataaaagct gtggtttcgc
     2221 tcaaatgctg aaggaatcac gttgttccaa acataatagc tgtatttttt gcccaccgca
     2281 ttatatcttg aatggaagcg ctcaggcatt tcttcgattc ttttgatgac aatatcccgc
     2341 ggaaggtaac gattcataaa gtcgatcatt tctgccagtc caatttctgt gtttgtttta
     2401 aaatttgcta cttgtcctcg cgcatgggtg ccagcatctg ttcttcctga tccaataatt
     2461 tctatctctt catttgtcat ctgatgcaat actttttcga tttttccttg aatggtttta
     2521 tcgtcatctc caagtctttg ccatcctgaa tatctttttc cgtcatattc gattgtcaac
     2581 ttgatgtttc tcatatttta gtggctcctc tgtcttccat ttggtcttct ctctaccttt
     2641 ttaagattgt agcacaaaat atagctcctt tcttctctaa aacagaagct atacaagaaa
     2701 actgtggaaa tatatatttc agaaaaacaa aaaacagcca ccggatgaag tatccgctga
     2761 ctgttcaatt tgacggctca agttttactg tttgagatgc aagatactca accaggcaat
     2821 gggcacataa ttaaagaaaa aactgattct ttggcaaaaa cctattttat tcagtaatgg
     2881 ccaagaattt atagaaggaa ttcttgccag tccataaatc gccgcaaaaa gcaaactgat
     2941 cacagacagt ccaaggtata agtggcttac gtccatttgc ccattttttc gatagatcaa
     3001 gaaaagaaga actggaaaca ataaaaaacc actgtaacct aaacctgatc cgatattatg
     3061 gacccatgtc gagacattcc acgaagattc atgggtattg atactgaaca aacctgtaaa
     3121 gatacaatca cctataccat agaacgcgat ggctgctgta actagggtag ctaacacacg
     3181 agatgtactt acaaatgcag catacaatgc aggcaatgac aggatgaaaa agatccctga
     3241 aaccacagac caaataagaa acgcttttct tacaggactg gaaacctctc caaatacact
     3301 gatcacgctc ttcatttgat ctaattcagg ataatacaat cctaatacgt acggagtcaa
     3361 aaaatcactc agcacgccta ataataaaaa gaaaaaacca aatttacgta aaaaatccat
     3421 gatacgcctc caataacaat tacctatgct tttaattgtt attataaacg actttatctt
     3481 gatttaacag aaacctctat ttacacgaac atctatataa ctcattaaaa actcgaatca
     3541 ttttaaaaat actaaatatc taacgttcaa cgttcactta atccatctaa acaatctttc
     3601 aatttgattt tttccccact tcttttacgc gcgcctagct ctcctattgc agtaaacaac
     3661 agatcacttg acgaatttaa ggcagtttcc attgaatcct ggataacacc gataataaat
     3721 ccaataccga cgatttgcat cgcgacatca ttagagatac caaacaaact acatgctaat
     3781 ggaatcaata acagtgaacc gcccgcaaca cctgaagctc cacaagcagc taaagcagac
     3841 aaaatacata aaaggacttt caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca
     3901 gtcaacgtca tcaaggaaat ggtaatcgcg gcaccgccca tattgatcgt agcacctaac
     3961 gggatcgata tggaataaga ctcttttgtc aatttcaatc tttcagccaa catcatgttt
     4021 atcgggatat ttgcggcaga acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa
     4081 caaaagaaaa tcaatggata gggattttct ttaataaaca aaaacgtgag gaatgggtac
     4141 acaactaatg cgacaaacag catcgtaccg atcaaaacaa gtaaaagctg cagatactct
     4201 gccaatccag cgattccagt cgtagcaatt gattgataaa ccaatcctaa aatccctatc
     4261 ggagcaaatg caataatcac ttgtacgact tgtgacaacg ctatagatat ctgatccacc
     4321 aactcttttg tcgattctga acgtgctcga aaagctagtc ccaacaatac agcccagaat
     4381 aagacgccaa ggtaattcgc atcaataaga gattgaacag gattttgtgt gacattcgtc
     4441 aacatagtag ataaaacact ttccaaactt tgcggtgctt tttcagatac tgtttcttgt
     4501 aaagtaatag gaacagtaaa aattgaactt acaataactg ctacaagtgc agaaagcaat
     4561 gttcccacta aatataagat caaaataggc ttcacgaatg tcttattccc aattttatgc
     4621 ttggcaattg acgccatcgt caagaaaaaa accagaatag gtgcgattgc cttcaagcaa
     4681 ctgacaaaca acgttcccaa tattgaaata aatgtccatg tggggaaaaa gataccaaac
     4741 aatattccga ctaaaatacc aacaacaata cgttgaacta acgacatatc caaaaacttc
     4801 tctaacaaat gaccaccctt tcacaatttt aacaatcatt gtatcatttt atgcaaagaa
     4861 actaaaggta taattttcta aaaattctaa caatacaaac aaccatttct tgctaatttt
     4921 gtactttttc tgtctacacg ttgttttttg agatattcta agttaggatg ttattaatgg
     4981 gcaacttatc aggaaacgtt ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt
     5041 cttgaaaaaa ataaagaagg agagttatgg cttcctgaca atctttgtcg cttgtataat
     5101 gatggtttta cagcgtatat tgatttcagc agtcactatt cctacatatg caacaaaccc
     5161 tggtcgattc tacatctata catttttaca aacttccgtt gttattggtg caaatttgat
     5221 cccactttac atgggttacc aatatcaaaa aatcgcccgt ttaaaagttt tacattattt
     5281 aagtcgtttt gcctttatat tcctaatagc cacactagtt tgcaatatct tttttttacg
     5341 tacaagctgg cagcttgaat attcgtgatt attggcttat cctaacacct atcagtcaga
     5401 attactttac ttatgctgtt tcttgtactt tgcttttttg tagtattcct tatacagttg
     5461 gatttttaaa taaattatcg aaaacaacaa ttaaatatct actattattt ttaacaattg
     5521 gattagttgg ctgctctact ctatttaata aagacccgtt tgcattacag aatggacaga
     5581 gtattttatg gattttttat ttatttttaa tgggttatgg tttgaaagaa tggaactgga
     5641 aagaaaaggt ccgctataag cttcctcaag tatttatcag cttgattgtt ttattcggtt
     5701 tgattatttt gatgacacaa atttcactca tcatcagagg aaatacagat acagcgctga
     5761 ggttttctaa accatattct ctcttcgcaa tgtattatac tttctcttcg tttttatttt
     5821 tagaaatgct atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt
     5881 tgattgtcac tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta
     5941 tgaaacgtga atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta
     6001 tttgctattt attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc
     6061 gtttcattca tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc
     6121 gttggaaaaa aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat
     6181 ttttttactg ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag
     6241 aaactatcca ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa
     6301 tcatcattat tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg
     6361 ccttgactgc taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg
     6421 aaatgcggga agagcctgtc tttccttcag atttaaaaat gctgaccgga ttgtcagaac
     6481 tattatcgat ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat
     6541 tgattactag tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt
     6601 ggaaacgtcg atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca
     6661 ttaatcacaa aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt
     6721 tctttaacca gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata
     6781 tcgacattaa aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa
     6841 tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca
     6901 atcagacgat cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc
     6961 tgacagtacc tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag
     7021 gattgatgtt atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga
     7081 caggcttgag tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg
     7141 agcgtcagaa aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc
     7201 cttttacagc tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt
     7261 tctattattt agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac
     7321 gcatatcgga tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacagaaa
     7381 cgtcacaatt catccaatta tccactatgc aaaaccatat gccttatgat gattactata
     7441 gtgaattgaa ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac
     7501 aaacttatat gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa
     7561 tcgacaaaat cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct
     7621 atagtgggaa tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt
     7681 ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta
     7741 ataactttgg tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt
     7801 tgttgacaga agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt
     7861 ctaatcgttt taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa
     7921 aaaatttaac tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc
     7981 ttgttgcagg taaccagtac agtgcaacat gggcgacaca aaaaataaaa gactaacaaa
     8041 taaactgagc aaggggttgt gataaaagac atgtcacaac cccttgttta tctcattaat
     8101 ttgcttgtcc ataaacgatt tgattaaagc catttttcaa ttcattattc gctgtctcgt
     8161 tgaagtaaag ataattatcg ggattcccct gattttgttg tgtcaccaat acttttggct
     8221 gattttgctg aaacccgaat agatagacgt gctttttcag atatggttct gtctctgcat
     8281 cggaatagac ggctgtgatt tgatagtctg cttgacctgt cccactttct gaccattctg
     8341 cagaaacagg cactccacca atagccatct tccactcccc gttcaaaata gcttgaggga
     8401 ctttaagacc atataagtcc acactcattt gattcgtata acttttgtat tgttgtccca
     8461 ttgtttggcc ccatttggtc ataaatgcct ctaattgtgc ggatttgtct gcattccaca
     8521 gtgtttgttc aacttgttcg ctgctttctg aagaggaaga tgaagcctct acagaagaac
     8581 ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat
     8641 ctgctgtttc tgtacttgct gcagttgact tgctctctgt gctagttgtt gtagtagttg
     8701 caactgttgc tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac
     8761 aaccagctaa caaaaaagct agaccaattg ttactaaaat cctttttttc a
//
LOCUS       DACBZN010000105         8801 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.105, whole genome shotgun sequence.
ACCESSION   DACBZN010000105 DACBZN010000000
VERSION     DACBZN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.105"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            171..515
                     /locus_tag="IVD64_002361"
     CDS             171..515
                     /locus_tag="IVD64_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP6149578.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IVD64_002362"
     CDS             516..935
                     /locus_tag="IVD64_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP6149579.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="IVD64_002363"
     CDS             complement(1027..1377)
                     /locus_tag="IVD64_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149580.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IVD64_002364"
     CDS             complement(1343..2674)
                     /locus_tag="IVD64_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP6149581.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IVD64_002365"
     CDS             2930..3496
                     /locus_tag="IVD64_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6149582.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IVD64_002366"
     tRNA            3641..3728
                     /locus_tag="IVD64_002366"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            complement(3780..4985)
                     /locus_tag="IVD64_002367"
     CDS             complement(3780..4985)
                     /locus_tag="IVD64_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6149583.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5123..5563)
                     /locus_tag="IVD64_002368"
     CDS             complement(5123..5563)
                     /locus_tag="IVD64_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149584.1"
                     /translation="MKFIKFGIIGLLVVTVMGGCGTSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVDTPLESDQ"
     gene            complement(5649..5849)
                     /locus_tag="IVD64_002369"
     CDS             complement(5649..5849)
                     /locus_tag="IVD64_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149585.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(5878..6300)
                     /locus_tag="IVD64_002370"
     CDS             complement(5878..6300)
                     /locus_tag="IVD64_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6149586.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(6317..6739)
                     /locus_tag="IVD64_002371"
     CDS             complement(6317..6739)
                     /locus_tag="IVD64_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6149587.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQQELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7028..7219
                     /locus_tag="IVD64_002372"
     CDS             7028..7219
                     /locus_tag="IVD64_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149588.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            7230..7496
                     /locus_tag="IVD64_002373"
     CDS             7230..7496
                     /locus_tag="IVD64_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149589.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALNQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            7683..7868
                     /locus_tag="IVD64_002374"
     CDS             7683..7868
                     /locus_tag="IVD64_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149590.1"
                     /translation="MEIIYFYKDEENNIPIVVEHTNNLSSSLLNTEEFNEMIKQFPEA
                     KNNLYVLVGRTEFKLNA"
     gene            complement(7857..8246)
                     /locus_tag="IVD64_002375"
     CDS             complement(7857..8246)
                     /locus_tag="IVD64_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HAP6149591.1"
                     /translation="MKLNQDCVRDILLLIEDEISFGSFLRLDFFLASEKLSKYDSETI
                     KYTLAKLDETEFLKSNTRWVNNDLASFSTGMITWKGHKFLDTIRDEKVWSNTKSIISK
                     FSSVSISMVESIASNVITQLIEKQLRV"
     gene            8299..>8801
                     /locus_tag="IVD64_002376"
     CDS             8299..>8801
                     /locus_tag="IVD64_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP6149592.1"
                     /translation="MQELVILKNKEAVTTSLQVAEAFEKKHKHVLEAIESVKRSVENS
                     ANVEDVLNFEKMFVEGNEPDTYGRSRRVFFMNRDGFSLLAMGFTGSKAISFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGG"
BASE COUNT         2953 a         1455 c         1583 g         2810 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatatcaacg attagacgcg atttcgatcg tgtctttttt tgttgtaatt
     3781 tagcctaaaa tgttttttga ctcccctttt gactccccct gagtactatt cttcataaat
     3841 gctacaaaat tattgatgac ttttttgttt tgagtttcag ttacatgagt gtaaatattt
     3901 gaagtagttt gaatgtctgc atgccctaaa cgttcctgaa catcttttaa agaagctcct
     3961 gattcaaaaa ggagagaggc atgagtatgt cgaaaaccat gagtagtgat tcttttagtc
     4021 aattctggat gatgcttata tatgacatta agccacgaac gtgaaactgt agggttatag
     4081 agaagaccat catggtgaaa aattaagttt tcgctttgga ttgtaacgat cgttttgttt
     4141 tttgtataat actttctcaa taaactgatc aaatcattgt ccagataaat ttttcttttt
     4201 ccagattttg atttaggagt atttacgatc aatctccctt gataacctct tgttacagtt
     4261 ttattaatat tgagtgtttt gctcttaaaa tcaatatccg accaggtcag tgcaaaagct
     4321 tctccttttc ttatacctgt aaaagccaat agggaaaaga ataaatatct ttcatcatca
     4381 tcgtttctta ttgcttctaa aaattcgatt aattcttctt tagtataaaa ttcgatattt
     4441 ttatcttcga tatctagagc ttctccacgt ggaatagaaa ctaatttcat aggattgcta
     4501 gttataattt gtaaagaagc agcgtaatca aaaacattag atgtgtaatt ttttattttc
     4561 ttaaaaattt taggatggct atcggaccaa gtgtttactg cttcttgaca gaaaaaaacg
     4621 tctattttgt caatgaactt atctccaaac acttttaaga tgtgtgtgtc aaaaatttgt
     4681 ttggtggagg accaagtgct ttctttaact gtctttttat aattttcaaa ccataagccg
     4741 taaacatatt cgaatttctt gctcttttga gctgtagaag cttgcagtcc tttttcctgt
     4801 atatctgcct ccaatctttt taaagctctc tcagcggcta atggcgtact aaaacctcgt
     4861 ctcgttgttt ttcttttttt tcctgttagc ggatcaactc ctaagtacag actaaactga
     4921 tatttttctt ctcctttttt tgttaagtgc tttttaattc gtttatcaat ttcttttttt
     4981 gccatctttt atctttcctt tcgtacgttt gttcggttgt acagcggatt tcgagatggt
     5041 aaaatagggt acaacaaata gacctacttt accgtagctc tttgcacatt tgcgttcttg
     5101 gtcgggcggc aaatgtgctt ttttattgat cgctttctaa tggtgtatct acacctttat
     5161 tagggtctag attgctatat agatatagga cggtataatt agttgcatct tttttcgaat
     5221 aaatcaaaga aaagttgtat tttttgtctt gccaagtaaa atctccagta ccattgtaga
     5281 catttttgta ctcttcacca gtttctgcat ttttatcgtc aggaaattga gtaactttga
     5341 tagattttaa atccatcttc aaattatcta ttgaatatat ttctttaatg tgtttctcgg
     5401 caatagttgc aaactcagcg gtttgatcag agtctacttc tttgatagta ctgtttgtct
     5461 cactataatt ttttgattcc tttgtttcag ctgttgatgt tccacatcct cccataactg
     5521 tcactactag taatccaata attccaaatt taataaattt cattttctac tccttatctc
     5581 tatgatatta ttttattggg gaatcttaga aataaggttt cgagtccgtg ttgctgcacg
     5641 ggcttttttt atttaaataa gtcccaaaag ctaaacgtag tctttttata aactttattg
     5701 taagcagcct tttttgggtc tttaatccag ccgctacctt ttttcccata accaggaata
     5761 actgcttttt ttacttttct ttttgctttt ccagtggttc tagcgcttat ggattttttt
     5821 atacttggtt ttctcattcc tattttcatc tatatcctcc ttacgttgta ttataattca
     5881 caagaaacga tcgaattcat ctggtaatcc aaagcagacg agcttttcat attttgtcat
     5941 atgatcaaag ccgtctaaag ttggatcaat agtcagatga gttgcgaaat aatttgcttc
     6001 tttttcaatt tttaattcag atactagcgt ttttttagac aacataggtg tgttttcatt
     6061 cggatgcaaa acacagtggc ctaattcatg actacaagtt atccattttc ccatctcatc
     6121 taaactagaa tttatgtgga tcatttttat ctgtttgaat ttactatagt aaccataaat
     6181 ttctcccaaa tcttcttcta aaactagtat cttcaattct cgtgcaattc taaaaggatt
     6241 gcgtgtttga tatagtttta ccagcttatt aattttgtta tcaatttgag gtaaatacat
     6301 aaacaacgta ctccttttaa tcgcgatatt ttttaggtgt gtattttttc ttagcttcta
     6361 ttttagctat tcgcaaagca ttctctaaag aaataatgag agcttcacgt gtcgcttcac
     6421 tcatttcatt atctttcttc gaaaaagcca ttccatcagc attagataaa tcgtcgatta
     6481 atgactgtaa ttcttgttga atatcacgct cgtctttttc tgttaaatca tagtatcttt
     6541 ttttctcagt acgtcctagc aaataatcta cagatacgtc gaagtagtca gcaatttctt
     6601 gtaacttttc ggcagttggc tgttttcctg catttaaact ataaaagtaa ttctctccgt
     6661 acttcaaatc tttagcgact tgttgaaggt ttttattctt gtttttcgct agttctttta
     6721 ttctttcaaa tgttgtcatt ccaacgttcc tttccaacac gaaaaaataa tttacgaaaa
     6781 agagtaaaaa tagctttaca attttacgat atagagttat tatttatccg taagttaatt
     6841 tgatagaaaa aagcaaacta aaaacacacc ttatagcata aagtttggcg accaagtgca
     6901 aataaaaggc tttgttatag gcttatttct ctatgtattc attttactct atagtgtaaa
     6961 aatgtcaatg gaaattatat ttttctatca ctttaactta caaatttata aaaaaggaag
     7021 tgggttaatg tcaaacttgg ataacggtcg tgcggcgatc aaagaattta tgaaagagaa
     7081 tggaataact atggatgatt tggctactgc ttatggtcac acgcgtgtac gaatgcaaca
     7141 aattcttgat ggacactgga gtggaccaaa ggcaaacaga actattctag agatcatccg
     7201 tgattacaga attcgatagg aggctagtca tgaatattct aagcgaagag tttctgactc
     7261 gtttgagaat tgcaattgtt gaagttgtga aggacgcact taatcagctt tcaaaaaaga
     7321 atctttcaga aacacgatat ttgaaaaaaa tcgaagctag aaaatatgtg ggaggtgtaa
     7381 acgatcaagg ctttgagaag ttaatagctc acggtttaaa agaaattcgt atagatggct
     7441 ttttgagata tgacaaaaag gacatcgatg aactgatggc taaatacaaa atttaaagga
     7501 ggaaaaacat gggaaagttt aatcgaacat tagtattcag cgcaccgcta atcatctacg
     7561 ctttaggact ttggggaaat agacaagcgt tgataggaac gatcgtttac atggtttgga
     7621 tttttatagg gctggatgaa gctgagtata gagcgaaaaa gccagtcggg agggactgac
     7681 taatggagat tatttatttc tacaaagatg aagaaaacaa tatcccaatt gttgtcgagc
     7741 atactaataa cctatcatca agtttattaa acactgaaga attcaatgaa atgataaagc
     7801 aatttcctga agcaaaaaac aatttatacg tactggttgg aagaactgaa tttaaattaa
     7861 acgcgtaact gtttttctat aagttgagtt attacattgg atgcgattga ttctaccatg
     7921 cttatagata cgcttgaaaa tttagagata atcgattttg tatttgacca aactttttca
     7981 tcacggattg tatctaaaaa tttgtgacct ttccacgtaa tcattccagt agaaaaagat
     8041 gctaaatcat tatttaccca tctagtattt gatttcagaa attctgtttc atcaagttta
     8101 gcaagtgtgt attttatagt ctcactatca tatttcgata gtttttcaga ggctaagaag
     8161 aagtctaatc gtagaaaact tccaaaagaa atctcatcct cgattagaag gagtatgtca
     8221 cgtacgcaat cttgatttaa tttcattata aacaccacca gtttttacct aaattatacc
     8281 aaaaggagag aaataaaaat gcaagaatta gtaattttga aaaataaaga agctgtgact
     8341 acgagcttgc aagtagctga agctttcgaa aagaaacata agcatgtgct agaagcaatt
     8401 gaatcagtaa aaagatcggt cgaaaattcg gccaatgttg aagatgtgct caattttgag
     8461 aagatgtttg tagaaggaaa cgagccggac acgtatggga gaagtcggag agtttttttc
     8521 atgaatagag atggattttc cttgttagct atgggattca ctggaagtaa agcaataagt
     8581 ttcaaactca aatttattga agcattcaac gagatggaag atgtcattcg taaaaatact
     8641 gttcctcaaa cgattgaaga catgatgatc taccaattag aagaaatgaa agatgtaaaa
     8701 aaagatgtct ccatgcttaa agatactatg cgaattagcg gacaacaaga gtttgaaatt
     8761 aagcaaaaag gaaatatgaa agttatggaa gttttaggag g
//
LOCUS       DACBZN010000106         8722 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.106, whole genome shotgun sequence.
ACCESSION   DACBZN010000106 DACBZN010000000
VERSION     DACBZN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.106"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            7..2199
                     /locus_tag="IVD64_002377"
     CDS             7..2199
                     /locus_tag="IVD64_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP6149593.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="IVD64_002378"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="IVD64_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP6149594.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2670..3071
                     /locus_tag="IVD64_002379"
     CDS             2670..3071
                     /locus_tag="IVD64_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP6149595.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            3086..3985
                     /locus_tag="IVD64_002380"
     CDS             3086..3985
                     /locus_tag="IVD64_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP6149596.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="IVD64_002381"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="IVD64_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP6149597.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            5324..6241
                     /gene="glyQ"
                     /locus_tag="IVD64_002382"
     CDS             5324..6241
                     /gene="glyQ"
                     /locus_tag="IVD64_002382"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP6149598.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            6243..8318
                     /locus_tag="IVD64_002383"
     CDS             6243..8318
                     /locus_tag="IVD64_002383"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP6149599.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2943 a         1560 c         1853 g         2366 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaagaaa aacttggaaa ggcagcattg
       61 cctttcctgc tgttcttgtt ttttatcatt atctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa
      181 acgatcgaga acacggaaga aaccgaacaa aaaagaaaac tggcagcaga agcagtcact
      241 ccagaataca cttaccaaaa agatcttgca gatgaccaga acaatcggat caagcaacta
      301 tttgaattga tcgataaaac gaatgatgcc atcaacaaat cttatgacga aaagattgat
      361 aaggcaaagg aagatgaaaa tatcccaaag cctactacag aagagcggat cgccgcgttg
      421 aagaaaaatt ttgaaaatgt gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaactgaga tccatactgt gagggatgag
      541 agtctgaagc tcattgacga acagatgagc aaacaagttc gtgaaagtga attggaagct
      601 tttaaacaag aagcagaaga gcaaatccaa tatttgaatg tcacaccagc acaacaacaa
      661 atgattcgct acttagtgga tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag
      721 acagaagaat tgaaacaatc cgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaatcattg ttcgtgaagg taatcaaatc gatgcttcag ctatgaaaaa attggaatta
      841 ctaggactga ctaatcaaac aacttcgatt tttccgctaa ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggcgtact gatttataat tcacttcaat tccaaaatgc aggagaaaga
      961 acaaaatacg tgcttttcta tgtgacagct atgtcagtca gcatagtgct gatgaaattt
     1021 ttccaattat tccaaacgga acaagctact tatatcccgc tgttttatcc cgctgctttt
     1081 gcaccacttg ttttaagctt tttcttgaac cgaagagcag ggatcatggc tggtattttc
     1141 caagcagtat ctgcactttt cattttttat gattcgattg gcacgaactt tctaacgatt
     1201 atcttgatgt cttatgtttt ctctgggctc atggcaacga ttgtcagacg caaacggatc
     1261 tcagaacaag gattatctgc tgccatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatcg tctatcaagg aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc
     1381 tgcgggttca ctggtgcact cttctcattc ttgctgacga tcggtctaca cccatatatt
     1441 gaattgatgg tcaatgatga cagtgtaatt gtattaaatg aactcagcaa tccaaatcat
     1501 cctttattga aacagttgct ggaagaagcg ccaggtactt atcatcatag tatgatggta
     1561 gctaatctaa gtgctaacgc tgtagcagaa atcggcggac gttctttgct aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacatgcta gctttttcgt tgagaacttg
     1681 ccttcaggag cggaaaaccc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg agccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcaaacagca tcatggaacc acgctaatgc gttatttcta catcaaagcg
     1861 aaagaacgca atccggaagt aacagaagag caattccgtt atcctggacc aaaaccgcaa
     1921 agtcgtgaag cagggatcgt caatattgct gacagttgtg aggctgcagt acgggcaatg
     1981 gatcatccga cgagcgaaaa aatcgaaagt tttgtccata atcttatcga agagcgtatt
     2041 tccgatggtc aattagacga tagtggactg accttgaaag aaatccgaaa agtggaaaaa
     2101 tcattgatca gcggtttaag tagcaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 acagaagttg agaagataaa ggaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattaccttt atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgatga
     2281 cttgctgcaa tttgcagcat cttatttaaa gctgccagag gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcggcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tgcctcgtgt actaggagac atcatgattt ctattgaacg
     2521 tgctgctgaa caagcagaag cttacggcca ttcatatgat cgggaacttg gctttttagc
     2581 ggtacatgga ttcttgcata tcaacgggta tgaccatatg acaccagaag atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttgga tgcctatgga ctcaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaatatg aggaaacatg ttgcgttcgg tatccttgct ttatttatgg
     2821 gcgtgatttt ccagcttgat cggatagagt ggttatggct gcttttagct gtttttctcg
     2881 tttggatagt agagatcatc aatactgttt ttgaaaatgt agtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt tgctgtcatc attgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaaccga atcgtgggtc aaaaaattgc
     3181 cattatgagt gacaaggcac agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ctgattttta tcgatacacc aggaatccat aaaccaaagc atcgtctagg
     3301 agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggatgcag tcttatttat
     3361 gatcagtgct gatcaaaaac gcggccgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct ccagtttatc tgattatcaa taaaatcgat aagatccatc cagacgagtt
     3481 gctgggaatc atcgaagatt attcgacgca gatgcctttt gcacaagtga ttccgatctc
     3541 tgctacagaa ggaaacaatg tcgaacgctt aatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact ttatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggccacgat
     3781 catcgtcgaa agagatagcc aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa
     3841 gcaagtagga acgaaagcaa gaaaggatat cgaaaatctt ttaggagaca aagtatttct
     3901 agaactttgg gtcaaagtcc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 tggctaccga aaagacgaat actgatattc tgaacttctt ataggttaag tatagaaata
     4021 aactggataa aaatccgcac gttgagaaaa acggcggaag atcgaaacat aaataatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcgaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaaggaatc catcggcaaa
     4261 atcagccatt agctccggct ttgctcccct ttaccgaagc agtctatatc ggtaattttc
     4321 gaagtgaagg tttgtcgttt ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc
     4381 aagaagatat ctttttgagc ggttatggta cctatttgct aaatctagta gatgcagcaa
     4441 tcgaagatca tcaatatgac ccgcatttat tccaatttac gcaacaagct ctgcagcgga
     4501 tggatcaagg agatgatgca gaaatcatca cgaatatctt tgaagttcaa atactgcaac
     4561 gcttcggaat cgcgccaatt tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatct ggcggtgtga tatgcgagaa acactggcca atggacgaac
     4681 accgttatca tgcagatcct cgcgctgttt atttcgttcg aatgttttcg gctatttctt
     4741 atgataagat cagtggaatc cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca
     4801 ttgaccagtt gtatgatgaa tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga cgccagagaa aaatatgcct gaaaaagaga
     4921 tacctgaaaa agaagaataa atgaaagata caacaaaaaa atcaaaacta tttgacaaat
     4981 acagggactt ccgtctatta ttaaccgtag attaaataga tcgttcactg agaggatcta
     5041 ttatataaac aagaaaactg caaaatgaat acgtacattg accaagatca gtaaaaaaca
     5101 cgtggtttag cgagttcggg agagtggaag ccggacccaa acgatttttg aagaggcact
     5161 tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa
     5221 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
     5281 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
     5341 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
     5401 gcaagcttat gacacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
     5461 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
     5521 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
     5581 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgtcttt taggaattga
     5641 tccattagaa cacgatattc gttttgttga agacaactgg gagaacccat cgatggggtg
     5701 tgccggtgtg ggttgggaag tatggcttga tgggatggag atcacacaat tcacgtactt
     5761 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagatcactt atggactaga
     5821 acgtcttgct tcatatattc aagaagttga aagtgtctat gatctggaat gggctgatgg
     5881 tgtgaaatat ggcgagatct tcaaacaacc agaatatgaa cattccaaat attcttttga
     5941 agtaagtgac caagacttgc tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg
     6001 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gcagccatac
     6061 attcaattta ttagatgctc gcggtgcagt atccgtaaca gaacgagcag gttatctttc
     6121 ccgtatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt
     6181 aggattccct ttattgaata aaaacacagc aaatccagaa aacatgaagg aggctgaata
     6241 agatggcaaa agacttatta cttgaaatcg ggctggaaga aatgcctgct catgttgtca
     6301 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     6361 cttatgatac gatcgaaacg ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca
     6421 ttccagaacg tcaagcagac agtgaagaag aagtcaaagg accagccaaa aaaattgctt
     6481 tggacgcaga tggaaactgg acgaaagcag ctgaaggatt tgtccgcgga caaggtctga
     6541 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     6601 acactcacgg caaagaagcg aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     6661 tcactttccc agtaacgatg cattgggcag aatacgattt tgaatatatc cgccctattc
     6721 actggatcgt ggcattgtta gatgacgaag taatcccgtt tgagatctta gatgtcaaaa
     6781 caggcagagc ctctcgagga catcgtttct taggcgaaga tgtgacattt gcgcatgcaa
     6841 aagaatatga agaaaaattg aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag
     6901 aaatgatcgt tacacaagca aatgaattag ccgaaaaaaa tcactgggtt attgatctag
     6961 acgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc ttttctggcg
     7021 acttcgaaga aaaatatctt tctgttcctg aagaagtact agtcacttct atgaaagaac
     7081 atcaacgtta tttcgaggta cggaacgatc aaggtatgct gcttcctcat tttatttctg
     7141 ctcgtaatgg tgatcgggta aaattggaaa atgtggttcg aggcaatcaa aaagtattga
     7201 cggctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     7261 aatacgttga aaaactaaaa actgttactt tccatgaaaa aatcggtagt atctatgaga
     7321 aaatgcagcg ggtacaagtc attgcccgta tcattggcga acaagtagga ctatctgagg
     7381 aagaattgat ggatctgcaa cgagctgctg aaatctataa atttgatcta gtaaccaaca
     7441 tggtagggga atttcctgaa ctacaaggga tcatgggcga aaaatatgct ctgctgaaag
     7501 gcgagaaacc tgctgtagca acagcgattc gtgagcatta catgcctata tccagtgaag
     7561 gagagctacc tcagtctaca gtaggttctg ttttagcgat tgcagataaa ttagatagca
     7621 ttttttcttt ctttgcagtt gggatgattc caagcgggtc aaacgatcct tatgccttac
     7681 gtcgccaagc atacggtgtc gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt
     7741 ctgttctgca agaaacgatt agtgatgtta tcagtaaaga tacggatcgt ttcggcattg
     7801 gtttatcaga agggcaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     7861 tgacaacgaa gaatattcgt catgatgtca tcgaagctgt tttgaatgca gaacaaaaag
     7921 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     7981 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     8041 aaaaacaatc agaagtagac cctgaactgt tcgaaaatga agcggagaaa gaactgcaca
     8101 aagcagtaga ggcaatgaaa aaaagctttg ctgaccaaac gatcaacgaa aattatgatc
     8161 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     8221 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     8281 tgtcgctagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     8341 gagaataggc acaaaatagg ctatttctct ggtttatttg ttataaatat aaataactat
     8401 acccgtggtg ctagttaatt ttttcctagg tattatacaa gtaatttgag tcggtcaatt
     8461 tcaactgaat tatctaaact aactgatcta cttttagata attcagttga aatttattgg
     8521 taaactgtcg cttttctttt taaatggcaa aaatttttaa taatatatga caatttagaa
     8581 atcaactata agactgatta attcaaaatg atcttagaat cattgtatga acactaaata
     8641 ctatattgtt ttctattaat cgttatataa acgattatga attcaaagct actttttatg
     8701 acgtcaatca gagagtgtaa aa
//
LOCUS       DACBZN010000107         8627 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.107, whole genome shotgun sequence.
ACCESSION   DACBZN010000107 DACBZN010000000
VERSION     DACBZN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.107"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVD64_002384"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVD64_002384"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP6149600.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IVD64_002385"
     CDS             complement(1098..2201)
                     /locus_tag="IVD64_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6149601.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IVD64_002386"
     CDS             complement(2291..3226)
                     /locus_tag="IVD64_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP6149602.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IVD64_002387"
     CDS             3528..5462
                     /locus_tag="IVD64_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6149603.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IVD64_002388"
     CDS             5919..6335
                     /locus_tag="IVD64_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP6149604.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IVD64_002389"
     CDS             6349..6915
                     /locus_tag="IVD64_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP6149605.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IVD64_002390"
     CDS             6938..7924
                     /locus_tag="IVD64_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6149606.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8627)
                     /gene="scm"
                     /locus_tag="IVD64_002391"
     CDS             complement(8079..>8627)
                     /gene="scm"
                     /locus_tag="IVD64_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6149607.1"
                     /translation="SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDS
                     TDTSTTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDST
                     DTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITL
                     AGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2529 a         1664 c         1758 g         2676 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt ggtagaggta gtatctgtac tgtcacttga agctgacgtg
     8461 tccgtgctat cacttgttgt ggtagatgta tccgtactgt cgcttgaagc tgacgtgtcc
     8521 gtgctatcac ttgtggtaga ggtagtatct gtactgtcac ttgaagctga tgtgtccgtg
     8581 ctatcacttg tggtagaggt agtatctgta ctgtcgcttg aagctga
//
LOCUS       DACBZN010000108         8493 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.108, whole genome shotgun sequence.
ACCESSION   DACBZN010000108 DACBZN010000000
VERSION     DACBZN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.108"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..173)
                     /locus_tag="IVD64_002392"
     CDS             complement(<1..173)
                     /locus_tag="IVD64_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6149608.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVD64_002393"
     CDS             complement(593..1714)
                     /locus_tag="IVD64_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP6149609.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IVD64_002394"
     CDS             2042..3451
                     /locus_tag="IVD64_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP6149610.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVD64_002395"
     CDS             3448..4176
                     /locus_tag="IVD64_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6149611.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVD64_002396"
     CDS             complement(4304..5215)
                     /locus_tag="IVD64_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6149612.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVD64_002397"
     CDS             complement(5232..6239)
                     /locus_tag="IVD64_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6149613.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVD64_002398"
     CDS             complement(6236..8365)
                     /locus_tag="IVD64_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6149614.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IVD64_002399"
     CDS             complement(8370..8477)
                     /locus_tag="IVD64_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149615.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1487 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg gtt
//
LOCUS       DACBZN010000109         8478 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.109, whole genome shotgun sequence.
ACCESSION   DACBZN010000109 DACBZN010000000
VERSION     DACBZN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.109"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            61..816
                     /locus_tag="IVD64_002400"
     CDS             61..816
                     /locus_tag="IVD64_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6149616.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            813..1637
                     /locus_tag="IVD64_002401"
     CDS             813..1637
                     /locus_tag="IVD64_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149617.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            1926..4037
                     /locus_tag="IVD64_002402"
     CDS             1926..4037
                     /locus_tag="IVD64_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6149618.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(4075..6132)
                     /locus_tag="IVD64_002403"
     CDS             complement(4075..6132)
                     /locus_tag="IVD64_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP6149619.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(6246..6458)
                     /locus_tag="IVD64_002404"
     CDS             complement(6246..6458)
                     /locus_tag="IVD64_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149620.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            6645..7112
                     /locus_tag="IVD64_002405"
     CDS             6645..7112
                     /locus_tag="IVD64_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149621.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            7109..7351
                     /locus_tag="IVD64_002406"
     CDS             7109..7351
                     /locus_tag="IVD64_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149622.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            7369..7671
                     /locus_tag="IVD64_002407"
     CDS             7369..7671
                     /locus_tag="IVD64_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149623.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            7743..>8478
                     /locus_tag="IVD64_002408"
     CDS             7743..>8478
                     /locus_tag="IVD64_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149624.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNE"
BASE COUNT         2907 a         1312 c         1643 g         2616 t
ORIGIN      
        1 ggagcagggg gactttctgc agttgtttac tttaagaaga aagcctagaa ggatcagcta
       61 atgattattc gtaagccccc aaaaagaaga gtcatgcaca aactgatgat atttttttgg
      121 ttgctgtcgg cagtagcagt ctatttaatt ccagttgtgg aaacaaatgt agcgaaattt
      181 caacaacagc aaagaatttt agccattgaa aatcttgaaa aaacagattc ttctttggat
      241 gttgcgaaaa tagagttagg cgatcctgtt ggtgttctta caattccaag tatttcttta
      301 aaactaccaa tttatgacgg aacttccgac aaaattttag agaatggtgt agggattacg
      361 gaaggaactg gcgatattac aggaggaaac ggtaaaaatc ctttaatcgc ggggcacagt
      421 ggactttata aagataatct tttcgatgat ttgccttcag taaaaaaagg gcaaaaattc
      481 tttattaagg taaatgaaga acaacatgcc tatcaaattt atcgaattga agaagttaaa
      541 gcagaagaat tacaaaaaaa ttatttagat tatcttatgc ctgaaaaaga ccaagatagg
      601 atcacgttaa tgacatgcac accaaaaggt gtcaactcac accgcttttt agtgtatggt
      661 gaacgagttt ccttcagtaa agaagattta aaactacaga aaaaagcaaa aaataggaaa
      721 tcaatttcat tggattggcg attagcactg ctattcgctt ttttttgcgt tcctattatt
      781 tttatttata aaaaaataag gaggagatcg gaatgaataa gagaaaatgg ctgtttgttt
      841 gtgtagcagc tctcttttct actgtagctt attcgcaact gtctttcgct gacagcagtg
      901 atagtagtac cactcaaaca atagataatt caattactgc accaagcgaa acggacagtt
      961 cggagaatac tacagaagaa acggcagatt caaatataag ctttactgaa tcaacgaatc
     1021 caacgtcaga aagttcttca gagacttcag aaaagccgca agacgtagaa gatacgcaag
     1081 tgatcaagca agccattcat attcaaaaaa ttatttcttc gaatcaaatc aacaatcaag
     1141 gcagtcaagt agcccagcaa gaaggtatca atggcgcgaa gtttattgtc tacgatatca
     1201 cgaatatctt agaagaaatg tcacaagatg ataatgagaa aaatcaatct gtagaaataa
     1261 ttgagtcaaa attgaaagat cgagcaaaaa aactttcata cgaccagttg aaaaaagtgg
     1321 ctgaaggaga aaccaaaacg attgatgatc aggaaggcgt gattgaattt tccattgaag
     1381 tacctgcaaa caaaaaacaa gcatactata tcgttaatga atcaagccct gaaaacattt
     1441 caaacagtga agatattgtt ttattgacac ctgtttcaaa tgaaaaaggt gaatttttga
     1501 cggatgtttg gttataccct aaatcagaga aatcagagcc gaaagaagaa attaaaaaag
     1561 ttgtgtcaac tggtgtaaag aagaactttt ttgaaaattg ttgggaattt tttgctcaat
     1621 tattttttga taattaggat gaaatgatga aaataataag aatattagtt tttaatctat
     1681 cctttttagc cgcaacaata ttgtgttgcg gtttaatttt ttctcaatta tcaatttatc
     1741 caagatatca acgattgttc attcattttc taaacattca atgtgcttcc atgccattag
     1801 aggtgattaa aaagctcttg atcaatccaa tttttatggt tagtttggtt cttcttacag
     1861 gattggtaac gtgggtatta ggaaaaaaag aatgaaaagc ttacgatcgt ttaaaaaaat
     1921 attagatgga gggcattatg gaaaataaaa ttcattgcac tacgggaaga gtaattactt
     1981 tagtgttgtt tttgttcctt cttctttttg cgaggggaac tgcagtccag gccttagaaa
     2041 aagttgatcc gagtcaaatc aaagaaaata ataatctggt gctttatcat gatagtgatt
     2101 cattgagaaa tatcgaagta actgggatca atggatttaa gatactagat gagtattcaa
     2161 ctaaaaaaca aaaagtactt gcttggccaa aagtcaaaga tttgaaaagt acgtcaaaaa
     2221 tcgttgccca ttatcaaaaa gtaggtactt atctaggaaa agaaattgat tgtgacgtga
     2281 ctttttctaa ttttgtagcg cacgctgggg ttaaccaaga cggattagct aaaacaatta
     2341 atggcaaaaa gtattataat tatttgctct ttgataaaaa tatttatcga ggatttttca
     2401 tgcagtcaat caatgtcatg aatgcaaaac tagaatttaa gtatgcagga acaaaaacaa
     2461 aagtagcttt gagtgaacca cagccagaaa ttcaatcaaa aatgagatcg atgagagcag
     2521 ctggtattga tttaaacgat acatttctat ccatttacag tttaaatgga cagtatcagc
     2581 aacgtgtgtc ttggtataat gacaataatg aatctgtcgg tgaacgtaat attgatatga
     2641 gagaatttgt tgggtatgaa aaaatgggta gcttacctta ttttgtcaca acagatacag
     2701 catgtgcaga atacaaagct cctgcgttat caacaaacaa tttaacttca aaagtagtgg
     2761 gaggacgtgc agaaaaggct tatagctcga atgatcattt caccgatgtt gtaggagctg
     2821 atacttatca cagaagtggt gtaacgtata cgcttcaagg cgcttcccca acattcatga
     2881 ttggcgcaaa tacgaatagt atgatgttta gctttgatac tgcattgcta tggacaccac
     2941 aaccatcgaa gcctacaaaa gaagtgttta acaaagctaa tactgaagag gcagcacaca
     3001 atattgacaa aaaagtgatt ccacaaggat cagatgttta ctatcatatt caccaaaagt
     3061 ttgatgcatt aacagtcaac acaatgaaca aatacaaatc atttaaaatc actgatacct
     3121 ttgacagcaa aaattttgat atggtatcgg atgggaaaaa ctatgatggc gcattgtggt
     3181 atgttgaagc gaatggtact tggaaaaaag ctgatacagg aggaaagtta actgtatcag
     3241 gtaatacagt cacttatact gcttctgaca gctttttagc taacgctgca aattacggta
     3301 gggattatat tttgcagctc catatgagag caaaaaatga tgcaaaagga attgcaccaa
     3361 atgtggctac tgtaacgttt aacaatgatc cgaacaaagg aacacaaaaa acaaatacag
     3421 tagaaaacta tttctctctt aaaccaaata aaaaagtgga acaagatggg aaagatgttg
     3481 atggtcgaaa tgatggtaaa aaaggaacgc ctacagctcc tttaaatgca ggatctgaag
     3541 tacaatatct agtcacacaa aaatggcata caaaaggtgt agacattgcc tcagatcatt
     3601 acaagcaatt tagtattcaa gatccagtgg aagatcgttt gacgtataaa gaaggttctg
     3661 cgcgagtgat agatatgagt acaggaaaag atattacttc agaaggtaca ctaacctatg
     3721 atagcaattc aagaacgctg aagtgggaag cttccgctga tttcttaagt aaaaatcttt
     3781 tagatggacg agaaattcag ctgatattta cagctaaaac tcctttgaat gaagaattgg
     3841 atatcgacaa ccaagcaaaa gtttcagtag aaagtattga gaaagcaact aatgtagtaa
     3901 cgattggggt aaaacctaac ttacctcaga tcattgtgcc aaaaactgga tcgaatcatt
     3961 tagtgctaac tgttattgga agtatattat ttcttacttt agcatcattg ggctatgtag
     4021 ttttaaaact caaataaata taaaaacaag gctgagaatc agccttgttt tcttttattt
     4081 gaagattgga ttcgcactaa taaatacatt atctacgcca attctaccag cagagtcaac
     4141 tacaaaatta tgacctaatc cgagaatact ttggttatta taaagtaaat ttgcattatg
     4201 ggcagggcta tttttccatc ctaccatggc atcgatagca ccatcgtctg ataaaaggtc
     4261 atcaatatac agcccagttt gacttatgtt ttctccctta gcaccgtagc agcctaagct
     4321 ttccaataaa tcttggaaag taccaccacc agggcgatag tgactgaagc tgacttgtaa
     4381 ttctttagct cgaatatctg cagcctgttg aacaatatca ttgaattgca ttggggcaac
     4441 accattttgt tggcgataat catttaacca agcaagttct ttttgacgca taaagttacg
     4501 taattcgtct aatgaaatct ttttatattt gaatgtgatt gttttatttt gatcgacgtt
     4561 attaatggat cgttggccgt ttttatccac ggtaacggta ggatcgtcat ttctgcacaa
     4621 gaaataattt tgatcagtca tgtttttcca aggagtagca aagctatccc cttcgtatac
     4681 tgtttttgat tcactagaga gaacagcgtt ggtatcagca tttacatgat taacagtcac
     4741 attaaattta cgagtgtagt tgaaaatata attttgactt tcagtcacat tgtttaaggt
     4801 tactgaattt gttggttcta cccgatcgtc taaaacatat ccttgaatag aaggagcagt
     4861 taaagtgaca ctgtcaccgt gagtgacatt gactatctgt tctttcaaaa tattaactcg
     4921 tccttcgttt ttgtagttgt tattgcacaa caaattgatt tgatgcactt gattataatt
     4981 gaatgtgatt gttgtattac ctgtgattgt tttgctttga gtatcattac caatcacttt
     5041 gtagctaaaa ttatctttag cgccttcgct atatttaaat gcttttgcct tagcagtgta
     5101 agtagaacca gaagttactt ttgcttgatc tgtagcaatc acttgacctg tttgattgtc
     5161 aatatggtta atagtaaccg tatattcttt tggtgtttca ggtttgtgtt gcaacccttt
     5221 aaatgcagca tcaagactat ttttagcatt gtttacttgt gtttgagaaa ccttagaatc
     5281 gtctagtact tttttagcat tggataaagc agtctgaaaa ttattccaag attcttctgt
     5341 aaaatctcct ttagcagttg attttactcg gttgtataaa gcttgtaatt cagctttatt
     5401 cacagtagga actggttttt tctttaatcc tttgtaagca gaatctaatt tatttttggc
     5461 actatccact tgcttttgag tcgcttttga gtcatttaac actttcttag cattgtttaa
     5521 agcggtttgg aatgcgttcc aggtattgtc ggtatagtca ccttttttcg ttcccttaac
     5581 gctcgtataa agagcttcta gagccgattt attcactttg ggtttagagg tgtcagcgcc
     5641 ataccaaccg ataccttcgt atttccagcc atagtctttg gttaaccatt gtacttcttt
     5701 ccaacttgca gtgtagtgat gtgagccaga ttttacacca ggaaggaaaa ggcggtagat
     5761 cggtttatta tttttttcgg cagaacgcca ggcaggccct tcataagtcc agccgtaatt
     5821 tttagtcaac catttcactt catcccaact agctgtgtaa tggtgatcac gaagaccttt
     5881 attgtacaaa cgatagacaa gatggccacc gctagtcggt gcttcccagg cggggccttc
     5941 ataatttccc cattttatct ttactagatg gtcacgttct tttttgctag aggtgtaaaa
     6001 atgttcatta ttatttggat tgtaaaggcg atacatgacg ttagttgaag cagcgtcaac
     6061 agatccagca agactaagtg aggcagcaaa tgttccaata gctaaaacag tttttggatt
     6121 aaataatttc attttttcaa ctcctaaatt taattaaaaa tgtttacatg attattatat
     6181 atcggctaac cgatatagtc aataaaaaat aagaaaagcc ttatataatt ttggcttttc
     6241 ttattttatt tgttagttga agtattaata tcaacgattt cttcagccaa tttatttaat
     6301 ttcatagcat ttatgaatct catgttgtct atactagagc gtttgttggc aaggtcactt
     6361 actgtagtta agggtacgcc agattctatg gatatagaac gaactgattt accactatca
     6421 aacagcaacc attcgatttt ctttttatca gcgtacattt caatgctcct tcttgttttt
     6481 tctttattat agctcttttt tgtaatgaat cacaatatca agagaaaaat tttctataga
     6541 aaggtggatt tttatgagta ttcgttctcc gtgtatccaa atgaggtttg ttgctacttt
     6601 cgttatttga tatactaaaa ataaacaacg aaagaaggaa taaaatgaag aaaaagcaac
     6661 tatggtttgt ctctatgttt gcaatatttt ttattatttt aagtggatgt tcaagtaata
     6721 agaaatcgga agctaaagaa gataatagtt tattgcaacc aggaactgtt tggaaagaag
     6781 aagtaggtgg tcttattcat aatttaaaaa ttatagatga tactacctgg gaatattctg
     6841 aaagtgtatg gcatccagat cctgtacaaa taacagttaa gaggcaaaaa gactatgaag
     6901 gtttagaaag atacaaaata gtagattctg caggcattag ggaatttata aacaaacctg
     6961 attcattatt tattgttgtt ccctatgaga aggatggcgt aaaaaaaata atttttcttg
     7021 gatctagcaa ggatgagaag cgaacgaaag agaaattaat tcatgatgga tcagagttaa
     7081 ataaatacaa attacaaaag actagtgagt gattgctagt ctttttattt atcctgaagg
     7141 aggaatcaca gtggcaaaag ttaaaacacc attcgaaaaa gccgcgtcta aaatcttaga
     7201 acgccgtggt gtttctgaaa tacagtggaa aaaagaagtt cttgaaaaag cacaatttga
     7261 cctattacga ggaaaagata aggacttaga agaatatatc tatcaacgtg aacgtgaaaa
     7321 acttgtattc gatgaaatta ttaaacatta aaaggagcga ataatcttat gtttgaatca
     7381 attacaatga attttttaac tcaaccgaaa gtagtagctg cagttgatat ttttagagca
     7441 ttacaaactg ccgcaactag tttgaaaaat aatctgatga tttttgctgc tgttgcggca
     7501 gtggcttcca ttgctcttgg aggtattttc ttcttctttg ggcgaagagg agctgaaaac
     7561 gggaagacac acgtaggtta tgtattgttg ggggtaggag ttattttagc tgccgcttct
     7621 attgtagttt caatgttctc aatgtttgga caaaccccac aaggactata aaaaaaacaa
     7681 gtaggaggag taaaaagtac gtggctactt tttactcctt tttttatgaa aggagaatat
     7741 tcatgttttt tacgatttca ggaaatgcaa aattggataa agagttaata agaaaagcaa
     7801 cgaagaaagt gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg
     7861 aaacggaaga acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg
     7921 ttaaattgac tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat
     7981 acgtttttgg cccactggat tatcaaattt cagaaggttt ttatcctaca ttgatagatt
     8041 taatccgcct tgatgtgttt aaagatacgg atgaaactat gagtgtggaa gagtcttatc
     8101 gaaacgaaaa acttttacaa gttttagaag aagagttaga gagcgaccaa agaaaagtga
     8161 atcaagtgag tgaagtcgaa gtaaatgaag aagtaccatg ggaacgacca atctttccaa
     8221 agaaggagtc aaaagaaaaa gagacagtct atgttgacga acaagctgat attccgactc
     8281 agaaatcgat atttgaggaa gagaacgatg tgatgggtca attggaggta gatccctttg
     8341 aagaaacaat ggaggatgaa gagacggacg aagtagaagt tgatccagtt gaagctaatg
     8401 aagagtcaac atggactgaa ggggattttc aggaatatat tgaaccactt atcagtgaag
     8461 aagtagaaaa aaacgaaa
//
LOCUS       DACBZN010000110         8272 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.110, whole genome shotgun sequence.
ACCESSION   DACBZN010000110 DACBZN010000000
VERSION     DACBZN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.110"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            7..624
                     /locus_tag="IVD64_002409"
     CDS             7..624
                     /locus_tag="IVD64_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149625.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IVD64_002410"
     CDS             1644..2813
                     /locus_tag="IVD64_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6149626.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="IVD64_002411"
     CDS             3286..3840
                     /locus_tag="IVD64_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6149627.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="IVD64_002412"
     CDS             complement(4013..4486)
                     /locus_tag="IVD64_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6149628.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="IVD64_002413"
     CDS             4498..5034
                     /locus_tag="IVD64_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6149629.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="IVD64_002414"
     CDS             5074..5445
                     /locus_tag="IVD64_002414"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6149630.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="IVD64_002415"
     CDS             5729..7000
                     /locus_tag="IVD64_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP6149631.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="IVD64_002416"
     CDS             7245..8270
                     /locus_tag="IVD64_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6149632.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
BASE COUNT         2944 a         1173 c         1456 g         2699 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aa
//
LOCUS       DACBZN010000111         8072 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.111, whole genome shotgun sequence.
ACCESSION   DACBZN010000111 DACBZN010000000
VERSION     DACBZN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.111"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(83..1114)
                     /locus_tag="IVD64_002417"
     CDS             complement(83..1114)
                     /locus_tag="IVD64_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149633.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1121..1951)
                     /locus_tag="IVD64_002418"
     CDS             complement(1121..1951)
                     /locus_tag="IVD64_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6149634.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(1948..2754)
                     /locus_tag="IVD64_002419"
     CDS             complement(1948..2754)
                     /locus_tag="IVD64_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6149635.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(2760..3584)
                     /locus_tag="IVD64_002420"
     CDS             complement(2760..3584)
                     /locus_tag="IVD64_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP6149636.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(3574..4674)
                     /locus_tag="IVD64_002421"
     CDS             complement(3574..4674)
                     /locus_tag="IVD64_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6149637.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4852..5637
                     /locus_tag="IVD64_002422"
     CDS             4852..5637
                     /locus_tag="IVD64_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP6149638.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            5670..6053
                     /locus_tag="IVD64_002423"
     CDS             5670..6053
                     /locus_tag="IVD64_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6149639.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            6129..6260
                     /locus_tag="IVD64_002424"
     CDS             6129..6260
                     /locus_tag="IVD64_002424"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6149640.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6229..6465
                     /locus_tag="IVD64_002425"
     CDS             6229..6465
                     /locus_tag="IVD64_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6149641.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(6543..7514)
                     /locus_tag="IVD64_002426"
     CDS             complement(6543..7514)
                     /locus_tag="IVD64_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP6149642.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2345 a         1895 c         1574 g         2258 t
ORIGIN      
        1 aaatcaattc taccttagat gttcttatat cactctatca gtcctatttg acaaagagcc
       61 agaaatgttt gccgactata cactagcttt gttgtgcaaa atgctgagta tcaatttcca
      121 cagggattgc tagaatatct ttcagctgaa tgtataaggc atcgctaatt ttctcttccc
      181 gatcgttcaa ccgatccact aaaatttctt tgccctccag cgtttgaaaa acataccgtt
      241 gaatgttgcc taaaataaat gattccttcc atagaaccgg aatcttaacc ccctgaggaa
      301 ccggttcaaa ttgaatcagc tccggtctta aataataaat gcagcgattg cccttagcgg
      361 aaaactcgtg ggtatactga ttcagctcat accattcgaa acgattatag ttgccgataa
      421 attccgcaac aaatcggctc tgaggattct gatacaattc cttcgaggtg gaaacctgag
      481 ccacccgccc agcttccatc acaaaaatcc gatcactgat tcgcatcgcc tcttcttggt
      541 catgagtcac aaaaatcatc gtcatcccta gcgattgctg gatagcgcgc aaatcccgct
      601 gcagctgttt tctaattcgg gcatccaaag cacttaatgg ctcatccagc agcaggactt
      661 tcggttcagt gaccaatgag cgagccagtg ccacccgttg tcgttgaccg ccagaaagaa
      721 actggacatt tttatctgcc tgctccgtca tatccaccaa tgatagcatc tcaaaaacct
      781 tttcctgaat cacctcagga ctcagcttct ttaccttcaa accaaaggct acattttcaa
      841 aaacagtcat agttggaaac aatgcatact gttgaaaaac aaagccgata ttgcgatcct
      901 tcgtaggctt atcggcaata ttctcattgt ccagcacaat ctcaccgctc tttggttgtt
      961 ccaatcccgt gatacagcgc agcaacgtag actttccgca cccgctggga cctagtaaag
     1021 taaccagctc acccttttca atctgaattt catcaatagc taaaatctgt ttttcatcaa
     1081 aaaacagatt gagatcatgt atttctaaaa acattttccg tcacaactcc tttaagggct
     1141 tctttttcga ctgcttctct tccctcgtct gcttaaggga cagctgttgc ttttgtcgat
     1201 aagtcagcat aaaggtagtt aaagcaattc ccaccataaa cagcaaatac aaaaccatca
     1261 cagcgctagc caaatggccg ttttcgttca tccgccgcag catatagatg cgtaccgtct
     1321 gaaatcgacc gccaatcaac aggttcgtca gcatgtactc cccaaaaacc gaagaaaaaa
     1381 tcatcagaga aactagggtc acaccgatac ggatgtttgg aaacagcacc tggataataa
     1441 tcgtagacat cggggcgccc aaggtttctg ctgcatccac taattccttc agattaatta
     1501 agcgcatctg gttgttaaga ctaagataga caatcggcaa cgccgtaata aacaatgcac
     1561 ctaccaacac tacaaacatt gggataccag tttcagcata cgttcgaagc aaagccgtta
     1621 ccaaaatcac tcctggtaaa gcatacggca gcaggagcag cttttccagc caacgattca
     1681 aacgagggaa atacaaatga atccaaataa tggtgggaat catcaacaac agaatcgtca
     1741 ccaagaccac acctgctaat aaaagtgagc gacccaccgc agcaataaat tccctgtccg
     1801 tcagcagctg ttggaaccat tgcaaggtaa aatcactagg taaaagcgat tttgaccatt
     1861 tagtggagct ggcatacaaa atcgttccaa taatcggcag caagtaatac aggacaaagc
     1921 agaacaaaat cggttttatt aactttttca cgtctgcacc accttgcctt tcttttgaat
     1981 aatcaacatt gccgttacca gcaggacacc taaagcgact gccaacgcac tgcctaaatt
     2041 aggcttggcg aacacatctc ccgacactaa agccgcaatt cgaatcgtta aaagatttac
     2101 attactgctg gtcagggcat acgctgtttc ataggtcccc atgccattag caaataagat
     2161 gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa gccaccttga accaagcata
     2221 cgctgatgaa gcgcccagca attgtgcgga ctccagccat tcctttttca cttccttcat
     2281 gctgggatac aaaaacaaga cccctaacgg aatttggaaa taggtatacg tgatggtcaa
     2341 tccccaccaa gaatacaaat tgaagtccgc tagaaatggc aaaatcattt tcagcacacc
     2401 tacattcccc aacaaaataa taaaagagaa tgccagcgga atcccagcaa aattagctgc
     2461 caaattggaa acaaaggtga ttttctcctg caatttagtc gatagattca ttaagcataa
     2521 acataaaata aacgtcccga ttaacccaat cacactggag ctgacagcaa tcagtacgct
     2581 gttaacaaag gcctgtgtgt aatatttgtt gctgaaaata tcactgtaat tggccaatgt
     2641 ccactggtta gttaaatcac tgtgcaagct gcacagaacc ataaaaagca gcggcagcag
     2701 taaaaatgca cagaagataa aggaggcagg aaccagaacc aagcgttttt tcatctcttt
     2761 catcccctca tcaattcttg aatgtcctgc attttctctg acggtgcaat tcctaataac
     2821 cagcaaaaca atggagccgc cagcagctgt ggtacagttt gttccttcag cttttggcgt
     2881 ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag ccaagctgcc gccatgcagt
     2941 ccgtgcgcat ccatcccgtg atccgcagtc accacgactt gataaccttt ttgcagccat
     3001 tcaggaaggt aaatagccaa taatctgtcc gcctgattaa ccgctgctgc atattcatga
     3061 gaatcggcgg taaatttgtg accgacatca tcaatattca tcgaatgtac gaccaaaaag
     3121 tctggctgat actgattcac taaataatgg gcatccgcaa ataaatgcga atcagggtaa
     3181 gtgtcctcac tataaaagat gccatgctga atcagtcgct cctcctgatg ctggatgcga
     3241 tcaaccaaac gattataggg ggcttgattg tacaactcgc tgtaccagtg gtaagcggcg
     3301 gctcctgttt ttccgccagc ttccttaact aattcaaaca gcgaccactc tttggatcgc
     3361 tgagcatggt aattactata gatgccattt tcataggctg gaacgccggt catcagtact
     3421 tcgtataacg gacgtgaatt gctgggcagc tcacttttta ccttcaataa gcttgcttgc
     3481 tggtgctcaa ctaaatgatt catgaaccca agctgggcga cggctgtgtc gtagcgacag
     3541 ccgtctagta taactacaat cagtttgtca agtctacttt gcataggtca gtacctccgc
     3601 ctgccaaagc tctggaagct ctacagaggt cttgttccaa gcttcattgt cttcaactgg
     3661 caccgccgct tcataagcat cctccggcag taatttttca gctacttctt taggcagctc
     3721 taccttgcga ataggtcgcg catagccctt tgccaaattg atttgccctt catcagataa
     3781 aatatattcc cgtgctagct ttgccgcatt gggatgaggg gcatttttat tgattaaggt
     3841 tgtatacccg ctaatgatgg agccgtctgt tggcacagtt acctcaaaac gctcggagtc
     3901 aatttggtca cgataattta gtccattgaa gtcccaaaca actgccacat cgatttcacc
     3961 tttttcaaga tttgctacgc tggcatcaac ggtggacaaa cggccttttt ctgccatttt
     4021 tttgtagtaa ttcagacctg gctgaatatc cttttcgtca cctccgttgg caatcgccgc
     4081 agccaataca gcaaattgtg cttggttggc aatcgtcaca tcccccacgc taacttgata
     4141 gtcaccctct tgcagctcct tccagctagt cggtgcttgc ttcacattct ctttatcggt
     4201 gataaaggaa atggttcccg tatagcttaa caaccaatcc ccatcatcat cctttgccca
     4261 atccggaatt tcctcccaat agttggtctt ataaggtaag gttaactctt tgctttcagc
     4321 caacggaccg aagctgatac cgacatcacc aatatcggcg gttccatttt ttccttctga
     4381 ttcaaactta gccaattctt ctgcactgga catatccgta tccacgtgac ttaagccgta
     4441 ttctttttca aggtcgttcc aagtgcctac ccaatttgcc caagtatccg gcattcccaa
     4501 gctggcaatc tctccttctt ccttggcttt ttctataatc gtatctaaag agtcgtctgc
     4561 cgcagccgat tgcttcgttg cttcacctga gccgcagcct gtcagcagga tacttactgt
     4621 tgctgcacaa aataaaccga tagtcatgtt acgaattttt tcattcattt tcattcattc
     4681 ttctcccctt tcgataattc cattctaaca aagctttgta atgagagaat cagtttcttg
     4741 aaaaggtttc atgaacaatg taaaggttgt atatggtttg taaatttcga cgaattcccg
     4801 ttaaccttct aaagcttttg cggtatgata gtagaaaaaa ggaggctccc tatgcagtat
     4861 tatgatcagc atctccacac gtatttttcc tttgattcag aggaaaaatt tgaaaactat
     4921 ttagcctatc agccggagtt tttcgtttct acggatcact ttgatttaaa caatccatct
     4981 accggaaaag atgacattcc ggattatcca gcctatataa acaaactgga ctctttaaaa
     5041 gaaacctatt ccaccaaatt tctgcgaggt atcgagattg gcgttgttcc aggacaagaa
     5101 gagcggatcg tggattacct cgctcagcat ccttatgatt taaagctggt aagtattcat
     5161 caaaatggcc gctttgatta tatggacgat gtggttttaa ccaaagatcc cattcaaact
     5221 accaaggaat attttgatca gatgttgcag gtcttgacgg attttccaga aggagatatt
     5281 ttgactcact ttgattatgg attacgccgc ttcaccttca cagcggaaga attcgcggag
     5341 cattttgagg ctcagctgct aacgattttc aaaaaggttg ttgaacgaga attggcgatg
     5401 gagttaaatg ccaagagctt cttgaaatac ggcaacgaag acctttaccg ttatgccgtt
     5461 cctttgtatc aaagtgtcgg cggaaagctc tttaccttag gctcggatgc tcatgttgcc
     5521 gaagattatc agctgggctt taaaaaaatg agtcagctgt tggcagactg tcaggtagaa
     5581 aagttgctag tcattcaagg aaaagaacgc cgcctgacgc cgctgccgat tttgtgattc
     5641 ctatataata gaagaaagtg gtgaaaaaaa tgagcaacct gtgggactac aatcaagagg
     5701 cacccatcca ttatttaatt gcgcgacatt gggatgcgtt gaaaattgag gcggtttgcc
     5761 gatccttgtt agcagccgtt ccaaaacaac aactggaaaa ttttttggtg gctgacagct
     5821 tgcagcggga aaaagttcag gcttattttg cagcctttaa agatcagcct ttggaatatt
     5881 tgcatgcgca gtttcatctt ttttatcaag tagccgcccc agatgactac aacgatttac
     5941 gaggacagct gcaattgacc tttcaagcgg atgaaaccgc ctataccgtt ttattgggaa
     6001 tggctcgcct tggtgatcag gcgaaggtcg agtggcggat tttcgatatt tgatggcagg
     6061 atcaaggcgt ttcagttgca aaccttcctc tctttcaatt agaaaaaccg cagcaggaag
     6121 aagtttgtgt gctggtcacc gatgctggtg aaattcgtct ccgcttattt cctgaccaag
     6181 ctcccctggc tgtagccaac tggcgctccc tagctgagca aggcgtttat gacaacacac
     6241 ctttcggctc gtgtcattaa ggaatttgtt attcaaggtg gcgctttgga tggttctggt
     6301 gatgaagctg tttccagcta tgagggcttt ttcgctgatg aagtgcatcg cggactttat
     6361 cattttaatg gcgccttggc cttaggggac cacggccccc acaccaacgg caaccagttt
     6421 tttattgtgc aaaacacaaa ggcgcaggct gacctgctaa tgtaaagcaa gcatacttgg
     6481 agcttggagg actaccggag ctggatggtc gttatactgt ttttggacag gtataccaag
     6541 ggttagaaat cgtagcagcg atcgcagctc agccgacaaa cagtgaagat gtaccgattg
     6601 agccagtgtt cattcgacaa attaacttcg aaaaaacatc gtaaaaagac actcactgac
     6661 ttcagcaaaa gctctattct tgagctggct gcagctagtg agtgtctttc atttatccat
     6721 aagataaagc ttggattcaa taaataaata aatgttttgg ttaaagctgt gaccagcaaa
     6781 tgaggaaccg tgcgagccag ttgatctgcc aatgcaaaca tttctttagg tgaaggcgct
     6841 ggcgtgtctg ccaacggata ctccagtccc aatgcttggt aaccgtgcat accatagcag
     6901 tggtacggta gtaattctaa cggtacgaac cgatcaagat ctgccacaaa acaagcaatc
     6961 gtcagcagct cggtcaggtt catattgata cctggaacca caggaactcg aattcgcact
     7021 tcgcagttag gatactcaat taaaaagcga cgaatattct tcaaaatttg ttggttatct
     7081 aagccggtcc attgtttgtg gagcttgctg gtaaacattt ttacatccac aaacaatgta
     7141 tccacatagg gagctacctt ttggatttca ttataagcac cacaaaagct ggtttccagt
     7201 acggtattga ttccaatgta tttcgatttt tgcaaaattt ctttagcaaa atccgcctga
     7261 accagtgcct cgcctccgct aatggtcact ccgccatccg agtgaaagta aaaaacagca
     7321 tccttctcaa tctcatccat tacctcttca gcggtcatgg tctggccaaa tcctttttca
     7381 atctgcttgc tttgtgattc gggaatcgaa caccaggcac agcgtaatgg gcagcccttt
     7441 aaataaacaa cggtccgtaa accagcacca tcatgtagag aaccctgttc tattcttaat
     7501 actgttcctt ccatatagat ttttgaatta cacactagtt taaactaaaa cacacaagga
     7561 aaaacttacc atttaatcat aaaattagct catataaata tactaggatg taaaatagac
     7621 tggtcaaaat ctggtcaaaa aagaggacta atacaggtag tcctcttttt tgttatttta
     7681 atattctaaa tcatttttta ttggtttatt cccttattcg atgtagactc aaatcactat
     7741 atcactagat atcaaacggc gtcatacgga atcaaaaaca tcaaatttga cctgttaaat
     7801 ggtcattgaa taacattcaa aatcaataaa acggagtcag acacggagtc aaaaacgctg
     7861 agagtaaaaa atatatacag ccttgtaggt aaagtaccac agggctgtat attttttatt
     7921 tcagattacc ttaaatgatt ccatttactt tttatctcat tttttcctac tagttagtat
     7981 caaatactct cctattttct caataaacta tttttggata catggtatct gaaacagcta
     8041 aaaccttgtc attacagcaa aaaatcacgt ta
//
LOCUS       DACBZN010000112         8061 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.112, whole genome shotgun sequence.
ACCESSION   DACBZN010000112 DACBZN010000000
VERSION     DACBZN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.112"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IVD64_002427"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IVD64_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP6149643.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="IVD64_002428"
     CDS             complement(2944..3378)
                     /locus_tag="IVD64_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149644.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="IVD64_002429"
     CDS             complement(3395..4600)
                     /locus_tag="IVD64_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP6149645.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="IVD64_002430"
     CDS             5294..5680
                     /locus_tag="IVD64_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6149646.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="IVD64_002431"
     CDS             5774..5965
                     /locus_tag="IVD64_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6149647.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="IVD64_002432"
     CDS             6479..6811
                     /locus_tag="IVD64_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6149648.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="IVD64_002433"
     CDS             7107..7262
                     /locus_tag="IVD64_002433"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6149649.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8061
                     /locus_tag="IVD64_002434"
                     /pseudo
     CDS             7538..8061
                     /locus_tag="IVD64_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2665 a         1443 c         1185 g         2768 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat aaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac aaaaaccatt caaagtagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tacctttcat gaggatgcct ttcgcaaaga
     7621 gcgcatcaat gacgaaacat gttttgcttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa a
//
LOCUS       DACBZN010000113         7769 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.113, whole genome shotgun sequence.
ACCESSION   DACBZN010000113 DACBZN010000000
VERSION     DACBZN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.113"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            544..1107
                     /locus_tag="IVD64_002435"
     CDS             544..1107
                     /locus_tag="IVD64_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6149650.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IVD64_002436"
     CDS             complement(1386..1673)
                     /locus_tag="IVD64_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6149651.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IVD64_002437"
     CDS             complement(1663..1992)
                     /locus_tag="IVD64_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6149652.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IVD64_002438"
     CDS             2297..2626
                     /locus_tag="IVD64_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6149653.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IVD64_002439"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IVD64_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6149654.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IVD64_002440"
     CDS             complement(3551..4462)
                     /locus_tag="IVD64_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAP6149655.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="IVD64_002441"
     CDS             complement(4890..5498)
                     /locus_tag="IVD64_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6149656.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IVD64_002442"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IVD64_002442"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6149657.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="IVD64_002443"
     CDS             complement(6528..7496)
                     /locus_tag="IVD64_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6149658.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2372 a         1543 c         1292 g         2562 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatta tggctctata ctttttgatg ttggtggatg acaccagcat
     7741 cttttttatt ttccaaaaca tatagaaaa
//
LOCUS       DACBZN010000114         7731 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.114, whole genome shotgun sequence.
ACCESSION   DACBZN010000114 DACBZN010000000
VERSION     DACBZN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.114"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(39..545)
                     /locus_tag="IVD64_002444"
     CDS             complement(39..545)
                     /locus_tag="IVD64_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6149659.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            705..1145
                     /locus_tag="IVD64_002445"
     CDS             705..1145
                     /locus_tag="IVD64_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6149660.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(1171..2328)
                     /locus_tag="IVD64_002446"
     CDS             complement(1171..2328)
                     /locus_tag="IVD64_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149661.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2331..2687)
                     /locus_tag="IVD64_002447"
     CDS             complement(2331..2687)
                     /locus_tag="IVD64_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149662.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(2985..3959)
                     /gene="sgrA"
                     /locus_tag="IVD64_002448"
     CDS             complement(2985..3959)
                     /gene="sgrA"
                     /locus_tag="IVD64_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP6149663.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(4155..4994)
                     /locus_tag="IVD64_002449"
     CDS             complement(4155..4994)
                     /locus_tag="IVD64_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6149664.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            5179..6066
                     /locus_tag="IVD64_002450"
     CDS             5179..6066
                     /locus_tag="IVD64_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP6149665.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(6650..7345)
                     /locus_tag="IVD64_002451"
     CDS             complement(6650..7345)
                     /locus_tag="IVD64_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP6149666.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(7329..7727)
                     /locus_tag="IVD64_002452"
     CDS             complement(7329..7727)
                     /locus_tag="IVD64_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6149667.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2417 a         1532 c         1137 g         2645 t
ORIGIN      
        1 ccagtatcca ttctgctttt tttaaacgaa gaaacaactt attgctccaa aaatgtcaga
       61 aatcgtttat cccaaatttt ctcgtaaaaa gcgattgttt cctttgcttt cataaaagga
      121 ttatcataag ttgtcgttgc tcctctacac atcataagct ggtatcctaa tccatgagca
      181 aacttcacag tcgtatctat acaatactct gcttgtgccc cacataattc gatcgaatga
      241 cagtccaatt cagttaataa aacactcaaa ttagtcttat aaaatgaatt cgcatgggtt
      301 tttctcacaa aataactgtg ctcaggaata gataattccg acaatagctc ccatggtttt
      361 gtttgtggca ctaattgagc atcttcgtgt tgaacgaaaa taactggttt cccaatcgtt
      421 tcgtatgttt tgattctttt attcactaat gctgtcactg tttctaattc gtgtatcggc
      481 gaagaatcaa aacatacccc tttttgtaaa tcgatcacta ctattgcgtc ggctgtgttt
      541 gacatacttg tgccttcttt cttttttctt gatttaatag tatttcttat ctagctagtt
      601 ccattatacg tgacaacatt ttcttatttt acacctctaa atggcacttt tcgactattt
      661 cttcatgtta ttatttcata aatacttagc tattggagga atgtatgatg attaaattgt
      721 atacaggttc tggctgctcg tcttcaagac gcgcaaaacg ttggcttatc gtccatgata
      781 taccgtttat cgaacagaac ttaattcgtc aaccactaac caaaaatgaa tttatccact
      841 tactaaaatt gacaaacaac ggcgtgaccg aaatgctctc tactcattct attagttata
      901 aaaatcttgt tcacagaata gatgaactca gtttgagtga actatatatt gagattcgta
      961 catccccttc tttattgaaa aaaccgatta tctacgacca atctgcaaaa cttttgactg
     1021 gatttagcga agataaaatc aggatgttcc tttctaaaac taaaagaaag caagagctca
     1081 acgaactctt gctttctggt catcttgtcg attttaaaga aaataatctt tttgcaatgt
     1141 cttaattttg gcataaacaa tctgcttttc ttaattaacg aaatccaatt tttccaatat
     1201 ggatctattt ttctcattcc tgatttttat gatttcagct ttcagacgcg cccaatcatt
     1261 ttttgtaggt tgtccattcc agtaaaatat ctgtgtattc gtttgatcat atgccttttt
     1321 gtcgatagga taatcgctaa tgacaatatc aggtaactga tcagcggatg cactatattt
     1381 tattgggcgt tcagtcatag ataatatcct tttctttgcc cataattcct gtgttttacc
     1441 atactttgat tgaatataga atatgacagg ttgttcgtgt tttataaaaa tatccctgac
     1501 taacatacag tattgaaaaa tcaaccgtct gttcttttca taataagtat taagctctgg
     1561 aattctgcga gctaccccac taaaaaactg ttgcatgatt ggatacatca ctggaaatat
     1621 atgctgaaaa tctctgtctt ctgctgattt tccaaaagca tccaccttct tttttgtccc
     1681 aaaaaccctt gaatacaaat ggatcattgc caaattgaca tatagataat ttttttctga
     1741 agttgtaaaa caaatcttga agtttttttc aaaattttct acaatagacg attcaatcca
     1801 gccccaactt tcttgtactt tatctggagt atccaacaaa tcttcttcca aataggtgtt
     1861 cattacacca aacataaaat aaaggtaagc ttgctctgtt ggagtgatgt gattcatcga
     1921 ccagaagttt gcttcaaaaa aaggctctag tatgtaatgc gtaaactctt cttttgttat
     1981 caatggatgc acaaatttgt tcatctcttc tgggacatac gtaatcgagt ggccttgttt
     2041 cattcgttgc aacgaaattc ttagtaggct gatccatttc tttctatcta tactacgcgt
     2101 atatggcgca tgtttcgcaa atttttctaa tagttgacgt ttatttttta tacttgataa
     2161 tatatcagaa tgattttcgt cgaatgactg ccaatacatc aagtgaaaaa agtatcggat
     2221 acggtattct tctccttcta tcggattacc ttttttcaag ctgcaagata aatgatagtt
     2281 tttcaaaata gtattaagtt ctcttaattt tctttgaatc gtacgcaaac ttatataatt
     2341 ttttgactaa gattttctaa ggattgataa gtacctttgt aaagtgaatc cagtaaaata
     2401 taaccaatcg ttccctctaa aagtatagaa taaactttac tcagtaaaaa ctgagaagaa
     2461 aactcgactt ttatcatctt gttttctgaa agaaaagata atttatattc tggatacctt
     2521 tcgcaaaacg attctgcttg ttgaatcacc gaattcatct tagccagggt gtagtttaga
     2581 tctaaagcga tatccttcac cacatataat ttctcgtctc tttcataaca atagttcaaa
     2641 aattgatata cttgcctttc attgatatca ataaattctt caaacaaagt acatactcct
     2701 ttttatctat tatttagact tattccgaca gctacatgaa taacgtacaa atagattcgt
     2761 tactgatttt tcagagatac taccatgcgc agtgtgcatt gtcaattatt cttgctctta
     2821 ttttccattt gtgttcctgt tatttcctgt ttctcaaata tgttagtaaa caaaaataga
     2881 tctggcaaag ctacaacttc gccaggcaat acagcactct cgaatgaaat attcactcaa
     2941 aaatctatcg ttttattcaa ttttagatct gttgctttta ttctttattt cactcgatgg
     3001 aagagagaac gaaaatagct tgctcctaca atcaaaatga gtaacacacc catccatgta
     3061 attagcttgt tcaaggttct actaccagtt tctggtaaat cgctttttct attatctgtt
     3121 tctttcgctt tttcttgatg atcaattcca cgagaaacaa aatcaggctg ttttagcaca
     3181 ctttttaggt tgctccctgc atttctcttt tgttccttag ttggtatgat aggtaccaaa
     3241 tcaagttcat tatttacttt tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc
     3301 tcactactca tgcttcctgt gctactttca ctactcgtac tctccgtact gctttcgctg
     3361 ctcgtgcttc ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg
     3421 ttttctgtgc tactttcact gctcgtactc tctgtactgc tttcaccgct cgtgttttct
     3481 gtactgcttt cactacttgt gctatccgta ctgcttggcg gtccaacaac tgtccaactg
     3541 cttggtttca aattctccgt gatcgtcatc tgtccatcat aaaaaacaat caaatcaact
     3601 ttatctttac tcttgatctc atcaatcgat agttctgcag aaatcattat tgtatactca
     3661 ataccagcgg tccattgtac gtcttttaaa taaacttccc caataacttt ttctgaactt
     3721 ccattaccat cccaaccata tgttagaaca gcatgtacat cactagcatt cacgcttttg
     3781 tttggtttga ctggaatagt aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt
     3841 ttcacagtaa atgaaagatt ctcatttgcc cgttcatttg cagaagtact gattccaatc
     3901 attgctgcaa ttatactcag cccaagaata acaaataata cggtcgctgt tttcttcatt
     3961 tctatcactc tccatataat taccttctat ttgataaagg aattatataa aatgaaaatg
     4021 atgagaacac gacagtcagt tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat
     4081 ttgtttactt tttcgtttaa tcaatgacag acttaaaaaa gcaagaccga tatcagcctt
     4141 gctttcttca tcttttattg ataaacagca gtgtatttct tcatccgctt tgttttatat
     4201 gcatagagta acaaaccaac aatcaagact aacaagaata atgtagttga tatagatgct
     4261 tcagccccaa agtttccccc agtaattatt gtattttgcg attggatcag agtggagaaa
     4321 aaaccttgtt cttgcgttcc gctgactggt atacccaaaa tagctccttg tgtccaattc
     4381 caagtaatat gagcaccaat agccgtaaat aaacttcctg tactgtagat aagtgcgctg
     4441 aaaaagcaac ctgctaaaaa gatattgaaa actgccagaa atgtcatgtt aggattcaat
     4501 ccgtgcaaaa gagcaaataa tgcagcagat ccaatcattg ctattaacgg accaaatcta
     4561 agcatctgat gctgaaaaaa gccacggaat aaaagctctt ctgatgcccc cactgcaata
     4621 aataaaataa aactcgttat cagcggcaca atgtttgcgt ttcctgaatc taaccgatac
     4681 gcccccgtca aatataatgg actagtaata agcagaaaca ctccaattcc aacaacgaga
     4741 cctcccgcta aataccccca gtcttttcct cgaacagaaa caccataatc ttctattctt
     4801 tttccttcga ccttactagc aagaaataaa gctagtaaaa aaacagctaa atgtccgacc
     4861 gtaccggcaa ttgctccaat ccattcacta tttccaataa acatatccgc aagtgcatta
     4921 ttgacaagaa ttcctaaaac aaaaccaact aagactatta aaacagatcc aatcaaccga
     4981 ataatcctga tcatctttca tccctccttt atattaagtt taacaaccct tttctttctt
     5041 gtctcgtgaa aactatgtaa atgttctcca tcatagagag aatagatttt tacgaactaa
     5101 gaatatctac agcaacctta cgaaaatgaa agactgtctc cctaattctt ctgttagact
     5161 gtttttaata aggaaaggat gggaaatttt ttgaaaaaac aactactaat tatgtgcctt
     5221 tcagcatgtg caattttact attctcaggt tgtagcaagt ccaatgaaac gattgcaacg
     5281 tttaaaggtg gaacacttac agttcaggat ttttatgata acccaaaagt taaatcaaat
     5341 aatcaaacac tattaaaaaa aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa
     5401 gagatttctt caaaggaagt aacaaaagaa tataatgaac aaatcaaaaa actcggtcca
     5461 aactacaagg aacaattaaa agcagttggc caaacagaag agacatataa gaaactcttt
     5521 aaacagatgc tggctttcca atacggtttg aaagccaatg taaaattaac agataaagat
     5581 ctagacactg cttggaaaga attctatcca gaagttagta cacagatcat tctattttcg
     5641 actaaagaag aagcagataa agcaaaaaaa gaagcaaatg aaggggaaaa tttctccaag
     5701 cttgttcaag cttatggaaa aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt
     5761 gattcaacca atccagagat tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac
     5821 ggggaagttt ctgatatcat tcctgtaaca gatccaacga cttatcagca aagctattat
     5881 ctagtaaaaa tggtaaaaaa acaggataaa ggttctaata aagacaaata caaatctgag
     5941 ctagaaaaaa ttgctaccga agcaaaaact cttgatacgg aattcatgga taaaacaatc
     6001 cgtaaagtga tgaaaaaaga caatgtaact attaaagatc cttatgtaaa aaatatattc
     6061 aaataaacaa atttgaccca attggggatg tggcaaaatt tgagaagtga tttttggaaa
     6121 ttccttctta tttttgaggc aaaccacttt tgccacaact tcatcggggc ctttttttat
     6181 ttgtaccaaa acaaatcact ggatttgtta caatcatgag gatataattt aaaaaagttt
     6241 tttatttcta tcacatgaat caatgcgcca ttaagtagat tctttcatgc ttccctattt
     6301 tttcacttgc cgtatcgctt caaaaacaac tatcataaac tcaaactcga ctgaaaagtt
     6361 attttaaaaa agactaaaaa tatcccttct ggacttgttc ctcttattct gttatactag
     6421 agaaacacga aagatatttt tatgcgacac ttttcatttg tttttatgaa atttattcaa
     6481 aactggcgta acttcagagt tacaaggatg tattagaggt tagggaatac atcgtttaaa
     6541 tgagaaataa gtaatagtca cccaaacttt aaattaatga atattgaatc gtcagatagc
     6601 ctcctcgagt tttcgagaag gctatcgttt attttatgga gaaattgatt cagaagtaat
     6661 cagcgacctt ttttagttca ccaatcgttt ctgcttggaa agtttgcacc caatcttctt
     6721 ggaagcccct cactgcttta tcaatcgagg gcatctctaa tacttgatct agtagctctt
     6781 tacctaacgt tatcgcatga gctccagatc gaatggctgt agtgacttgt tcacaatttt
     6841 tgaaactagc tgctaatatt tttgttggtc tttgcgtacg ttcgatctca gtccgcaacg
     6901 aatcaatcac tttcatagca tcaatgcctt gattttgcat cctgttataa taaggtgcaa
     6961 tataatctgc tccattatta atagctaaat acccttgtaa ttctgtgtaa atagctgtac
     7021 cggtaatcaa ataaccttct tttttcaact ttttgatcgc ttgaaggcct gcttgattca
     7081 ccggcacctt gataaacgta tcttttccca gttcttctat gataactcga gcatcctcta
     7141 tcatttcttc ttccgttctt cctattgtct gaatatggat aggaatctgt ccgattattt
     7201 ttttgatttc tttcatatgc gagaagaagt ttgacgctcc ttcttttttc agaattgtag
     7261 gatttgttgt tacgcctgtt aaattcaaaa actgttgaca agaataaata ctttttgtat
     7321 ttgctgtatc aagcatgatt tccatatagc tttcccccat tctgatcctc ttgatcgatt
     7381 ttaatgattg tcccctttct taaatgcggc atttccttat cttctacata cattgttcct
     7441 ggaagttccg gctcatgaga tccatcaaat tttatcgtga tatgtcctaa ttcattcaaa
     7501 ttagtttgga caacatctcc tactgctgtg attacataca tttgtttatc aaaacagagc
     7561 ttcatatcac aagttatttc ttttcttacc tgattcaatt caatcttata acaataatct
     7621 gttaatgttt ccggtgcttc atctccaaat aaaatcatta ttttttcttt cctgaatact
     7681 tctgcttcta taccaacatc tgttacaacc gtttcaaaga tcgtcatctc t
//
LOCUS       DACBZN010000115         7597 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.115, whole genome shotgun sequence.
ACCESSION   DACBZN010000115 DACBZN010000000
VERSION     DACBZN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.115"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(136..426)
                     /locus_tag="IVD64_002453"
     CDS             complement(136..426)
                     /locus_tag="IVD64_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149668.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IVD64_002454"
     CDS             complement(544..804)
                     /locus_tag="IVD64_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149669.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IVD64_002455"
     CDS             complement(941..1354)
                     /locus_tag="IVD64_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP6149670.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IVD64_002456"
     CDS             complement(1810..2295)
                     /locus_tag="IVD64_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149671.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2679)
                     /locus_tag="IVD64_002457"
     CDS             complement(2434..2679)
                     /locus_tag="IVD64_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP6149672.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="IVD64_002458"
     CDS             2872..3672
                     /locus_tag="IVD64_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6149673.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="IVD64_002459"
     CDS             3691..5559
                     /locus_tag="IVD64_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6149674.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="IVD64_002460"
     CDS             5552..6043
                     /locus_tag="IVD64_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP6149675.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="IVD64_002461"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="IVD64_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP6149676.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="IVD64_002462"
     CDS             6603..>7597
                     /locus_tag="IVD64_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP6149677.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGALRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1443 g         2234 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac atgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggaggggct cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt tcccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACBZN010000116         7483 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.116, whole genome shotgun sequence.
ACCESSION   DACBZN010000116 DACBZN010000000
VERSION     DACBZN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.116"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            83..550
                     /locus_tag="IVD64_002463"
     CDS             83..550
                     /locus_tag="IVD64_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP6149678.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            557..3049
                     /locus_tag="IVD64_002464"
     CDS             557..3049
                     /locus_tag="IVD64_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP6149679.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            3270..4193
                     /locus_tag="IVD64_002465"
     CDS             3270..4193
                     /locus_tag="IVD64_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6149680.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            4211..5452
                     /locus_tag="IVD64_002466"
     CDS             4211..5452
                     /locus_tag="IVD64_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6149681.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            5586..6932
                     /gene="gor"
                     /locus_tag="IVD64_002467"
     CDS             5586..6932
                     /gene="gor"
                     /locus_tag="IVD64_002467"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP6149682.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         2599 a         1204 c         1629 g         2051 t
ORIGIN      
        1 tttaacaaaa agcgttcttc tttctttcta ttatcaaatt cgttataatt aaggtcaata
       61 ttagtcaaag ggaatgaata agatggcaca tcagaatacc tcggatttaa ttgaatcata
      121 tataaagaaa attctggaaa atagtgaaat gatcgagatt cgaaggattg aaatggcaga
      181 cctctttaac tgtgttcctt cacaaatcaa ctatgtgatt aatacccgtt ttaccatcca
      241 acgtggttat ctagtagaaa gcaaacgtgg tggtggtggt tatatacgaa ttgcaaaagt
      301 aaggatttca gataaaaaac aattacttga gcagataaat cagttatttg atgatacaat
      361 cagtgagaaa aatgcttttg ctattattca aaaactttat gaagaccaaa taattacaaa
      421 aaaagaagga aacttgatgt tgagtgcaat tgcaaaaaac acattgaatt tcaatgaata
      481 tgaagatcat acacgtgctc ggattctccg agcatttctg gaacgtttaa gttatgaaga
      541 tgggaagtga gattagatgg aagaactttt tactgaaaga gcccaagcag tattagaaat
      601 tgcacaagaa gaagcaaaac ggttgaaaca tcaatcagtt agttcagaac atgttttact
      661 agccttagtg gtcgagcaaa atggaattgc tggaaaagta ttacgtgaaa tggatttgaa
      721 tgaaacggaa atttatgaag aaattgaaca tctgattggt tatggtggta tccgttctta
      781 ccctaaagga gtctttcttc catattcacc aagaatgaaa caagtttttg ctttagctag
      841 cagcgaagtg aagaaaactg gttctcaaaa agttggaaca gaacatattt tgatgagctt
      901 attaaaagat gaaagtatca tggctactcg aatcatgatc aacttaggta tcagcctttc
      961 aaaagcccgt caagttctga aacaaaaaat ggggctagct ggtgattctg ctggaaaagg
     1021 aatgaatcgt cgtagaaatg taaatgtaca agataaacgg caaacaccgc aaggaacacc
     1081 tacgctagat tcattagcac gtgatttgac gaaattagca aaagaaaaca agctggaccc
     1141 agttgttggt cgttcgagag aagtgaaacg gcttatccag attcttagcc gacggacaaa
     1201 gaataaccct gtactggtgg gtgaacctgg tgtaggcaaa acagccattg ctgaagggtt
     1261 agctcaaaaa atcattttag gtgaagtgcc agaagacatg caagaaaagc gcctgatgat
     1321 gcttgatatg ggatctttgg ttgctggtac gaaatatcgc ggtgaatttg aagatcgaat
     1381 gaaaaaggtc atcgacgaaa tctataatga tggtcaagta atcttattca ttgatgaatt
     1441 gcatacctta attggtgcag gtggagcaga aggagcaatt gatgcttcga atatcttgaa
     1501 accggcgtta gctcgaggag aattacaaac gatcggagca acgaccctag atgaatatca
     1561 aaaatatatc gaaaaagatt ctgcattaga gcgacgtttt gctcgcattc aagtagatga
     1621 gccaactcct gaagaagcag aagtgattct tcaaggatta cgtacaagat atgaagagca
     1681 ccatggagtg gagatcacag atgaagctgt acgagcagca gtcaatcttt ctgttcgtta
     1741 tatcacttct cgtcaactac ccgataaagc aattgatttg atcgatgaat cagcagcgaa
     1801 agtccgtttg gatcaaaccg atcacttaac aaaatcaact gtgatcaaac tagaaatcga
     1861 tgaactagta caagaaaagg aagcagcgat ccaaaaacaa gactttgaaa atgctgctca
     1921 attacgcaga caagaaaaag cactacgtaa aaagctgcaa aaagtttctg caattgaagc
     1981 aaaacaggag caaggttatt ctgatcgtgt aacagaagaa gatgttgcta cagttgtatc
     2041 ggagtggact ggtgtccctc tacagcagtt ggagaaaaaa gaaagcgaac gcttgttaga
     2101 actggaagga cttctgcatg aacgtgtcgt tggtcaagat gaggctgtca aagcagtttc
     2161 acgtgcgatc cgccgtgcac gaagcggatt gaaagatcct gatcgtccaa ttggttcatt
     2221 catgttcctt ggaccaactg gtgtcggaaa aacagaattg gccaaagcac tttcagaagt
     2281 catgtttgga agtgaagatg ccctgatccg tgttgatatg tcagaattta tggaaaaata
     2341 cagtacaagc cgattaatcg ggtctcctcc aggatacgta gggtatgatg aaggtggaca
     2401 gttgacagaa aaaattcgtc aaaaaccata ttcagtgatt ctcttagatg aagtggagaa
     2461 agcacatcca gacgttttca atttacttct gcaagtattg gatgatggtc atttaacaga
     2521 ttctaaaggt agaaaagtgg actttagaaa tacaatcatg attatgactt caaatatcgg
     2581 agcgacacaa attcgagaag aaaaaaatgt cggattcaat gtccaagatg ttactaaaga
     2641 tcacaaagcc atgcaaaaac gtatcttaga agagttaaag aaagctttcc gacctgaatt
     2701 cttgaatcga atcgatgaga ctgtcgtctt ccattcattg agtaaagatg aaatccatac
     2761 aatcgtccaa atcatgagca aagcgattat caaacgattg aaagaacaag atattcaagt
     2821 gaaaatcaca ccatctgcaa tcgatgtgat cgggaaagct ggatttgatc ctgaatatgg
     2881 tgcacgtcca atccgtcgcg cacttcaaaa agaaattgaa gatcgattga gcgaggcttt
     2941 gcttggcgga gaaattcgtt taggtgacca cgtaacagta ggtgcttcaa aaggtaagat
     3001 cacattgaat gttcgcggac ctaaaaagga aacagtaggt tccctatagt tacttggcct
     3061 gtgtagctga atttttataa tagtaagact tgattaagag gttgtgacaa gatttttgtc
     3121 acaatctctt tttatatttg gacagaatat atcgaacttt attttgattt tttgtgaaat
     3181 aattttcaaa aatccgactt taaatagaag aagtattgga aagtttttag agctatgcta
     3241 ctattatgaa gttgtaggag gagaaaatta tgatcgaact cataaccaca gtagaaacta
     3301 ttgaacaagc agaacagttg cttgctgcag gtgcagatac actatatttc ggtgaagagc
     3361 aatttggatt gcgtctgcct gcttcattta cacgagaaga acagcgacaa ttggtagaat
     3421 tagcacacaa gtatggtaaa aaagcgaata ttgctgtaaa tgggatcatt catcctgaaa
     3481 aaatgaaaaa tgtacctgaa tatttggctt ttttaaaaga aataaacgta gatgtgatca
     3541 cagttggtga tacaggtatt atttatacga tgaaaaaaaa tcctgaattg cgtataccgt
     3601 atatttatga tgctgaaaca ttagtaacga gcgcacgtca aatcaatttc tgggcgaaaa
     3661 aaggcgcagt tggagcagta cttgctcgtg aagttccttt tgaagagatg aaggcgatgg
     3721 aagaaaaact cgatattcct gtagaaaccc ttgtgtatgg tgctacatgc atccatcaat
     3781 ccaaacgtcc actgctacag aattactata actatacgaa acaagacgaa caaaaagatc
     3841 gtgaaagagg tctttttatc tcggaaccta aaaaagaaga gacacattat tctatttatg
     3901 aagatagtca tggcacacat atttttgcca gcaatgatct gaatctctcg aatgaattga
     3961 atgaacttta tcagcatcat tactgtacat ggaaattgga cggattattt actccaggcg
     4021 aaaaattcgt aaacatcact cgactattcg ccgaagcaaa aaaacaaatt ttagaaggta
     4081 gctgggatca agaaaaagcg gaaaaaatga ctagcgaaat cagtaaattg catcctgaaa
     4141 accgtgaatt agatacagga ttcttttata ttgatccttc tgctatcaaa taaggagtat
     4201 gatgaaagaa atgacagtaa aaagaaaatt gaaacgacca gaaatcctag cgccagcagg
     4261 aacactggaa aagttaaaaa ccgcgatcca ttatggagca gatgcagtat atattggcgg
     4321 gaatgcatat ggtttaagaa gccgtgcagg taattttact tatgaagaaa tggctgaagg
     4381 agttgctttt gcaaaagaac atgatgcgaa ggtatacgtt gctgctaata tggtcaccca
     4441 tgaaggaaat gaagatggag caggggaatt tttccgcagt ttgcgtgatg tcggtatttc
     4501 tgcagtgatt gtttctgatc cagctttgat cgaaatttgt gcgacagaag ccccaggtct
     4561 gccgatccat ttatccactc aagcttcagc aactaatttt gagacattgg aattctggaa
     4621 agaagaaggg ttggaacgtg tcgttttagc tcgtgaagtt tcaatggagg aagtggcaga
     4681 gattcgtaaa aatacggata ccgaaatcga agcatttatc catggtgcca tgtgtatctc
     4741 ttattctggt cgatgcacat tgtccaacca tatgtcgatg cgggatgcaa accgtggcgg
     4801 atgttctcaa tcttgtcgct ggaagtatgg attgttcgat atgccttttg gtcaagaacg
     4861 aaacagttta actgatacag gcgaagtgga agaagaattc tcgatgagtg cagtggatat
     4921 gtcaatgatc gaacatattc cagaattgat tgaaaacggc gtagacagct tcaagatcga
     4981 aggaagaatg aaatcgatcc attatgtatc tacggtgtca aatgtctata aagcggctgt
     5041 agacagttat atggaagatc ctgaaaacta tgtctgcaag caagaatgga tcgacgagtt
     5101 atggaaagta gcacaacgag aattagctac aggtttttac tacaatacac caactgaaaa
     5161 cgaacaatta ttcggtgaac gtcgtaaaat cccagtatat aaatttgtcg gtgaagtatt
     5221 atcttatgat gaggcaacac agattgctac gatacgtcag agaaacttgt tttctgtagg
     5281 agatgaaatc gaattttatg gtccaggatt cagtcatttc agtcaaaccg ttgaagttat
     5341 gcataatgaa gaaggcgaat ccattgaccg agcaccaaat ccaatgatga ttttaactat
     5401 gccagtcgca cagccggtga aacctggaga tatgatccga aaacgtaaat aaggaaaaag
     5461 ctgatatatc cctgttttaa gcatcttcgc atacaaaatt caagaaaagt gttacacttg
     5521 ttttctgtag aaaaaggatc gttaaataaa ggttttgatt gatctcttca ataaggagga
     5581 acaaaatgca aatatatgat tacatcgtaa ttggtggcgg ctctggcggt attgcttcag
     5641 caaacagagc agggatgcat ggcgcaaaag ttttgctgat tgaagcgaat gaattgggcg
     5701 gaacatgtgt caacgtcggt tgtgtaccta aaaaagttat gtggcaagca agtacgattt
     5761 tggaaacaat tgaacgagat gcctcaagtt acggtatcca agcagattta aaaaatgttg
     5821 atttcgccgg tctagtagaa agacgagaaa aatatattga ttttctacac ggtgcttatc
     5881 aaagaggatt agatagcaat catgtcgagg ctataaaagg ttacgctact tttatcgata
     5941 gtcaaacgat cgaagtaaac ggtgaaacct atcgtgctcc taaaatcttg atcgctactg
     6001 gaggccgtgc aagtaaaatg accattcctg gtggagaata cgcaattgat tcaaatggat
     6061 tttttgaatt aacagagtta cctaagagag caatcgtttt aggtgctgga tacattgcgg
     6121 ctgaactttc aggtgtattt cgtggtttag gtagtgaagt catgtgggca tatcgaaaag
     6181 aacgaccact tagaacgttt gataaaatgc tttcggacaa tttgatccaa atgtaccaag
     6241 aagctggaat aaaaacttac gcacatcata ttgctaaaga aattactaaa aacaatgacg
     6301 aatatacggt gctatttgaa aatggtgaaa ccttaactgg tgattgtgta ctttttgccg
     6361 gtggacgtgt accgaatacc gaaaaattag gtctggaaaa tacaaatgtg gaattggaca
     6421 aaaaagggtt tatcaaagta gataaatacc aaaatacaac ggatgaacat atttttgctg
     6481 ttggtgatgt gatcggtaaa ttagatctga ctcctgttgc aatcgcagct ggtagacgtt
     6541 tatcagaacg tttatttaat ggaaagaaag attcttatct tgactataaa cttgttccga
     6601 cagtcgtctt tacgcatcca ccaattgcaa caattggttt gacagaagaa gaagcattgg
     6661 aaaaatatgg cgaaaatgaa ttgaaagttt atcgttcacg ctttacccca atgtactttg
     6721 cattaaatga ttatcgccaa aaatgcgaaa tgaaattgat ctgtgtcgga aaagaagaaa
     6781 gaatcgtcgg cttacatgct attggtgttg gtgtcgatga gatgctgcaa ggttttgccg
     6841 tggctatcaa gatgggtgca actaaagaag actttgacaa tacagtagcc atccatccaa
     6901 caggagcgga agaattcgta acgatgcgat aatttaatat taacagttcc aacagctttt
     6961 tattgaacaa accatgtaaa accagaaaat cagtatactg attttctggt tttttgtttt
     7021 ttagttattt atgtaagatt gcttcgataa ttctcaaaac gaaataagta gggaagttgg
     7081 agattttgat tatcttcgta actgacgaag agtaaatgaa tccatttggt atgcactctt
     7141 aagttcaaaa tattggtatc gatgtttaaa agaaatagaa atgtatcgaa gaacagatgt
     7201 ttaaaaaagt gtgactttgt ataaataaat tagaagaagc aaacgaggat gttaaaagaa
     7261 taccatatag ttaccaaacc aagaaagttc tcgtcaagtt cgctggtacg aatcaaatca
     7321 agtgaaaaaa gggactgtga aaaaaattgt gtcatagtta gtcacttttt ccgaacaaat
     7381 agtgttcaat cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     7441 ttttccattt gtttctctaa tactcaggac aaaacactga ttt
//
LOCUS       DACBZN010000117         7390 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.117, whole genome shotgun sequence.
ACCESSION   DACBZN010000117 DACBZN010000000
VERSION     DACBZN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.117"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVD64_002468"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVD64_002468"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6149683.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IVD64_002469"
     CDS             complement(937..1518)
                     /locus_tag="IVD64_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6149684.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVD64_002470"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVD64_002470"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP6149685.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IVD64_002471"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IVD64_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP6149686.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IVD64_002472"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IVD64_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP6149687.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IVD64_002473"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IVD64_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6149688.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IVD64_002474"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IVD64_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6149689.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         2244 a         1435 c         1501 g         2210 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttgtggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat
//
LOCUS       DACBZN010000118         7381 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.118, whole genome shotgun sequence.
ACCESSION   DACBZN010000118 DACBZN010000000
VERSION     DACBZN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.118"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            138..785
                     /locus_tag="IVD64_002475"
     CDS             138..785
                     /locus_tag="IVD64_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6149690.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1178..3823
                     /locus_tag="IVD64_002476"
     CDS             1178..3823
                     /locus_tag="IVD64_002476"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP6149691.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4133..5446
                     /locus_tag="IVD64_002477"
     CDS             4133..5446
                     /locus_tag="IVD64_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP6149692.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5436..6089
                     /locus_tag="IVD64_002478"
     CDS             5436..6089
                     /locus_tag="IVD64_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6149693.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6144..6815
                     /gene="radC"
                     /locus_tag="IVD64_002479"
     CDS             6144..6815
                     /gene="radC"
                     /locus_tag="IVD64_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP6149694.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6710..>7381)
                     /locus_tag="IVD64_002480"
     CDS             complement(6710..>7381)
                     /locus_tag="IVD64_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6149695.1"
                     /translation="NKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDC
                     QGSVAGQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEA
                     TKQKFEQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALM
                     KELNEIEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNIT
                     VNFLI"
BASE COUNT         2395 a         1246 c         1541 g         2199 t
ORIGIN      
        1 agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac acccttttcc
       61 ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat gtgaaattat
      121 taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca actgcgaaaa
      181 gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata agtagaatca
      241 aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc agagacttct
      301 cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt cttatcgatg
      361 tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta ggatatggaa
      421 atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta aatatcgtgt
      481 gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa atgatctatc
      541 cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca atctctacgg
      601 taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat gtaaccgcta
      661 tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt caatacattg
      721 atttgacaac cgagttgcag acgcttattt actttgatga gaattactca gaagtttttt
      781 cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat tctttcaaaa
      841 attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa aaaaaaaaac
      901 cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc attcttagag
      961 agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt gggagtttct
     1021 gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag aggcaataat
     1081 gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa tacgaaagta
     1141 ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa ataacctatc
     1201 aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt tagaacaaga
     1261 tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa tcccgccccc
     1321 aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc aagatatgct
     1381 gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta tggaccatgc
     1441 aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga tttctcgtta
     1501 tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag agtatgcgag
     1561 ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta gggaacgttt
     1621 tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct tatacgaaaa
     1681 aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac gaacagctct
     1741 atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata tgagctatga
     1801 actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa cgatgttggg
     1861 agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca tcggcaaaaa
     1921 agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat atgtagatat
     1981 ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg actttgaggt
     2041 aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta cgatgaatga
     2101 actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa tcgtgcaaga
     2161 tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg tgggtcattc
     2221 cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg taaaaatggc
     2281 gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg aattcttccc
     2341 tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt gggtaatctc
     2401 acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa caggtgaaat
     2461 gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc ctgatgtttt
     2521 agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc cagatacgga
     2581 aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt atgacatcat
     2641 tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg aacgtccatt
     2701 tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga tgagtaaatc
     2761 attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag atgctctccg
     2821 ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct atgaaaaaat
     2881 ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg ttatcatgaa
     2941 tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag tggctgaccg
     3001 gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat ttgatcgttt
     3061 tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt tttgtgactg
     3121 gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa aacaaaccac
     3181 ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc caatcatgcc
     3241 attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc tagtagtggc
     3301 agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag ggatggaagt
     3361 attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata caccattgtc
     3421 taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat ttttagttga
     3481 aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat caagtgagat
     3541 cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct tcttgccgtt
     3601 agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac tggaaaagtg
     3661 gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg tttctaacgc
     3721 gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag agaagcaaac
     3781 ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga aaaagagagt
     3841 gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca ggaaagagtt
     3901 tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg tcgcatctct
     3961 tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta tcaagagtca
     4021 ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc atacttttat
     4081 ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt tgatgacgat
     4141 tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc ctggccttga
     4201 ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa aaacgatcca
     4261 tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc tattagaaga
     4321 ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca atgaacggat
     4381 cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa aaaaatatca
     4441 gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg aattcgagac
     4501 attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag cgattatcga
     4561 agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc tgacgggaat
     4621 aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag aaatcgctgc
     4681 acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata ttgccccaga
     4741 agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt atgtatacgg
     4801 ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag tatttgaatt
     4861 ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc aacatcaagc
     4921 tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg aaaagctgcc
     4981 tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag gaagaatgga
     5041 gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc atgcaatgaa
     5101 ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca ttttgttttc
     5161 cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag taccaaatgt
     5221 ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg aagtatcaga
     5281 tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag cagatatatt
     5341 ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt tcatttcaga
     5401 agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca gtgatttttg
     5461 acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca caagtagtag
     5521 cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta ggtgtttcag
     5581 atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa acaagtgtac
     5641 aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa ggagcggtac
     5701 aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag attcccaaag
     5761 ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag aacaatctgt
     5821 ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa ccagacccag
     5881 aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca ttgattttag
     5941 aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta gtcatggtac
     6001 cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt gtttcttctt
     6061 tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc ttattttaga
     6121 taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta cctactagct
     6181 ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat caggagcttt
     6241 tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt gctggaaatc
     6301 tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat gagttagaag
     6361 agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc gaattaggtc
     6421 gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc tacgaattgg
     6481 cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt tgtatttatt
     6541 tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg ctgaatcagt
     6601 cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca gcaaggatcg
     6661 ttttagcgca taatcatcct tccggaatat ataagttagt acacataaat tatataagga
     6721 aatttactgt tatattgcca tctggaaata ctattatgtt atcgataatt gaacgccata
     6781 atgcattttt ttcttcttcc gaagattctt tataaatttc atcgaattct tttgatagaa
     6841 taaattcgac attttttata tctattttag ctggctctac ttcaatttca tttaattctt
     6901 tcattaaagc ttctcgatct tttttgtatt cctctaagtc tataacttca ttcaagtagg
     6961 ctttcttcaa tctatctaat ttattctcaa tatttttctt actatttttc tttttaataa
     7021 tctttggttg tttttttgaa tagtttattt gtatttgttc aaatttttgt ttagtagctt
     7081 caaaaatata acgttctaaa tttttttcgt aaaaagcgcg cttatttgtg caacgtaatt
     7141 tattatttct tcgttttaca catctataac aagtcctttc gtatatagat tctgtaccgt
     7201 cttttttctt atatcttgct ttaatagttt gaccagctac tgatccctga cagtcagaac
     7261 attttaataa acctttaaaa atgtaatcac gttttgcatt tgtttttaaa tttttaggta
     7321 aggctttttg aacagcataa aaaagttttt tatcaataat aggaggacag taatttttat
     7381 t
//
LOCUS       DACBZN010000119         7343 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.119, whole genome shotgun sequence.
ACCESSION   DACBZN010000119 DACBZN010000000
VERSION     DACBZN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.119"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..1368)
                     /locus_tag="IVD64_002481"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="IVD64_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAP6149696.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="IVD64_002482"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="IVD64_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6149697.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="IVD64_002483"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="IVD64_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2652..2882)
                     /locus_tag="IVD64_002484"
     CDS             complement(2652..2882)
                     /locus_tag="IVD64_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP6149698.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3112..3930)
                     /locus_tag="IVD64_002485"
     CDS             complement(3112..3930)
                     /locus_tag="IVD64_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6149699.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="IVD64_002486"
     CDS             4091..4780
                     /locus_tag="IVD64_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6149700.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="IVD64_002487"
     CDS             4794..6296
                     /locus_tag="IVD64_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6149701.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="IVD64_002488"
     CDS             6309..6785
                     /locus_tag="IVD64_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6149702.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="IVD64_002489"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="IVD64_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2364 a         1174 c         1337 g         2468 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgtatggaat ggcaacagtt tttttgacaa attttataag gtgcagaact
     7201 tctttccgta tgctattccg attgtccttg acaatgagcc tcctcggatg tgacgatctt
     7261 cggcaggagc taacagttaa agttagactg cctcgctagc gttagcacat ccgagctcat
     7321 tatcaaggat tcgctgcgcc aat
//
LOCUS       DACBZN010000120         7317 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.120, whole genome shotgun sequence.
ACCESSION   DACBZN010000120 DACBZN010000000
VERSION     DACBZN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.120"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(153..1238)
                     /locus_tag="IVD64_002490"
     CDS             complement(153..1238)
                     /locus_tag="IVD64_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6149703.1"
                     /translation="MSTEIQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSQTIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(1558..1965)
                     /locus_tag="IVD64_002491"
     CDS             complement(1558..1965)
                     /locus_tag="IVD64_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149704.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     KNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(1984..2802)
                     /locus_tag="IVD64_002492"
     CDS             complement(1984..2802)
                     /locus_tag="IVD64_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6149705.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(3028..3321)
                     /locus_tag="IVD64_002493"
     CDS             complement(3028..3321)
                     /locus_tag="IVD64_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP6149706.1"
                     /translation="MSINTFVYSHPINVYIIKYLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFTSNQQRKVKKQID"
     gene            complement(3457..3636)
                     /locus_tag="IVD64_002494"
     CDS             complement(3457..3636)
                     /locus_tag="IVD64_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149707.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
     gene            complement(3633..3983)
                     /locus_tag="IVD64_002495"
     CDS             complement(3633..3983)
                     /locus_tag="IVD64_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149708.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMRTE
                     EIAEILNASVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3976..5301)
                     /locus_tag="IVD64_002496"
     CDS             complement(3976..5301)
                     /locus_tag="IVD64_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6149709.1"
                     /translation="MLDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKNLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIAPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     SITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            complement(5410..5613)
                     /locus_tag="IVD64_002497"
     CDS             complement(5410..5613)
                     /locus_tag="IVD64_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6149710.1"
                     /translation="MKEQQLGYTLWGRPLYGLERRTAVSIDCACGELIQLNIQTKQYN
                     CKNCGNCYQFIDQDFQLLNEKNK"
     gene            complement(5773..6348)
                     /locus_tag="IVD64_002498"
     CDS             complement(5773..6348)
                     /locus_tag="IVD64_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6149711.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKLFAKKNNPNFKEGRPKATITPKKRHAYELI
                     TSGKSYKEVEAITGFSRSTLFRIKKQIEASE"
     gene            complement(6364..6969)
                     /locus_tag="IVD64_002499"
     CDS             complement(6364..6969)
                     /locus_tag="IVD64_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAP6149712.1"
                     /translation="MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTF
                     KGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPVMYLEQSLNHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         2131 a         1346 c         1077 g         2763 t
ORIGIN      
        1 ttaaaaccta cgtttgtgat tgttttcata aacttttaat ctaaaaactc cggaataata
       61 actatttatt tgtaacattt gtaataaaat taaaattata aaaaagttct aatttaaaat
      121 tgatcatttt tatagtgatc gctttttatc aactactcta gccaattttc cgtactaact
      181 gttggtaatt cgttaaatgt ttctctttgt tcgtttgcaa gctttgccgc aatagctttt
      241 tcaataaacc agttttttac atacacaaaa atcaaattct gcaaattatt tactttctca
      301 gtcttttgtt tttgataagc tttcagcaac gtcatatata atcctttatc cgcatcaatt
      361 ccgtaagatt caagttcttc atacacaact accgtcttct caagttcctc ggctttatgt
      421 tttgcgttgt gtattgtggt gacggtttgt tgggcttctt cgtaagtgtt agaaaatgtt
      481 tttatcaagc ttaatacctt ttctggtaca aatgtgttta tgctgtcttc tttcatgatt
      541 tcaacgaagt tatctagcaa aacttgattt tgtaattggt ctgtttcagt gtcttttaag
      601 tgtcttttag tgtctaaaga tttattaata taataatctt gtgcgatttc cgcatttcca
      661 gtgttatcaa gggtttcagc tgttttttct cgacgtgcga tttccgcatt tccagtatta
      721 tcaagggttt tagcagtttc ctctcgacgt gcgatttccg cattccgaga agaggttgat
      781 ttatcaatag tttggcttaa atcgtcatcg aaagaattct gaataaaaag atcatcggca
      841 cttatttcag gttgaagtaa atagtattta tttttttcat tttttttcgt ttttgggtta
      901 aattctcctt ttacgactga aatcaaatta tatgtttcta actcgtttaa aatggttttt
      961 actttgcctt ggtagcaatt taatttgtcc attaggtctg cttgtgtaaa ttccaaatat
     1021 aatagaccat catcgtcgat ccatttttca tgtaaggctc tacgaaattc atttttaaaa
     1081 agcatatagg ctatttttga gctgtctttc attttagagt atttagaatt ctgaattaaa
     1141 aaactaggaa atttataata tttttcaata taaacattcg ttgctttata tccccaattt
     1201 ttaataatca ttttttgaga ttgaatttct gtagacatta aatttctcct tcttcataga
     1261 aacttgtata tattcacgat tttcaacaag tgaactatac catgtattaa gggtgaataa
     1321 aacactttca gtatttaagg tataagttat atattaaaaa aataagtata agttatactt
     1381 taaatacaaa taatgtataa cttatacttt aaataataat tttataatgt tgatttaatc
     1441 gcaaatttac acgttgttta ttaagttata ctttaaatat ttaatgtata acttatactt
     1501 taatattcat ttatctagta taacttatac tttaattgaa taccgcatga aaaaaaatta
     1561 ttttggtaat ttttcaaact cttcttgtat ttcttctctt atgtgttctt cttttgctac
     1621 tcctagtcta tgctggatat atgagtctgc taatgtatta acaatttttt cgaaagaaat
     1681 tctccctttt tcactttgta actccgtcat atagtcttgt aaaattgaga gtttgctatt
     1741 taatgccgga gagatctttt taggaactga taccttgttt cttttatcag gaattgaaac
     1801 tggtcggcct ctttttttca atgtaatatt ttttgtgtta ctcaatgcac tattgctaaa
     1861 gttatattca ccgcttattt tcacatcttg gcgtttgtta ttgggtaata atggctcaaa
     1921 ttcgccattc tccatcattt taggaatatc aaaatcttta ggcattactt atgcaactcc
     1981 ttattattta tttaaaaact cttgtctagc atctagttct gccaaaatat ttataaatac
     2041 ttggtgtgct ttataatccc attgatcaaa atttccattt ttgtattttc ttaaatgtat
     2101 accaatttta gaataccttc tcaatctttt ttgataatga atgagatttt tggataccac
     2161 ggttccgtat ttttcaattg cttcttctag gatttcttca tcaaaatcat catttggttc
     2221 taccatacaa ggtacaatat ctataatttg caaatcaaga tcgtatctat cgaccatgaa
     2281 tttttgataa tttacatatt tgttgactcc ctctaaggat tcttcagctg tttgaaatga
     2341 aattatcgaa tagtctgaag ctgccatagc attatcggaa aattcgctaa tggttggtgg
     2401 tacatcaata aatatttctt gataattgtt ctttattgga agaagtaatt ttttcaagac
     2461 agtaacttgg tctaattcat tttcatgttt attagcaaaa gcggagaatg atcgaaaact
     2521 agtgtcacat gccagaaggt caagattttc atttacctta ataatactac ttgctaattc
     2581 tccatttgcg acaccatttg ttatagtggt tttgatatcg gttataccga aagttcttgc
     2641 tatgacttgt gttaagttcg cttgagggtc taaatcaata ataagcgttt ttctatttct
     2701 tatcattgca ttgtcataag caagcatgct gactatctta gttttaccta ctcctccttt
     2761 aaaattgcca actgtaaatg tagtcccttg aaaagtatcc atgtaaatcc tcccaatctt
     2821 taattgataa ttaaagtata acttatatat cggaaatggt caacattaaa agtataactt
     2881 aaataataat atctaaaaac gtttggatat agcggttttt aagaataaaa gtataagtta
     2941 taccctaaat atatatggta taacttatac tttaattatt taaggtataa cttatacctt
     3001 agatattgct tttcgaaaat agctattcta gtctatctgt ttctttactt ttcgctgctg
     3061 atttgaagtg aattgatcat attcattttc tagtattaat aacttttcgt atacatcggt
     3121 catattcata ctagaagcaa gacttagatc atatacaaat gaagcaggta atgattttat
     3181 tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca
     3241 aaattgttct acagtgattc ctaaatattt gataatgtaa acattgatcg ggtgcgagta
     3301 aacaaatgta ttgatggaca tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa
     3361 taatagttta acacacgcat tattctagta tctactccta tttgtaaaaa caaaaaagga
     3421 ttcatagaga taactctata gaatccgtaa aataccttat ataaataacg ggaaaatata
     3481 aaacctaaaa ttatgataac ctgctgcagc ttctacatag aaagagaaac gctcaacacc
     3541 aacctcattt atcaaacgtt ggccataccc ccaaatctct tctgatagct gatcaaaggt
     3601 tatatacttt agctcatatt gctggataat gttcattcta atcgactcca tttattatcc
     3661 tggtaaaatt cgatatgacg tatttcgtcg tagtgaattt tgtctttgcc aacgtagatt
     3721 cccagctcat cactacctaa gatctttcca acaatatcgt ctggatagta tccttcagcg
     3781 tttcgttctt ctttttgaat acatactgat ctatttttta tgactgaagc gttcaatatt
     3841 tccgcaatct cttcagtcct catttgttcc tttgctaaat tttgatttgc acgttgctca
     3901 ttatctgcat cgatctcttc agtatgatcc gataaataga aacctgccca tttcaatcgt
     3961 cctcgatcgt tatagtcaat catttttaag cccccctgcg ccgcctgcat ggccaccaac
     4021 caacgtgctt ctagtaatac tacgagcgcc ttctaacatc gaagaagcat gtacaattga
     4081 cgtaaaacca tacttctgcc taattttatc tattattttg tctagttttt cgtcatcaat
     4141 ctgtttttgt ggatcactaa acaaatccag ctgaagcgaa tcagtataaa ctagcttgct
     4201 gtaagtgatt ccgacatggc ggacttcttg tccctcatag tatttattaa ataagaacag
     4261 acaatagtct tgtagctctc ttgtattatc tgtgatagat atcttcatct ggtgtgaaaa
     4321 ccctggtctc gtttctaaaa tcgacgttcc aatgtgtaaa tgaacacaac ctgttttaca
     4381 gtggtgttgc ctaatacgga ttgctacttg ctcagccatt tcttttacga ccagttcgat
     4441 ttcatttctt ctagtataat ctcgtggtag cacttgtgaa ttgccatatg atttttcctt
     4501 tgtcggttct ggtgcgattg ctatgtcagt tcgatcaata ccatttgcat ggaagtacag
     4561 ctgtaaccca atcacaccta aacggctctt aatggtgtat ggattccaat tagctaaatc
     4621 tttgatactc atgatcccca tttggttgag cctttttttc attcgagaac caatacccca
     4681 aaagtctgtc atttcaggaa tattccatac tttatcagga acattttgat acgtccattc
     4741 ggcaataaat ccttcattat gcttcgcttc attgtctaag gctagtttag ccaaaagagg
     4801 attatcgcca acacctactg tagcaatcaa tcctagctct tctttaatac gttcttggat
     4861 catttgagcg agattcttcc ttcgctgact tcgtgttcct tcagtcgtaa aaagattcag
     4921 tgatcgggtc acttttaaga ttgattcatc gatcgagtaa atcagtagat cttcatcagc
     4981 cacatatctt ctgaaaatat tatttacctg catattacgc ttaatataaa ggttcatacg
     5041 tggtggaaca acatgtaatg ttttagggaa aggctgtggt agatcccgtg gtctgctgac
     5101 attagaaata ccatatttct tctttgcagc tggtgaagaa gctaaaatca accctgatcc
     5161 tgtattatcc gctcgactca tgacaactaa ttcagtcgtt aatggatcta aatttctttc
     5221 tatacattcg acacttgcgt aaaaactttt catatcgatt agaaagtagt cattgactgg
     5281 ctcttttgag taatcaagca taaagttctc cccctcttct ctcatattat acaaacatac
     5341 gttcgtgttt gcaacgagga tcttctctaa atagaaaaat agccagacaa ttgtctgact
     5401 ataataaacc tatttgtttt tttcgtttag caattggaag tcttgatcaa tgaattggta
     5461 acaattccca caatttttac aattgtattg tttcgtttga atattgagtt gaatcaactc
     5521 accacaagca cagtcaatcg atacagccgt tcttcgttct aatccgtata atggacgtcc
     5581 ccaaagagtg tagcctaatt gttgttcttt catttacttc gttcctccct aaaaatatgt
     5641 ctattcgtag ctggtatgac gcttcgttcg accaatgata catcttaatg agtgaacctg
     5701 accttagaat gctcgtataa acaacgattt atttgattta aaagagattt ttatattaat
     5761 tttatcaatc ttctactcac tcgcttctat ttgtttctta attctaaaaa gtgtactacg
     5821 actgaaaccc gttatggctt ccacttcttt atacgatttg ccggaagtaa tcaattcata
     5881 tgcatgcctc ttctttggtg tgattgttgc tttcggacgt ccctctttaa aattaggatt
     5941 attctttttc gcaaataatt tcccttcctg agtacgttct acaatcatat cacgttccat
     6001 ttcagctaca ctcaacaacg tacgtgtaac cattcgaccc atcgatgtat tttcgatcgt
     6061 ccctagattc aataccttaa tcgtgacgtt atcatcgagt aaaggttcaa tgatctctag
     6121 tgcttctctt gtattacggg cgaatcgatc aagtttcgta acaacgattt cgtctccagg
     6181 cttcagacca gccaacagct gattaaactt tggccgatct cgtttcgtac ctgtaaattt
     6241 ttcagaaaaa atattcttct tctcaacacc gttctcaagt agctgttgta tctgttcctt
     6301 tgtactttga gaagcggttg agacacgtgc atagccatat tttgtcattt aactcacctg
     6361 cttttacaaa tcatctatcg tatactcaga gtatccttca tagaagtagc tgacatcgat
     6421 tcctttcata tataactcac gatcgtcaat tttatcagtc agggcattgg aaatgagata
     6481 gcgtatttcc aagtcattta ttggactgcg ctccattgca gaaagatagt ctgctttatc
     6541 gatcaagttc caatcgacca ctttttgcaa ttcttccttt aaaatgagat ccagccaaat
     6601 tctagtactg cgcccgtttc cttcgcgaaa agggtgagcg atattcattt ccacgtattt
     6661 cttaataatc tcttcgaaag aagtttgcgg catctgatca atatgattta aagattgttc
     6721 taggtacatg accggcgcaa aacgaaaatt tccttttgca atattaactg tccgaatttt
     6781 tcctgcaaaa tcataaattt ctgaaaagag aaaatcatga atttcagaca agcctttaaa
     6841 cgtgccgact tcgatttcat ttatttttcc agaatcatat aattcttttg ctttttgctt
     6901 actgagtttt tcttcagcct tggccaactc aacctgattc gttaaaccca atttattttc
     6961 taaaatcatg gaaaatcccc ttaacttttt gacataagtt tatgaaacac tacaaatcct
     7021 attgtatcgc gaatttaacc atgtgtcaaa aactattaag tttttgacac gtagttagtt
     7081 ttcaatagaa gccatctgtc cttttctttt taaatggcaa aaatttttta ttttattcgg
     7141 cacaaactat aaacatttaa tcgctaagaa aatcactcaa ttattttgag tgattttttc
     7201 agttcttatt ttaaaacaaa gtgttcataa aatcaggtgt ttctttattt ttcagtagca
     7261 taccctgttt ttattttgag tattagtaat aattaaaaaa accacttgta attttat
//
LOCUS       DACBZN010000121         6962 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.121, whole genome shotgun sequence.
ACCESSION   DACBZN010000121 DACBZN010000000
VERSION     DACBZN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.121"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..193
                     /locus_tag="IVD64_002500"
     CDS             <1..193
                     /locus_tag="IVD64_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149713.1"
                     /translation="GRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPL
                     VPKGYDFLDSISKLEKMND"
     gene            271..945
                     /locus_tag="IVD64_002501"
     CDS             271..945
                     /locus_tag="IVD64_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6149714.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1178..1612
                     /gene="dcm"
                     /locus_tag="IVD64_002502"
     CDS             1178..1612
                     /gene="dcm"
                     /locus_tag="IVD64_002502"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6149715.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1613..2320
                     /locus_tag="IVD64_002503"
     CDS             1613..2320
                     /locus_tag="IVD64_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6149716.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2310..2600
                     /locus_tag="IVD64_002504"
     CDS             2310..2600
                     /locus_tag="IVD64_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149717.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2859..4043
                     /locus_tag="IVD64_002505"
     CDS             2859..4043
                     /locus_tag="IVD64_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6149718.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4058..4177
                     /locus_tag="IVD64_002506"
     CDS             4058..4177
                     /locus_tag="IVD64_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP6149719.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            4923..6833
                     /gene="ltrA"
                     /locus_tag="IVD64_002507"
     CDS             4923..6833
                     /gene="ltrA"
                     /locus_tag="IVD64_002507"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6149720.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         2437 a         1118 c         1539 g         1868 t
ORIGIN      
        1 gggtcgtatg agtgaactcg gatattccat gatgtttggg gaggtcgaaa aaaacttctt
       61 tatgaagcac atcaaaggtc gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt
      121 ttatacgcca cttgtaccaa aagggtatga tttcttagat tcaattagta aattagaaaa
      181 gatgaatgat tgagtgatct tcaatactga ctttatactt caagtaacgt ataatacttt
      241 tatatagtag gaagaaaggg gataattaat atggaaagta atgattctta tttagagtat
      301 gcaaaagcaa ataaggataa gtttatttct tatattatca aagggaaatc ccccgaagtt
      361 ggacgagacg cagtttttat ggcagggagt cctggggctg gaaaatcaga ggttgccatt
      421 ggattagctg agaattatga taaccatgtg attattgatg cggattactt tcgcacccag
      481 tttcctgatt ataatggaaa aaactctagt ctctttcaaa aagcatcttc ttggttggta
      541 gagcaggcat ttagatatgt cttagatcat ggctattctt ttattctgga tgcaactttt
      601 gcaatattaa gtgctgaaaa aaatatctcg cgggcgttga aaaaacattt tcgagtgacg
      661 attttctatg tttatcaaga ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt
      721 gcagagggac gtcatgtgcc gaaaaaggca tttattaacg cttttttcaa atcaagagag
      781 aacattgcaa aagtaaagag taaacatcca gatgtattgc ttcatatcat aataaaagat
      841 tatacgaata atatttccga agttcattat gctacggata atctagaatt atttttacca
      901 ctagggtata gtaggaaaga tttggaggtt caattacatg actgagaaag aacaagtaac
      961 gaaaattgta aagaaataca ataagagcat tgcggattta tcggaaaatg ctactgcaaa
     1021 agagttcaaa acggtgatta aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt
     1081 agttggatta aataaaaaat agtaggacaa attaataatg tatgagccaa gagcaaaatg
     1141 ttcttggctt ttttctttgc aatgaatgag gtaatgtatg agatttttag atttattcgc
     1201 cggtattggt ggctttcgtt taggtatgga acaggcgagc catcattgta tcggtttttg
     1261 tgaaattgat aaatttgcta gacgtagtta taaagcaatt catgatacaa gtaaggaggt
     1321 ggaaatgcat gacatcacaa gtgtatcaga tgagtttatt caatccctcg gaccagtgga
     1381 catcctttgc ggcggatttc cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt
     1441 ttccgatact cgaggtactt tattctttga aatcgctcgg ttcaccactc ttctccgacc
     1501 taagctttta ttccttgaga acgtccgagg attgctcaat cacgaaggag gggctacgtt
     1561 cgagacgatc ctccgaacgc tggatggatt ggggtatgac gtggagtggt aggtgcttaa
     1621 ctcaaaggcc tacattcccc aaagccggga gcggatattc cttatcggac attctcgaga
     1681 cgcatgtacc gaacaagtat ttcctatcat tggaccgtct tcaacatctg atcaaaacat
     1741 cagaaatttg gtaaatatca atccttccaa tcgaggaatg ggtggacaag tctatggttc
     1801 agatggcgta gcgccaacct taacaggtga tgaaggaatc aaaatcgcac tgccagcaag
     1861 ggatggaatc tctgtagcag gactattacc gggaaagttt gaacaaggta atcgagttta
     1921 tgaagttact ggaaccgccc ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat
     1981 tcgaaaaaat gaaacagaat atcaagaggt gaagccagaa gacagtgtga atctggcttt
     2041 tcctaattcc acaagtcgcc gaggtcgtct gggaaaacag agtgttcata ccttactaac
     2101 aggagaccaa caagcgatcg tcacggatca gtatcaaatt cggaagctaa cacctagaga
     2161 gtgttggcga cttcagggct ttcccgattg ggcttttgat cgagattctc aagtcaattc
     2221 agatagtcag ttgtataagc aagccggaaa ctctgtaaca gttccggtca tttttgatat
     2281 tgccagaaga ttaaaagagt tggatgtaca tggtttatga tgagttgatt ggagaaattt
     2341 attgggtgat tggaaagatt cagtcagacc cagaattaga agaacaattg cgacggttaa
     2401 aatttgatat tcgcaagaat ggtgtcaaag tcgctggcga tccatattta atgaatgagg
     2461 agacggatgc tcgaattgaa ataaatcaag taattgcaga atttgagcgg attgcggacc
     2521 aagcaaaaga accagatatt cgtcaatact tgttagaaat gaaagcagag ttggaagtaa
     2581 atggtattac agatgagtaa cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga
     2641 ggattcgcga agcagaaatc ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga
     2701 ccatgatgtc tcacccccgt atctaacagg ggggtacaaa aacacaaaaa ataagtaagc
     2761 aaccattaag aatgcttgtt gtatcaaaga tagtaagtaa tttgtatagg tgttaactca
     2821 tttgaaaaag ttaacacctt gaatttggag gtgagaactt ggcacaacgg aatttagatt
     2881 atcggctatt aaaagaccga cgcaacgaat acggcgtttc acaaaataaa ttggctacgg
     2941 cttgtggact tagtcggccg tacttaaatc agatagaaaa tggtggcgta accgcatcta
     3001 taaaaaccat gaggaaaatt tttgaccaac tggaaagctt taatcccgat ttgcctttga
     3061 cgttactgtt tgactatgta aggattcgct ttcccacaac ggatgcacga aaaattattc
     3121 aagaaattct ccatttgaaa tttgagtata tgctccatga agattatgcc ttttactcct
     3181 atcaagaaca gtatgtcatg ggtgatattg tggtgatgtt gtctcatgaa gaagataaag
     3241 gtgtgctttt agaattaaag gggcgaggtt gccgacaatt tgaaactttt ttactcgctc
     3301 aaaagcgtag ctggtacgac tttttcgagg attgtctaaa ggccggtggc gtgatgaaac
     3361 gcttggactt ggcaatcaat gatcgagtgg ggctattgga tatacctgag ctaacgaaaa
     3421 aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt taaaagttat cgttccgggg
     3481 aacttttaaa ggctgatgaa aaggacggca tgggcaatac cttatatatc ggcagtctca
     3541 agagtgaggt ttatttctgt ttgtatgaaa aagactatga acaatatatc aagttaggga
     3601 ttccattaga ccaaactgag acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg
     3661 cttttcatgc gattcaagat ttgttaaaag gtcgtagcat tgagagcacc acgttttcca
     3721 ttatcaatcg ttacttgcga tttgcggata aagtagaagg taaaaggcga accaactggc
     3781 ccttgaatga acaatggggg cgttttattg gacggaatcg gaaagagatt caattaactt
     3841 ccgagccaaa accgtatacc attgaacgaa ccttgaattg gctaggccga caagtcgccc
     3901 ctacgtggaa aatggctaag gagttggatc gattgaatcg gacaacctat atccaggata
     3961 tggtacagaa tgcacgctta tcagaccgac ataaaaagat tttggagcaa caaagtatgg
     4021 caattgaaaa tttgattata tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt
     4081 tttggtattt ggtggcagta tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc
     4141 agcagcggat cgagcaatgg aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt
     4201 tatgtgcgac tcgttcctta gtgaaaagct aaggcactaa taaaagaacg aaaaagtttg
     4261 gttattagga gaactgataa tctgacaggt ggaatgatga agtaacgctc tgaaacgcct
     4321 gccctgagcc tccgacaagc attgtatgag gaaactcaga atgtttgaag ctcggtaaag
     4381 acggctgaaa gataccgtaa tagttgatga acatatatct tctatcgaaa actatccaga
     4441 ggtgggtagc gtatccaaca ggtcgggggt ctataaaata tctatggtta gaatgttcta
     4501 taaggaactg acgaaacagc gaatgtacgg gtctaaattt gaactttgta gaaatgcaaa
     4561 ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat aggttatgaa atgccatata
     4621 acgttacagg cggtatcaag ccagcaggct tatagctagt gacctaagga tatatatgta
     4681 tagataagat tatcggaacg aggaaaggta ttgggttctc atcaagagaa taaactgacg
     4741 aagaacaata agcagttgaa ctaatgctga aaagcagagg gcgaacctaa gaaattcttg
     4801 taatgagaat ggaggaatgc cctcaagtcg tgtatatatt agaaaagtat tatgttgttt
     4861 caataaggat tacgagtaag ataaaaaagc tcactcaatt tcaagtaaag gagtgaagcc
     4921 ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga tttaagacac gctgaatatt
     4981 acgaaatgca aaacaccttt gatgatttat atgccaaaag taagaatgga gatattttca
     5041 ctcatcttat ggatattatc ctatcgagag aaaatatatt actggcatat cgaaatatca
     5101 aagcaaatgc aggcagtaaa acagcaggaa cagacggtac aataattaaa gatattggca
     5161 aattgccagc agaaacagtt gtcaaaaaag taagatacat tgttgcaggc agtccacatg
     5221 ggtatcgacc taagccagtg aggcgaaaag aaataccaaa accaaatggt aaaaccagac
     5281 ctctaggtat tccttgtatg tgggacaggc ttatacaaca atgtataaaa caagtattag
     5341 aaccaatctg cgaagctaaa tttagtgaaa acagctatgg ttttagacct aataggtcag
     5401 ttgaaaatgc aataaaagca acctataacc gcctacaaat cagccagcta cattatgtga
     5461 ttgaatttga tattaagggt ttctttgaca acgtgaacca ttccaagctg ataaaacaga
     5521 tatgggctat gggaatacgg gacaagcact tgatattcat tttaaaaagg atattaaaag
     5581 ctccaatcaa aatgactaac ggtacaataa catatccaga gaaaggaact ccgcaagggg
     5641 gaataatatc cccactacta gcaaacattg ttcttaatga gttagaccat tgggtagaaa
     5701 gccaatggca ggaaaatcct gttacaaaaa actatgtggt tcatatcaat aaaagcggta
     5761 gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac aaagttaaag gaaatgtata
     5821 tggttcgtta tgcagatgat tttagagttt tctgccgcta caaggaaagt gctgaaaaag
     5881 ccaaaatagc aatcactcaa tggattgaac aacgattaaa attggaagta tcacaagaaa
     5941 aaacaagaat tgtaaatgtt agaaaaagat actccgactt tcttggtttc aagattaaaa
     6001 tgataccgag aagaaagaag ttagttgtta aatcacatat aagtgataaa caattcaaaa
     6061 accaaaaaga caagctggta tcacaggcaa agaaaattgc ttctccctca aaagggaaaa
     6121 cggaattaga agaaacaaga ctttataatt caatggtatt aggtatgcaa aattattatc
     6181 agattgcaac gaatgtaaat ctggattgta tgaaactgaa tagagctgta atggctatct
     6241 tcactaacag attaggtaca aacaaaaggg gcagattgag aaaaacaggc agacaactta
     6301 cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct tcggtatgtt gctggcgagg
     6361 gcgaacctat tttccctgtt ggatatatcc aacacagaaa accaatggca aaggtgtatt
     6421 tagcgaattg ctacactcta gagggaagaa aatttatcca caccaatcta agtgttgata
     6481 aattactgat gtaccagtta atgaaattat ctttggaaaa tcgcacaata gaatacgcag
     6541 acaatcgaat ttcgctattt tctgctcaac atggaaaatg tgctataact ctggaagaat
     6601 ttcaacaagc taatgaaata cattgtcatc atattgtacc aactggagct ggtggaaatg
     6661 acgattataa aaatcttatt ttggtaaaag aggcggtaca ccgtctaata cacgctaaaa
     6721 cagaagaaac aatacaaaaa tatatagctc tattgaagct aaataaagtc cagctcataa
     6781 gactaaataa atatagagta ttagctggca atcaagaatt aaacttgata taggtctact
     6841 acataaatac ttttcaatat aaacacgatg gaacgcggag tgcggtgaaa gtcgcacgct
     6901 ccgtgtgaat ggggggaaaa gtttgagata tttataatat caggaactta cctatccata
     6961 ta
//
LOCUS       DACBZN010000122         6671 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.122, whole genome shotgun sequence.
ACCESSION   DACBZN010000122 DACBZN010000000
VERSION     DACBZN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.122"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..270
                     /locus_tag="IVD64_002508"
     CDS             <1..270
                     /locus_tag="IVD64_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6149721.1"
                     /translation="TKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLEN
                     GAKMKEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(491..1633)
                     /locus_tag="IVD64_002509"
     CDS             complement(491..1633)
                     /locus_tag="IVD64_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6149722.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1638..1823)
                     /locus_tag="IVD64_002510"
     CDS             complement(1638..1823)
                     /locus_tag="IVD64_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6149723.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2177..2422)
                     /locus_tag="IVD64_002511"
     CDS             complement(2177..2422)
                     /locus_tag="IVD64_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6149724.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2416..2817)
                     /locus_tag="IVD64_002512"
     CDS             complement(2416..2817)
                     /locus_tag="IVD64_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6149725.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3002..4810)
                     /gene="uidA"
                     /locus_tag="IVD64_002513"
     CDS             complement(3002..4810)
                     /gene="uidA"
                     /locus_tag="IVD64_002513"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP6149726.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(4833..6599)
                     /locus_tag="IVD64_002514"
     CDS             complement(4833..6599)
                     /locus_tag="IVD64_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP6149727.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         2100 a         1303 c          872 g         2396 t
ORIGIN      
        1 acgaaggaag acggtgagct agttacgcca agtgtagtga aatggaatac taggaggata
       61 tcgaatgcac tctccctctc ttttaacttc cattctctca gacatactca tgctacactt
      121 cttctcgaaa atggcgcaaa aatgaaagaa atctctgaac gattgggcca cagcagaatt
      181 tcaattacga tggatactta ctcgcatgta acagataaga tgagaaatga aacggtcgat
      241 atcatggaaa atcttagaaa gaattcttga tttttgccac cgaaaaaaac caccggtggc
      301 aaaccggtgg caaatccatg taaacatggt ttatttttta cactgttttc aaacaaaccc
      361 ttgctatttc ttcggttcgt cctcgtccat tttcgatacc gacagctagc ttataaccca
      421 aggtatatca acataaaaaa aaaaaaaaaa taggctgtga cagcagcgtg acagcaaaaa
      481 agatggaaag ttaaatatca aacctcgtaa tttcatttgc gagtgcttga tttgtttctg
      541 gatataagtg tccatatttg tccaaagtag ttttaataga ggcatgacct agacgcttag
      601 atattgcata gatattttta ttatgttcaa ttaaaaaggc aacatgcgag tgtcttaatc
      661 catgcatatg aattggtttt actcctgcaa tttcagctaa tttttttatt ctgcttaaaa
      721 tagttcttgg agatggcggt aatccatcaa aagaaaaaac caattctata tttttcccaa
      781 ttgattgttg agtttttttc catgttttta aatcttcaac ggtttgtctg tctaacgaaa
      841 ttatccgaat acttgatgta ttttttgttt cagcaaattc ataatcttgt cttgtacgga
      901 tgtaaattga cttatttacg ctacatctgc catttattaa gtcaaagtct ttccattgta
      961 acgctaagag ctcctcactc cttacacctg taacaaaaat aaaacgcatc aaacgtttat
     1021 aaaattcttc ataaaaatcc ccgtgataaa cactattata tactttctta aattcatcaa
     1081 ctgtccaaaa ttctacttga ggacgctgag tttttatttt acctatagta tctacaggat
     1141 tttcttcaat aatttcaagt acaattgccc ttttaaaaac tactctcaac ttattgaata
     1201 tctgtttaat ataatttggt gataatggtt gctcaatgcc cttttttaca acatggattt
     1261 tcgataaaaa ctgttgaaat tcctgtatat gaattggtcg aatctttaca acctgcattt
     1321 ctccaaaaaa atccaatgca cgttgtaaaa tatgatctgt tttcatatat gttttttcta
     1381 cagttcccaa cttataccaa ggaaaaaaat actctcgata aaaatcatga aacgttattc
     1441 cttgcagaca aattccttgt gaatcagaaa aactttgttt taactcttcg agtccccttc
     1501 tagcttctcc ttgagttttg aagcctcttc tggtcacttg aattcttttt ccaaagcgat
     1561 cttgtcctaa agttgcagaa aaaaaccaag tgcctctttt tctgtcttta taaacattcg
     1621 caattttcct cattttacta ttcctcccaa gaaatatgga gaacttcttc taaaattgaa
     1681 ataggaattc tttttatttt tgaattacga taaaaaaagt atcctttctc aactaaaagt
     1741 tctttacatt ccttgaagat tttattagca tagtactgag aaaaacctag attaatcaaa
     1801 tcttttcgag tagctgtttt cattatagca ccttccttac tttttttcat ttatttctat
     1861 cagctgcttt tgtcaaaagc acataggaat ttcacctctc cccaagttta cggcgagcct
     1921 tacggaagta tcattatccc aactttaaat catcgctttt tattgactat aaaaattatt
     1981 ttagaatatt tatcgctcct tctttacagc aagaaatcat ggcgcaacta aaatatgctc
     2041 cttaaagtgt taacgtactg atagaatcta tttaattttc aatgaacaat aggttttcta
     2101 cctaaaagaa tctactaatt ccttaactat tagaagattc ttttaaacag aaaaagtgga
     2161 aaggagattt ttttaattag ttaattttaa attttaagat cgcttctatt aatttaattt
     2221 ctagctgact tatcatatat tcatctacat aggtaacgaa ttgacctacc tcgttaaatc
     2281 catttgtcaa acataactta gccatataac tatgataatg tttgagtatc atcttgatcg
     2341 aaattggatc tccttctaga gcagataaga taatggatag tggaactaat aggtactgcc
     2401 gctttttgga tttatctacc ataggtcata ccccattttc aattgttcaa ctgccttctt
     2461 tcgttgatac cagataccac ttgtgctcat attgagaatt ttaccgattt cagcatcatt
     2521 aaaacctgca aaataataaa gtaggataat ctttcttctt atctccgata actgatcaat
     2581 tgctgctgat aacttttgat ctgaaattaa tatttccatt ccaaataaga aatagacatc
     2641 ttgttcatca aaatgagttt gggaatacct gccttcattt gaatctgcca atggttcctc
     2701 attatccttt atgttagcat ttcgttttcg aatagactta gcttcgtttc gtaatacttt
     2761 tttacagtag gcatcaaatt ggtgttgtat agatatgtca ttgtaatgtt gttgcatgat
     2821 aatatgctcc tttttccccc tttccactaa tacaacaatc agagagcccc tttttttcga
     2881 aaaaactaga aaaaaacata aaaatattta tcattcttta aacagaaaat ctttctataa
     2941 aaaacaaaaa aacgattagg ctaggctaat cgttttttga acattcttaa aaaaatccaa
     3001 attatttttt ataactgaaa ttgggtattg atgaccatcg tttctgtaga tagtgagcaa
     3061 tcattttagg ttttctatct ctagtaaaga ttcctttctt atttccttgc actcttaaaa
     3121 tcccttgact cgttgcaaaa tctgcaaaat tccacacttg ttcaccaaca aaattttcac
     3181 atgtatctat tacttcgtga tttgcttcgt aataagccac ttgatactct tctgtaaaca
     3241 ttgttggtac tgtatcatga aatccagcta ctgtatctgc tccatattct gtaaacatta
     3301 ttggcgtatc aggacatctt tctttccatc catctagctc ttttttcaac agatattttg
     3361 catgtgccag atcccctccc gaaacatacc aaccataata acgatttaaa cataaaacat
     3421 cgacaatatc tcctacctta caaatatctg gcgttgattt tagataagta actattgtta
     3481 caggtctttt ctgcggatca agttctttag ttaattcaac taaaggttca aaatattctt
     3541 tagcaccttc ggaatccgaa tctggttcat ttgcaataga ccacattaca acacttgggt
     3601 ggttcttgtc gcgttctatc aattccttga tcaccgtttt atgagaattt tctgttcgta
     3661 tctctttcca cgtattatgt ttttccacat tatcatctaa taaagtggcc atgaaattta
     3721 aatgtaaccc tactgcaggt acttcatcaa taactacaat accttctcga tctgctaatc
     3781 gcataatttc ttctgagtaa ggataatgtg atgttctaaa agaattagca cctatccatt
     3841 tcattaaatt aaaatcttta atatttacag cctcattcaa accacgtcca ttaacatacg
     3901 aatcctcatg cttaccaaat cctttaaaat aaaaaggttt attattaatc aaaaatttgc
     3961 cattggtaac ctgaacactg cgaataccaa attcttctgt ataggtatcg atcactttat
     4021 tatcatcaat taaactaatt ttcatactat ataaataagc atctagaggt tcccaaaaaa
     4081 ttggattctt aagctgaatc attcctttct ttccttcagc ttcagctaca agttgagcct
     4141 cttcgtcata tatcgctatt aagatttttc cggtccattc tgtatcgata ttgaattcta
     4201 cacttgcatc caaatcagtt attcttgtat taatagtaat atcacttata tagtctttcg
     4261 gagttgtata taacttaaca ggtctatgaa tacctgcata attgaagaaa tcaaaattaa
     4321 ctaagttttc cttttttata tccccatttt cctgtactgt ttccttcata atccccacag
     4381 gtaaagtact atcgtcaata atattgttta cagcaactgt aattcgatta ttcccgtctt
     4441 ttacaaaccg attaatttca gactcaaatg gtgtaaaccc acctttgtgt tgagtaatca
     4501 actttccatt caaaaaaact tttgcttcat gtgttgcaga tccaaaacga agaactaatc
     4561 tttcttctaa taatctttta ggaagagtca cttctttttc ataccacacc catccaacat
     4621 gatttctaat gttctctgtt tcaaataaat cattataaga agagggaact gaaatcggat
     4681 aactctcttt ttcatttatt ggttctaaag caagttcttc ctgtaagtca tcatttgtat
     4741 ctaatttaaa tctccagaga ccgcttaagt caattacttg cctcgtctct gtcataatgg
     4801 gatacaacat ataaaccact cctatctaaa tattaaataa atgtatcctt taaaccacaa
     4861 taagcatcta ttggacttac tcccttaaat tcagaaagac ccattagttt gtccaccaat
     4921 atttctaaag tttcttgttt tccatcataa gtattaatat aagtctttac ttgaggtaca
     4981 tcagccaaat gaaacggaca ctgtacagat acaaaaattg ttggaatttc atgaatgtaa
     5041 aacggtatat ccggagttcc tttacttgct ggccagacaa ttcgttgaac tgtgcctgga
     5101 ttaacattag ctatattaat gatcaagtca tattttttag ttaaattttt aattggtctc
     5161 ttagcagcat atattccagc tatattttta attttttctt cttgcgataa tcctttcaac
     5221 tcttcttcag gtgaaatgta tttttcaact tcaaatcctt ttgctgacaa aatctgtttc
     5281 aataaatcaa tggcttgagg tctatttcca gcaactactt caccaaaccc accttttacc
     5341 ccatgtacat ctactagtaa aatcctttta tatttttctg gttcaatagg ccaaatatct
     5401 tgtttatctt taactaatgt aattgcttgg tcagccacct ctttaaaaat tgatttattt
     5461 tcaattaaac caattttttc cagagcttct tgcttaggta ataaaatttg ttcttttgct
     5521 tttttgtgta atcccaattt agcttttaac cctaaaattc tttttaaggc atctaataac
     5581 cgatcatcag agataattcc tttacgataa ccttccatca tataattaaa gtcttcatct
     5641 ggatcattaa aaaatagaaa tagatcacat ccagctgcaa tagaagtggg caataattct
     5701 gatcgtttca ttgccccagt cattcctaac atatgactag catcagtaac tactaatcca
     5761 ttaaatccga gttttcctct aagtaagtca gtgattaatt cttttgataa agttgcagga
     5821 agtaaatctt catctttcag atcaggatta aaatgttttg aataagctgg catatggata
     5881 tgacctgcca taatcgaagg taatccagct tctattaaac ctgagtatac ttttccaaaa
     5941 gaattatccc attcttccgt acctaaacta ttaatactaa aagatagatg ctgatctcgt
     6001 tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca aaattccact ctcctgaata
     6061 cctttcatat aagctaaact aagttctaaa actttatcag ggttttgtcc aaaagatcga
     6121 ctagaaatga taggatttct ccaatttcta ttaatatcta caataggagc aaaactccag
     6181 ttacatccaa tagccgatgc ttcaatccca gaaactctcc ctaattcata agcatatttc
     6241 gcatcatttg ttgcaccaat ttttacttct acaccaactt cagtaccatc tgtacaagct
     6301 ccgttaccac ctgcttccgt attagcagca attaataaag gaattttgct ttctgtttgt
     6361 aggatatagt tttgatcata tacctcatca gcagttcctg ggttatatcg tacagctcca
     6421 atatggtact gattgaccat attactcaag taatttttat cacgactaga tcccatatta
     6481 acaaacaatt gacctatttt ttcttccaaa gtcatagaat caatcgtctc ttcaatccaa
     6541 gaaatagcat cttcatctaa attatatggt ttttttcgta agtctacttt tatagtcatt
     6601 ttctttctcc tctacttatt agtaagattt ttccaaaaag tatctataaa tttttgttca
     6661 atatctacat a
//
LOCUS       DACBZN010000123         6646 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.123, whole genome shotgun sequence.
ACCESSION   DACBZN010000123 DACBZN010000000
VERSION     DACBZN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.123"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            41..124
                     /locus_tag="IVD64_002515"
     CDS             41..124
                     /locus_tag="IVD64_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6149728.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            249..986
                     /gene="erm(B)"
                     /locus_tag="IVD64_002516"
     CDS             249..986
                     /gene="erm(B)"
                     /locus_tag="IVD64_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6149729.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1156..1416
                     /locus_tag="IVD64_002517"
                     /pseudo
     CDS             1156..1416
                     /locus_tag="IVD64_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1579..2244
                     /locus_tag="IVD64_002518"
                     /pseudo
     CDS             1579..2244
                     /locus_tag="IVD64_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2280..2525
                     /locus_tag="IVD64_002519"
     CDS             2280..2525
                     /locus_tag="IVD64_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6149730.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2628..3497
                     /locus_tag="IVD64_002520"
     CDS             2628..3497
                     /locus_tag="IVD64_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6149731.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3478..4212
                     /locus_tag="IVD64_002521"
     CDS             3478..4212
                     /locus_tag="IVD64_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6149732.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4245..5160
                     /gene="ant(6)"
                     /locus_tag="IVD64_002522"
                     /pseudo
     CDS             4245..5160
                     /gene="ant(6)"
                     /locus_tag="IVD64_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5157..5699
                     /gene="sat4"
                     /locus_tag="IVD64_002523"
     CDS             5157..5699
                     /gene="sat4"
                     /locus_tag="IVD64_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP6149733.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5792..6586
                     /locus_tag="IVD64_002524"
                     /note="aph(3')-IIIa"
     CDS             5792..6586
                     /locus_tag="IVD64_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6149734.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2329 a         1005 c         1387 g         1925 t
ORIGIN      
        1 tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat tccaaatgcg
       61 taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt acgcagataa
      121 ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta aacagataac
      181 taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat aacttcagga
      241 gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac
      301 tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa
      361 caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg
      421 aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac attcgtgtca
      481 ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg
      541 ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc
      601 atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc
      661 accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc
      721 cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc
      781 ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg
      841 tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg
      901 ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt
      961 atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt tggaaagtta
     1021 cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc ttccaagaag
     1081 ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca aattcccact
     1141 aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat tgtattaaaa
     1201 aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc tgaacatttt
     1261 agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa agaaaccctg
     1321 gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa aattggggaa
     1381 aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc caaaagtagc
     1441 tcctatatga aaaatagagg tctttccatt tagatagtag tggaaagacc tctatttatc
     1501 ttttgaaagt gaatacaaaa ataaagcaca ctctgctata atataggtga ttaatccatt
     1561 tgagagaggt gctttatcat gtatcaaaat tataccataa accaactttg tttgcctata
     1621 gatcttgaaa ttaaattaga agaaaatgat tttgctcatg ccattgtgca gtttgttgac
     1681 tctattcccg atgaagtctt tcttccttat tatcaatcta tgggtagacc ccaatatcat
     1741 cctcgtatgc tactttccat tattctttgt gcctatattc aaggggttta ttcgggccgc
     1801 caaatacaaa atatgctgat agatagtatt cggatgcgct acttatctca agagcaattc
     1861 cctaactttc gtactattaa tcgttttcgc gttcatccga ttatgaacga tattctagat
     1921 cattcatttg ttcaatttcg tgagctactg gttcaaagtg gcttaatcag tggcagtgct
     1981 ctatacattg atggtacaaa aattgaagcg gatgcgaata aatactcttt cgtttggaaa
     2041 aaaagtattt taaagtataa ggatagcctt gatcaaaaag cccttgaaaa ctaccaacaa
     2101 atggtggaaa caaaaatctt acctgagtta atagatgatt taaaggatga actttctata
     2161 gaagagattg aaaggattcg tcaatctttg gaagaaaagg aaaatcaatt aattgaagcg
     2221 attgaccaaa ccgaaacagt agaagggggc tgacccatta ttaataagga gtggacaaca
     2281 tgattgatgt aaatgagcta ttggaagaag ctattagaga aactgaaaat ctaaatgatg
     2341 gcgaagtttt tcttgttaaa gatttattca agggatatgt ggggaatagg atacccagaa
     2401 aggatcgact tctgcttgga acgttatttt taaaccatgt tagcaaaatg aatggtaatt
     2461 taaaggctat tgaaaagact tcatctaatc agcagaggta taaaaagaca attgataagt
     2521 tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg ttgagtagaa gcaaaagatt
     2581 tggcttggaa tttttacagc agtgaatcga taaaaggagc atgcttgatg gtagataata
     2641 ttattaaatc agtagcagag aaattatcct ctctgtctta tatagaaggt attgttttag
     2701 gtggttcacg tgcaaggggc acccatacag aggattcgga tatagatatc ggcatctatt
     2761 acaattcaga atcatttgac ataaatacta ttaatcaatt cgctacaaag ctggatgatg
     2821 agcatagaaa taaccttgtt gtacctcccg gagcatgggg tgattggatt aatggcggcg
     2881 gatggttagt cataaacggg tatcatgtgg atttaatttt acgtgatatt aaacgtgtgg
     2941 aacaaataat gaaagataca gagcacggaa ttgttactgc caattatcag actgggcatc
     3001 cccatggtta tattagtgca atgtatcgag gagaattagc gattagcaaa atactatatg
     3061 ctaagaatga aagcttatgc gaattaaaaa aacaggcaga aacttatccc aatgctttgc
     3121 agaaaagttt agttaacttt tttatgtttg aagcagggtt ctctttaatg tttgtaaaag
     3181 caaattcggg aacagacgat aaatattata ttgcgggtca tgtttttcgt atagtttcat
     3241 gtttaaatca agtgttattt gcatgtaata atgcttattg tatcaacgaa aagaaagcta
     3301 taaaactgct tgaaactttt gaacataaac ctgaaaaata taccgagaag gtaaatcata
     3361 tttttgaagt actcggtatc tcactttttg aatgctacga catgaccgag aagctttata
     3421 atgaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag agttcagatg
     3481 aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat gagtcgctcg
     3541 cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct acctgatttt
     3601 aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat atatgcgatg
     3661 gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct cgaagtagca
     3721 aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga agatgtggat
     3781 ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta tgtagcagac
     3841 tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa tttagttttt
     3901 acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta taacgaaaaa
     3961 ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg gacagctatg
     4021 tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct aaatacactg
     4081 ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga gaacatgatg
     4141 gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa
     4201 aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag
     4261 aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga attgtgaccc
     4321 ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta
     4381 catattttgt aagtgatata gaacctagaa ccgtttatat ctaatgatga ctggcttaat
     4441 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     4501 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     4561 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     4621 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     4681 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     4741 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     4801 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     4861 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     4921 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     4981 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     5041 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     5101 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     5161 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     5221 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     5281 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     5341 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     5401 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     5461 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     5521 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     5581 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     5641 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     5701 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     5761 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     5821 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     5881 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     5941 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     6001 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     6061 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     6121 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     6181 tccctatacg aatagcttag acagccgatt agccgaattg gattacttac tgaataacga
     6241 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     6301 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     6361 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     6421 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     6481 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     6541 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     6601 tgtctaaaaa gctttaacta caagcttttt agacatctaa tctttt
//
LOCUS       DACBZN010000124         6639 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.124, whole genome shotgun sequence.
ACCESSION   DACBZN010000124 DACBZN010000000
VERSION     DACBZN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.124"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(1273..1938)
                     /locus_tag="IVD64_002525"
     CDS             complement(1273..1938)
                     /locus_tag="IVD64_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6149735.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IVD64_002526"
     CDS             2064..4100
                     /locus_tag="IVD64_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP6149736.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IVD64_002527"
     CDS             4104..4742
                     /locus_tag="IVD64_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149737.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IVD64_002528"
     CDS             4770..5198
                     /locus_tag="IVD64_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149738.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IVD64_002529"
     CDS             5655..6566
                     /locus_tag="IVD64_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6149739.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADNGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2286 a         1005 c         1220 g         2128 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataatggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaa
//
LOCUS       DACBZN010000125         6460 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.125, whole genome shotgun sequence.
ACCESSION   DACBZN010000125 DACBZN010000000
VERSION     DACBZN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.125"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(105..560)
                     /locus_tag="IVD64_002530"
     CDS             complement(105..560)
                     /locus_tag="IVD64_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149740.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IVD64_002531"
     CDS             complement(714..1922)
                     /locus_tag="IVD64_002531"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP6149741.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IVD64_002532"
     CDS             complement(2034..2366)
                     /locus_tag="IVD64_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6149742.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IVD64_002533"
     CDS             2514..3389
                     /locus_tag="IVD64_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6149743.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IVD64_002534"
     CDS             complement(3471..4052)
                     /locus_tag="IVD64_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP6149744.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IVD64_002535"
     CDS             complement(4058..5317)
                     /locus_tag="IVD64_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6149745.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IVD64_002536"
     CDS             complement(5319..6365)
                     /locus_tag="IVD64_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149746.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLIT
                     MTVFQQIVSEIRGN"
BASE COUNT         1797 a         1337 c         1103 g         2223 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcacttgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 gtaatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttatc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata
//
LOCUS       DACBZN010000126         6286 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.127, whole genome shotgun sequence.
ACCESSION   DACBZN010000126 DACBZN010000000
VERSION     DACBZN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.127"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..177
                     /locus_tag="IVD64_002537"
     CDS             <1..177
                     /locus_tag="IVD64_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149747.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IVD64_002538"
     CDS             190..690
                     /locus_tag="IVD64_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6149748.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IVD64_002539"
     CDS             787..2634
                     /locus_tag="IVD64_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP6149749.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IVD64_002540"
     CDS             2637..3533
                     /locus_tag="IVD64_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6149750.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IVD64_002541"
     CDS             3583..3972
                     /locus_tag="IVD64_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6149751.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6286
                     /locus_tag="IVD64_002542"
     CDS             3959..>6286
                     /locus_tag="IVD64_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6149752.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1887 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagaggcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaac
//
LOCUS       DACBZN010000127         6265 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.128, whole genome shotgun sequence.
ACCESSION   DACBZN010000127 DACBZN010000000
VERSION     DACBZN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.128"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(95..667)
                     /locus_tag="IVD64_002543"
     CDS             complement(95..667)
                     /locus_tag="IVD64_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6149753.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IVD64_002544"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IVD64_002544"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6149754.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IVD64_002545"
     CDS             complement(1352..1867)
                     /locus_tag="IVD64_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP6149755.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IVD64_002546"
     CDS             complement(1869..2153)
                     /locus_tag="IVD64_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6149756.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IVD64_002547"
     CDS             complement(2187..3515)
                     /locus_tag="IVD64_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6149757.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IVD64_002548"
     CDS             complement(3529..3984)
                     /locus_tag="IVD64_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6149758.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..5933)
                     /locus_tag="IVD64_002549"
     CDS             complement(3987..5933)
                     /locus_tag="IVD64_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6149759.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2060 a         1205 c          875 g         2125 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taattttaat aaatttacat gtctttcaat caaacttgtt
     5941 cacctcttaa cgctagcatt tgttacctct tttcattatt tattctagca ctggaacccc
     6001 cttccctcaa ttacatctat gtcaataata cttgtggaag tttgttgtaa aggaaatcca
     6061 aataatctta aaaaatgaga aacaaaaaag gagaaatcct taaaagagct aagatattcc
     6121 aatagaaaat actccttatt atggaagtaa tatgaaatat tacaattgaa aaataaaaac
     6181 aatataatgg cgtcactaaa attcgtattt ttttattata atataactta attacaaatg
     6241 ctcatctatg aacaattgag tccta
//
LOCUS       DACBZN010000128         6181 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.129, whole genome shotgun sequence.
ACCESSION   DACBZN010000128 DACBZN010000000
VERSION     DACBZN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.129"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(38..1018)
                     /locus_tag="IVD64_002550"
     CDS             complement(38..1018)
                     /locus_tag="IVD64_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6149760.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1042..2526)
                     /gene="galT"
                     /locus_tag="IVD64_002551"
     CDS             complement(1042..2526)
                     /gene="galT"
                     /locus_tag="IVD64_002551"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP6149761.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2538..3527)
                     /gene="galE"
                     /locus_tag="IVD64_002552"
     CDS             complement(2538..3527)
                     /gene="galE"
                     /locus_tag="IVD64_002552"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP6149762.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3775..3942
                     /locus_tag="IVD64_002553"
     CDS             3775..3942
                     /locus_tag="IVD64_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP6149763.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(4004..5815)
                     /locus_tag="IVD64_002554"
     CDS             complement(4004..5815)
                     /locus_tag="IVD64_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP6149764.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5812..6177)
                     /locus_tag="IVD64_002555"
     CDS             complement(5812..6177)
                     /locus_tag="IVD64_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP6149765.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
BASE COUNT         1691 a         1308 c         1108 g         2074 t
ORIGIN      
        1 gcggtatcac tccattttcc cacagctttt ccatctttta tttcgttgat tctcgaataa
       61 tcaactctgt ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa
      121 gaatcgtctc cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagcg
      181 agggcgttac atatttcgct acactgacat cattgaagcc catcacagaa atatcttctg
      241 gtaccactaa acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag
      301 ctgcaaaaag cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat
      361 atcctgactc gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg
      421 ccaaaacttg ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg
      481 tatattctgt tccagcaagc atagcgatat gttgcgcttt atgggagaca aagtgttcaa
      541 tgactttttg aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc
      601 cgtcagaatc taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt
      661 cgtcgaattt tcctagtgcg atcgttccat ctgccggaag atcactaatg gaatcccacg
      721 tttctttcaa taaagtaacg tttgcttctt ctgccttttt ttcgattcct aggcgaatag
      781 ataaataata aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg
      841 tttgcttagt atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt
      901 caaaaatttt ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc
      961 ttgaaacagt agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt
     1021 gtctctcctc cttttttacg tttataaaat cgcagtaaat cgttcaaacg ctgcctgtcc
     1081 tttttcatct cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac
     1141 agatttttgt acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa
     1201 ttgttctgcc cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg
     1261 ctctacttct actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc
     1321 gatcaaccca atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc
     1381 atcaggatgt tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt
     1441 gtcccttctt cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc
     1501 tctgatagat actgtttcat ccgaatatgc tttccatttt tccaatactg agttagctgc
     1561 ttcaataagc aagtttttct ctgttgattg caaacggatc acagacatcg gccatttcac
     1621 gactcctgcg atgacttgcg gataatcttc tagtttgata agtttttcta tcggtgcttt
     1681 tgccatagga aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc
     1741 aacaattggt aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg
     1801 aagcagtcta gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat
     1861 gatcgaatgt tcattgtaat aagcataagg ggaatattgg aatccccagc tttcaccgtc
     1921 tagattcatt cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata
     1981 tccttcgttt tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc
     2041 tgctgcgatt tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc
     2101 accatactcc gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta
     2161 gtcatttttc ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata
     2221 gtgctgtgca aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg
     2281 cgcttcaaaa atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt
     2341 agcgacaagc tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct
     2401 ttctagagca tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat
     2461 ttccatccat ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat
     2521 tgacatatct cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat
     2581 gctgttttga taatcgcttt gatatctgtg tattcaggtt tccagcccag aatctctctc
     2641 gctttttcac ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct
     2701 gctggaattt cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat
     2761 cctttgctgc tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa
     2821 gctaaaatat gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca
     2881 tctggtgtat cataatcgtc tccataaaca gccaaggctt ttctttgacc aagagctact
     2941 tgtaaaatga ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct
     3001 gcttttgctc cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg
     3061 tcacaccact tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga
     3121 ttagctggtg tatcttctgt gattggagac tctttcggct ctccataagt tgctgcagta
     3181 gaagagaata cgattttatt cacgttgaac tcgtgcatga cttcaagtaa gatttgcata
     3241 ccgtatacgt tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag
     3301 cttgcagcaa aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat
     3361 tctttatccc ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga
     3421 cctgtgagca aattatctac cacagctact tgatacccct tttgaactag ctggtctaca
     3481 gcgtgcgaac cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct
     3541 ttgattagta aaattgtgtt tgttgattct atataattta aaaaacgaag tttactttta
     3601 tggtgaaaac aaacaaagta aaatttcgta aattcatatg gtaaatttat aataaaacat
     3661 ttagtaaaca ttttcaagga ttttttgcaa aggtttacaa acaaacgtaa aactagcctt
     3721 tttttctctt ttgttccata atagatataa caaataataa atgaggtgga ttcaatgaaa
     3781 aaatttgtat ctggaatatt agtaggaagt ctggcaacag ccgctgccgt tgctggtttg
     3841 gttgcttctg tgaaaaaaac agtgatcgat ccaattgacg aaaaagaagc aatgatcgaa
     3901 gaaaaccgta aaaaagcaat gcgtaaacgc gtttctcgtt aataattatt taaaaatacg
     3961 catgaagact ttctaaaaaa cagataagtc atcatgcgta tttttatttg tcagtcaata
     4021 taagttgtgc tattttatct gcctgtctca gattccatgg atctgtataa ggtctagcta
     4081 ctgctgcagt tagccaatcc acaccttcaa caggtattcc atagcaataa aagtcagact
     4141 ggatttcccc tgaagcatca atcacgcgat tcgtacctcg ctcaacatct atagcttctg
     4201 aatgataact tcctgtatta tctgaaaatg aaaaagggcg aacaagtttg ttttccttca
     4261 aattttttat gacaggatta cttgaacgag aaaagcttgt ttgtggcaat cgagcttcaa
     4321 tcaaatagcg actgctgatt tctttatctt gtagagaaat aacatagcgg ttgtgctttt
     4381 gctgaatatc aaatggcggt tcgatcaaat gaaaaatacc cgcatctatc agtgctgcta
     4441 attctttcgt ccgaatgatt ggcggaccga tcgttaaaaa gctatttaga tgagtaaacc
     4501 atccccatag tatttctttg tattccttag cagaaaaaac gtgccattca atagcctgcc
     4561 ggattggatc acgccagtct tttaatgcat caaatgttga agcaaatgct cctgtgcaat
     4621 tgcctttttc cgcttctttt atctgccctt caataaatat tcggctagct tctgaaaagg
     4681 gctcattctg ataggcaaga ggagtttcta aatacgacca atcccatcta taagggatta
     4741 gtgctggatg ctttccgaga cactcctctg aagagaggga gagaaaatca tgctcaaaat
     4801 ttttctttgg aatcggcaag tgatattctt cgatgatttt cttgtaataa aacaactcaa
     4861 cgtctttttt cagatagtca aaaaatgttt cgccagtcaa aaatcccgac tgatgccatt
     4921 gctccaggtt ttcttttgtt agtaaccgtg gccatgccat tgctccgcct tgcttttgat
     4981 ttcttcctcg agggtaataa gggagccccc ttcttgatcc actgtataca atcggttcct
     5041 tgcctgatgg atgatagacc agttttccct tttcgttctc gaagcgccct cctcttgata
     5101 ctgtaaacaa tccgacataa tcaaaaaaac ttaagcctag acctcttaaa aagacatttt
     5161 ctttaggcgg tatcttcgaa agatctacat ctgcaggatt agaaggaagc tgatacagta
     5221 aaacattttt ctttgcgtag tctacccaca ctctttcttc ttctgtctca tcattttccc
     5281 agtgaccgct tgctaaaaca agctgatcga tagaatagct ttgctgatct gtctgtaaaa
     5341 gataaccatc ttttcttttg cttacttttt ttactaaaga ataaacaaac tgaatagaag
     5401 ctggaaattc ttcttgcaac tttgagaaaa accagcgttg atagagtccg tataaacatc
     5461 gagtagattg ctgatctttt tccagacgat tgatttcttc tataattagt tgcttatttt
     5521 tggttaccgt ctgttcgata taatctgtag caggtccttt actccattga aacaagttgg
     5581 gtccctgtgt taccaatcct ccactggaca atgtttcatc tgtaaaaagc gtcacttgtt
     5641 gagaaacaga attcattaat aactccggtg gctgattcaa ccgccaaact cgacctccag
     5701 gaccattggg atcgaaaaga aaaagttcaa gagaatgtct gtcctgatta ttatggagaa
     5761 tcctttctgc tacacttaat cctctaggtc cggcaccaat gatccctatt ttcatttgca
     5821 tgcctccatt tcgataaatt ttttcaataa aacagcatgg ttctgatgtt cattttttgc
     5881 accataaaca aaagtaatga ctggatgttc tttcataagt tgactgagtt taaaaatagc
     5941 ttgcttcttt tcttcttcga aaatcaattc ttgctggtat ttctttgcaa atgaagcaaa
     6001 tcgttctggt acatggtcaa aagttttgcg tgcttcatta ctaggtgcaa tttctttcaa
     6061 ccacagatct aactgcgctt tttcttttga aattcctcgt ggccagaatc tatccacgag
     6121 tattctatag ccatcctctt tttcagcatc aaggtaaaca cgttttaatt tgatcatagt
     6181 t
//
LOCUS       DACBZN010000129         6124 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.130, whole genome shotgun sequence.
ACCESSION   DACBZN010000129 DACBZN010000000
VERSION     DACBZN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.130"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..189
                     /locus_tag="IVD64_002556"
     CDS             <1..189
                     /locus_tag="IVD64_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6149766.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="IVD64_002557"
     CDS             complement(275..1369)
                     /locus_tag="IVD64_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6149767.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="IVD64_002558"
     CDS             1558..2319
                     /locus_tag="IVD64_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6149768.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IVD64_002559"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IVD64_002559"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6149769.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="IVD64_002560"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="IVD64_002560"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP6149770.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..4972)
                     /locus_tag="IVD64_002561"
     CDS             complement(3959..4972)
                     /locus_tag="IVD64_002561"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP6149771.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5137..6057
                     /locus_tag="IVD64_002562"
     CDS             5137..6057
                     /locus_tag="IVD64_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP6149772.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1880 a         1214 c         1094 g         1936 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtc
//
LOCUS       DACBZN010000130         5980 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.131, whole genome shotgun sequence.
ACCESSION   DACBZN010000130 DACBZN010000000
VERSION     DACBZN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.131"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            148..585
                     /locus_tag="IVD64_002563"
     CDS             148..585
                     /locus_tag="IVD64_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149773.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            680..1564
                     /locus_tag="IVD64_002564"
     CDS             680..1564
                     /locus_tag="IVD64_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6149774.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1788..4955
                     /locus_tag="IVD64_002565"
     CDS             1788..4955
                     /locus_tag="IVD64_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149775.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4975..5520
                     /locus_tag="IVD64_002566"
     CDS             4975..5520
                     /locus_tag="IVD64_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP6149776.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5548..>5980
                     /locus_tag="IVD64_002567"
     CDS             5548..>5980
                     /locus_tag="IVD64_002567"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP6149777.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFIL"
BASE COUNT         2008 a         1013 c         1315 g         1644 t
ORIGIN      
        1 taaatcaaca gtagtttaat tactacttga tttagttctt tttattttaa aaattttttt
       61 tacttagaat ccagcgaccc tagatatttt tctgtataat cttaaataaa gaaaacaata
      121 tcaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct tttatttcta
      181 gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg aagtacaatg
      241 gatcatttgt atataaaata tattcatcaa ttttccgttt tggtgttagg cacactaatt
      301 atagcgggga ttgtcgcatt tattattcaa ggccgtgaca tattaacagt acatcaagag
      361 tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa caaaaagtgg
      421 aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc taagattttt
      481 actttattag gtatacaatg cctatcattt tcttttattt cctatatcat tgttttaatt
      541 ttcctcagtt tcatagtatg gatgttttat cttgtcaaaa aataaatgaa tgtttcatga
      601 acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta aagaaatcaa
      661 tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg
      721 tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg tgtttgatga
      781 agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc
      841 tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca acgaaggaat
      901 cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat tgggtacggt
      961 gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga tcaatcattt
     1021 ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact attttatgaa
     1081 tgtcgtcgca attggctcta tccctgaatc aatcaatgat gtagatgcgg agaagaagac
     1141 gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt ccacacaatc
     1201 ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca gtaccttgct
     1261 gataggacta actaactcag tcggcggttt tgaaacatta cttccaaatg caaaagtgga
     1321 tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata cgcttaaaac
     1381 attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct accttacttg
     1441 tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg ttgatggaga
     1501 tgaaggggat aaattacctg tgacaatcaa agtattgccc tcacatttac aggtttattg
     1561 ttaaaatgga ggttgtgacc gaggagttgg cgggagaaca ccgcttgttc gggataaggg
     1621 actgtgacaa gacttttgtc acagcctttt ttttgtacaa aaaagaaatt tacacctaat
     1681 agaaaaattt ttccataaaa atacatattt taaatatgaa agtacaattg gaaatagatt
     1741 aaaacgataa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatgtg
     1801 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
     1861 ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
     1921 ctactttttc agaagaatcg attacttctg aaactacaga atctcaaagt gaggcttcta
     1981 ctaataaaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
     2041 ctgatagcag tgaagcggca ctggcaagag ctgtttcagc ggatgggtac agcgaagctg
     2101 ccacaacaga agaactggcg cagcttctcg cagacgaatc agtaactaag atccgactga
     2161 ttcagccttt gactttagac agggaattag agatcaaacg agatatcgtt attgattttg
     2221 gtggattcgc tcacaatttc ggcacacacc atatttatat caatgagata cctcagtcga
     2281 tcgagttcca gaattttaaa ggaacagctg cacatccagg tggattgaca ccaaatatcg
     2341 atggaaatgc cattatcatt gcttatatga ccgatttttg gggaagcaga tatcatttta
     2401 cgggagaaat aaaatttacc gggacacttg atttgtatga tgggtccaaa ttaggactga
     2461 tttacgctcc tcgtgcaact gtcacattag atggtgtttc cggtgtgttg gatgtccaac
     2521 cagtgaagga aggactgaat gccgctccgg gtaaagccta tttttgtaga agctatcaat
     2581 tgaatgtgat caatggtagt caactttatg gaccgtatct agggaaattc tacggttttc
     2641 tagattcagg agatgagact ggtagtggaa aaacagcgcc aggaatccat atcttaagcg
     2701 gttcaaaggt ctctcttgat tatgatcggg cagatggcgt tagcgatgaa ggagaggcag
     2761 tcgatacgct acctggtaat gtcatgtttg aagtcagtgg ttcgggaagc gaattttcgg
     2821 taaatacaaa gatcaatatt accaatgaca acaatcgcgg aatcatccaa atgagaggtt
     2881 caggatcgaa agtcactgta tcgaatggtg ggaaaatcac catcaataca gcaactacgg
     2941 gcggatttcg cttgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
     3001 cggtcaaaat ggctggagat gacgatcaac ccgggaacaa tgggatgcgt tttgtaggaa
     3061 aaggcctttc tttgatcgta gaaggagccg gatcagttat cgacatcgat aagcagagtg
     3121 ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa gctgacagta accaatggag
     3181 gctcattgag ggtaagaaat acaggtgatg gcaaatctta cgtgaacaga ctcaatcaag
     3241 gaaatcaagc gatccaattt tatgacgctt ctggttttct agaaagtcca ggttcagctg
     3301 attttacagt cactggagaa aaatcaaata tcgatatacg cgcagattcc ggagcaactg
     3361 tagatacaac tggagatatt gatttaaatt ttacggcagg tgaaaaaact tatttagtca
     3421 tggtcgggaa aacaggggaa gataagaccg gcatcatcag tggggatgtg ttgaatgtgg
     3481 acttagaatc tcctacctat tttgatttcc aaaacaaacg gattggaagc gagaccaatg
     3541 gcataggcgg ctgggtattc caaggaaatg gagattcttc tttaaagatc gcccattcag
     3601 aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa ctttgaaaaa gacccggatt
     3661 atttttctga tatgacagac ttatcgatca atgggaccga cttaggcaat ttcgtatcaa
     3721 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
     3781 atcgaatcag tgccaataat cagctcccga tgattgaatc cttaagagtg ccaacgaatg
     3841 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
     3901 atgcttggac aggcgaagta gaagtgactt tacgtattac ttacgcagat acgacgaaat
     3961 catctgtaga aattactgct ctcacgcaag gacaaacaga tgaaaatgaa ggggcaggat
     4021 ataacccatg gggagaaggc gaacaaggcg gcttgttcga agttcaagta cccaatgatg
     4081 agagattaaa aacggaggat caagtgcaag ttgttgctgc taaaaaagta agaggtggcc
     4141 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgaacctg
     4201 ctaatttaag taccacattg atcaatgcag cttctcgcac gatcacaggt acaggtgaag
     4261 caggagcctt ggtttcattg aaaaaaaatc aagctgtttt gtcagaaaca actattgatg
     4321 atacagggga atttgttctt tctatcccag aaaatcaact ttttgcagga gatgtattag
     4381 aaattgtatt aaacgaccaa gcaggagaag ctactgctaa gggtgtgcag aacaagccaa
     4441 gtaccaacga tgaaattggg aatcacaatc caagtgatac gccagttgtt tatcatgatg
     4501 ccccgccttt tgcacctgct acgaagatcg tagtagaagg tggtctctca tttcttgccc
     4561 cttccaaact cgattttggt cagatcaaag caactggttt gacacaagaa gcatttggcc
     4621 aaatggatat acaggaaaaa ttatggattt atgatcagcg agaaacgaaa aatccgtgga
     4681 tcttaacgat aaaattatct cagcctttca caaatgatgc aggattttct atgcaggatg
     4741 tcctatattt caagaaagga gaggagtatc aattagtgaa tgaagaagaa ctggaaattg
     4801 tcagaggaca aaattcaacg aatgtgcaag cagattattc agaatattta aataacggtc
     4861 gggcgtttaa acttgtctta accaaagaaa atcagatagc tggtaattac tcagcagaga
     4921 taacatggac attagcagat gcgccgacag aataaagatc aaggaggatg attgatgaat
     4981 cgaatatgga ataaaagtca tcgaaacaac tgctctttac ttatgatcct tctccttgga
     5041 ttcatccttt ggcagacacc tgtccatgcc tccgaattca attttgcggt agattttgaa
     5101 ttaccggcta atcagattga taagaacaaa acatatataa atgtaaggat ggagcctggg
     5161 aaaacacagg aaatccccta cacggtcttt aaccgaacag ataaatcagt aacagttgag
     5221 acattgatcc actccgctac gacgaattca aacggtgtga tcgaatatgg agaatcaaaa
     5281 agtaaatttg atgaaacatt aagttatgat atgaaaaact tagcaaaaac agtgggagaa
     5341 ataactgttc ctcctcaaag tgcggtcact gagacacttt cactgaaagc gccagaaaaa
     5401 gagctggctg gtgtggtcgc aggagggatc acgtttcaag aaaaaaaaga gaacgtttct
     5461 aacaaaagta cagttggtgt cgtcaatcag ttttctcaag tgaaagcaat cttgatttga
     5521 aatagcgaaa aagagtcttc cccagatttg aaattaaaaa aagcttatgc ggatcaaagc
     5581 gatggaagaa atgtgattaa ggcgaatctt caaaatccaa atgcttctta tatgttcggc
     5641 ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag agccttacct ttcaataaaa
     5701 aaagaaaaaa tagatgtagc acccaataca aattttgatt tacttgtccc attagaagga
     5761 aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg gtgcttggaa agaaaaacgc
     5821 tggaatctcc ataccgattt tgaaataact gatgatgcag cggaccaatt gaatgaaaag
     5881 gatattgatt taaaaaatca gccagaaaca tttgactggc gatggctgat acttgttgga
     5941 gtcattctta ttggcttatt tgctggatta tttattttaa
//
LOCUS       DACBZN010000131         5909 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.132, whole genome shotgun sequence.
ACCESSION   DACBZN010000131 DACBZN010000000
VERSION     DACBZN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.132"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..574)
                     /locus_tag="IVD64_002568"
     CDS             complement(<1..574)
                     /locus_tag="IVD64_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP6149778.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(540..1727)
                     /locus_tag="IVD64_002569"
     CDS             complement(540..1727)
                     /locus_tag="IVD64_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6149779.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(1791..3518)
                     /locus_tag="IVD64_002570"
     CDS             complement(1791..3518)
                     /locus_tag="IVD64_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6149780.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(3515..3967)
                     /locus_tag="IVD64_002571"
     CDS             complement(3515..3967)
                     /locus_tag="IVD64_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP6149781.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(4078..4458)
                     /locus_tag="IVD64_002572"
     CDS             complement(4078..4458)
                     /locus_tag="IVD64_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6149782.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(4455..4856)
                     /locus_tag="IVD64_002573"
     CDS             complement(4455..4856)
                     /locus_tag="IVD64_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149783.1"
                     /translation="MAIIKIRTSITGTEYWDSEKKKTVAVPKGQEPKFDTSEQTDMTV
                     VAKSIVGDGVLFKSGEEIHLSGEELDSDGNTAADFDGEKVTDDQFDGTTDELEDMTAK
                     ELRAYAKKHGIDIPGAVRAKGDIINLIREAE"
     gene            complement(4837..5223)
                     /locus_tag="IVD64_002574"
     CDS             complement(4837..5223)
                     /locus_tag="IVD64_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149784.1"
                     /translation="MNDFHEAVLTFDVPTGMGQVYKKAIEDDNSRRWIKNEIKDADGN
                     IVISDIKPSWNGNHCSVSIDNSLTIEKYSGAIIDRTERKTHLTIALISRSLPNLKEQV
                     EWYERMGAKVISTNYKGENQNGNNQN"
     gene            complement(5528..5671)
                     /locus_tag="IVD64_002575"
     CDS             complement(5528..5671)
                     /locus_tag="IVD64_002575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149785.1"
                     /translation="MRIGNVYLISKVPYQKLLYSCKVRFIASDLRNLRKQFILSDADE
                     LAD"
BASE COUNT         1645 a         1275 c          939 g         2050 t
ORIGIN      
        1 acgcttctag cgcttgatta atttttttga ttaatttttt tctttcttcg acgttttttt
       61 ctaattcatc acgacttctt aatgcagctt ctgtatcttc gtatgctggc aaaggcacaa
      121 tagaaacctc gtataattcc acttcatgga ttgttcgaag cattggttca gaattgtagt
      181 cccacgtttc ttcagtcggc acaaaaccaa aactacattg gttgatgtct cctcgttcca
      241 tagatttaac taagtccctt gctaccgttg tgtccggtaa atcaacttcg aatttcaaac
      301 cacgttcatc ttcttcaagg cgaagggttc cgcttttagt acgtcctagc acattggacc
      361 aatcgtgatt gaacaaacaa cgaacatccg aattactaat tgcctttgag aatgcaccag
      421 gaacaatcac ttcgcttaag tcatcccata aaagtgtttg actgttgaat acagcagcgt
      481 agccgctaat ggtcctcgta tgagtttctt catcggaacg tgttgaaaga ttggtgatgt
      541 caatcgtgcg aatttccttc ttcttcattt ccatcacctc ctttcaagtc ttgatcattc
      601 gttggtaagg aatcatctgt tgcatttttc tggccaatcc tagataagtc atttgaaata
      661 tagatagctt gtgtttctgg agtattctgt ttaggaaaac caagcatttc tgccacgtta
      721 tctggacttg taatcccggt acgaacgatg ttgtagccaa tatttgtttt tgttgagtaa
      781 ggaacaaaat ccaaaatatt aattttccat tccactctat agccagaatt aggcataaaa
      841 aaaagagcgg tgtaatgttc gctcttgttt ttcaatattg gtttgattgc tttgttgtgc
      901 agatacatca tcgctttttc aatatctgtc ttcatcagcg attgatacgt atcaacattg
      961 attcctagaa attttcctaa gtcttttttg taaacaccta aataattcaa aattgccgcg
     1021 tcatcaacag gactttttaa tgtctcgatg gaatatcctt ttcccaaagg aatcatctta
     1081 acagaatgat cactctcatt ttgcgttcct tccagttgat ccaatatagc tttgacaatt
     1141 tttgtttggg cgctattatt tggattgatg tgggcgtcta gtttaagcat gaacgcaagt
     1201 aaaccgcctt tagtatattt gtccgtcaaa actttttcag cgctcagaac gccttctaga
     1261 gtgtttcttg caagatcaat tattccagca ccttttaatg aatcagttcc gatgttctta
     1321 atgtgtcgaa tcatttgacc aggtattttt tgaccattca tttcaaattc ttcttgaagt
     1381 cgttcattga ttttagttgt tacaccgtac gccaaatgaa gctgatccct atccgttaat
     1441 gggaatgtct caccattgat caataaagta tttgtttcca atttggtaaa ttcgaatcct
     1501 gtcaaataat cattgggact cttcaaaata tttagtaagt gatgatcttt gacttcgttg
     1561 ccgtcgggac caataacaat tggtgtggcc aatgccacct gattagatat gtcttgaact
     1621 aattcataaa catcagaaga ttccatgata gatgaatccg tcacatatct ttgaccataa
     1681 cgtgtgtagt gtccaaaaat atcctcgata tatccacgtt tttccataaa agaatagact
     1741 gcattcgata accgatcacg taatttcaat ttttttcacc gcctttctat ttatctatag
     1801 atggaactta ggtaatcatc taactcatct gaatcaatat ctgtcatttg attcatcgtt
     1861 tccttatgac cacacaaaaa cgccacgaac ccatcaatct ttttctttga ttgacgttta
     1921 cttggcgctt tttgtccatt gatgttagta attgctacaa cgttcaaagt gcaataaagg
     1981 aataatggat tatcaaattg aattcgtttc tcataaaaca accgttcgac atcgtcaaaa
     2041 ggagcattca atactttagg atattgagca acttcaacgc attccaatcc taagttttct
     2101 aatttctcaa caagtttgtc actcatcgct ggatcataat tcacttgctg aatatcatat
     2161 aaattcatgc agtcctctat gtactgcaaa atctgatctt gatcaatcat ttttccgtcg
     2221 caaaattcaa cgaaaccttg ttcagccaaa tcactgtaag gaacattatc ttctttttct
     2281 cgaaactcta aatcttcatt cggaataaaa taaagttgct ttactttaag aaccgcttta
     2341 ccatcttcat cccatgttgg gaagtttaaa gatacacagg tcaaatctcg tgtacgtgat
     2401 aaatctaaac cgatataaca tggttcgcca cttaaatcac caagttcttg tgtggtaact
     2461 aaacatggct ctacttgatc ctgttcaaag aaattatccg cgccgttcac aaatacatcc
     2521 aagtgtttcg ttagaaattc agctttggaa tgagctgacc gttgcgctgt tttaaatgcc
     2581 gattctaaag cagataaatc gactgatatt ccccagttag gattgcacat ttcccagact
     2641 tttctatccg tccaatcata atttttattt ggctcataga ttagaacaaa gtttgaatca
     2701 ttatcatcac gtttcaacac ttcttttgct tcgcgatata cacgcattcc aacagacgac
     2761 gaacctttcc cagcagttga gatattaaac atcaatggct gtggtaaaga tatctgagca
     2821 gacttaaaat tgtcatactg ttccattttc tcctgtttat gcaattcatc gttcaataca
     2881 aaatatggat tggatccctc gatattatca atattcttgg tttgaacaat aaatttattt
     2941 gagtaagcca tatcttcatg aatatagtca taagtaatac tagaaacagt tccctttggc
     3001 cccctaaata ttttagttcc ttcaagtagg attggattgt ttaagattgt aactgcaaaa
     3061 ggtttggcag catattgcgc ttgagcaaaa tcagatgcgc aagcatagca atcaactgat
     3121 aacgcccctt ctccgtacat tgcatatcct aatgaaccga cagcaatcaa tgttttacca
     3181 ttcttttttg gaatctggac ataagcctca cgagtaacgc gtacaatttg gcctttttca
     3241 ttttctttaa cccaaccata aatccaagaa taaataaatt tttcccaggg ttctaaaaga
     3301 aacggttttc ccaccatttc tccttttgtg tgaacaataa atgattctac ccaatccatc
     3361 atttcattcg cgcgatctac atcaaaccaa atatcttttc tttttttcca ctgataccag
     3421 cgatctattg ctaaacgaac ggtcttagga tacttcttag gtttctttct tacttctttt
     3481 gcaaataaat cagcataatt tacaccaggt tcaatcattt ttcagcacct gccttcttac
     3541 gccatttatt tctgtgcgct gctagttcat ctactggctt tacttctggg cgtgtaattt
     3601 cttcatcttt tcttgcggtc gatcctccag caattagtct accagttttt gctttgtttg
     3661 ttaatcctaa taaatctaga gctttcatct tcttatctgc ccaagtttct acttgttgtg
     3721 ccaatggatg ttttgattga ttagtagctc ctgatttatt cgtgaatttt tgcgtctccg
     3781 gaaatccttt ttccttccac aataaatatt tgtgttggta aatttcaaaa atatccaagt
     3841 atgattcgat taatggatca agagtgatgg tgtacaaatc agacaaattc attatttcta
     3901 agatacgagc tttttcagca cttacttttt catcaataat cgctttacgt tgcgctttag
     3961 tcgtcatttt tgtatacacc cccctttgtt ttttgaaaaa tttgacctaa cgatgcgcgt
     4021 gactccccgc taccctatct ccccacgcga aaaaattttg aaaatggata gggggggtta
     4081 attaaaatac gaaggaaaaa cttttttgtc ttctgtttcg ttttcaacaa ttggatgaca
     4141 ttttgaacat aaaagcatga gattgtttgg atcaagctta agcagttcat tgtctttgat
     4201 tggtacaatg tgatggacgt gtgccctctt gccaaagatg aactgaccac atcgctgaca
     4261 gtggccacct tctctttcgt aaataaattg gcgcatatct ttccatgcct gtgttcgata
     4321 gaatggtttg ttctcatgat gataaacaga ctttgcttgc tgcttcttct tgcgtgatct
     4381 gcttgatctc ttgtgttcag tacagtagat accctttgct atcttgttcg tgcatccgtc
     4441 aaactgacag tacttcattc tgcttcacgg ataagattga tgatgtctcc ttttgcacgg
     4501 acagcaccag gaatatcaat gccatgtttc ttagcatatg cacgtaattc ttttgcagtc
     4561 atatcttcca actcatctgt tgttccatcg aactgatcat cggtaacttt ttctccatca
     4621 aagtcagcag cggtgtttcc atcgctatct aattcttctc ccgataaatg aatttcttca
     4681 ccactcttga acaagacacc atcaccaacg attgacttgg caactacagt catatcagtc
     4741 tgttcacttg tatcaaattt aggttcttga cctttcggaa ctgcaacagt ctttttcttt
     4801 tctgaatccc aatactctgt tcctgtgatg gatgttctaa ttttgattat tgccattttg
     4861 attctctcct ttgtaatttg tactgattac ttttgcgccc atccgctcat accattcaac
     4921 ctgttctttt aaattcggta agctcctaga aatcaatgct atggttaggt gagttttgcg
     4981 ttctgttcta tcaattattg ctccggaata tttttcaata gtcaggctat tatcaatact
     5041 gacactacag tggttgccat tccagcttgg cttgatatcg ctaatcacaa tatttccatc
     5101 ggcatctttt atttcatttt taatccaacg tctactgttg tcatcttcaa tcgctttttt
     5161 ataaacttgc cccatacccg ttggaacatc aaaagtaagt acagcctcat gaaaatcatt
     5221 catagataaa actcctttca aaataaaaag accactcaaa gagtgatcta atatgtaata
     5281 gcaacctaca cacagacaag tctaatactt cctgcgcctc accactgcca catccctcgg
     5341 ttgctaacga atactgaaac agaataccgt ctctgttcat gaccaacgcg acagtacaaa
     5401 gctttgtcgc aagcaggtta tcgtattcct aataacccca actaatgttt ctctcctaac
     5461 ctacacctga gagtggcgca cgtctgcgct agtattttat gccttctaac tgctagtagc
     5521 atcccgacta gtcagccaat tcatcggcat ccgataagat gaattgtttc cgtaggttcc
     5581 ttaagtcact ggcaatgaat cgaaccttgc atgagtacaa taatttttga taagggactt
     5641 ttgatatcag atatacgttt ccgatacgca tgtactctct ccctcaatgc gtctaccctt
     5701 tccgccacaa tgacataatg acaatagaca gcaacggatg atagataata agaacaattt
     5761 agaaggagtt ggaattcaca tccttattct taatatttcc gtcgctatct atcgaagctt
     5821 aattgtgaaa caataataaa acgatgttcc ttttattatt attttgtctc agacctatca
     5881 ctaatctttc gacactatca taatacaac
//
LOCUS       DACBZN010000132         5898 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.133, whole genome shotgun sequence.
ACCESSION   DACBZN010000132 DACBZN010000000
VERSION     DACBZN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.133"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(42..680)
                     /gene="plsY"
                     /locus_tag="IVD64_002576"
     CDS             complement(42..680)
                     /gene="plsY"
                     /locus_tag="IVD64_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP6149786.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            807..1241
                     /locus_tag="IVD64_002577"
     CDS             807..1241
                     /locus_tag="IVD64_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP6149787.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1407..3452
                     /gene="parE"
                     /locus_tag="IVD64_002578"
     CDS             1407..3452
                     /gene="parE"
                     /locus_tag="IVD64_002578"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP6149788.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3464..>5898
                     /gene="parC"
                     /locus_tag="IVD64_002579"
     CDS             3464..>5898
                     /gene="parC"
                     /locus_tag="IVD64_002579"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP6149789.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPE"
BASE COUNT         2100 a         1017 c         1322 g         1459 t
ORIGIN      
        1 tttgtcacac tcccgtttta tctctttgga atgaaaaagt atcaggattt tttcttgctg
       61 ctattcaaac caaacgggat tcgactttca gtatggtttc tgatacgctt gatattgtct
      121 ttatgccgat aaaagataaa ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg
      181 ggcttagcca aaatcacagg agctacataa ggaaggacga tcgtagataa agtgatcaat
      241 actgcactga tcatactgct caaactcacc atacttgtac aaaataaact aacgataaag
      301 atgatacaag aataaacaaa aaatacagga ttaaatccta aaagcattcc tgcacttgtc
      361 gcaacagctt ttcctccttt gaacccagca aaaatcggaa atgtatggcc aagaatggcg
      421 cataccccaa accaaagagg gttaatccca gaaaggtgga agatcatcgg caaacaagta
      481 gccaatgtac cttttaaaat atccataagc aggacaacaa tcccggctgt tttgcctaat
      541 acacgaaatg tattggtcgt tccagtatta ccgctcccgt gttgtcggat atcttttttg
      601 aaaaacaatt tgccaatcca gacaccggaa ggaatagaac ctaaaagata tgctaaaata
      661 agtaataaga tcaatttcat tttttaccct ctttcaagtt gaaaactatc ctttatttta
      721 gcatgaatag gtaacaggaa caattgaaaa aatgaaagat acaagtataa tagatggaat
      781 aaaaagaaaa gcaaggggtg gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt
      841 tcaactattt ggaagaagca aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac
      901 gaacaagtta catggttgca caggtattgc aagacaatgg atataaaatc ataccagtaa
      961 atccattatt ggctggtgaa gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa
     1021 tcgatgatcc aatcgatatc gtagatgtat ttagacggag tgaattttta cctgaaatag
     1081 caggtgattt tttgaaaact gatgctaaag tgttctgggc acaattaggg attgaaaaca
     1141 aagaagcagc tgagatgcta agaaaagcag gacgtacaga tatcgtaatg gatcgttgta
     1201 ttaagattga acttggtaag ttgaaagaaa accaaaaata aaaatgacca caaagaagag
     1261 atgatcgata gaggggaatg tcgaaaaagc ggttaggaga taattaaaat tgatttctcg
     1321 tctaccctct tttttttcac gaccttttct agtatgataa tcgtggtgcg gttttgccgc
     1381 aaagtattat tgaaggagcg tttgccttgg caaaaaaagt aaacaacgaa tacaatgact
     1441 cgtccatcca agtattggaa ggattagagg cagttagaaa aagaccaggg atgtacattg
     1501 gatccactga cagccgaggc ttgcatcatt tagtctatga aatcgtcgat aatgcagttg
     1561 acgaagcatt atcaggttat ggcagtgaga ttgatgtgac catccatgaa gacaatagta
     1621 taactgttgc agatagcgga cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa
     1681 cagttgaagt gatttttact gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca
     1741 aaacttctgg tggactgcac ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc
     1801 taattgtaac gatcgttaga gatggcgtag agtatcagca gaaatttaaa aacggcggta
     1861 aacctgacgg tactttgaaa aagataggaa aaacaaaaaa agcaaacggg acgactgtcc
     1921 attttttacc agatgataca atattcagta caacgaaatt ttcatatgaa attttagccg
     1981 aacggttaag agaatctgcc tttcttctaa aaggcgttaa aatctctctt tctgatcttc
     2041 gtggagaaga accagtcaag gaaatttttc attacgaaga aggaatcaaa gaatttgttg
     2101 attatctgaa tgaagaaaaa gatacattga caccagttgt ttatttttca ggagaaaaag
     2161 aaggtatcga agtagaggtc gcttatcaat acaatgacgg ttattcagaa aatgttttat
     2221 cttttgtcaa taacgttcgg acaaaagatg ggggaaccca tgaagctggt atgaaagcgg
     2281 cgatgacgaa atcttataat gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca
     2341 agaatctgga aggtagtgat ttcagagaag ggttagctgc tgtactttct atccgtgtac
     2401 ctgaaaatct tctgcaattt gaagggcaaa caaaagaaaa acttggtacg ccagttgctc
     2461 gtaccgtagt agataacgtc atcagtgaac aaatgggctt ttatttgcag gaaaatagcg
     2521 aaatgagcca gatgttggtc cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac
     2581 gtaaagccag agaagaaagc cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt
     2641 caggaaaatt gacacccgca caatcccgaa accctaagaa aaatgaattg tatttagtcg
     2701 aaggggattc tgctggcgga tctgctaaac aaggaagaga cagaaagttc caagcaattc
     2761 tacctttacg aggaaaagtc atcaataccg aaaaagctaa aatgcaagat atcttgaaaa
     2821 atgaagaaat caatacgatg atctatacga ttggtgcagg agtcggtcct gagttttcta
     2881 tcgaagactg taattacgac aaagtaatca tcatgaccga tgcggataca gacggcgccc
     2941 atattcaagt attactgctt actttctttt atcgttatat gaaaccgctg attgaagctg
     3001 ggaaagtata tatcgcgtta ccgcctttat ataaagtatc caaaggacaa ggaaagaagc
     3061 aagtcattga atatgcgtgg acagatgacg aattggcagc aatgattaaa aaagtcggca
     3121 aaggatatat gctgcagcga tataaaggac ttggagagat gaatgcggaa caattgtggg
     3181 aaacaacgat ggatccgact tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc
     3241 aagctgaacg tcgagtaacc acactgatgg gtgacaaagt agaacctcga agaaaatgga
     3301 tcgaaaatca cgtgcaattt acgctcgaag aagatggcag tatcttagat aaaaaagagg
     3361 atactgaaat ctctccttca gtttctaatg atttattaga tgaagaacga gcagataaaa
     3421 atgaaaacaa tcagttattt gaagtagaat aggaggtcac ctgatggaaa atcgacaaga
     3481 agtacaagaa ttaactttag aagaagtgat gggtgaccgc ttcggacgtt attccaaata
     3541 tattatccaa gaacgtgcac tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg
     3601 gatattattt gccatgaaca aagatggcaa tacgtatgac aaaggattcc gtaaatcagc
     3661 aaaatctgtc ggaaatatca tggggaatta tcatcctcat ggtgaccgca gtatttatga
     3721 agcgatggtc cgcatgagcc aagactggaa gttgcgggaa gtactgatcg aaatgcacgg
     3781 gaataacggg agtatggacg gtgatccacc tgctgcgatg cgttatactg aagccagatt
     3841 atccaagtta agtggagaga tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg
     3901 gaattttgat gatacagaaa aagaaccaac agtccttcca gctcgatacc caaaccttct
     3961 cgtcaatgga tcgacaggta tatctgctgg atatgcaaca gaaatcccga ctcataactt
     4021 agctgaagtg atcgatggca caatctatat gatcgatcat ccacaagcga gccttgaaaa
     4081 attgatggaa tatattcctg gacctgactt tccaacagga gggatcttgc aaggaaaaga
     4141 tgaaatcaaa aaagcttatg aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa
     4201 gatagaggca atgaagggta ataaacagca aatcgtgatc acagaaatcc catatgaagt
     4261 aaataaagcg accttggtga aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg
     4321 aatagctgaa gtacgagatg aaagtgatag aacaggtctg caaattgtcg tcgaattgaa
     4381 aaaagacgcc aatgctcaag gaattttgaa ttatttgttc aaaaacactg agctacagat
     4441 caattacaat ttcaatatgg tcgctatcga ccatatgact ccgcatcaag ttggactgaa
     4501 agacatttta agaagttata tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt
     4561 cgatttagcc aaagctcaaa aaagacagca tatcgtagaa ggattgatga aagctttatc
     4621 gattctggat gaagtaatcg caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa
     4681 gaatttagtc gacgtctttc aatttacaga agaacaagca gaagcaattg tcacattgca
     4741 attataccgt ttgacgaaca cggatatcac agagcttcaa aaggaatccg aaagcttgat
     4801 tgcgcaaatc acagagttga ataaaatcct ttcaaatgac aaagagcttt tctctgttat
     4861 gaaaaaagaa ttgcgagaag tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga
     4921 agacgaaatc gaagaaatca aaattgatac gcaagtctta gtggcacaag aagacgtaat
     4981 tgtcagtgtg acgcgagaag gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc
     5041 caaaccagaa gaaatcggga tgcgagaagg cgactacttg ctatactcag gtgaattgag
     5101 tacattggac catgttcttc tgatcacaaa taaagcaaat gtgatctacc gccctgtcca
     5161 tgaactaccc gatttgaaat ggaaagatgc tggtgagcac atttctcaaa caatctccaa
     5221 tctgtctgta gacgaatcga ttttagctgt tttcccatat caaaaaattc aagcagagaa
     5281 aacatttgta ttcatcagca agaatggatt gatcaagcaa acacgaatga cagagttcga
     5341 accatggcgc acctataaga gccgtccgct cagctgtatg aagctaaagg cggaagaaga
     5401 tgaactgatt gctgtgtatc tgaaagaaga tcagccaggc ttagatgttt tcttagtaac
     5461 gcatcaagga atgggtttgc gctatccttt aacagatgtt cccgttgttg gagcaaaggc
     5521 agctggagtg aagtctatga atttgaaaga tggagatagg attatcaacg ggctgcttgt
     5581 gttagcagaa ggtgatacgc cgatcgtaat cgttactcag cgaggagctg ttaaacgcat
     5641 gcttgctcaa gaaatcagcc aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga
     5701 gctgaaaaaa aatccgcacc gtattatcta tatgtcggaa ggaagatcaa aagaaataac
     5761 cttgatgaat caaaaagggc agcagttaac gattgaccca actgatttcc ctataggtga
     5821 tcgaacatcg aatggatcat ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt
     5881 tatcgatgct cctgagat
//
LOCUS       DACBZN010000133         5799 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.134, whole genome shotgun sequence.
ACCESSION   DACBZN010000133 DACBZN010000000
VERSION     DACBZN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.134"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            61..813
                     /locus_tag="IVD64_002580"
     CDS             61..813
                     /locus_tag="IVD64_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6149790.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVSKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            829..1584
                     /locus_tag="IVD64_002581"
     CDS             829..1584
                     /locus_tag="IVD64_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149791.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            1597..1857
                     /locus_tag="IVD64_002582"
     CDS             1597..1857
                     /locus_tag="IVD64_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149792.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            1854..3944
                     /locus_tag="IVD64_002583"
     CDS             1854..3944
                     /locus_tag="IVD64_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6149793.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            3971..4435
                     /locus_tag="IVD64_002584"
     CDS             3971..4435
                     /locus_tag="IVD64_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149794.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            4432..4674
                     /locus_tag="IVD64_002585"
     CDS             4432..4674
                     /locus_tag="IVD64_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149795.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            4692..4994
                     /locus_tag="IVD64_002586"
     CDS             4692..4994
                     /locus_tag="IVD64_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149796.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            5064..>5799
                     /locus_tag="IVD64_002587"
     CDS             5064..>5799
                     /locus_tag="IVD64_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149797.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNE"
BASE COUNT         2114 a          912 c         1188 g         1585 t
ORIGIN      
        1 ggggcaggcg caatttctgc agttgtttac tttaagaaga aagcctagaa ggatcagcta
       61 atgattatca gacaccctaa gaaaaagcga atcatgggta aatggatcat tgctttctgg
      121 ttactttcag ctgttggtgt cttacttttg atgcctgctg aagcgagcgt ggcgaaatac
      181 caacaaaatc agcagattgc cgcaatcgat cgaacaggaa cggcagcaga aacggattct
      241 tctttggatg tttctaagat tgagttaggc gatcctgttg gtattcttac aattccaagt
      301 atttctttaa aactaccaat ttatgacgga acttccgaca aaattttaga gaatggtgta
      361 gggattacgg aaggaactgg cgatattaca ggaggaaacg gtaaaaatcc tttaatcgca
      421 gggcacagtg gactttataa agataatctt ttcgatgatt tgccttcagt taaaaaggga
      481 gaaaagtttt atatcaaagt tgatggtgag cagcacgctt atcaaatcga tcggattgag
      541 gaagtgcaaa aagacgaact acaaagaaac tttgtcactt acttagaacc aaatcccaat
      601 gaagatcgag taacgctaat gacatgtacg cctaagggga tcaacacaca ccgtttccta
      661 gtgtatggga agcgagtaac ctttacaaaa tcagaattaa aagatgaaga aaataagaaa
      721 caaaaattat cttggaaatg gcttttagga agtactgttt ttctttcagt catgatcatt
      781 ggttcgctat ttgtttataa aaagaaaaaa taaaggaggg taaccaacat gagaaataga
      841 aaatggctat ttgtttgtgc agccgctctt ttttctttca ctgcctacac acagttgacg
      901 tttgcagaca gtcaagaaag cacggatagc acagaacaaa caatccaaac gtcagatagc
      961 agcacgattc aaagcagcga tgaaaccgtc gattcgtcaa cagtggattc ttcagaagaa
     1021 ccgaaaattg ctgaagacga tcaaatagtc aaacagacca ttcacgttca aaagattatt
     1081 tcttcagaac aattagacaa ccaggggcat gaaaaacaag aaggagtaaa tggcgcgaag
     1141 ttttctgttt atgatgtatc ggacatttta caaaagatgg atgttaagga tctcactact
     1201 gaccaaattg aatcccaatt aaaagatcga gtgaaaaaac tatccagtga tcaattgaag
     1261 ctagtttcta atggagaaac aaagacaatt gatcaacaaa caggtgtctt tgaattctcg
     1321 gtagaagtac aggcaaatca gaaacaagct tattatattg tcaatgaatc aagtcctgaa
     1381 aacatttcta atagcgagga tattttgcta ttaacacctg tttctgaaaa aaacggtgaa
     1441 ttcttaaagg atgtgtggat ttatcctaaa tcagaagctt cgcaacctaa agaagaagta
     1501 aaaaaaatcg tttctactgg tgtaaagaaa aacttctttg agaattgttg ggattttgtt
     1561 actcacttgt tttctagaga ttaaggaaga aataagatga aactgatcaa attattatta
     1621 ttcaatcttt cttgtttaac cgcagtcttt ttgtactgcg gtttattatt ttctcaatta
     1681 tctattttgc caagctacca actataccta atgaggattt taaacatacc agctgcttcc
     1741 atgccgctag atattatgga aaagctcatt aggaatccat tatttactac gatcaatttg
     1801 atcgttattg tcggaatttc actagtcatt tataaaaaag aggggatgaa gggatgaatt
     1861 ttaaacactc gtggaccaga cggttaaaat atgtttttcc cataatgatg atgttaggta
     1921 cattgtttgg gttaaaaaca gccaatgtct ttgctgaaaa agttattgtc gatccgtcaa
     1981 atccaatcca gaacgtaaag aataatttta ttattccaaa agatgttcct aatgcaaaaa
     2041 caaatattac tgtaaacgga gcgtccaaag tttacaaata tcatgttgat gccaacaaag
     2101 gtggttttac agacgactac gtaggattag tggagggaaa caaaaatata cgctatcctt
     2161 ctttccaaaa ggaatacggt gaaacaaacc ttgttttaga aggcgtaaca accaacacca
     2221 aagtcaatat tgactatgga aaaatcggta cgtataacgg gaaaaaagtc aatatcaagc
     2281 tagttctatc taatattcac ttatactcgg atacgttgcc gtggaatcta ctagataaca
     2341 actatacaaa aactcatttt cgagacgatg gctataaaaa tacgaatggt gcaatgagta
     2401 aatctaaaaa aagaacagta ctttggatct cagacaatct tttctcagga attgtttacc
     2461 attcaacaca aatgaatgtg cagctagtag ccacatatga ggacggtagc cccgttcaat
     2521 tttcaggaga tacatttatc agttttaact ctttgaaccc tgcaggagga aaaagcacgg
     2581 atcttaaagg agaatatgcc cactatgaca aaatgaatac gacagattgg tatgtcagac
     2641 aagatgcggt tctttcagaa tttaaatctt tctacaacaa tctgaacgtg gtaggtggcc
     2701 acccaggagg aagctctaag ctgacacaag cggataatga tttcaataat ttgcatgata
     2761 aattaggaga tccgaagttt ggtcaaggta ctgtatcctt taaaatatct gaagcaaatc
     2821 cgacgtttgt gattggttct tctaatgttc aaacctggtt tactttatct tctgcgacaa
     2881 tctttagtgt tgttcctgat caacctgaaa aaacaggggt tgataaaaat ggaaataacg
     2941 tgaacgataa aatgttgcaa gtaggagaca cgattcaata tcgaatcaaa cagaaggtga
     3001 atcgattagg agtggattta ctagcggttt acgatcgatt tgagttaatc gataatttac
     3061 caaaagaagt agattacgtc gatgcacacg ttgaaagtgg tacaaataaa aaattcgatg
     3121 tatctggtga agtgacctat gacaaaacaa aacaccaggt aaaatatgca gcgaaagctg
     3181 atacattaaa aaatcgaatg aagtataacg gcgaaactta cgaattagtg atcaatgtaa
     3241 aagtcaatga attggccaat caaaatagtg tagccaaaaa tcagggaacg tcgatcatta
     3301 ataaagtaga aaaggataca aatatcatta ccgtttattt ccctaaaatt ccagtgaaag
     3361 aagtacaaca aaatggaaaa gatgtaaacg gacgaaatga cggtaagaag ggtacgccga
     3421 cagctccttt aaacgcagga tctgaagtac aatatctagt cacacaaaaa tggcacacaa
     3481 aaggcgtaga cgctgcgtca gatcactaca aacaatttag tattcaagat ccaattgaag
     3541 ctcgtttgac atataaagaa ggttctgcgc aagtaataga taagagtaaa ggaaaagata
     3601 ttacttctga aggcacacta acctatgata gcaattcaag aacgctgaag tgggaagctt
     3661 ccgctgactt cttaagtaac aatcttttag atggacgaga aattcagctg atatttacag
     3721 ctaaaactcc attacaatca gaaaaaaata ttgataacca agccgtagta gcagtagata
     3781 atgtttctaa taaaacaaat gttgtgacaa ttggggttga tcctaacttg ccacaggtca
     3841 ttgttcctaa aacaggttct acacatttag taacgatttc agcggtcagc ttactattac
     3901 ttgtgttagc aacttttagt tatgcggttc tgagatacat ttaaaataaa aaaacgaaag
     3961 aatgaataaa atgaaaaagt taataatagt agttacttcc ttgattgcca tgattgttat
     4021 tttagtcgga tgtagtaatg aaaaaaataa acaaactaat caagataatg gcgtattgaa
     4081 atcaggaact atgtggaaag aagaagtagg tggtcttgtt tataatttga aaattataga
     4141 cgagactacc tgggaatact ctgagagtgt atggcatcca gatcctgtac aaataacagt
     4201 caagaggcaa aaagactata aaggtttaga aagatacaaa atagtagatt cagctggtgt
     4261 tagggaattt ataaacaaat ctgattcttt atttattgtt gttccctatg agaagaatgg
     4321 cgtaaaaaaa ataatttttc ttggatctag caaggatgaa aagcgaacga aagagaaatt
     4381 gattcacgat ggatcacaat ccaataaata taagttacaa aagactagcg agtgattgct
     4441 agtcttttta tttgtcctga aggaggaatc acagtggcaa aagctaaaac gccatttgaa
     4501 aaagccgcgt ctaaaatctt agaacgccgc ggtgtatctg aaacacagtg gaaacaagaa
     4561 gtccttgaaa aagcacaatt tgacctatta cggggaaaag acaaggactt agaaggatat
     4621 atctatcaac gtgaacgtga aaagcttgtt ttcgatgaaa ttattaaaca ctaaaaggag
     4681 cgaacaattt tatgtttgaa tcaattacaa tgaatttttt aacacaaccg aaagtagtag
     4741 ctgcggttga tatttttaga gcattacaaa ctgccgcaac tagtttaaaa aacaatctga
     4801 tgatttttgc tgctgctgcg gccgttgctt ccattgttat tgggggtctt ttcttcctat
     4861 ttggacgaag aggagctgaa aatgggaaga tgcacgtagg ctatgtattg atgggggtag
     4921 gagttatttt agcagcagct tctattgtgg tttcgttatt tggattgttt ggacaaacac
     4981 ctcaaggatt ataagaaagt aaaaaagtag gagtaagaag tagtcgatac tttttactcc
     5041 ttttttatga aaggagaata ctcatgtact ttacgattgc aggcaatacc aaaacagata
     5101 aagagttaat aaaaaaagca acgaaggaag taggaaagcg tacttctttt ttaccgaaaa
     5161 caattaaata ttctgtcatt gaaacggaag aacagctaca tgtgttaggg aaagtactgg
     5221 aacacgaaag tcgatcttct gttaagttga ctctcacgtt acatgacaca gaaggaaaaa
     5281 tagagaaagc tggtacagaa tacgtttttg gcccactgga ttatcaaatt tcagaaggtt
     5341 tctatcctac attgatggat ttaatccgcc ttgatgtgtt taaagaaacg gatgaaacta
     5401 tgagtgtgga agagtcttat cgaaacgaaa aacttttaca agttttagaa gaagagttag
     5461 agagcgacca aagaaaagtg aatcaaggga gtgaagtcga agtaaatgaa gaagtaccat
     5521 gggaacgacc aatatttcca aagaaggagt caaaagaaaa agagacagtc tatgttgacg
     5581 aacaagctga tattccgact cagaaatcga tatttgagga agagaacgat gtgatgggtc
     5641 aattggaggt agatcccttt gaagaaacaa tggaggatga agagacggcc gaagtagaag
     5701 ttgatccagt tgaagcgaat gaagagtcaa catggactga aggggattct caggaatata
     5761 ttgaaccact tatcagtgaa gaagtagaaa aaaacgaaa
//
LOCUS       DACBZN010000134         5666 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.135, whole genome shotgun sequence.
ACCESSION   DACBZN010000134 DACBZN010000000
VERSION     DACBZN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.135"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(153..1193)
                     /locus_tag="IVD64_002588"
     CDS             complement(153..1193)
                     /locus_tag="IVD64_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6149798.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1542..1868)
                     /locus_tag="IVD64_002589"
     CDS             complement(1542..1868)
                     /locus_tag="IVD64_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149799.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1855..2643)
                     /locus_tag="IVD64_002590"
     CDS             complement(1855..2643)
                     /locus_tag="IVD64_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6149800.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3011..3301)
                     /locus_tag="IVD64_002591"
     CDS             complement(3011..3301)
                     /locus_tag="IVD64_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP6149801.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3437..3616)
                     /locus_tag="IVD64_002592"
     CDS             complement(3437..3616)
                     /locus_tag="IVD64_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149802.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(3613..3963)
                     /locus_tag="IVD64_002593"
     CDS             complement(3613..3963)
                     /locus_tag="IVD64_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149803.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3956..5281)
                     /locus_tag="IVD64_002594"
     CDS             complement(3956..5281)
                     /locus_tag="IVD64_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6149804.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
BASE COUNT         1681 a         1021 c          812 g         2152 t
ORIGIN      
        1 ttaaaaccta cgtttgtgat tgttttcata aacttttaat ctaaaaactc cggaataata
       61 gctatttatt tgtaacattt gtaataaaat taaaatcata aaaaagttct aatttaaaat
      121 tgatcatttt tatagtgatc gctttttatc aactactcta gccaattttc cgtactaact
      181 gttggtaatt cgttaaatgt ttctctttgt tcgtttgcaa gctttgccgc aatagctttt
      241 tcgacaaacc aattttttac ataaacgaaa attagattct gcaaattgtt tactttttca
      301 gttttttgtt tttgataagc tttcagcaaa gtcatatata atcctttgtc agcatcaatt
      361 ccataagatt ccagttcttc ataaacaaca actgtttgct ctaattcttc agcttttttc
      421 tttgcattat gaatagtttg gacggttttt tgagcgtctt cgtaagtact agaaaatgtt
      481 tttatcaagt ttaatacatt ttctggtaca aatgtattta tactgtcctc tttcatgatg
      541 tcgacgaagt tatctagtaa aacttgattt tgtaactgat cagtttcagt gtctattatg
      601 tgtctattag tgtctataga attagaatag aatagattgt ctgcgatttt cgcaattacg
      661 gttgagtcct tgatatgact ggattttgcc gtatttgcga ttttcgcaat tacggtggta
      721 tcattgattt ctcgcggttt tgccgtattt gcgattttcg caattacggt ggtatcagta
      781 ttgtcttcct cttccatgat tttaaagacg tcttgattcg ttacttctgg cttgcctaag
      841 tacaataaat taggcatgga ttcaatctta ccgtcacgtt tatttactct tcctctcttc
      901 tgtttcagaa gacctgcttt ttctaattcc tttttgattt tagatacttt accttcgcgg
      961 cattgtaaca atttcattaa ttcttcaact gtgaatatga aaaatatatt atcatcagaa
     1021 tctacccagt tatttttgac tgaataatca aatcgatctt taagtagcat ataagcaatt
     1081 tttgcatcat ttgatagatc catgtatttt tcgttagtaa aaaatacctt aggcatttga
     1141 taatattttt cacgataaat ttcttttgat ttgtaataat taaaatcatt catagccaat
     1201 cctctttctg taggatagcg ttttgttcaa aactatgtta caatgaatta aacaaatata
     1261 tctatccact atatattttt ctactgctga taagtcgcca aactttgtta aagcagtagt
     1321 ctttttttat ctttttttag tttaccttaa aaaataaatt aaagcaaacc gctctatttc
     1381 aaggtttaag ctcatagtaa ctatatcacg atattacaat aatattatat cacgatatcg
     1441 taatattaca atatgaagat aagcgactta tcagctttga tactactgat ttttataaaa
     1501 aaaatatcac gatattatga tatcgtgata ttgaatgtaa attaaatttt attttgtttc
     1561 tttgctagtt tatatccttc aatcaagtat tcttcaggta tggactttaa tagcgcatct
     1621 tctataacgt ctggtttact tttgtctgat tcagcaatag tcgtccaata tttgagtgct
     1681 gacattagaa ttggatttat acgaatgcta tctgctgttc ttacactact tccttgatct
     1741 tttatgactt tacttatatt tttcttggtt aattcgattt cttctacttc ttttgattta
     1801 aaattctttt tatttttatt tcctagtaat ctacttactg cagcttgact gcgattatct
     1861 tctgccatca aaatcaccaa cttcttttaa atattcttct gtaaatgctt tatagtcatc
     1921 ttcagctgtt gagggtttat taaaattgat tagatctgat ccagcagctt gcgcttcgtc
     1981 aacggttatt gtcctacgaa tataagtttt gaaaattttt gcaccaatga ctctagaaat
     2041 ttcggttgct gcagacgtca tttctttaga aatgttataa cgcttattat acttagtaaa
     2101 tagaactcct cgtacttcaa gcttgtcgtt cgtatattct tttaccgttt caatagtttc
     2161 acttaacttt attagtcctt ttaatgagaa atacgcagct tcaactggaa tgataatttc
     2221 atcagaaaca gtaaatgcgt tgactgtaag cattcctaaa cttggtggac aatcaattat
     2281 aatataatca tagttgtcct tgattggttg aagtactctt ttaagcctgt gttcacttcc
     2341 tacttgagta aattccctct ctgcagaaga aagggctatg tcggctggca aaacatcaat
     2401 tcctttatag tccagaatag catctgtaat gtcagcctcc ccttttaata cttcataaat
     2461 tgtaggttgg tttgttccat ctatatcgaa acctacatta tctgtagcat ttccctgagg
     2521 atcaatgtcc actaaaagta catttttacc gtaaacaaat ttcaaacatg ctgctaaatt
     2581 aacgctagtc gtagttttag aaactccacc tttttgattg gctactgaca atattcttgc
     2641 cattaaaata tcccccattc atcttatatt ataatataat tatatcataa tattgtgata
     2701 ttgcaatatc gtatatgcat tatattgtaa tattgtgtga ttattttgtt ttaaagatat
     2761 atatcggttc ggatataaag attattcata tgttattcga aattaaaata atcacttttt
     2821 attactttaa aattttttat taatgataag aaatatgttt tgcgagcagc aactagaata
     2881 atttaaacat tactaaactg tctatattgt tactaataat tcccttctta tagtgcattt
     2941 tcttagaaaa aaacaatatt acgatatcat gatattaaga tatcgtaata ttatacaatt
     3001 gtgattgata ttatatatgt ttttttattt ttttatcatg tttaatcttg aaggaatcat
     3061 attctttttc taaagacagg agcttttcat aaacatcaga catgtttaaa ctagaggcca
     3121 ggcttaaatc ataaacgaat gaagcaggta atgattttat tctacgttgt cgggttatcc
     3181 agcttgcaac cgttccttgt gggtaggcat ataactcaca aaattgttct acagtgattc
     3241 ctaaattctt gataatgtaa acattgatcg ggtgcgagta aacaaatgta ttgatagaca
     3301 tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa taatagttta acacacgcat
     3361 tattctagta tctactccta tttgtaaaaa caaaaaagga ttcatagaga taactctata
     3421 gaatccgtaa aataccttat atatataacg ggaagatata aaacctaaaa ttatgataac
     3481 ctgctgcagc ttctacatag aaagagaagc gctcaacacc aacctcattt atcaaacgtt
     3541 ggccataacc ccaaatctct tctgatagct gatcaaaggt tatatacttt agctcatatt
     3601 gctggataat gttcattcta atcgactcca tttattatcc tggtaaaatt cgatatgacg
     3661 tatttcgtcg tagtgaattt tgtcttttcc aacgtagatc cccagctcat cactacctaa
     3721 gatcttgcca atgatatctt ctggatagta tccttcagcg tttcgttctt ctttttgaat
     3781 agatactgat ctatttttta tgactgcagc gttcaatact tccgcaatct cttcagtcgt
     3841 catttgttct tttgctaaat tttgactggt tcgttgttca ttgtcggcat caatttcttc
     3901 agtatgatca gataaataaa accctgccca ctttaaacgt ccacgatcat tgtaatcaat
     3961 catttttaat tcccccattt ccgcccgcat gaccaccaac aagtgtgctt ctagtaatgc
     4021 ttctagcgcc ttctaacatt gaagaagcat gtacaatcga cgtaaaacca tatttttgcc
     4081 gaatcttgtc aataatttta tctaaatttt cttcgtcaat ttgcttttgt ggatcactaa
     4141 ataaatccag ctgaagcgaa tcggtgtaca caagcttgct ataggttatt ccaacatgcc
     4201 ttacttcttg tccttcataa tacttatcaa acaagaaaag acaataattc tgcaattcct
     4261 ttgtattatc tgtgataggg atcttcatct ggtgtgaaaa ccctggtctc gtttctaaga
     4321 tcgacgtgcc aatgtgtaaa tggacacaac ctgttttaca gttatgctgc ctaatgcgta
     4381 tcgctacttg ttccgccatt tcttttacca ccagctcgat ttcatttctt ctagtataat
     4441 ctcgtggtaa cacttgtgaa tttccatatg atttttcctt tgtcggttct ggtgggattg
     4501 ctatatctgt tcgatcgatc ccattagcat ggaagtacag ctgtaagcca atcacaccta
     4561 gtctattctt aatcgtataa ggattccagt tagcaagatc tctaatgctc ataatcccca
     4621 tctgattgag tctttttttc attcgagagc caattcccca aaagtccgtc atttcaggaa
     4681 tattccatac tttttcagga acgttttcgt acgtccattc cgcaacaaat ccttcattat
     4741 gctttgcttc gttgtctaag gctagtttag ccaaaagggg attatctccg acacctactg
     4801 tagcaatcaa tcctagctct tctttaatat tctcctggat catttgagcc agcttctttc
     4861 tccgttgact tcgtgttcct tcagtcgtaa aaagattcag tgatcgggtc acttttaaga
     4921 ttgattcatc gatcgagtaa atcagtagat cttcatcagc cacatatctt ctgaaaatat
     4981 tatttacctg catatttcgc ttgatatata ggttcatacg tggtggaaca acgtgtagtg
     5041 ttttaggaaa tggttgtggt aagtcacgtg gtctactcac atttgtaata ccataccgct
     5101 tttttgcttc aggagaagaa gctaatatca atcctgaacc agtattatca gctcgactca
     5161 taacaacaag ttctgttgtt aatggatcta aatttctttc tatacattcg acacttgcgt
     5221 aaaagctttt catatcgatt aaaaagtaat catttactgg ctcttttgaa taatccatca
     5281 taaataacac tccattcttt cacattatac aaacatatgt tcgatattgt aaagaagaaa
     5341 tagctaaata cataaaaaaa gaagcatagg tatgctatca ccctgcttct tcttttgatt
     5401 tgtatgatca atttattcta tcactttact aattaatttg aaaatctaat aaaaactctc
     5461 actctaaaag agtgagagac tgcaaattag agtacggact tttttggagt tccgttcttt
     5521 aatattaaca aatgaataaa atccttacaa catttgagaa taagttatca gtagacactt
     5581 acaaaaaatt ctcatactat ttcctccatc attagttttt gtctatataa ggtttaccct
     5641 aaatagacac tggtataaca ttgaaa
//
LOCUS       DACBZN010000135         5585 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.136, whole genome shotgun sequence.
ACCESSION   DACBZN010000135 DACBZN010000000
VERSION     DACBZN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.136"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(488..1297)
                     /locus_tag="IVD64_002595"
     CDS             complement(488..1297)
                     /locus_tag="IVD64_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6149805.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="IVD64_002596"
     CDS             complement(1297..2043)
                     /locus_tag="IVD64_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6149806.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="IVD64_002597"
     CDS             complement(2061..2528)
                     /locus_tag="IVD64_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6149807.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="IVD64_002598"
     CDS             complement(2528..2938)
                     /locus_tag="IVD64_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6149808.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="IVD64_002599"
     CDS             complement(2949..3962)
                     /locus_tag="IVD64_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6149809.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="IVD64_002600"
     CDS             4179..4913
                     /locus_tag="IVD64_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6149810.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..>5585
                     /locus_tag="IVD64_002601"
     CDS             4897..>5585
                     /locus_tag="IVD64_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6149811.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1790 a         1184 c          920 g         1691 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatc
//
LOCUS       DACBZN010000136         5502 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.138, whole genome shotgun sequence.
ACCESSION   DACBZN010000136 DACBZN010000000
VERSION     DACBZN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.138"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            279..1127
                     /locus_tag="IVD64_002602"
     CDS             279..1127
                     /locus_tag="IVD64_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149812.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="IVD64_002603"
     CDS             complement(1298..1639)
                     /locus_tag="IVD64_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP6149813.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="IVD64_002604"
     CDS             1725..2432
                     /locus_tag="IVD64_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP6149814.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="IVD64_002605"
     CDS             2419..2742
                     /locus_tag="IVD64_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6149815.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3865)
                     /locus_tag="IVD64_002606"
     CDS             complement(2831..3865)
                     /locus_tag="IVD64_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6149816.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
     gene            complement(4312..5295)
                     /locus_tag="IVD64_002607"
     CDS             complement(4312..5295)
                     /locus_tag="IVD64_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP6149817.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT         1609 a         1142 c          981 g         1770 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgtacc tgcgtaaaaa acgcgatcgc
     3601 cgacagaaaa tttttgcact tcgtcaccta cagcaacaac tttcccgact ccatcaaaac
     3661 ctaagatacg taaagcattt cttatcccgt tatcttggcg aagtttcgta tcgacaggat
     3721 taacagatac tgcgttgact tttaccagaa gatcccgtga actgggtaca ggtatctgtt
     3781 ctttttcatc aataaagctt tttggatcat cgatcggcaa cccttcataa aaaccgacta
     3841 ccttcattaa ttccttttct gacatacgaa ttcctcctta gcttcatcaa gttggagcat
     3901 ccatacagtg ttcatttcaa tatatatagt atgctatttg ttccaccttt tttattcatc
     3961 ataattattc tcttacttaa agtaaactat ttgcactttt ttggttaaaa aagaggttgc
     4021 gacaagactt ttgtcacaac ctcttataaa cggtgttctc ctaccaactc ttcggtcaca
     4081 agtcaaaata ttcatcaaac atgagaagct gttttttgaa attgctcctt gtgactcaga
     4141 gttaaacggc aggctcacaa cctctgatat aaacggtgtt caatcaactg accctcggta
     4201 ttagctcaaa aaacgacaaa atatgaagag ctatttttgt ttggctgttc ttattacttg
     4261 atacaggaca gctttttcac gacctctaat tcacggtgtt caagcaagaa atcattgttc
     4321 actgaaaagc gttggatgtt ctttttcaaa ggtctctaag atctctcgta cttcatcagt
     4381 agatttcgta ttcatcaact gaactcgcaa gtcatttgct cctggaaatc ctttgatata
     4441 gattttaaag aaacgatgta gtcctgtgat ggaacgcggg atgatttctg aataatgatc
     4501 ttgcagatct agttgcagac gaagtagtcc gatcagttct ttttctgtat gttccctcgg
     4561 gtctttttca aatgcataag gattcttgaa aatccctcgg ccaatcatga tgccatcaac
     4621 gccatatttc tcggccaatt cttctcctgt ctggcgatcg gggatatctc cattgatcgt
     4681 gattagtgtt tgtggagcga tttggtcacg taaagcaatg attttaggaa tgacttccca
     4741 atgtgcatct actttactca tctctttacg cgtacgcaaa tggacagaaa gattggctat
     4801 atcttgatga agtaggtgac tgatccattc ttccatctca gacatttccg tatagccgat
     4861 acgcgtcttg acgctgacag gcaaaccgcc agctttagct gcttcaatca attgtgccgc
     4921 tacttctgga cgaaggatca agccactgcc ttttccacgt tctgctacat tagggactgg
     4981 gcagcccatg ttgatatcga ttccttggaa gcccatttct gccatagcaa tgctcatttc
     5041 tctaaaaaat tctggcttgt ctccccaaat atgggcaacc atcggctgtt catcttctgt
     5101 aaatgttaat cttcctcgta cactgtcttt tccatctgga tgacagaaac tgtctgagtt
     5161 tgtaaactca gtaaagaaga catccggtgc tgctgcatgc tttacgacat ggcgaaatac
     5221 aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa ggctttggca gctctttcca
     5281 aaaattagta gtcatttatt tgttactctc cattctatca agcagtgaag tcgcaatctt
     5341 tctcaaaaac attgtcatta tactaggaaa caaaaaaaga agcaaagtga acagcttaaa
     5401 agatgtttct gattgagcaa aaaaaagact gtgacaaaaa tcttatcaca gtccattttc
     5461 cggaataaat ggtgttcaaa agcttgcatc tgcggtaata ag
//
LOCUS       DACBZN010000137         5441 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.139, whole genome shotgun sequence.
ACCESSION   DACBZN010000137 DACBZN010000000
VERSION     DACBZN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.139"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..1057
                     /locus_tag="IVD64_002608"
     CDS             <1..1057
                     /locus_tag="IVD64_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP6149818.1"
                     /translation="KYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTP
                     NSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEE
                     TTILTQVQQLQLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQV
                     LEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQK
                     EYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQTTFLAN
                     IQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQKV
                     AACTSEACDFKRSEGFI"
     gene            1403..1999
                     /locus_tag="IVD64_002609"
     CDS             1403..1999
                     /locus_tag="IVD64_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6149819.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            2012..2911
                     /locus_tag="IVD64_002610"
     CDS             2012..2911
                     /locus_tag="IVD64_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6149820.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2914..3045
                     /gene="ssb"
                     /locus_tag="IVD64_002611"
     CDS             2914..3045
                     /gene="ssb"
                     /locus_tag="IVD64_002611"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6149821.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            3067..3399
                     /gene="ssb"
                     /locus_tag="IVD64_002612"
     CDS             3067..3399
                     /gene="ssb"
                     /locus_tag="IVD64_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6149822.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            3444..3677
                     /locus_tag="IVD64_002613"
     CDS             3444..3677
                     /locus_tag="IVD64_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149823.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3836..3958)
                     /locus_tag="IVD64_002614"
     CDS             complement(3836..3958)
                     /locus_tag="IVD64_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP6149824.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            4148..4372
                     /locus_tag="IVD64_002615"
     CDS             4148..4372
                     /locus_tag="IVD64_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149825.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            4815..5021
                     /locus_tag="IVD64_002616"
     CDS             4815..5021
                     /locus_tag="IVD64_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6149826.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            5021..5272
                     /locus_tag="IVD64_002617"
     CDS             5021..5272
                     /locus_tag="IVD64_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149827.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
BASE COUNT         1973 a          945 c         1121 g         1402 t
ORIGIN      
        1 aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta
       61 tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc
      121 tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat
      181 cattcctaca aaaacggtta tgacggcagc ggcttttgaa caattaagtc cactgcagca
      241 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
      301 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
      361 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
      421 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
      481 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
      541 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
      601 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
      661 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
      721 cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc gccaaatggg aaagttactt
      781 attaaagatt ggtgagcaga aaggaacaca aaccactttt ctagccaata tccaaaaatt
      841 tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac
      901 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
      961 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     1021 agcctgtgat tttaaaaggt cagaagggtt catttgaagc aacgattgtt ttaaaagaag
     1081 acttcactac aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag aaaagaacac
     1141 gccgcactgc aaaataatgg agggttaatc ttatgcgtaa taccacacac aaacaacaag
     1201 aaatcgaaaa tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt gccagcaaac
     1261 gaagactgca gcaattgaag aaccagcaga ggattcaaaa agaaatcgaa ttagatcgat
     1321 aaaatcttcc taactattgt atacgtataa acttatgtta tactgttttt ataagtttat
     1381 acgtatacaa aggagggaga aaatgacaaa agcaatggta tacaagctgc tagaagaatt
     1441 taatggaact ttggctttga aggatgccaa aaaagcgggc atttctcctg taacgatcaa
     1501 acgtatggta gatcgagggg agctggatcg agagtatcca ggttttttca ccttacctgg
     1561 tcaatttcct gatgaattgt ttatggcgca aaaaaaatat gaacgcggca ttatttcaca
     1621 tattaccgcg ttagatttgt atgatttaac agatatgatc cctagacaga tcgatctgac
     1681 tgtaccttac ggttatcacg tttctgaaaa aggattgaag gaattcgcag ttgagctaca
     1741 ctatacaaaa gcggaatggt atgaattagg taagattgaa ataaaaagtc gctatgggaa
     1801 tcctattata gcttatgatc cagaaaaaac cttgtgtgac atttggaatc cctggtataa
     1861 cgttgaggat gaaatcaaag taaaagcaat caaaaactac atggagtcgg acagaaaaaa
     1921 tcttagaaaa ttgaatgagt atcgacgaat tttgcctaca gataaaacga tgcgctctta
     1981 tattatggct ttaaattaaa ggagaataca catgacacct acgcaattaa aagcaaaagt
     2041 gaagaatatt tctagagaaa aagaagttga tccgcagctt gttttaagac attttatgat
     2101 ggaaaaattc ttggaaaaaa tctcagagtc tccttatcgt gaaaattttg tgttaaaagg
     2161 cggcttctta attggctcaa agtatggcat tgaaaatcga acaactaaag acattgatac
     2221 gactttacgg gaaatgaaag taaccaaaga aacattgaca acggttctaa acgatatttt
     2281 ttcaacgccg acgaaagaag gcattcaatt tgagattcaa ggaatgaaag aaacgaggga
     2341 agctgattat tatccagggt ttagcttacg tgtactggca catttggaaa acatgcggcc
     2401 agattttaag gtagatgtta ctacaggtga ctcgatctat cctgcgacga tcactcacag
     2461 tcataaattg atgtttgagg atcgaacgat tcctttggaa tcctatccta cagaacaaat
     2521 tattgctgaa aaattaagtg caacctttga ttttttaaca gataattcga gaggaaaaga
     2581 tttttatgat ctctatacta ttcctaaaat ggagaagatc gatgagaaga ccttgaaaga
     2641 tagtgtgaga aatacattta cacgtcgagg aaaaactgat ccgttaaaga tatactatga
     2701 aaaggatatg gatgtactga aaaatagtca cgagctaaaa gatacctgga agaaatatca
     2761 aaaaagcaat ccttatgctg cgtcgatcac gtacgatgag acaatggatt cgattagtaa
     2821 tttgatggaa aaagtgattc acgtcgaaga aaaagagcga aaactagctt atcaaagaca
     2881 ccaacagatg aatagagaaa tggagagata aagatgatta ataatgtgac attagtagga
     2941 cgcttaacca aagatcctga tttacgttat accgcaagtg gtactgcagt agcgactttt
     3001 accctagccg tcaaccgaaa tttcacgaat caaaacggca attgagaagc cgattttatt
     3061 aactgcgtga tttggcgcaa accagcagaa acaatggcga cattagctaa aaaaggtagc
     3121 ttgattggtg tagttgggcg gattcaaacg cggacttacg acaaccagca aggacaacgt
     3181 gtatatgtga ctgaagttgt agcagataac tttcaactat tagaaagtaa agcagcgaca
     3241 gagagccgtg cgcacgctga tcaatcgtcc accagtccaa gcaccgccac ctttgaacaa
     3301 cggaatacag cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga
     3361 caatcaatcg atattagcga tgatgatcta ccgttttagt ttgagaaaga aggagtaagc
     3421 atggcaaaaa aattagtctg acgatggatc tattgatacc cgtaattgca tttatagacg
     3481 ggatttcttt gggcgtcact gaaaatcctt tggtaaatag aattgcgaaa ggatcactat
     3541 ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa aagagcagga aaggataatt
     3601 ccttttggcg attgactaaa gtcacagtag cagcgacagc tgttatactt acggctattc
     3661 taccgcttta tatgtagagt taacgggaaa tcgttgaact tcttgcgaaa tattgctata
     3721 atgaacatga aaaagaggag ttgcgctaac aacccctcat taacagcacc gattaaagaa
     3781 cggtggcaga atattagtta tttggaaata accgctgacc tgctaaagta gacggttatt
     3841 tctttttgtc atttttaagc atatttacaa tcaaactaat caaagcaatg gtaaacatac
     3901 caaaaccaag aatggtttga attgtctcat atgcggacaa aaggcttact cctttcatag
     3961 attttagcac ttacattcat aagcaccacc acctttccag aagtcgccac cgtcttcaaa
     4021 ctttactgtc ttgactatta tagcatatct aaaatcgaat aaaaattctc aattttagct
     4081 gtttttgcta taattttagc gaggtgatcg tgatggaaca atatattggt ttactcgata
     4141 aattagggtg atcaagacac agccattgct tgtgcgattt acattacgca cgagtcatga
     4201 accaatcaat tgtgtagtgg ccaatataga aatagcggat aaattgctga ttttggaaga
     4261 aggcaagtat actatttcag tgacaggtca cttcaataaa cgaaagcaac tagtcattga
     4321 atcaatgcaa attagaaacc ctgatcattt tacgcgatcg atgagcatat aaaagaagag
     4381 ctgcagtcct aaatgaggat tgtagctctt tttgttttac atagaaacag taaaaatgaa
     4441 aaggcctgtt taggaaggca caagcgcctt ctcaattgaa agacctttct ctcactccaa
     4501 ccgccaccgc cgttcgagaa gaaaggaagt gaacgctacg cgttcactaa gataaagggt
     4561 cttgcatagc aagacaaaat agaaaaaatt tcctctcacc aaaaggttcg ctcctgttcg
     4621 ttatctcttt attgatttgt cagaccggca acaagttgcc ttttatcacc aaaaagcgac
     4681 aaggacaagg gtaaatggca cgctgcgcgc ccttgtcttg tcgcttttcg ttgattcatt
     4741 ctggccaaca aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg
     4801 aggcagaatc aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat
     4861 tcccaaacta tcatgcacca catactgtcg aatgcgattg tggagaaaga gcgaaatgta
     4921 tcaaaagcta ttgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg
     4981 gcgattatat tttaattgat gagaaaggaa agaaacgctg atgaaaacac aacacaaatt
     5041 attgactgta aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt
     5101 agcagaagtt ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc
     5161 accgactatg ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga
     5221 aaacttggaa acttatgaaa acgaatattg gttaaccaaa ttagagaact aaaaaggaga
     5281 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
     5341 acaaaaaatg gaggatatga cgttcctata caacgaaaca cgtgttccaa aaaaacatta
     5401 taaagaaaaa ttgtcggtag ctgtggaaga aatgattgaa t
//
LOCUS       DACBZN010000138         5427 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.140, whole genome shotgun sequence.
ACCESSION   DACBZN010000138 DACBZN010000000
VERSION     DACBZN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.140"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(126..772)
                     /locus_tag="IVD64_002618"
                     /pseudo
     CDS             complement(126..772)
                     /locus_tag="IVD64_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(1180..1683)
                     /locus_tag="IVD64_002619"
     CDS             complement(1180..1683)
                     /locus_tag="IVD64_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6149828.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1806..2570)
                     /locus_tag="IVD64_002620"
     CDS             complement(1806..2570)
                     /locus_tag="IVD64_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP6149829.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2678..2953
                     /locus_tag="IVD64_002621"
     CDS             2678..2953
                     /locus_tag="IVD64_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP6149830.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3391..4341
                     /gene="yidC"
                     /locus_tag="IVD64_002622"
     CDS             3391..4341
                     /gene="yidC"
                     /locus_tag="IVD64_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6149831.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4520..4720)
                     /locus_tag="IVD64_002623"
     CDS             complement(4520..4720)
                     /locus_tag="IVD64_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149832.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         1584 a         1135 c          943 g         1765 t
ORIGIN      
        1 aaaggggctg tccccagact ttagtaggcg tcattagcta tctttcgtta gcatttaaag
       61 gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa
      121 taaaattatc gttctttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt
      181 aactgttgat tacattggtc actatcatga caaatataat tccccttttt cgtatacgtg
      241 ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag
      301 attgcacaga ttcccttatt tatcgttggt gataaagtac cgctaatccc ttttaatttt
      361 tcatcttcgt aatagaccat gaattttttc tgcgtacctg gatcattcca gccgatgtac
      421 gtatgttctt gcaagtcgat ttcattccaa gctggttgtt tgagtttttt cacttttcgg
      481 aataatttct cgatttgtgg tttgctaggc tgcataaacg gctgaacgat ttgcttcagc
      541 tcctccaatg ctctttctgc ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa
      601 aatgtatcga ttctggcagt tcgtgatcta atatgtctcg aatcttttca gctgctagtg
      661 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
      721 ctacttctct tattataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
      781 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
      841 gtgttacctg aagatcccgg actcttttaa ataagatacg ttttgcatct tatcatcacc
      901 ccctttaatg cttattattc tatgtacatg attcattcga taaataaaag taaaccgtat
      961 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaaagtc gtgtcaaagt
     1021 cccttgtttc gaatgaatgg tgttcaaaag ctggcatctg cggtaataag ccgaaacttc
     1081 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     1141 tgtcacaacc tcaatatggt ctattattgg ttttatctat taaatcgagg attctttccc
     1201 aaagtaacgt tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac
     1261 tgcgcagtat ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat
     1321 cgttgcgccc cacatgtgct tcaaaatgat atctcgctca agatccgaca attcggtgat
     1381 cttttctgca ttctttacag caattcttgg atggatatac gcatgtgtgc cttcatcaaa
     1441 tttcgtcgta cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc
     1501 tttagcattg ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga
     1561 atgctccaaa cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc
     1621 tgtttctaaa agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt
     1681 cataattatc gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat
     1741 accaccagtg ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc
     1801 cttaatcata aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat
     1861 tccgcatttc ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga
     1921 cttaccccct gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg
     1981 tctaatgcgt gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc
     2041 attaaaggaa tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc
     2101 tgcatgctgc gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca
     2161 ccagccatat caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt
     2221 acattatcta acaacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc
     2281 atcactgcta agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc
     2341 gaaactagaa aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg
     2401 cctcgctgtg tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc
     2461 agatggaacc cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt
     2521 ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc
     2581 tccccatgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     2641 tcattaattt ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt
     2701 ccaaggacgc gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact
     2761 aggcgtgact ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg
     2821 caatgacgac atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg
     2881 acgagtgcag tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga
     2941 tgtggtggga taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga
     3001 aaaatagctc tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga
     3061 acaccgtcga taaggttatt cttgaggcgc tttgcctcaa aaattaagga cagcagtgaa
     3121 aaagctgatc tatcacttgc agtgagttca tttgactccg ctttttcggg cagaaaaaaa
     3181 gtttttagtt tattaaagta agaaagaaac acttggaatc attctacttt ttcctagtat
     3241 ttctttcttg attttttgtc tttttttcaa aatttttcgc tacgtaaaag aacgaatatt
     3301 tatatgaaat ttctatgaag ttgattgaat tccatgcgct tttttagtat agttaagttt
     3361 gtgtaaaaat tatagaagag gaagtattct atgaataaat ggaaaaactg gttattggga
     3421 tccggacttg tcagtatcct gctcttttta tctggctgtg tgcggatgga ttcaaacgga
     3481 aaccctgaca cttcgggaat cgtttatcga gtactcgtcc atcctatggg ccaagcgatt
     3541 acatatctag ttgaaaattt caattggtct tatggctggg cagttattgc aatgactgtg
     3601 atcgtccgta tcatcatctt gccactaggt attagtcaat cgaaaaaaac aatgatccaa
     3661 tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag cccaacaaaa attgaaagct
     3721 gcaacaacac gtgaagaaca aatggcagca caagcagaaa tgcagcaagt ataccgcgaa
     3781 aacggattga gtatgaccgg cggaatcggc tgtctgcctc tattgatcca aatgccgatc
     3841 ttctctgcct tgtactttac ggcacgttat acagaaggta tccgggaatc atctttctac
     3901 ggcatcgatt taggctcacc aagtatggta cttgttgcca ttgcaggggt cgcttatctg
     3961 cttcaaggat acatctcaac gatcggtatt cctgaagaac agaaaaagac gatgcgtacg
     4021 atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga tcagtgcgcc agccggtgtt
     4081 acactttact gggtagtcgg tggtgtgttc agctgtctgc aaacattcat cacaaacgtt
     4141 atgatgaaac cacgtttgaa agcacaagtg gcagaagagt tgaaacaaaa cccaccaaaa
     4201 caagtcgtca ctccaagaat tgatgtgaca gaatcagcta aaccagctga cagcaaacaa
     4261 gcaaccaaac aaatcaagca acccaaaaac agtacaaaca ataaaggccg caacgcagga
     4321 aagcagcaga agcggaagta gatagtaaat aagagtgtaa taaaaattca ttaacagttt
     4381 tagcaattaa aaatatcgac caagattttt gaaaaagaaa tctggtcgat atttttattt
     4441 aagtgaatgt ttaatttgaa tcatcgattg atcgctatct aattgcagca gcatcttttt
     4501 atgaagaaac aactttttct tatctatcaa cgtagacagg tgttgctgtt tcttctaata
     4561 gtgtacttaa ttccttaata ttttgttttc cttcttctaa catccaacta ctcgcttttt
     4621 cttcgccttc ggtaacaaaa ggcgccactc ccttcgccca tgttgctcgc ccacataaga
     4681 caccattctc cataaaggac aacgcggaga taccacccat ctaaataaca tccataaacc
     4741 tttctagtag aagaatagtg atagtatcca tccctttttc tactgggaat acaattacac
     4801 gattcaaagc ttgatacaca acatttccct tcttataaac cgctattgta accgaattgt
     4861 tttgaatcac ctttttggga tagaaactgg ctcctttccg caacttctta aatgttggtt
     4921 tcttttatac tttctttgta gcaactacaa atttcttcaa atctctaaat gtttgcataa
     4981 caaccgcctt tctgactttt actcaggatg gtatttaaaa atcagcaaaa taaggcttct
     5041 ctccatcttt tttatttcct agctgcttta aagacactct tgctgttttt taatctctat
     5101 caagggggct ctgaatctat tttgatgaac ttatcttatt aatttctcaa atgagctgtc
     5161 agacacaact gtattttttc aatctcatac cggacaacta tgccagctta aaaaaatttc
     5221 ctctggcaaa acttctttat tgaaaaatga caatgaaaaa agaccattct ttttgttgaa
     5281 tgctcattgt gtaaacatat ttcctgcgac tgctcttcga accattctca gtgcaatccc
     5341 taatttatct attaaatctt cttttagttc ctcatctttc atattcaaaa tttcctttgt
     5401 atgttacaca agtttgttct ctaaata
//
LOCUS       DACBZN010000139         5363 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.141, whole genome shotgun sequence.
ACCESSION   DACBZN010000139 DACBZN010000000
VERSION     DACBZN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.141"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(18..1574)
                     /gene="rny"
                     /locus_tag="IVD64_002624"
     CDS             complement(18..1574)
                     /gene="rny"
                     /locus_tag="IVD64_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP6149833.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1985..3034)
                     /gene="recA"
                     /locus_tag="IVD64_002625"
     CDS             complement(1985..3034)
                     /gene="recA"
                     /locus_tag="IVD64_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP6149834.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3143..4384)
                     /locus_tag="IVD64_002626"
     CDS             complement(3143..4384)
                     /locus_tag="IVD64_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP6149835.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4624..5202)
                     /gene="pgsA"
                     /locus_tag="IVD64_002627"
     CDS             complement(4624..5202)
                     /gene="pgsA"
                     /locus_tag="IVD64_002627"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP6149836.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
BASE COUNT         1437 a         1216 c          932 g         1778 t
ORIGIN      
        1 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
       61 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
      121 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
      181 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
      241 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
      301 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
      361 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
      421 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
      481 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
      541 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
      601 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
      661 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
      721 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
      781 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
      841 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
      901 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
      961 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
     1021 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
     1081 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
     1141 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
     1201 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
     1261 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
     1321 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
     1381 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
     1441 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
     1501 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
     1561 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
     1621 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
     1681 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
     1741 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
     1801 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
     1861 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
     1921 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
     1981 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
     2041 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
     2101 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
     2161 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
     2221 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
     2281 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
     2341 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
     2401 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
     2461 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
     2521 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
     2581 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
     2641 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
     2701 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
     2761 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
     2821 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
     2881 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
     2941 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
     3001 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
     3061 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
     3121 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
     3181 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
     3241 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
     3301 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
     3361 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
     3421 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
     3481 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
     3541 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
     3601 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
     3661 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
     3721 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
     3781 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
     3841 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
     3901 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
     3961 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
     4021 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
     4081 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
     4141 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
     4201 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
     4261 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
     4321 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
     4381 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
     4441 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
     4501 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
     4561 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
     4621 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
     4681 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
     4741 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
     4801 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
     4861 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
     4921 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
     4981 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
     5041 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
     5101 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
     5161 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
     5221 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
     5281 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
     5341 gacacagatt tgtcacgatc ctt
//
LOCUS       DACBZN010000140         5339 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.142, whole genome shotgun sequence.
ACCESSION   DACBZN010000140 DACBZN010000000
VERSION     DACBZN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.142"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(62..280)
                     /locus_tag="IVD64_002628"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IVD64_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IVD64_002629"
     CDS             complement(546..740)
                     /locus_tag="IVD64_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149837.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IVD64_002630"
     CDS             2092..3132
                     /locus_tag="IVD64_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6149838.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IVD64_002631"
     CDS             3956..4288
                     /locus_tag="IVD64_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6149839.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="IVD64_002632"
     CDS             4907..5248
                     /locus_tag="IVD64_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6149840.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1769 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaagga
//
LOCUS       DACBZN010000141         5247 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.143, whole genome shotgun sequence.
ACCESSION   DACBZN010000141 DACBZN010000000
VERSION     DACBZN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.143"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(207..1169)
                     /locus_tag="IVD64_002633"
     CDS             complement(207..1169)
                     /locus_tag="IVD64_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6149841.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IVD64_002634"
     CDS             complement(1171..2610)
                     /locus_tag="IVD64_002634"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP6149842.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IVD64_002635"
     CDS             2795..4741
                     /locus_tag="IVD64_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP6149843.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5247
                     /locus_tag="IVD64_002636"
     CDS             5005..>5247
                     /locus_tag="IVD64_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6149844.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1697 a          888 c          867 g         1795 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DACBZN010000142         5238 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.144, whole genome shotgun sequence.
ACCESSION   DACBZN010000142 DACBZN010000000
VERSION     DACBZN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.144"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            133..1107
                     /locus_tag="IVD64_002637"
     CDS             133..1107
                     /locus_tag="IVD64_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6149845.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="IVD64_002638"
     CDS             1089..1550
                     /locus_tag="IVD64_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6149846.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="IVD64_002639"
     CDS             1600..2064
                     /locus_tag="IVD64_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6149847.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="IVD64_002640"
     CDS             2078..2827
                     /locus_tag="IVD64_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6149848.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="IVD64_002641"
     CDS             2827..3657
                     /locus_tag="IVD64_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6149849.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="IVD64_002642"
     CDS             3657..4304
                     /locus_tag="IVD64_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6149850.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="IVD64_002643"
     CDS             complement(4402..5178)
                     /locus_tag="IVD64_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6149851.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1788 a          707 c          942 g         1801 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaa
//
LOCUS       DACBZN010000143         5212 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.145, whole genome shotgun sequence.
ACCESSION   DACBZN010000143 DACBZN010000000
VERSION     DACBZN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.145"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            604..954
                     /locus_tag="IVD64_002644"
                     /pseudo
     CDS             604..954
                     /locus_tag="IVD64_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="IVD64_002645"
     CDS             948..1370
                     /locus_tag="IVD64_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149852.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTNLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="IVD64_002646"
     CDS             2540..3508
                     /locus_tag="IVD64_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6149853.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="IVD64_002647"
     CDS             3525..4049
                     /locus_tag="IVD64_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP6149854.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="IVD64_002648"
     CDS             4425..4706
                     /locus_tag="IVD64_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149855.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="IVD64_002649"
     CDS             4708..5124
                     /locus_tag="IVD64_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149856.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1972 a          894 c          867 g         1479 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct ttaaaaaatg acagataaaa atgcacaaag aactaaaaaa gagaaatcgc
      301 gtaaaatcaa gcattagaag ctagtaacta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactaat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DACBZN010000144         5149 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.146, whole genome shotgun sequence.
ACCESSION   DACBZN010000144 DACBZN010000000
VERSION     DACBZN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.146"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            317..1102
                     /locus_tag="IVD64_002650"
     CDS             317..1102
                     /locus_tag="IVD64_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6149857.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1115..1966
                     /locus_tag="IVD64_002651"
     CDS             1115..1966
                     /locus_tag="IVD64_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP6149858.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2090..2542)
                     /locus_tag="IVD64_002652"
     CDS             complement(2090..2542)
                     /locus_tag="IVD64_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6149859.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3007..3375)
                     /locus_tag="IVD64_002653"
     CDS             complement(3007..3375)
                     /locus_tag="IVD64_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP6149860.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3525..4007
                     /locus_tag="IVD64_002654"
     CDS             3525..4007
                     /locus_tag="IVD64_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP6149861.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4044..5054
                     /locus_tag="IVD64_002655"
     CDS             4044..5054
                     /locus_tag="IVD64_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP6149862.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         1714 a          831 c         1036 g         1568 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag
       61 cttcttcgga aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact
      121 tcttatttct tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga
      181 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
      241 ggtgtacact cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga
      301 aaaaggagtg ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg
      361 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
      421 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
      481 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
      541 acttgatacg gctttagaaa attttgggac actggacgtt tggattaata atgctgggat
      601 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt
      661 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga
      721 taaaaaagga aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt
      781 tgctcattac gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga
      841 gtacgcacca aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat
      901 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
      961 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     1021 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     1081 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     1141 ggcagttggg tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     1201 tgagatatta ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc
     1261 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg
     1321 ggtcatcgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     1381 gccaatttcg actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg
     1441 agaatggtct ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt
     1501 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     1561 aatcagtcta ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact
     1621 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     1681 agtgatttat gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc
     1741 aacaatagtt ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga
     1801 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     1861 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     1921 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     1981 aaatctcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     2041 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     2101 gtgaaatatc tgtctgcatc aataattttt cattgatcca ttgactaaag tttacgacat
     2161 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     2221 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     2281 attgattgat tgctgtaaaa tcacgatact gaaggttgaa acgcatcgtg attggtgcat
     2341 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     2401 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     2461 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     2521 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     2581 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     2641 tcaagtcaga aaattctaac ttttctttct ctaaagaaat tcgttctctc ctaaagtgat
     2701 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
     2761 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
     2821 agtggaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
     2881 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
     2941 tgaacaaaac ctgtataagc tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
     3001 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
     3061 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatctgt
     3121 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
     3181 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
     3241 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
     3301 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatctgat tgatgatcgg
     3361 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
     3421 acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg ctatatttat
     3481 atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt gacttggcaa
     3541 agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc atttatacaa
     3601 atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag accaaaagct
     3661 tattcttaaa agataaaaaa gataataact acgtatatct gacttttact acgaaacaaa
     3721 cggatttcaa agcattgggg aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac
     3781 acatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac
     3841 agattccgat tattattgat gaagagctct tattaaatga aaaacttgtt tttgcaccag
     3901 gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc atccagctac
     3961 ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca agtaaaatag
     4021 tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga aatacaacga
     4081 ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag
     4141 ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg ctttggacgc
     4201 aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat caaatgatgc
     4261 aagatgaaaa tatccaaatg atctttccgc cttggggagg agaattattg attgagattc
     4321 tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat tcagatatta
     4381 gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat ggaatcaacc
     4441 ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa agtgtactga
     4501 aaacaagaga aggagaatcg attctacagc aaccttctga aaagtatcaa aaagagtggc
     4561 cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat tggcaatcag
     4621 tcagcggaga tgcagtaagg atgaagggac gattattagg tggctgtatt gatacgatca
     4681 gtcatttaat cggtacccct tatggagatg tccaatcttt taaaagaaag tataatgatg
     4741 gaaagccgat cttgtggttt ctagaaaatt gtgagctatc gtcagttgat ttaagaagat
     4801 cacttgttca gatgaaactg gcaggatggt ttgatggcag ctcggggatc atgttcggta
     4861 gaagtgcagc agaggatttg ttagattata aaatgaagga tgtatacgaa gatctgtcaa
     4921 aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg ccacaactga
     4981 cattgatcaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg actgttttgc
     5041 aaacgtttat ttaaaaaatt ttttcctgcc ttggagttta tcaaagacca acaaatctaa
     5101 atagaaaaga aaatgaaaga agctgacagt attttgccag cttcttttt
//
LOCUS       DACBZN010000145         5131 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.147, whole genome shotgun sequence.
ACCESSION   DACBZN010000145 DACBZN010000000
VERSION     DACBZN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.147"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..261)
                     /locus_tag="IVD64_002656"
     CDS             complement(<1..261)
                     /locus_tag="IVD64_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6149863.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELE"
     gene            complement(274..1599)
                     /locus_tag="IVD64_002657"
     CDS             complement(274..1599)
                     /locus_tag="IVD64_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP6149864.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1616..3028)
                     /locus_tag="IVD64_002658"
     CDS             complement(1616..3028)
                     /locus_tag="IVD64_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6149865.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3045..3809)
                     /locus_tag="IVD64_002659"
     CDS             complement(3045..3809)
                     /locus_tag="IVD64_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP6149866.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(3913..4788)
                     /locus_tag="IVD64_002660"
     CDS             complement(3913..4788)
                     /locus_tag="IVD64_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6149867.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5043..5127)
                     /locus_tag="IVD64_002661"
     tRNA            complement(5043..5127)
                     /locus_tag="IVD64_002661"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5091..5093),aa:Leu,seq:caa)
BASE COUNT         1559 a         1081 c          773 g         1718 t
ORIGIN      
        1 ttcgagttca actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc
       61 gatcgttatg gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg
      121 gatatatcca tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac
      181 ctcgtctttt actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt
      241 tcttgtttta aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt
      301 gtttactttg atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg
      361 attcaatgta aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag
      421 taacttctct actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt
      481 tcttagtgta attggctcca caccagttgc accaacatat gccggtattt caacaactgc
      541 atctgaccgt agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg
      601 atcatcattc aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt
      661 aaagttcaat acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt
      721 tttctctcgt ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac
      781 gaccatatca ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt
      841 tttcgtgatg actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa
      901 atcggcgact tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg
      961 ttgcaattct ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt
     1021 aacgatccaa ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat
     1081 agtcatatca caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga
     1141 aacgatcgac tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat
     1201 atgatcgaca atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag
     1261 tccacacgtt tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg
     1321 catctccaat cctccaacac gaatttgtga gaaaacaaaa tcgcgtcctg taaaagcaag
     1381 ctttggatca tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa
     1441 tttaacaatc aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc
     1501 agataatggc aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt
     1561 actcccgcct ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt
     1621 cttctaataa tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt
     1681 gataattttt ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt
     1741 cctcgaccat ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat
     1801 ccaggatatt ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat
     1861 ttttacttgc taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct
     1921 tagcttcacg tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt
     1981 aatacaaggc aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc
     2041 gaaaattgat gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga
     2101 ataagtaaca gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag
     2161 gagaaagaaa catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc
     2221 ctgcaccgct tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat
     2281 agatagctag acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa
     2341 acagtccagc ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct
     2401 tgctaggaga tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca
     2461 gtccaaaggg gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca
     2521 tatcaacgag cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc
     2581 ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc
     2641 ccgcaaaata agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg
     2701 ccgctactaa accggaaata attccaccaa aaatccccat attcacttct gggtccataa
     2761 tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt
     2821 tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc
     2881 caaaaacggc atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata
     2941 aacgcatgat caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa
     3001 gcttctgcac atttgattta acattcattt ttattcctcc tctattaaat atcctcatat
     3061 gttactaact caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa
     3121 aattcgactt ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc
     3181 aaatacgctg cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa
     3241 cttgccaaat attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct
     3301 acgacatcga aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag
     3361 ttgctccgaa ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca
     3421 tccaaagtat gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa
     3481 attttttgaa tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga
     3541 tgctgataat aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg
     3601 aggagtggtt tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc
     3661 tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc
     3721 aaaattcctc gctgataagc atccataatc ccataattca caccatggct atagccaaaa
     3781 tcatccgaat taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag
     3841 gcgcaaccaa ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt
     3901 tacgccctta ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc
     3961 ttgaatggca agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata
     4021 cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc
     4081 ctgataaatc aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag
     4141 tgattcttta tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt
     4201 catttctact atttttaagc tctttaaact attaggctct cctagctcag cattaatgat
     4261 atgcatagtg atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat
     4321 tcgctcgacc acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg
     4381 caacataatt gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg
     4441 atctgtcaat gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag
     4501 ttcaatcact tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact
     4561 gaatcgtttg atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc
     4621 aaatggctta ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat
     4681 tccatcgtca tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa
     4741 ttcacctcct ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt
     4801 ctccaaacaa tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc
     4861 ctaattgaat agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg
     4921 ttatcaatat cttttttact cgttttatga taatacttgt atcgaattga tttggaggta
     4981 attacataaa aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat
     5041 tataccggcg gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag
     5101 cgcgtctgcc aattccgcca cgccggcaaa t
//
LOCUS       DACBZN010000146         4936 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.148, whole genome shotgun sequence.
ACCESSION   DACBZN010000146 DACBZN010000000
VERSION     DACBZN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.148"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            119..712
                     /locus_tag="IVD64_002662"
     CDS             119..712
                     /locus_tag="IVD64_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6149868.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            728..1627
                     /locus_tag="IVD64_002663"
     CDS             728..1627
                     /locus_tag="IVD64_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6149869.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            1630..2115
                     /gene="ssb"
                     /locus_tag="IVD64_002664"
     CDS             1630..2115
                     /gene="ssb"
                     /locus_tag="IVD64_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6149870.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2481..2741
                     /locus_tag="IVD64_002665"
     CDS             2481..2741
                     /locus_tag="IVD64_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149871.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3182..3388
                     /locus_tag="IVD64_002666"
     CDS             3182..3388
                     /locus_tag="IVD64_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6149872.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            3388..3639
                     /locus_tag="IVD64_002667"
     CDS             3388..3639
                     /locus_tag="IVD64_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149873.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            3654..4091
                     /locus_tag="IVD64_002668"
     CDS             3654..4091
                     /locus_tag="IVD64_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149874.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            4084..4791
                     /locus_tag="IVD64_002669"
     CDS             4084..4791
                     /locus_tag="IVD64_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149875.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
BASE COUNT         1802 a          821 c          985 g         1328 t
ORIGIN      
        1 aagaaccagc agcggattca aaaagaaatc gaattagatc gataaattcc tttcttgtta
       61 atataaaact aacatgttat agttgtgtta gttttatatt aactttaggg gaagagtaat
      121 gaaagacaaa atccaatgta tttttgataa acataatggc aacctatcct tgaaggatgc
      181 tagaagtgaa ggattatcgc ctatgacgtt agatcgatta gccgaaaagg ataaaataga
      241 aaaggtagca ccaggtttct ataccttaaa ggatgcgttc gtggatgaat tgtatctcgc
      301 tcagagtatc tattccagag ggattttttc tcatgagact gcgctcgatc tttttcaagt
      361 aggaacatat atccctaaaa agatcaatat gatgtttcct aaaggataca atgtatcaaa
      421 ggagaaatta gatcagtatg ccattcaacc gcattacact aataaagaaa attttgacct
      481 gggagtcaca gaaacagaaa gcttttacgg caataaaatt ttagtctatg atttagagcg
      541 aacgttatgt gacatgtgga atccaagata caaagcaagc gtagaggtaa aacaagaagc
      601 attaaaagaa tatatgacaa gtaccagtag aaatacaaat aagctaagaa gatacatgga
      661 tattttaaaa tcaccaaaag aaatgacttt gtatatgttg ccactttatt gagaggagta
      721 aagatgaatg acaccgacac aatttaaaga gaaaacaaga aatttcgcag cagagaaaca
      781 tttaaaccca caaattgtcc aaagatatta tatgatggaa aaattattag aatgtattgt
      841 tgaatcgcct tatcatgatg attttgtatt aaaaggggga tttctgattg gatcaaaata
      901 cgggttagaa aaccgttcaa ccgttgatat tgatacgact tatcgaaatt cgaaactaac
      961 aaaagaaaag ttagaaactg ttttttctga attaacagct aatcctacaa aagatggaat
     1021 tcgttttgaa ttaaagggat taacagagac gagggaagcc gattattatc caggttttca
     1081 agcaaagttt attgcttatc ttgaaaatgc acgtgtacct tttaaattag atattacttc
     1141 aggtgattct attattccag cagcattgat gtatgaacac aaattaatgt ttgaagataa
     1201 aacgattcat attccagctt atcctacgga acaaattcta gctgaaaaat tacatgccac
     1261 gtttagtttt ggaaaagata attcgcgcat gaaagatttt tatgacctgt atatgattcc
     1321 gaaattagaa cgtatcgatg agaaaaattt gtataagagt gttcaaggaa cattcaagga
     1381 aagaaataat caatcggtgt ttaagcattt gtatgatcag gaaatgcctc aattggtgaa
     1441 agatgagggc ttgagagaaa agtggaaaag ataccaacaa gaaaactatt ttgccaaaga
     1501 catttctttc gaatctacac taacttccgt tgattccctg atgcaatcaa ttgtagagca
     1561 agagaaggca gaacgaaaac aaaattttaa gagatcacaa caaatgaatc aagaaatgga
     1621 gagataaaga tgatcaataa tgtaacttta gtagggcgat taactaaaga tccagattta
     1681 cgttataccg caagtggcac agcagtggcg actttttccc tagccgttaa tcgtaatttc
     1741 acgaatcaaa acggcaatcg agaagctgat tttatcaatt gcgtgatttg gcgcaaacca
     1801 gcagaaacga tggcgacatt agctaaaaaa ggcagcttga tcggcgttgt cggacgaatt
     1861 caaacacgga cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc
     1921 gataacttcc aactattaga aagtaaagca gcgacagaaa gccgtatgca cgctgatcaa
     1981 tcgtccagca gtccaagccc taccaccttt gaacaacggg atacagcgac gcctaacaac
     2041 aatggattga acgcttcaca gaatccattt ggtggacaat caatcgatat tagcgatgat
     2101 gatctaccgt tctaagtagc attttctatt ggttgcattt gctacggttt atgttataat
     2161 ttagacaaca aaaagaattt tcaatctata accaaaaacc actagctggc gggctagtgg
     2221 ctttctttta cgtttctatt ataagaatta acgggtaggc ttactgtctt tcttggcaaa
     2281 tttacaatca agccatttgc agatatagtg cgcggctact tctgccatga ttgcaaaaac
     2341 aaaatcaaaa agaattttca aacctataac ctcctcccct tgccagatta ggccggggta
     2401 gcagacagca agactattat agcataattg aaatatccaa atgattggat ttttgctata
     2461 attttagcga ggtgattgtg atgaacaaat atgttggttt gcttgataaa atccgagtaa
     2521 taaaaacgca accgttattg gttcgcttta ctttacaaac gattcatgaa tcgattaatt
     2581 gtgtagttgc agatattgaa attatcgata aattgctaat tatggatgat ggaaaataca
     2641 atattgccgt cacaggtcac ttcaataaac gaaatcagct agttattgct agcatgtatg
     2701 ttagaaatcc cgatcatttt acgcgatcga tgggcatata aataaaagaa gagttgtagt
     2761 cctaaatgag gattgcagct ctttttgttt tacatagaaa caggaaaaga cctttttagg
     2821 aagacacaag tgccttctca attgaaaaac ctttctctca ctccaaccgc caccgccgtt
     2881 cgagaagaaa ggaagtgaac gttgcacgtt cactaagata tggtgtcttg caaagcaaga
     2941 caaaatagaa aaaacttcct ctcaccaaaa ggttcgttcc tgttcgctat ctctttattg
     3001 atttgccaga ccggcaacaa gttgcctttt atcaccaaaa agcgacaagg acaagggtaa
     3061 atggcacgct gcgcgccctt gtccttgtcg cttttcgttg attcattctg gccaacaaat
     3121 caataaagaa agaactaacg aaaggaaata aaacctttcc taagaggagg cagaatcaat
     3181 catgcgaaag aaatttgatt attggggtgt tcccttcagc gaattattcc caaactatca
     3241 tgcaccacat actgtcgaat gcgattgtgg agaaagagcg aaatgtatca aaagctatcg
     3301 tttgtatcaa tgtccaacat gcgggaaaaa atataccttg tcttatggcg attatgtttt
     3361 gattgatgag aaagtaaaga aacggtgatg aaaacacaac acaaattatt gactgtaaaa
     3421 gaagtcgttc aaattttggt ggacaaagaa gtcatttctg aaaaagtagc agaagcttta
     3481 cttattccgc ggtactggac gatagaaacc cctttagtag aggatgcacc gactatgcta
     3541 tttgaagacc tagtgaagat tgcaggatat gaactgcatt atcaagaaaa catggaaact
     3601 tatgaaatcg aatattggtt aaccaaatta gagaactaaa aaggagaaaa aatatggctc
     3661 aaaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa aaaatggagg
     3721 atatgacgtt cctataccat gaaacacgtg tacccaaaaa acattacaag gaaaaattgt
     3781 cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt gcgacgtact
     3841 atcgaacact ggaagaactg aaaaaacaaa atggaaaatg gtttttccaa gccttactat
     3901 gcttggaagt aggtgtcaaa ccaagcacta tcaaaccttc cgaatatcaa gccttagaac
     3961 tgacttatgc aaaattcgca cagattaaga aagctaaagc aatggctagc gactggctag
     4021 atacttttga aaatatcgaa aaaaatggtg cattttatac gattgaatcg gaggagaaag
     4081 ataatgaata aggtggttta tcagctaaca acagaagatg tttcgcaatt tttaacaaac
     4141 aaagaacact tgaatgaagt aatcagacgg attcaaaaag actttagttg tatggaagaa
     4201 atgaagtact ttattcaaag agtggaagaa cagttgtata tggaaagtga aacgagtcaa
     4261 acgaaggtta cagtcagcca taatcgttta gacgtgtata aacgtcgtcc tgacacatta
     4321 aaaaaaggag cggagcttct acgactagaa acagaaaaat atatcatgaa tgtttaccta
     4381 gaaaggagtg agctggtcga aaactttgtc agctacacga tttgcaggaa acactattac
     4441 tcagaatcag aatattattc aatttttgag agcatggaat taatggaaga agaaatggaa
     4501 gagctaaaca gcaaagatgc gacggtagac gattattacc ttgtatttac tagatttttg
     4561 acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcac tgtacccgaa
     4621 gcatttttga gggaatatgt agaagaagta gataacccag gcgacgactg gtacacgact
     4681 ttaactgatt ttttagacac ctacacttac gacgatacag aagaaatcca aaatgaactt
     4741 gaccgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtcaata accatttata
     4801 ccgaaaagtg aggatgtgtg aaataagaac aaaaaacctt gaaattaagg gaatggttct
     4861 tatttcgcac caatcgatcc aaccgccccc gccgcttagg tggtgcgaaa taagcgggca
     4921 aagcccgata acctaa
//
LOCUS       DACBZN010000147         4893 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.149, whole genome shotgun sequence.
ACCESSION   DACBZN010000147 DACBZN010000000
VERSION     DACBZN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.149"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..563)
                     /locus_tag="IVD64_002670"
     CDS             complement(<1..563)
                     /locus_tag="IVD64_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP6149876.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IVD64_002671"
     CDS             complement(750..1400)
                     /locus_tag="IVD64_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149877.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IVD64_002672"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IVD64_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP6149878.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IVD64_002673"
     CDS             complement(2912..3910)
                     /locus_tag="IVD64_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP6149879.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IVD64_002674"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IVD64_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP6149880.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
BASE COUNT         1486 a         1051 c          757 g         1599 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tat
//
LOCUS       DACBZN010000148         4680 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.150, whole genome shotgun sequence.
ACCESSION   DACBZN010000148 DACBZN010000000
VERSION     DACBZN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.150"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(92..943)
                     /locus_tag="IVD64_002675"
     CDS             complement(92..943)
                     /locus_tag="IVD64_002675"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP6149881.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(1097..3670)
                     /gene="mprF"
                     /locus_tag="IVD64_002676"
     CDS             complement(1097..3670)
                     /gene="mprF"
                     /locus_tag="IVD64_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6149882.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3811..4617
                     /gene="proC"
                     /locus_tag="IVD64_002677"
     CDS             3811..4617
                     /gene="proC"
                     /locus_tag="IVD64_002677"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP6149883.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         1462 a         1026 c          809 g         1383 t
ORIGIN      
        1 ctcatatcta gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca
       61 gtttttcatc acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt
      121 cctcacaaaa accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca
      181 aggcaaaaac ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca
      241 tacgctcttt gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt
      301 ttcccatttt tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt
      361 ggatgtcttg atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga
      421 cactcattct tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga
      481 tttcttcccc tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg
      541 taccgacttc caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt
      601 ggttgatccc tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta
      661 ctggaatatt cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta
      721 gcaataaagc agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg
      781 tttctacaat gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta
      841 cgctcgacat caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca
      901 gtcctaaatt tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa
      961 aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct
     1021 agtggttgat tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa
     1081 caagttctag tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc
     1141 ctttttcttt tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt
     1201 tcctataagc aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag
     1261 cagactggaa ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt
     1321 gaaagaaaga aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt
     1381 ccgagtagcc cattttctcg tatacaataa acagattggc taacagatag tcagctaatt
     1441 cctcagattc tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg
     1501 gctgagcggt gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag
     1561 ataatacata tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa
     1621 gccatttttg tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc
     1681 aagtaaaatg gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag
     1741 acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac
     1801 ccaattcatg aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc
     1861 ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc
     1921 caactggatc tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt
     1981 gatcttggtc gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc
     2041 ttgtagctgt gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac
     2101 tgccaagctt cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg
     2161 agatgcccaa accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat
     2221 gatttcctgt tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa
     2281 cgataaacaa aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga
     2341 attttgttcg ataaaattct tttctggata accacacagc taacagaatc aagacatata
     2401 ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta
     2461 aagcaatcgt aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga
     2521 gtaataaaaa gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca
     2581 ataatacttg aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca
     2641 tcatgatccc cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa
     2701 acagtcgcgg aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca
     2761 atagaagtcc cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa
     2821 ctactgctac atcttgccga acagacaatt gactcattcc taagatcatc aaaacgtcaa
     2881 aagtccccat tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca
     2941 taggatagat cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata
     3001 ccgcaagggc tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg
     3061 gataaaactc gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg
     3121 ctaaaaaact accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa
     3181 taaaaaagac atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata
     3241 gtgcgacttt tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac
     3301 tagcccgcag acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg
     3361 ttacccacgc agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta
     3421 agactctgat cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca
     3481 gaatcatgag caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc
     3541 aactcatgcc attagcaata tttgcgactt gatttgccac aaagactaac acggagccga
     3601 aaaaaatcag ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt
     3661 tatttttcac attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc
     3721 ttatttattt taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa
     3781 agatatccta atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat
     3841 atggcacaag ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg
     3901 ttcgtttcca gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat
     3961 gcatgcaaaa gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa
     4021 ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc
     4081 atttctattg cttcaggaaa atcattaaca gatttagaaa cagcgcttga agatcctcag
     4141 gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg
     4201 atttgcaaaa atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa
     4261 gcaatcggca gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct
     4321 ggcagctcac ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg
     4381 aatggtttgt ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt
     4441 caaaaattcc tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct
     4501 ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc
     4561 atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga
     4621 tagcaatgtt ttttcgtacc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt
//
LOCUS       DACBZN010000149         4657 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.151, whole genome shotgun sequence.
ACCESSION   DACBZN010000149 DACBZN010000000
VERSION     DACBZN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.151"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            252..527
                     /locus_tag="IVD64_002678"
     CDS             252..527
                     /locus_tag="IVD64_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149884.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            540..2618
                     /locus_tag="IVD64_002679"
     CDS             540..2618
                     /locus_tag="IVD64_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6149885.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..>4657
                     /locus_tag="IVD64_002680"
     CDS             2781..>4657
                     /locus_tag="IVD64_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6149886.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     V"
BASE COUNT         1552 a          746 c         1005 g         1354 t
ORIGIN      
        1 gtcgcagccc catttttttg ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa
       61 acatcctttt gtcagaaact tttatctttc ttaagtttta cttgatatct gatactattt
      121 ggaatagaat gaggcgttga aaaatttatg gaaatttcta actagcaata aacgatgaaa
      181 caatgaaata tttttcatcc aaaaagaact ttgctataat gatggaagtt tgtgtatagg
      241 agatctggaa aatgagagag agttttgaac aacaaaaaaa actgttgcat gatcgctatg
      301 gcgccctttc gatggatgac cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat
      361 tgatgtatcg tcagttggaa cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg
      421 tacagtgtga attagagggg aaccaaacac aggttgaagt agtcgaaacc aaaattttaa
      481 agaaaaaaga acaattttta aaaatgctta cacaaaataa aaaataaagg ggatggacga
      541 tggaatttgc tgagttagtt attgtatttg cagtaacgat caccttatcg aacatagcta
      601 gccgcatttt gccaatgatt ccagctccac tgatccaaat tttccttgga gtggtattag
      661 gtctgacaga gtggggacaa tcaatcgatt ttgaacctga attgttctta gttatgatta
      721 ttgcaccatt gttatttaga gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt
      781 tcgggacaat tttgttcttg gcatttggag gagtcatttt gacccttgtc ggggtaggtg
      841 cgacgttatc attcctgttg ccaagtgtcc cacttgctgc ctgctttgct tttggagcag
      901 cgttaggtcc gacagatgcg gtagcagtca gttctctttc tggacgagtg aatatcccta
      961 aaaaggcgat gcatattttg gaaggagaag gtcttttgaa tgatgcatct ggtgttactg
     1021 ccttccagtt tgctcttggt gcgctgatca ccggatcttt ttcagcagta aacgcgggga
     1081 tgagtctagt agtttctagt attgggggag ctttgatcgg ctttttgctt gtctggttca
     1141 aacaaaaaat aattcatttg atcgaaaaag catcagcaca agatgtcaca gggtatctgc
     1201 tgatcgaact tttgcttcct tttctagcct atgtattggc agaggcattt gatgtatctg
     1261 gaatcattgc tgcagtggca gcaggtattc tccaagcttc tggttttagg aaaatctctg
     1321 tgtttgatgc agaattgtcg agcttgtccc acagtacatg gacaactatt gcttttacac
     1381 tgaatgcatt agtatttata tttttaggaa tcgaattgac gcaagtgttc tcgccagtat
     1441 ggggagacgg tttatatcca aacggtcttt tattagcgat tatcgtatta atctctgtca
     1501 tgctttttgt gatcagattc atttctatca gtctttttta tgtattcaaa gatggcagta
     1561 aaaagttcaa aaaacagttg aacgagatct tgatcttaac atttggcggt gtaaagggaa
     1621 cagtcagttt ggctacgatt tttattttgc cattttcaat caataatatg atgttttacc
     1681 agcggtcttt gcttttgttt ctgacggcgg gtgtcatctt agtgactttg gtcataggga
     1741 tcattgtctt gcctatgctg acggaaacag aggaagcgaa aagcacggat ttgaatgctt
     1801 tgatgatttt agaagaggtc gttgaagttt tgcgaaaaga aataaaggaa attgatcgta
     1861 atacaaaaga atttttagcc actgaagcag tgatagaaaa ttatcaagaa cggatacgtg
     1921 atctgtactt ggaagattta accgatgatg aaaagcaaga agtacaagaa atacaagcac
     1981 tgattttgtc gatcgaacgg gatggattgg atgaaagtta tcgttcgggc aagctttctt
     2041 ctaatggtta tcgtttttac tcccgttttc tttctcgatt cgaacattct atcacaagcc
     2101 agattttgtc ttttatcggc ttttggttca ttgttgtccg taggttaatg cgaatcgttc
     2161 tccatccgaa aatgttttgg caaagaagat atgctgatcg tcagacattt attagtgatg
     2221 aagatatcca agaaatcaga gagacatatc aaaaaaatac tcagttgatt attgaaagtt
     2281 tagataactt gactgatatt tatgatgaaa cgatggttca attttttatc cagcaacgca
     2341 aacttgaagg aataaaaatg aactcgggga atttgatttc ttcgtggatg atccagcaag
     2401 atagtctgtt tacgaaaaaa atgctaagag gttattatct tgaaagaaaa atcattgatg
     2461 aatacgaagt tgctgaaaaa atcacgacgt tttctgcgaa cagctatcgc aggaatatca
     2521 atttgttaga atcttatacg atgaacaaac catctgataa cttttctttc cgatttgcgt
     2581 ttcgagcaaa gtcaaaaaaa acgagtccaa taaaataaaa aggtaaaaat tttcaagttt
     2641 tataaaaaac aaaacatatc agaacaggat cgaatcggtc ctgttctttt tttgagaaca
     2701 caaaaagaac aaataccaaa aaaattttcc tgaagttcac tatttttatg atacaatatc
     2761 caagataagg agggaacatg ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg
     2821 gtgaaaaaga tttgtttgac cagatttctt ttttggtcca cgaaaaagac cgtatcgggt
     2881 tgattgggac gaacgggacg ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata
     2941 gcagcgacgg tgatcggcaa acagtgtttt atccaactga ttaccgtatt ggttatcttt
     3001 ctcaagcagc tgaattttcc gatgaattaa ctgttctcca agcagtcttt caaggaaaca
     3061 gccccttgat ccaaactgtt cgagcatatg aggaagcatt gatcgaacta ggggaaaacg
     3121 gagaagaccc tgatgtgcaa aaacgttatg ctaaagcaga agaacaaatg aataaagaag
     3181 atgcttggac tacagatacc aacgcaaaga ttattttgca aaaattagga atcagtgaat
     3241 tagataaaaa aatcagtact ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag
     3301 tgttgattga tgaacccgat ttactattac tagacgaacc aacaaaccat ttagattatg
     3361 aagcgatcga gtggttggaa aactatttga aacaataccg tggcgcacta ttgatggtta
     3421 cccacgatcg ttattttcta gatagagtag caaaccgaat cttcgaactt tcatttggaa
     3481 aattatacga gtataaagga aattatgaag cttacgtctt agaaaaagca gaacgtgatc
     3541 gtgtagaagt agaacaagaa gaaaagcgca agcggcttta taaacaggaa ttagcttgga
     3601 tgagggctgg cgtacaggca cgtggcacca agcagcaagc acggatcaac cgtttcgaag
     3661 atctaaagga gaacttgtac caagtaaacc aagaagatga tttagagctg aacctagcga
     3721 cacaacgttt agggaaaaaa gtactggaaa tcaaagatgg cagttaccgt ataaatgacc
     3781 aaacactgct agaacattta gatttattga ttcaatctcg ggaaagacta ggtattactg
     3841 gtaaaaatgg tgcgggaaaa tcgacattgc ttaatattct agctggcaga attcccctgg
     3901 atagcggtac aatgtctatc ggtgaaacgg ttcatcttgc gtattataca caagaaaatg
     3961 aagaaatggc accagataaa cggatgattg cttacttaca agaagctgct gaagaagcaa
     4021 aaacagcaga tggcagtcaa atcggagtag cagaattact tgaacgattt ttatttccaa
     4081 gatttatgca tgggacactg ataggcaaac tttcaggcgg agaaaaacgc cggttatttt
     4141 tgttgaaact gctgattcag caacctaatg ttttattatt ggatgaaccg accaatgatt
     4201 tggacatagc aacattgacg attttggaag attattttag aagttttcca ggtgctgtga
     4261 tcactgtttc ccatgaccgt tatttcttgg acaaggtagc agataagtta ttagtattcc
     4321 aaggaaacgg aaaacaagaa ctttattacg gaaacatgtc cagttattta ctgaagcaaa
     4381 aagagaccca gcagcctgct gaaaaagcga aaccaaaaat acagagcaag gaacctgctg
     4441 aaaagaagaa attatcttac atggaacaaa aagaatggga aacgattgaa gatgagattg
     4501 cagaacttga agaaaaaatc tcattgctcc aagaagagat gaatcatcaa ggagataatt
     4561 tcactcgatt acaagaattg caaaacgacg tatctgaaac agaagcacaa cttgaagaaa
     4621 aaatggcacg gtgggaatac ttgagtgaat gggtaga
//
LOCUS       DACBZN010000150         4651 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.152, whole genome shotgun sequence.
ACCESSION   DACBZN010000150 DACBZN010000000
VERSION     DACBZN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.152"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(162..464)
                     /locus_tag="IVD64_002681"
     CDS             complement(162..464)
                     /locus_tag="IVD64_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149887.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(684..1050)
                     /gene="ssrA"
                     /locus_tag="IVD64_002682"
     tmRNA           complement(684..1050)
                     /gene="ssrA"
                     /locus_tag="IVD64_002682"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1221..1994)
                     /locus_tag="IVD64_002683"
     CDS             complement(1221..1994)
                     /locus_tag="IVD64_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6149888.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2138..2488
                     /locus_tag="IVD64_002684"
     CDS             2138..2488
                     /locus_tag="IVD64_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6149889.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2469..3185
                     /locus_tag="IVD64_002685"
     CDS             2469..3185
                     /locus_tag="IVD64_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149890.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3185..4633
                     /locus_tag="IVD64_002686"
     CDS             3185..4633
                     /locus_tag="IVD64_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6149891.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
BASE COUNT         1547 a          822 c          897 g         1385 t
ORIGIN      
        1 agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat aaaatataga
       61 tatcatcata ttacacaatc ttaatactaa cttaaaaaat atccctttta taagtatggt
      121 gataaaatcc gttccgggct acctttttta ggtagcctac tttaatcttt atacctttct
      181 ggatcaacga aagtatactt caaatagtca taacgccgat gatcgctccg tgcgtctggc
      241 acatcagtca cgatatcaaa caaaaaatac acatctttct tcattctggt ttttgttgcc
      301 ggaattttaa agtagttctt attagagtaa tagagattga ttaataagct atcttcgatt
      361 gctaaaaaga aaacttctga atcccacacc ttataaaaat ctttgacaaa tctattcgaa
      421 ggatcaaatt taaaccataa ttgtgttttt ccttccatca acataatgtt cacctcaaaa
      481 agagtatacg aactaatgtt ctttttgtaa agtaggaata tcattgataa tatggatgga
      541 acatatatat taaaaagtgt tctttttgaa tgctattttg aatgccattt tcattcattt
      601 aacgaaaatc tgcgaaaata aaaaatgctc aaacccttat aaaataagga tttaagcatc
      661 tagcgaaatc tgctgaaatc tcacaatgga gacggcggga gtcgaacccg cgtccaaaca
      721 catcgccact taaaaatcta cgttcatagt caactcattt aaggattcac tttacagctt
      781 gccgagtgac aggcattctg tatcgctagt ctgatgttct ctttttcaac ttacagacgg
      841 aaagttgaaa cgtatcccac taaagttagg acccttactc gagcacatgg gcgatgccga
      901 gaggatctac gctaactgtt tttaggcagc taaagcgtaa gagttgtttt cgtttttagc
      961 agttatattt aactgtaacg tttttacgta gacgtaacct acgaaacgca attcaagctc
     1021 gactgtgcct gtcgaatccg taacgtcccc gttagctaac aagggttacg tgtatttttc
     1081 atacaaaatt agtatagcat atattgggat aaattcaata aaaaagcgaa tcatatccca
     1141 ttatttatat aataactgcc cctttcttaa gaaaaaataa gcgattttct taagaaaaga
     1201 ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg gacaattccg
     1261 ccttctgtat ttttctttgt gataaaggta gaaagttctt ttaatccgtc ataggcgttg
     1321 cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg accatcgcca
     1381 aatccaaaag taggaacttt ttccagctga agttcttttt gcagacgttt gacacctgcc
     1441 ccttttgaga ttcctttctt cactgtatcg atcgagtaag gaccattccg ataaaaagtt
     1501 agctcaggaa aatgttcgtg atagtattcg tcacctgatt gagataagat cagcaacata
     1561 tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga atgaatgaag
     1621 tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg atcgttatag
     1681 taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca ttcttctgaa
     1741 gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc attcatggtg
     1801 accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc tgtacgtcct
     1861 gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt tgccacttcg
     1921 ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa agtgattgca
     1981 cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct taacaaagaa
     2041 tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag tcaacaattt
     2101 agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca gtattggtat
     2161 cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa atctgccttt
     2221 ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc tggctcaaaa
     2281 gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg gctattattt
     2341 aaagacatct gggcatacga attattttct gattgaaatg cttctgctag tatttccaat
     2401 tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg aaactggagg
     2461 gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa atgatgcaga
     2521 tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa gaagggctcg
     2581 atgccttttt agaaaaccca caacattggg atatgattac actagacctg aatttgccca
     2641 cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact gtgccgatta
     2701 tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag atgggggcag
     2761 atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg aaagctttgc
     2821 atcgccgtag tgagttggca gaagcaagag aagaagcagt agtttcagat gatcattttg
     2881 atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat ctggacaacc
     2941 ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta gcaaaaaaac
     3001 cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat cagtactttg
     3061 gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc gaaaaggtcg
     3121 gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat tctggtgttg
     3181 cctaatgcgt tatttgtatc agcaactctt agctttctgc ggaatggtcg cgttgatctt
     3241 attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag aaaacaatta
     3301 ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct atgccgatac
     3361 gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga cagagcaagt
     3421 actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg tcatttatcc
     3481 gactaataca gctttactga attttacctt aacagctgat caatggaaaa atctgaggaa
     3541 tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac aggcgacctc
     3601 ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg tagttagtca
     3661 acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt tcaaaggatt
     3721 tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat tccaagttaa
     3781 gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt ttgatgtaca
     3841 gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca ataaaatgac
     3901 gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc gaagacaatt
     3961 tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg gacttctgga
     4021 agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta aattgatgaa
     4081 aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg aaaaaatccg
     4141 tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat tgaaaaatat
     4201 tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat tgaaagccgt
     4261 tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg tcaatattat
     4321 tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag aaaaaggcta
     4381 tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac aacaaatgaa
     4441 aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa aatatggtga
     4501 atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag gaaaattaga
     4561 agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg acgtagaaat
     4621 caaaaaagag taaaaaacaa gcggtataaa a
//
LOCUS       DACBZN010000151         4618 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.153, whole genome shotgun sequence.
ACCESSION   DACBZN010000151 DACBZN010000000
VERSION     DACBZN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.153"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(15..962)
                     /locus_tag="IVD64_002687"
     CDS             complement(15..962)
                     /locus_tag="IVD64_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6149892.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IVD64_002688"
     CDS             complement(1140..1943)
                     /locus_tag="IVD64_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP6149893.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="IVD64_002689"
     CDS             2089..3003
                     /locus_tag="IVD64_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6149894.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="IVD64_002690"
     CDS             3051..3521
                     /locus_tag="IVD64_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP6149895.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="IVD64_002691"
     CDS             3606..4214
                     /locus_tag="IVD64_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP6149896.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="IVD64_002692"
     CDS             4348..4548
                     /locus_tag="IVD64_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6149897.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1512 a          716 c          912 g         1478 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttagat
//
LOCUS       DACBZN010000152         4431 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.155, whole genome shotgun sequence.
ACCESSION   DACBZN010000152 DACBZN010000000
VERSION     DACBZN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.155"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(543..2048)
                     /locus_tag="IVD64_002693"
     CDS             complement(543..2048)
                     /locus_tag="IVD64_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6149898.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVD64_002694"
     CDS             complement(2045..2731)
                     /locus_tag="IVD64_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149899.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVD64_002695"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVD64_002695"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP6149900.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat a
//
LOCUS       DACBZN010000153         4427 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.156, whole genome shotgun sequence.
ACCESSION   DACBZN010000153 DACBZN010000000
VERSION     DACBZN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.156"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            690..1658
                     /locus_tag="IVD64_002696"
     CDS             690..1658
                     /locus_tag="IVD64_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6149901.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IVD64_002697"
     CDS             1669..2484
                     /locus_tag="IVD64_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP6149902.1"
                     /translation="MKVLGLGDNVVDKYEYLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IVD64_002698"
     CDS             2552..3262
                     /locus_tag="IVD64_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6149903.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4427
                     /locus_tag="IVD64_002699"
     CDS             3298..>4427
                     /locus_tag="IVD64_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP6149904.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKK"
BASE COUNT         1492 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaatatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaaaaa
//
LOCUS       DACBZN010000154         4417 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.157, whole genome shotgun sequence.
ACCESSION   DACBZN010000154 DACBZN010000000
VERSION     DACBZN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.157"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            217..492
                     /locus_tag="IVD64_002700"
     CDS             217..492
                     /locus_tag="IVD64_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149905.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            534..956
                     /locus_tag="IVD64_002701"
     CDS             534..956
                     /locus_tag="IVD64_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149906.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            975..2567
                     /locus_tag="IVD64_002702"
     CDS             975..2567
                     /locus_tag="IVD64_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149907.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2579..2914
                     /locus_tag="IVD64_002703"
     CDS             2579..2914
                     /locus_tag="IVD64_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149908.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            3061..3255
                     /locus_tag="IVD64_002704"
     CDS             3061..3255
                     /locus_tag="IVD64_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149909.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            3287..>4417
                     /locus_tag="IVD64_002705"
     CDS             3287..>4417
                     /locus_tag="IVD64_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149910.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHS"
BASE COUNT         1809 a          645 c          888 g         1075 t
ORIGIN      
        1 atcactttct cacgaaaggt gttaggtagg ctgtgcctac cacgtttcta agaaagataa
       61 atgatatcaa aattttggat ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg
      121 cagtaaaata agcgtttgtg tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta
      181 cacatatccc tacacataat aaatttggag gaaaatatgg acttattaat tagaaatatt
      241 gaagaaaagt atattaataa aattgacaaa cgttgtgaag aacttacggt caagactggc
      301 aaaaaatgga gtcgaaatca atacctaaaa gttttgatcg aaaatgattt tgatcacgct
      361 cttctgaatt ataaaaaaga ccaatttgat cggttgttag aaaaatttgt ggatattcaa
      421 acttataata cgaaaacttt agaagaatat attgcaacaa ataatcaatt aattggtttg
      481 ttaattgaat aataaaaata gtattgtgaa tttataaaag gaggaaatca attttggcaa
      541 aagcaacaag aaaacatgta tatctttacg atatgcatat cgaagtactt gatgaaataa
      601 tttctactca tgaagatata tctaattata gtgaagcagt cagacatttg tgtatgatgt
      661 atgatccaaa aatcgaagaa aaaataaatc caattcaaat taaattgaat gcaatgggaa
      721 aagaaatttc tattttaacc gaaatggttt cagagtttgc ggatcttcat ttgaaagata
      781 caggaatact aattggtaca aattcaactg tttatcaaga agcagaagaa cgtgtggaag
      841 gaaaaattaa atccaatcaa accaaaaaat attcttctaa gttaaagaaa ccagtgaatg
      901 aatctgttca taccagagta gatccatttg atccaacgaa gattctaaaa agataaaagt
      961 agaaggagaa cgtgatgaat aaatcgcctg cagttattct tacttgtcaa tttaccttgc
     1021 ccaataaaaa atctttttca acatacattg attatatgac aagagaaaaa gcactggaag
     1081 aaatggaacg acgtacgccc gaagaagaac aggaactgca acgaatcaag aatgctttag
     1141 aggattttta tattcctgaa ggagaaactt tttcggaaag taagacggat aaagaattca
     1201 gtgaaacgtc gaatgaagct caatcgttga tggaacatgg aatcaatttt gacaagttag
     1261 aggaacaaga ttttaccaaa tatctttctt atatgacccg tcactatgcg ttagaacaaa
     1321 agaaagaact gacgcaaagg gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa
     1381 aatatcaaac agagccacat aaaaaagctt caggaaatct tcctggagtt ttttctatgg
     1441 cttcagacga agtgaaagga agtgatctga aggagatcaa acaaatcttc caaagaggac
     1501 aacaaaaagg ctcaatcatt tatcaagatg ttatctctca cgacaattcg tatcttgaaa
     1561 aaatgggctt gtacgatccc aaaacagata ccttagatga agaaggtttg aaagcagctg
     1621 gaagaaaaat gatggagaca ttattcgaga aagaacaatt gaatgaaaca ggttactgga
     1681 tggcaacgat tcaccgtaac actaagcata tccatattca ttttgcagtt gttgaaaaag
     1741 aaaatacgcg taaaccgtat actgagattg aaaatggtgt gagttatatc gtaccgaagg
     1801 ggaagagaag acaagcaaca ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac
     1861 gtttctctta tgaaaaggat cgaatgatcg gtatggagaa acgaaattta ttgacacgaa
     1921 aaagtgattt gcgtaacacc ttaacaaaag tagtaaaaga accagcacgt tacgatcgac
     1981 acgccatgaa cttattaaat gaagtatata aaaccctacc tgaaaaacgt tcggaatgga
     2041 attatggaga tgaaaatcga accaagcttt caccatttac aaggggaaaa cttgatgagt
     2101 taaccaaata tgttttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     2161 cattgaaaga agaagccaaa agcctttatg gggaaagcaa aagagaaaca aagaatgcag
     2221 aaaaaaacgc cctctttgat ttaaaaaagc gcacaggaaa tgcgatttta acagaactga
     2281 agagacagga tagtcaaata aaaccattaa tagaaagtgt caatcagatg caatctattt
     2341 acaatgaacg atcctttcga acactcaaac agttgccaac aaatgaatac aagtatagtt
     2401 atcaagaatt cgtaaagcgt aggaaacaat tccaacaaag agttgtcacg cagcgaagct
     2461 tgcgtcaatt aaatcgaaca attaatgcac atgaagaaaa ataccacgca agtaaagcat
     2521 atgaagagca acagcgaaga atccaaatgg aacaagaaag acaataggag gaagagaaat
     2581 gaaacaatat ttagatcaat ggaaagtcat agaaggatca ctaaaagaag aacgaatcga
     2641 acaacttcct gattgtttag aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa
     2701 cgaacagttt gatccaaatc aatttttagt tgttgagtac ccagctactg gtgtgtattg
     2761 ttgtaatcat gtgaagggtg aaaaatattt tattattcaa gaatacgaag gtaagttagc
     2821 gccttattat actacttggg aaataaatga agagggaatc aataatttcc catgtgaaag
     2881 cattgaagag tctatttctc tcacggaatg ttaaataaat agagacgtaa aagagtcctg
     2941 aacgtgattg tccaggactc tttttcaatg aattgttttc cgccaattca ctgagtggtg
     3001 cgattcaatt ttattatatc aaaagaaagt gagattccaa agtgaaggga ggaagaagaa
     3061 atgactcaga caacgattgg tgttgtggtt gaaaaaagca gggataatct cgtttttgtg
     3121 acagaaattc aaacaggtag agcttttgta gtcacagaca aagctgcaaa agcgtatcaa
     3181 aatggcaata ttttaacgtt aaatctgact acgaagatgt tcgttgatgc agtagaaaat
     3241 tatccatttg tttagacaaa cgatatttta caaggaggaa acggacatgg cagaagaaaa
     3301 agaaccacta gataatagtc ggttgggaaa gtccaaaaga aaattagtac ggttgcaaaa
     3361 cgaattaaat aatcaatttc aaaagctatt tgatcaccaa aaattgacaa atgggcaacc
     3421 aatgaatgac aaacgaaatg gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa
     3481 aatacaaagt ttgagagaag agatcaagca acaagaaaaa caggtagaaa aacttgaaag
     3541 acaagaagag ctaaaggaaa tgggctataa caagtacggc ggactggata tgacgatcga
     3601 taatattcca agaatcaaag aagaaatcga acgctttgaa aaaggtgaat cgactttttc
     3661 agccgccacg atccgaaaat atcaacggaa gttggaaaca ttagaacagc tgaaagaaaa
     3721 atcagaaaaa ggaaaagaaa atatcctccc tgaagtacaa gcgatcattg acagcggacg
     3781 agtgacgcaa tggaaaaaaa atccaaccat ttattttttg aaaggctatc gaaaagttgc
     3841 tttagaactg aatgaaaacg ggggatttga ggaatcaaaa aaataccaag ccacgtcgga
     3901 agaggaaaaa gcagctgttc gtgaattact tgaagaggct gcaccaactg attctctgaa
     3961 ggagaaagaa aaagatcatt tctatcgagt agaattcaac gaaaatgatc cagaaaaagg
     4021 attgaaaagt tatggtgggg agatcgtaac attagaattg atcgatcaac ttaaaacgtt
     4081 ggataaccaa tacgctgcag aagcaggtta ttataaattc tattttgaag aagtgaagaa
     4141 cggagaagta gtagatcaga tccgaatgga tctaggggat ggcgtcaaca gtaatcaccc
     4201 catctattta gagttagcaa aattgtgcga acaagtacct gagaaggaac aaaaacaatc
     4261 ggtcaaaaat gaacctgttt cgaaagaaca aatgcagtcg aaagaaacaa aaacaaaaca
     4321 aaagggctat caaaaacgaa cggcagaaga gatcaaacaa gaagttaaat ccctttcgca
     4381 acaggcactg gatgcggtga aaaagcatac ccattct
//
LOCUS       DACBZN010000155         4402 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.158, whole genome shotgun sequence.
ACCESSION   DACBZN010000155 DACBZN010000000
VERSION     DACBZN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.158"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(99..1619)
                     /locus_tag="IVD64_002706"
     CDS             complement(99..1619)
                     /locus_tag="IVD64_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6149911.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1862..2047)
                     /locus_tag="IVD64_002707"
     CDS             complement(1862..2047)
                     /locus_tag="IVD64_002707"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP6149912.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="IVD64_002708"
     CDS             complement(2031..2213)
                     /locus_tag="IVD64_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149913.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="IVD64_002709"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="IVD64_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3167..3766
                     /locus_tag="IVD64_002710"
     CDS             3167..3766
                     /locus_tag="IVD64_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6149914.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3830..>4402
                     /locus_tag="IVD64_002711"
     CDS             3830..>4402
                     /locus_tag="IVD64_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149915.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         1403 a          799 c          785 g         1415 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 gacacaaaat tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt
     2761 tcttataaat tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca
     2821 tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat
     2881 tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt
     2941 ttttaaaggg gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat
     3001 ccaatgtgac acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc
     3061 taggcagaat tgaatttttg cttggggctt tttccttata ttgattttga atgagactta
     3121 ttatatgtta taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga
     3181 acctattcga gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata
     3241 tggccaacga aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga
     3301 tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa
     3361 agatcgatta gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa
     3421 caaattgcga aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca
     3481 gcaagatttt gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg
     3541 ccaaagtctg tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg
     3601 cccacattcc atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga
     3661 aatcattcag tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat
     3721 tacacaagaa gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt
     3781 ttaaacatgg atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt
     3841 atatattata ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt
     3901 ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa
     3961 tgaaaataat gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag
     4021 tagtttgcat gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga
     4081 ttatctcata ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa
     4141 tatggtttat gattatgttg tttctctaga agaattacaa caacttttca aggaatggat
     4201 acgcaaagaa atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga
     4261 tgtactattc tttacattca attatacaga tgttttggaa attctataca agcaaccgaa
     4321 agaaaacata tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa
     4381 ttttgacaac tttataggag at
//
LOCUS       DACBZN010000156         4332 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.159, whole genome shotgun sequence.
ACCESSION   DACBZN010000156 DACBZN010000000
VERSION     DACBZN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.159"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            127..579
                     /locus_tag="IVD64_002712"
     CDS             127..579
                     /locus_tag="IVD64_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149916.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IVD64_002713"
     CDS             593..1282
                     /locus_tag="IVD64_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149917.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IVD64_002714"
     CDS             1407..3758
                     /locus_tag="IVD64_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP6149918.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IVD64_002715"
     CDS             3838..4233
                     /locus_tag="IVD64_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149919.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1525 a          548 c          867 g         1392 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt tt
//
LOCUS       DACBZN010000157         4295 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.160, whole genome shotgun sequence.
ACCESSION   DACBZN010000157 DACBZN010000000
VERSION     DACBZN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.160"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..792
                     /locus_tag="IVD64_002716"
     CDS             <1..792
                     /locus_tag="IVD64_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6149920.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="IVD64_002717"
     CDS             889..3138
                     /locus_tag="IVD64_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP6149921.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="IVD64_002718"
     CDS             3141..>4295
                     /locus_tag="IVD64_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6149922.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DACBZN010000158         4287 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.161, whole genome shotgun sequence.
ACCESSION   DACBZN010000158 DACBZN010000000
VERSION     DACBZN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.161"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(127..1293)
                     /locus_tag="IVD64_002719"
     CDS             complement(127..1293)
                     /locus_tag="IVD64_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP6149923.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFALSMILLLSFLWLL
                     VRKCELTNGQLTFLWAFLAIMCQSFMEETLATISWVPIVFLMLAMATHQYKTTR"
     gene            complement(1299..1988)
                     /locus_tag="IVD64_002720"
     CDS             complement(1299..1988)
                     /locus_tag="IVD64_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP6149924.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDDVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(2115..2354)
                     /locus_tag="IVD64_002721"
     CDS             complement(2115..2354)
                     /locus_tag="IVD64_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149925.1"
                     /translation="MNCKESVNWKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKTSSNCQLLLEVS"
     gene            complement(2421..2660)
                     /locus_tag="IVD64_002722"
     CDS             complement(2421..2660)
                     /locus_tag="IVD64_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149926.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(2774..3115)
                     /locus_tag="IVD64_002723"
     CDS             complement(2774..3115)
                     /locus_tag="IVD64_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6149927.1"
                     /translation="MKNYDPNIRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEEDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(3735..4067)
                     /locus_tag="IVD64_002724"
     CDS             complement(3735..4067)
                     /locus_tag="IVD64_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6149928.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYVGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT         1334 a          871 c          594 g         1488 t
ORIGIN      
        1 gacgattaaa gcgactccta aaaaaccaaa taaccatttt ttcatccatc tgcctccttt
       61 cagacaaaac aaaaaacact ttttcacaac gaaaaagtgt tttctcttat tactattcta
      121 tttcatttat ctggtggttt tatattggtg tgtagccatt gctaacatta agaacacaat
      181 tggtacccaa ctgattgtag ctaatgtttc ttccatgaaa gattgacaca tgatcgctaa
      241 aaaagcccac aagaatgtta gttgtccatt tgtcagctca cattttctta cgagcaacca
      301 taagaaacta agcaaaagaa tcatcgatag agcaaaacca atcaatccat agtgattgac
      361 gagtgcgtga tacgtattat ggttacctag aatctgattt cctctatgga aaaagaaagg
      421 tttcatccct acaaatattt gtcctgtttt ttcgcctaat gtttcataga atttgcgcca
      481 aatttcttct cgtcctgtaa ataaatgaac tttgtctgac attgcagcaa aataagaaat
      541 cggtaaagct agaaccgaaa ctgctgcata catccaaaag gacaagtgtg tgtttcgaaa
      601 gatcttctta ggtaaaattt ctaggataac gaagataaca agcgcgtata atgctgcttt
      661 cgattggcaa acccaaatcg ttgccccagc aagaatcaac gctggtaatg atagcaatcg
      721 catgtaccat tttccaaatg aactagcaaa tcctgtgata agaagggcta atgtcattaa
      781 agaactacct atcgtatttg tattgatcca aatatctgat aatttgttcg atggattcca
      841 aatactttgt ccttctggta ctggtatctc ggtaaatact cgataaaccg ttacgagtaa
      901 aaagacatag aatactacaa gatacaagct acgatcaaat acagtaaatt ccgtccattt
      961 tacaatcaaa ataatggcaa taattgtcgg aacgatcaga tcaccataga aagtatctcc
     1021 tcggtagctt ctaaaaaaag aaaagaaaag gtaaaaaaga atgcctgctg caatgaagaa
     1081 tagatcgttt ctctttacct tgtacagatt caaaagaaac aaacagccta caaaaaacgt
     1141 cactaactgt gcagcattat cataaataaa taatatcgta ggaggattta accccaccgt
     1201 ccatattaaa taaagaatgg ataccaacag taacagaaaa atggcactca ttttattgat
     1261 caatttttct acaagttctt gaatgtgact cacttccctt atttattgat tttttctaca
     1321 ttgactaatt cgcactggat cacatcgcac tttttcatat tatttgcttc aaagtctaac
     1381 agtgtgagta gtggtttttt acctgctttt ggcaaggata aaaaggaatt gttttcctta
     1441 gattgaacta cctggctgta atcataaatt tttcctcttt tggagacttc atactgatac
     1501 ttgtaatgag catcgattag atcaatttct tctccttcgg ataaatcaat catatcggat
     1561 aataaaacgc ttggtgtatc tacgttataa ccagcaagaa tataatttcc atttcccatt
     1621 tcagcatctg gataataagt agaagctact cctaaactaa gcgtgtcgct attcgctcct
     1681 tgaaaaacag ggagaataaa tcctgtagaa gaaaaaacaa gttttccagc aatttcacct
     1741 ttgagatcct tttctaatgc ctgtttttga ttagtaatca gttgtctctt agaaatatcc
     1801 gttaaactgg ttaaattatc ttgataggtt ttagcctgtt cctgaatatg gattgttttt
     1861 gttacatcat ctactttttc agcgattttg tccttttgat aaccttgata agcaaaataa
     1921 ctaaaaagga gaaaaacaat gattacgccc acgtaaggat taaataaagt tttattgttt
     1981 ttattcatct atctaccttc tatatattaa ttaatttacc tacaactaac caagataaac
     2041 cacgttaaat tttactattc tttttattca ataacaatgc cacatcataa ttgatcattt
     2101 tcatatcata atcgtcaaga aacttctaat aatagttggc aattagaact tgttttcaca
     2161 taagttcgtc tcaactgttc aaaatttttc attgatccca atagacaggc tttctccttt
     2221 cctgttatct gcatatcatt gatatattgc atgacagata atcttttaag gtgtttttgt
     2281 ttcatcattt ttagcaactt ttcttccatt tcttttgaca cttttttcca atttacgctt
     2341 tctttacaat tcatgagttt tcctaccttc cctgtttttt gcaaaaataa aaaagcctat
     2401 gatcacatac gctctacact tcagtcttgg gttatctttt tctttcgttt aattactggc
     2461 cgtgctggtt tcaaatcctt gattccatta tgggttctcc aataatcgaa ttcttttcgt
     2521 aatctttctt tttccatatc tgttccttta gatagggctt tgaatacatc tttaggtgaa
     2581 taaccacgag ctaacagctg tacatacctt ccttttgtca aagtatccat atccatcgcc
     2641 tctaaaactg gtcgcgccat ctaattttcc tcctaaataa attccttact caatgcctaa
     2701 ggtaatacta cctttttatt aatcgtcgtg actccttcta agcctttatc tgtaaaattt
     2761 attcctccag tttttattct tctttatccg ctacaaattc aatgatttca acgctgacaa
     2821 tataatcact caaatatgac caagtatctt caacagataa ttcgtctcca ttatcattca
     2881 taagcgtcat tttaaaccat tcattacctt catcatcttc aggtagcaag ccgaatccaa
     2941 ttggattatc agtcaatttt tgatcgtata aatcttcctc gtctaatgct aaaacatcta
     3001 atcctttaca atttccgcct cttctaaacg tcacaaatcc tttgtaatcc caacgttgga
     3061 aagaaacctt aatagtatgt gtgccccatc gtatattcgg atcataattt ttcatttgtc
     3121 attcctccat tacgtaaatt tcattttctc tatgttttat atcaattttt tcactttatt
     3181 caggactcgt tcatatttta tcgtttttcg tttacttaaa aatacataaa aaatatcgac
     3241 tcgtttatgg cgtccgaaac gagaaaccct agtagaacat cggtaaatgc tcgattgatc
     3301 caataatcaa cgtcctcgca agtatctaca tgtactgcag cacacgacac aatttcttgt
     3361 gcgtcctccc cattacgaga agacctactc cttattccat gagtttgcgt caaagcctag
     3421 atcccttagt agctgctcat gattaatcta ggcgctattc taacttgtct aacttctaaa
     3481 ttccctcttg tttactctgt gcatattatt tacacaaatt catttcacca ctatccaaac
     3541 aagggcattt actcgattga ccaatttttt acgttaccaa tcgtaaaaac ctaccccatc
     3601 actccccgaa gggaattgtt gatagatcaa gtctactcct tatttttcta gtgattctaa
     3661 gttcttttag aagtctcgac aatattttca agcaagcgaa aaacgataaa atacaagtta
     3721 taagaatgat caattcagct gatcaaacgc cccatccctt ttgcggtaca acgtaagact
     3781 ccattttttc ttcgacctat caatacaccg ttttggtcat aaacatgttt gattgttgga
     3841 tatagctctt ttaaagggat aggatctttt aaaattattt gcttttgttt cccaacgtaa
     3901 taaatcattt cctctgagac tttgtctttt tggattatat atttcttaca ctgcacaacg
     3961 gttttatttt ggccagttga tagtttcttc gtgatcttct ctgtagcggt acaccgttta
     4021 ccgtagtgac aaatatcttc gagactcaaa attcctgtat atgacataaa ataatccacc
     4081 cacttacttt aaatattttt aacaagtttt tcttgttatt tttcaaaaat agagaattga
     4141 ttttcgttca tgacaaacaa aaaagaggga acgcatccaa acatgcgttc cctctaattt
     4201 cagcgttcat catacggcaa agagccacac ggacattttt ataacattat gcttaggcat
     4261 agattcatcg taatatcacg tattttt
//
LOCUS       DACBZN010000159         4282 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.162, whole genome shotgun sequence.
ACCESSION   DACBZN010000159 DACBZN010000000
VERSION     DACBZN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.162"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(37..1257)
                     /locus_tag="IVD64_002725"
     CDS             complement(37..1257)
                     /locus_tag="IVD64_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6149929.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IVD64_002726"
     CDS             complement(1277..1882)
                     /locus_tag="IVD64_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6149930.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IVD64_002727"
     CDS             complement(1931..2863)
                     /locus_tag="IVD64_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6149931.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLEELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IVD64_002728"
     CDS             complement(3284..4144)
                     /locus_tag="IVD64_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6149932.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1220 a          861 c          699 g         1502 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactcttc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc aaacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DACBZN010000160         4270 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.163, whole genome shotgun sequence.
ACCESSION   DACBZN010000160 DACBZN010000000
VERSION     DACBZN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.163"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..252
                     /locus_tag="IVD64_002729"
     CDS             <1..252
                     /locus_tag="IVD64_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6149933.1"
                     /translation="PVDWTDAADKKGEPDTPVLYFGDFSAFKIQEVIGALEIQKLVEK
                     FSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITKPVGG"
     gene            254..532
                     /locus_tag="IVD64_002730"
     CDS             254..532
                     /locus_tag="IVD64_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP6149934.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            513..839
                     /locus_tag="IVD64_002731"
     CDS             513..839
                     /locus_tag="IVD64_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6149935.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            829..1167
                     /locus_tag="IVD64_002732"
     CDS             829..1167
                     /locus_tag="IVD64_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149936.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            1157..1522
                     /locus_tag="IVD64_002733"
     CDS             1157..1522
                     /locus_tag="IVD64_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP6149937.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            1529..2155
                     /locus_tag="IVD64_002734"
     CDS             1529..2155
                     /locus_tag="IVD64_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6149938.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            2155..2619
                     /locus_tag="IVD64_002735"
     CDS             2155..2619
                     /locus_tag="IVD64_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149939.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            2814..>4270
                     /locus_tag="IVD64_002736"
     CDS             2814..>4270
                     /locus_tag="IVD64_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6149940.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGP"
BASE COUNT         1504 a          706 c          915 g         1145 t
ORIGIN      
        1 cctgtggatt ggacagatgc agcagataaa aagggtgaac cagacacgcc agttttatat
       61 tttggcgatt tttctgcatt taaaattcaa gaagttattg gtgccttgga aattcaaaaa
      121 cttgttgaaa aattctctgg taaaaatcaa attggattcc aaatttacaa cttgctagat
      181 ggtcaattgg tttattctcc atttgaaccg gcagtatatc gctacgaaat tacaaaacca
      241 gttggtggtt aagatggaag agcaaactaa agaattgtct ttagaggaaa aattcaaatc
      301 acatattcat tttgaagagg gcatggatga ttctttgctc tctttttatt taaatatggc
      361 aaaagattat gtcaaaacag caactggtgg ccaacaagaa tatcttattt tgatggttgc
      421 tggcatcgcc tacgaatacc gagtatctga agatgagtta gacaaagcta tgaatgccat
      481 gacgccattt atcgtgcaag gagcgattca aaatgccgaa gagacagact aataatctca
      541 gatggaaagc cgaattgcta gacatcaaaa caggaacaga cggaaacgat cgtccaacta
      601 caatttacga atttaagcgt ctaatattct atgaagaact cggtgtgact tctcaagaaa
      661 aatatttatc acagcaagcc aagacagacg ttgtcagacg aattaaagtc agatgggata
      721 aatccatcac agagaaatta agtgcgctca aaattgattc tgtaacgtat aacattactc
      781 gcatttatac gaatcccgat gcaagagaaa tggagttgag tttagcttat gtcgattagc
      841 tttgatgaat tgaaaacagc gctgaaatca acaaagttag cagtgttcag agacaaagcc
      901 agattaggga cgatgtatcc atacattgtg tactcaaatg tgagtaacag caaaaaaatg
      961 gcatccggta aagtctataa aaaattacct tattaccaag tctctttttt tacacttgga
     1021 acagaacagg atttagttgt tattgaacaa gcgctgcaaa atgctggcat tccatattca
     1081 gattttacag gtatacaagg tgatgagaac gacgataccg taaccaacta ttacacatat
     1141 gtgaggtgtg tggaaaatgc caagtaataa gaatggtttt tctgaaatat cggattattt
     1201 aggaaacctt tcaaaagttg atccgaaaaa attatcgttg gaatctttag aagaagcagc
     1261 taacttttat ctaaagcagt tgcttcctaa tattcctaag tcgctcttaa aaaagaaaca
     1321 tatgcgcgat caaataaaag tagtagttga aaacgatcaa gtgaaagttc aatttgaaga
     1381 gacggccttt tactggcgct ttgccgaaaa tggcacaaca aatcaacgtg cccaacattt
     1441 tgcaagtggt acgtatgaac aaaacaaaga aaaaattgaa gaaattatga ctagaaaaat
     1501 acttgattta tgggaagggt gattttaatt gggaaaacaa gatacttttt attttgaagg
     1561 attggacgac attttaattg cgatgatggc aacgccagat tctgttggaa ctgcaccgac
     1621 ttatagtgaa gttttacgat tgccaattgc cacaaaaatc ggtgtcaaag ggaatggaac
     1681 agcattagaa aaatgggcat ccagcaaaat gtttcgtcgt gtgtcgcgag aaacaaaaca
     1741 tgaacttgcg ttggatcacg tgggtattcc gatagaagtg atggatgaaa tcaaaggatt
     1801 agttgctaaa agcggagtaa cgtttagcaa aaataccgca cgagaatttc cgtacttcgc
     1861 ctttggattt attggaaata ttgaaaatgg cggtaaaaaa gcagtttggt atcctaatac
     1921 acaactatcc aatgtgattg acgaagagta tgccactgca gaggaagaaa caaaaattga
     1981 cgatgtaact gcaaatctgg tttccatcgg actgaaatac aataatgtga tgtatgcaag
     2041 ttttgattca aatagagacg gagcttctct agaattattt aataaattca ttgctcaacc
     2101 tgtatacgat gaagaacagt ggaaaacatt ggctaaagtt ggaggtgcgg gttaatggct
     2161 cggttatctg attatggaat tcacgttgaa gacttaaaaa attctgctgt tgtaattatt
     2221 caaggcgcag aattccctat ttcatttact atgcaaacga tggaatttat agcagatgtg
     2281 tatggtggag attattcgca atttgaatct gatatgaacg ccatgctatc taaaaaagaa
     2341 ggacaaattt cttctgctaa cttatcgcct agtgatctaa aaatcatgcg tgccttgatt
     2401 tatgccatgc tgcgtactgg tggtttagag gaagatccag aaactatttt caaatttttg
     2461 ggaatgagtg gagaggtgtt gtctgcttat agtacctgta tggaaatttt tgctagccag
     2521 acatttcagg tggaagacct aaaaaaatcc aagaagccac aagactttca aaaaacgcaa
     2581 gcaaaaagaa aggtaaacaa aaagaatcgg aagagatagg aactccttgg agtttttata
     2641 tttacgttgc tctcactcta ttgaattgga gtgagagttt ctttttgaag tctacaccta
     2701 acttgtggct caaatcttat ttacagtggt tacaacaaaa caccgatttt gaaccacctc
     2761 aatctgtaac tatggataaa tcgccttggt ggtagaaagg agcgctaaga taaatggctg
     2821 gtaaagaatc tgatgtcgtt cttaatttta aaacgaatgg cgaagtcagt tattcgaaaa
     2881 caatcaaaga aatcaacaaa gaaatgaact tagccgctgc cgagtacaaa aaccaagtgt
     2941 ctgcgatgga caaggatgca actcaaacag aaaaattgcg agcggctaaa caaaagttag
     3001 aaaaacaact ggggttagct gaacaacgat ctcaaatgct gagagaggaa tatgaaaaat
     3061 ctgtcaaaga aaccggtgaa tattctgctg aatcagaaaa actttataaa caattactca
     3121 attctgaaac cggagaaaat aaacttcgta cagcattaga acagacgaac gatgcactta
     3181 aagaacaagg tgacgtttct gttgatacag caaaaaaact ccagaaaatc gaagaaacag
     3241 gtgagaaagt aaaaggcgtt ggtgaaaaaa tgtctgttgg agtaaccgcg cctattgtag
     3301 cggcaggagc agcaggactt gcagcatttg gtgaagttga cgaggcactt gataccatca
     3361 ttacaaaaac cggagcaaca ggtgatcaag ctgatagact ttcacagtct ttcaaaaacg
     3421 ttggttcaaa tactcattta cctttacaaa cggttgggga agctattggt gaggtaaata
     3481 cacaatttgg attcatggat aaaaaactgg aagattcaac caattatctc ctacagtacg
     3541 ctgaaatcaa tgatacagat gtttcgcaat cagcaatatc tgctcgacaa gctattgatg
     3601 cttatggact agaatatgat gatttgaatt ctgtccttga tgtaacaacg aaaacatcgc
     3661 agaatactgg tcaatctgta gacgacttga tgcaaaaagc aattgatggc gcaccacaaa
     3721 ttaaacaatt agggttgagc tttggtgaag gggtcacttt acttggacaa tttgagcaat
     3781 ctggtgttga ttcaagcgca gctttaagta gtttatctaa agcaacagtg gcttatgcga
     3841 aagacggaaa atctttaagt caaggactag aagaactaca agacaaggta aaaaatgcag
     3901 gttctgaaac agaagccatc aatgcagcag cagaagtatt cgggactaaa ggcggtccta
     3961 gaatggccga tgcaattcgt agaggtactt tgaacctgga agatttagca aaaactgctg
     4021 gagaaagtgg gggagctgta ggagacacat tcgatgctac tctcgaccca attgatcaag
     4081 ccgatcaagc tatgaacaat gcaaaattag caatggctga cgtaggtgaa gcagtccaag
     4141 taagcctttt gccttttttt gaaaaagcaa cttccttatt acaggaattc tctaaatggt
     4201 gggggtctct agatcaagat acaaagaatt ggatcataac aattgctggt atagcggcag
     4261 cagttggacc
//
LOCUS       DACBZN010000161         4259 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.164, whole genome shotgun sequence.
ACCESSION   DACBZN010000161 DACBZN010000000
VERSION     DACBZN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.164"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(98..1270)
                     /locus_tag="IVD64_002737"
     CDS             complement(98..1270)
                     /locus_tag="IVD64_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP6149941.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1377..2696)
                     /locus_tag="IVD64_002738"
     CDS             complement(1377..2696)
                     /locus_tag="IVD64_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6149942.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(2693..3346)
                     /locus_tag="IVD64_002739"
     CDS             complement(2693..3346)
                     /locus_tag="IVD64_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149943.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            3634..>4259
                     /locus_tag="IVD64_002740"
     CDS             3634..>4259
                     /locus_tag="IVD64_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6149944.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPR"
BASE COUNT         1316 a          715 c          565 g         1663 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaagt caagtccaga
       61 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
      121 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
      181 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
      241 tcagtcgttc aattagattg gtactcttta gtcgattgtg ggaatttcct tgtacggtat
      301 attgaaaggc gtcttcgaat ccatcatcca atgatgcgca agcttttgaa tattttggtt
      361 gatcgatata atcatgaatc aatcgatttt tagcctcacg cgctaagtta atatctgtga
      421 acttaaaaat tcctttaaca gcttctctga aagattttga atttttttta ggaatggtgg
      481 taaagatatt tcttaggaag tgaacttggc atctttgcca acttacgttg gtgaaggatt
      541 ttctaatggc agagactaat cctttgtgcg catcagaaat aacgagttcc gtaccttgta
      601 aaccgcgttc ttttaggtat tcaaaaaatg ttgtccaggt ctcttcgctt tcgccacttt
      661 gaatcatgaa gccgataatt tcacggtcgc catctttggt tattccaatc gctatatgac
      721 agctttttga gagtactcga ttttcttctc gtacttttat atagagtaca tcggtcatta
      781 agtaaggata atttttttct gataataaac gattctgcca ctcgttaacc ataggttcta
      841 gctgttctgt taagctagaa acgaaggact tagagacgga tttaccacaa agttcttcca
      901 caatttttga tactttacga gttgaaacgc ctgatacata catttccaac attgaagcca
      961 tgagggcttt ttcgtttcgt tgataacgtt caaacactgt gggtgaaaaa tggccatcac
     1021 gtgttctggg tacttttaat tctagcgtgc ctacacgtgt cgtaaagctg cgctcataat
     1081 agccatttcg ttgactttgt cggttttctg ttcgttcata ttcttttgct tgaatatatt
     1141 ctgttcgttg attttccatt agttgattaa ataccgttgt taaaatattt ttagaaacgt
     1201 catcctttac agaatattca ataatgcttt gaatctcttc gcttttcagt gtaaaatgta
     1261 cttgggtcat gtaaaagtcc tcctgggtat gtttttgtcg ttaaaaacat tgtaccgtaa
     1321 aaggactgtt atatggcctt tttactttta cacaattata cggactttat ccttaattaa
     1381 gaaaatattt ttattttaaa gataaaaaat tcttcatcct gcaatacttt atattctata
     1441 ttgtaattat tcagaatatt acttacaata tataaaccta atccattact attttcctta
     1501 tttaaatcaa atttaacatc aaatatttta tccatattcg aaattttatt gttaccgtat
     1561 gaattctcta tatataacca atcattaact atcccaatat taattacccc atttacatca
     1621 gtatatttta ccgcattact aatcaaatta gatagaataa tctttaaagc tgtttttcct
     1681 atataaacat tctcgtctaa tatataatta ttgacagtta ttttcttttg atttgctaat
     1741 atttcatact tttttaatat gtcttctaat gtatcattta ttttcaaata ttcttcatca
     1801 ttattcaaat tttctataga atgaactgat aatatttgag aaatattttt tgttaaacta
     1861 tcaacgattt caatacattc attaatataa atatctcttt ctttatattt tccaatattg
     1921 tatttcatgt tttccagtat tattttaaga ctagcaagag gggttttaag ttcatgggat
     1981 gcacctttaa aaaagtcata ttttaacttt tctaatttta aaatttcttt atttttaaat
     2041 tccaaatcgt caattgttct taataaagta gaatacaaat cattaatttg ttgttttaat
     2101 tctccaactt catcattaga gctaactttt aaactcattt ttttatcaag tttcatcatt
     2161 ttatcagtaa caatttttat ttcttgtata ttatttttta ttgattttgc ataaattaaa
     2221 gaaataatag cagaaaataa aattgatatt aataaagaat atggtaaaaa tttaaggctt
     2281 aaatctttcg catctttttg catatcagct gtagaaacaa attgtagatt tgttttttta
     2341 ccatcattaa gctttatttc tctttcttca attatcaaag aattactatc actttctaaa
     2401 ttaacattta tattatcttt gatttgtatt tcattcttat tatttttttc ttttatgaat
     2461 acttttattt cactactctt ggaataaaac tccaaagttt gttctatata ttttatttct
     2521 tttccattca tattactaga aatttcattt gccatattat gaatcttttc ttttctcgtc
     2581 tccaaatatg ttttaggaaa gataaaaaaa attaatgaat gaactaatat aattattatt
     2641 ccaagaacag aaaatatttg tatgaacatt tttggaaata tctttaattt tttcattttc
     2701 tctccaattt atatcctaca tttcttatag tagttataca gtctaattgt aatttttttc
     2761 taagttcttt tatatataca tctattactc gatcataagg agtttcttcg ctatctttcc
     2821 atacataatc aataatttgc attcttgtta acacttgtcc attattatcc aataaacatt
     2881 ttaatacttc tagttctttt gcatttacat ctatttcttc accatttatt ttagctgtat
     2941 agctattaaa attaaccgat agatccttat attcaaactt ctctaaatgt ccataattct
     3001 tcttaattaa agaatctatt ctagctttta agaccggcaa tgaaaatggc ttttctacat
     3061 atccgtctac taaattagta aatgcatcaa ttttatattc ttcatcacta aatgcagtca
     3121 agattagaat tggtaaattg cttttctttc taatttcttt caacacttct aaaccattta
     3181 taaaaggtat ctgaatgtct aagataacca aatttatatc gctattaaat tttgacaagg
     3241 cttctcttcc atctttagct tgaataacag tatatccaaa ttctgaaaga tattcactta
     3301 ttccctctct tatcatatta tcatcttcaa cagttaatat tttcatatat acctccactt
     3361 aagcctatac actagatatt atacagcata ttatggctct ataatatcta gtgttggtga
     3421 cgccttctgt tgccaatact ttttcttttt tttaataaat gcaaacagac accttgaaat
     3481 cctttagaat aaagggttct ataatatttt gtgttgttgg aaaatcgaag gcgattttct
     3541 actaacactt tttcttcgtt tcaactcaaa aagagactca cttttggtaa aatttagtcg
     3601 accaaaacat caccctacca aaggagtcgc tctatggata atcatactag aaaattactc
     3661 ggattaacag ataaacattt attttttgat gaagaatggc tgattgaaaa agaatataaa
     3721 ggcgttcaag cgcagtttat taaagggaga ttggatgact caccttccca ttgtgaacac
     3781 tgtggatcga ttcgtattat ccgcaacggt tcctatacga cacgtactca gatattgaaa
     3841 gtcaaagaaa agttgactat tttagaatta aaacgtaccc gttttctatg ctatgattgt
     3901 gggcagacct tttctgcgaa aacggactta gttgatgaac atcatcaact aacaaaggaa
     3961 ctgaagcagg cgattctcat agaactgtac gaaaatcaat cacgtaaact aatcgctaag
     4021 aagtacttcg tctcggatgg gacggtgaca cggatcttgc gtgaagcaac gaagcattac
     4081 cagccacgta tgaacttcct tccgacggtg ttgtgtatgg atgagttcaa atcaatgaag
     4141 tctgtttctg gttccatgag ttttatttgt gttgatggga caacccatca gctatttacg
     4201 atccttgaag atcgcagact ttacaaacta actcaatact tcctgcactt tcctagaaa
//
LOCUS       DACBZN010000162         4213 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.165, whole genome shotgun sequence.
ACCESSION   DACBZN010000162 DACBZN010000000
VERSION     DACBZN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.165"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(113..202)
                     /locus_tag="IVD64_002741"
                     /pseudo
     CDS             complement(113..202)
                     /locus_tag="IVD64_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(223..468)
                     /locus_tag="IVD64_002742"
     CDS             complement(223..468)
                     /locus_tag="IVD64_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149945.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(428..784)
                     /locus_tag="IVD64_002743"
     CDS             complement(428..784)
                     /locus_tag="IVD64_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP6149946.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(784..1089)
                     /locus_tag="IVD64_002744"
     CDS             complement(784..1089)
                     /locus_tag="IVD64_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149947.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(1086..1247)
                     /locus_tag="IVD64_002745"
     CDS             complement(1086..1247)
                     /locus_tag="IVD64_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6149948.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(1262..1621)
                     /locus_tag="IVD64_002746"
     CDS             complement(1262..1621)
                     /locus_tag="IVD64_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP6149949.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(1618..2469)
                     /locus_tag="IVD64_002747"
     CDS             complement(1618..2469)
                     /locus_tag="IVD64_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6149950.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(2486..3316)
                     /locus_tag="IVD64_002748"
     CDS             complement(2486..3316)
                     /locus_tag="IVD64_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP6149951.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(3319..4005)
                     /locus_tag="IVD64_002749"
     CDS             complement(3319..4005)
                     /locus_tag="IVD64_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149952.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(4011..>4213)
                     /locus_tag="IVD64_002750"
     CDS             complement(4011..>4213)
                     /locus_tag="IVD64_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149953.1"
                     /translation="DPDWGKSNLQLVANKYEKSIDEAIVALTDAASITADDSKWTKRD
                     LGILKRGVNWLEDQYREETKEK"
BASE COUNT         1018 a          932 c          642 g         1621 t
ORIGIN      
        1 cttggtgtct catatttctt tacatcgaaa agtaccatcc tctgattccc ccaattatga
       61 tataatactt atgtcggaaa tattattcat agtcggagga atccgacttt ttttattttg
      121 tacgtgaaat gagttctcct gatttatacg cctcagcaaa ttcaaccaaa gctactgcct
      181 tcattctgtc tttttctcta tcccacgctc taaacttcgg tatcattcgc tgtcctcctc
      241 gtatttttca atcaattcca ttactttttt cactatttca aactcaaccg cttttgattc
      301 ttcgaaatca tgaacaattt ctggaaacag tacatcatca actacccaca aaataatctg
      361 gcgctttcca ccaaaattta tgattagatg gtccgattcc acagatagtg ttgctcctga
      421 ttcgatatca tataaatcca tgctgaattg aacgagtttt tttatcattt gctgtcctcc
      481 tcaaatactc ttctaatatt tctttatact tctctacaaa tttgaaacga tcttgatgaa
      541 gtttcttgct ccaatttgtt tgccgatcca gctcacgcat ctgatcgaac cctttttgaa
      601 tttcgttgta ataaaattca atgtttgctg ctgctttcca atgcctgcta cttcgcactc
      661 ctgctcctgt ttcagccatt tctaacttaa ctaattcagc tcgttctttt gattttttgt
      721 ctttctgaat cttcatcatg attttcttga ggatgatgtc actgtattgt gtaatgagat
      781 ccattatctc tcctccacat acctaaactg tcgtcctttt gaatcaatcc ataagctcct
      841 agctctatcc cagataatgt ttttgcttaa tccagtaatt tcagataact gttcagctgt
      901 acctgttact agaattcgat caccatgcca gattgcaatt tttctcggcg ttttccgttt
      961 aggcttttca gtccacattg atttaccgag cttttggact tctgcaacta tttctttgtc
     1021 ttcctgccaa gattctgact tggttaattc agcaattcgt ttcattgtcg ctttcttatc
     1081 cacgctcatt cctccaatct acgaatttcc cttcttaagt tctctatgtg caaatcgatt
     1141 gcctttctcg ccgtttcatt gaccatcact gcctttgttc gttctagatc gtcaatttca
     1201 cgctgaatgt ttcgaatacg catttgaatc acttcttctg ttgtcatgat ggaccacctc
     1261 gttaaaaacg ctcttccttg aacgtattcc gatatttttt agctaatatc aacggcgctt
     1321 gatattgatg acagaacaac tttgccttga tcttaaagtc ttttgtctgc attcctttga
     1381 catctacgac tttgacaagt ttgccgtttt tataaaatgt gaagtcggga atatactcga
     1441 tcttgcgata cttctttccg tctagttcaa atttcggcat cagctcaaat ctttcctgaa
     1501 gttttacttt ccagccattt gcttcagctt gccacaaggc tagatcgtaa tactctgctt
     1561 ctgcgataga atcaaacttg atacctcgat gaacagtttt tttattacgg tatttattca
     1621 tgcgatacta cctttcactg gttttatgcg cttgtctgct gtttgttgga atttcagcgc
     1681 ataaccttct gaattcttaa atatcctaga aacaattctt tcaccgtagg cttctcttag
     1741 ttcaggacca gataagtttg ttgtgatgat cgttgccttg ttctgtctgg cttctaagag
     1801 cgtgtttaac gtgttgtttg taaactgcct actatttgat accccactac ctaattcagc
     1861 tccaatatcg tcaaaaacca ccaaatcagt tgttttaata tcggctataa gcgatccttc
     1921 aatttctttt ctcagttcag tattgttata agaaaacttt atttgctcta ataattcttg
     1981 atagcttata aaaagtattt tcttgtcata atttgagcgc tcaagtattt cccaagctgt
     2041 ggccattgat aagtggcttt ttccgcttcc tgatttccct gatagaatga aatgtgcagg
     2101 atggttcagc agaacctcat tcgtatagct ttttgctttt tctaaagcga tttttgtttc
     2161 ttgatccact acgtgataat tttccatttt gcatttaaac aaagttttat ctgttaatac
     2221 cgaaccattt tgaaaaaaac tcaacgctcg tgcttttaag ctatcgttat atatccgttc
     2281 ggtctgtata tcctctttca cacgtaacgc tttataacca caactcatgc atgttggttt
     2341 acaacgttct gaaccatcct tatttttagc tcgccaacta tacaaaggtt cgctacattc
     2401 tggacatttt ccgctttgca ctaatactct tcttattagt ttctccatag catttgctag
     2461 gctttccatg tgatgcatct cctttttaaa ttggcaagtc gtcatattca ctaggattgc
     2521 tgtactgtag tttttgactt tgctttttat gattcttctt gtctgctttg atttcgaatt
     2581 tgagcttctc aaatttttct ctcaatttct tagcacttct aatatttcca aaccaaaatt
     2641 catttgtagg tagccaattg atcacatact caatcgcttc tatagatgct ttatctcttt
     2701 cttccatcaa cctgattgtg tctgcccatt tttcgatatc tactttgttc atttcttttg
     2761 gaaaatcttc agttaaatta ctttgcaact ttttagcaag gcgtaagtgt tcgtcagaat
     2821 acttaccttt cttttcttct ttatctatat ctttatcttc ttctatatct ttatctgtac
     2881 cgtcacgtga cgtcacgcta acgtcatttt ccaatttgag acgttcctgt ctctttcttt
     2941 ccctgtattt acggtttctt tcagcatttt ttagccttac tttatccata ccctcgatat
     3001 tttgatgttt ttcccaatta ctgatggcaa ttagtccatc actgcttaga tcaatcatgt
     3061 tgaaatttgc caatgtagtt agcgctaagc gaaccgtatt tacgtttttg ccaaacaatg
     3121 tagcaagcat ttcttcggta taaggcatgt tcctctggat atatatcaga ccatcgtcgt
     3181 tagtctttcc tgctaaaact agtaatcgaa tccatataac gatgatggca tccgactcag
     3241 gaacagcttg gattaatcgt attttttcat cgtcaaacat agtagtttta agtttgatcc
     3301 aacttatctc agccaaattt atcctcctat ccttaacttt ttaattgttt cctggtttaa
     3361 cttgatccct ttgatttgat atttattttt gaaattgatc acacctattt tgtgtttctc
     3421 tgtgtgatgg attctgcaga gtgctgcaaa tgtgtactct gaatgatcaa cttctttgcg
     3481 ctttcgtctt cccagcgctt tgtcaaagtg atcgatatca gctcctgttt tgccacagat
     3541 gcagcagact ctttttgtaa tgcatttgta gaagtaatac tcttgattcg ctggtaaaat
     3601 ctcatatcct tctttgaaag gaatatgatg ttcaaagatg aaatctagga tgatattcgc
     3661 taagatattg gcatcactca cggttgtatt cgattcgtct ttgaggctta tttcgcgccc
     3721 tgtgacacct tcaaaacgga agtagaagaa ttccttccag aagtccgttg gcatgcctgt
     3781 atcgatgaaa atatcgccta tgagcgcata gatgaagttt ctttgctgta ctgtgaaccg
     3841 tcgtggatca ataaagcgaa tttcaatgac tcgatcgcca tcatatccgt catacatcgt
     3901 tttcagccgt tcgatattta cttcttcgtt gattgttgca ccaatgtcgt tacctttaaa
     3961 tttctttagt accgccgagt atgaatctat tagtggttta aacactcata tcacttctct
     4021 tttgtttctt ctctgtactg atcttcaagc caattaacgc ctcgttttag aatgcccaag
     4081 tctctcttgg tccatttact gtcatcagcg gttatggaag ctgcatcagt caatgcaaca
     4141 attgcttcat caattgattt ttcgtacttg ttagcaacca gttgtaaatt cgatttgccc
     4201 caatcaggat cgg
//
LOCUS       DACBZN010000163         4156 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.166, whole genome shotgun sequence.
ACCESSION   DACBZN010000163 DACBZN010000000
VERSION     DACBZN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.166"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            674..1363
                     /locus_tag="IVD64_002751"
     CDS             674..1363
                     /locus_tag="IVD64_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6149954.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1350..2558
                     /locus_tag="IVD64_002752"
     CDS             1350..2558
                     /locus_tag="IVD64_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6149955.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2852..4123
                     /gene="serS"
                     /locus_tag="IVD64_002753"
     CDS             2852..4123
                     /gene="serS"
                     /locus_tag="IVD64_002753"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP6149956.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1338 a          719 c          826 g         1273 t
ORIGIN      
        1 gtttgttgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctacttca tcaggagtgt ttcccgattc gtttttggct gccgtagcaa
      121 cttttatatc atatcagaga gacaagtaat tgtcaactct tttttaaaag ttttttcgat
      181 ttcgtttttc gaaatcttcc agcaactcat ttatcataac ttatcgtttg ttatttgtca
      241 agaacttttt tgaagttttt ttcgatagaa tcaagcttcg caagtggttc ttttttaacg
      301 actcagttat cttatcatgt catttccaac atgtcaacaa cttatttttt attatttaat
      361 aagttattgg tgttcttttg aacaaataat aatatatcaa catcacatgt gaaagtcaat
      421 aagaaaatgc ttatttatca acatttctag cttacatgat cattggttag ttctggtcct
      481 ttttcgttcg tctttataac tccgagagac gtaaaatcct tttgttttca gtttaatgtt
      541 catgttcttg tcttattcac accaactaga tgtctcacct tctaaaattt tccttaacaa
      601 agtgctgctt ttatctattt ctgctttttt ttgctagaat aatagtaaca atatgactct
      661 ggaggattta tttatgaaaa ttttgattgc tgacgatgat aaagaaatcg ttgaattatt
      721 aagtatctat gttcacaatg aggggtatga agcagtaaag gcttatgatg ggaaagaagc
      781 attatctaaa atcagaacaa tccctgatat tgaattactt attttagata tcatgatgcc
      841 tgagatggat gggatgcaag tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat
      901 tatgctgaca gcaaaaacta ctgatatgga taagatcaaa ggactggtcg ctggtgcaga
      961 tgattatgtg accaagccat ttaatccgtt agaagtaatg gctagagtaa agtctttatt
     1021 acggcgtaca aaaatgcaga ttgccccaga tcagcctgat gtattagaag taaatgcatt
     1081 aatcatcaat aaagactctc atgaagttaa aacaatcgat gggaaagaaa tccagttaac
     1141 agctttggaa ttcggtatcc tctacttgct tgctagtcat cctaatcgag tatttagcgc
     1201 ggatgaaatt tttgagcgtg tttggaaaca agaaagtatt gtctctgcta aaactgtcat
     1261 ggttcacgtt agtcatttaa gagataagat tgaagaagca actggtggtg aaaaagtcat
     1321 tcaaaccgtt tggggagtgg gctataaaat tgacgcaaga tagtaaacga gcaactgaaa
     1381 aacgccgtcg gatcaatttg acttcaaaag aaatcagcga attgttagcg gaaggaatca
     1441 tcacgattat ccttttgctt ctattaaatg tttctattct ggttgttatc agttcggtga
     1501 tcaacagttc accttctttg acgaatgcga tttgggattc aaagaatatt tttgcagaac
     1561 gattgaacac tgatttgttc tggaacggcc ggaattttat tattcccttt ttctttctat
     1621 tagatattgg tgttttgtat tggcgcttga ttcgaagata tcgccaaatg caattgcggc
     1681 atatcatcag cgaacttcac tacatcgcaa acggaaatta tgatcatcgt atcccttttg
     1741 aactgagtgg tgacttaagc cgagtggtaa caagtatcaa tggattggtg gatagtaccg
     1801 tagcagcgat tgaagacgaa cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg
     1861 tcagtcatga tatccgtacc ccactcacgt ccatcattgg atacctagga ctgatagaag
     1921 atggccagta tcactcagaa gaagatcttt taaagtatac gcatactgct tatatcaaag
     1981 caaagcagat gaaatcttta gtggacgact tatttgaata cactaaggta cgccagcctg
     2041 ctgtcccggt gaatttttct gcctttgaca tgatccagtt gatcgaacag ttagcagctg
     2101 attttgagtt ggaagcttct aaaaaaaata tccaaatact tgttcaatca aaagtagact
     2161 cccttatcat ggatggcgat accgaaaaac ttgtacgtgt cttcaataat cttttgacga
     2221 atgcgttgaa atacggaaaa ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt
     2281 cagaagttgt tgcaacagtc aaaaataacg gagcaatgat cccacaacag gcaatcgata
     2341 atctattcga ccgtttttat cgtgtagaag aatctcgttc acaagcaact ggaggaactg
     2401 gacttggttt agcaatcgct caaagcattg tggctttgca cggcggatac atttatgcaa
     2461 aatcagataa gcaatggact tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat
     2521 tacctattga tacacaggaa gttattgacg aaagctgaaa tattcgctat tcttattaca
     2581 attattagaa taaatgaaaa acacagaacc cagtacactt tattcttttt cagagagctg
     2641 atggcaggtg taaatcagca aaggataatt tggaagttcc agttcgttta cgtgcccttt
     2701 cgaaataaga aaggtcggtt caacagccgt tatgtgttca acaagtgcag ctgacgctga
     2761 acttgggtgg taccgcgaat ttcagactga ttcgtcccaa gaataatggg atggatcagt
     2821 ctttttttat tttcaaaaag gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa
     2881 tattgaagaa gtacaagaaa aattgatgac tcggggcgta aaaaaagaaa cattagctga
     2941 attcatggaa ctagatgaaa atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa
     3001 atatcgaaat gatgtttctg ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc
     3061 tgctaaaatc gctgaaatga aagaagtcgg tggcaacatc aaagcattgg atactgaaat
     3121 tgctgaagtg gatgaaaaat tgaaacaaat tgcaactact ttgccaaacc ttccccatga
     3181 ttctgtgcca gttggaacag atgaagagga caatgtagaa atccgtcgtt ggagcgaacc
     3241 acgtacattt gcttttgaac caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt
     3301 agattttgaa cgtggcgcaa aagtagccgg tagccgtttt gtctattaca aaggattagg
     3361 tgctcgatta gaacgtgctg tctacaactt catgcttgat cagcatattt acgaacacgg
     3421 ctatactgaa atgatcactc cttatatcgt caacagtaaa gcgatgtttg gtacgggaca
     3481 atttccaaaa ttcaaagaag atgttttcca attggagaat actgatctaa cattaatccc
     3541 aacagctgaa gtaccattaa ctaactatta taatgatgaa attttagatg gtgcagattt
     3601 acctatttat ttcactgcac taagccctgc ttttcgttct gaagccggaa gtgctggtcg
     3661 ggatacacga ggactgattc gtcttcacca attcaataaa gtagaaatgg tcaaattttc
     3721 ggatgtagag cattcttacg aagaattgga aaaaatgaca gctgatgcag aagatatctt
     3781 gaaaaaattg catttgcctt atcgtgtgat ggccttatca actggagaca tgggcttttc
     3841 tgcagctaaa acttatgact tagaagtatg gatacctgca caagatactt atcgtgaaat
     3901 cagttcttgt tctaactgtg aagatttcca agcacgccgc gcaatgatcc gttatcgcga
     3961 taatgatgga aaaatccact atactcatac tctgaatggt tcaggacttg cagttggccg
     4021 tacagtagct gccattttgg aaaactacca aaatgaagat ggttctgtca cagttccaga
     4081 agcgctcgtt ccttatatgg ggaatctgaa agttattaaa taaagaggct gtgaaaaagc
     4141 cgcttagttt cgagta
//
LOCUS       DACBZN010000164         4150 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.167, whole genome shotgun sequence.
ACCESSION   DACBZN010000164 DACBZN010000000
VERSION     DACBZN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.167"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            362..805
                     /gene="rplM"
                     /locus_tag="IVD64_002754"
     CDS             362..805
                     /gene="rplM"
                     /locus_tag="IVD64_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP6149957.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            821..1213
                     /gene="rpsI"
                     /locus_tag="IVD64_002755"
     CDS             821..1213
                     /gene="rpsI"
                     /locus_tag="IVD64_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6149958.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1302..2528)
                     /locus_tag="IVD64_002756"
     CDS             complement(1302..2528)
                     /locus_tag="IVD64_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6149959.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2600..2758)
                     /locus_tag="IVD64_002757"
     CDS             complement(2600..2758)
                     /locus_tag="IVD64_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6149960.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3446..>4150
                     /locus_tag="IVD64_002758"
     CDS             3446..>4150
                     /locus_tag="IVD64_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6149961.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         1349 a          668 c          722 g         1411 t
ORIGIN      
        1 agcctgccgt tcagctccga gtcacaagaa acaattttaa aaaacagctt cttatgtttt
       61 atggatattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcaaaataag
      121 gggttgtgac aagacttttg tcacagcctc tttttgtttt tcattttgac aggaaacgac
      181 ccttttatat aaaagaaaga aaagaagtaa agtttcatat aaatttttca atcaagcaga
      241 aaaacgttga cattgactag attagcgggt aatatgttaa atggtattgt ttgccccaca
      301 aagagccccg gaaactcgaa gtgtatgtca aacatcagaa cttaaattgg aggaaaataa
      361 cgtgcgtaca acatatatgg ccaaaccagg cgaagtagaa cgtaaatggt atgtagtaga
      421 cgcaactgat gttccattgg gacgtctttc aacagttgtt gcatctgtac tacgtggtaa
      481 aaataaacca actttcacac ctcatttgga tactggtgat ttcgtcatcg tcatcaatgc
      541 agacaaagtg aaattaacag gtaaaaaagc gactgacaaa atttattacc gtcacagcaa
      601 ctacccagga ggattgaaat caatcactgc tggcgaattg cgtgctaaaa attctcgtcg
      661 tttgatcgaa acttctgtaa aaggtatgct tcctaaaaac actttaggcc gcaaacaatt
      721 tacaaaattg aacgtatacg gtggagcaga acatccacat gcagcacaac aaccagaagt
      781 tttagacatc acaaacttaa tctaatagga gggaatttca ttggcacaag ttcaatatat
      841 cggcacaggc cgtcgtaaaa acgcagttgc tcgcgtacgt ctagtaccag gaactggcaa
      901 aattactgta aataaaaaag atgttgaaga atacattcca catgctgact tgcgtgaagt
      961 aatcaatcaa cctttcgctg ttactgaaac aaaaggcgca tatgacgtat ttgttaacgt
     1021 aaacggcgga ggatacgctg gacaatcagg agctatccgt catggtatcg ctcgtgcatt
     1081 gctagaagtt gatcctgact tccgtttagc tcttaaacgt gctggcttac ttactcgtga
     1141 cgcacgtatg gtcgaacgta aaaaaccagg tctcaaaaag gctcgtaaag catctcaatt
     1201 ctctaaacgt taatattatt tatatatcaa tgtttcaagg cacttttcaa ttgtttgaaa
     1261 agtgtctttt tcttgtttaa gaacacattt aagaacacat actaatcaca tttattcaat
     1321 cctctctgaa aggcttcaaa aacagtttta tctactacat ctggtgtatt tacgtatatt
     1381 ctcgttgtag aaatatctga gtgtgttaaa gcactagata tttgattcat atttgctccg
     1441 ccttgtctag ctatagttgc ataggtatga cgaagcttat gtggagataa atgagccaat
     1501 tcaggatgtc gtcttttaat tgaattgatt cgatagttta ggtaatcagc gtgaacaggg
     1561 caatttactt ctccggatgg tttagtatag gtaaagagat actgatctgg tgtttgatta
     1621 atttttaatc tcaacaattg ctcagcttgc actgatttcc atttgcttag taatgccatt
     1681 acaatttcgg gtacttgaat tacggtatcc tttcgacctt ttgtaaattt ctttttttga
     1741 tatttatcca aagcatgttt aaggcgaacc gtttgtttct caaagtcgat atatttccat
     1801 tgcaaagcat acgtctcact ttttctatca ccaagatata gggtaagcat gaaaagagta
     1861 tagtcatgaa gagtaagcaa gtgattattc aagtcagtgt ttactgcttc tatccaagca
     1921 ataagctctt ctgacgataa tgcttgatta ccttcccgta tccttctttc ttctaaagca
     1981 atttttttag gtgcagtgat acattgaata attttggtag ttcggttaaa ttcaatataa
     2041 tttagtattt ctgcaatatc aaacatagaa cggacatatc ctttgataat ttttatattt
     2101 gcgtattctt tcgatttttt tgttaattca tctgatatta tctcagtatt tgaatttaga
     2161 tagttcattg catactcacc aaacattgga aggatgtgca atcggaatat atctttagta
     2221 ttactaattg ttacgtaact tggagctctt tttgatctag tagtctgacc tagttcatat
     2281 ctaggaagcc attttgattc atagaattcc ttaaatttta tttttccttt taattctagt
     2341 gaatttgcat tctcctctct taaaactgtt tcaattttcc ttttcattgc attttcggct
     2401 ttaatagcca attgcctagt tggaaatact ttttgatact tttctgatgt aattccagtg
     2461 atttctctga atttttttgg atacctcaga ttagttttat atttcccatt tgaaagtttt
     2521 gtaggcaaaa ttcctcactc ccttaaaaag taaggcagta tatttcgcta ttcttagtat
     2581 atgcggcgag aagaaatttt caaattgttt taaatgtatt cataaaatct ttgatatcct
     2641 ctatgtcaaa gcgaactata ccgtcaattc tgattactgg aaaattatat atacgaatcc
     2701 acttatctaa agtattatta tcaattccta gaaactcaca ggtttgtttt ttattcaagt
     2761 agactctcaa ttttataaaa tcatctccta aaaaagatta gaatttttga attgctttgg
     2821 gtaagcacat aggaatttca cctctcccca gtttacggcg agccttacgg aagtatcatt
     2881 atttgctgct attgtcatgg cattttgttt tcccagaaca aaaagaaatc tctaagtatt
     2941 tgtcgctaat caggtcatgg cgtacttgag tttctagctc gaatagcagg gatcgttatt
     3001 caaaaattgt atttaattgt caatgatcaa aaataggttt tttcctattc actattaata
     3061 acgataaaca tgtataaaaa gacacactac gaaaaaagtt aaatggagtt tactgatttt
     3121 catatttgta tatggaacag acatagtctt gaataatttt tataaatgtt ttgatgctta
     3181 tcaacttatt caacccgcta tccactttaa gtataatttt tccatacgcc aataaagtag
     3241 ttttaggagc atgttctcat ttgagaacgc tcctttttta tatattttga aagctactta
     3301 ctatacgcgt agtttcaaat agttttgtca ttctaatctt cagcaacaga aaaaaatcta
     3361 aattataaaa tttagggttg actaaaatat aatttttgta taccatatga tcgtgataat
     3421 cattatcatt tagaaggaga aagttatgtt agaagtgaaa aagcttaatg tttgttataa
     3481 cgatttttta gcattgaacg agatttcttt aaaaatacaa gaaggtgctt taacaggtat
     3541 tattggtcca aatggtgctg gaaaatctac attgcttaaa gcaatgttaa atattatccc
     3601 tcatcaggga gaggtctcca ttaataatga agatatcaat aaaaaactgg tgaagattgc
     3661 atatgtagag caaaaagctg atattgattt tacttttcca ataaaagtaa aagaatgtgt
     3721 atctatggga acatatgccg gaatgaaagt ttttcaaaga attaaaaatg cagaatggca
     3781 aagagtttca aaggctttgg aaaaggtcga tatgggaaaa tattctaatc atcaaatagg
     3841 agaattatct ggaggacaat ttcaacgtgt acttttagca agatgtcttg ctcaaaatgc
     3901 ggatttcata tttttagatg aaccatttgt agggatagac ttagttagtg aacgaataat
     3961 tatggatacg ttaaaagaat tgaaacatca gggaaaaaca gtgctaattg tgcatcatga
     4021 tttaagcaag gtaaagaagt attttgataa tattattctt ttgaatcgaa atctaattgc
     4081 tcacggaagt gtagaatctg tatttaatga agagaattta aaaaaagcct atggagatac
     4141 tatatttatt
//
LOCUS       DACBZN010000165         4112 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.168, whole genome shotgun sequence.
ACCESSION   DACBZN010000165 DACBZN010000000
VERSION     DACBZN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.168"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(39..281)
                     /locus_tag="IVD64_002759"
     CDS             complement(39..281)
                     /locus_tag="IVD64_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6149962.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(313..870)
                     /locus_tag="IVD64_002760"
     CDS             complement(313..870)
                     /locus_tag="IVD64_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6149963.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(883..1071)
                     /locus_tag="IVD64_002761"
     CDS             complement(883..1071)
                     /locus_tag="IVD64_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6149964.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="IVD64_002762"
     CDS             complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="IVD64_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6149965.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2880..3353
                     /locus_tag="IVD64_002763"
     CDS             2880..3353
                     /locus_tag="IVD64_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP6149966.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3362..3589
                     /locus_tag="IVD64_002764"
     CDS             3362..3589
                     /locus_tag="IVD64_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6149967.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1234 a          740 c          617 g         1521 t
ORIGIN      
        1 ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat
       61 gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca
      121 attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca
      181 atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct
      241 ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg
      301 acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt
      361 gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc
      421 tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt
      481 gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc
      541 agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac
      601 gatcacatct agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt
      661 tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt
      721 caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc
      781 ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc
      841 atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg
      901 atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat
      961 tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc
     1021 aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc
     1081 ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct
     1141 gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc
     1201 gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc
     1261 gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat
     1321 gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt
     1381 tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac
     1441 gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg
     1501 ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat
     1561 atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat
     1621 aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc
     1681 tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta
     1741 aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg
     1801 aaataacaat tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat
     1861 attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta
     1921 tttattaaat tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc
     1981 gttcttactt tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc
     2041 ttataaataa aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa
     2101 cgattggcta tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat
     2161 tcagttggac tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac
     2221 aaaattatcg aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta
     2281 tggtactttt tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac
     2341 ttttgattta taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga
     2401 tagtaccacc ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata
     2461 aaggacaaat aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa
     2521 taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct
     2581 catacagttc tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta
     2641 ttgtccaata taatcccagg catctaaact agcattatag aagagacaca catagaatct
     2701 aaaataactg gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac
     2761 cttcatactc tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag
     2821 ttgttttaca ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa
     2881 tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt
     2941 tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt
     3001 ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg
     3061 cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa
     3121 gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg
     3181 gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg
     3241 taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa
     3301 aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga
     3361 catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga
     3421 attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc
     3481 aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg
     3541 tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga
     3601 acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt
     3661 acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat
     3721 gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca
     3781 actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca
     3841 ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt
     3901 tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat
     3961 tcttttttag taagctccca tatatactgt aaagccttct atatattatg tcatgttgct
     4021 ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct
     4081 tttttatttt taacattatt ttgataataa tg
//
LOCUS       DACBZN010000166         4104 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.169, whole genome shotgun sequence.
ACCESSION   DACBZN010000166 DACBZN010000000
VERSION     DACBZN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.169"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..751)
                     /locus_tag="IVD64_002765"
     CDS             complement(<1..751)
                     /locus_tag="IVD64_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAP6149968.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYK"
     gene            complement(1393..2064)
                     /locus_tag="IVD64_002766"
     CDS             complement(1393..2064)
                     /locus_tag="IVD64_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6149969.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            2587..3177
                     /locus_tag="IVD64_002767"
     CDS             2587..3177
                     /locus_tag="IVD64_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP6149970.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3367..4080
                     /locus_tag="IVD64_002768"
     CDS             3367..4080
                     /locus_tag="IVD64_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149971.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1320 a          714 c          598 g         1472 t
ORIGIN      
        1 gtttatatat ttttgttttt gagaagcaaa actcctcttc gttaacatat tttgcagcca
       61 tttttttatt ataattacta acttttcttg cttcttcctc taatttagca tcagataaat
      121 tacgaatatg tctagtttga ttttcttcct ggttatatat atctttatct tcaatcaatt
      181 gctcttgatt tattaaagct aatggaaatt tatatacttt tctgctgttg cccgaactat
      241 ctttttcatt aacagtatat atttgtcctg ctaaatatac ttctcccaga tacgtgtgtt
      301 cttttggttt aaatgtttca aataaatgaa tctttacaaa attacttttt gaattggcta
      361 aaactttatt ttgattctta tccaatactt gatccccttt tagccccata ccggtatatt
      421 gtaaaatacc atttaaccac ttatctttat aaacattact tcctgtttga tcactaaaca
      481 gtacaagagt attagtttga tgagatcttc tcattccacc ctgtttgaaa catttaaact
      541 tctctgaaat ttctttgtta gtatatactc taccaacttc taactcacct aatatgtttt
      601 tagaccaatt ttctcgccat gtaataatct tatcaagatt aaacatttct ccctcttttg
      661 gcatgccatt tctaattaaa tcagctaata caccagtaga aatatctaat ttttttagta
      721 attctttctt agtaatcaat gttggattca taacaatgca gctcctaatg atatcaatat
      781 aattgcatta gataacttga aaaatcgaaa aaaactcatt ttgtgtcaag ttatctgtaa
      841 tatataaaaa catcattatt atacctcatc tatgtgaatt tttactattc ttaccttagc
      901 atattcttta taggtatatc agagctaatc aaactaaaaa ataccccaag tcttcttatg
      961 taaaaacacc ggttattact taaatatgac ttaggtaaaa caatgagtga tccaattata
     1021 aaattttatg gcgaataatt tagtaagcgc tacatattga atgatttaga aattttgaaa
     1081 aagtagctag ttccttttat accagaaaag taaacctaat aaaaaacaca actaattttt
     1141 agctgtgttt tttaagttac aataattatc tatttaatag tcttaattca ttaaaacaaa
     1201 aaactcaact ttacaaggat atggtttaaa aaatttattt atccaaccca actagttttc
     1261 gctgcttacg atttgcttca tctgctacgt acttcattac tgttttaaat tctttcgctg
     1321 aggcgttttc tgataaatca gcgatacttt tattatactt cttcacgatt tgttgtactt
     1381 gttctttctc agtcatgtaa ttcctcctcc aactcttttg aagtatattg tattgggtag
     1441 tactaattga atattatctg ctgcataatg cacttcagaa atgtcgtttt gataatcctt
     1501 aatcattatg tgaaggacta cttcaggatg tctatttttg actttttcaa tattctctct
     1561 ggacttaaaa aacgcattga taaacatttc ttttggcaca tgacgtcctt cagctagttc
     1621 acgttttcga gtgaaatccc aggcaacttt gggatcttgg taaacataga aaatcgtaac
     1681 gcgaaagtta tttttaaggg ctcgaataat atttttctct gcactaagga tagcaaaagt
     1741 atcctctagt atgaaagaat agccatgttc cagtacatat tttaatgatt gttcaactaa
     1801 ccaagaagaa gctttctgaa aaacacttga gttctttcca ttgtattctg gaaattgtgc
     1861 tctaaaatag tccgcatcaa tcacaacatg gttgtcataa ttttcagcta aacctagtgc
     1921 aacctctgtt ttacctgctc ctgggcttcc agccatgaat accgcatctt tttcatcatc
     1981 agggattttt cccttgatga ttgtttcaat aaatttatct ttattgaccc tagcgtaatc
     2041 taaatacttg tctttaggtt ccaagattgc accttctttc cttcttctat attcatacta
     2101 attatacgat atttatataa aaaatataac ggtcatttta accatattaa catttagtca
     2161 cagtaatagc agatatgctc gcaaaatgag atatgaatgt acgcagaatc cttgttttct
     2221 acggcattcg ctgttttttc attggactag ttatatattt ctagggttgt tttcatgtct
     2281 gagtgcccga tatgatttat ttttcgtttc tataattact ttttgccttc cagtttcttt
     2341 taagattgta ctcaagcaag tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt
     2401 ataatgctgg taaacaaaaa tgattacctg tcatttttat ttagttcgta tttaattaag
     2461 ttgactgtct gtatactctg tctaaaactt aagaattgtt gttttcgctg tcgctatcca
     2521 aatttaatga atgtctgtat gttaaaactg ttgtccttgt catagatagt aaaggagagc
     2581 ttgaaaatga aaactgcgat tacgtttcta ttagaggaat atgccgattg ggaaggtgct
     2641 tatattacta gcacactaaa ccaaaaagag ggatggcaaa acaaaactgc ttctatccaa
     2701 tcagaggtca cttccattgg aggattaaaa acaaaaatcg attacttaat cgaacaaata
     2761 cctgaaaaag ttgacctttt tattatgatc ggtggcaatt catgggacat aaaaaatcat
     2821 catttatatc aaataatcga taaattgtta aaaacagacg taccagtagc tgctatttgt
     2881 ggggcagttg attatttagc tcagagcgga ttattgcaag gttacaaaca caccggaaat
     2941 gcgcaatttc cttggttaga atcggaaaat tatcagaata aagaagattt tatagaacaa
     3001 caagctgtga aagatcgtaa tttgattact gcgaatggaa cagctcctat agaattcaca
     3061 gacttgattc ttcaagcagt ccattttgaa acagaagaaa taagagaaaa aacgattcgt
     3121 ctatacaaac ttggattcta tgattactat aaaaagtacg gagatccttt ctcctaatca
     3181 ttctcttcaa ttaaatactc cttctcaata gtatgacaag taattttact gatggttatt
     3241 tgtcacactt tttctgacat ttatcaaaga agatatactc tatttatatt catagacaag
     3301 gttttacgga ttcaaaaatt caggattacg ctatactggt attaaaaaaa taatgaggta
     3361 ttttatatga aaaagttagg ttttatttta ttgttggtct ctttattcct tgtaggatgt
     3421 acgtctccta gtaaagaaaa tagtgatcca tctgcgacca cagacaaaag ttcaatcaga
     3481 caaaccagtt cagctgaaaa atcaagtact cagaaatcag cttctactga aacgaataca
     3541 tcttcaacaa gcagtaaagt ctcagagccc aatacttctt ctacatctag tagtacagtt
     3601 gagacaaata tttcttcttc aacaatagac acaacttcct cacagtcttc tcagtcaata
     3661 gatccgttag caggatattc tgatctacaa attgagtacg cacgtgtttg gctagctatt
     3721 agaggtaata cttttagaga tacatcgagt gatttcgaac tacatgttat tcattatcct
     3781 gcaggaacac ctatcgcccc ttatgatgca gggagtgcag tctatccgaa agatgtggtt
     3841 atgctcactg ggaaatacag ttaccaggga gttttagtgt attcaagtaa ccatgacggg
     3901 acaatcacta gttatccagt tcctacacat tggcaaatgc ctgcagatca aacatcttat
     3961 ccagcgttca ttagagaaac aactcaagaa attattgata atgcatctgt agtagctatt
     4021 cctacaggta atccaaatga tgtgaaacag ctgatcaatg tgatagttat ggataattaa
     4081 aatttctaat agatcataca aaaa
//
LOCUS       DACBZN010000167         4073 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.170, whole genome shotgun sequence.
ACCESSION   DACBZN010000167 DACBZN010000000
VERSION     DACBZN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.170"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..938)
                     /locus_tag="IVD64_002769"
     CDS             complement(<1..938)
                     /locus_tag="IVD64_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6149972.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKD"
     gene            complement(1160..1522)
                     /locus_tag="IVD64_002770"
     CDS             complement(1160..1522)
                     /locus_tag="IVD64_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149973.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1526..1834)
                     /locus_tag="IVD64_002771"
     CDS             complement(1526..1834)
                     /locus_tag="IVD64_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149974.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(1843..2262)
                     /locus_tag="IVD64_002772"
     CDS             complement(1843..2262)
                     /locus_tag="IVD64_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149975.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2255..2488)
                     /locus_tag="IVD64_002773"
     CDS             complement(2255..2488)
                     /locus_tag="IVD64_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149976.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2491..3459)
                     /locus_tag="IVD64_002774"
     CDS             complement(2491..3459)
                     /locus_tag="IVD64_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6149977.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QEGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(3906..>4073)
                     /locus_tag="IVD64_002775"
     CDS             complement(3906..>4073)
                     /locus_tag="IVD64_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6149978.1"
                     /translation="AFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQR
                     KLERSEDEIEW"
BASE COUNT         1154 a          928 c          569 g         1422 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcgctctctc agaagccatc agagccttat cacgctcgtt ttcttctctt
      121 tgcctagaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac agccgtttgg
      181 cgcaattcag aaatttcact catgatttct ttgtattcgt gaatatcttt aattttgttg
      241 gtaccgacaa tatccctttt ccaaccgtaa tcattcatta atcgttctat agagtcaact
      301 tcttgatttc taaaattatt gaaaagcact cgaccattat ctttatcagc gtgtatccct
      361 tgttgaacca gtgctttatt aaagctcggc tgcttttgaa caccccgttt ataaccttca
      421 gcaaccggca caatattcaa gtgcaggtgc ggacttattt cgtcattatg aataacagca
      481 ttataaacag ccaaattagg gtttctctct tgaaactcct tgtaatactg aaccaaaatt
      541 ttattggcaa tcactcgatt ttgttcagct tgttgtttcg atatgccagt agaagaacca
      601 tctgctcgat ttgtctggaa atcatctaaa atgcctattt gcacaataaa ttctcgttga
      661 ggttccaact ttttatcctt gcgcactttc gctaaatagt cctcgatttt tctatcggct
      721 cttttttgtt tcgcattata tttttctacc acttcgccaa acaattcctc atatttatct
      781 gctagattcg tctgctctaa ataaatattt tcatccgatt tcgtgaagtc aatatgctta
      841 tgccaatcat tggttttctg gcgatcagtt aactcacgat tattatgaga aatacttgta
      901 ttcttcgttc cttttttaaa tgaaatacta aaccccaagt taaaaccctc cttcgcgctg
      961 tcgctaataa aaagattgta aacctttctg ttactctttg tcaaagagta acattttcct
     1021 aattgtgaca tggctgtcac aattccaaat gctctccgag ggtattcgct gtcacaagtg
     1081 acaacgaaaa aaggagccga aagcgtgtca cgctctcaac tccttttacg ctttattttg
     1141 cttcacgcaa aataatcgct tatcgtaaat tcacgtcatt aaatattgac gtaaatttac
     1201 tctcactcat ctgacttttc ttattcggta tcactccgaa taagccgtca gattcatcgg
     1261 gataatctcg ttcgcttggc gctgattttt catcagcaag ccactcatta tccactgatt
     1321 tttcgtgttg ctgatcgtca gaaattgacg ataaccaccg ctcaaaatca tcaaaagatt
     1381 caaatccaaa atgcttccga agtgccttac ccatttcagc attttccttc tcaatcaatc
     1441 gttttttctg ttgacgaagt tttgcctgtt cttttttcaa tttttcttct tgtgctttaa
     1501 tatctgctaa agtcattccc ataaactaca tctccttttc tttggaatta ataacggaaa
     1561 tttggtttaa ctgcataaca tgactcatta atttcaacca aaattctttt tctgattcat
     1621 cagttgtttg cgctaaatgc ttatgaacaa attcattagt caaaactgaa gattttgcta
     1681 gatcagcatt gataaccgag ttgagaacat ccatcattcg ttcaagcatt tcactataat
     1741 tatcttcatc cggcaaattc aaataatcag ctaaccgttc atacatcaat tcaattctat
     1801 cgactgtttc ttctctcaaa tcaattttat ccatgtcaca cctcacttcc agaattgcca
     1861 ccattttcga ttttttaatt cttgattttc tagtaacaac tgatcgtttt tttgttgctg
     1921 aagcaaagtc aatttttgtt ggtcacgaat catttccgtt aattcagaaa tatgcctatc
     1981 tttttccgct aattgttcac gcaaaaattc attttctttt tctaacggga aatcattgcc
     2041 tgcatcattg accggtaaat caccagtcaa ttgaccacct tttgaccgat aaattgaccg
     2101 cacaacacca atcaattcct tgtctaacat gataacccca ttatctttat accgagcaag
     2161 gtcataacct gcattgacca gttgattgcg cactgtattt ttagatatac ccaactcatc
     2221 agcaaggttt tttatggtca ttttttctgg caaatcagtc attatcttct ccgttacttt
     2281 cagctacccc ataaacatat ttaacagcat gcttagggat aatgattggg gaatcaattt
     2341 tattattttt caaaatcaac tcatactgcg ttacatcaag taacttaccg gacatttttt
     2401 tgctatccaa aaaaacaacg acaacgttat cacctgttaa attcaccaac caatttttct
     2461 taccaaaatg cttttgtttt tctgccattc ttattcctcc tccgttacat ctaacccttg
     2521 aagccaagtg tctaattctt ttttcaaaac atctgctgat tccgaaaatg tttctcgtaa
     2581 atgattcgtc aactcatcat tttgactttc tcttttcaaa atatctctca atttgtattt
     2641 cattctacgc tctaaaaacc cccacatttc agggttagca atcagtccaa aaatcatcac
     2701 cttatcattt gtttttacat caagtggtag aaaatgaggg ctagcaaaac tatttaaact
     2761 ttcatgaacc attgcatgcc aatctgttgc tttcccacgc cttaactgca attccagtcg
     2821 ccaccaagtt tctatttctg gtggcacaat tttacgcttg gtttcctgtt ccaatttttt
     2881 gttgtatagc cgaatctggc gttcgctggc acgactgccc caataagctg tttccagctt
     2941 cccagaccgc ccataaattg gcttgaaaga aacaggatca accactcgat attgtgtaat
     3001 aaactcatca ggcacatcga taatatcgca agcaatatcc gcacgtgaga aatgtggttc
     3061 tagaaataaa tcatgaatga aatttttcat tgaactagct aaaaaatgac caatcttatt
     3121 cgggttaaaa tcaatccgcc ctttcccctc ttgccatttc aaaatctcaa tataagcaat
     3181 attttcttcg tattggtcta ataattgcca gccgttattc ccaaccgctt ttaaatagcc
     3241 attgccttca ttcacccgca ttaattgctc aaaatctaaa atcaacacat cattttgagt
     3301 gtgataataa atattttcct tcagttttcc aacgatagta attcggtcaa ttgaccactg
     3361 catttttaag ggggtgtaca tattttgtac atttaaccgc ctgttagata gggctgttaa
     3421 attttcccac tcatttacga ttatgccatt tttactcaaa ttttgttcct cctcaaagaa
     3481 aaagagactt ctggaaagaa ttttcaaaag ttcacgtaac tcttaaaaat tcattaccaa
     3541 aaaatctcta gtcttgaaga aatctaaaaa tattcgtata atgattaaga agttacggag
     3601 ctagtgcgat tagtttccgt aaaggtcgag aacgttggta gcgttgctcg actatttttt
     3661 tactcttttt tcagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc
     3721 aaatatccaa aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc
     3781 taggttggcc aaaaaatgga ctggccattt tttagcaaat gtcagcttaa taggctagtg
     3841 gtttagccgt caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat
     3901 cgtaactacc actcgatctc atcttcagaa cgctctaatt tacgctgtga gacgttttga
     3961 cgataaaata aggttttctc ctgctctaac acttctaatt cctcagaaag ccccatatcc
     4021 ttttctgcaa gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgc
//
LOCUS       DACBZN010000168         4050 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.171, whole genome shotgun sequence.
ACCESSION   DACBZN010000168 DACBZN010000000
VERSION     DACBZN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.171"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(91..1557)
                     /locus_tag="IVD64_002776"
     CDS             complement(91..1557)
                     /locus_tag="IVD64_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6149979.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(1848..3932)
                     /gene="cadA"
                     /locus_tag="IVD64_002777"
     CDS             complement(1848..3932)
                     /gene="cadA"
                     /locus_tag="IVD64_002777"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6149980.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1223 a          863 c          643 g         1321 t
ORIGIN      
        1 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
       61 tcacaacctc ttgtaacaag tatttctatt ttaaggctga accactgttt ctggttcagt
      121 gatttctttg tttttgtctc gacgagcaat tgctggcaag atcataccta ataagatcaa
      181 gacgattggt gtgatgatat tcgtaaataa ttggaaccac catgctttag gatcagcagt
      241 ataatctact tttggcacca ttcctaggat acatgcaaac gcagtgaagg caaagcacca
      301 agcaccaaag ataaaaccga tttttggatt tttaacaaat ttatattcag aattgaattt
      361 cttataagct ttattcaaca tcatatatgc caagaacacc cataggtaac gcattggcat
      421 gacaactgag ttcaagttcg tcaaccattt tactaattca ttcatatctt tgataccaaa
      481 aatcggcaac aaaataatga ttcctactag tatacctgtc agaagataac cattcaccaa
      541 tgtaccttta cgattacgtt tacgtagcca gtttggtaca aattctggat ccgcatctgc
      601 taacaagatt tttagtggtg catcaattga aaaggctaat gcggagattt gtccaatcat
      661 gtttgtcaat gcgtaaatga tcatcaatga gttaccgata ccatagtaat tacctaaaat
      721 ttgaaaagca ctgtatgccc catttgccat caagtcttta ggaatattat cactagcaaa
      781 cagcataccc attgcgactg agcctaatac agcacaaatc cctaccattg ctgctaagaa
      841 gatcatccct ttaggaaatt cctttgcagg atttcttgta gagttgacat aaggggatat
      901 tttttccgcc cctccaactg caaatactag catcgaaacg gttgtgaaat aactgaaatc
      961 aaatgttgga atatatgttt tcacttgatt catatgtggt gtggcaaatt ccatcgttgg
     1021 attgatgaac ggtgccgtta cagctaaaag aataaataag atcgacatga cgaacatcgc
     1081 tgttccggcc agtccgccaa tcactttcaa agtagaaagt ccttttgttg ataaatataa
     1141 aaaggcaaga aatataataa gtgaaataac tgctaccatt tgtactgaca tcgtatttac
     1201 gatatttcca tttccttgga ctgcccaacc accagcaatc agaattgctt gaggcttttg
     1261 tgccaaataa ggaatatgga cgacccagta agtccaagcg gcatagtatg ccagtcgttt
     1321 attagatgtc gtattttcga cccatgaact gacgcctcct ttactatcct tgaaggctga
     1381 tcctaattgt ccaacgatca atgcgtatgg aataaaataa atggccaaaa tcaatagcca
     1441 agaggtgaca actgatactc cttgctgagc atagttatta actacattcc ccatgcccca
     1501 aaccatgtta aatgcaatta acgcaacagt aaaccagcgt aattgtttgt tgttcattcg
     1561 ttctctcctt tatgataaaa atttgttatc cagatgtaat catattcaat ttttaacaaa
     1621 cttacaagac agtgttattt tatgtgtgta ttcttttatt taatacacat cgttttttca
     1681 tattttttcc atcaaaaaaa taagttattt tcttttattt cattgacctt attgaatttc
     1741 aagctatttt gaattttctg caaaattaaa aacattagtt attttataat cctatctatt
     1801 ttctttacat ttttcattaa gaaaaggata taaaaaaaag aggacgttca ttcgtcctct
     1861 ttttttacat gcaagcttcg catggcattc aaaacagcta atactgtcac cccaacatct
     1921 gcaaatactg catctcccat tgatgcaaaa ccaaaagcac ctaaggcaag taccaaaatt
     1981 tttatcccaa tagcaaagac aatgttttgt ttgacgattt tcaatgtttt ctttgctaaa
     2041 tggattgctt ctgcaatctt cgatggttga tcattcatga tgactacatc tgctgcttca
     2101 attgcagcat cgcttcccaa tccacccatt gcgattccta catctgctct tgctagcacc
     2161 ggtgcatcgt tgattccatc tccgacaaat gcggtctttt tattgtttct ttgtaagatt
     2221 tcctctaacc gctgcacttt atcttcagga agcaattcgc tatatatctt gtccacgcct
     2281 acttgcttcc cgatatgatc agcaattttt ttcgagtcgc ccgtcaacat gactgtgttc
     2341 ttcacaccag cctgttttaa ttgaaccaat gcgtctacgg catcttcttt caaggtatct
     2401 gcaataacaa gataaccact gtaactttga tcgatcgcta gataaagcaa tgtaccgatt
     2461 tcttgactta aaggaaaaga gatttcaaat ctttccatca atttgtgatt acctgctaat
     2521 acttgtttgc catcaacttc agctaagatc ccttcacctg caaattcctg gatattagtt
     2581 gttggtaaaa gcggacctgc gtagccttct aaaacagatt gagcaatcgg atgtgtagaa
     2641 aactgttctg cactcgcaat atattgcatg aatttctttg gatcgatttt tgtatcgatc
     2701 gtctggatcg aaaagtcacc tttcgttaat gtgccggttt tatcgaatac cactgtctcg
     2761 gtttgcgcaa gcaactccaa gtaatttgaa ccttttacta atacaccgat tttacttgcg
     2821 cctccgatac cgccaaagaa actaagtggc acggaaatca ctaatgcaca tggacatgag
     2881 atcactaaaa atgtcaatgc tcgatagatc cattcagaaa acgccgctcc agaaacaaca
     2941 agtggaggta aaacagctaa taaaacggcc aatcctacaa cgactggtgt gtaatagcgc
     3001 gcgaatttag taatgaaatt ttcagctggt gcttttttac tgctggcatt ttctactaat
     3061 tctaataatt tgctgatagt agaattttca taagattttt ctacttgaac taaaaatggt
     3121 ttatttttat tgataaatcc gcttaatact gtttcaccaa catatacaga tttagggact
     3181 gattctcctg taagtgctga cgtatcgact aatgattctc cttcgatgat cgttccatct
     3241 aacggtacac gttcaccagg cttcacaagt acaaactgac cgatctgcac gtcttcaggg
     3301 ttcacggtca tcaatccttc cgtcgtctgc acgtgagctg tttctggacg gatcgccatc
     3361 aattcgcgaa tcgattttct tgaatgactg actgccaatc cttggaaata ctcgccgatt
     3421 tgataaaata gcatcactgc tacggcttct ggatattcac cgatcaacat agcgccaatc
     3481 gtagcaacag acataagaaa attctcatca aagatttttc ctttgacgat attcatcact
     3541 gccttcttca ctacatcata tcctatcagc agataaatca ataaataccc tatcaagcga
     3601 attggttcat taaaatcaaa taatagcaat aaagcgatac ctgcaaggga aaataaaatc
     3661 cggaacagcg tatttctctc tttttcattt ccatgttcag atactttttc cttatctatt
     3721 tctgttactt tgacatctgg ttctaattcc ttcactactt tttttgtttc ttgttcaatc
     3781 gtagccaaat gatcttcctc tgcgtcaatc gttagttttc cagaagtgaa gtttaccgtt
     3841 gcttccttga ctccattgat cttctgaact cctttttcga ttttcattgc acagttcgca
     3901 caatctaaac cttctaatct gtaacttttc attttaatga cctcctcgcc tcaacatatg
     3961 aacatctctt cataactaat tataaatgaa tggttattca tatgtcaagg tatccgaaag
     4021 aaaaaatgaa aaacatctgc atcggaaaat
//
LOCUS       DACBZN010000169         4034 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.172, whole genome shotgun sequence.
ACCESSION   DACBZN010000169 DACBZN010000000
VERSION     DACBZN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.172"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            79..234
                     /locus_tag="IVD64_002778"
                     /pseudo
     CDS             79..234
                     /locus_tag="IVD64_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..1530
                     /locus_tag="IVD64_002779"
     CDS             253..1530
                     /locus_tag="IVD64_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6149981.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="IVD64_002780"
     CDS             1540..2196
                     /locus_tag="IVD64_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6149982.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="IVD64_002781"
     CDS             2196..3572
                     /locus_tag="IVD64_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6149983.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="IVD64_002782"
     CDS             3702..>4034
                     /locus_tag="IVD64_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6149984.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DACBZN010000170         4015 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.173, whole genome shotgun sequence.
ACCESSION   DACBZN010000170 DACBZN010000000
VERSION     DACBZN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.173"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..168
                     /locus_tag="IVD64_002783"
     CDS             <1..168
                     /locus_tag="IVD64_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6149985.1"
                     /translation="AFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQH
                     KLERSEDEIEW"
     gene            768..1757
                     /locus_tag="IVD64_002784"
     CDS             768..1757
                     /locus_tag="IVD64_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP6149986.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1754..2173
                     /locus_tag="IVD64_002785"
     CDS             1754..2173
                     /locus_tag="IVD64_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149987.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            2182..2490
                     /locus_tag="IVD64_002786"
     CDS             2182..2490
                     /locus_tag="IVD64_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149988.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            2494..2856
                     /locus_tag="IVD64_002787"
     CDS             2494..2856
                     /locus_tag="IVD64_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149989.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNEYDGLLGVIPSKK
                     SQMNESKFMSIFNDVNLR"
     gene            3078..>4015
                     /locus_tag="IVD64_002788"
     CDS             3078..>4015
                     /locus_tag="IVD64_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6149990.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELGYISRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKD"
BASE COUNT         1392 a          564 c          924 g         1135 t
ORIGIN      
        1 gcatttcaaa aatcgatcac tcgtttgtct atccgttttg gtcttgcaga aaaggatatg
       61 gggctttctg aggaattaga agtattagag caggagaaaa ccttatttta tcgtcaaaac
      121 gtctcacagc ataaattaga gcgttctgaa gatgagatcg agtggtagtt acgatgtgca
      181 gtcaacggta ccggaggcgg tctgtgccga ggataaggag ttgacggcta aaccactagc
      241 ctattaagct gacatttgct aaaaaatggc aagtccattt tttggccaac ctagcgttag
      301 cgaaagtttg ggggcgccct tttcaaaggg agcggtttta tttttgcatg actaagaatg
      361 agtgggaaaa acttaaccgc atttctaaca ggcggttaaa gtatcaaagt ttgacccttc
      421 ccttaaaaat gcagtggtca attgaccgaa ttttatgcag aagtgggttt ttgagttttg
      481 gggtttgaaa cgagaaaaaa gaggtcgatt tcgacctctt tttttgatgt ttcttcttat
      541 cttgatacta taggcaacta tttctgcgaa gaaatcgggg ctatagtatg ggtctatccc
      601 ttgtggctct aaggaagaac aaaaaaaaag attgcttttt cttccctagt attttatagt
      661 tttaattgac tagaaaaaaa ctaaactaga aaggagagca atctattttt tgttatcggc
      721 ttttcagcct tttttgtata caaaatttgt agattcagta tagcaagatg aaaaagaaaa
      781 agcaagattt gattaacgga acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg
      841 catttagtga taatcgaatt tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat
      901 atttagaatt tattgcaacg attgataagg agaaaaagaa attggtccaa gctcactttt
      961 gtaaaaatcg tttctgtccg ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt
     1021 tgtcgattat gatgcaagct attgcggaag aaaagcagta tcaatttatt tttatgacgt
     1081 tgacgacacc gaatgtaaaa gcggaatgtt tgaacgatga aattgatttg tttaatcatg
     1141 cattgaaaaa gatgtttatg cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa
     1201 aattggaaat cacgtacaac aaaaaacgga atgactataa cccgcatttt cacttgattt
     1261 tggcagtaaa taaatcttat ttcacggata aagattatta tatcaagcaa tcggaatggt
     1321 tagatttgtg gcgggaagtg accggaaaaa cgggaattaa tccggatggt accgacgaaa
     1381 ttacccagtt ggatattcgc aaggtcaaag ggttccaaca agaaaaagca gtacaggaag
     1441 ttgcgaaata ttcggcaaaa gattttgaac tgaccgaaag tcaggaagtg tttgacacat
     1501 tctatcatgc aatgaaaggt cgtcagttga ttacgtttaa cggtgtattc aaagaataca
     1561 agaaaaaata tgagtcgggt gaccttgatc gttataagaa aaaagatgag aatgaatatt
     1621 tttggttttt gactgcttcg tggatgagga aagaatcgaa atatgcggtt gattatcgtg
     1681 agatgagtgc tgatgaaaga aattattttt caggaatgta tgtcgatgaa aaatctgttg
     1741 agggggaaaa tgaatgactg attcaacgga taaaatgaca attaaggcgc ttgctgatga
     1801 actaggaata accaaaaata cagtgagaaa ttatctagtc aaagccggat atgatcttgc
     1861 tcggtacaag aaaaatggaa ttatcttgct agacaaagag ttgattggtg ttgctcggtc
     1921 aatttatcgg tcaaaaggtg gtcaattgac tggtcattta ccggtcaata gcacgggcag
     1981 tgattttcct ttggaaaaag agaatgagtt tttgcgtgat caattagctg agaaggacaa
     2041 acatatttct gaattaacag aaatgattcg tgatcaacaa aaattgaccc tgcttcagca
     2101 acaaaaaaac gatcagttgt tactagaaaa tcaagaatta aaaaatcgaa aatggtggca
     2161 attctggaag tgagatgtga catggataaa attgatttga gagaagaaac agtcaataga
     2221 attgaagtga tgtatgaacg gttagctgat tatttgaatc tttcggatga agataattat
     2281 agtgagatgc ttgaacgaat gatggatgtt ctcaactcgg ttatcaatgc tgatctagca
     2341 aaatcttcag ttttgactaa tgaatttgtt cataagcatt tagcgcaaac aactgatgaa
     2401 gctgaaaaag aattttggtt gaaattaatg agtcatatta tgcagttaaa ccaaatttcc
     2461 gttattaatt ccaaggaaaa ggggatgtag tttatgggga tgactttagc agatattaaa
     2521 gtgcaagaag aaaaattaaa aaaagaacag gcgaaacttc gccaacagaa gaaacgattg
     2581 attgagaaag aaaatgctgc aatgggcaag gctcttcgca agcgttttgg gtttgaatct
     2641 tttgctgatt ttgagcggtg gttatcgtca atttctgacg atcagcaaca cgaaaaatca
     2701 gtggataatg agtggcttgc tgatgaaaaa tcagcgccaa gcgaacgaga ttatcccaat
     2761 gaatatgacg gcttactcgg agtgataccg agtaaaaaaa gtcagatgaa tgagagtaaa
     2821 tttatgtcaa tttttaatga cgtgaattta cgataagcta ttattttgcg tgaagcaaaa
     2881 taaagcgtaa aaggagttga gagcgtgaca cgctttcggc tccttttttc gttgtcactt
     2941 gtgacagcga aatccctcgg agagcgtttg gaattgtgac agtcatgtca caattaggaa
     3001 aacgttactc tttgacaaag agtaacagaa aggtttacac tctttttatt agcgacagcg
     3061 cgaaggaggg gtctaacttg gggtttagta tttcgttcaa aaagggaaca aagaatacaa
     3121 gcatttctca taataatcgt gagttaactg atagccaaaa aatcaatgac tggcataagc
     3181 atattgattt cacaaaatcg gatgaaaata tctatttgga acaaacaaat attcgtgaaa
     3241 aatatgaaga attattcggt gaagccgtag aaaaatataa tgcaaagcag aaaagagccg
     3301 atagaaaaat cgaggactat ttagcaaagg ttcgcaagga taaaaaattg gaaccacagc
     3361 gagaatttat cgtgcaaatt ggtactcttg atgattttcg tactactcgt gatgatggtt
     3421 cttctactgg catatcggaa caacaagcag aacaaaatcg agtgattgcc aacaaaattt
     3481 tagttgagta tttcaaagaa tttaaagaac ggaatcctag tttatccgtc tacaatgctg
     3541 ttattcataa tgacgaagta agtccgcacc tgcatttgaa tattgttcct gttgctgaag
     3601 ggtataaacg gggtgttcaa aagcaaccaa gttttaacaa agccttgcaa cagcagggtt
     3661 tacgagcaga taaagagaat ggtcgagtga tttggaataa ttttaggaat caggaagttg
     3721 agtcaattga acgtttgatg gcagattttg gttgggagcg tgagttagtc ggtactaaca
     3781 aaatcaaaga tgttcacgaa tataaagaaa tcatgagcga gatttcagaa ttgcgtcaaa
     3841 cggccgtaaa agagcgtgag agcgtttctg atgagttggg gtatatttct aggcaaagag
     3901 aagaaaacga gcgagaaaag gctctggtgg cttctgagag agcaaagatt gaagaagaga
     3961 agaaaaatat gctgaatgta aaaaaagaga tggcggtcat tttccaaaaa gatat
//
LOCUS       DACBZN010000171         3923 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.174, whole genome shotgun sequence.
ACCESSION   DACBZN010000171 DACBZN010000000
VERSION     DACBZN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.174"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(573..1130)
                     /locus_tag="IVD64_002789"
     CDS             complement(573..1130)
                     /locus_tag="IVD64_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149991.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IVD64_002790"
     CDS             complement(1309..1920)
                     /locus_tag="IVD64_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149992.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSARYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IVD64_002791"
     CDS             complement(2033..2290)
                     /locus_tag="IVD64_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149993.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IVD64_002792"
     CDS             complement(2887..3165)
                     /locus_tag="IVD64_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149994.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IVD64_002793"
     CDS             complement(3199..3450)
                     /locus_tag="IVD64_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149995.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          800 c          520 g         1409 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atatcgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACBZN010000172         3891 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.175, whole genome shotgun sequence.
ACCESSION   DACBZN010000172 DACBZN010000000
VERSION     DACBZN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.175"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            119..712
                     /locus_tag="IVD64_002794"
     CDS             119..712
                     /locus_tag="IVD64_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6149996.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            728..1627
                     /locus_tag="IVD64_002795"
     CDS             728..1627
                     /locus_tag="IVD64_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6149997.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKKLYNQEMPQLMKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            1630..2115
                     /gene="ssb"
                     /locus_tag="IVD64_002796"
     CDS             1630..2115
                     /gene="ssb"
                     /locus_tag="IVD64_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6149998.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2137..2394
                     /locus_tag="IVD64_002797"
     CDS             2137..2394
                     /locus_tag="IVD64_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6149999.1"
                     /translation="MAKKNSLTMDLLIPVIAFIDGISLGITENLLVNRIAKGSLFLVF
                     VYLVCSYLLKRVGKDNSFWRLTKVTIGATAVILTAILPLYM"
     gene            complement(2554..2676)
                     /locus_tag="IVD64_002798"
     CDS             complement(2554..2676)
                     /locus_tag="IVD64_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP6150000.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            2830..3090
                     /locus_tag="IVD64_002799"
     CDS             2830..3090
                     /locus_tag="IVD64_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150001.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLHTSHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3533..3733
                     /locus_tag="IVD64_002800"
     CDS             3533..3733
                     /locus_tag="IVD64_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6150002.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PICGKKYTLSYGDYILIEEKGR"
BASE COUNT         1397 a          658 c          762 g         1074 t
ORIGIN      
        1 aagaaccagc aacggattca aaaagaaatt gaattagatc gataaatatc tttcttgtta
       61 atataaaact aacgtgatat agttatgtta gttttatatt aactttaggg gaaaagcaat
      121 gaaagacaaa atccagtgca tttttgataa acataatggg aatctatcct tgaaggatgc
      181 tagaagtgaa ggcttatcgc ctatgacgtt agatcgatta gccgaaaagg acaaaataga
      241 aaaggtagca ccaggtttct ataccttaaa agaagcgttc gtggatgaat tgtatctcgc
      301 tcaaagtatc tattccagag gtattttttc tcatgagact gcgctcgatc tttttcaagt
      361 cggaacgtat atccctaaaa agatcaatat gatgtttcct aaaggataca atgtttcaaa
      421 ggagaaatta gacgagtatg ctattcaacc gcattacacg aataaagaaa attttgacct
      481 gggaatcaca gaaacagaaa gcttttacgg caataaaatt ttagtctatg atttagagcg
      541 aacgttatgt gatatgtgga atccaagata caaagcaagc gtagaggtaa aacaagaagc
      601 attaaaagaa tatatgacaa gtaccagtag aaatacaaat aagctaagaa gatacatgga
      661 tattttaaaa tcaccaaaag aaatgacttt gtatatgttg ccactttatt gagaggagta
      721 aaggtgaatg acaccgacac aatttaaagc aaaaacgaga aatttcgcaa cagagaaaca
      781 tttaaatcca caaattgtcc aaagatatta tatgatggaa aaattattag aacgtattgt
      841 tgaatcgcct tatcatgatg attttgtatt aaaaggggga tttctgatcg gatcaaaata
      901 cgggttagaa aaccgttcaa ctgttgatat tgatacgact tatcgaaatt cgaaactaac
      961 aaaagaaaag ttagaaactg ttttttctga gttaacagct gatcccacaa aagatggaat
     1021 tcgatttgaa ttaaagggat taacagagac gagggaagcc gattattacc caggttttca
     1081 agcaaagttt attgcttatc ttgagaatgc acgtgtacca tttaaattag atattacttc
     1141 aggtgattct attattccag cggcattgat gtatgaacac aaattaatgt ttgaagataa
     1201 aacgattcat attccagctt atcctacgga acaaattcta gctgaaaaat tacatgctac
     1261 gtttagtttc ggaaaagata attcgcgcat gaaagacttt tatgacctgt atatgattcc
     1321 aaaactagaa cgtatcgatg agaaaaactt gtataagagt gttcaaggaa cgttcaagga
     1381 aagaaataat caatctgtgt ttaaaaaatt gtataatcag gaaatgcctc aattgatgaa
     1441 ggatgagggg ttgagggaaa agtggaaaag ataccaacaa gaaaattatt ttgccaaaga
     1501 catttctttc gaatctacac taacttccgt cgattctttg atgcaatcaa ttgtagagca
     1561 agagaaagta gaacgaaaac aaaattttaa gagatcacaa caaatgaatc aagaaatgga
     1621 gagataaaaa tgattaataa tgtaacttta gtaggacgat taaccaaaga tccagattta
     1681 cgttatactg caagtggtac tgcagtagcg acttttaccc tagccgttaa ccgaaatttc
     1741 acgaatcaaa acggcaatcg agaagctgat tttatcaatt gcgtgatttg gcgcaaacca
     1801 gcagaaacga tggcgacatt agctaaaaaa ggcagcttga tcggtgttgt cggacggatt
     1861 caaacacgga cttatgacaa ccagcaagga caacgtgtgt atgtgactga agttgtagca
     1921 gataactttc aactattaga aagtaaagca gcgacagaga gccgtgcgca tgcagatcaa
     1981 tcgtccacca gtccaagcac cgccaccttt gaacaacggg atacagcgac gcctaacaac
     2041 aatggattga acgcttcaca gaatccattt ggtggacaat caatcgatat tagcgatgat
     2101 gatctaccgt tttagttcga gaaagaagga gtaagcatgg caaaaaaaaa tagtctgacg
     2161 atggatctat tgatacccgt aattgcattt atagacggga tttctttggg tatcactgaa
     2221 aatcttttgg taaatagaat tgcgaaagga tcactatttc ttgtattcgt gtatcttgtc
     2281 tgttcttatc ttctaaaaag agtaggaaag gataattcct tttggcgatt gactaaagtc
     2341 acaataggcg cgacagctgt tatacttacg gctattctac cgctttatat gtagggttaa
     2401 cgggaaatca ttgaacttct tgcgaaatat tgttataatg aacatgaaaa agaggagttg
     2461 cgcaaacaac ccctcattaa cagcaccgtt taaagaacgg cggctcagat taattattta
     2521 ttgagaaata accgatcctc gctaaagtga cggttatttc tttttgtcat ttttaagcat
     2581 atttacaatc aaactaatca aagcaatggt aaacatacca aaaccaagaa tggtttgaat
     2641 tgtctcatat gcggacaaaa ggcttactcc tttcatagat tttagcactt acattcataa
     2701 gcaccaccac cttccagaag tcgccaccgt cattaaactt tactgtcttg atcattatag
     2761 catatctaaa atcgaataaa aattctcaat tttagctgtt tttgctataa ttttagcgag
     2821 gtgatcgcga tggaacatta tactggttta ctcgataaaa ttcgggtgat caagacacag
     2881 ccattgcttg tgcgatttac attacacacg agtcatgaac caatcaattg tgtagtggcc
     2941 aatatagaaa tagcggataa attgctgatt ttggaagaag gcaagtatac tatttcagtg
     3001 acaggtcact tcaataaacg gaatcaatta gtcattgaat cgatgcaaat tagaaacccc
     3061 gatcatttta cgcgatcgat gggcatataa aagaagagct gcaatcctaa tgtaggactg
     3121 cagctctttt tgttttacat agaaacggga acaggaaaag gcctttttag gaaggcacaa
     3181 gtgccttctc aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa
     3241 aggaagtgaa cgctgcacgt tcactaagat aaggggtctt gcaaagcaag acaaaataga
     3301 aaaaacttcc tctcaccaaa aggttcgttc ctgttcgtta tctctttatt gatttgccag
     3361 accggcaaca agttgccttt tatcaccaaa aagcgacaag gacaagggta aatggcatgc
     3421 tgcgcgccct tgtccttgtc gcttttcgtt gattcattct ggccaacaaa tcaataaaga
     3481 aagaactaac gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa acatgcgaaa
     3541 gaaatttgat tattggggtg ttcccttcag cgaattattc ccaaactatc atgcaccaca
     3601 tactgtcgaa tgcgattgtg gagaaagagc gaaatgtatc aaaagctatc gtttgtatca
     3661 atgtccaata tgcgggaaaa aatatacctt gtcttacggc gattacattt tgattgaaga
     3721 gaaaggaaga taaaatgatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg
     3781 ataaaatggc acaaaaaatg gaggatatga cgttcctata caacgaaaca cgtgttccaa
     3841 aaaaacatta taaagaaaaa ttgtcggtag ctgtggaaga aatgattgaa t
//
LOCUS       DACBZN010000173         3864 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.176, whole genome shotgun sequence.
ACCESSION   DACBZN010000173 DACBZN010000000
VERSION     DACBZN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.176"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(247..468)
                     /locus_tag="IVD64_002801"
     CDS             complement(247..468)
                     /locus_tag="IVD64_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150003.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(566..811)
                     /locus_tag="IVD64_002802"
     CDS             complement(566..811)
                     /locus_tag="IVD64_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150004.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1051..1665
                     /locus_tag="IVD64_002803"
                     /pseudo
     CDS             1051..1665
                     /locus_tag="IVD64_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2035..3036)
                     /locus_tag="IVD64_002804"
     CDS             complement(2035..3036)
                     /locus_tag="IVD64_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP6150005.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3195..3461)
                     /locus_tag="IVD64_002805"
     CDS             complement(3195..3461)
                     /locus_tag="IVD64_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6150006.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3451..3807)
                     /locus_tag="IVD64_002806"
     CDS             complement(3451..3807)
                     /locus_tag="IVD64_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6150007.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1432 t
ORIGIN      
        1 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
       61 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      121 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      181 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      241 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      301 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      361 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      421 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      481 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
      541 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
      601 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
      661 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
      721 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
      781 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
      841 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
      901 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
      961 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     1021 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     1081 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     1141 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     1201 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     1261 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     1321 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     1381 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     1441 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     1501 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     1561 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     1621 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     1681 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     1741 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     1801 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
     1861 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     1921 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
     1981 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
     2041 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
     2101 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
     2161 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
     2221 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
     2281 actccttaaa ttgttatcac tttcccattg aactactttt ctataagttg aattatcagt
     2341 tgaaaagata gaattttcat ccataaacgc atatattagc agcaatcttt catcattgtc
     2401 aaattctatt ggatcaaaaa tatctaagtt gtcatcaaaa tctttatcag ttccggtaac
     2461 ttttttgtta attactgaag gtgtcttgta agaaatagaa tctaaatcaa agcgttttga
     2521 ttctaaatta ttttgtgctt tctcaattaa ataattcact ttttttgtga gaaaccttct
     2581 gatattgtct ttgctatatg ttccataaaa acccttatct tctaaagagt ccctgtactt
     2641 cttaacttct atatactctt cagccaaaac ttgtggaact ggttcatcta agaaaaaaac
     2701 ccctacctcg cctttgctat ttttataaaa gtaatcaatt tctgctatag ttcccgatct
     2761 ataattatca actggagaac ccaacttatg gccaaaaatc gctaataata tatcactatt
     2821 ttctacaatt tgtttattaa taacactttg cccagagcta ttatttatat aagtaggaat
     2881 cgcattcgtt tcccatcgaa ttggcatcaa aattactttt gtattatctg tattttctat
     2941 attccatcta tatatggttt cttctacttc attcctcttt gctgctatat cacctggtga
     3001 tgctataaat acttttatta gatttatggt tttcatttat tgtacctttc tctttatgtc
     3061 tctttaaagt aatttttgta taaaatttat tgacttttac actataggac aacttttatc
     3121 tatttagtac aaaaaggccc actaaactaa ttgattagtt aggagcctat tacaggtata
     3181 ttttcaactc gcttttaagc aaattctact accagctttt tgcctaatgc cctagcgata
     3241 tcatcaagtt tatcgaaaga tacgttatcc cctctctcaa ttcgagcaat agtagattga
     3301 gttgtatgtg ctttttgtgc aagctccgct tgagttaggc ctgcttcctc ccgtgcatga
     3361 taaagagcaa ccgcagtttc gagttttctt ccttcacggt catatgcttc tgcaaaatct
     3421 ttgtcttttt gcttttcttc aatcaatttt ttaatcatca tttgtgaacc actcctctct
     3481 aagttctttt gcatgtttaa gttcacttat tggtgttttc tccgtttttt tagtaaatcc
     3541 atgggtaatg acataccttc cattgaccac atggaaatag atagctcttt ggatgtttga
     3601 tcctacctta cttcttaatt cgtacaaatt agtgtctaat tttttcaccc atttcaaccg
     3661 ttgagctact agcatcccat tcttttctgt ttcttctatt gtacgtaaaa gcttcgcact
     3721 atcttttttt ggtaatgttt ccaaccattc taaaaattca tcatgaccat taggtctttt
     3781 aaatgtttca aaagatattt tttccatacc caattatatc atatattgat tttatattat
     3841 agtatataac ataaaaattc gaat
//
LOCUS       DACBZN010000174         3735 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.177, whole genome shotgun sequence.
ACCESSION   DACBZN010000174 DACBZN010000000
VERSION     DACBZN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.177"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..478
                     /locus_tag="IVD64_002807"
     CDS             <1..478
                     /locus_tag="IVD64_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6150008.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="IVD64_002808"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="IVD64_002808"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP6150009.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="IVD64_002809"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="IVD64_002809"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP6150010.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..3697
                     /locus_tag="IVD64_002810"
     CDS             3200..3697
                     /locus_tag="IVD64_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP6150011.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1230 a          596 c          807 g         1102 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
     3661 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
     3721 aagagaaatc aagaa
//
LOCUS       DACBZN010000175         3680 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.178, whole genome shotgun sequence.
ACCESSION   DACBZN010000175 DACBZN010000000
VERSION     DACBZN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.178"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            418..1032
                     /locus_tag="IVD64_002811"
     CDS             418..1032
                     /locus_tag="IVD64_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6150012.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1805..3544
                     /gene="yfcC"
                     /locus_tag="IVD64_002812"
     CDS             1805..3544
                     /gene="yfcC"
                     /locus_tag="IVD64_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP6150013.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         1156 a          598 c          754 g         1172 t
ORIGIN      
        1 tttttttgta ttttcttgct tgcacttgga taagagatta cttacaataa attcagatag
       61 aaacagctga ataaaaaaag gctccctgac ccttagttgg cgctaagaat cagggaatga
      121 ccagtcacac acatacaccc tgaccgatca ttttgccata gtcaatgcag tgcatcgacc
      181 taatttattg tacctaatct agggtgaaaa ttctacccct atgtagatat tttccatggt
      241 ctgcacgttg ttttatttgc tatgtcaagg tattgggcgt attcgttcaa tgcctttttt
      301 gtgtttgaaa cagcaaagac cacatgtgtt actgtctaac tactgttttt ttatgtactt
      361 ccttagatta ggcagctgtt tctattcatg tcaagtatag ctaggcggga gagataaatg
      421 agtttatgtt attttgacta ttatcaccaa ttggaagaga aattaaggat agaagagccg
      481 ctgatgcttc aagctggcac ttggatcgcg aaacaaatta atcaaggtgg tagattgcag
      541 atatatgcta gccgcacact aagtggtgta gcatttgaat tttgggatca aaccccggat
      601 cttgtaccaa gtatattgat tgaaaatcca gccggaggtg tctatgaaac gctagaaggt
      661 gcaggacagg caattattga tcagctttct gtcaaatcag aagacatttt tcttcttctt
      721 tcgaatgaag gaagaaaccc ggcaatcatc gaactagctc agtggatcaa agaaaatggt
      781 catccattaa tcattgttac tggatttgat ttgtcacgaa gtatcaaatc gcaacatagc
      841 agtggtttgc gattatttga atttgctgat ttggttttgg acaaccacgc caatttacat
      901 gatgcagtct tgactttgcc aaatctagat ccagcgatct gcggaacagc ctctcttgca
      961 acgatctttt tgctgcagca agttttgtat tttacagcaa aacagctagt ccaggacaca
     1021 agtggaaaat agttatccac atgttgacaa agttccttaa aaaaataatg ttccacataa
     1081 aaatggagcg tttgaaaaaa agaaaacgat tattaatgag gattaacaac gactactttt
     1141 tttgttttag ttattttaag atggatcatc tttgatttta agcttgtttt gtttaaagtt
     1201 tgttattttt ataagttgtt tcacagttat gagaaatgag ttataagaat atgtagtgtc
     1261 agaaaaataa aaagtagtgg aaaaagagac aagaaagaat ccatatggat tctttcttgt
     1321 ctcttaatac acgttgatga aagagtcttc cccaaaactc tttacaaagt aattatagta
     1381 catttgttat cattattgca atgaagttta cctgatataa caaaattgtc caacaggata
     1441 cctttttcta atagaaataa aatacacaaa aattgaagga tagataatgg atatttataa
     1501 gtgtttatta ttttttttgc aatgaaattc aatttttcaa tgattgtaaa atgaaaaaaa
     1561 caggacgaga ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa aacagagaaa
     1621 taaaatactt aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca
     1681 aaatagaaaa aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat
     1741 aattcatttg attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat
     1801 aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac
     1861 accccatacg tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt
     1921 cgttccagca ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac
     1981 aagtactcgt actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc
     2041 gtatgtgttt gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga
     2101 tgttccggaa aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga
     2161 aaaactagat gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat
     2221 ctatgatact tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg
     2281 cggctttggt ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc
     2341 tgctgtaggg atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg
     2401 taccggagcg atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg
     2461 tttagcttta ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc
     2521 agaagaggta attccattct caatggtgat ggttccattt gtcattgcgt taggatatga
     2581 ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg
     2641 gatgagcccg tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg
     2701 tgcgacattc cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat
     2761 gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga
     2821 ttatttccgt tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca
     2881 taaattgatc ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca
     2941 aaaaggctat tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg
     3001 aatcattgct gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt
     3061 ccaatctggt gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat
     3121 tcttcttgtc ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca
     3181 tgggatcggg acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat
     3241 cttccaaagt ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat
     3301 gccaatcatg gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc
     3361 ttatcagctt ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg
     3421 tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca
     3481 aggattcctg tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca
     3541 gtagtactgg agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa
     3601 ccgtcatttt acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag
     3661 atcctatcat cagttatact
//
LOCUS       DACBZN010000176         3493 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.179, whole genome shotgun sequence.
ACCESSION   DACBZN010000176 DACBZN010000000
VERSION     DACBZN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.179"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            137..748
                     /locus_tag="IVD64_002813"
     CDS             137..748
                     /locus_tag="IVD64_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6150014.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IVD64_002814"
     CDS             821..1750
                     /locus_tag="IVD64_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP6150015.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IVD64_002815"
     CDS             1908..2645
                     /locus_tag="IVD64_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP6150016.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..>3493)
                     /locus_tag="IVD64_002816"
     CDS             complement(2693..>3493)
                     /locus_tag="IVD64_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6150017.1"
                     /translation="HHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLK
                     RKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITD
                     GLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAAL
                     EARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYS
                     HRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1154 a          540 c          673 g         1126 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atg
//
LOCUS       DACBZN010000177         3451 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.180, whole genome shotgun sequence.
ACCESSION   DACBZN010000177 DACBZN010000000
VERSION     DACBZN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.180"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IVD64_002817"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IVD64_002817"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP6150018.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IVD64_002818"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IVD64_002818"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6150019.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IVD64_002819"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IVD64_002819"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6150020.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IVD64_002820"
     CDS             complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IVD64_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP6150021.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT          982 a          755 c          594 g         1120 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg a
//
LOCUS       DACBZN010000178         3438 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.181, whole genome shotgun sequence.
ACCESSION   DACBZN010000178 DACBZN010000000
VERSION     DACBZN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.181"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            283..1386
                     /locus_tag="IVD64_002821"
     CDS             283..1386
                     /locus_tag="IVD64_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6150022.1"
                     /translation="MTEKGRSYLKEQRKFPEWLIDYGEQQGLILEMKPRNERKNYIVQ
                     DDRLDYAIVFPWKDRHTDQILGATYQGTIVDYERFGKRGVDKRIDINPTPNHGFNVLL
                     GSPKNLKFFESPIDLLSYAALNRETIKDTWLVSMDGLKDAVVGHYVREAVTELAPKEE
                     FPESIEICVDNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNLPNDWQVPREYQTIYEE
                     VGKEMRVAPEAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKLAPKEKAQSIVVKEKCI
                     EAAQQLKSCEKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYFEGYKNHEHEFVSEVKK
                     DWNDQLKHEIQQQAIRKQKRAMLFQQGRQQERE"
     gene            1397..>3438
                     /gene="topB"
                     /locus_tag="IVD64_002822"
     CDS             1397..>3438
                     /gene="topB"
                     /locus_tag="IVD64_002822"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6150023.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDEKWKQWSLAHLPIFPNQYHYHVPKDKKKQFNVVKRQLQSADT
                     VVIATDSDREGELIAWSIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAHVITQNGAFDAKLDPNETQATQEAFEAYLKDKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEEKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPVDLGWKKLFGK
                     ESDGKEKEEEPLLPKVAKGEMVTVDLQVLEKETKPPKPYTEGTLITAMKTAGKTVDSE
                     KAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKE"
BASE COUNT         1221 a          616 c          772 g          829 t
ORIGIN      
        1 ttagcggtag tcaataaagg acgagactat cgtttagaag accatgattc ttttgttttc
       61 gatcgaagaa aacagcgatt ttattggaac agtcaaggta ttggtgggga tattattgaa
      121 ttgactcaat tgttttttat tgacgattct attcaaaacc caaagcaacg ttttaaagca
      181 gcgctagatt atattataaa ggacgaaaat aaggtggaac gggtggaaaa tcttcacttt
      241 gaaacacaag aatataagca ccacccaatt gattatcgac cattgacaga aaaaggacga
      301 agttacttaa aagaacaacg aaaattccct gaatggttga ttgattacgg agaacaacaa
      361 gggttgatcc tggaaatgaa gccacgcaat gaacgaaaaa attatattgt gcaagatgat
      421 cgcttagact atgcgatcgt ttttccctgg aaagaccgtc atacagacca aatattaggg
      481 gcaacttatc aagggactat cgtagactat gaacgttttg gaaaacgggg tgttgacaaa
      541 cgaatagata taaatcctac gcctaatcac gggtttaatg ttcttcttgg atctcctaag
      601 aatctgaaat tttttgagag tcccattgat ttacttagtt acgctgcttt aaatcgcgag
      661 acgatcaaag acacgtggct tgtttctatg gatggactga aggatgcagt agtggggcac
      721 tatgttcgag aagcggtgac agaattggcg ccgaaagaag aatttccaga atcgattgag
      781 atttgtgtgg ataatgatcg agctggacac gtattctggg ataaggtaca ccgacttggt
      841 gtgtcgaatc catttacagg aaaagcggtg tattttgaac cgaatcttcc caacgattgg
      901 caagtaccga gagaatatca aacaatctat gaagaagtag ggaaagaaat gagagttgcg
      961 cctgaagcaa tcatggctat tcataaaatc gaaaacaacc taacggatac taaccaaatc
     1021 gtatcgttcg gtgaaatcca gggagtattt gccaaaaaac ttgcgccaaa agagaaagcc
     1081 caatctattg ttgtaaagga aaaatgtata gaagcagctc agcagctaaa atcgtgtgaa
     1141 aaatctgatg gaacatacga ttttgatcgt ttttatcgtg gaaaagggaa aattaatgaa
     1201 caaattcaat tttcattgaa ggccaaacac tattttgaag gttataaaaa tcatgaacac
     1261 gaatttgttt ctgaagtgaa aaaagattgg aatgatcaat tgaagcatga gatccagcaa
     1321 caagcaatca gaaagcaaaa acgtgcgatg ttgtttcaac aaggcagaca acaagaaagg
     1381 gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aaaccgtcac aagcgaaggc
     1441 ttatgccgat agcttttcta aagctacccg aaaagacggg tattttgaaa tccaagatcg
     1501 gttgtttcca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     1561 gcccgatatg tacgatgaaa aatggaaaca atggtcatta gcgcatttgc ctatttttcc
     1621 aaatcaatac cactatcacg ttcctaaaga taagaaaaag cagtttaatg tcgtcaaacg
     1681 ccagctgcag tcagctgata cagttgtgat cgcgactgat agcgatcgtg aaggggagct
     1741 gattgcctgg tcaatcattc aacaagcagg cgccgatcaa ggaaaaacct ttaaaagact
     1801 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt tacgagacgc
     1861 agaagagacc tatccaaaat ttgaagaagc acaagcacgt cagatcgccg attggttaat
     1921 tgggatgaat ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     1981 tttttcctta ggcagagtcc aaaccccaac gctctacatg atttatcaat tgcaggaaaa
     2041 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gctcatgtaa tcactcaaaa
     2101 tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag aagcatttga
     2161 agcttattta aaagacaaag gtgttcaatt aggaaaacag ccaggtacga tccttcaagt
     2221 cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga gccttcagtc
     2281 taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca tgcaagggtt
     2341 gtatgaagag aaatatctaa gctatccgcg aacggatacc ccttatatta cggaagggga
     2401 atatgcgtat ctgttggatc acttagacga atacaagcat tttttgaggg ctgaagaaat
     2461 tccaacccct attcatacgc cgaatagtcg gtatgtgaac aataagaaag tccaggaaca
     2521 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aattaagtcc
     2581 actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     2641 gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt taaaagcaat
     2701 cggaaaagtg ccggtagatc taggttggaa gaagttattt ggaaaagaga gcgacggaaa
     2761 agagaaggaa gaagagccgc tacttcctaa agtggcaaaa ggagaaatgg tgacggtaga
     2821 tctacaagtt cttgaaaaag aaacaaagcc accaaaacca tataccgaag gtacgttgat
     2881 cacggcgatg aaaacagctg ggaaaaccgt ggatagtgaa aaagcgcaat ccatattgaa
     2941 agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca
     3001 aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct
     3061 ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg ccaaatggga
     3121 aagctacttg ttaaagattg gagaacgaaa aggaacacaa accacttttc tagccaatat
     3181 ccaaaaattt gtctctcact tattggaagt cgtcccagga caaatccaat ccactgattt
     3241 tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt
     3301 aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcttg
     3361 tacgtctgaa gcctgtgatt ttaaattatg gacaacgatt gccaaaaaga aactcacagc
     3421 gacacagctg aaggagat
//
LOCUS       DACBZN010000179         3402 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.182, whole genome shotgun sequence.
ACCESSION   DACBZN010000179 DACBZN010000000
VERSION     DACBZN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.182"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(75..1073)
                     /locus_tag="IVD64_002823"
     CDS             complement(75..1073)
                     /locus_tag="IVD64_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6150024.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="IVD64_002824"
     CDS             complement(1148..1444)
                     /locus_tag="IVD64_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6150025.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="IVD64_002825"
     CDS             complement(1437..1730)
                     /locus_tag="IVD64_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150026.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="IVD64_002826"
     CDS             complement(1876..2427)
                     /locus_tag="IVD64_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP6150027.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="IVD64_002827"
     CDS             complement(2601..2900)
                     /locus_tag="IVD64_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP6150028.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3235)
                     /locus_tag="IVD64_002828"
     CDS             complement(2927..3235)
                     /locus_tag="IVD64_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6150029.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
BASE COUNT          990 a          664 c          464 g         1284 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttt aggaagtgta cctatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaagttgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttgtcc tttattttga aacatttctt
     3241 ttccctcctt tgctataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa at
//
LOCUS       DACBZN010000180         3398 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.183, whole genome shotgun sequence.
ACCESSION   DACBZN010000180 DACBZN010000000
VERSION     DACBZN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.183"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..276
                     /locus_tag="IVD64_002829"
     CDS             <1..276
                     /locus_tag="IVD64_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP6150030.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IVD64_002830"
     CDS             419..640
                     /locus_tag="IVD64_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6150031.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IVD64_002831"
     CDS             656..1498
                     /locus_tag="IVD64_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150032.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IVD64_002832"
     CDS             1561..3363
                     /locus_tag="IVD64_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP6150033.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1229 a          469 c          702 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DACBZN010000181         3368 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.185, whole genome shotgun sequence.
ACCESSION   DACBZN010000181 DACBZN010000000
VERSION     DACBZN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.185"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..135
                     /locus_tag="IVD64_002833"
     CDS             <1..135
                     /locus_tag="IVD64_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6150034.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            113..1588
                     /locus_tag="IVD64_002834"
     CDS             113..1588
                     /locus_tag="IVD64_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP6150035.1"
                     /translation="MESFVSLNLTNNTSKKNQPKDIKDIVDTRMYKERDDLNYTLIIN
                     KDGNYSWRPLVLLHPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASK
                     GSKRTDLGETILNWWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEK
                     VKNDENKCSGSLGDKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELK
                     KLENLKDYKVLRYRDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIII
                     DSIKKDKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKK
                     ALSEFNKERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAR
                     EIVEMIINKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVD
                     WLNEDAKKSFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            1926..>3368
                     /locus_tag="IVD64_002835"
     CDS             1926..>3368
                     /locus_tag="IVD64_002835"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6150036.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1238 a          536 c          601 g          993 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatac ttcaaaaaaa aaccaaccga aagatataaa
      181 agatattgta gatacacgaa tgtataaaga acgtgatgac ttaaactata cgcttattat
      241 caataaagat ggaaactatt catggcgtcc tctcgttcta ctacatccta ttctatatgt
      301 agatttggtt aattgtctca ctaaagaaga ccattgggag gagttaaaaa agaggtttag
      361 tgaatttaaa aaagataaaa atattaactg ttattctata cctgttgaag cttccaaagg
      421 ttcaaaaagg acagatcttg gtgaaacaat actaaactgg tgggaatatt ttgaacaaaa
      481 aagcttagct cagaatatta aataccaata ttgcatgttt actgacatcg ctgattgtta
      541 tccatcaatt tatactcact ctataacatg ggccatgtat ggtaaagaaa aagttaaaaa
      601 tgatgaaaat aagtgttcgg gctcacttgg cgataaaata gataaaaaaa tatctggaat
      661 gcaacacaat caaacaaatg gtatccctca gggttccgtt cttatggatt ttgtagcaga
      721 gatcgttcta gggtatgttg atttgagatt atcgaaagaa ttaaaaaaat tagaaaattt
      781 aaaagattac aaagtactca gatatagaga tgattataga atattttcaa ataatcgtaa
      841 tgatttggaa caaattatga aagtgttatc agaagtctta tatgatttaa attttaagtt
      901 aaattctagt aaaacaaaat tatcagatga tattatcata gattctatca aaaaagataa
      961 gctctattgg gaagaaatta aggcaagtat aagaatgaat gccacaaact tagatggtga
     1021 tagagaaatt cgttatagta taaatttaca aaaacattta ttggaaataa aaaagttagc
     1081 agataagcat cctaattcag gccaattgaa aaaagcactt tctgagttta ataaagaacg
     1141 cattattaaa atcacttcaa agccgaacga tcttttacca cttattggta tcctggtaga
     1201 tatcatgata aataacccgg gttctttgcc gcagtgtgtc ttaattatag ggcagttatt
     1261 aaatttcgaa aatgagagta aggctagaga aatagttgaa atgattataa acaaatatgc
     1321 tttaaagcca aatactgaat ttatgatgat atggtttcaa agattgacca ccatttttgg
     1381 agaaatgttt gatagtgttt tatcatccga atttgtatct aaaatcaaag atcctgtctc
     1441 taatcagatg ttttctgtag actggctaaa tgaagatgct aagaaatctt tttatgaagg
     1501 gaccattaaa gacgaagaga aatatattaa aatgagtaag aaaatatata ttactgaatt
     1561 tgatgatttt aatgattatc ctttttaagt gaaaatttag aatgaatata aaaaacatta
     1621 caatctaatt tttttaaaaa aatgttatat cggaagtaat ctcttgattt ctatactata
     1681 agataagaaa accactattt aacaaggtta ttcgaacctt attttaagtt acttattatt
     1741 taatgtatcg aaaaaggtga aataggtttg ttataataag aattaagtaa gacatgaatt
     1801 gtctaagcaa atgtagctta tccatttcat taaaatacat atggtgaaaa aacaagttca
     1861 aatagtaact aattttttaa aaaactgatt tataaaaaat acaacacgaa tgaggaaaat
     1921 aaccaatgac agaacaaatc gaaaaaaatt caaaaagctt atatcaggca ctgtggaatt
     1981 ccgcagatat cctgcgttca aaaatggacg ctaatgaata caagagctat ttactaggct
     2041 tggtctttta taaatactta tccgacaaca tgctgcggta tgtctccgtt ctattggagg
     2101 aagaaacgga cgacctaaat aaagcacaag acctttacgt tgaagcattt aaagatgcag
     2161 atataaagga tgatctcttg tatgaactaa aagatgagtt ttcatatacc atcgcacccg
     2221 cattgacctt taccgcgcaa gtggcagcca ttcatgatgg atcttttcaa ttagaagacc
     2281 tggtacaagg tttccgtgac attgaacaat ccagcgaaaa ctttgagaac ttatttgaag
     2341 acattgatct gtattctaaa aaactggggt caaccccaca aaaacaaaat aaaaccatcg
     2401 cagatgttat gaaagaatta gaagggttaa acatggcggg gcatgctggc gatgtcttag
     2461 gcgatgccta tgagtactta attggccaat tcgcatctga atccggtaaa aaagccggtg
     2521 aattctatac cccacaaccg gtcgcaaaat tgatgacaca aattgtttta caaggaaaag
     2581 aagataaaaa aggcttttct gtttacgacg cgaccatggg ttcgggctcg ttacttttga
     2641 acgctaaaaa atattcccat caaccgggaa ccatttctta tttcggacaa gagttgaata
     2701 cgtctaccta taatttggcg cgtatgaaca tgattttgca tggggttccc attgccaacc
     2761 aacacttgca taatgcggat accttggatc aagactggcc aacagaagag ccaacgaact
     2821 tcgatggggt gctcatgaac ccaccgtatt ctgcaaaatg gtcggctgat aaaggcttct
     2881 tagatgaccc acgcttctct gcctatggcg tattagcccc taaatcaaaa gcggactttg
     2941 ctttcttatt acatggctac tatcatttga aagatacggg cgtcatggcc attgttttgc
     3001 cacatggggt gctctttaga gggggcgcag aagggaagat acgcaaagcc ttgttagaaa
     3061 atggtgccat tgacaccgtc attggcttac ctgctaacat cttctttaat acgtccattc
     3121 caacgaccgt aattatcttg aagaaagacc gtgcgaaaaa agatgtgctc tttattgatg
     3181 cgtcacaaga ttttgaaaaa ataaaaacgc aaaatacgct acgagattac catattgacg
     3241 ctattttaga ggcctataaa acccgtacaa ttaaagacaa gtatgcttat gtcgcagaat
     3301 ttgatgaaat tgtggagaat gattacaact taaacattcc ccgttacgtg gatacctttg
     3361 aagaagaa
//
LOCUS       DACBZN010000182         3311 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.186, whole genome shotgun sequence.
ACCESSION   DACBZN010000182 DACBZN010000000
VERSION     DACBZN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.186"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            43..657
                     /locus_tag="IVD64_002836"
     CDS             43..657
                     /locus_tag="IVD64_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6150037.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(822..1826)
                     /locus_tag="IVD64_002837"
     CDS             complement(822..1826)
                     /locus_tag="IVD64_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150038.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(2285..2542)
                     /locus_tag="IVD64_002838"
     CDS             complement(2285..2542)
                     /locus_tag="IVD64_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6150039.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2535..2804)
                     /locus_tag="IVD64_002839"
     CDS             complement(2535..2804)
                     /locus_tag="IVD64_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6150040.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(2935..3114)
                     /locus_tag="IVD64_002840"
     CDS             complement(2935..3114)
                     /locus_tag="IVD64_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150041.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT         1091 a          591 c          448 g         1181 t
ORIGIN      
        1 ttagggtgta ttttaaatag ttgcagaaag taggtaaaag ttttgagagt tgcatacata
       61 cgtgtctcat cgatcgatca aaatgaacaa cgccagattg aagaaatgaa aaaattcggt
      121 gcagagagaa ttttcatcga gaaacaatct ggcgcaacga ttactcatcg acctgttttc
      181 caagaagcat tagattacgt ccgagatcaa gatattttta ttgtggaagc tattgatcga
      241 cttggaagaa attatgacga gatcatcgct tcagtcaatt acttgaagaa gaaaaatgtg
      301 aagctgatca ttactagttt accgatcatg gctgaagcaa ttggcaatcc tctcctggat
      361 aaattcatta aggatctgat catccaaatt ttggccatga tcgctgaaca agaacggact
      421 gaatcgaaac gtcgccaggc acaaggaatt aagatcgcaa aagcaaacgg cgtctacaaa
      481 gggcggccca aattatacag tgcagacgca aaagatcccc aacgtcgcct tgtttacaaa
      541 agcatcgttg aagatcttaa aaatggtgtt gcgattgcta agattgctaa agactacaat
      601 gttacgagac aaaccgtgta tagaataaaa aaagacagca tggtcaatga caaatagttg
      661 atcatgctgc ttttattatc ttctcatctt taatttccta catataatta ctatctataa
      721 aattaagtct tactcagaac agataaagca taaaattcca acaaaccatt ttttacttaa
      781 ctaaataaag agtctgttaa tataatcttc acttatttac gttaatcccc tactgtattt
      841 gttggttcct tatctatatt ttctacaaaa tccttattat cttgtgattt agcttcgtat
      901 aattctttgt atgtcgacac attggttata ctaattccta tagctagttc ttgagactct
      961 acttttctct ctagctcctt tgacaaagtg atttgagatt tatttggttc cgttggagtc
     1021 aattctatag attcttcata aaaatctcta tacataaagc tatcattaat tttataaaaa
     1081 aaattaaatg tttttagcac tgccatataa cattcaaaac attcttctat agttggttta
     1141 cttccttgag gagaatgaac caatttatta cgtttttcta tgaaattttt aatttccttt
     1201 cttatttctt tcggaaactc aatattaaag taccctttaa acatgctccc taataatttg
     1261 tctacagggg gagattgtaa attctcaaat attagagtta aatcaggttt ctcttgttga
     1321 taaaattcct ttacccctat ttcacaggct gtaattatcg aaataaacct atctctagta
     1381 tttgatatat ttttacttct tttaaggagt ataagagaaa aggggatact ttcgtgtcca
     1441 tgcatgtaat taactaattg ttgagcttca gaagttatat ctctaattat atagttaacg
     1501 gaattaggaa gtgtctttaa ctctaatgaa atttctatcc aattactatc ttctaatctg
     1561 tatatgctcg aagtataatt tgattcgtca gcaagcatcg ctacatccca aacactgatc
     1621 ataaaatctt ttaaacgttc aatcatttca gcaatattgc taaaatcagc taatttttct
     1681 ttagaataaa aaaaaacatt tatatcagaa tcaataaatt ctattttttt tattactata
     1741 tcgtcatcat tatcttgacc atttatgttt attgttccct gagtggggag tacttctttc
     1801 aaattcaagt taaatttata ttccattact tctcctccaa tatataaaaa ttacttaact
     1861 gtatccaaat ttaatatgta aataaatcat taatttttag ctaaattcta tgagaagttt
     1921 atttttgttg tttttgctaa ttaactaaaa ctagtacaaa aaatttaaaa tagattatta
     1981 aacctattgc ttattctatt cagtcatata tataagcgtc atcctttagt aactaacatc
     2041 ccacactaca atagtattat aacatatcta ttcaacttca aattgttgaa atttaagcca
     2101 tttatattta cttttcctca actttagaaa tgattttact aaccttatat ccgtaatacg
     2161 cgtaacaaac acattcatta ataacaatca acactaagct atattagctt tttaaatgac
     2221 tagggtaaat aaaataagga tcaaaaactt ttaagtttct gaccctttcc ttacttccga
     2281 ttggttaata gtgatctttt gcagaataaa taaatatcgt ttcattttca actctatata
     2341 tcagtctatg ttcatctgta attcttctgg accattttcc agataaatca tgctttaatg
     2401 gctcaggttt tcctaatcca gcaaaggggg aacgatcgat atcttttatt aacttgttaa
     2461 tcttttttat attgcttttg tttccttgct catgccaata aagataatca tcccaagcat
     2521 catcagacca agccttaatc atcagattca acctcgatta agtcatgtgt tttaaatgca
     2581 cctttggaga attgttcatc tcctcgacga attttttcca tgacgtaatt attagaaagt
     2641 gttctcaacg tttcttgcat agaatcataa tctctttttg ataatacaac aactgtatct
     2701 tctacatctt tacttgttac aataagtgtt tcagcatcct cattaacttg tttcatataa
     2761 ctacgtaaat tttggcggaa atttgaataa gctactgctt ccattccttt caccccttat
     2821 ttatttctat aaaacaattg tacattatat tgtacaatta agcaaatgat taatattcta
     2881 acttcttata attaaaagtg ctatcacttt tagtttttag gataaaaagt gacgtcactt
     2941 tttgagtgct ggtacctcaa atcctgcttt ttcttgggca atcaataaat ctaacgcaat
     3001 acacataatc ctggattcac ttttgccgta cttctgcttt agttgctgca attctttttg
     3061 tctcttttca tccaacgtaa cgggtaaccg tttatttcct attgcaattg gcattagtac
     3121 tctctccttt catcgaaata accctatgaa aaatatggct gcaatcatct tgaatagtag
     3181 actcacaagg gctaatacaa agcgccaaaa gatcaaacaa aataccagta ttgccaaaaa
     3241 tactacgaag tttaaatgca cagctgcagc atggcgcata agtatttcgc caacaaatag
     3301 gactaacaat a
//
LOCUS       DACBZN010000183         3272 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.187, whole genome shotgun sequence.
ACCESSION   DACBZN010000183 DACBZN010000000
VERSION     DACBZN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.187"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            2..586
                     /locus_tag="IVD64_002841"
     CDS             2..586
                     /locus_tag="IVD64_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6150042.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(663..1838)
                     /locus_tag="IVD64_002842"
     CDS             complement(663..1838)
                     /locus_tag="IVD64_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6150043.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1920..2300
                     /locus_tag="IVD64_002843"
     CDS             1920..2300
                     /locus_tag="IVD64_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP6150044.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2374..2736
                     /locus_tag="IVD64_002844"
     CDS             2374..2736
                     /locus_tag="IVD64_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150045.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1110 a          535 c          620 g         1007 t
ORIGIN      
        1 tatggcattc ccacaactag atttaaaaag tgaaaaagga ccaaaagctg ttatcaaaac
       61 aaaccgtggt gacattactg ttcaattatt ccctgaacta gcaccgaaga cggttcaaaa
      121 tttcattgaa ctttccaaaa aaggttatta tgatggcgtg atttttcacc gcgttattcc
      181 tgatttcatg atccaaggcg gcgatccaac tggaacagga atgggtggcg aaagtattta
      241 tggcgaaagt tttgaagatg aattcagtcg tgaactgttc aaccttcgcg gagctttatc
      301 tatggcaaat tcaggtccaa acacaaacgg aagccaattc tttattgtaa ataacacaaa
      361 tgtgccagct aatatgcttg gacaactaga aggtgctgga ttcccatcag aaatcattga
      421 agcgtataaa ggtggcggaa ctccttggct agatttccga catacagtct ttggacatgt
      481 actagaaggg atggacacag tagatgaaat cgcaaatgta caacgtggtc cacaagaccg
      541 acctgtacat gatgtagtga ttgaaacaat tgaaatttca gaatagtttg gtatggtacg
      601 gagtaattat gaaaaaggac gaaacctagt aaggtctcgt ccttttattt tgaataaatg
      661 atttaatcga ttgtctctaa agcatacttg attcgattaa gtccttccag taaagtttct
      721 tctggacaag caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa cgtgatgcca
      781 ggatttaata cgacttttcc tttccgaaca agagtgttct ccaattcttg gtcgtctgaa
      841 atataggcgg taaaatctaa ccacattaag taagttcctt cagggttcat tattctcact
      901 ttcggaagat actgttcaaa aaactgatta actgtttgaa catttttttc tagataagga
      961 atcagttggc ttaaccattc tccacccgtc tcataagccg cttgggttcc aatcagtcca
     1021 aaagtgttga tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc agctttcatc
     1081 tctggatttt tgataaatac cattgaattt ttgattgcag ctagattgaa agtcttggtt
     1141 gcggaagtaa aagcaactag tagatcatct aaattaggat caatcgttag catggaagta
     1201 aatgtatgat cgaataaagt taaatcttga tggatctcgt cactaaacag aatcacttga
     1261 tgttttaaac acaattcgct taactgtttc aattcttctt tgctccaaac acgccctcca
     1321 ggattgtgag gattgcataa aatcattgct tttacattgt tttcttcgat ttttttctcc
     1381 atgtcagaca agtccatgat gaaatgatta tttttttcta gcaaagagct tcgaacgatg
     1441 tgacgttgat ttgtttctat aatagaggaa aatggaggat aaactgggtc atgaatcaaa
     1501 atagaatcgc ctggttttgt aaaagtttgt actgctgcag ctaaactggt taacacacca
     1561 cttgtaaaaa cgatattttc tttcgtcaac tccacagcat ggtgttggcg ttcccaatga
     1621 atgatagcct gatatagttt atcagacaga gtggcataac caaagattcc atgtttgatg
     1681 tattcactaa acgctttagc taccccaccg ggagacaaga agtccatatc tgctacccac
     1741 aaaggaagta agtcatccat ttgatatgtc tcagcaattg cgtcccattt tacactttct
     1801 gtatcttttc ttgaaatctg tttatcaaat tcgatcatct taagacctcc agtcattttc
     1861 gtcgcttctt tttttgagct atttacagta taatgataaa tgcgaagaga gggaagtaga
     1921 tgacttataa aattggacaa ataattgaag gaaaagtgac agggatacag ccttatggcg
     1981 cattcgtttc tcttgataac gagacgcaag gacttattca tgtttcagaa gtacaatccg
     2041 gttatacgaa aaatatccat tcattgttaa aagtcggaca gccagtaaca gttcagatca
     2101 tcgacattga tgaatattcc aaaaaaatca gtttatcttt aagaacgtta gaaaaaacaa
     2161 atccagctgt tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt aagatcggat
     2221 ttaccactat tgcggagcag ttacctattt ggatagaaga agcattatct gaactagaac
     2281 ggaaagaaaa taagaaataa aaagtgtatt ttttctgaaa atgtgctaga atttttatgt
     2341 aagccataga acaagttgat cgaggtggaa attttggcaa gcaaaagagt tgctggtaca
     2401 attatgttgc atttggagga tggtactaaa aaatttttgg tccattcaat tgatgacaag
     2461 ttggaatttg cgttagcgga actttctgaa ggaaagactg gtttagcgaa tatacttaat
     2521 ttcttaaaag aaatagttca tatcgatgtt agcaagatta gtttgatgga attgacaaat
     2581 acacatatta aagaggaaaa tgttccgttg tttgtctttg aaactgaaca aaagaatcaa
     2641 agagaagaat taggcgaagg atatatttgg gaagaaccag gtcaaatgcg ctctgttctt
     2701 ggaacttacg aagtagaggg agtgcctttc ttctaaaata cagattaagc gaacaaagaa
     2761 gtttgaattt ttttcaaact tcttttttta ttgtttggtc caaaaaatac aatgaaatgt
     2821 tctgctaaag atagtttttt agtgcatttt attttcaaaa tatgttgaaa acggttgtat
     2881 aacgaataat aataattgat cgaaaaatca tttcacaaag taatgcttaa tttatagttg
     2941 accaaggaag aaatacatga taaatttaac agcgttgatt ctgaagtgga tgttatatca
     3001 catttctaaa aaatttaaaa aatagttatt gacatgttac taacgacatg ataagataac
     3061 tgagtcgtta aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa cttcaaaaaa
     3121 gttcttgaca aataacaaac gataagttat gataaatgag ttgctgaaag atttcgaaaa
     3181 acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg cttgtctctc tgatatgata
     3241 taaaagttgc tacggcagcc aaaaacgaat cg
//
LOCUS       DACBZN010000184         3268 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.188, whole genome shotgun sequence.
ACCESSION   DACBZN010000184 DACBZN010000000
VERSION     DACBZN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.188"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            204..1226
                     /locus_tag="IVD64_002845"
     CDS             204..1226
                     /locus_tag="IVD64_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6150046.1"
                     /translation="MIAELKPKYERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1237..>3268
                     /gene="topB"
                     /locus_tag="IVD64_002846"
     CDS             1237..>3268
                     /gene="topB"
                     /locus_tag="IVD64_002846"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6150047.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQ"
BASE COUNT         1188 a          600 c          712 g          768 t
ORIGIN      
        1 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
       61 gagtggaaaa tcttcatttt gaaacagaaa agtacaaaga tcaccccgtt gattatcaac
      121 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga
      181 ttgattatgc agaaaaagaa gggttgattg ccgaactaaa gccaaaatat gaacggcaaa
      241 atttcttagt tcgagacgat cgtttagatc atgcggtcgc ctttttatgg aaagatccac
      301 agacaaaaga gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg
      361 gtgagagagg cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc
      421 taaagatcgg tgaccctaag cagttaaaat tttttgagag tagtattgat ttacttagct
      481 atgctgcact gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa
      541 aacaccatgt gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag
      601 cctttcctca atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg
      661 aaaaagaaca attaatgggc gcagtcgatc catttacgaa tcagaaagta cgttgtgaac
      721 gaggaatcgc taatgattgg caagtgccaa aagaatacaa aatcatttat gaagaagtcg
      781 caaaagaaga aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc
      841 tgcaattaac gaaccaactg gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc
      901 tttctgttaa cgattcaata gaagccatta atttgaaaga tatttgtaga gaagtcgcaa
      961 aagaattaaa agtttgtgaa cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag
     1021 aaaaagggga tattaatgcg cagattcttt tctcttataa agcagaacaa tactacaaag
     1081 gctataaaaa tcatgaacat gaatttatac ctgaagtaaa aaaggactgg aacgatcaat
     1141 tgaagcatga gatccagcaa caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac
     1201 aaggcagaca acaagaaagg gagtgaaatc gtcatgatga aaaccgttat tttagcagaa
     1261 aagccgtcac aagcgaaagc ttatgccgat agcttctcta aagccactcg aaaagacggg
     1321 tattttgaaa tccaagatcg attgtttcct ggtgaaactg tgatcacgta tggttttggt
     1381 catttagtcg aattagattc gcccgatatg tacgatgaaa attggaaaca atggtcatta
     1441 gaacatttgc ctattttccc tacacattat cactatcatg ttcctaaaga taagaaaaag
     1501 caattcaagg tggtgaaaca gcaacttcaa tcggccgaca ccattattat tgcgacagat
     1561 agcgatcgtg aaggggaact gatcgcttgg acgatcattc aacaagtagg cgccgatcaa
     1621 ggaaaaacct ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga
     1681 ttccagcagt tacgagacgc aaaagagacc tatccaaaat ttgaagaagc acaagcacgt
     1741 cagatcgccg attggttaat tgggatgaac ggtagcccac tgtatagttt actcttgcag
     1801 caaaaaggca taccaggaag cttttcctta ggcagagtcc aaaccccaac gctttacatg
     1861 atttatcaat tgcaggaaaa gattagaaat ttcaagaaag aaccttattt tgaaggaaaa
     1921 tccgaagtaa tcgctcaaaa tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa
     1981 gcgacgcaag aagcatttga agattattta aaagaaaagg gggttcaatt gggaaaacag
     2041 ccaggcatga ttcgccaagt tgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt
     2101 tctctatcca gccttcagtc taaaatgaat cagctcatga aagctagtgc caaagacact
     2161 ttggaagcca tgcaaggctt gtatgaaggg aaatatctaa gctatccacg aacagacacc
     2221 ccttatatta cggaagggga gtatgcgtat ttgttggatc atttagatga atacaagcat
     2281 tttttaaagg ccgaatcaat cccaacccct attcatacgc cgaatagtcg gtatgtgaat
     2341 aacaagaaag tccaggaaca ttacgcgatc attcctacaa aaacggttat gacagcagcg
     2401 gcttttgaac aactaagtcc actgcagcag gccatttatg aacaagtgtt aaaaacaacg
     2461 gtggccatgt ttgcggaaaa atatacctat gaagaaacaa cgatcttgac gcaagtccag
     2521 cagcttcagt taaaggcaat cggaaaagta ccgatagatc taggttggaa gaagttattt
     2581 ggcaaagaga acgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa
     2641 ggagaaatgg tgacggtaga tctacaagtc cttgaaaaag aaaccaagcc accacagcct
     2701 tataccgaag gtacgttgat cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa
     2761 gaagcgcaat ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat
     2821 attattgaaa ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg
     2881 acgaataaag ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagtgcc
     2941 gaaatgaccg ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa
     3001 accacttttc tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg
     3061 caaatccaat ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag
     3121 caagaagccg cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc
     3181 tatcaaaagg tggcagcatg tacgtctgaa gcctgtgatt tcaaactatg gacaacgatt
     3241 gccaaaaaga aacttacagc gacacagc
//
LOCUS       DACBZN010000185         3267 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.189, whole genome shotgun sequence.
ACCESSION   DACBZN010000185 DACBZN010000000
VERSION     DACBZN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.189"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            7..720
                     /locus_tag="IVD64_002847"
     CDS             7..720
                     /locus_tag="IVD64_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6150048.1"
                     /translation="MGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAVEIFNFLSGYF
                     GGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIFGGIFDGIVAV
                     GKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGGHLINGQAIVG
                     EAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDANNPSELDRMN
                     RKFAKANKQAIRDLGGVPI"
     gene            732..1436
                     /locus_tag="IVD64_002848"
     CDS             732..1436
                     /locus_tag="IVD64_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6150049.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            1433..>3267
                     /locus_tag="IVD64_002849"
     CDS             1433..>3267
                     /locus_tag="IVD64_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150050.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT         1096 a          493 c          736 g          942 t
ORIGIN      
        1 atagctattg gcgcattagt tactggacta atttgggcat ataataacgt taaatggttt
       61 catgatggag tgaattcttt cttcaaaggt gtttcagatg tagctgttga aatatttaat
      121 tttttaagtg gttattttgg tggatttttc gatggtgtta tagcaaattt caataatttt
      181 tataatgctg gaaaacgaat tttcagtggt tttctagatt ttatcactgg agtatttaca
      241 gggaattggt caaaagcatg gcaaggtcta gttgatattt ttggaggtat tttcgatggt
      301 attgttgctg tagggaaagc tcctattaat gccatgattg gattgatcaa tggatttatt
      361 ggtggattga ataatataaa gataccaaaa tgggttcctg gtgtaggtgg aaaatcattt
      421 tctatttcaa aactgcctta tttagcacaa ggcggtcatc taatcaatgg acaagcgatt
      481 gttggtgaag ctggacctga gttactgact gccaagaatg gtaaaaccac agttactcca
      541 ttatcagacg aagaaaaacg caaagggatc agtggaagag tttctggagg tagcattgaa
      601 cagcatatac acattggcaa agtcgatgca aacaatccat ctgagttaga tcgtatgaat
      661 cgtaaatttg ctaaagcaaa caaacaggct attcgtgatt tgggaggtgt tcctatttga
      721 gtcgacaatt tatgaatcca gatgaaccaa attttatttg gaaaaatcgg aatgcagtaa
      781 ttgatatgaa ttgcatcatt gaggcagagc ttccagatat tatgccgaac aagcgatacg
      841 aaacttacac gattcagggc cgaagcggag aattgacgga gacgtttgat gactatgaac
      901 cttttgattt ggaaatagaa gggatcacta ttccacattc gaaactgaga gaagtcaaac
      961 gatggctcac tggtaaaagc caattaatca cacataatga tccagacaag tatctagagg
     1021 ctatctgcag catggataaa gaagtccctt tcgaaaacga gtggggcttc ttttatacat
     1081 tcgatgttac ttttcgttgt cagccactca aaagaaaaat cggtgaagca cctaaaattt
     1141 ttcattctag tgttatggat atttttgatc atggtgacga aattgcacat ccctatctgg
     1201 aaatagaatc gaacggagga gatataacga tcgctgtcgg tgataaaagc ctaacaatac
     1261 ttaatacgtt agctgcaaca atcgtggttg atactcagct tggaaaagca atacaagaag
     1321 gtttgaattt atttacgcgt ggtgattggc cagtattgca gcctgaatgg aatcaggtaa
     1381 aaatatccgg aaaatttaaa gaagtgcggt tttggaatag gagtgtgtat ctgtgacgca
     1441 agaatttatt tatgcttata aaaaaatgcc agatgatttg agcgtgaacg gagcgtcgtt
     1501 agttgattgg gaagatttgc cagaaatcaa tcgtgtgctg aatggccaat atcgttttta
     1561 tggtaactat tcaagaagcg gacagtatcg ctcttattta aaaaaaggaa attttataaa
     1621 agcgaaggta ccagatggat catggcaata ttttgaaatt tataatgtca aaaagaattt
     1681 gacctccgtt tctgtaacag ctcggcatat tggttttatg gccaataaga actttattgt
     1741 caagtcgttt acggataacg gcaatgggtc tcagatcatg accaatctta aaaacagttt
     1801 ggcatttgat caaaaattta actatctttc gaatgtaggg acaacgcacc aatttacagc
     1861 aagacaagtc gcgccagttg aagcaatcat tggttcaaat aacggtaatc aaaatttgac
     1921 tggcgtgact agcgcggaac ttgatatgga taactatgat ttgaaactgg taaaacagat
     1981 tggatcagat aatggttttc ggattgattt tggcatcaat ttagaagcaa tcgaagaaga
     2041 aattgatgaa gaatcaattg tcaatagttt gtatttggtt ggcggtgttc ctgataacga
     2101 ttatgatgaa gacaaagaac ctatcgaata tggttattta gaaattgatg gagtaaccaa
     2161 cgagaatcga cgtattgcca aacgtgaaaa cggagattgt aaaacagttg atgaattgat
     2221 caaatggggc aaaaccttat ttgacaatga tcgtatccat gagccaaaag ctactcatac
     2281 agtcagtatg gtggcgttgg aacacacact cgaatacggt gacatgtatc aagagctagc
     2341 gtctttacac tttggtgatg tcgctcatgt tagggcgaaa gagctagata tcgagataaa
     2401 agagcgtatg gtcgaataca cttatttccc aacgcttggg aaatataagg atattgtttt
     2461 aggtaatgat ttatcactgt acacttcggc agtaaatact caagcacaag aattgaaaaa
     2521 gaaaatcgac aatcgaacag aaacgttagt tcaaaatgtg ttgaatgcga cggcatggat
     2581 cactggtaat tctggtgggc atgtcgtttt tcgtccagaa aaagctccat cagagattct
     2641 tatcatggat actccaaatg tggcgaatgc aaagcgagtg tggcgttgga atttaaatgg
     2701 cttaggttat tcagacaacg gagtaaatgg cccgttcggt attgctatca catcaaaagg
     2761 agaaattgtt gctgacttca tcaaagtagg aacaataaat gcagaagtat tcgaaacttc
     2821 ctttaatgct tatggtgatg tgttaaaact tgtaaaaggc acgcttcaaa tttggaatga
     2881 aaacaaaaaa atcatggagc taaccaaaaa aggtatggaa ttctggaata gaaaagaatc
     2941 cattggaacg atcggaacaa cagattcagc aggaaatcca tttccaaatg ctgtaacacc
     3001 tacacctctt gaggaaaatt ctttggtaat acgtacaaat ggcgatggaa aatacatttt
     3061 aatttctcca actgcagaaa aaggatttgt gttattagga aacggaaaag catattattt
     3121 cggtgattta gatattcagg gaaaacttac tgttagaggt aaagaagtga taccaggaca
     3181 aaatggcggt ccatctggcg gaggagaaac acctggtggg tatcctgatg aattaaaaac
     3241 agatgcagaa aagagagctt ggagaat
//
LOCUS       DACBZN010000186         3178 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.190, whole genome shotgun sequence.
ACCESSION   DACBZN010000186 DACBZN010000000
VERSION     DACBZN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.190"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(6..977)
                     /locus_tag="IVD64_002850"
     CDS             complement(6..977)
                     /locus_tag="IVD64_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP6150051.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1683..3062
                     /gene="brnQ"
                     /locus_tag="IVD64_002851"
     CDS             1683..3062
                     /gene="brnQ"
                     /locus_tag="IVD64_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6150052.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT          894 a          633 c          593 g         1058 t
ORIGIN      
        1 aaagcttatt cttgatggag atctgccttt tcataagctt tgatgatttc ggcaactaca
       61 gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat cgattttctt gatttgcttt
      121 aatgttcgtt ctgcatgaac aagaccactt gttgtccctc gaggcagatc aatctgactc
      181 gtatctccat tgacaatcat ttttgattga taaccaagac gtgtcaggaa cattttcatc
      241 tgagcaactg tcgtgttctg tgcttcatcc aaaataacga atgcatcatc caatgtccgc
      301 ccccgcatgt aagctaaagg agcaatttcg atcacgcctc gttccatcaa tcggttcgta
      361 tgatctaatc caaaaatttg gtataaagca tcataaacag gacgcagata aggatctact
      421 ttttctttta gatccccggg aagaaagcct agattttccc ctgcttccac agcaggccgc
      481 gtcaaaatga ttttttgcac ttcacctttt ttcaaggcag cgattgctaa tactactgca
      541 agaaaagttt ttcctgtccc ggctggtcct acgccaaata cgatatcatg tttggctacc
      601 gattcgacat atttcttttg gccgctgttt tttacccgta ttggttttcc attacggtct
      661 ttgacgatct cttcctcata catttcaaca aaataatcta atgtgccttt ttgccccatt
      721 ttcagtgccg tcacgacatc tggtgtactt acatggattc ctcgtttgat caattcctgc
      781 aatgtccgaa tgactgaggc agccaaggaa atctgggatt gttcgccaat gatctgaatg
      841 atttctccac gtgtatggat ggttgtttct gttgtttctt caatcagttt tatatgcttg
      901 tcgtgtgcac ctaaaagcat gctgacatca tcagcagccg tcaatctaat ctctaaagaa
      961 ctagactctt ccgtcaaaaa tagacatccc ctttttttcg attcattctt tcctatttta
     1021 ccatactcag aagaaactat atagcaaata agaaaaggtt ctaaaagagt tgcttaacca
     1081 taagtaccaa gaaacgttaa aaaatagctg cttccattct ctaaaggtct cgtttgaata
     1141 aaaaaagctg atcatgtaag ctatgcataa gatagaggac aagtattccg tctaacttag
     1201 caagtattct ctgggtattt ttcttgtttc aaaaaaagaa aacaaaatat ttgaaatatt
     1261 cttgcatttc aaaaaaaaca ggtgtattat aacgtcaaca aataaatata cagcaaagcg
     1321 tcgaggaaaa cagtaggcta ttcagcgatt tattcccagt gagtcatgta agtgagaaat
     1381 gacaataaat cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg tagatcattg
     1441 tggctgcctc cattatcagg ctagcggata actttatcga aaagcgtccg tgaatgaaga
     1501 agctttttta agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt ttcccaagtg
     1561 gaaaagggct tttttttata ttttaaggtg gtggttttca tggaatctga tattagtggc
     1621 atttctagtt tctgcaacgt ttattttgtt actcaaaatg ataattattg gaggaaaaat
     1681 ttatggaaga aacaaaaact tattcatgga agcaattatt gatggtcagt tcacttatct
     1741 tcgggatgtt cttcgggtca ggtaatctga tctttcctgt ccacttaggt caaatggctg
     1801 gatcaaactg gatcagcgct ggtgtaggat ttgccatctc aggcgctctt ttcccattat
     1861 tagctatctt agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg
     1921 tcggaaaatg gtatgctgcc ttgttcttga tccttgttca tttaacgatc ggaccattct
     1981 ttggtacacc gcggacagca gcaactgctt ttgaaatggc tgctaaaccc tttttgcctg
     2041 aaaaatatga ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt
     2101 tccttactgt acatccaaca cgtttggtca aatatgtcgg aaagtattta aactccttgt
     2161 ttcttgcatt attagctatc atttttgtcg tttctttatt ccaacccatg gggaatctaa
     2221 accaatctgc tgcagattct tatcagacga acgctttgac aaatggcgtt ttagaaggat
     2281 acaacacggt tgatgcagta gctttacttg ctttaagtgt cacttttgtc catgcagttc
     2341 gtgatcttgg ttttaaagat aaaaaagtca cagaattaac agcaaaagct ggtagcctga
     2401 gcgttgcgtt agaagtcatg atttattttg gtctggtcct tctcggtgcg atgagtttga
     2461 ataagatgcc tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt
     2521 taggtaactt cggtgctagc ttcttagggg taatggtcac attaggtgtc ttcactaccg
     2581 ctatgggact cgttgtttct tttgcacaag atttccataa actgttccct aaagtcagtt
     2641 atatgacatg gctgcggtta acgacttttg tctcttttgt cgttgcgaat gccggattgg
     2701 ataatatcat ccaatggtct ttaccagtat tgatgctttt gtatccattg tcacttgcgt
     2761 taatcttgct ttccttaaca gcaaaattct tccaaaagag tccatttgtc tatcaagtaa
     2821 cgatgttctt ttctgccgtt ccagccgtat tggatatgtt ggcaaattcg cctgcattag
     2881 tgagtcagca aactgtcgta gcaagcttac taggatttta tcatcatcac gttccttttg
     2941 ctgcattagg acttggctgg atggtcccaa cgttacttgg ttacgctggc agcttactct
     3001 tttattacgc ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat
     3061 aaccaaatca aacacaattt aacataagag atattaaaca ggaaaaaaac agctagaaga
     3121 aagaataaga ttctttcttc tagctgtttt ttagttcttt ttttacgaac acaaaaaa
//
LOCUS       DACBZN010000187         3111 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.191, whole genome shotgun sequence.
ACCESSION   DACBZN010000187 DACBZN010000000
VERSION     DACBZN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.191"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..1350
                     /locus_tag="IVD64_002852"
     CDS             <1..1350
                     /locus_tag="IVD64_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6150053.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="IVD64_002853"
     CDS             1484..2827
                     /locus_tag="IVD64_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6150054.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..>3111
                     /locus_tag="IVD64_002854"
     CDS             2939..>3111
                     /locus_tag="IVD64_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6150055.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1232 a          430 c          589 g          860 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt t
//
LOCUS       DACBZN010000188         3020 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.193, whole genome shotgun sequence.
ACCESSION   DACBZN010000188 DACBZN010000000
VERSION     DACBZN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.193"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            341..439
                     /locus_tag="IVD64_002855"
     CDS             341..439
                     /locus_tag="IVD64_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6150056.1"
                     /translation="MLEKLITLVIAPLFVGLVIELISHWLDEKDDN"
     gene            1163..1444
                     /locus_tag="IVD64_002856"
     CDS             1163..1444
                     /locus_tag="IVD64_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150057.1"
                     /translation="MNILIRNIDPKIITQIDKRCQELTNKTGKKWSRNQYLKVLIEND
                     FDRKLMAYRKDQFDLLLEKFIDIQAINTQVLSDYIHTNNQLIKTLIGGI"
     gene            1446..1862
                     /locus_tag="IVD64_002857"
     CDS             1446..1862
                     /locus_tag="IVD64_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150058.1"
                     /translation="MRKTSIQKHITLYPSHVDVLDSIIEKNPDINNYSEAVRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMAEIIAEFADLYLKDTGVLIGTKSEVYQEVKRKI
                     EKQLNPSQRNQPKNHQKETNQTVTSRNFDPTKFLKR"
     gene            complement(1951..2673)
                     /locus_tag="IVD64_002858"
     CDS             complement(1951..2673)
                     /locus_tag="IVD64_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HAP6150059.1"
                     /translation="MNKNITKARHFGFLLYPESIPENWREQLESLGVSMAVSPLHDMD
                     EKTNKKDWTSSDVIRNGKHYVKPHYHVLYIARNPVTIESVRKKIKRKLGNNSVAHLEI
                     IDHIKGSYEYLTHESKDAKEKNKHVYDKKDILHINDFDIDRYVTLDESQKKELRNQLF
                     DLVNHCGIVNMKELAAFLDWRGQEYGITNQRDVVDVIASNSNLFRMMFDANYQNGFRP
                     QYAKRIDSETGEMIEGVNKHVE"
     gene            complement(2754..2903)
                     /locus_tag="IVD64_002859"
     CDS             complement(2754..2903)
                     /locus_tag="IVD64_002859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150060.1"
                     /translation="MAESERLVVILPSEIKRKLEEKAKDKSLSMSTYVKLLVTEDLKK
                     SEKGN"
BASE COUNT          953 a          638 c          446 g          983 t
ORIGIN      
        1 aaaaaaaaag gctctatcca ttggtacagt aaggataaag cactttttgg cacaaaaatt
       61 ggcactcggc acactatggg gggtcgtagt acggaagcaa aattcgcttc ctcgcccccc
      121 agttgtccca aattccaaat ttttttcaaa aattttccag cgcttccgct cggcaaaatc
      181 gcaagcgatt ttaacactgc ctttttaagg gggtgttcaa attttgaaca tttaaccgcc
      241 tgttagaaat acggttaagt ttttcccact cattctcaaa gaaaaaaaga gatatgctat
      301 aatcacacat ggcaacttgc cccgaaaggg ggtgttctct ttgctagaaa aactcatcac
      361 gttagttatc gcaccacttt ttgtagggtt agtcatcgaa ctaatttctc actggttaga
      421 cgagaaagac gataactaaa gcaagttgct ttctaatcca cacgtttgtt tccaccacaa
      481 gcgcaaaaaa atcccaacct acggacatag gttgggattt tgtgttctct tgctcttgca
      541 aaaactcatc acgtttccac actgctatta taacaagcaa taactgaaaa atcaaagaca
      601 caagaccttg gggaatgtca ttccccaaac cccttgagcc tcctcctgcc gaaatatttc
      661 tacgctctcg ctcctcattt cattcagttc tcggctccac aatctaacac gccgtttcgc
      721 taccagtcgt ctttcagact ccattctcgc tcctctcacg tgttttgacg attgcgccga
      781 taactaaacg aaataaacgc taaaacgtct tagaacgcaa aatatgagct tctaagacta
      841 ttccatttca gttacatact ctttataatc gttccatttg atttctcttt cgtgccattc
      901 attatcgctt ttcgcaaaca gaacagcacg aatccatgtc actatttttt tgttttttaa
      961 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     1021 cacgtttctt tgaaaaaaat ttgatatcac ttttttagcg ctatgtctag gctatgtata
     1081 gtttttaggc aaaaagcagc tatacatgta ctatacttgt atgatataca tgaattatac
     1141 atagttgcaa aggagacttt ttatgaacat tttaattcga aatatcgacc caaaaattat
     1201 cacgcaaata gacaaacgct gccaagaact tacgaacaaa actggcaaaa aatggagtcg
     1261 aaatcaatac ttaaaagttc taatcgaaaa tgattttgac cgtaaactta tggcttatag
     1321 aaaagatcaa ttcgatttat tactcgaaaa atttattgat attcaagcaa taaatactca
     1381 agtattgagt gattacattc acacaaataa tcaacttatt aaaacactta tcggaggaat
     1441 ctagcatgag aaaaacttct attcaaaaac acattactct ttatccaagc cacgttgacg
     1501 ttttagattc aatcatcgaa aaaaatccag acatcaacaa ttactcagaa gctgttcgtt
     1561 ttttatgtat gagttatgaa cctgatacca acacgaccac taccagcaat caggcaaaac
     1621 taaatgctat gggaaaagaa atttctatca tggctgaaat catcgcagaa tttgccgatt
     1681 tatatctaaa agatacaggc gttcttatcg gtacaaaatc tgaagtgtat caagaagtga
     1741 aaagaaaaat agaaaaacaa ttgaatccct cacaacgaaa tcaaccaaag aatcatcaaa
     1801 aagaaacaaa ccaaacagtc acttctagaa atttcgatcc aacaaaattt ctaaaacgat
     1861 aaaaaaacag ataggagtta ttcctatctg ttttttattt cccaatattc tattttttca
     1921 ttccaaacag ttaacggaag ttttcgatat ttattcgacg tgtttattca cgccttcaat
     1981 catttctcca gtttcagaat ctatcctttt tgcatattgc ggccgaaatc cgttctggta
     2041 attagcatca aacatcatac ggaaaagatt gctgtttgaa gctatgacat caacaacatc
     2101 tctttgatta gtgattccat actcttgacc tctccagtca agaaatgcag ctaactcttt
     2161 catattgact attccacaat gattaacaag atcaaacaat tggtttctca actctttctt
     2221 ttgactctcg tccaaagtta cataacgatc aatatcaaaa tcattaatat gcaagatatc
     2281 ttttttatcg tatacatgtt tattcttttc ttttgcatct tttgattcat gcgtaagata
     2341 ctcatatgag cctttgatat ggtcaattat ttctaagtga gcaaccgaat tatttcccaa
     2401 ttttctcttg attttctttc ttacgctctc gattgttaca ggattgcgag caatataaag
     2461 gacatgataa tgtggcttta cataatgctt accgttcctg ataacatcac tactagtcca
     2521 atccttttta tttgtttttt cgtccatatc gtgtaacgga ctaaccgcca ttgatactcc
     2581 taagctctca agctgttctc tccaattctc tggaatagat tcgggatata gtaaaaaacc
     2641 aaaatgtcta gctttcgtta tattcttatt catgatatac ttacctcaat tgaacactat
     2701 cagttaagcg gaaacggtgc gccaacacat atgtttctgc tctttttttg cgctcaattt
     2761 cctttctctg attttttcaa gtcttctgtt actagcaatt taacatatgt cgacatactc
     2821 aaacttttgt cttttgcttt ttcctcaagt tttcgcttaa tttcactcgg taaaattact
     2881 accagtcttt ctgattccgc catgcctttc agccccttta gttaattgtt aatatcatgg
     2941 taaacttttg acaactgtat tgtcaatatt tttttctatt tttcaaaacg gcggtcaaaa
     3001 cggcggtcaa atggcggtcg
//
LOCUS       DACBZN010000189         3010 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.194, whole genome shotgun sequence.
ACCESSION   DACBZN010000189 DACBZN010000000
VERSION     DACBZN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.194"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(106..1053)
                     /locus_tag="IVD64_002860"
     CDS             complement(106..1053)
                     /locus_tag="IVD64_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6150061.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IVD64_002861"
     CDS             1299..1763
                     /locus_tag="IVD64_002861"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP6150062.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IVD64_002862"
     CDS             complement(1825..2868)
                     /locus_tag="IVD64_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP6150063.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          996 a          596 c          576 g          842 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta
//
LOCUS       DACBZN010000190         2987 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.195, whole genome shotgun sequence.
ACCESSION   DACBZN010000190 DACBZN010000000
VERSION     DACBZN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.195"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            287..694
                     /locus_tag="IVD64_002863"
     CDS             287..694
                     /locus_tag="IVD64_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150064.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVD64_002864"
     CDS             708..1109
                     /locus_tag="IVD64_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150065.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVD64_002865"
     CDS             1111..1482
                     /locus_tag="IVD64_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150066.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IVD64_002866"
     CDS             complement(1518..1820)
                     /locus_tag="IVD64_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150067.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1916..2710)
                     /locus_tag="IVD64_002867"
     CDS             complement(1916..2710)
                     /locus_tag="IVD64_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP6150068.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT         1053 a          424 c          458 g         1052 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat cgaatttaaa ccataattgt
     1801 gtttttcctt ccattaacat ataaagttca cctcaaaaag agtatacgaa caaatgtttg
     1861 tattgtaaat agaaaaagat gagctaaatt aaatgcccat cttctttgat agatattata
     1921 aatctaataa ttcttgtttc tttttattaa attcttcttc tgtaatgatt ccatcatcca
     1981 atagttcttt atattttctt atttctgaag ctgaggaaat tgttgatgta ttggaatttg
     2041 tagcagaaat atttcttctt tttaaattat aaataatttc gtttaaagaa tcacttatcc
     2101 tatcaacagt atctttatca actgcaacat tcaatacttt accaagagta tcaatagtta
     2161 atgtacccaa cagcactcct ctttttttac taatattatt taaattgtct aatataatac
     2221 tttgtacatt attgcctatc attttttgtt gagctacaat taaacgttta ttagttaatg
     2281 cgtatgcata attattgtca tgcttagttg atgagatata attatgcaat cctacaaagc
     2341 agaagattac gtattcgtca ctgttaagtt gatctgatat taagttaaaa tgatgagttg
     2401 tccatttttt agtcattcct ttaccgtagc cgtatttaag acaaaattct accatctctt
     2461 cagtggttct cattctatca ttacctatat catgattgtt ttgtgcagta tgatcagata
     2521 tgtttaattt tcttttttct gatggatcct tatctaaatc ttctaaattc aaagatctta
     2581 ttattttata aggagtagtc ataccgtaac cagctttttt catacaattg ccgcaaacaa
     2641 tttgtttatc tttgatttta tatgaagtta agccaatttt acctccgcac tttgcacatt
     2701 gtttactcac attagttcct cctagatatc agctttctac tactattaac attctatcaa
     2761 tagtgaccat actttgacca tactttttag aaagaataga aaattccaag atatttaata
     2821 atagaagaaa agcactaaca tcatgggttc agaagaattg agagcttatc aggaaacagt
     2881 ataattgaag tgtctgttta tccagtagct caagaagctt aattgaatct tatgattaaa
     2941 gaggttgtga caagactttt gtcacagcct tttttgttta taaaaat
//
LOCUS       DACBZN010000191         2954 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.196, whole genome shotgun sequence.
ACCESSION   DACBZN010000191 DACBZN010000000
VERSION     DACBZN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.196"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            89..406
                     /locus_tag="IVD64_002868"
     CDS             89..406
                     /locus_tag="IVD64_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6150069.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="IVD64_002869"
     CDS             complement(497..862)
                     /locus_tag="IVD64_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150070.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1069..1551
                     /locus_tag="IVD64_002870"
     CDS             1069..1551
                     /locus_tag="IVD64_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150071.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1748..2638
                     /gene="pstS"
                     /locus_tag="IVD64_002871"
     CDS             1748..2638
                     /gene="pstS"
                     /locus_tag="IVD64_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP6150072.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1009 a          460 c          566 g          919 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaagttttt ccccctaatt tttttagata
     1021 gaaaattgta tagtatgatg agagaaaaag tagtttgggg gaacgttaat gagaagaaaa
     1081 gcagtaatcg atcggcttta tttatttttt tggttttttc ctatcgtttt gctacctttt
     1141 acatgggtaa atgctgttgt acaaggtgcg gagatattag gcctgatcca aggaagcgga
     1201 tttgtggttt ctaggaattt tccagctttg attgttttct atttagtgat aagtgtaggt
     1261 ctcttattac caaaatctct gatcaatcac tacaattggc agctgcttta cttttcttgc
     1321 ttattagtat ttacatctac gttttctttg ctgcctcgct tgattatcgg ccctaatggt
     1381 ttgagcgaga gtggattgaa ggatttagct tattcctcca tgtatttctt gacgatcaaa
     1441 ccatggttct atttagtaat tgcctcattt attcttgcaa cattttttta cttctggaca
     1501 gaaatcagga taaaaagtga acgatctgta tctataaaaa ataactattg atggtggatc
     1561 atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt ctcatatgct ttttttatta
     1621 ttatgtaaag aatgcgaaaa acgtttacat gatttttaca taacttaact atatttttac
     1681 agcaaccata cgagggattt acagtcgact gataaacttt agttgtaaat aagaaaggag
     1741 caataaaatg aaaaaagtat tgtttactct aggtatgatg ggaattcttt tagccggatg
     1801 cggctcagga tcaggaaata cagatggctc tgccacacaa aataattcaa gtgactcaaa
     1861 tgaacaagta aaaatcgttg cggtaggttc gacagcatta cagccgttag tcgatgcagc
     1921 acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag
     1981 cggaactggt ttgagccaag tcgaagctgg agcagttact atcggaaact ctgatgtatt
     2041 tgcagaagaa agagacggag ttgatgcttc aaaattagtg gatcataaag tagcagtggt
     2101 cgggatggca ccaatcgtca ataaagatac agatgtcaaa gatatcacca aacaggaatt
     2161 gattgatatc tttacaggaa aaatcactaa ctggaaggaa gttggcggga aagatcaaaa
     2221 aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc gcgacattcg aaaaatgggg
     2281 actggatggt gctacccctg tgcagtccca agaacaagat tcatcaggaa cagttcggca
     2341 gctggtaagt caaacaccag gagcaatcag ctatttagct ttctcatatc tcgatgattc
     2401 cacacaagca ctaagtatcg acggtgtaga accaaaagaa gagaatgtag cagataacag
     2461 ctggggaatc tggtcatatg aacatatgta cacgaacgga aaaccttccc ctgaagtcca
     2521 aaaattctta gactacatga tgacagaaga aatccaagaa ggacctgtca aagaattggg
     2581 ctatttacca atcacaatga tggaagtaga acgtgatcac gaaggaaata tcaaataact
     2641 agcagataaa catgccacgg tagtcgttac tattaaggaa aaatcaacta tgtttcccca
     2701 catatacatt tttcctttaa ttgtgaaaaa tagttcctca tattttgccg ttttttgagc
     2761 taataccgag ggtcagctga ttgaacaccg tttattcgaa aaaaagagct acgacataga
     2821 actgtcgtaa ctttttttgg ctcttcgtca aatcgtgttg gtgaagacaa atttgaataa
     2881 aacttagacc gatatgcggc gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc
     2941 agaccttgtt gtaa
//
LOCUS       DACBZN010000192         2819 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.197, whole genome shotgun sequence.
ACCESSION   DACBZN010000192 DACBZN010000000
VERSION     DACBZN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.197"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            265..360
                     /locus_tag="IVD64_002872"
     CDS             265..360
                     /locus_tag="IVD64_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6150073.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(662..907)
                     /locus_tag="IVD64_002873"
     CDS             complement(662..907)
                     /locus_tag="IVD64_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150074.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1252..1536
                     /locus_tag="IVD64_002874"
     CDS             1252..1536
                     /locus_tag="IVD64_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150075.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1539..1955
                     /locus_tag="IVD64_002875"
     CDS             1539..1955
                     /locus_tag="IVD64_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150076.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2092..2811)
                     /locus_tag="IVD64_002876"
     CDS             complement(2092..2811)
                     /locus_tag="IVD64_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6150077.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
BASE COUNT          926 a          523 c          434 g          936 t
ORIGIN      
        1 tctcacattt tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg
       61 gactcctttg tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa
      121 actatgtcct tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa
      181 ggcaataaaa aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc
      241 cgacagtctt gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt
      301 cttgttgggc ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa
      361 aacttaatta aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg
      421 ctttcgtgtg tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat
      481 ttcaaaaata tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca
      541 aataaagtcg ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt
      601 gtatttcatt ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca
      661 cttaattata ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc
      721 cgtcttcgtt cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat
      781 ttttatattc caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct
      841 ctaatttttc taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca
      901 gtctcacaat ttttctagaa ataaaatcac ttctttgccg tctatactct caaaaaaaat
      961 gtcgtttgaa aaactaaata aatttttgat cgtgcatttt taaatttggt tatcgattac
     1021 acgtgcagca ctcccccccg aattttaaag agcataagga aacgtgtacc cactttctaa
     1081 aaaaagtggg tagataggct gtgcctatca cgtttctcag aaaaaaattt gatatcactt
     1141 tttcgccatc gtgggtagct tatgtatagc attttggcaa aaaacagcta tacaaatata
     1201 gtatttgtat gatatacata aattatacat aattgcaaaa ggagattttt tatggacatt
     1261 ttagttcgga atattgatcc aaagtttgtt gcaacaatag acaaacgttg ccaagaactc
     1321 acgaataaaa caggagaaaa atggagtcga aataaatatc taaaagtttt aatcgaaagt
     1381 gattttgacc gtcaacttac gacttacaaa aaagaccaat tcgatttatt gctcgataaa
     1441 tttattgcta tccaaacaga aaatactcaa atattaagcg agtatgtcta cacaaataat
     1501 caaatcattg aaatgcttat cggaggaatg atataaccat gagaaaaact tctgtccaaa
     1561 aacatgttac tttgtaccct agtcatgttg acgttttaga ttccatcatc gaaaaaaatc
     1621 cagatatcaa taactactcg gaagccattc gctttttatg tatgagttat gaacccgata
     1681 ccaacacgac cactaccagc aatcaggcaa aactaaatgc tatgggaaaa gaaatttcca
     1741 tcatgactga aatcatcgca gaatttgctg atttatatct aaaagataca ggcgttctta
     1801 ttggcacaaa ttctaaagta tatcaagaag tgaaagaaaa aatcgaagct aaactaaatc
     1861 catcaaatcg aaatcgacca aagaatcatc aaaaagaaac cagtcaaaca gtcacttcta
     1921 gaaatttcga cccaacaaat tttctaaaac gataaaaaac agataggagt tattcctatc
     1981 tgttttttat ttagaagccc catacaatat gctagaatgt actagcaatc ttagtcggtt
     2041 gtaagggact tgtctacttg cttaatgtaa ggggggcgag ttctttttta tttattcttc
     2101 taaccagtta cgcagagaaa tctttggcaa atctgacgtt aggcttgaaa aagtaaattc
     2161 taatttggcg attttattgc ccttttttgc ttttattttt gtgactttaa gatcattgaa
     2221 gtattttttt agttctcgta atgcaggatt taatactttt tgatcaatgt tacccatttg
     2281 atagtattct gggatatcca ttaagctcct aaactcttca atacgaacta cataataacc
     2341 agtagaacgg tactgcataa gcagtcggaa tagagttttt gcatacgtag aacgcaacga
     2401 cgtgaaagca gataattcaa atctactaaa ttcagctgtt aaaccattaa taatatattc
     2461 taggtccgga ttaacactga cttcaacaaa cttttttgtt ttattgattt caaaaccagt
     2521 aaacaaaaca aagtagcgaa tagaatcctc atcttcagta cggtaagtaa gattaagcat
     2581 atctttatag agcttttcta aatcaccgac aaaggctttt gtagcagtca tcttataatc
     2641 acttaactct tttaattcct cgaaagaaaa tctaactttt ttcaaaccac gatctttcat
     2701 acgagcgcat aatgcaaaaa ataaatccat ctgcttagca ttgaaatttt tcagcggtac
     2761 taaattcaat tcattttgat atttgactgt taattccgtt ttttcatcca ttcctttct
//
LOCUS       DACBZN010000193         2731 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.199, whole genome shotgun sequence.
ACCESSION   DACBZN010000193 DACBZN010000000
VERSION     DACBZN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.199"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            179..775
                     /locus_tag="IVD64_002877"
     CDS             179..775
                     /locus_tag="IVD64_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6150078.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IVD64_002878"
     CDS             899..2698
                     /locus_tag="IVD64_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6150079.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          776 a          468 c          516 g          971 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac a
//
LOCUS       DACBZN010000194         2635 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.200, whole genome shotgun sequence.
ACCESSION   DACBZN010000194 DACBZN010000000
VERSION     DACBZN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.200"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            105..1304
                     /locus_tag="IVD64_002879"
     CDS             105..1304
                     /locus_tag="IVD64_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6150080.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1331..2599)
                     /locus_tag="IVD64_002880"
     CDS             complement(1331..2599)
                     /locus_tag="IVD64_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP6150081.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT          788 a          573 c          468 g          806 t
ORIGIN      
        1 aaacatcttt ccatcggaaa gatgtttttt tgctttttct agtttaaata acagcttttt
       61 tcatctattt tcgttatact gtataagaaa aaggagttga atttatgaat ctaaccagag
      121 atctgatctt tttagttctc tatcttattg gggtattctc tatcgtttat tggcgaaaac
      181 agaagaagga tccttccttg ttcttttacg gcagtacgtg gacatttacg gtaatcttaa
      241 cttattttgt cgtgttggaa attttttatt cgacctttta ttttttgatt ccattgttgt
      301 tctttagtat ctttgctttt tcttatttca cagaaaaacg aaggctactg aatggtcttt
      361 tattcaatgt tttactcatc agtttcggca tctacttgtt tgtcttgctg tatgaaacgc
      421 aaaatatttt tcttggcgga ctgatcgcct tgatcacgat tccattgcta cttgtccttt
      481 tatttggtat ttatggattg attgtttttc ttttttggaa cggggtcaca gttttacgta
      541 gagaatcgca ttcattagct aatcttttaa cgttgatact agccattttt cttacactgt
      601 ttcttgtttt tgactttttc ttgttaaaat atttgccgca atggataaat gctttgtttt
      661 tctgcgttcc cctgatcttg atctatctat ttatcgtatt ctacaacttt ttgactgtct
      721 cattcttgta tcagttcaac cggcctcgat acaatcaaga cttcatcgtt gtgttaggcg
      781 ctggattgat caatggagaa acagtttctc ctcttctagc caagcgaatc aataaggcaa
      841 ttgcttttta tcgggctcaa agccgtgcta cattaaatcc gcccatctta ttgatgtctg
      901 gcgggcaagg agcagacgaa aaagtaccag aggccattgc gatgaaacaa tatgccatgg
      961 aacaaggaat ccctgaaaga gacattcttg tcgaaaccaa ttcgacaacc actttggaaa
     1021 acatgctcta ttcaaaggaa atcatggatc agcaaatgaa agggccatac cgcgcgatct
     1081 ttagttccaa caattaccac atttttagag ccggtcttta tgcgcggcaa gccaaattga
     1141 aagcaaatgg tattggtgca aaaacagctt tctactactt gcctaatgcg tttttaagag
     1201 aatacctcgc catcttgatg atgaacaaaa aaagacatat gatcatttgc ggattgcttt
     1261 tcgcaggctc cgcattctta tcgctgatta ctttactttt ctaaaaaaac aaggtgtgac
     1321 aaaacatatg tcacaccttg tttttttctt tcattctctt aactattggc aaaaagagta
     1381 tccctttttt gaccgctact tgctgagcag aataaatacc gctgttgcct gaacacataa
     1441 agctgatcaa gcagatcata aagaagtata ctgccgcttg tgaaccgaac aattcgatcc
     1501 ccatcacaaa gcaggcaatc ggtgtatttg tcgcaccaga aaatactccg ataaagccca
     1561 atcctgcaag aaaaggaaca gaaacatgga gcaaaggcgc tagtgctgca ccaagcgttg
     1621 caccaatttc aaacaatggt gtcacttctc ccccttgata tcccgcgcct aaagacaaaa
     1681 ctgtgaagaa taacttccca ataaaatcat agacatgtgc ctgtccatta aatgattctt
     1741 gaagtaacgg aagactcaat ccgagataac gttgtgtttg caaaatcaat gctgctgcca
     1801 caactacagc tccacctatc gtatttcgac aaacaggatg aggaatccat tgtgcatatc
     1861 gcgcttttaa aaagacgatc gaacggctga atacccaacc aatcaaacca aaagcaatcc
     1921 cagcaacaaa aactttgaag aacagcaaca tactccattc aggaatcaca cccatttggt
     1981 aatgtagatg agaaacacca tagctttctg tcatgaaatt tgcaaacagt gcagcaaaga
     2041 agctagggaa gatcgcttct gttcggactt tcccgattgc caacacttct aatccaaata
     2101 atgttccagc taaaggtgtg ccaaatacag aactaaaacc cgcactgatt ccactgataa
     2161 tcaagatttc ccgttctgat ttatccaatc ggaacaaacg tgccgtatta tctgctaacg
     2221 ctccccccat ttggactgct gttccttcac ggccaacaga tcccccaaat aaatgggtag
     2281 ttatggttcc aaacagtgta agtggaataa gccgcaacgg gattttttct tctccaccat
     2341 ttccttgatc gatcactaag ttgtttccac gactagaaag tccgccgtga tacgcataga
     2401 gataagcaaa aagcgcacca ctaactggta gtagacaaag gagccaagga tgtgatagtc
     2461 ggatatctgt cacaactgtc aggctattta agaaaaaagc agacaagctc cccatgaata
     2521 taccgagaat cgctgtgatc accagccact tcagcatata cactaaaatg cgtactggac
     2581 tcggtaattg tttttccata aaaaattcct cctcaataaa aaaacgccta ctaaa
//
LOCUS       DACBZN010000195         2532 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.202, whole genome shotgun sequence.
ACCESSION   DACBZN010000195 DACBZN010000000
VERSION     DACBZN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.202"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..802
                     /locus_tag="IVD64_002881"
     CDS             <1..802
                     /locus_tag="IVD64_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6150082.1"
                     /translation="NAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHT
                     HDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYG
                     YLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQF
                     GEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVG
                     YHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            917..2125
                     /locus_tag="IVD64_002882"
     CDS             917..2125
                     /locus_tag="IVD64_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150083.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
BASE COUNT          930 a          369 c          450 g          783 t
ORIGIN      
        1 caatgcctat acagcgtata ttgttggcga tggtgggatc gtttaccaag ttggacaacc
       61 tggttatgta caatatggcg ctggttcata tgctaatgca aatagtcctg tgcaaatcga
      121 actgcaacat acgcacgata aagtaacgtt tgaaaagaac tacaaggcat atgttgaatt
      181 agcgagagat tccgctaata aatacggtat tccacttaca ttagacacac cgtataatca
      241 accaggaatc aaatcacatt tatgggtaac tcaaaacatt tggggcgatc acactgatcc
      301 gtatggatac ttatcagaaa tgggtgtaag taaggaaaaa ctggcttacg atttagctca
      361 tggatttaca gacgaaaacc caacgacttc tgagaacaag cctgtcattg atccaacccg
      421 agcaggtgca gcaaatccaa ctttatcaga tggaacgaac cattctcaca ttgatcagtt
      481 tggagaaatc gaaaatgcga acttacacgt cgctggatgg cacatcgcta actataaata
      541 cgagtatatt ttcattatgg actacaatac tgggaaagaa ttagctcgag taagagctga
      601 tggaatttat agaccggatg taaatcaagc ttataatact tctggaaacg ttggctatca
      661 tgtatctttt aacatgcgca atttccctaa taagaaagta tacgtcatga tgcgggcaac
      721 gaatgatcca gaaggaaaca ctaaaggcgg tgcgcaagat ttccatgaca aacgttggta
      781 tttaaatatt cctaaacgat aaaaatagct cctcgttgag gagcagtaca tagaattgaa
      841 aattaacgtt aaatcaaaaa aatatttaca agatgtttat ttaagtagat aataaaagaa
      901 aaaagcaggt gaaattgtga cagcagaaat tggtattatg aataaaagtg gaatcgtgtt
      961 agcttcagat tcagcatcta cgattggaga tagcaaagta tataatactg caaaaaaatt
     1021 atttactttg gattccatgc attctgtagg tatcatgata tatggtaatg ctgaatttaa
     1081 tggtattcct tgggaaataa tcattactca atataagaaa agcattggta gttctgtttt
     1141 taatactttg gaagaatacg cggataattt tatagaattt gtcaaaacag catcttttat
     1201 tagaagtgaa cagaccgagc aagaacaaat gattggggtt tttcaaaaaa taatttcagg
     1261 tttatttgag agtatagaag tagatattaa ctttttaata agccaaggta cacaaataga
     1321 caaagatgtc ctagtgaagt tattacaaac aaaaatgaat accaatttgt ctcaacagtc
     1381 ccagacattc attttagata ttgaaaaggc gttatttctt agtaactatg gagaaatatt
     1441 aaaagatatt cttaatagta tttctacaat ggaaggtgtt tcagaagcta tatcagagga
     1501 aattcaaagt tacgtttatg aaattataat tagagacgat gtttattctt cccctacagg
     1561 aatagttata gcaggatatg gaagaatgga tattttccct aaactatatt cttataatat
     1621 gtttggattt gttatgaata ttctgaaata ctcagaatat gaaagtgctc aaataggaaa
     1681 tgacaatggt tcattaagat ctactatatt accatttgct cagtctgatg tggtaaatac
     1741 tgtagttcaa ggggtagatc cacaaattac taattatctg tcgagtcaag tcgatagttt
     1801 tgacgataac ggaaaaaata cctatattaa tattataaag aatatttcag agtttcaaca
     1861 aaatcaattc atatttccgt tactaaacat gattgcttta ctcccagttg aagaaacagc
     1921 aattatcgca gagactttat taaatttaac cagttttaaa cgaaaatata ctacttctgt
     1981 agaaacagta ggtggcccaa ttgatgtttt agctattaca cctaatgatg gccctatttg
     2041 gattaaaaga aaacattatt ttgatattga taataatatt ggatacagat tgcgaaagga
     2101 gagagcgaat gattacaata attaaacctc aggaaaaatc ccctgttttg aaatttaatg
     2161 agagcaatct agcccataat aaaatgagta taactgatac agtatttgat gtaatggttg
     2221 agcaacaaaa aaaaattaat aataacaata gacaaagaaa agaaattaac caatattaat
     2281 gttttttctg ttgcgttttt tgcagtcata atttaaaacc tcgctcaatc ttgggcgagg
     2341 tcttttttta tttttctgaa aagttaaagt aaataatagc ataaaataat atattttaca
     2401 aagaataagt acaatctagt tttttgctat taaatgtgta ataataaata tgccatcaca
     2461 acatgaagaa tgaaatccat tattatctag tctagtccat tctttttgtt tgcagtattt
     2521 gtgatggctt tc
//
LOCUS       DACBZN010000196         2484 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.203, whole genome shotgun sequence.
ACCESSION   DACBZN010000196 DACBZN010000000
VERSION     DACBZN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.203"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            2..1147
                     /locus_tag="IVD64_002883"
     CDS             2..1147
                     /locus_tag="IVD64_002883"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP6150084.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="IVD64_002884"
     CDS             1318..1776
                     /locus_tag="IVD64_002884"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP6150085.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="IVD64_002885"
     CDS             1935..2405
                     /locus_tag="IVD64_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP6150086.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          825 a          469 c          467 g          723 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaacttaa taaaaataaa aaaagatgaa ctatttctag ttcatctttt tgcctttgcg
     2461 tggcgacgtc ctactctcac aagg
//
LOCUS       DACBZN010000197         2483 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.204, whole genome shotgun sequence.
ACCESSION   DACBZN010000197 DACBZN010000000
VERSION     DACBZN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.204"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(1014..1304)
                     /locus_tag="IVD64_002886"
     CDS             complement(1014..1304)
                     /locus_tag="IVD64_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150087.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IVD64_002887"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IVD64_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACBZN010000198         2397 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.205, whole genome shotgun sequence.
ACCESSION   DACBZN010000198 DACBZN010000000
VERSION     DACBZN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.205"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            206..2068
                     /locus_tag="IVD64_002888"
     CDS             206..2068
                     /locus_tag="IVD64_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6150088.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IVD64_002889"
     CDS             2068..>2397
                     /locus_tag="IVD64_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6150089.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACBZN010000199         2397 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.206, whole genome shotgun sequence.
ACCESSION   DACBZN010000199 DACBZN010000000
VERSION     DACBZN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.206"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..94)
                     /locus_tag="IVD64_002890"
     CDS             complement(<1..94)
                     /locus_tag="IVD64_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6150090.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREV"
     gene            complement(168..449)
                     /locus_tag="IVD64_002891"
                     /pseudo
     CDS             complement(168..449)
                     /locus_tag="IVD64_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            911..2293
                     /locus_tag="IVD64_002892"
                     /pseudo
     CDS             911..2293
                     /locus_tag="IVD64_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          872 a          409 c          400 g          716 t
ORIGIN      
        1 gaacttcacg atagcttaga ttgtaacgca ggtagtaacc aacagcgaca ataatgacgt
       61 cttttttgaa ttgtttgcct ttaaaatgat tcattactct gtcctctctg tcttttttct
      121 caattttaca ctaaaataga ttttttggaa aactttgcaa cagaaccgaa atgagtatag
      181 tatgaattag taatttggtt ttcatctaag tttagctttg atttactaaa attgatatct
      241 cgatacatgt tcaatacatc taatacaaat cgagaatctt ctttcggaaa tggctcagaa
      301 agatcactcc aaatttcatc atcataatga tactcgtaac cttctctcaa tatttcgatt
      361 tttctttcat aataagatct gttttggtcc tcaaatttag ccaataactc aaattgattt
      421 actagtgaca atctctgaaa actgtccatt aaatgtttct tcctatcttg ttcattaaca
      481 aaaaattata agcccttaca acgaatatta tatattaaat ttaattgtag gtgaacacga
      541 attttataaa tatttatggt gttaatgaca aatgatatcc gactcctctt catacgttct
      601 ggactatttg ctaatagatt aaaatttcag catctatttc tgaccataaa taaatgagct
      661 gccgaaaagc tgccaaatca gacttgaaaa taaaggatca aaaactttta agtttctgac
      721 cccgtaactc acacttcaaa atcttactta aaaaggagtc tgaaagaaaa aaagagtatt
      781 caaaattttt ttctaactag ctacatcaca tctatgcatt gacactcaaa gaaccagaat
      841 aagtcatcag aaagcaaggg ggtgcggaca taatcctgga cgaaaaaata aacttaggag
      901 gaatgtccgt gtgttttccc acaaagagcg tgtaagggca attcagttat ttttaaaata
      961 cgattgctct tatgctgcaa ccattcgaga attagggtat ccgtcgattg gtgcactacg
     1021 gaaatggtat aaagagtatt tggaatccgg agagctccat caggagcacc gaaaaaaatc
     1081 aaagtattct gaagaacaaa aacgaatagc ggtgaatcat tattttgaat atggtcaatg
     1141 ttatgcacga accattcgca tgcttggtta tcctaataga gagtccttgc ggcagcggtg
     1201 tgaagaactt gcaccaggag ctagaaaact ccgaaaaagt gcggtaaagt taacacaaga
     1261 tcaaaaagaa gctgtgttga aaaaattcta taaaccgcaa actaatagga aaaatttagc
     1321 cgaatctgga ggaattagta gagttactct atatcaatgg aaaaacacgg ttcttggaaa
     1381 ggattttcca ttgtgtatga ctttaaaaaa tcaggaaaaa caaaaagagc tacttttaaa
     1441 ggaagtagaa gacttacaaa aacaagtaca ccaattacag ctcgaaaaag cattactcga
     1501 aggagcggct gaactgctaa aaaaagaaaa aggcgtcaat cttctttgtc tatcaaatca
     1561 agaaaaaacg atattgattg atgccttacg aaatcagttc accttgaaag agcttcttca
     1621 acagctccag tttcctaaaa gcagctattt ttatcaaaaa caagcacttg agaaaccaga
     1681 taagtattac aaagaacgtc aaattaatta tcacgatttt caatcataac ttctgtgcgt
     1741 atggttaccg tagaattcac caagctttaa aaaatatggg taaaaagctt tctgaaaaag
     1801 ttgtacgtag attgatgact gaggaaaatc tatttgttaa attttctaga cgaaaaaagt
     1861 attcttcgta tgctggggaa atttctcctg cacaccccaa tttattaaat cggaatttta
     1921 aagcaaagaa gccaaatgaa aaatggttaa cagatattac tgaatttaga attctcgcag
     1981 ggaaaatcta cttatcacca cttgtcgatt gttacgatgg tgctattgtt agttggacta
     2041 ttggaacaaa gccaacttgc ttcactctat gtctaaaaag agttattcac cagataattc
     2101 agcgtgcgaa ggtttctttg gacgattaaa aaacgagatg ttttatcaac gtgactggaa
     2161 gaatacaaca atcaatcagt ttataaacaa attggatgat tatattcatt ggtataataa
     2221 tcaacgaatt aagttatctt taggaaggct aagccctctt caatatcgga agaagcaaag
     2281 atatatttgt taaaaaggtc caaaaaaaat atccgcaccc cctttttttt gtaaaaaact
     2341 attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga gcaagaa
//
LOCUS       DACBZN010000200         2395 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.207, whole genome shotgun sequence.
ACCESSION   DACBZN010000200 DACBZN010000000
VERSION     DACBZN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.207"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            316..570
                     /locus_tag="IVD64_002893"
     CDS             316..570
                     /locus_tag="IVD64_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6150091.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IVD64_002894"
     CDS             571..888
                     /locus_tag="IVD64_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6150092.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="IVD64_002895"
     CDS             1447..2382
                     /locus_tag="IVD64_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150093.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          340 c          453 g          741 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat tcttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DACBZN010000201         2164 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.209, whole genome shotgun sequence.
ACCESSION   DACBZN010000201 DACBZN010000000
VERSION     DACBZN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.209"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..111
                     /locus_tag="IVD64_002896"
     CDS             <1..111
                     /locus_tag="IVD64_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6150094.1"
                     /translation="YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            325..1020
                     /gene="vanR"
                     /locus_tag="IVD64_002897"
     CDS             325..1020
                     /gene="vanR"
                     /locus_tag="IVD64_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6150095.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            998..2152
                     /locus_tag="IVD64_002898"
     CDS             998..2152
                     /locus_tag="IVD64_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6150096.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          730 a          400 c          461 g          573 t
ORIGIN      
        1 tatgcggtaa agctatataa agaaggaaat atgactgtaa atcaaatttg tgaaattact
       61 aatgtatcta gggcttcatt atacaggaaa ttatcagaag tgaataatta gccattctgt
      121 attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa ctataaaata ttaacagcct
      181 cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca tcatcttaag aaattcttag
      241 tcatttatta tgtaaatgct tataaattcg gccctataat ctgataaatt attaagggca
      301 aacttatgtg aaagggtgat aactatgagc gataaaatac ttattgtgga tgatgaacat
      361 gaaattgccg atttggttga attatactta aaaaacgaga attatacggt tttcaaatac
      421 tataccgcca aagaagcatt ggaatgtata gacaagtctg agattgacct tgccatattg
      481 gacatcatgc ttcccggcac aagcggcctt actatctgtc aaaaaataag ggacaagcac
      541 acctatccga ttatcatgct gaccgggaaa gatacagagg tagataaaat tacagggtta
      601 acaatcggcg cggatgatta tataacgaag ccctttcgcc cactggagtt aattgctcgg
      661 gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag taaaggagca gaacgaaaat
      721 gttatcgtcc actccggcct tgtcattaat gttaacaccc atgagtgtta tctgaacgag
      781 aagcagttat cccttactcc caccgagttt tcaatactgc gaatcctctg tgaaaacaag
      841 gggaatgtgg ttagctccga gctgctattt catgagatat ggggcgacga atatttcagc
      901 aagagcaaca acaccatcac cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc
      961 attgataatc cgaaatatat aaaaacggta tggggggttg gttataaaat tgaaaaataa
     1021 aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt
     1081 ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg
     1141 gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata
     1201 tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat
     1261 tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac
     1321 cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga
     1381 tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc
     1441 aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac
     1501 gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt
     1561 agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct
     1621 aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac
     1681 gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc
     1741 cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc
     1801 tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga
     1861 taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa
     1921 gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct
     1981 ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga
     2041 aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt
     2101 tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta
     2161 tata
//
LOCUS       DACBZN010000202         2162 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.210, whole genome shotgun sequence.
ACCESSION   DACBZN010000202 DACBZN010000000
VERSION     DACBZN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.210"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            147..1235
                     /locus_tag="IVD64_002899"
     CDS             147..1235
                     /locus_tag="IVD64_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6150097.1"
                     /translation="MKEERKLPEWLIDYAEKEGLIAELKPKHERQNFLVGDDRLDHAV
                     AFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKF
                     FESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEIC
                     VDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVE
                     PEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKV
                     CERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQ
                     LKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1246..>2162
                     /locus_tag="IVD64_002900"
     CDS             1246..>2162
                     /locus_tag="IVD64_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6150098.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASA"
BASE COUNT          793 a          380 c          461 g          528 t
ORIGIN      
        1 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
       61 gagtggaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt gattatcaac
      121 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga
      181 ttgattatgc agaaaaagaa gggttgattg ctgaactaaa gccgaaacat gaacggcaaa
      241 attttttagt tggagacgac cgtttagatc atgcggttgc ttttttatgg aaagatccac
      301 agacaggaga gacagtaggg gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg
      361 gtaaacgggg aacgtataaa cacattgaca agaatcctac gccaaatcac ggatttaatt
      421 taaaaatagg cgatccgaag catttaaaat tctttgaaag tagtattgat ctgttaagtt
      481 acgctgcatt gaatcgggag aaactacaag atgcctggtt agtttcgatg gacgggttaa
      541 aacatcatgt aatcagtcat tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa
      601 catttcctca atcgattgaa atatgtgtgg acaatgatcg tgcaggacat attttttatg
      661 aaaaagaaca gatgaaggga atcgttgatc cttttaccaa taagaaaatc cgttgtgagc
      721 gaggaattcc aaatgattgg caagtaccta aagaatacaa agctacttat gaagcagtcg
      781 caaaagaaat gagtgttgaa cctgaagcaa ttatggccat acacaaaact gaaactaatt
      841 tacagctgac aaatcaattg gtgtctgctc atgatgttca atctacgttt ggaaagatgt
      901 tggcgaaggg cgaaccagta gaaacaattg atttgaaaga agcatgtacc acggtcgcga
      961 aggaactgaa agtatgtgaa cgcgcagatg gaacatacaa ctttgatcgt ttttatagta
     1021 gaaaagccaa cataaaagac gttaatgcag gaatccttct ttcctataaa gcagaacaat
     1081 actacaaagg ctataaaaag catgaacatg aatttgtacc tgaagtgaaa aaggattgga
     1141 atgatcaatt gaagcatgag atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt
     1201 tgtttcaaca aggcagacaa caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt
     1261 ttagcagaaa aaccgtcaca agcgaaggct tatgccgata gcttttctaa agctacccga
     1321 aaagatgggt attttgagat ccaagatcga ttgtttacag gagaaactgt gatcacgtat
     1381 ggttttggtc atttagtcga gttagattcg cccgatatgt acgatgaaaa atggaaacaa
     1441 tgggcattag aacatttgcc tattttcccc aatcaatacc actatcatgt tcctaaagat
     1501 aagaaaaagc agtttagtgt cgtcaaacga cagctgcagt cagctgatac gattgtgatc
     1561 gcgactgata gcgatcgtga aggggagctg attgcctggt caatcattca acaagcaggc
     1621 gccaatcaag gaaaaacctt taaaagactt tggatcaact ccttagaaaa agaagccatt
     1681 tatcagggat tccaacagtt acgagacgca gaagagacct atccaaaatt tgaagaagta
     1741 caagcacgtc agatcgccga ttggttaatt gggatgaacg gtagcccact gtatagttta
     1801 ctcttgcagc aaaaaggcat accaggaagt ttttctttag gtagagtcca aaccccaaca
     1861 ctctatatga tttatcaatt gcaggaaaag attagaaact tccaaaaaga accatatttt
     1921 gaaggaaaag cccaagtaat cgctcaaaat ggcgcatttg atgcgaagct tgatccaaat
     1981 gaaacccaag cgacacaaga agcatttgaa gcctatttaa aagaaaaagg tgttcaatta
     2041 ggaaaacagc caggtacgat ccttcaagtc gaaacagaga aaaaaagtgc ggctagccca
     2101 cgtctttttt ctttatcgag ccttcagtct aaaatgaatc aactcatgaa agctagtgcc
     2161 aa
//
LOCUS       DACBZN010000203         2082 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.211, whole genome shotgun sequence.
ACCESSION   DACBZN010000203 DACBZN010000000
VERSION     DACBZN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.211"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..375
                     /locus_tag="IVD64_002901"
     CDS             <1..375
                     /locus_tag="IVD64_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6150099.1"
                     /translation="IFKGANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFS
                     DIQKLSLGEVMDLVSNDGVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLL
                     TLFTCVYTSQGKERYVVQGELQ"
     gene            428..>2082
                     /locus_tag="IVD64_002902"
     CDS             428..>2082
                     /locus_tag="IVD64_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6150100.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAV"
BASE COUNT          750 a          319 c          466 g          547 t
ORIGIN      
        1 atttttaaag gggcaaatca atacactttg agtcttggag cggctactta tttttatgaa
       61 gatgccgaaa tgggcaaagg aaactatgtc ctagcaggtc ataacatgga aatgccaggt
      121 gttttatttt ctgatattca aaagttgtca ttaggggagg tgatggactt agttagtaac
      181 gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg tacctgggta ctttaaatta
      241 atcgatggcg ttcctgaaga gaactctttc ttgtctttgc caaagaaagg agagaaaccc
      301 ttattaacgt tgtttacttg tgtttatacg tcacaaggaa aagaaagata cgtggtacag
      361 ggagagctac aataacaagg ttttaatcaa cgcgagtcgt tgaaatatga aatgaggaga
      421 gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga atgcttgttg gaatgttagg
      481 aatcattgca ggctgctttc aggggtatca agtacatgca gaagatgtca cgcaaaaaac
      541 accaccagag aaggtcaata tcacggttca taaattaatg tacgatcaag gaacgcaatt
      601 gaatgttgat attgacggga tcaaaaacga tggttatacg cacgatcaat atcctacagg
      661 tgtaacgaaa tacaacaagg cagattatgg tgatgttgag tttacgttag gaaatattac
      721 ggatcaagta ttaccgagag aagattctga tctaaccaac gcgaaagtgg acgagattgt
      781 acaagatgta gaagacaagg gatctaacag tgagtacgtc aagaatgcaa ccaacaaaat
      841 tacttcagct gtagatgaaa atggtgagat cacttttact gatcaaccag cctatgtcaa
      901 ttccaaaggg aatgtatatg tggtttatga aagtaaatcc gctgctggat tagtcacaca
      961 aaaagcaaaa ccaatggttg tgattgcgcc aatgacagat aaaacaggat caggattttt
     1021 aaaaaatatt catttgtatc ctaagaacat tgtttctaag ttgagttttg aattaacgaa
     1081 atttggtgac gatggcgcag accaaagcaa acaaaccctt ttaaaagggg caaaatttga
     1141 gttatacaag ggcgaacctg gaaaaggaac aaaattgggt gatcttgtat cggatgatca
     1201 aggaaagctg actgcgactg atttgacgtt aggaaaatac tattttgtgg aagttccttc
     1261 ggaagttgta gtgggatcag atcaagaacc aacagcagat caatatttat taggcgcaga
     1321 cgctcgcaac gatgcgcaca ataaattaac ctttgaaatt acgaatgatg gcgtgacaag
     1381 tgacttaaaa gcttcatacg taaactataa agcaccagtg atcgataaaa cagtaacaaa
     1441 tggaacagga caagaacatt ctttccaaat tggtgacgca gtaaattatc aaggaacgat
     1501 tcatattcca acagacattg caggtggcgc agacggtatc acagtaaacg gtgtgaaaag
     1561 cgaaacttct ccttacagtg tatttaaatg gggagataca gcaggtaaag gactttctta
     1621 tgttgctgaa aaagcagata ttaaagtaac aaataatgat ggttcagtcg tattaaaaga
     1681 aaatactgac tataaaatcg aaaattctga tagtggtttt gtgatcgatt ttatcgtaaa
     1741 taatggacaa gtaagtgata cagttgcgaa tttacatggt caagatctaa aaatgactta
     1801 taacatgttt gtgaatgata gtgctgaaat cgcaaatcca ttaacaaata gcgtggattt
     1861 cgtttataac aataatccat ttaatcagga agaacaccac gaaaaaacga aagctgacgt
     1921 tgtgacttat ggcgcaaaat tcttaaaagt cgatagcggc ctatttggaa caggtatcaa
     1981 agcaacaccg cttgaaggcg cagaatttgc agctaagaat gcagaaggta agtactacgg
     2041 tggacttact gatacagata aagatggtgt caaagaagca gt
//
LOCUS       DACBZN010000204         2081 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.212, whole genome shotgun sequence.
ACCESSION   DACBZN010000204 DACBZN010000000
VERSION     DACBZN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.212"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..375
                     /locus_tag="IVD64_002903"
     CDS             <1..375
                     /locus_tag="IVD64_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6150101.1"
                     /translation="IFKGANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFS
                     DIQKLSLGEVMDLVSNDGVYRYKVTRKFIVPGYFRLIDGVPEENSFLSLPKKGEKPLL
                     TLFTCVYTSQGKERYVVQGELQ"
     gene            427..>2081
                     /locus_tag="IVD64_002904"
     CDS             427..>2081
                     /locus_tag="IVD64_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6150102.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQVGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAV"
BASE COUNT          744 a          337 c          464 g          536 t
ORIGIN      
        1 atttttaaag gggcaaatca atacactttg agtcttggag cggctactta tttttatgaa
       61 gatgctgaaa tgggcaaagg aaactatgtc ctagcaggtc ataacatgga aatgccaggt
      121 gttttatttt ctgatattca aaagttgtca ttaggggagg tgatggactt agttagtaac
      181 gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg tacctgggta ctttagatta
      241 atcgatggcg ttcctgaaga gaactctttc ttgtctttgc caaagaaagg agagaaaccc
      301 ttattaacgt tgtttacttg tgtctatacg tcacaaggaa aagaaagata cgtggtacag
      361 ggagagctac aataacaagg ttttaatcaa cgcgagtcgt tgaaatatga aatgaggaga
      421 gtaaaaatgg caataaatcg atatgtaaaa aaagctggaa tgcttgttgg aatgttagga
      481 atcattgcaa gctgctttca ggggtatcaa gcacatgcag aagatgtcac gcaaaaaacg
      541 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaaag tacgcaattg
      601 aatgttgata ttgacgggat caaaaatgat ggttatacgc acgatcagta tcctacaggt
      661 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
      721 gatcaagtat taccgactga agattctgat ctaaccaacg cgaaagtgga cgaaatcgtg
      781 aaagacgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caataaaatt
      841 acttcagctg tagatgaaaa tggtgagatt acttttgcgg atcaaccagc gtacgtgaac
      901 tcaaaaggaa atgtgtatgt tatttacgag agtaagtccg cagcaggatt agtaactcaa
      961 aaagcaaaac caatggtggt cattgctcca atgactgaca acacaggttc aggtttttta
     1021 aaagacattc acttatatcc taaaaatatt gtttctaagt tgagttttga attaaccaaa
     1081 ttcggtgatg atggtacaga gcaaagcaaa caaacccctc ttaaaggcgc taaattcgaa
     1141 ttgtataaag gagaacctgg aaaaggaagc aaactaggcg atcttgtttc ggatgatcaa
     1201 ggaaagctga ctgctactga tttgacgtta ggaaaatact attttgtgga agttccttca
     1261 gaagtcgtag tgggatcaga taaagaacca acagccgatc aatatttatt aggcgcagac
     1321 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     1381 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     1441 ggaacaggac aagaacattc tttccaagtt ggtgatgccg ttaattatca aggaacgatt
     1501 catatcccaa cagacattgc aggcggagca gacggtatca cagtaaacgg cgtgaaaagc
     1561 gaaacttctc cttacagtgt atttaaatgg ggggatacag caggacaagg actttcttat
     1621 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatcagtggt attaaaagaa
     1681 aatacagatt ataagatcca aaattcagaa aatggctttg tgatcgattt tatcgtaaat
     1741 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     1801 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     1861 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     1921 gtgacttatg gcgcgaaatt cttaaaagtc gatagcggac tatttggaac aggaattaag
     1981 gcaactcctc ttgaaggcgc agaatttgca gctaagaatg cagaaggtaa gtactacggt
     2041 ggacttactg atacagataa agatggtgtc aaagaagcag t
//
LOCUS       DACBZN010000205         2061 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.213, whole genome shotgun sequence.
ACCESSION   DACBZN010000205 DACBZN010000000
VERSION     DACBZN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.213"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            189..596
                     /locus_tag="IVD64_002905"
     CDS             189..596
                     /locus_tag="IVD64_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150103.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENYWIGEEKLNEIY"
     gene            583..1035
                     /locus_tag="IVD64_002906"
     CDS             583..1035
                     /locus_tag="IVD64_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150104.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            1028..1426
                     /locus_tag="IVD64_002907"
     CDS             1028..1426
                     /locus_tag="IVD64_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150105.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            1738..1811
                     /locus_tag="IVD64_002908"
     tRNA            1738..1811
                     /locus_tag="IVD64_002908"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1772..1774,aa:Ile,seq:cat)
BASE COUNT          755 a          284 c          361 g          661 t
ORIGIN      
        1 atcaaacaaa aagtatacat ctttcttcat tctagttttt gcagcaggga ttttaaagta
       61 gttcttatta gaatagtaga gattgattaa taagctatct tcgattgcta gaaagaaaac
      121 ttctgaatcc cacaccttat aaaaatcttt gataaatcta ttcgaaggat caaatttaaa
      181 ccataattgt gtctttcctt ctattaaaat atcttgaaat ttactctgtt aaaaaaaata
      241 aattaattgg tgaaaatgat tctccaataa aaacacctat tgagctaagc ttaagaaatc
      301 ggatgggaat actccaaaga tcggaaatag taaaagaact tttttatcat aaatttagtt
      361 atgaaataag actagatgat acttatgagc attatagaat tgtttttttc gtatacaatt
      421 ctatagaaga tgctactgcg actactgcat taacattcgg ttttacgaaa aatggaacaa
      481 tcaattcgga taaaacccgt caagctgcta cagaaagtga tgatatatgt aagaaagtat
      541 gcaatgggga agagaattac tggatagggg aggaaaagtt gaatgaaatc tattaatgat
      601 ttattaaaaa aacatcctga tttatataaa aaattaaatt ctgaacaaag aaacctgtcc
      661 aaacaattac ttaaaataag attaaaaaaa caaaaaaacg tgacagaatt ctcaaaaatg
      721 ttaggaataa gtgaaaaaaa atatcttgaa tttgagttcg gagatatgca tattcctgtt
      781 gcagagtaca ataaactttt gaaacaagtt gaaaatttgt cctataaaga ttcaatgaat
      841 ttcactatta aggttgctgg cggccctatt aaatatgacc gtattgaaaa taaaccttat
      901 attcggaata aaagttactc agtatttaaa agtagaaaga gaaaagaagt atcgtcaaat
      961 atggtctatg ctagtgttaa ttaccaagat acgagccgct atattgtaga aaaagaggta
     1021 tatgtacatg gataaaggaa atagtgttac ttttgcactt gaaaatatta gtttgaataa
     1081 aattcatttt gaattaactg ataattttag ttatggagat ttagagttta atattgaact
     1141 tggatataaa gaagacgaag cagagtatct agaaaaaaaa cttgtttctt tacgagctca
     1201 cattgtaggg gggatggaag gagcaccggt agatatttta ctcttttcag ttattcgtgt
     1261 ttctacaaat gaacagccat ataaaaaatt agtagaagaa tctatgggag agctagctaa
     1321 acctatcctg aataaagctt ctaatttgat ttctaattta tatagcgaag taggagttat
     1381 gcctacagtt attgatttat atcagctata tagtcaacaa aaataaaata caagtaatag
     1441 agttatctat ttaaatttgc aagacaaaga cctcaaccag tcatccttgg ttgaggcttt
     1501 tttgctctgt aattaactcg tatagatcct gcaagatagt ttgtttctga ataaatgatt
     1561 gatatgaagt atttagatga ctgtattgac aactataaaa atcattcgat aaaatagtga
     1621 tgttatcgca tatcttcact atcacccata atagtcacac tccaagctat gcgataacag
     1681 gtttgttgcc acacattcta ctggttgatt gtttatggct ttatgtggca acaaccagta
     1741 cccttagctt agttggtcag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
     1801 tacagggtac ataatttaat ctatttgata aaaaataaaa ttcaataatg gtataataaa
     1861 atatcggctc gtcgcttcca cagagatttt cgtgggagga ggtgtatgat ttgtttgaat
     1921 tcactttgga gcacgtgctc ttgccattgt taattggttt aactttggca ttatttagtc
     1981 attggctaga taaacattag agccgacaat ggcgcttact gtttcgcagc ggtaagcgtc
     2041 tttttttgaa aaagaaaaaa a
//
LOCUS       DACBZN010000206         2041 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.214, whole genome shotgun sequence.
ACCESSION   DACBZN010000206 DACBZN010000000
VERSION     DACBZN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.214"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            3..905
                     /locus_tag="IVD64_002909"
     CDS             3..905
                     /locus_tag="IVD64_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6150106.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            937..1179
                     /locus_tag="IVD64_002910"
     CDS             937..1179
                     /locus_tag="IVD64_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6150107.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(1435..1806)
                     /locus_tag="IVD64_002911"
     CDS             complement(1435..1806)
                     /locus_tag="IVD64_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150108.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          785 a          333 c          400 g          523 t
ORIGIN      
        1 ccatgaataa caataacaaa aataataaca ataaaatgaa caaccaaaaa ccagtgtctg
       61 cacatccaga cccaacgaca ccaaaccaat caaatggtgt gaacgatgaa aagaacaaaa
      121 cagcaccagt atctgcacat ccagatccag tagtccctaa cgttcaacaa ggagtagaag
      181 agaagaaaca tgatgcagaa aagcatgcac ctcatcatcc aggaactcca gaatctaaag
      241 ttccagaaca acatgatgca atcgatcctt ctaaacctgt ggaagagcaa aaacatcctt
      301 ctgaccatgc gaaagcacaa gaaaaactta gttctgacca cacaaacgga caaggaaaac
      361 ttggttctga tcatacaaat aaccaaggaa agcatgattc tgaacaggaa gattcaatgc
      421 gcggtgagct gaattatgaa gataaagttg tgcaaaaaat catcggaatc gcaattgaac
      481 aagtagacgg tttattatct gcaaacggtg gtttcttctc taatgtggca ggaaaacttg
      541 tgaatacaga taatgtaacg gctgggatcg aaacagaagt cggtaaaaaa caagtagcag
      601 ttgatatgga tgtcattgtg gaatatggca aagatatcga aaaaatcttt gaagaaatgc
      661 aagaagtaat cggcaaagaa gtaaaaaata tgacacattt agaagtgatc gaagtcaacg
      721 ctaacgtagt ggacatcaaa acaaaagaag aatttgaaaa agatcaagaa acggtgcaag
      781 acaaagtaac tgacgcagcg aaaaaaaccg gtgaatttgc ttcgaatcaa acagataaag
      841 taaaatcagc agctggaagc ggcgcacaaa aagtaaaaga aaatacagaa ccacgggttc
      901 agtaataagg aagtaaaaca ttaggaggaa cgaaacatgg gatttatttg gtcacttatc
      961 gtcggaggaa ttatcggagc aattgcagga gcaatcacga ataaaggttc ttcgatgggg
     1021 attattgcaa atgtcgtagc aggtttagtt ggatcagcac ttggtcaagc attattaggt
     1081 tcttggggac caagtattgc aggaatggca atcgtgccat cattgattgg tgccatcatt
     1141 ttagtactaa tcgtatcatt ctttgctcga aaactgtaaa aagactttta aaaagaggtc
     1201 gtgaaaaagc tgttcttcat caagtaataa gaacagccaa acaaaaatag cttctcatat
     1261 ttttcatttg gttgggcata ttatcgaaga tgcaagcttt tgaacactgt ttatacggaa
     1321 taagaggctg tgacaagact tttgtcacag cctcttattt atgcgggcaa attcccaatt
     1381 agatttttaa aagtttttcc ttaaatgtgt ctttattgat acagagaagc ttctttaata
     1441 aaacaatgcc tccctaaatt ttcttgagat aggaaaggtg aacatacaaa ataatacgac
     1501 cactttgaaa acccagatga tccacaaatg gatcgtatga tactgatgaa gtaaaaagca
     1561 atggatcaga aactcaaaga gattgaaaaa gacaaggaag aaataatatc tcttttttac
     1621 tcttgtctgt tttagccaat aatagctcgt aaagtaatct agacaaacaa atagaagtaa
     1681 acaaagtttc gtaagagcag caatcgtatc atgttgctct gctagacgat tgacgagcag
     1741 gaaaaatgtt ggtaatggaa tatagatcac gagtaatgaa agaaaaaagt aaacaataaa
     1801 tggcatacta ttcctcctaa ttgtgtgagt tcgccctcat tttaaaataa cgatatttgt
     1861 ttgtgaagct ttttcgtgtc gtaacaaatg ttatctaatg tatttttaaa taaataatca
     1921 ttattatcaa aataatgtca gactgtagac aaagtccatt ttatggatga tgtctacagt
     1981 cttttctttt ataatagatt tatgaaaacg aatgcaagga gtgatagcta tgatgtctaa
     2041 a
//
LOCUS       DACBZN010000207         2027 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.215, whole genome shotgun sequence.
ACCESSION   DACBZN010000207 DACBZN010000000
VERSION     DACBZN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.215"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            484..663
                     /locus_tag="IVD64_002912"
     CDS             484..663
                     /locus_tag="IVD64_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150109.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            722..1078
                     /locus_tag="IVD64_002913"
     CDS             722..1078
                     /locus_tag="IVD64_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150110.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          718 a          275 c          386 g          648 t
ORIGIN      
        1 tattacacca tgaaaaagga agacaacgaa aatgaataag gtgatttatc aactaacaac
       61 ggattccata tcaacgattt gaaagaaaaa aaggtcaata atcatttata ccgaaaagtg
      121 agaatgtgtg aaacaaggac aaaaaaagcc tagtaactaa ggaaatggtt cttatttcgc
      181 accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataaacggg caaagcccga
      241 taacttaaga gaaagaaggt gatccatttg aacaagatta ttcccttatt gttagtactg
      301 tttgttggcg aaatacttat gcgagatagt gcagctgtac agttaaacct cgctgtattt
      361 ttggtaatac tggtgttttg tttgatcttt tggcggtttg tattagtcct cgtgagtcta
      421 ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa ggagtgagta
      481 ccaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga aaagagacaa
      541 aaagaattgc agcaactaaa gcagaagtat ggcaaaagtg aatctaagat tatgtgtatt
      601 gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atgtaccagc gctaaaaaag
      661 tgacgtcact ttttaatttg atctatataa gtgatatcac ttttaccaag gagggaatta
      721 aatgttaaaa actaaaaaga attcgtacaa attaaagctt agtatactat ctattatttg
      781 tcttttaggc atgataatta gtggtcattt gtatagagtg gcatatacgc agtttattga
      841 gtacaaggtt cactctttag gattagctgg ggatcgatac gttgaatatg tccattattc
      901 attaaaatgt tcttcatata ttcttctttg gcttattttt tcattgtttg aattgctaaa
      961 tttaatagca atatcttcat atcacttaga taagctaaat accaaatcta gccgttataa
     1021 cgaagtgttt aaggaaaatg tgaagttaga aagtgactta aacaagctaa aaaaataaaa
     1081 agaagaaaac ataagttaac taaaattatt ttattaagaa tgatggaata taaggataag
     1141 gttaccaaca taacgatcat tacttctctg atatgctatg gacttctatc aatcagtatt
     1201 ttgtctatag attatggagt aactatactg caagccttag ttttggctat gcctatgtat
     1261 ctatctttgt ttatcatgca tgaaatatat aagctatttg ttttatcaaa aaatgtagaa
     1321 atacgggtaa atattttggt gaatagttat caaaaagaac tcaaagaaag aagacggtgg
     1381 cggtatttat tgaaaggatg aaaaagtagg atttgacata ctggtattca gaaagtgccg
     1441 tcacttttaa aacaatggaa aaggtggaga taagatgaag aaaaaacaaa ctaaactatc
     1501 attagcagat atttaacaca gttaaccgca actgaagatg gtgttgttga atttgaacaa
     1561 tctgaaattg cggtagtaga tgatcgttac cttaaaatgc cttacttctt tgaccaagca
     1621 aaggtcattt gcctttgtca ctacgatggg gtacgtgatt ttaggcgtta aaaagtagtg
     1681 attatatcaa ctttacgagt aaatataaat ttagacatga aactgaatta tccaatggaa
     1741 aatcatggtt tataatctag aaaaattata gtaatgattg gattatatat attctttcgt
     1801 attttcgtac aactgtagct tgtcaaaatt gagaaattta accgattagg tactttacat
     1861 cgttatttgg gtaatttcta aaacatgaag cttaccaacg aataaactga gactatctta
     1921 ttaattgatc aatataagaa aatatctttg aggagatgat gtactatgtt ttcgtctatc
     1981 cacgattggt taggtggtct tttcggcggt ggaggtaaag gttttta
//
LOCUS       DACBZN010000208         2026 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.216, whole genome shotgun sequence.
ACCESSION   DACBZN010000208 DACBZN010000000
VERSION     DACBZN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.216"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            200..742
                     /locus_tag="IVD64_002914"
     CDS             200..742
                     /locus_tag="IVD64_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150111.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IVD64_002915"
     CDS             898..1347
                     /locus_tag="IVD64_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP6150112.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2026)
                     /locus_tag="IVD64_002916"
     CDS             complement(1377..>2026)
                     /locus_tag="IVD64_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP6150113.1"
                     /translation="IMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEA
                     IVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKL
                     FHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFET
                     LAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          731 a          317 c          386 g          592 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattc
//
LOCUS       DACBZN010000209         2019 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.217, whole genome shotgun sequence.
ACCESSION   DACBZN010000209 DACBZN010000000
VERSION     DACBZN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.217"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            101..571
                     /locus_tag="IVD64_002917"
     CDS             101..571
                     /locus_tag="IVD64_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP6150114.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="IVD64_002918"
     CDS             793..1896
                     /locus_tag="IVD64_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6150115.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          327 c          460 g          545 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcata
//
LOCUS       DACBZN010000210         1999 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.218, whole genome shotgun sequence.
ACCESSION   DACBZN010000210 DACBZN010000000
VERSION     DACBZN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.218"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..374
                     /locus_tag="IVD64_002919"
     CDS             <1..374
                     /locus_tag="IVD64_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP6150116.1"
                     /translation="VSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRRF
                     PEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDDW
                     QKVMAAEVIATYLDGDLVYKK"
     gene            422..1822
                     /gene="yfcC"
                     /locus_tag="IVD64_002920"
     CDS             422..1822
                     /gene="yfcC"
                     /locus_tag="IVD64_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP6150117.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1855..>1999
                     /locus_tag="IVD64_002921"
     CDS             1855..>1999
                     /locus_tag="IVD64_002921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6150118.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          551 a          339 c          432 g          677 t
ORIGIN      
        1 atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt gtaacagtca
       61 ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt ataagagcag
      121 tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc ttcacgacaa
      181 ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta tcggagtacg
      241 gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac ctctgtactg
      301 atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat ggtgacttag
      361 tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga gaggttaaaa
      421 aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga tttttatctt
      481 aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg agacagttgt
      541 tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc accaaatcga
      601 taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg aagggattat
      661 ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg ggattatcct
      721 aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg gagacaaggg
      781 gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa cctttggcat
      841 gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt ctcttggcta
      901 tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat atgttgggtc
      961 gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt acggaaatcc
     1021 cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa ttgttttcac
     1081 catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt atgagagtga
     1141 tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt caacacaaga
     1201 taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg gtatcatttc
     1261 caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc tttttgctgg
     1321 gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag gctgttcaga
     1381 tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa tcttggaagg
     1441 tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg gtcttccaaa
     1501 attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt ttgttccatc
     1561 ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg atctaatgaa
     1621 tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa tgaatattat
     1681 ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta atttctctaa
     1741 atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg ctgttttgtt
     1801 atacattgct acactctttt agtcttaatc atccattaat aggaggcaat agttatgatt
     1861 aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct caaagaattt
     1921 ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg tgctccgcat
     1981 tttttcatag atgctttta
//
LOCUS       DACBZN010000211         1980 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.219, whole genome shotgun sequence.
ACCESSION   DACBZN010000211 DACBZN010000000
VERSION     DACBZN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.219"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(325..529)
                     /locus_tag="IVD64_002922"
                     /pseudo
     CDS             complement(325..529)
                     /locus_tag="IVD64_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(729..1745)
                     /locus_tag="IVD64_002923"
     CDS             complement(729..1745)
                     /locus_tag="IVD64_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6150119.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          558 a          459 c          309 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaaa attttcctaa ttgtgacatg gctgtcacaa
      541 ttccaaatgc tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa ggagccgaaa
      601 gcgtgtcacg ctctcaactc cttttacgct ttattttgct tcatgcaaaa tattcgctta
      661 tcgtgaattt acgtcactaa acattgacgc aaatccactc tcactcatct gacttttctt
      721 attcggtatc acttctctgt cacatccaac cccaaaagcc acgtatccaa ttcttttttt
      781 aattcgtctg cagattcctt aaacgtctct cgtaaatgat tcgttaactc atcattttga
      841 ctttcttgct tcagcaaatt ccgatattta tattttgtat gtctagcaat tatcgaccaa
      901 ttagaaggct cagctatcaa accatcaata actattttat caataggttt tatatcaatc
      961 ggcaaaaaat gcgggcttgc aaaactatct aagctttcct gaaccatcgc atgccaatcc
     1021 gttgctttgc cacgtcttag ctgcatttct aaccgccacc acgtatcgat ttcttttggc
     1081 acgattactt tttttcgttc ctgctctagc tttttgttgt acagtcgcac ttggcgctca
     1141 ctggcacgac tgccccagta cgctgtttct agcttgccag aacggccgta gattggctta
     1201 aaagaaatcg ggtctacaat acgatactga gtaataaagt catccggcac atctacaata
     1261 tcgcaggcaa tatccgcccg ggaaaaatgc ggatctaaaa ataggtcatg aatgaatcct
     1321 ttcatggaac tagctaaaaa ctggcctatc ttattcggat taaaatcaat acgacctttt
     1381 ccttcatgaa atttaagtat ttcgatatac gcaatatctt ctccatattg atcgaataat
     1441 tgccagccgt ttatcccaac tgatttcaaa tatccctgat tttcgttcaa acgcattaat
     1501 tgctcgaaat cgacaaaaac aactccattt ttcgtccaat aagctatggt atcattcagt
     1561 tttccgacaa ttgtaattcg atcaatcgac caatttaatt ttaggggggt ggtcgtacct
     1621 tgcacactta accgcctgtt agaaatacgg ttaagttttt cgagctgatt tatcgttgtt
     1681 cttttttctg cccctcgacc ttcaattttt gattgaattt cactcaactg attttcattt
     1741 gccattttcg tcctccaaac gaaaaaagag acttttggaa agaatttcca gagttcacgt
     1801 aaccctaaaa attcatatcc aaaaatctct agcttgaaga aacctaaaaa tattcgtata
     1861 atgaacctga gttacggagc taggtgaagt agtttccgta aatagtcgag agtgttagcg
     1921 cactgctcgg cttttatttt gctctttttt ctcgttttct ttgattatag cactcctcct
//
LOCUS       DACBZN010000212         1971 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.220, whole genome shotgun sequence.
ACCESSION   DACBZN010000212 DACBZN010000000
VERSION     DACBZN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.220"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(61..240)
                     /gene="rpmF"
                     /locus_tag="IVD64_002924"
     CDS             complement(61..240)
                     /gene="rpmF"
                     /locus_tag="IVD64_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6150120.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(298..867)
                     /locus_tag="IVD64_002925"
     CDS             complement(298..867)
                     /locus_tag="IVD64_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP6150121.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1053..1955
                     /locus_tag="IVD64_002926"
     CDS             1053..1955
                     /locus_tag="IVD64_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6150122.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
BASE COUNT          586 a          419 c          332 g          634 t
ORIGIN      
        1 aaataaacct caaggctcca tggttagcct tgaggtttta cattttctaa ttaaaatgta
       61 ttaagcttct tttgacatta cgtccttacc atcatagtaa ccacaatttg cacatacgtg
      121 atggcttttt ttcatttcac cacagtttgg acattcgttt aaccctttga ttgtcaattt
      181 gtagtgtgta cgacgttttg cttttttagc ttttgatgtt cttctagctg gtactgccat
      241 tttgctacac ctccttgtaa agtcttgtat ctaaattaga cacaatatat tccaatctta
      301 cttgttatcg tcgtcttttg atttagaatc aaaaagttca gatagttttg caagacgagg
      361 atcaatttta ttctcggctt cttccatttt tctgcgctgg tattcttctt cggaaacaac
      421 ttcccaatca ttgccttttg gaagttctgt tgctgcttcc tcttcttttg tcaatacccg
      481 cattggaatc gccaataaga tgttgtcttc aatcgactct gctaaattca ataatggacc
      541 ttcaattagc aaaatctcat ctgcagacat ttgttcatct ctactttgga attgttcttc
      601 tgtcatgaat acttcgtcta cagaaaaatc catgggcagt tcaacaggtt ctagtgaacg
      661 tgttgaagga actgtcaaag tcgtctgcac agtgtaatga acgatatagc ctttcttatc
      721 aactgagacc aaaccttcga cttccactgg cgcaacatca agtattaaat tgtctcgctt
      781 catgagagcg gctttcaagt cgagcgtctc atgaaaagtt aacggtgtct cctggtattt
      841 tctcaattcc aataaagacc atttcatttt tcatcactcc taagcaacaa aagcaattat
      901 acagggcaaa aagcatgttg tcaatgaaat tttcttgaca ccttgcagtt cataaaaaaa
      961 cagaaaaatc tttattagaa agcctctgtt tgctatcatc ctcagaatca tttacaataa
     1021 tcattattcc attaaaggag cggatgatct tcatgaacac gaaagatatc gaatactatg
     1081 tccaaatcgt caaacaaaaa aattttacga aagtggctaa ttatttccgt gtgagccaac
     1141 ctactattac atatgcatta caacgtcttg aaaaagagtt tgacaccgta cttattacaa
     1201 gagatcgatc gcatcacgaa ttaatcgtca cgccttctgg aaagcagctg tttctccatg
     1261 cacaaaccat tctccaagag atttctactg caagagctga aatcactcag ttgcaaaaga
     1321 aaaagcttcg ttttggcatg cctcctatta tcggcaatga atactttcct aaactgtcaa
     1381 gttactttat caagcatgaa ttgatgaatc aaattgagat taccgatgga ggctctcggg
     1441 acttatacgg gatgatccga caagggagga tcgatttagc tgtattaggc tcaacacagc
     1501 caatcgtgga tcaatattta gaaacagagt tacttttgga caaacgtttc atgatcgtcg
     1561 tgccacctac acatcctctt tccaagcagg atactgtctc tttcaaagat atagcagatg
     1621 aacagtttgt actattgaac gaacattatg tccaccccgc agcattcact aagctttcta
     1681 aacaaacaca tactcatcca aaagttgttt atcaaagcaa tgacttgacg attttaaaga
     1741 gtatggtccg agaaaatgtg ggtatcggtt ttttggcaga gattgctgtc catcctgaag
     1801 atcatttagt ttgcctccct ctttcagatg aagttcagcc tcgcttcttg atttcattgg
     1861 caaaacgaac acagcagatc gtcacgcctc ttcaagaaca agtagtccaa gttatccatg
     1921 aattcgttac agcacaaaaa aacagctctt cgtaaaagaa gagctgtttt t
//
LOCUS       DACBZN010000213         1896 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.221, whole genome shotgun sequence.
ACCESSION   DACBZN010000213 DACBZN010000000
VERSION     DACBZN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.221"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(366..1484)
                     /locus_tag="IVD64_002927"
     CDS             complement(366..1484)
                     /locus_tag="IVD64_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150123.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT          556 a          407 c          297 g          636 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt caacacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctc
//
LOCUS       DACBZN010000214         1843 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.222, whole genome shotgun sequence.
ACCESSION   DACBZN010000214 DACBZN010000000
VERSION     DACBZN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.222"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..219
                     /locus_tag="IVD64_002928"
     CDS             <1..219
                     /locus_tag="IVD64_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150124.1"
                     /translation="GEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSIGFESIPRT
                     RFVRIFSRFQRVSISSFRPAVKPKPFDR"
     gene            complement(284..1425)
                     /locus_tag="IVD64_002929"
     CDS             complement(join(284..1197,1197..1425))
                     /locus_tag="IVD64_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6150125.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          503 a          348 c          389 g          603 t
ORIGIN      
        1 ggtgaaaaat attttatcat tcaagaatat gagggcaagt tagcacctta ttatactact
       61 tgggaaatga atgaagaggg aatcaataac tttccatgta aaagcattgg atttgagtca
      121 atacctcgga cacgatttgt gagaattttt tcgagattcc agcgggtgag tatctcatcg
      181 tttcgaccag cggtcaaacc aaaaccgttt gaccgctgat cgaagcgatg aagttgaaac
      241 cctgtggaaa tctcgccaaa aaaatgtaag tggtagctag gaactaagct tgcaatgcca
      301 actcctccat ctgatttggt gttaaatagt tgatactact atggattcgc ttgcgattgt
      361 aaaatccttc aatgtattga aataacgcta aataagcagc ttcaaagttt tcatacgctt
      421 ttgatggata gacttcttct tttttgagtg aggcgtgaaa cgattcgata ccggcattat
      481 cgtagggaca gcccttacga ctgaaggagt gccgaatgtt caattcattc agctttgcct
      541 cataggcttc gctagtgtac tgagaaccca aatctgagtg gatgatcaag ttctcggagg
      601 gtgtttggtt taagagcgca ttgtcaaggg tttcgataac caattctttc gtcatctgct
      661 tgccaaactt ccaaccgatg actttctttg tgtggaggtc ttgaatagtg gagaggtagg
      721 tccagccatc ttttagggta tgaatatagg taatatctgt cacccatttt tgattgatcg
      781 acgtagtggt aaaatcttgg tttaacaggt ttttacgtcc ttcactttta actttcgctt
      841 gtttgccagg cttgtacttc ttgagggtga ctgaacgcaa gcctaatttt ctcatcagta
      901 tttgtacttt ctttaaggac aaggtctcgc cacggccaac aagaatctta tgaattttag
      961 gcgcaccata gatacccttg ttttcaaagt agattcctga aatcaagcgg tttaagcgtt
     1021 tgttttctga ttggcgcttg gaaggtttct ttttcttcca gtcatagtag ctggatttcg
     1081 gtgcttccaa aacggtacac atttgtttta cgtcatgatc atgaacattt gattgaataa
     1141 attcgaagac atcactcatt tttacttttg agcgaatatg gttaaagcct tttttaggat
     1201 agtgttttcc tcctgcatgc gggccatttc ctttttcatc gctaggattt ctgcctcagt
     1261 catgccggtt gctacattct ttgtatgaag cttcttccaa cgataaatcg tttgtgtagc
     1321 gataccatat tgcgctgcca gttcttcgac cgattggcca gactggttta aatctaagat
     1381 catcttctta aagtcttcat caaatttttt cttggctttt gccatcgtgg acacgtcctt
     1441 ttcttagtag ttagattcta ccaaatcgtg tccatgaaat catcctaaca tcacattgaa
     1501 gagtccattt cactcacaga gtgttagttg ttaagtgaat aaaagaagta aaagagtcct
     1561 gaacgtgatt gtccaggact ctttttttca atgaattgtt ttccgccaac tcattgagtg
     1621 gtgcgatttg cgactacatt atatcaaaag aaagagaagg gaggaagcag aaatggttca
     1681 ttcaacgatt ggagttgttg ttgaaaaaag cagagacaat ctcgtttttg tgacagaaat
     1741 tcaaacaggc agatcttttg tcgtcacaga caaagcggca aaagcttatc aaagtagcga
     1801 tattttaacg ttaaatctga ctacgaagac gttcgtcgat gcg
//
LOCUS       DACBZN010000215         1737 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.223, whole genome shotgun sequence.
ACCESSION   DACBZN010000215 DACBZN010000000
VERSION     DACBZN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.223"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            174..596
                     /locus_tag="IVD64_002930"
     CDS             174..596
                     /locus_tag="IVD64_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150126.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            606..809
                     /locus_tag="IVD64_002931"
     CDS             606..809
                     /locus_tag="IVD64_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150127.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1020..1604
                     /locus_tag="IVD64_002932"
     CDS             1020..1604
                     /locus_tag="IVD64_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6150128.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          615 a          238 c          309 g          575 t
ORIGIN      
        1 tcacttttct ttttaaatgg caatattttt tgtaaataaa tgtcaatcaa gaaaatttaa
       61 acgattatga cattaaaaat atattttatg aattttctag tatataactt gatttatctg
      121 ttaagattta ttaagaaaac gttttcttag ttttgatttc gaggtgatag acaatgaaaa
      181 ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa
      241 ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat
      301 taactccttt agatagggac ggaatagcca ttggtaaccc aactaacgac ttaactgtta
      361 ctgaaaatta tttaatagtt tctccattac ttgctctagc agcttctaaa acgatacgag
      421 tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga
      481 attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata
      541 aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat
      601 tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta
      661 aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac
      721 aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag
      781 accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat
      841 tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt
      901 gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca atatctatgt
      961 tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga gtgatttttt
     1021 tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta caagaagaca
     1081 gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg gcaaaaagta
     1141 atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca cttgtcgttt
     1201 ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc aatcgactga
     1261 atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac aaatcaagtt
     1321 ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag cgtaatttga
     1381 tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc
     1441 gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg gatagcggta
     1501 cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt tatcgttatt
     1561 tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg tcatttaatg
     1621 ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt tttgtaagtg
     1681 tctactgata acttattctc aaatgttgta aggattttat tcatttgtta atattaa
//
LOCUS       DACBZN010000216         1732 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.224, whole genome shotgun sequence.
ACCESSION   DACBZN010000216 DACBZN010000000
VERSION     DACBZN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.224"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            361..1611
                     /locus_tag="IVD64_002933"
     CDS             361..1611
                     /locus_tag="IVD64_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6150129.1"
                     /translation="MKLFIGLDVSSQKLDTCFLTDSKEILFEGSLSNDLIGASEIKQR
                     ILEYQETFGFYQIVIGMESTSVYSFHPAMFFYLDEDLKLLPVEVTVENPYRIKQYSRM
                     FDQDKTDKIDAQIIADYLRVDLHTLSPIKEEVYVGLQRLTRSRYQLVTQMVEAKQHFL
                     ENLTYKCNTLSKELANEDNKTSIFGASIMNLLTEGITLDEFSEMPLEAAVELLQKLGK
                     GRFKNPEQLVKSIQKAVRSSYRLGKVTKNSIDLVLGIIANEIKFLKKQIKEIEKAITA
                     LLETIPEAQCLLSVPGLGAVYTAGLIAEIGQIERFDDQSKLAKYAGLTWTKNQSGNSQ
                     SENTPLNRSGNRYFRYYLIEATNRVRSTLPEFKEYYQKKRNEVPKHKHKRALVLTGRK
                     FVRLIDALLRNGQFYQPAKVVIEK"
BASE COUNT          601 a          311 c          303 g          517 t
ORIGIN      
        1 acgacttcca tcgtttcagt caccccttag ggctattcct tattaaataa cttttaaacc
       61 ttggctattg aattttgttc ctggataata gacttatgga aacggtctat cggaacactg
      121 ctaatttcct ttgtagctat gactacagca ggaagcctgc atcccgactt attgattatc
      181 atgcctcaaa ctcattagct gtaccgttca cttcttaaac tatttctagt aggaaatagg
      241 gtggctttta agaagtaaac aattgagtac tattaagcga gactgcggtt attcaataag
      301 attcagggat aaacttcaat agctaagtat tccaaaaaat ttatgaaagg aggaccttcc
      361 atgaaattat ttattggttt agacgttagt tctcaaaaat tagatacttg ttttttaaca
      421 gactccaaag aaatcttgtt tgaaggttca cttagcaacg atttaatcgg agccagcgaa
      481 atcaaacaaa gaattctcga ataccaagaa acctttggtt tctaccaaat tgtcataggt
      541 atggaatcaa cttctgttta tagtttccac ccagcaatgt ttttctactt agatgaagat
      601 ttgaagctat tacctgtcga agttactgta gaaaatcctt atagaatcaa acagtattca
      661 agaatgttcg atcaggataa aacagataaa attgatgcac agatcatagc tgattatctg
      721 cgagtagatc tccatacatt gtctcctatt aaagaagaag tttatgttgg acttcaacga
      781 ctaacaagat ctcgttatca attagtgaca caaatggtag aagcaaaaca acatttttta
      841 gaaaatttga cgtataaatg taatacttta tcaaaagaac ttgcgaatga agataataaa
      901 acttccatat tcggagcttc gattatgaat ctacttacag aaggcattac cttagatgaa
      961 ttttcagaaa tgcctctgga agcggccgtt gaattacttc aaaagctagg aaaaggtcga
     1021 ttcaaaaatc cagagcagct tgtcaaaagt attcaaaaag ctgtacgtag ttcttatcgt
     1081 ttaggtaaag taaccaagaa ttccattgac cttgtattag gtataatcgc caatgaaatc
     1141 aagtttctaa aaaaacagat taaagaaatt gaaaaagcca ttactgcttt acttgaaaca
     1201 attccagaag cccaatgctt attaagtgtc ccaggattag gagctgttta tactgcgggc
     1261 ttaatcgctg aaattggtca aatcgagcga tttgatgatc aatccaaatt agcgaagtat
     1321 gctggtctaa cttggactaa aaatcagtcg ggaaactctc aatctgaaaa cacgccacta
     1381 aatcgttctg gaaatcgcta ttttcgttac tacttaattg aagccaccaa tcgagtaaga
     1441 agcacacttc cagaatttaa agagtactac caaaaaaaga gaaatgaagt acctaaacac
     1501 aaacataaac gagcgctcgt cttaaccgga agaaaatttg tacgcttgat cgatgcgcta
     1561 ctacgcaacg gtcaattcta tcagccagcg aaggtggtga tagaaaaata acgtaaagtt
     1621 atttgcgaaa atcttcgtga tcaacccgaa aaaaatttga ttttggtcgg gtctagttca
     1681 gcgcggtctg atttttcaaa atctaactca tattctaaaa tttatatctt ga
//
LOCUS       DACBZN010000217         1730 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.225, whole genome shotgun sequence.
ACCESSION   DACBZN010000217 DACBZN010000000
VERSION     DACBZN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.225"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IVD64_002934"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IVD64_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6150130.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /locus_tag="IVD64_002935"
     CDS             complement(1305..1709)
                     /locus_tag="IVD64_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP6150131.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          509 a          300 c          274 g          647 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg
//
LOCUS       DACBZN010000218         1718 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.226, whole genome shotgun sequence.
ACCESSION   DACBZN010000218 DACBZN010000000
VERSION     DACBZN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.226"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(606..962)
                     /locus_tag="IVD64_002936"
     CDS             complement(606..962)
                     /locus_tag="IVD64_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP6150132.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IVD64_002937"
     CDS             complement(976..1386)
                     /locus_tag="IVD64_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6150133.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          529 a          315 c          242 g          632 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aaggactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcac
//
LOCUS       DACBZN010000219         1684 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.227, whole genome shotgun sequence.
ACCESSION   DACBZN010000219 DACBZN010000000
VERSION     DACBZN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.227"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(45..779)
                     /locus_tag="IVD64_002938"
     CDS             complement(45..779)
                     /locus_tag="IVD64_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6150134.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IVD64_002939"
     CDS             1105..1515
                     /locus_tag="IVD64_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP6150135.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1508..>1684
                     /locus_tag="IVD64_002940"
     CDS             1508..>1684
                     /locus_tag="IVD64_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP6150136.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYN"
BASE COUNT          535 a          294 c          325 g          530 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga aatggaggag
     1501 aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga ctttaggagt
     1561 gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taat
//
LOCUS       DACBZN010000220         1636 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.228, whole genome shotgun sequence.
ACCESSION   DACBZN010000220 DACBZN010000000
VERSION     DACBZN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.228"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(183..1502)
                     /locus_tag="IVD64_002941"
     CDS             complement(183..1502)
                     /locus_tag="IVD64_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP6150137.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DACBZN010000221         1610 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.229, whole genome shotgun sequence.
ACCESSION   DACBZN010000221 DACBZN010000000
VERSION     DACBZN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.229"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            262..1194
                     /locus_tag="IVD64_002942"
     CDS             262..1194
                     /locus_tag="IVD64_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6150138.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1266..>1610
                     /locus_tag="IVD64_002943"
     CDS             1266..>1610
                     /locus_tag="IVD64_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150139.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          681 a          192 c          255 g          482 t
ORIGIN      
        1 attttgaaat aagactctat tattacaagt tcatccgttg taagatgggt ataggtcatt
       61 tgtactcact ccttataatt ctttggtcgg aactattttg agtgtagcac aaatgacttt
      121 tttagttgtc tagcttaatt ttacaatcgg cgtgatgaaa taaatcaaaa gtatgatgaa
      181 acattaaaaa atgtctattc ttcacttatc gtttcaaata tagaagaaac ttcatcttct
      241 gaaataaaaa ataagctttc tttgttaaaa ttaaaagaaa aggaatatat tattgacgat
      301 gctgtttctg tccctcttat tttaaataaa cgagagaaaa ataatttaaa gaaaaaaatg
      361 ctcattccat ttgattcaga agtgaaagga ttactcaata aactaactat ttctaatata
      421 gactcgacta gggaaaaaat cataaaaata tttgataaaa ttaataattt attcaagaat
      481 gacaatgctg aattaaggaa gggtctttta gagtttaaac ttcaagaact agaacttcat
      541 tactcttatt tagtgaaaat taccgaagaa aaagaacaac aaaaagcaat aaaagaacag
      601 atgatagaag aggaaaaagt tcgtagagaa attgatcgtg aaaagaaacg tattgataaa
      661 gaacaacgtc aattcaattc tgaaatttcc aaattaatcg catatatgca aaaagctaat
      721 gcagatgtgg aaaaagaatt atatgcaaat aaaattcaag aactggaaga aaaattaaaa
      781 gaattagaag ttatacgtga aaatgtatta caaagagaat taaatactag agccggttat
      841 gtgtatgtaa ttagtaatat tgggtctttt ggtgaagaca tatataaaat tggtatgact
      901 agaagattag aacctatgga tagaataaaa gaattgagta gtgcatcggt tccatttgaa
      961 tttgacgttc atgcgatgat tttttcagag gacgctccgt ctttggaaac taaattgcat
     1021 aatcatttta gaaaacaaga agttaataaa attaatcaaa gaaaagaatt tttcaaagtt
     1081 tcactagacg aaatagagaa agttgtttta gaaaattata atggaacagt aacatttaca
     1141 aaattagcta aagctgaaca atatagacga tctttagaat tgtcaaaaga ttaaatatat
     1201 aaaaagcctt cgggcttttt cttttaacaa ttaataggaa cgtatattcg aaaggaatag
     1261 aaataatgtg gttagaaaaa ataaatgaca aaaaatttaa gtacaccgaa cgttatacgg
     1321 atcccttaac tgaaaaaaag agaaaagtgt ccgttacttt atctagcaat tcacgccaag
     1381 cttggaatca agctaactta cttttgaatg aaaaaatcgc agaaaaaata aaacgaaatg
     1441 aagaattacc actaactttt ggagaattaa aaactaaatg ggatgaaaaa tacaagccaa
     1501 ctgtaaaaga aagttcctat cgaacaactc aggtttatct atcattaatt tcaaaatata
     1561 taaaagatga tgtacttgta aaaaacgtta atagtaatct tatacaagat
//
LOCUS       DACBZN010000222         1608 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.230, whole genome shotgun sequence.
ACCESSION   DACBZN010000222 DACBZN010000000
VERSION     DACBZN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.230"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            206..469
                     /locus_tag="IVD64_002944"
     CDS             206..469
                     /locus_tag="IVD64_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HAP6150140.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            469..804
                     /locus_tag="IVD64_002945"
     CDS             469..804
                     /locus_tag="IVD64_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6150141.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            878..>1608
                     /locus_tag="IVD64_002946"
     CDS             878..>1608
                     /locus_tag="IVD64_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6150142.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGT"
BASE COUNT          545 a          229 c          349 g          485 t
ORIGIN      
        1 ggtaaatcaa tgacgaactc agaactagaa tccattcctc aacaaaatca aggagacgct
       61 gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa
      121 ttgaaattgt ttgatggtgg aaagtaactt cttgtatttt ggtgtataca agtatatact
      181 aatgtgtgag agaaggaggg attccatgtt agtaaaagaa atcgaaatga aaaaatgggg
      241 aaattcacaa ggtattcgtt ttggaaaaga agaattgtca gttctaggag aaactaatac
      301 tgaaaattta aaattcgaaa tggtggtaac agaaggaaaa attgttttaa ctcctaaaaa
      361 taaaaagcca gaaactttag atgaattgtt cgccaattat gaaggagaac ccttaggaaa
      421 agaagacaaa tatgaatggg gaaatatgca aggtagggag atcatttaat gaaaaagaac
      481 tatcatgatt tatcgcaagg agatcttgtt tatattgatt taaatcctac caaaggacat
      541 gaacaacggg gaaaacgacc atgtattatt ttatctaaac ctaataaata tttgaactat
      601 atgattggtg tcgcaccgat aacaacaaaa gcgaagcctt ttcctgtaca tgttgatttg
      661 aatggtacgc aaaaaataaa aggacaaatt cttttggaac accatcgaat gattgacatt
      721 gagagtagag gttttcagtt tgtggaagct gtgccaaagg aaattttaga agaatgtatt
      781 gaaaaaatta aattatttta ttaaaagcag cgaagaataa ttattatttc ttcgcttttt
      841 tgttgtttaa aattaaaaag ttaggagtac tgactatatg gaaaatgata caaatatagt
      901 tatggaacat ttgaagcgtc gtgcaaagcg acgcttattt ttgtggttgt ttggtacttc
      961 tgcaggtttg attactattt tgatcactgt gttcgtaacc ctttttctaa tacttgcggc
     1021 gggttcgatt gataattcgg attccgattc aagtagtggt ggcgaagctt ttacaggaga
     1081 gtattcggaa ggcttaccga tttataaaga aatcaaagga agaggccctt tttctgatga
     1141 gattgcgcaa tatgccgtag gtgcagcagt gaaatataaa ttattaccaa gtgtgattct
     1201 ttcgcaatac ggttatgaat cagcgtttgg tacgtcggcg tccgcaagaa atgatctcaa
     1261 ctattttggg attacttggt ttgatggttg tctgtttcct aaaggaactg cacgaggaat
     1321 tggtggcatt gaagggggct ggtacatgaa gtttcccaac tcaaaagctg cattctccta
     1381 ttacggcttt atggtagcca cacaatcaaa ttttaatgca tgtgtaggaa ataaaagccc
     1441 tggcgcaagc ttattgatct taggacgagg tggttatgcc gctgcaggaa ttactgaaga
     1501 tagtccgtat tatacaggtt gtatgtcaat tatcacttct aataaattaa ccgaatatga
     1561 tgaattcgcc atcaaacatt ggggtgaagg tggcaataac aatggcac
//
LOCUS       DACBZN010000223         1584 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.231, whole genome shotgun sequence.
ACCESSION   DACBZN010000223 DACBZN010000000
VERSION     DACBZN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.231"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            292..1479
                     /locus_tag="IVD64_002947"
     CDS             292..1479
                     /locus_tag="IVD64_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6150143.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          316 g          505 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta cttg
//
LOCUS       DACBZN010000224         1559 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.232, whole genome shotgun sequence.
ACCESSION   DACBZN010000224 DACBZN010000000
VERSION     DACBZN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.232"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            32..739
                     /locus_tag="IVD64_002948"
     CDS             32..739
                     /locus_tag="IVD64_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150144.1"
                     /translation="MNNVVYQLTMEDISRFLTNKEHLNEVIRRIQKDFSCMEEMKYFV
                     QNVEEQLYLESETSQTKVTVSNNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKS
                     ELVENFISYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGIDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKQMPYQRFERKKGQ"
     gene            1120..1299
                     /locus_tag="IVD64_002949"
     CDS             1120..1299
                     /locus_tag="IVD64_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150145.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFNIPALKK"
BASE COUNT          605 a          231 c          305 g          418 t
ORIGIN      
        1 tattacacca tgaaaaagga ggacaacgaa aatgaataat gtagtttatc aactaacaat
       61 ggaagacatt tctcgttttt taacaaacaa agaacatttg aatgaagtaa tcagacggat
      121 tcaaaaagac tttagttgta tggaagaaat gaagtacttt gttcaaaacg ttgaagaaca
      181 gttatatttg gaaagcgaaa cgagtcaaac aaaggtcaca gtcagcaata atcgtttaga
      241 tgtgtataaa cgtcgtcctg aatcattaaa aaaaggagcg gagcttctac gactagaaac
      301 agaaaaatac gtcatgaatg tttacctaga aaaaagtgag ttggtcgaaa attttattag
      361 ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa tttttgagag
      421 catggaatta atggaagaag aaatggaaga gttaaacagt aaagatgcga cggtagacga
      481 ttattacctt gtatttacta gatttttaac gtgtggaacg gaatctatcg tgctaatggg
      541 aatagattac gaactcgctg tacccgaagc gtttttgagg gaatatgtag aagaagtaga
      601 taacccaggg gatgattggt acacgacttt aactgatttt ttagacacct acacttacga
      661 cgatacagaa gaaatccaaa atgagcttga ccgtaaacaa atgccatatc aacgatttga
      721 aaggaaaaaa ggtcaataat catttatacc gaaaagtgag aatgtgtgaa ataagaacaa
      781 aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa ccgcccccgc
      841 cgcttaggtg gtgcgaaata agcgagcaaa gcccgctaac caaagagaaa gaaggagatt
      901 tatatgaaaa agattattcc cttattatta gtactatttg ttggcgaaat gcttatgcgt
      961 catgctgcaa ctgtgcattt aaacttcgta gtatttttgg taatactggt gttttgtttg
     1021 accttttggc ggtttgtatt agccctcgtg agtttactat tcaagatgat tgcagccata
     1081 tttttcatag gattatttcg atgagaggag agagtatcaa tgccaattgc aataggaaat
     1141 aaacggttac ccgttacgtt ggatgaaaag agacaaaaag aattacagca acttaaacag
     1201 aaatacggta aaagtgaatc caggattatg tgcattgcgt tagatttatt gattgcgcag
     1261 gaaaaagcag gattcaacat accagcgctc aaaaagtgac gtcacttttt gacttaaaaa
     1321 ataaaagtga tagcactttt atatagtaaa aagaagaata aacgttaagg gatagaaaaa
     1381 gagaaaagaa caaaaaaaga gctaacagta ccattgttag ctcttatgca gtaatgaaaa
     1441 ccaagccttt aaaaaaggag atgtttcatc acaaacacaa acagaaaaat aaaaaaatca
     1501 aaaatctaat aatcataaat tgctaccgaa gcacctttta ttataattaa tcaaatata
//
LOCUS       DACBZN010000225         1502 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.233, whole genome shotgun sequence.
ACCESSION   DACBZN010000225 DACBZN010000000
VERSION     DACBZN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.233"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(312..611)
                     /locus_tag="IVD64_002950"
     CDS             complement(312..611)
                     /locus_tag="IVD64_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150146.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(617..847)
                     /locus_tag="IVD64_002951"
     CDS             complement(617..847)
                     /locus_tag="IVD64_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150147.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1097..1297)
                     /locus_tag="IVD64_002952"
     CDS             complement(1097..1297)
                     /locus_tag="IVD64_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6150148.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          533 a          243 c          219 g          507 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag
      481 tagagattaa ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc
      541 ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca
      601 ttaaaaagca taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat
      661 atcgagtttt tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt
      721 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
      781 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      841 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      901 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      961 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
     1021 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
     1081 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
     1141 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
     1201 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
     1261 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
     1321 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
     1381 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1441 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1501 aa
//
LOCUS       DACBZN010000226         1483 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.234, whole genome shotgun sequence.
ACCESSION   DACBZN010000226 DACBZN010000000
VERSION     DACBZN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.234"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..485
                     /locus_tag="IVD64_002953"
     CDS             <1..485
                     /locus_tag="IVD64_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6150149.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(692..1093)
                     /locus_tag="IVD64_002954"
     CDS             complement(692..1093)
                     /locus_tag="IVD64_002954"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150150.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(1214..1297)
                     /locus_tag="IVD64_002955"
                     /pseudo
     CDS             complement(1214..1297)
                     /locus_tag="IVD64_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1320..>1483
                     /locus_tag="IVD64_002956"
     CDS             1320..>1483
                     /locus_tag="IVD64_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6150151.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTG"
BASE COUNT          482 a          277 c          287 g          437 t
ORIGIN      
        1 taaaggaagc gatcgatcaa atcaagacgg ctgtcttgta tcctcataac ggattaccga
       61 ttttattgat gggtgtaagc ggttcaggaa aaaccttttt ggcagaaaaa atttatgaat
      121 acgccaagga acaacaggtg atagaatcaa atgctccatt tatcgcctat aattgtgctc
      181 aatactttaa tttctcgatt aacaagatcg tagaaaattt aacgatcctt gatgcaatga
      241 aggttttgag attggtacaa aaggccgtca aagaactcga aaaactaatg gggatcgaat
      301 tttcaaacaa tcaattgttc ttgctttaca tgcactgctg ctcaatgatc gaacgtattt
      361 tacgcaaaga aagtgtagat gaacaagcag atatcaaaga atatattcaa aaagagggac
      421 ataattatga aatatcaaaa tacccggctt acaaaacgaa gtttgaaagt actgtaataa
      481 agtaagattt aaataagcat aaacaaggta agaaccatag gggctgtgtc aagtccaaaa
      541 tcttgtgtaa aaaggaaatc tcaaagtacg atgttttgcc ttgttgagcc tagctacggt
      601 ccaatatgct ggtaagaagt gtcaagagca aaaagcgaag cgtctcttga tacttctcac
      661 cagtatatta tactattggc ggcttaatgt gctaaatctc gttacgatca aattcaagat
      721 attttctagg tgcagaagtc cattcgtcat ccttttccat caataaagca ccgatgatac
      781 gaataacaga atcacaattt gggaaaattc ggacaacacg ttcacgacgg cgtacttctt
      841 catttagtct ttcaagaaca ttcgttgttc tcaatcgttg acggatattt tctggtaaaa
      901 ccattacttg catggcatct tcgaagccct catccaatat tttacaggca gttgcgactt
      961 tcttgtcttc cccatattta tccataataa gttgtttacg ttctccagcc agctcgatgc
     1021 ttgccgtttt gaagatggct ttaaagtctt ctttgatatc gcttgagatt ttcttggaga
     1081 atttgtcgga aatgttgcga aggagatgtg cttggcatct ctgctagctc gcacaaagaa
     1141 ggtattcttt cacagctttt gtgagcccta tatgcgcatc cgagatgacc atctttactc
     1201 ctgaaagacc gcgttctttc aatccgagag tgtaaaatat tttgtgtaaa tagaaaaaag
     1261 gaagtccctt cagtagaata gagttaccac aacacattca cagaaaagag gacttccata
     1321 tgaacgattt tactacagaa attctaaaga ctctagcgaa taaaggcgat ttgaatgaat
     1381 tattccgtgt ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt
     1441 tcctcgatta cgaaaagtac gatcgcattg gttttaacac ggg
//
LOCUS       DACBZN010000227         1468 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.235, whole genome shotgun sequence.
ACCESSION   DACBZN010000227 DACBZN010000000
VERSION     DACBZN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.235"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            225..842
                     /locus_tag="IVD64_002957"
     CDS             225..842
                     /locus_tag="IVD64_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6150152.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            883..1182
                     /locus_tag="IVD64_002958"
     CDS             883..1182
                     /locus_tag="IVD64_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150153.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1216..1383
                     /locus_tag="IVD64_002959"
     CDS             1216..1383
                     /locus_tag="IVD64_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6150154.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
BASE COUNT          500 a          223 c          304 g          441 t
ORIGIN      
        1 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
       61 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
      121 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
      181 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
      241 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
      301 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
      361 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
      421 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
      481 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
      541 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
      601 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
      661 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
      721 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
      781 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
      841 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
      901 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
      961 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     1021 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     1081 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     1141 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     1201 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     1261 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     1321 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     1381 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     1441 gctttcttta ttttataaat agttggtc
//
LOCUS       DACBZN010000228         1451 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.237, whole genome shotgun sequence.
ACCESSION   DACBZN010000228 DACBZN010000000
VERSION     DACBZN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.237"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            105..1406
                     /locus_tag="IVD64_002960"
     CDS             105..1406
                     /locus_tag="IVD64_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6150155.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DACBZN010000229         1379 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.238, whole genome shotgun sequence.
ACCESSION   DACBZN010000229 DACBZN010000000
VERSION     DACBZN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.238"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            50..1357
                     /locus_tag="IVD64_002961"
     CDS             50..1357
                     /locus_tag="IVD64_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP6150156.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT          477 a          269 c          294 g          339 t
ORIGIN      
        1 ataaaatttt aatttgtagc gaaacaaatt aattacaagg agatcggata tgtccataaa
       61 aaaaacacgt ctagtcctat tattggctag tctcattctt tgccttgctt cttttctacc
      121 agctgttgct gtgcaagctg aagatacgtt caaagtaaat gcaaaagcag cttttgctgt
      181 agatgcgcaa agcggtaaga ttctttatga tcaagatgga gaaaaaacca tggggatcgc
      241 ttcaatcaca aaaatcatcg gtctttacat cgtccttgat caagtaaaag aaggaaaact
      301 gtcatggggt gataaagttt cgatttcaga ttatgcagaa aatttgagta tcaccccaga
      361 tttatcgaat gttcctttgc ataaagaaaa cacctacact gtcaaagaat tattcgactc
      421 tgcaatcatc cagtcagcca atgcttcaat ggtggccttg gcagaaaaaa tctctggcag
      481 cgaagctaaa ttcactgaac gaatgaaaga acaattgaaa gattggggaa tcaaagatgc
      541 aacaatcgtt aatgcatcag gattgaacaa ctcttatctt ggcgaaaacc gaccagaagg
      601 cactggagaa aacgacgaaa atcaaatgtc tgctcaagat gtggccatcg ttgcccgcca
      661 tttgatcttg gatttcccag aaatactgga tgtatcaagt actacgaccc aaatgtttgg
      721 cgaaaatacg caatcaccag tggaaatggt caactggaac tggatgctcc caggattcgt
      781 taattacaag gaaggcgtgg acggattaaa aactggtacg actgagcttg caggtgcttg
      841 tttcgttggt acgatcacta aaaacgatca acggatcatc actgtcgtat tgaatgcgga
      901 cggccatgcg gaaaacccga gtgcccgctt caatgaaaca gaccgtttga tggactactg
      961 ctatgacaat tggtcagaaa aagagctcgg aaaagctaac gcaagtattc ctagtctaaa
     1021 aaccattgat gtaaaagacg gaaaagaatc aacagttcct gttgtgctta aaagccctgt
     1081 aaaagtatgg gtacgaaacg atatggatac tggcaaatta acgatcacgc caacacttga
     1141 caagaagcaa gtcactgaca atgagatcca agcacctatg gataaaggta caaaaatcgg
     1201 taccgctacg atcaccttgg cagacgataa attaggctat ttagaagaca acaatgcacc
     1261 atcaacagag atcattacgg ataaaacagt ggaaaaagcg aatatattcg tgattggctg
     1321 gcgacgcgtc gcagaatttt tcggcaactt gttttaattg aacaataaga aaaacaggc
//
LOCUS       DACBZN010000230         1374 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.239, whole genome shotgun sequence.
ACCESSION   DACBZN010000230 DACBZN010000000
VERSION     DACBZN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.239"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            33..1372
                     /locus_tag="IVD64_002962"
     CDS             join(33..474,474..1372)
                     /locus_tag="IVD64_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP6150157.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DACBZN010000231         1363 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.240, whole genome shotgun sequence.
ACCESSION   DACBZN010000231 DACBZN010000000
VERSION     DACBZN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.240"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            38..1330
                     /locus_tag="IVD64_002963"
     CDS             38..1330
                     /locus_tag="IVD64_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6150158.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          440 a          279 c          252 g          392 t
ORIGIN      
        1 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
       61 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      121 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      181 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      241 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      301 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      361 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      421 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      481 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      541 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      601 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      661 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      721 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      781 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      841 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      901 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
      961 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1021 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1081 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1141 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1201 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1261 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1321 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DACBZN010000232         1358 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.241, whole genome shotgun sequence.
ACCESSION   DACBZN010000232 DACBZN010000000
VERSION     DACBZN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.241"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            82..>1358
                     /locus_tag="IVD64_002964"
     CDS             82..>1358
                     /locus_tag="IVD64_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP6150159.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          460 a          255 c          259 g          384 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatca
//
LOCUS       DACBZN010000233         1300 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.242, whole genome shotgun sequence.
ACCESSION   DACBZN010000233 DACBZN010000000
VERSION     DACBZN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.242"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            854..1066
                     /locus_tag="IVD64_002965"
     CDS             854..1066
                     /locus_tag="IVD64_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150160.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          482 a          195 c          218 g          405 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg
//
LOCUS       DACBZN010000234         1297 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.243, whole genome shotgun sequence.
ACCESSION   DACBZN010000234 DACBZN010000000
VERSION     DACBZN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.243"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            141..398
                     /locus_tag="IVD64_002966"
     CDS             141..398
                     /locus_tag="IVD64_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150161.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IVD64_002967"
     CDS             complement(630..1043)
                     /locus_tag="IVD64_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP6150162.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaaa
//
LOCUS       DACBZN010000235         1294 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.244, whole genome shotgun sequence.
ACCESSION   DACBZN010000235 DACBZN010000000
VERSION     DACBZN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.244"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..1244
                     /locus_tag="IVD64_002968"
     CDS             <1..1244
                     /locus_tag="IVD64_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6150163.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACBZN010000236         1276 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.246, whole genome shotgun sequence.
ACCESSION   DACBZN010000236 DACBZN010000000
VERSION     DACBZN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.246"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            35..532
                     /locus_tag="IVD64_002969"
     CDS             35..532
                     /locus_tag="IVD64_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6150164.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IVD64_002970"
     CDS             complement(569..1252)
                     /locus_tag="IVD64_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150165.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          217 c          224 g          391 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttcgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACBZN010000237         1273 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.247, whole genome shotgun sequence.
ACCESSION   DACBZN010000237 DACBZN010000000
VERSION     DACBZN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.247"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            23..>1273
                     /locus_tag="IVD64_002971"
     CDS             23..>1273
                     /locus_tag="IVD64_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6150166.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          394 a          255 c          248 g          376 t
ORIGIN      
        1 ccaattcata ggaggattca agatgtctca tactcagctt atcaaagata ccctaaatat
       61 tctagaccta aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca
      121 aatttgcttg atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct
      181 ttgtgtcgtc ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa
      241 tgatgtgtct gagtatcgca cttatctgga gctgaagaaa caacgattta aatgtaagtc
      301 ttgccaaaga acattcgtag cggatacctc tgttgctgaa aaacactgtt tcattagtca
      361 aaaagttcgt tggtctgtca ttgctcgctt aaaagagaat acttccatga cggaaatcgc
      421 tcgtcagaaa aatatttcca cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc
      481 attgaatcct ttcaaacaga ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt
      541 tcgaagtgtt gccagtgcaa tgagttttat catgatggac ggacaaagcc atgcattatt
      601 ggatattgtc gaaaatcgac gcttgcctta tttagaacgc tatttttctc ggttttcatt
      661 agcgactcgt gaagccgttc aattgattgt tattgatatg tatgcgcctt atgtttcgtt
      721 ggtgaaaaag cttttcccga atgctcagtt gatcatcgat cgtttccaca tcgtccaaca
      781 tattggcaga actttcttga atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa
      841 ttcccactct caacgagggt tagggaaaaa attgaagcgc tattggaaac tcctacaaaa
      901 agaagaagca aagttaaact atgaaaaacg ggtttggcgt gccagtttta aagattattt
      961 gactgaaagt gagattgccg atcgactgtt agctgcttgt cccaacttac gccaaggcta
     1021 tcagttatac caagacattc tttatgcggt aaagaaaaga gatcagtcac tatttgagga
     1081 ttgtttaact agagacatta gcggtctgcc agaaacctat acaaccactt tacgaacgtt
     1141 taaaaagttt ctgccacaga tccaaaatgc gcttcactac agctattcaa acggaccatt
     1201 ggaatgctta aacaaccaca tcaaagtatt aaaacgcaat gcgtacggct ttcgcaactt
     1261 ttacaatttc aaa
//
LOCUS       DACBZN010000238         1268 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.248, whole genome shotgun sequence.
ACCESSION   DACBZN010000238 DACBZN010000000
VERSION     DACBZN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.248"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..903
                     /locus_tag="IVD64_002972"
     CDS             <1..903
                     /locus_tag="IVD64_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6150167.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..>1268
                     /locus_tag="IVD64_002973"
     CDS             916..>1268
                     /locus_tag="IVD64_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP6150168.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAV"
BASE COUNT          413 a          193 c          293 g          369 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgt
//
LOCUS       DACBZN010000239         1266 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.249, whole genome shotgun sequence.
ACCESSION   DACBZN010000239 DACBZN010000000
VERSION     DACBZN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.249"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(173..1030)
                     /locus_tag="IVD64_002974"
     CDS             complement(173..1030)
                     /locus_tag="IVD64_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6150169.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          362 a          254 c          184 g          466 t
ORIGIN      
        1 tctgtctttc aacattggag gaagttttta tttcttgaac ttggtgcact tctaattgct
       61 tattttcaga tggtgtaatt gcttgctgct ttttctttgc ttctgcttct ttttttattt
      121 tggcgagtcc tttgttgttc ccaaaaatat caagtccttt gatgtcagcc aattactcca
      181 cctccgcata ccaattctcg tgttcttcca attctttcaa aatactgata aataattcgt
      241 ggcttttgcg atcccattgt tttaagagac ctttctttgt cttctccatc gtaatacctg
      301 atccgtcgta tcgttttaag cgcgcatttt tgagtacaac atttttaatc aaatgttccc
      361 cataaatctc ttgggcttgt ttatacattt cctgatctac tgcgtcaccg ctttccaaca
      421 tgaaaggaat aacaccagca acatataaat ttgtatcaaa ttctttcaca aaaaagtcca
      481 tatactcaag atattgcatc gcatttctca tcgattttac ttgggtctgt aagacgacga
      541 ttgcccaatc cgcaatgtac atcgcggatt tactatatac agaaatagaa ggtgggacat
      601 ctaaatagat gacgtcaaac tcatcaaata atggttctag cttttctttc atatatttga
      661 attgggataa tgaatcatta tattggtctt tgatccaatc atcatacatt tcaaaggaca
      721 tgtctgctgg aagtatatag aggttattga ttactttata gacagcctct ttaggtggaa
      781 gattgtttat caacacgtca gtcacagtat tttcgtaaac aatcggttca ccttctccgc
      841 ctccttcttc gttagtgaag ttcatatgcg ttaagttcat cacatcagaa gtatttcctt
      901 gcatatccaa gtcaattatc aaggttcttt ttccttcatt aactgctgat tcaaacccta
      961 acatttgcgc gcacgtactt ttacctacgc cacctttaaa attaccgatt acatatttct
     1021 tcgctaccac gttcctacct cttttctttt agcaatattc atttgatact attataacat
     1081 atttattttt agttactacc aaaaaaaaga tatatatgta ctaccttata attaataagt
     1141 aaaaggtagt acataaataa ggaaaagctg aaatatattg atttatagct ttcctcaaag
     1201 taatgacgtt atttaattta tcgagaagca gagaatactc gcgactttag tcatgtagac
     1261 gaatca
//
LOCUS       DACBZN010000240         1258 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.250, whole genome shotgun sequence.
ACCESSION   DACBZN010000240 DACBZN010000000
VERSION     DACBZN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.250"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..1171)
                     /locus_tag="IVD64_002975"
     CDS             complement(<1..1171)
                     /locus_tag="IVD64_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP6150170.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEF"
BASE COUNT          367 a          273 c          211 g          407 t
ORIGIN      
        1 taaattctaa atattttcgt ggagaagcca gccactcagt gtctttttcc atcaaaagtg
       61 ttccaattat ccgagtgatg gaattgatat ttggaaaaat ccgaatcact cgttctctcc
      121 ggcgaatctc ttcgttcaac cgttccaaca cattggttgt acgaatcctt cggcggatat
      181 tttcaggaaa agacaaaatt tgaatagcat cttcaaagcc gttttctaaa atgtcgcaag
      241 cagcacttaa ttttgaatca caaccatatt tttccaagaa gtgctctttt ctttcacgtg
      301 tcaattcaag ttcagaggct ttaaagatac tcttcaactc gtcttttaca tcagaagaaa
      361 ctttctttgg cagcttatca aaaatatttc tcaaaaagtg ggcttggcat ctttgccagc
      421 tcgcacctaa aaagttcttg cgaacagcct taactaaccc tttgtgtgca tcagaaatga
      481 ccagttttac accgaacaaa cctcgttgaa cgaacgattg aaaaacggta tcccaattat
      541 cttccgattc gccgtcttga acatcaaaac caagaatcgt acgtcgtcca tcagaatcaa
      601 tgccgatacc aatatataca ccttttgaga caatacgatg attttccctt actttcatat
      661 acagggcatc acacatgaaa aatggatatt ccgagccttc taggctccgg tttctccact
      721 caaaaacaaa tggatccaac tgtttcatca gatttgaaac gaatgattta gaaacagtcg
      781 caccatccgt tagcagttca actgttttag ttactttgcg agtggagaca ccagaaatga
      841 ccatttcaat catggcagcg atcaaagctt tttcgcttcg ctgatatctt tcaaagatag
      901 taggactaaa cttaccgtct ctggttcgtg gaacatgaag ttctaacgta ccaattcgag
      961 ttgtaaacga acgatcgtag taaccatttc tttgtgattg ccgttcttct gaacgttcat
     1021 aggcatttgc ctgaatatat tgatttcgtt gttcttccat cagctgatta aacatgatag
     1081 tcataagtgt ttttgcggta tcattcgcac cgctttcgtt tataattgct tgaatttctt
     1141 cgctttcaaa agtaaaatgt acttgagtca ttgatatcag tcctattcga gtatgttttc
     1201 tgcaaaaaac attgtactcc taactgagat caatggcttt tttgatgctt cactttta
//
LOCUS       DACBZN010000241         1246 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.251, whole genome shotgun sequence.
ACCESSION   DACBZN010000241 DACBZN010000000
VERSION     DACBZN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.251"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            82..>1246
                     /locus_tag="IVD64_002976"
     CDS             82..>1246
                     /locus_tag="IVD64_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP6150171.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYP"
BASE COUNT          413 a          245 c          239 g          349 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atcctt
//
LOCUS       DACBZN010000242         1241 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.253, whole genome shotgun sequence.
ACCESSION   DACBZN010000242 DACBZN010000000
VERSION     DACBZN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.253"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            440..973
                     /locus_tag="IVD64_002977"
     CDS             440..973
                     /locus_tag="IVD64_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP6150172.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          392 a          230 c          230 g          389 t
ORIGIN      
        1 ttcctcatat ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
       61 ttattgagaa gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa
      121 tagttcctca tattttgcta ttttttgagc taataccgag ggtcagctga ttgaacaccg
      181 tttagtggag aagttgtgaa aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa
      241 atggttcctc acaactttgc ataaatttga cttaataccg agaagtcatg atccccatct
      301 aactccaaat cacaaggaat gagttcaaaa actagctttt ctcaacaaat attacataaa
      361 aaattctatg ttttttgtta aatttatttg cttttttcaa aagtatgtta cagtaatttc
      421 ccaaggaggt tttacagaaa tgaattttgt tgccatggat tttgaaactg ccaatcatca
      481 gccttatagt gcttgttctc ttgcgctagt catggtaaaa aatagccaga tcgttgatga
      541 attctataca ttgatacagc ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg
      601 aatccatcaa gaagacgtac gcaatgcgcc gaaattccca gaagtctggc aaactatcca
      661 accatatttt caaaaaaatc gtttagtcgt tgcacataat gctgcttttg atacaaaggt
      721 cttagcggga tgcttagatt attatcatct tgaacagcct aattatcttt ctttatgtac
      781 agtgaaaagt agccgtcgcc tgtttcctga atttccaaat catcgattga acactgtgtg
      841 cgaaaatttg aacatccaat tagatcatca ccatgatgca ttggaagata gtcgtgcatg
      901 tgcggaaatc ctgctttatc aagaaaaaaa attcgggaca gatccattga aaaaacttgt
      961 gactataaag taaaagaata cttatgaaac acacaaagag gtcgtgagcc tgccgtttca
     1021 ctccgagtca caaggagcaa tttcaaaaaa tagcttctca tgtttattaa atattgtgac
     1081 tggtgaccga ggagctggca ggagaacacc gtttattgga gaggttgtga aaaagctgtt
     1141 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg
     1201 gacttattac cgcagatgca agcttttgga caccgtttac c
//
LOCUS       DACBZN010000243         1228 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.254, whole genome shotgun sequence.
ACCESSION   DACBZN010000243 DACBZN010000000
VERSION     DACBZN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.254"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            27..1205
                     /locus_tag="IVD64_002978"
     CDS             27..1205
                     /locus_tag="IVD64_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP6150173.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          451 a          228 c          205 g          344 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc
      541 aaaccgtatc caaagaagcg atttatattg ccattggtat acgagaagac ggcactaaag
      601 aagtactgag ttatgcgatt gctccaactg aatcaacata cgtttggaat gagctgctac
      661 aggatattaa ctccagagga gttcaagaag tcttgctttt tattacggac ggcttaaaag
      721 gcatgaaaga tactatccat caaatttatc ctaaagcaaa atatcagcat tgttgtatcc
      781 atgtatctcg taacatcgct cataaagtac gtgtcaaaga ccgaaaagaa atctgtgatg
      841 actttaaggc tgtttatcaa gctaactcaa aagaagaagc gaataccttc ttatccggca
      901 tgattgagaa atggaagaaa aactatccta aagtgacgca gtcactcata gaaaaccaag
      961 acttattaac tttttatgat tttccaccta gcattcgtag aaccatttac tcaaccaatc
     1021 taatcgagtc tttcaataag caaattaaaa gatacagccg tagaaaagag cagtttcaaa
     1081 atgaagaatc actagaacgc tttctagtca gcatttttga tacatacaat caaaaatttc
     1141 taaacagaag ccataaaggt tttcaacagg taaccgatac attagtttca atgtttactg
     1201 agtaactaat tattttgcag gaggacaa
//
LOCUS       DACBZN010000244         1204 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.255, whole genome shotgun sequence.
ACCESSION   DACBZN010000244 DACBZN010000000
VERSION     DACBZN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.255"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            4..963
                     /locus_tag="IVD64_002979"
     CDS             4..963
                     /locus_tag="IVD64_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6150174.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          401 a          229 c          244 g          330 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtag
//
LOCUS       DACBZN010000245         1193 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.256, whole genome shotgun sequence.
ACCESSION   DACBZN010000245 DACBZN010000000
VERSION     DACBZN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.256"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..1076
                     /locus_tag="IVD64_002980"
     CDS             <1..1076
                     /locus_tag="IVD64_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6150175.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACBZN010000246         1174 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.257, whole genome shotgun sequence.
ACCESSION   DACBZN010000246 DACBZN010000000
VERSION     DACBZN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.257"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            31..291
                     /locus_tag="IVD64_002981"
     CDS             31..291
                     /locus_tag="IVD64_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150176.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(653..892)
                     /locus_tag="IVD64_002982"
     CDS             complement(653..892)
                     /locus_tag="IVD64_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150177.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          416 a          164 c          219 g          375 t
ORIGIN      
        1 taatggaaaa acattaagga tggatgaaat atgaataacg aaactatcat tttaggaaaa
       61 aaatatattt gccaacctat tggcttgaaa caaaaagtgg ttggagaagt aatcagtaaa
      121 ttagaaaatt gtgttgtgat ctgcgtagac gaatatcatg acgtcgatcg caatgaaata
      181 ttagaaaaat gcggaaaagt agtggcaaaa tatactcacg tttatgagtt ggaaacaaca
      241 gaatatctat ataactatag caacaaatat gaacatactt tagtttcata aagatttaaa
      301 taaagtgcct tcaaaagcga gaagaccaag cttttgaagg cactttattt agttggcagt
      361 gattgttcat gatttttgat ttattcaaaa ggttcgccta gagaatctgt ctctgtaacg
      421 aggcctggtg gagaaactcg ataaaaacct atactgccag aactgtccta ttttcggata
      481 gacaaactag tagttttaaa aagatcatcc gatccaatca tttcaaaaaa tgcaaaactg
      541 atatcaggat catttagctc aggcttgtta tcgtaaagca tttgtaaagg ttgcatttag
      601 ttaggagtaa acgcgaaact atcatcatta tttgctgaag aagatgaaga agctaattca
      661 gagtttgttt gaaataaagg agacgtgctg acagtttctt taccctgttt tttgcagagg
      721 tagaaacttt tatatcggaa gtttctaccg gagaattttt ctcatttgta gaaattgttg
      781 aatcgatact attcgaaatt tgcgtatctg aaggtatgtt ctttgatgca gaagtcccac
      841 aagcagtaag taacaataaa tttgataaac tgataaacag aattcttttc attatgtaca
      901 cactccttat agtatcgttt agcatacacc tatgctcagt attaggcaaa atcaatgcaa
      961 aactatcaag tcttttatag tatctattag tcttagtaat acatatatgg agagggtgat
     1021 atcccagttc aaaccagata cattggttac catctgtatt aacgattatg cctctaaacg
     1081 ctttttttat gaaaaaattt ataagtgaaa acgtaggtat gatattatct agaggtaatt
     1141 tttttgggta tttaaatagt tgctaggagg atag
//
LOCUS       DACBZN010000247         1134 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.258, whole genome shotgun sequence.
ACCESSION   DACBZN010000247 DACBZN010000000
VERSION     DACBZN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.258"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            55..>1134
                     /locus_tag="IVD64_002983"
     CDS             join(55..331,331..>1134)
                     /locus_tag="IVD64_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6150178.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          425 a          192 c          217 g          300 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acac
//
LOCUS       DACBZN010000248         1106 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.260, whole genome shotgun sequence.
ACCESSION   DACBZN010000248 DACBZN010000000
VERSION     DACBZN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.260"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..245
                     /locus_tag="IVD64_002984"
     CDS             <1..245
                     /locus_tag="IVD64_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150179.1"
                     /translation="DDNHQHDDYGSYSFTLGDVQTYAQTSSVKEEQNFVVFVDYTIGN
                     PQFKDVKPQKIKGGLTVTEKQVDGQWKVVDFSYFTR"
     gene            256..1062
                     /locus_tag="IVD64_002985"
     CDS             256..1062
                     /locus_tag="IVD64_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150180.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
BASE COUNT          454 a          168 c          225 g          259 t
ORIGIN      
        1 cagacgataa tcaccaacat gatgattacg gaagttactc ctttacatta ggtgacgtgc
       61 aaacttacgc tcaaacaagc agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt
      121 atacaatcgg caatccgcaa ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa
      181 ctgtcactga aaaacaagta gatggtcaat ggaaagtcgt agactttagt tatttcactc
      241 gttaggagga agaaaatgag taagaaaaaa ggagaaagac aacgtttcaa aaaacgaaaa
      301 agttatcttc aatggctggg aatctttatg attctcggct ttttgattct tgctagtccg
      361 atttatacga atcagaaggt agatatacca gatgaggtaa gcacgaacgc ctttttaaac
      421 acgaaagagc tgacaatggt ttcaaaagaa aaaggagcga accagcaatt agtttttgaa
      481 tttgcgatcg acgatgaaga tcaaagcttg ttaaaagaac tggctaatct taattatcgc
      541 gtagaagtaa agacagctaa aggggattat caagcaatac gagccaaagt gatcaaggtg
      601 tctgatgatt attttgcagt gaagctaacg catgtaccag aagaatatat cgccatgcgt
      661 ttaaccatta ttcctgaaaa aattgatcca aaagtggata tgcaagaacc acaagatctg
      721 atttattacg tgcacgaaga taaggttaaa gatcaagtga aagatgaaga ttatgaacaa
      781 cacgcgatca attacaaagt gaaaggatac aagaaagaac aaaaagcagc tcaaaaacaa
      841 atggaatctt tccaagcaac cattgatttg aatgaagaat tagtgaagaa gctaaaagat
      901 caattgcctt atcaagtcgc agatgatcag gaaaatactg aaaacaagat taatagctac
      961 cagcaagaaa ttgaaacatc gaaaacgcaa ctgaaagatc aagaagagat cattcaaaaa
     1021 cttcaagaaa aaattgatcg tatgacaaaa gaaaagattt agaaggacag gtggagtgaa
     1081 aaaatgaagc gaacaaactt ggctga
//
LOCUS       DACBZN010000249         1089 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.261, whole genome shotgun sequence.
ACCESSION   DACBZN010000249 DACBZN010000000
VERSION     DACBZN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.261"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..276
                     /locus_tag="IVD64_002986"
     CDS             <1..276
                     /locus_tag="IVD64_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6150181.1"
                     /translation="RNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQ
                     GGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            619..875
                     /locus_tag="IVD64_002987"
                     /pseudo
     CDS             619..875
                     /locus_tag="IVD64_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          376 a          164 c          198 g          351 t
ORIGIN      
        1 cgtaatccct tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc
       61 caagaaaaag ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa
      121 tatttgtcgc aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt
      181 catgctagaa ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt
      241 gaaaaaagag aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc
      301 taatgtgagc tatgccttct atataagact aagttctatt ataagtaacg aagtaattct
      361 cgttccttcc tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct
      421 ccagtaaatt ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt
      481 agttggctac ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat
      541 ttactacaag gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa
      601 aatcacatca ggaaatgcat gagattcttt aagaaaggca attatagggc tatgattacg
      661 gttcgtgtaa gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt
      721 actttatctg aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt
      781 caaacggctg aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg
      841 gaagaatcct taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga
      901 aattcagaag cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat
      961 tcgttgttac cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt
     1021 atttttacaa aaaaataata actggaatct agttaaaact cttgaagtga gagatttgac
     1081 tagattttt
//
LOCUS       DACBZN010000250         1082 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.262, whole genome shotgun sequence.
ACCESSION   DACBZN010000250 DACBZN010000000
VERSION     DACBZN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.262"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(48..132)
                     /locus_tag="IVD64_002988"
     tRNA            complement(48..132)
                     /locus_tag="IVD64_002988"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Leu,seq:caa)
     gene            complement(153..223)
                     /locus_tag="IVD64_002989"
     tRNA            complement(153..223)
                     /locus_tag="IVD64_002989"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(189..191),aa:Cys,seq:gca)
     gene            complement(269..340)
                     /locus_tag="IVD64_002990"
     tRNA            complement(269..340)
                     /locus_tag="IVD64_002990"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Gln,seq:ttg)
     gene            complement(352..424)
                     /locus_tag="IVD64_002991"
     tRNA            complement(352..424)
                     /locus_tag="IVD64_002991"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(389..391),aa:His,seq:gtg)
     gene            complement(427..500)
                     /locus_tag="IVD64_002992"
     tRNA            complement(427..500)
                     /locus_tag="IVD64_002992"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Trp,seq:cca)
     gene            complement(507..587)
                     /locus_tag="IVD64_002993"
     tRNA            complement(507..587)
                     /locus_tag="IVD64_002993"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Tyr,seq:gta)
     gene            complement(589..664)
                     /locus_tag="IVD64_002994"
     tRNA            complement(589..664)
                     /locus_tag="IVD64_002994"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(629..631),aa:Phe,seq:gaa)
     gene            complement(700..772)
                     /locus_tag="IVD64_002995"
     tRNA            complement(700..772)
                     /locus_tag="IVD64_002995"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(737..739),aa:Asp,seq:gtc)
     gene            complement(782..854)
                     /locus_tag="IVD64_002996"
     tRNA            complement(782..854)
                     /locus_tag="IVD64_002996"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(819..821),aa:Val,seq:tac)
     gene            complement(870..941)
                     /locus_tag="IVD64_002997"
     tRNA            complement(870..941)
                     /locus_tag="IVD64_002997"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(906..908),aa:Glu,seq:ttc)
     gene            complement(956..1045)
                     /locus_tag="IVD64_002998"
     tRNA            complement(956..1045)
                     /locus_tag="IVD64_002998"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1009..1011),aa:Ser,seq:gga)
BASE COUNT          303 a          283 c          247 g          249 t
ORIGIN      
        1 ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac cggcggccgg
       61 ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt ctgccaattc
      121 cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg aaccggtgat
      181 gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac tattatcaat
      241 ctataaaata aagaataaat tatagaaact ggggtagctg gattcgaacc aacgcatgag
      301 ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa gggcgaatga
      361 tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat
      421 tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag accgctgttc
      481 tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac tgaaccctaa
      541 ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg gtggcgcggg
      601 acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac tgagctaccg
      661 cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg ggaatcgaac
      721 ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa ccagttcatt
      781 atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc
      841 tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac ccgtgttacc
      901 gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt tgctacggag
      961 agtaagggat tcgaaccctt gagacagttt ggcaccatct acatgatttc caatcatgct
     1021 ccttcggcct ctcggacaac tctccagagt gaaagaagct caatactctt tctcaaaatg
     1081 ag
//
LOCUS       DACBZN010000251         1067 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.263, whole genome shotgun sequence.
ACCESSION   DACBZN010000251 DACBZN010000000
VERSION     DACBZN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.263"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..969
                     /locus_tag="IVD64_002999"
     CDS             <1..969
                     /locus_tag="IVD64_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6150182.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          367 a          185 c          214 g          301 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcaccc
//
LOCUS       DACBZN010000252         1058 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.264, whole genome shotgun sequence.
ACCESSION   DACBZN010000252 DACBZN010000000
VERSION     DACBZN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.264"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(36..992)
                     /locus_tag="IVD64_003000"
     CDS             complement(36..992)
                     /locus_tag="IVD64_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150183.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACBZN010000253         1050 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.265, whole genome shotgun sequence.
ACCESSION   DACBZN010000253 DACBZN010000000
VERSION     DACBZN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.265"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            63..881
                     /locus_tag="IVD64_003001"
                     /pseudo
     CDS             63..881
                     /locus_tag="IVD64_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          380 a          151 c          212 g          307 t
ORIGIN      
        1 agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc gcaacagaac
       61 cgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat ggtaagttaa
      121 aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc cgtatcgagt
      181 acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact tatgatgatt
      241 acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt ttggaagaac
      301 aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca gaaaaagagg
      361 gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg tatgatattg
      421 attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca gatgcacgag
      481 aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa gaagtttacc
      541 gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat atctttgtca
      601 ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt tccaatgagt
      661 ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca ggtgaagatc
      721 caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat aaagccaaac
      781 atccagaagc gaaaccattt aaatacccac aagaaatgaa gcgagcatgg cgtaaaatgt
      841 tagatgatga gttgattcca cttgaaaatg aattgaggta aacgagaaga gttaagaaaa
      901 ttgtacgatt caaagatagt agtaaagtgg aagtgtagaa aatagggata gatgtctttt
      961 tacttgttca cattttataa cggagtattt aagaaagcta ttgaataatg attcttccat
     1021 ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DACBZN010000254         1049 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.266, whole genome shotgun sequence.
ACCESSION   DACBZN010000254 DACBZN010000000
VERSION     DACBZN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.266"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            4..978
                     /locus_tag="IVD64_003002"
     CDS             4..978
                     /locus_tag="IVD64_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6150184.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          375 a          132 c          182 g          360 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg taggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacattttt
//
LOCUS       DACBZN010000255         1021 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.267, whole genome shotgun sequence.
ACCESSION   DACBZN010000255 DACBZN010000000
VERSION     DACBZN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.267"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..513
                     /locus_tag="IVD64_003003"
     CDS             <1..513
                     /locus_tag="IVD64_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP6150185.1"
                     /translation="EDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWR
                     EGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLY
                     QKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQN
                     VFTLEKVGVI"
     gene            513..>1021
                     /locus_tag="IVD64_003004"
     CDS             513..>1021
                     /locus_tag="IVD64_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150186.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          360 a          129 c          230 g          302 t
ORIGIN      
        1 gaagatttaa ttgctgatat gacagatttt attaatgaaa ataaaggcga ttttacggac
       61 tggatgaatg aaaggaaaga agaatttgat cgctggagag aagaacagga gaatagtttc
      121 caagattgga gagaaggaca agaatcagat tatttgtcat ggtttgaatc aatcaaggat
      181 attttgaagt ctatcgatcc aggtggagta atgttagccg aattaatgga tgcacgtgta
      241 gatcttcaag gtgtacgcca tgaatcgatt tctgaacgtt tcctatccga tctaaattat
      301 ttgtatcaaa aaatgaaagc agcacttttc acgattgaat acggtgaaat tgaagtgact
      361 gatattcttc aagacgatct cttttcagat aatcacgaag tcgaaaaaat tgaaacagta
      421 gaatttccaa ttgaagaagg agctttgatt attgcaactg ttgatgatcc aaaacagaat
      481 gttttcactc ttgagaaagt tggggtgatt tgatggttaa agtaaagaga atgatggaga
      541 ctgatgaatc tggtgttaaa cgtcaattta tgccaattac acatgtttct gcagttttag
      601 gcttagaaaa aatgatatca ggacaatcaa aagttctttc ggttaatggt aaatatggtg
      661 cagtcatttt aacgaaagct gatttaggat tagagaatgc aattactgaa ctaccttatg
      721 caagcgagac aagcgatggt attcttactg ctgaaatgtt tcaaaagatc gtaaatggag
      781 aaggtggaac atatattttg ccgattgcta ctcctgaaca attaggaggt ataaaagttg
      841 gggaactctt agaagttact gaagaaggtg ttttatctgc aactaagcaa acagacttca
      901 atttctctga agaattaaag ggaaaattag aatctctaaa aatattaaaa gcaggtgcaa
      961 atatatcaat tgcagaagat ggaacaatta gttctacagg tggtagtggg actggtggtg
     1021 t
//
LOCUS       DACBZN010000256         1002 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.268, whole genome shotgun sequence.
ACCESSION   DACBZN010000256 DACBZN010000000
VERSION     DACBZN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.268"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            52..307
                     /locus_tag="IVD64_003005"
                     /pseudo
     CDS             52..307
                     /locus_tag="IVD64_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            371..1002
                     /locus_tag="IVD64_003006"
                     /pseudo
     CDS             371..1002
                     /locus_tag="IVD64_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          344 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaaaa
      301 tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt cccattgtta
      361 ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga agccgtcaaa
      421 aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt caattgtgga
      481 taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata ttttgcctac
      541 acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg aagcggtgga
      601 atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg tcttaccaaa
      661 tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg cgttcaatga
      721 ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg aagccgagaa
      781 agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc actatgatta
      841 caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag caatgatgat
      901 tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt tccatgagct
      961 gaccgaagcc tttagagaca aggaatcaga cttatttttt ac
//
LOCUS       DACBZN010000257          993 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.269, whole genome shotgun sequence.
ACCESSION   DACBZN010000257 DACBZN010000000
VERSION     DACBZN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.269"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            52..>993
                     /locus_tag="IVD64_003007"
     CDS             52..>993
                     /locus_tag="IVD64_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6150187.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDD"
BASE COUNT          356 a          180 c          190 g          267 t
ORIGIN      
        1 gtgctacact caaaatagtt ccgacgaaag aattataagg agtgagtaca aatgacctat
       61 aaccatctta caccaactga actcgtaatg atagaagctt atttcaatca atcacaacct
      121 gtttcaaaag tagccaatct gttacaacgt tctcgacaaa ctatttataa ggtctatcgt
      181 tttcttaaat ctggtggaag cgcaatgagc tactataaac aatataagaa aaacaaacgt
      241 aactgcggaa gacatccgat tgttttacct gatgagcaac aggaatacat tcaaaaaaag
      301 gttgttcaag ggtgggctcc tgatgtcctt attggtcgag cagagtttcc tattgcttgt
      361 tcggtacgca cactgtatcg aatgtttaaa gataaaacct ttgacactca cgatttacca
      421 atgaagggaa aaagaaaacc aaacggtcat aaagaaaaaa gaggaaaaca agggtttcgc
      481 cggtcaatcc gagacagaaa gacagactat acccagtttg atcatgaatt tggtcacctg
      541 gaaggtgaca ctatcgtggg tggaaaacat aaaagcgcgg tcatcacatt agttgaacgg
      601 ctatctaaag tcatcattac cttgaaaccc gaaggcagac aggctaaaga cattgaaaat
      661 aggctgaatc aatggtttcg ttgtgtccca aaaaacttat ttaaatccat cacatttgat
      721 tgtggaaaag aattctcaaa ttggaaaaag attagtaata ccaacgatat ttcaatttat
      781 tttgcggatc caggaacacc ttctcaacga ggattgaatg aacattcaaa tggcttatta
      841 cgtaaagatg gtttgtataa agctatggat tttaatggtg tatctgaaac ctttatccaa
      901 tcggtggcat cgaaacgaaa tcatattcct agaaaatcat tacactatcg gacaccaatg
      961 gaagtatttt tgagttacgt cacaaaagat gat
//
LOCUS       DACBZN010000258          978 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.270, whole genome shotgun sequence.
ACCESSION   DACBZN010000258 DACBZN010000000
VERSION     DACBZN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.270"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..337
                     /locus_tag="IVD64_003008"
     CDS             <1..337
                     /locus_tag="IVD64_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150188.1"
                     /translation="KNYSEAVRFLCMNYDPEVEKKVNSNQAKLNGMSKEISILTEMVS
                     EFADLHLKDTGILIGKNSTVYQEAKERVEGKIKSNQTKKYSPQLKKPVNESVHARVDP
                     FDPTKILKR"
     gene            356..>978
                     /locus_tag="IVD64_003009"
     CDS             356..>978
                     /locus_tag="IVD64_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150189.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTL"
BASE COUNT          389 a          150 c          182 g          257 t
ORIGIN      
        1 taaaaactat agtgaagctg ttcgcttttt atgtatgaac tatgatccag aagtggagaa
       61 aaaagtaaat tccaatcaag caaagttgaa tggaatgagt aaagaaattt ctattttaac
      121 cgaaatggtt tcagaatttg cggatcttca tttgaaagat acaggaatat taattggtaa
      181 aaattcaact gtttatcaag aagcaaaaga acgagtggaa ggaaaaatta aatccaatca
      241 aacaaaaaaa tattcccctc agttgaagaa acctgtgaat gaatctgttc atgccagagt
      301 agatccattt gatccaacga agattctaaa aagataaaaa tgaaggagga taataatgac
      361 tcaatcgcct tcagttattc tcacttgtca atttactttg ccgaataaaa agtcattttc
      421 tacttatatt gactatatta caagggaaca ggctcttgaa gaaaaggaac gacgtacgcc
      481 cgaagaagaa caggaacttc aacgaatcaa gaatgcttta gaggagtttt atattcctga
      541 aggagaaact ttttcggaga gtaagacggg taaagaattc agtgaaacgt cgaatgaagc
      601 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
      661 ttatctttct tatatgacac gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
      721 tgaacaaaaa gaataccaac gcttgaacca agcaatgaaa aaatatcaaa cagaacccca
      781 taaaaaagct tcaggaaatc ttcctggagt tttttctata gcttcagacg aagtgaaagg
      841 aagtgatctg aaggagatta agcaaatctt ccaaagagga caacaaaaag gctcaatcat
      901 ttatcaagat gttgtttctc atgataattc gtatcttgaa aaaataggat tgtacgatcc
      961 caaaacagat actttaga
//
LOCUS       DACBZN010000259          958 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.272, whole genome shotgun sequence.
ACCESSION   DACBZN010000259 DACBZN010000000
VERSION     DACBZN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.272"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..166)
                     /locus_tag="IVD64_003010"
     CDS             complement(<1..166)
                     /locus_tag="IVD64_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6150190.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANET"
     gene            complement(163..552)
                     /locus_tag="IVD64_003011"
     CDS             complement(163..552)
                     /locus_tag="IVD64_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6150191.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(652..792)
                     /locus_tag="IVD64_003012"
     CDS             complement(652..792)
                     /locus_tag="IVD64_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6150192.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
BASE COUNT          274 a          206 c          130 g          348 t
ORIGIN      
        1 cagtttcgtt tgctgtttca tgcaatagga tgtattggtt atttgctact tgagaactac
       61 cttcgtttgc gcccaaattg aattcattgt tgatcgagta agcaaaggcg tttattggca
      121 ataaaaaaag agctataata gctcctacaa agattttctt tttcatttgg ttcctcctcg
      181 gtctttattt tcagtatcat ttcgtatttg ttggaatacc tcttttaact tatctggcaa
      241 aggaaggaat accgaaatgt tttctaaaaa actaatccct tcatttgcaa catagaacat
      301 aataactatt tctcttagag gtatagaatt cccaacgatt ttctgtacgt caaccgcaac
      361 ggcaatcaca acgaagatta agactttctt caacaatcct tcgaaaccta tctcactact
      421 tatttctttc aatttccatg ccttagcaag tccagttaag aagtccacac ccacaagaaa
      481 aattaaaaca tgtagtatat tgtccatccc tcccaataaa ccaacaattg tcccgcctat
      541 cacccctgtc attactgaga gtgtatttaa atatttttcc aatatctatt tctcctttat
      601 gcaaagaagc gcactcgttt gagtacgctt tttatttaac tttgtgctta attaaatttc
      661 atttccagta atctgcttat attggtcttt tgttaaacag ttcatgttta catagtcaag
      721 taaatcttgg tccgtataac atccccaatc ataaaattgt tttatatttt caaagcccgg
      781 aaaactattc attacttttc cctccattct taatacttgc gacttccatc gtcaattgag
      841 ctaacatttg ttgcattttt tcttgttcct gtaccgatat cgccaattgt tttgtcagta
      901 gagcatttaa ttgcttcaac tgatcgacat cttcaatatc gtcaaccgct gtgccact
//
LOCUS       DACBZN010000260          943 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.273, whole genome shotgun sequence.
ACCESSION   DACBZN010000260 DACBZN010000000
VERSION     DACBZN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.273"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..258
                     /locus_tag="IVD64_003013"
     CDS             <1..258
                     /locus_tag="IVD64_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150193.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="IVD64_003014"
     CDS             260..397
                     /locus_tag="IVD64_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6150194.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            434..727
                     /locus_tag="IVD64_003015"
     CDS             434..727
                     /locus_tag="IVD64_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150195.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            724..>943
                     /locus_tag="IVD64_003016"
     CDS             724..>943
                     /locus_tag="IVD64_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6150196.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          350 a          134 c          201 g          258 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccatta tgcattacag acgaagaagc
      361 agataaaatc attaataaag ataagagcgc atcttaattg atgcgttttt atttaaggta
      421 aaggagttgt cacatgatta atttagggga atggggaatg atagcaggat caataaccgc
      481 tatcgtttct ttgattttat tagtaattag accgattgct gcatctttct cgaaaattac
      541 tgagactctt tcaaaagtaa gccacaattt agatttgctg actaaagatt tagaatcgag
      601 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
      661 acatacagaa aaattggtgg aacacacgca acaaatcaaa actttgttta gggagagaag
      721 aaaatgaata ataaaacgtt cgaagtacta aaatggttcg cactggtaat tattcccgca
      781 ctagctactt tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac agatatctgt
      841 gttgtcatca ttactggttt tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga
      901 acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa aca
//
LOCUS       DACBZN010000261          943 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.274, whole genome shotgun sequence.
ACCESSION   DACBZN010000261 DACBZN010000000
VERSION     DACBZN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.274"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            2..>943
                     /locus_tag="IVD64_003017"
     rRNA            2..>943
                     /locus_tag="IVD64_003017"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACBZN010000262          919 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.275, whole genome shotgun sequence.
ACCESSION   DACBZN010000262 DACBZN010000000
VERSION     DACBZN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.275"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..550
                     /locus_tag="IVD64_003018"
     CDS             <1..550
                     /locus_tag="IVD64_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150197.1"
                     /translation="FIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKN
                     FLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDF
                     GKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGIG
                     KLRLDGSNIQQNKPYVLGKVVI"
     gene            594..863
                     /locus_tag="IVD64_003019"
     CDS             594..863
                     /locus_tag="IVD64_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150198.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT          344 a          123 c          178 g          274 t
ORIGIN      
        1 atttatcagg caaaatttaa caatgagtaa acttttcaaa agtttattgg acttacttat
       61 cttaggcagt atgttaacga ctctaacaga tcggttcgaa caatctgaaa aaacggtaga
      121 ggggataaag aacttcttaa cgacgattga tcaatttata aatggatata tgtatttgtt
      181 tgaagtaggc tttattctca ctttgtttgg ttgtgtattt ataaaactca ttaaaagatc
      241 aatcgaaaaa gaaatggtta ttgatctaat tccgacgctg agcggctatg agtattacgt
      301 tgattttgga aagtatgagg agttgatcca tacggatatc gttcacacga gtatccatga
      361 agtaaccgtt aagggcatca gatctacttt aaaagatgga caggagacga aaacaatcga
      421 ccgaagaaaa gaggtctaca atttttataa agttgaagca agtctacgat atggcattgg
      481 gaaattgagg ttagatggga gtaatattca acaaaacaaa ccatatgttt taggaaaagt
      541 ggtgatttag aacttcctat gaaagatgaa taaataagga gaatcttttg aaaatgattc
      601 ttctttacag aattgtgctt atagttggca cggagttaaa aatcttaaag aaggagcagt
      661 tattcaaagt gaaagaacat cataatcagt tactatgtat taaaacgggt acgatgaaca
      721 ttcaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg tcgattcgag
      781 agcaacataa gcaagaaaaa ttaatggcta cttatagcca attgattgat gagtgggaac
      841 aattcaagga atctaaaaca taaaattcca tttaaacacc ttatttttta aagagcataa
      901 ggaaacgtga ccccacttt
//
LOCUS       DACBZN010000263          910 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.276, whole genome shotgun sequence.
ACCESSION   DACBZN010000263 DACBZN010000000
VERSION     DACBZN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.276"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..355
                     /locus_tag="IVD64_003020"
     CDS             <1..355
                     /locus_tag="IVD64_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150199.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="IVD64_003021"
                     /pseudo
     CDS             615..910
                     /locus_tag="IVD64_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DACBZN010000264          880 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.277, whole genome shotgun sequence.
ACCESSION   DACBZN010000264 DACBZN010000000
VERSION     DACBZN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.277"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..734)
                     /locus_tag="IVD64_003022"
     CDS             complement(<1..734)
                     /locus_tag="IVD64_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6150200.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGT"
     gene            complement(734..871)
                     /locus_tag="IVD64_003023"
     CDS             complement(734..871)
                     /locus_tag="IVD64_003023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150201.1"
                     /translation="MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGG
                     K"
BASE COUNT          261 a          202 c          153 g          264 t
ORIGIN      
        1 gtgccgttgt tattgccacc ttcaccccaa tgtttgatgg cgaattcatc atattcggtt
       61 aatttattag aagtgataat tgacatacaa cctgtataat acggactatc ttccgtaatt
      121 cctgcagcgg cataaccgcc tcgtcctaag atcaataagc ttgcgccagg gcttttattt
      181 cctacacaag cattaaaatt ggattgtgtg gctaccataa agccgtaata ggaaaatgca
      241 gcttttgagt tgggaaactt catgtaccag ccaccttcaa tgccaccaat tcctcgtgca
      301 gttcctttag gaaacagaca accatcaaac caagtaatcc caaaatagtt gagatcattt
      361 cttgcggacg ccgatgtacc aaacgctgat tcgtaaccgt attgcgaaag gatcacactt
      421 ggtaataatt tatatttcac tgctgcacct acggcatatt gcgcaatctc atcagaaaaa
      481 gggcctcttc ctttgatttc tttataaatc ggtaagcctt ccgaatattc tcctgtaaaa
      541 gcttcgccac cactgcttga atctgaatcc gaattatcaa tcgagcccgc cgcaagtatt
      601 agaaataggg tcacgaacac agtgatcaaa atagtaatca aacctgcaga agtaccaaac
      661 aaccacaaaa ataagcgccg ctttgcacga cgcttaacat actgcttgat taaactttca
      721 ttttggtttt ccattatttt cctccatcaa acaattttaa ttgacgtttg ttcggactaa
      781 aatgaacacg aatggtttct tttgagccaa aattgatcac agcgtctcct tgattttgtt
      841 gaggaatgga ttctaattct gagttcgtca ttgatttacc
//
LOCUS       DACBZN010000265          841 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.278, whole genome shotgun sequence.
ACCESSION   DACBZN010000265 DACBZN010000000
VERSION     DACBZN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.278"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            4..>841
                     /locus_tag="IVD64_003024"
     CDS             4..>841
                     /locus_tag="IVD64_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6150202.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLY"
BASE COUNT          307 a          141 c          160 g          233 t
ORIGIN      
        1 taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat caatgatgat
       61 ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc cgaagctgct
      121 ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg tacggatact
      181 cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa tctaaaatac
      241 aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta tttaggaaag
      301 aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc tgaatttaaa
      361 aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa tgcgctgaat
      421 atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt aaaaatacca
      481 aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct tgaggacttt
      541 gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt gaatattgga
      601 ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga tgtcgatttt
      661 tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc atgggtatat
      721 ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac cttgctagca
      781 attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg aaagctctac
      841 a
//
LOCUS       DACBZN010000266          833 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.279, whole genome shotgun sequence.
ACCESSION   DACBZN010000266 DACBZN010000000
VERSION     DACBZN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.279"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..673
                     /locus_tag="IVD64_003025"
     CDS             <1..673
                     /locus_tag="IVD64_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150203.1"
                     /translation="EKRNLLTRKSDLRNTLTKVVKEPARYDRHAMNLLNEVYKTLPDK
                     RSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQISKALKEEAKSLYGESK
                     RESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVDQMQSIYNERSFRTLKQL
                     PTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEKYHASKAYEEQQRRIQM
                     EQERQ"
BASE COUNT          360 a          132 c          168 g          173 t
ORIGIN      
        1 ggagaaacga aatttattga cgcggaaaag tgatttgcgt aacaccttaa caaaagtggt
       61 aaaagagcca gcgcgttacg atcgacacgc gatgaactta ttaaatgaag tatataaaac
      121 cctacctgac aaacgttcgg aatggaatta cggggatgaa aaacgaacca aactttcacc
      181 atacacaaga gagaaactcg atgagttaac caaatatgtt ttaacaaaag aacctgaata
      241 tcaagaatat atacagattt caaaagcatt gaaagaagaa gcaaaaagtc tttatgggga
      301 aagcaagaga gaatcaaagg atgcagagaa aaatgctctc tttgatttaa aaaagcgcac
      361 aggaaatgcc atcctaacag agctgaagag acaggatagt caaataaaac cattaataga
      421 aagtgttgat cagatgcaat ctatttacaa tgaacgatcc tttcgaacgc ttaaacaatt
      481 gccaacgaat gaatacaaat atagttacga agaattcatg aagcgcagga aacaattcca
      541 acaaagagtt gtcacgcagc ggagtttgcg tcaattaaat cgaacgatta atgcccatga
      601 agaaaaatac cacgcaagta aagcatacga agagcaacaa cgaagaattc aaatggaaca
      661 agaaagacag taaagacagt aggaggaata gcaatgaaac aatatttaga tcaatggaaa
      721 gttatagaag gatcattaag agaagaacga atcgaacaac ttcctgattg tttagaaaaa
      781 gagcatttgt tccagataag agaaatgttg agaaacgaac agtttgatcc aaa
//
LOCUS       DACBZN010000267          792 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.280, whole genome shotgun sequence.
ACCESSION   DACBZN010000267 DACBZN010000000
VERSION     DACBZN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.280"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..782
                     /locus_tag="IVD64_003026"
     CDS             <1..782
                     /locus_tag="IVD64_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6150204.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          243 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc tt
//
LOCUS       DACBZN010000268          792 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.281, whole genome shotgun sequence.
ACCESSION   DACBZN010000268 DACBZN010000000
VERSION     DACBZN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.281"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            10..>792
                     /locus_tag="IVD64_003027"
     CDS             10..>792
                     /locus_tag="IVD64_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6150205.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT          269 a          137 c          153 g          233 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata at
//
LOCUS       DACBZN010000269          753 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.282, whole genome shotgun sequence.
ACCESSION   DACBZN010000269 DACBZN010000000
VERSION     DACBZN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.282"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..>753)
                     /locus_tag="IVD64_003028"
     rRNA            complement(<1..>753)
                     /locus_tag="IVD64_003028"
                     /product="23S ribosomal RNA"
BASE COUNT          163 a          236 c          176 g          178 t
ORIGIN      
        1 actaaggaca gctcctctca aatttccaac gcccgcgacg gatagggacc gaactgtctc
       61 acgacgttct gaacccagct cgcgtgccgc tttaatgggc gaacagccca acccttggga
      121 ccgactacag ccccaggatg cgacgagccg acatcgaggt gccaaacctc cccgtcgatg
      181 tggactcttg ggggagataa gcctgttatc cccagggtag cttttatccg ttgagcgatg
      241 gcccttccat gcggaaccac cggatcacta agcccgactt tcgtccctgc tcgacttgtt
      301 ggtctcgcag tcaagctccc ttctgccttt acactcttcg aatgatttcc aaccattctg
      361 agggaacctt tgggcgcctc cgttaccttt taggaggcga ccgccccagt caaactgccc
      421 atctgacact gtctcccacc acgattagtg gtgcgggtta gagtggccat aacgcagggg
      481 tagtatccca ccagcgcctc catcgaaact agcgttccga tttctacggc tcctacctat
      541 cctgtacatg cggtacagac actcaatatc aaactacagt aaagctccat ggggtctttc
      601 cgtcctgtcg cgggtaacct gcatcttcac aggtactaaa atttcaccga gtctctcgtt
      661 gagacagtgc ccaaatcgtt acgcctttcg tgcgggtcgg aacttacccg acaaggaatt
      721 tcgctacctt aggaccgtta tagttacggc cgc
//
LOCUS       DACBZN010000270          753 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.283, whole genome shotgun sequence.
ACCESSION   DACBZN010000270 DACBZN010000000
VERSION     DACBZN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.283"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            363..731
                     /locus_tag="IVD64_003029"
     CDS             363..731
                     /locus_tag="IVD64_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6150206.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          244 a          117 c          157 g          235 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt ttt
//
LOCUS       DACBZN010000271          742 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.284, whole genome shotgun sequence.
ACCESSION   DACBZN010000271 DACBZN010000000
VERSION     DACBZN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.284"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            178..381
                     /locus_tag="IVD64_003030"
     CDS             178..381
                     /locus_tag="IVD64_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP6150207.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="IVD64_003031"
     CDS             381..731
                     /locus_tag="IVD64_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6150208.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           79 c          120 g          243 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta ta
//
LOCUS       DACBZN010000272          716 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.285, whole genome shotgun sequence.
ACCESSION   DACBZN010000272 DACBZN010000000
VERSION     DACBZN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.285"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..567
                     /locus_tag="IVD64_003032"
     CDS             <1..567
                     /locus_tag="IVD64_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150209.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          266 a          124 c          157 g          169 t
ORIGIN      
        1 gaagaacaat tgtatttgga aagtgaaacg agtcaaacga aggtcacagt cagccataat
       61 cgtttagacg tgtataaacg tcgtcctgaa acggtaaaaa aaggaacgga gcttctacga
      121 ctagaaacag aaaaatacat catgaatgtt tacctagaaa ggagtgagct ggtcgaaaac
      181 tttgtcagct acacgatttg caggaaacac tattactcag aatcagaata ttattcaatt
      241 tttgagagca tggaattaat ggaagaagaa atggaagagc taaacagcaa agatgcgacg
      301 atagacgatt attaccttgt atttactaga tttttgacgt gtggaacgga atctatcgtg
      361 ttaatgggaa tggattatga actcgctgta cccgaagcat ttttgaggga atatgtagaa
      421 gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt agacacctat
      481 acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat gccatatcaa
      541 cgatttgaaa ggaaaaaaag gcaataatca tttataccga aaagtgagaa tgtgtgaaat
      601 aaggacaaaa aacctagtaa ctaagggaat ggttcttatt tcgcaccaat cgctccaacc
      661 gcccccgccg cttaggtggt gcgaaataag cgggcaacac ccgataacct aagaga
//
LOCUS       DACBZN010000273          705 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.286, whole genome shotgun sequence.
ACCESSION   DACBZN010000273 DACBZN010000000
VERSION     DACBZN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.286"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..545
                     /locus_tag="IVD64_003033"
     CDS             <1..545
                     /locus_tag="IVD64_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP6150210.1"
                     /translation="PGVQVIRPADGNETRAAWKVAMETTDAPTILVLSRQNLPVLPST
                     KEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLAVKAQKELAEKGKDVSVVSMPS
                     FDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERYVGTEGATITIDHFGASAPGTK
                     ILEEFGFTVENVVNTYNQLS"
BASE COUNT          223 a          121 c          156 g          205 t
ORIGIN      
        1 tgcctggtgt tcaagtgatt cgtccggcag acggaaatga aacacgtgct gcttggaaag
       61 tagcgatgga gacaacagat gcgcctacta tccttgtatt gagtcgtcaa aaccttccag
      121 tattgccttc tacaaaagaa gtagcagatg aaatggtgaa aaaaggcgcg tatgttcttt
      181 ctccatcaca aggagaaaca ccagaaggta ttttgattgc aacaggttct gaagttgatt
      241 tggctgtgaa agctcaaaaa gaattggctg aaaaaggcaa agatgtttct gttgtttcta
      301 tgccaagctt tgacctcttt gaaaaacaat cttctgaata taaagaaagt gtacttccta
      361 aatcagtgaa gaaacgtgtg gctatcgaag ctgctgcatc atttggatgg gaacgctacg
      421 taggaacaga gggcgcaacg attacaatcg atcatttcgg tgcatctgcc cctggcacta
      481 aaattttaga agaatttgga tttacagtag aaaatgtcgt gaatacgtac aatcaactat
      541 catagatcca agcaattgtt tgactgataa gaacagctgt ctgcaagttg gagacagctg
      601 ttctttatgt gttagaagaa taaatcaata ttacttttcg ttttcggcta tgttacattt
      661 aaatgacaaa acaaagaatt cttcggatta gtttgtgttt cttga
//
LOCUS       DACBZN010000274          698 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.287, whole genome shotgun sequence.
ACCESSION   DACBZN010000274 DACBZN010000000
VERSION     DACBZN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.287"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
BASE COUNT          247 a          116 c           85 g          250 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatta atggaatggc aacagttt
//
LOCUS       DACBZN010000275          678 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.288, whole genome shotgun sequence.
ACCESSION   DACBZN010000275 DACBZN010000000
VERSION     DACBZN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.288"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(71..144)
                     /locus_tag="IVD64_003034"
     tRNA            complement(71..144)
                     /locus_tag="IVD64_003034"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Arg,seq:acg)
     gene            complement(155..240)
                     /locus_tag="IVD64_003035"
     tRNA            complement(155..240)
                     /locus_tag="IVD64_003035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Leu,seq:taa)
     gene            complement(257..328)
                     /locus_tag="IVD64_003036"
     tRNA            complement(257..328)
                     /locus_tag="IVD64_003036"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:Gly,seq:gcc)
     gene            complement(340..412)
                     /locus_tag="IVD64_003037"
     tRNA            complement(340..412)
                     /locus_tag="IVD64_003037"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(377..379),aa:Thr,seq:tgt)
     gene            complement(430..511)
                     /locus_tag="IVD64_003038"
     tRNA            complement(430..511)
                     /locus_tag="IVD64_003038"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(475..477),aa:Leu,seq:tag)
     gene            complement(519..591)
                     /locus_tag="IVD64_003039"
     tRNA            complement(519..591)
                     /locus_tag="IVD64_003039"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Lys,seq:ttt)
     gene            complement(595..667)
                     /locus_tag="IVD64_003040"
     tRNA            complement(595..667)
                     /locus_tag="IVD64_003040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(632..634),aa:Val,seq:tac)
BASE COUNT          180 a          195 c          155 g          148 t
ORIGIN      
        1 gaccccttgg tcccaaacca agtgctctac caagctgagc tacttcccgt ttaaaaaata
       61 aaaaagacta cgcgcccaag aggagtcgaa cccctaacct tttgatccgt agtcaaacac
      121 tctatccagt tgagctatgg gcgcatattt gaaatgccga ggaccggaat cgaaccggta
      181 cggtgatcac tcaccgcagg attttaagtc ctgtgcgtct gccagttccg ccaccccggc
      241 gcgattgctt tggcaaagcg gaaaacgggg ttcgaacccg cgacccccac cttggcaagg
      301 tggtgctcta ccactgagct atttccgcgt gtttaaagat gccggctaaa ggacttgaac
      361 cctcgaccct ctgattacaa atcagatgct ctaccaactg agctaagccg gcatgtaaaa
      421 cttaaattat gcgggtgaag ggacttgaac ccccacgccg taaggcgcta gatcctaaat
      481 ctagtgcgtc tgccaattcc gccacacccg caaaaatatg agccgtacag ggctcgaacc
      541 tgtgaccctc tgattaaaag tcagatgctc taccaactga gctaacggct catatggagg
      601 ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc agctgagcta
      661 aacctccatg gataactt
//
LOCUS       DACBZN010000276          670 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.289, whole genome shotgun sequence.
ACCESSION   DACBZN010000276 DACBZN010000000
VERSION     DACBZN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.289"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..>670)
                     /locus_tag="IVD64_003041"
     rRNA            complement(<1..>670)
                     /locus_tag="IVD64_003041"
                     /product="16S ribosomal RNA"
BASE COUNT          134 a          213 c          147 g          176 t
ORIGIN      
        1 gtttcaacct tgcggtcgta ctccccaggc ggagtgctta atgcgttagc tgcagcactg
       61 aagggcggaa accctccaac acttagcact catcgtttac ggcgtggact accagggtat
      121 ctaatcctgt ttgctcccca cgctttcgag cctcagcgtc agttacagac cagagagccg
      181 ccttcgccac tggtgttcct ccatatatct acgcatttca ccgctacaca tggaattcca
      241 ctctcctctt ctgcactcaa gtctcccagt ttccaatgac cctccccggt tgagccgggg
      301 gctttcacat cagacttaag aaaccgcctg cgctcgcttt acgcccaata aatccggaca
      361 acgcttgcca cctacgtatt accgcggctg ctggcacgta gttagccgtg gctttctggt
      421 tagataccgt caagggatga acagttactc tcatccttgt tcttctctaa caacagagtt
      481 ttacgatccg aaaaccttct tcactcacgc ggcgttgctc ggtcagactt tcgtccattg
      541 ccgaagattc cctactgctg cctcccgtag gagtttgggc cgtgtctcag tcccaatgtg
      601 gccgatcacc ctctcaggtc ggctatgcat cgtggccttg gtgagccgtt acctcaccaa
      661 ctagctaatg
//
LOCUS       DACBZN010000277          658 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.290, whole genome shotgun sequence.
ACCESSION   DACBZN010000277 DACBZN010000000
VERSION     DACBZN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.290"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVD64_003042"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVD64_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP6150211.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          144 c          111 g          205 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatc
//
LOCUS       DACBZN010000278          625 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.291, whole genome shotgun sequence.
ACCESSION   DACBZN010000278 DACBZN010000000
VERSION     DACBZN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.291"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            1..625
                     /locus_tag="IVD64_003043"
                     /pseudo
     CDS             1..625
                     /locus_tag="IVD64_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          214 a          110 c          118 g          183 t
ORIGIN      
        1 atgactaaag tgctgactga agaagtaaat gcatttaaag ttagaactct aaatgataag
       61 tatgtcgcta ttttcatgga tgctacttat attcctttaa aacggcaaac cgtctccaaa
      121 gaagcaatct atattgccat tggtatacga gaagacggca ctaaagaagt actaagttat
      181 gcgattgctc cgactgaatc gacctacgtt tggaatgagt tgttacagga catttactcc
      241 agaggggttc atgatgtctt actgtttatt accgatggct taaaaggcat gaaagatacg
      301 attcaccaga tttatcctaa agctaaatac caacattgct gtgttcacgt ttcccgtaat
      361 attgctcata aagtacgtgt caaagatcga aaagaaatct gtgatgattt taaggctgtt
      421 tatcaagcta gctcaaagga agaggcaaat acctttttag ggagtatgat tgagaagtag
      481 caaaaaactt atcctaaagt gacgcagtca ctgataaaaa atcaagattt attgactttt
      541 tatgagtttc cgcctggtat ccgccgaagc atttactcaa ctaatttaat cgaatctttc
      601 aagaaacaaa tcaagaaata cagcc
//
LOCUS       DACBZN010000279          625 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.292, whole genome shotgun sequence.
ACCESSION   DACBZN010000279 DACBZN010000000
VERSION     DACBZN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.292"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>625
                     /locus_tag="IVD64_003044"
     CDS             <1..>625
                     /locus_tag="IVD64_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6150212.1"
                     /translation="LVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQ
                     VMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDD
                     SLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQTEIKRALR
                     VPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          205 a          135 c          124 g          161 t
ORIGIN      
        1 gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag tgttttccga
       61 atgcccgaat cgtcgccgat cgctttcata ttagccaaca cattggtcgg gcttttacca
      121 accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg aagcacttaa
      181 agaaatactg gaaactactt caaaagaatg cgtgggaatt gaaaggtcag catcgctact
      241 ggcgtccgtc atttcgagat catctgacag aagcagagat agtcgatcgg ctattatctt
      301 atgatgattc cttaaagcga ggctacgaag tctaccagga ttttctttcc gttatcagaa
      361 gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag ttgccagagc
      421 actaccaacc agtgttcacg accttcaaga aataccaaac agaaatcaaa cgagcgttgc
      481 gcgttcccta ttccaacggc cctatagaat gtttgaataa ccatatcaaa gtactaaaac
      541 gaattgccta tggctataga aactttcaaa actaccgaga acgaatcttt ttatatcgag
      601 gaaaatattt taagaaaact aagaa
//
LOCUS       DACBZN010000280          623 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.293, whole genome shotgun sequence.
ACCESSION   DACBZN010000280 DACBZN010000000
VERSION     DACBZN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.293"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(105..314)
                     /locus_tag="IVD64_003045"
     CDS             complement(105..314)
                     /locus_tag="IVD64_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150213.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(381..620)
                     /locus_tag="IVD64_003046"
     CDS             complement(381..620)
                     /locus_tag="IVD64_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150214.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT          167 a          136 c           74 g          246 t
ORIGIN      
        1 gacgattaaa gtgactccta aaaaaccaaa taaccatttt ttcatccatc tgcctccttt
       61 cagacaaaac aaaaaaactt tttctcattt ccatagaaaa agttttaaaa atgaattcta
      121 taagttcgtc tcaactgttc aaagtttttc attgaaccta acaaacaagc tttctctttt
      181 cctgttaact gcatatcatt gatatattgc gcgacagata atcttttaag gtgtttttgc
      241 ttcatcgttt ttagtaactt ttcttccatt tctttcgaca ctttttttct gttttcgatt
      301 tcttcataat tcatgagttt tcctaccttc cttgtttttt gcaaaaataa aaaagcctat
      361 gatcacatac gctctacact tcagtcttgg gttatctttt tctttcgttt aattactggc
      421 cgtgctggtt tcaaatcctt gattccatta tgggttctcc aataatcgaa ttcttttcgc
      481 aatctttctt tttccatatc tgttccttta gataaggctt tgaatacttc tttaggtgaa
      541 taaccacgag ctaacagctg tacatacctt ccttttgtca aagtatccat atccatcgcc
      601 tctaaaactg gtcgcgccat cta
//
LOCUS       DACBZN010000281          615 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.294, whole genome shotgun sequence.
ACCESSION   DACBZN010000281 DACBZN010000000
VERSION     DACBZN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.294"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..104
                     /locus_tag="IVD64_003047"
     CDS             <1..104
                     /locus_tag="IVD64_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP6150215.1"
                     /translation="GTVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..>615
                     /locus_tag="IVD64_003048"
     CDS             104..>615
                     /locus_tag="IVD64_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150216.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          208 a           97 c          149 g          161 t
ORIGIN      
        1 ttggaactgt agaattcccg attgaagaag gagccttgat catcgcaacc gttgatgatc
       61 caaagcaaaa tgtgttcact cttgagaaag tcggggtgat ctaatggcta aaactaaacg
      121 aatgatggaa acggatgaaa aaacaggtgt acaacgccaa ttctttccaa tcacacatgc
      181 ttccgcagtt cttgggttag aagaaataat agcaggagaa gcaacagttt tatctgttaa
      241 tggaaaaatt ggagccgtcg tcattactaa agaagatttg ggattagaga atgttctcac
      301 agaattaccc tatgcaagtg aagaagatga cggcattatc acggcagaaa tgtatcaaaa
      361 aattttaaac agtggagaag gtgactacgt gttgccagtt gccactatcg accgtttggg
      421 tggcataaaa gttggtgaat tattgacgat tgatgagaca gggaaagtct ctgcagtcag
      481 acaatctgat gtcaattttt cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta
      541 tactgctgga gaaaacatca ctattgatga agatggaaca attagttcta caggtggtag
      601 tgggactggt ggtgt
//
LOCUS       DACBZN010000282          607 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.295, whole genome shotgun sequence.
ACCESSION   DACBZN010000282 DACBZN010000000
VERSION     DACBZN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.295"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..>607)
                     /locus_tag="IVD64_003049"
     rRNA            complement(<1..>607)
                     /locus_tag="IVD64_003049"
                     /product="23S ribosomal RNA"
BASE COUNT          122 a          187 c          123 g          175 t
ORIGIN      
        1 ctcctcttaa ccttccagca ccgggcaggc gtcagcccct atacttcatc ttacgatttt
       61 gcagagacct gtgtttttga taaacagtcg cttgggccta ttcactgcgg ctgatcgtat
      121 gatcagcacc ccttctcccg aagttacggg gtcattttgc cgagttcctt aacgagagtt
      181 ctctcgctca ccttaggatt ctctcctcga ctacctgtgt cggtttgcgg tacgggccgc
      241 tgttttctca ctagaagctt ttctcggcag tgtgacatca ggaacttcgg tactattatt
      301 tccctcccca tcacagcttg tccttaagaa aagaagcatt tgactcctct tcagacttac
      361 tgcttggaca gacatttccg atcgtctgca ttccttagcc tcctgcgtcc ctccattgct
      421 caaacaaaaa caacgggtac aggaatatca acctgttatc catcgcctac gcctgtcggc
      481 ctcggcttag gtcccgacta accctgggcg gacgagcctt ccccaggaaa ccttagtcat
      541 tcggtggaca ggattctcac ctgtctttcg ctactcatac cggcattctc acttctaagc
      601 gctccag
//
LOCUS       DACBZN010000283          606 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.296, whole genome shotgun sequence.
ACCESSION   DACBZN010000283 DACBZN010000000
VERSION     DACBZN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.296"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>606
                     /locus_tag="IVD64_003050"
     CDS             <1..>606
                     /locus_tag="IVD64_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6150217.1"
                     /translation="ELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSVIDE
                     LNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRSGMI
                     TQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEAGNG
                     SVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILV"
BASE COUNT          232 a           90 c          142 g          142 t
ORIGIN      
        1 agaattattg aaaaaaatga aggcacgtcg tgagcaacga ttgactgaat tacgtgaaaa
       61 agttgaatca ggagaattac gcgaagcaga tttagaagcc gtgaaagaag aaattgacag
      121 tgttattgat gaattgaacg gaattaaaga cgaattaggc gcagattctg gaactgatga
      181 aacagacgac aatacagatg atcaatcgaa tagtacggat tcagacgaaa gccgttctgg
      241 tgaagacaac gatcaagaag aagattcaga cagtgaagat agttcagaaa atcgttctgg
      301 aatgattact caacagcaac gagatggatt acttggatca attaagaacg gattggaggc
      361 acgtgcaaaa atgaccaaga aacaaaaaga tcaacaacta cgaaaagcat ttgctaattt
      421 tgtagctgga aatatttctg aagcagaagc tcgagcttta gggattgaag ctggcaacgg
      481 ttcagttact gtcccagaag taattgcatc tgaagttatt acttatgctc aagaagaaaa
      541 tttacttcgt aaatacggaa cagtggtgcg aacatcagga gatgtcaaat atccaattct
      601 tgtgaa
//
LOCUS       DACBZN010000284          601 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.297, whole genome shotgun sequence.
ACCESSION   DACBZN010000284 DACBZN010000000
VERSION     DACBZN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.297"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..450
                     /locus_tag="IVD64_003051"
     CDS             <1..450
                     /locus_tag="IVD64_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAP6150218.1"
                     /translation="PFPGSSLDKSSFSGGQLFGTNPGGEFRPNGFHDGLDFGSVDHPG
                     SEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKV
                     GQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLPFLNSSKK"
BASE COUNT          190 a           93 c          151 g          167 t
ORIGIN      
        1 ccattccctg gcagcagttt agataaaagt tccttcagcg gtggtcaatt atttggaacg
       61 aatccaggcg gtgaattccg tccgaacggc ttccatgatg gattagattt tggttcagtt
      121 gatcatccag gaagtgagat tcatgcggtt catggcggaa aagtcgtgta cgttggcaat
      181 cctggtattt caggtttggg cgcgtgtgtg atcgtgatta attacgatgg cttgaatatg
      241 gtgtatcaag aatttgcaaa tagtacagga aattctcgag tgaaagtagg cgatcaagtg
      301 aaagttggtc aagtaattgg gatacgagat acagcacact tgcatttagg atttactcga
      361 atggattggc gtcaagcaca aggacatgca ttcattgatg atggtacatg gatcgatccg
      421 ttaccatttt tgaatagcag taaaaaatag atcaggagaa aaaatatgga aaataaaacg
      481 ttaaaaatcg tcttaacagc tgcaattagc gttagtttaa tcggcaatat tgcgttgttt
      541 tggcagctga aagaggaaca agcagcaaat acctataaaa ttgaagaagt ggctaaaaac
      601 g
//
LOCUS       DACBZN010000285          593 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.298, whole genome shotgun sequence.
ACCESSION   DACBZN010000285 DACBZN010000000
VERSION     DACBZN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.298"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(318..581)
                     /locus_tag="IVD64_003052"
     CDS             complement(318..581)
                     /locus_tag="IVD64_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP6150219.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACBZN010000286          591 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.299, whole genome shotgun sequence.
ACCESSION   DACBZN010000286 DACBZN010000000
VERSION     DACBZN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.299"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..523
                     /locus_tag="IVD64_003053"
     CDS             <1..523
                     /locus_tag="IVD64_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6150220.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
BASE COUNT          228 a           90 c          139 g          134 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagcggcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc
      241 cgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt
      361 aaaaggaaaa agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaatcca ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttg aaaatgaccg a
//
LOCUS       DACBZN010000287          588 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.300, whole genome shotgun sequence.
ACCESSION   DACBZN010000287 DACBZN010000000
VERSION     DACBZN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.300"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
BASE COUNT          192 a           83 c           83 g          230 t
ORIGIN      
        1 aaattataga gccatttttt cttgatgtga tatccttgtc ttttttgtaa tgtttttaac
       61 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
      121 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      181 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      241 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      301 ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga gtaattaatt
      361 agatgtttat tttataggac tgatactata gattacaaat agtatcgtat ttcgcaatct
      421 taaaattatc ttcttactgt gaataagata ataaaatttg tatttgtcct ttttaggtaa
      481 cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat agtcataacg
      541 ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatctttt
//
LOCUS       DACBZN010000288          586 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.301, whole genome shotgun sequence.
ACCESSION   DACBZN010000288 DACBZN010000000
VERSION     DACBZN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.301"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            70..333
                     /locus_tag="IVD64_003054"
     CDS             70..333
                     /locus_tag="IVD64_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6150221.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            333..>586
                     /locus_tag="IVD64_003055"
     CDS             333..>586
                     /locus_tag="IVD64_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6150222.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          221 a           72 c          126 g          167 t
ORIGIN      
        1 aaaacaatcg aatttacgta cttaattaag tacgttagaa tggagatata aattagaggg
       61 ggagtagata tggcaaaaac agtcttaaat cctagctcag cacggaaaaa tttttataag
      121 ttgttgaaag atgtaaatga gaatcatacg gaaattgaaa tcattagtga gcgcagtgaa
      181 aataatgcgg tgcttattgg tttggatgat tggaaagcaa tcaaagaaac gctattactt
      241 gagcaaatag gcactttggg tgttgttcgt aagcgagaaa aagatgatag tggatttact
      301 aatgttgatg aaattgattg ggataactta taatgaataa ctattctgtg atgattaaaa
      361 actcggctaa agctgattta aagaaaatca aacagtctaa tttgaaacct caatttgaga
      421 aggttattca gactttaaag gaagatccct atctccctac ccaatcattt gagaagttaa
      481 aacctacaca tgaaggtagg tattctagaa gattgaatag acaacaccgg gttgtttata
      541 aggtagatga agatgaacac gtggttgaaa tttattcagc aaggac
//
LOCUS       DACBZN010000289          578 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.302, whole genome shotgun sequence.
ACCESSION   DACBZN010000289 DACBZN010000000
VERSION     DACBZN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.302"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..413
                     /locus_tag="IVD64_003056"
     CDS             <1..413
                     /locus_tag="IVD64_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150223.1"
                     /translation="LVQTDGDQAIAGHKNFSGSVTINNKRVLTTDDNRYEVVNLVVTN
                     GNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYRTPISFEQLVGSIDRST
                     LNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          200 a           91 c          122 g          165 t
ORIGIN      
        1 cattggtgca gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg
       61 taacaatcaa taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc
      121 tggttgtcac taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt
      181 ctatatattt tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa
      241 ctgttccaga aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc
      301 gttctacttt gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa
      361 gaaattcaag ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga
      421 aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg
      481 aacaatcaag aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg
      541 agaccaagat ttgattttga taaaaaacag tgggttga
//
LOCUS       DACBZN010000290          548 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.303, whole genome shotgun sequence.
ACCESSION   DACBZN010000290 DACBZN010000000
VERSION     DACBZN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.303"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            87..>548
                     /locus_tag="IVD64_003057"
     CDS             87..>548
                     /locus_tag="IVD64_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150224.1"
                     /translation="MKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDN
                     QYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQS
                     VKNEPVSKEQMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHS"
BASE COUNT          223 a           89 c          121 g          115 t
ORIGIN      
        1 aggaatctaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt cgtgaattac
       61 ttgaagaggc tgtaccaact gattttttga aggagaaaga aaacgatcat ttttatcgag
      121 tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg gagatcgtaa
      181 cattcgaatt gatcgatcaa ctcaaaacgt tggataacca atacgccgca gaagcaggtt
      241 attataaatt ctattttgaa gaagtgaaga acggagaagt agtcgatcag atccgaatgg
      301 atctagggga tggcgtcaac agtaatcaac ccatctattt agagttagca aaattgtgcg
      361 aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt tcgaaagaac
      421 aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga acggcagaag
      481 agattaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg aaaaagcata
      541 ctcattct
//
LOCUS       DACBZN010000291          540 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.304, whole genome shotgun sequence.
ACCESSION   DACBZN010000291 DACBZN010000000
VERSION     DACBZN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.304"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>540
                     /locus_tag="IVD64_003058"
     CDS             <1..>540
                     /locus_tag="IVD64_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6150225.1"
                     /translation="ELLKKMKARREQRLTELREKVESGELREADLEAITKEIDAVVDE
                     LNGIKSELSEADGSDEGSDADEGTGGSDGSTGSDENRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYA"
BASE COUNT          201 a           80 c          132 g          127 t
ORIGIN      
        1 agaattattg aaaaaaatga aggcacgtcg tgaacaacga ttgactgaat tacgtgaaaa
       61 agttgaatca ggagaattac gcgaagctga tttagaagct attacaaaag aaattgatgc
      121 cgttgttgat gaattgaacg gaatcaaaag tgaactaagt gaagccgatg gatcagatga
      181 aggttcagat gctgacgaag gaacaggtgg atcagacggc tcgactggaa gcgatgaaaa
      241 tcgttctggt gaagacaacg atcaagaaga agattcagac agtgaagata gttcagaaaa
      301 tcgttctgga atgattactc aacagcaacg agatggatta cttggatcaa ttaagaacgg
      361 attggaggca cgtgcaaaaa tgaccaataa acaaaaagat caacaactac gaaaagcatt
      421 tgctaatttt gtagttggaa atatttctga agcagaagct cgagctttag ggattgaagc
      481 tggcaacggc tcagttactg tcccagaagt aattgcatct gaagttatta cttatgctca
//
LOCUS       DACBZN010000292          525 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.305, whole genome shotgun sequence.
ACCESSION   DACBZN010000292 DACBZN010000000
VERSION     DACBZN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.305"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            185..481
                     /locus_tag="IVD64_003059"
     CDS             185..481
                     /locus_tag="IVD64_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150226.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
BASE COUNT          212 a           65 c           97 g          151 t
ORIGIN      
        1 cccactttta aaaaaaagtg gttggatagg ctgtgcctat cacgtttctg aaaaaaataa
       61 atgaccccaa aataccaata tataggatag tacaaggata gcaaaggata gtaaaaggat
      121 agaggtagga tagcgaaaag gataggatcc ttttcactat cctatattat attggaggat
      181 atttatgaat gttaaaatta gagatttaga tccaaaattc atttctgaaa ttgatcgaag
      241 atgcgttgaa ctaagtaatc gtacaggaaa taaatggagt cgtaatgatt atttaaagct
      301 actcgttgaa aatgattttg atcgtccttt gatggaatac aaacaagaaa agtttgacca
      361 gttattagaa aagttttcag aaattcaaag ttataacaca aagatattag aagaatatgt
      421 tagccaaaat aatcgtatta tagaaatctt gattgagcaa aatagaggaa gtgaactttg
      481 atatggcaaa ggttatagac aaacacattt actttcacca aacac
//
LOCUS       DACBZN010000293          514 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.306, whole genome shotgun sequence.
ACCESSION   DACBZN010000293 DACBZN010000000
VERSION     DACBZN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.306"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..132)
                     /locus_tag="IVD64_003060"
     CDS             complement(<1..132)
                     /locus_tag="IVD64_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6150227.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACBZN010000294          497 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.307, whole genome shotgun sequence.
ACCESSION   DACBZN010000294 DACBZN010000000
VERSION     DACBZN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.307"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            110..334
                     /locus_tag="IVD64_003061"
     CDS             110..334
                     /locus_tag="IVD64_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP6150228.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          221 a           62 c           97 g          117 t
ORIGIN      
        1 attttccgtg acggctcttt ttactatatt aaaatcatag aaatgtctaa tcaaaaaaat
       61 ataaaaatta ttttgcgtac atatgattaa agaaaggaga gtgtgacaca tggaaacaga
      121 atggcaaaga cgtgaaagaa aaaaacagaa aaaagaagag atgttacgat tatgtcgatt
      181 attagatgta aaagactggg acaaaaacga gcaaattgca caaaaagtac gatcaatcat
      241 ccataaagag gaaaaagtta aaaataaaga cacgcgtaga actgaaaatg aaaagagaaa
      301 agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa aaagaggctg agacagaagc
      361 gtttaactcc aagaaataag ctggaattca caaaaatttg aaaagcaatt ttcgtgaatt
      421 tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt tatacgtgtt tagagcgtga
      481 gacaaaagtg ttcttta
//
LOCUS       DACBZN010000295          496 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.308, whole genome shotgun sequence.
ACCESSION   DACBZN010000295 DACBZN010000000
VERSION     DACBZN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.308"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            1..270
                     /locus_tag="IVD64_003062"
                     /pseudo
     CDS             1..270
                     /locus_tag="IVD64_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          186 a           73 c           87 g          150 t
ORIGIN      
        1 aaatctatat tttgatatac aaattacagc ttcagatact aagttgcata aaccttttcc
       61 ggaccccata caaaaggcaa ttgatgaatt gggcataaaa aataagaaac gatttatatt
      121 ggtgattcat tatatgacat gcaaagtgcg aaagcttgtg ggatagcatt tggtttagct
      181 aaatggggag ccaaaaatat agaaaatttt caaagtgtag atataattat tgattctcct
      241 caagaaatac ttgatttgtt agaaaaatag acaaagagcg tgagataagg atagggtctc
      301 acgctctttg tttttcgcca tttaaagatt gaaatttttg ccatcgccga ttgtaaaatt
      361 aagctagaca actaaaaaag tcatttgtgc tacactcaaa atagttccga ccaaagaatt
      421 ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt gtaataatag
      481 agtcttattt caaaat
//
LOCUS       DACBZN010000296          482 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.309, whole genome shotgun sequence.
ACCESSION   DACBZN010000296 DACBZN010000000
VERSION     DACBZN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.309"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..408)
                     /locus_tag="IVD64_003063"
     CDS             complement(<1..408)
                     /locus_tag="IVD64_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150229.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKP"
BASE COUNT          179 a           78 c           62 g          163 t
ORIGIN      
        1 cggctttcca aatgatgaaa tacctaaatc taacttatta agaaagttga aatttttata
       61 ttcaggaaac catttattaa tttcatgtgc agcacgtaat gcattccctg gagttgtcaa
      121 agagaaaatt aattctgcta agcttattat tgaaaatact gataacttat aattatatcg
      181 tccattgaca atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac
      241 ttgttcttga ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt
      301 tatttcttct cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac
      361 tggccactga tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg
      421 tattaaacag tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac
      481 at
//
LOCUS       DACBZN010000297          473 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.310, whole genome shotgun sequence.
ACCESSION   DACBZN010000297 DACBZN010000000
VERSION     DACBZN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.310"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>473
                     /locus_tag="IVD64_003064"
     CDS             <1..>473
                     /locus_tag="IVD64_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6150230.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DACBZN010000298          468 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.311, whole genome shotgun sequence.
ACCESSION   DACBZN010000298 DACBZN010000000
VERSION     DACBZN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.311"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>468
                     /locus_tag="IVD64_003065"
     CDS             <1..>468
                     /locus_tag="IVD64_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6150231.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACBZN010000299          456 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.312, whole genome shotgun sequence.
ACCESSION   DACBZN010000299 DACBZN010000000
VERSION     DACBZN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.312"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..397
                     /locus_tag="IVD64_003066"
     CDS             <1..397
                     /locus_tag="IVD64_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150232.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          161 a           78 c          106 g          111 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt
      361 agcgaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt tcaattaaaa
      421 gaagacggag ttgctaaata cttaaaaacg catgta
//
LOCUS       DACBZN010000300          445 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.313, whole genome shotgun sequence.
ACCESSION   DACBZN010000300 DACBZN010000000
VERSION     DACBZN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.313"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
BASE COUNT          133 a           96 c           60 g          156 t
ORIGIN      
        1 tgcatcaacg aacgtcttcg tagtcagatt taacgttaaa atattgccac tttgataagc
       61 ttttgccgct ttgtctgtga cgacaaaagc tctgcccgtt tgaatttctg tcacaaaaac
      121 gagattatct ctgctttttt caacaacaac tccaatcgtt gaatgaacca tttcttcttc
      181 ctcccttctc tttcttttga tataatgtag tcgcaaatcg caccactcaa tgaattggcg
      241 gaaaacaatt cattgaaaaa aagaatcctg gacaatcacg ttcaggactc tttttttcac
      301 ttaataacta acactctgtg agtgaaatag actcttttat gcttttacat ggaaagttat
      361 tgattccctc ttcattcatt tcccaagtag tataataagg aactaaattc ccttcatatt
      421 cttgaatgat aaaatatttt tcacc
//
LOCUS       DACBZN010000301          443 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.314, whole genome shotgun sequence.
ACCESSION   DACBZN010000301 DACBZN010000000
VERSION     DACBZN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.314"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>443
                     /locus_tag="IVD64_003067"
     CDS             <1..>443
                     /locus_tag="IVD64_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150233.1"
                     /translation="GTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADD
                     TEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKV
                     DVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          179 a           67 c          103 g           94 t
ORIGIN      
        1 ggaacaacag tctttgcagt gaaagacggc gtaagcttac cagaagataa acttccagtg
       61 ttgaaagcaa aagatggtta tacagatgcg aaatggccag aggaagcaac tcaaccaatt
      121 acagcagatg atacagaatt tgtatcaagt gcaacaaaac tggatgataa atctgacgct
      181 gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga accagacgca
      241 tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac ttggaaagaa
      301 aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac atatccagat
      361 ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc tgacgccgat
      421 aaatatgagc caacagtaga agg
//
LOCUS       DACBZN010000302          430 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.315, whole genome shotgun sequence.
ACCESSION   DACBZN010000302 DACBZN010000000
VERSION     DACBZN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.315"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>430
                     /locus_tag="IVD64_003068"
     CDS             <1..>430
                     /locus_tag="IVD64_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP6150234.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DACBZN010000303          420 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.316, whole genome shotgun sequence.
ACCESSION   DACBZN010000303 DACBZN010000000
VERSION     DACBZN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.316"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            74..403
                     /locus_tag="IVD64_003069"
     CDS             74..403
                     /locus_tag="IVD64_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6150235.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DACBZN010000304          418 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.317, whole genome shotgun sequence.
ACCESSION   DACBZN010000304 DACBZN010000000
VERSION     DACBZN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.317"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>418
                     /locus_tag="IVD64_003070"
     CDS             <1..>418
                     /locus_tag="IVD64_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150236.1"
                     /translation="NSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQPMNDKRNGH
                     SWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDMTIENIPRIK
                     EEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEK"
BASE COUNT          190 a           60 c           94 g           74 t
ORIGIN      
        1 aatagtcgtt tgggaaaatc caaaagaaaa ttagtacgat tgcaaaatga attaaacgaa
       61 caaattgaaa agatgtttga acaccagaga aagaccaacg gtcaaccaat gaacgataag
      121 agaaatgggc attcttggtt taggcaacaa gagcgaatcg aaaataaagt gcatagtctg
      181 agagaagaga tcaagcaaca agaaaaacaa gtagaaaaac ttgaaagaca agaagagata
      241 aaggaaatgg gctataacaa gtacggcgga ctggatatga cgatcgagaa tattccaaga
      301 atcaaagaag aaatcgaacg ctttgaaaaa ggtgaatcga ctttttcagc cgctacgatc
      361 cgaaaatacc aacggaaatt ggaaacatta gaacagctga aagaaagatc ggaaaaag
//
LOCUS       DACBZN010000305          401 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.318, whole genome shotgun sequence.
ACCESSION   DACBZN010000305 DACBZN010000000
VERSION     DACBZN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.318"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>401
                     /locus_tag="IVD64_003071"
     CDS             <1..>401
                     /locus_tag="IVD64_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6150237.1"
                     /translation="LLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINY
                     MFNGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRF
                     IINRAALTAIEKMKTDDGFPLLRPFTQAEGG"
BASE COUNT          142 a           58 c           85 g          116 t
ORIGIN      
        1 ttgcttaatc ctgccgaatt cgatgctttg gcaacagtaa ctaaaaaatt attaaaaatg
       61 tctggtgttc cagttgaaga tattgttgtg gaagaattga aaaaagctta tgtgcgtaaa
      121 gaaatcaatt atatgttcaa tggtgatgac gctggaaatg aaaatcctgg tgcattagcc
      181 aaaaaggctg tagcatttaa aaaaccttta gatctaactg cttcaggtgc tgggcaaaaa
      241 ttatatgatg cattaatcga atttaaaaat acaccagtga cagaagtgat gaaaaaggga
      301 cgctttatta ttaatcgagc tgctttgact gccattgaaa aaatgaaaac agatgatgga
      361 tttcctttgt tgcggccatt cacacaagca gaaggtggaa t
//
LOCUS       DACBZN010000306          399 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.319, whole genome shotgun sequence.
ACCESSION   DACBZN010000306 DACBZN010000000
VERSION     DACBZN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.319"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..379
                     /locus_tag="IVD64_003072"
     CDS             <1..379
                     /locus_tag="IVD64_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP6150238.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          138 a           78 c           81 g          102 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaaggga
//
LOCUS       DACBZN010000307          399 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.320, whole genome shotgun sequence.
ACCESSION   DACBZN010000307 DACBZN010000000
VERSION     DACBZN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.320"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
BASE COUNT          148 a           50 c           49 g          152 t
ORIGIN      
        1 ttaacgcaaa acttattaaa aaaagacaat tctagtatac tgaaaataga attaaaaaag
       61 gagaacaaca aatctatgaa aataagtaaa aaagcatttc ttttagtagt tcttatctta
      121 cttagtactt tatattcagt aaattttatg agaaatgctc aagaaattta tacaacaggt
      181 gatttatctt ttcatctttc acgaatcaaa gggttatctt ctatttttga agggccaatc
      241 aactatacaa catttaacaa ttatggagat ggactgaact atttttatcc ttttcttaca
      301 ataattcctg cagtcgtttt ttatggaatt tcaaataact taatattaag ttatgttcta
      361 tatatttggt tattaaatat ttgtacgatt ctgattagt
//
LOCUS       DACBZN010000308          394 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.321, whole genome shotgun sequence.
ACCESSION   DACBZN010000308 DACBZN010000000
VERSION     DACBZN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.321"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..285
                     /locus_tag="IVD64_003073"
     CDS             <1..285
                     /locus_tag="IVD64_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6150239.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DACBZN010000309          391 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.322, whole genome shotgun sequence.
ACCESSION   DACBZN010000309 DACBZN010000000
VERSION     DACBZN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.322"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..>391)
                     /locus_tag="IVD64_003074"
     CDS             complement(<1..>391)
                     /locus_tag="IVD64_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6150240.1"
                     /translation="YWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELR
                     TAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSH
                     SNGVTEGLNNKIKLIKRIAFGYRNFYNF"
BASE COUNT          115 a           68 c           83 g          125 t
ORIGIN      
        1 gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat
       61 tcagcccctc agtgacgccg tttgagtggg acaccttaaa ggcatttgaa atacccgatt
      121 ggtagcgatg gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg
      181 ttttaagggt ttcttgaaag ccttccgtac ctcgtttaac gaaataatag cgtaataatt
      241 gacaaaaatg atacgcagtt cgcaattctt cgttgtagct taacagctca tcaacgattt
      301 ctgtttgact aattggtcgt ttaaacagtc gatgataatg ccgtttgagt ggctccaatg
      361 tcgtggaatc tttgagtaag agtttccaat a
//
LOCUS       DACBZN010000310          384 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.323, whole genome shotgun sequence.
ACCESSION   DACBZN010000310 DACBZN010000000
VERSION     DACBZN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.323"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>384
                     /locus_tag="IVD64_003075"
     CDS             <1..>384
                     /locus_tag="IVD64_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6150241.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIVC"
BASE COUNT          141 a           69 c           76 g           98 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tgca
//
LOCUS       DACBZN010000311          381 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.324, whole genome shotgun sequence.
ACCESSION   DACBZN010000311 DACBZN010000000
VERSION     DACBZN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.324"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(21..94)
                     /locus_tag="IVD64_003076"
     tRNA            complement(21..94)
                     /locus_tag="IVD64_003076"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IVD64_003077"
     tRNA            complement(108..181)
                     /locus_tag="IVD64_003077"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IVD64_003078"
     tRNA            complement(209..281)
                     /locus_tag="IVD64_003078"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IVD64_003079"
     tRNA            complement(301..374)
                     /locus_tag="IVD64_003079"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACBZN010000312          360 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.325, whole genome shotgun sequence.
ACCESSION   DACBZN010000312 DACBZN010000000
VERSION     DACBZN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.325"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..233
                     /locus_tag="IVD64_003080"
     CDS             <1..233
                     /locus_tag="IVD64_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6150242.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT          135 a           63 c           51 g          111 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc gtattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
//
LOCUS       DACBZN010000313          350 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.326, whole genome shotgun sequence.
ACCESSION   DACBZN010000313 DACBZN010000000
VERSION     DACBZN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.326"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..>350)
                     /locus_tag="IVD64_003081"
     CDS             complement(<1..>350)
                     /locus_tag="IVD64_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6150243.1"
                     /translation="CTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIE
                     RIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRN
                     EVDKKLFQARVTSL"
BASE COUNT           86 a           82 c           56 g          126 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttatggtaca
//
LOCUS       DACBZN010000314          337 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.327, whole genome shotgun sequence.
ACCESSION   DACBZN010000314 DACBZN010000000
VERSION     DACBZN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.327"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            122..328
                     /locus_tag="IVD64_003082"
     CDS             122..328
                     /locus_tag="IVD64_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6150244.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           82 g           92 t
ORIGIN      
        1 gataaaattg aacaactgga cgggcaaatt gatgccttgc tttcagaaag agaaatattg
       61 atagaagaaa acaagcgatt gaaaaagaaa gtagcccaac caatgaaggg aggtggatag
      121 tatgaacaat agaacctttg atattttgaa atggatcgct atagtagtta tccccgcttt
      181 agcgactttt gtcggtcttg tcggaaaagg tgttaactgg cggtacacag atataacggt
      241 gatgattatt acaggttttg gagcatttct agggagtatt ttaggtgtat caaatcgaac
      301 gtataagaaa atttcatcag ataaatagga ggataga
//
LOCUS       DACBZN010000315          312 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.328, whole genome shotgun sequence.
ACCESSION   DACBZN010000315 DACBZN010000000
VERSION     DACBZN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.328"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            22..>312
                     /locus_tag="IVD64_003083"
     CDS             22..>312
                     /locus_tag="IVD64_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6150245.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRA"
BASE COUNT          114 a           59 c           62 g           77 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg ca
//
LOCUS       DACBZN010000316          311 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.329, whole genome shotgun sequence.
ACCESSION   DACBZN010000316 DACBZN010000000
VERSION     DACBZN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.329"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(40..>311)
                     /locus_tag="IVD64_003084"
     rRNA            complement(40..>311)
                     /locus_tag="IVD64_003084"
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           83 c           77 g           74 t
ORIGIN      
        1 ctgaacaaag taaaaacaaa ttgtgtagtc tccgtaatat tccttagaaa ggaggtgatc
       61 cagccgcacc ttccgatacg gctaccttgt tacgacttca ccccaatcat ctatcccacc
      121 ttaggcggct ggctccaaaa ggttacctca ccgacttcgg gtgttacaaa ctctcgtggt
      181 gtgacgggcg gtgtgtacaa ggcccgggaa cgtattcacc gcggcgtgct gatccgcgat
      241 tactagcgat tccggcttca tgcaggcgag ttgcagcctg caatccgaac tgagagaagc
      301 tttaagagat t
//
LOCUS       DACBZN010000317          298 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.330, whole genome shotgun sequence.
ACCESSION   DACBZN010000317 DACBZN010000000
VERSION     DACBZN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.330"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
BASE COUNT          127 a           37 c           46 g           88 t
ORIGIN      
        1 aaagtattga ttaaagccta tctcaaagtt gtaaaagcta aagaaatatg tcgagaagtg
       61 aatttatcta acacaacaat atctgagttg aagaatggtc gaaagaattt agatacttta
      121 aaattcagat catttgaagc tctatatgta gctgctaaaa aatattttac agctgataac
      181 aaataaaaaa caggagtatt ttatgtgaaa aaagtatata aaaaatgctt atgaatcagc
      241 tatatcacct taaccatttg ttaatgagat gggagaaaac attaaccacg attctaac
//
LOCUS       DACBZN010000318          291 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.331, whole genome shotgun sequence.
ACCESSION   DACBZN010000318 DACBZN010000000
VERSION     DACBZN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.331"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            1..291
                     /locus_tag="IVD64_003085"
                     /pseudo
     CDS             1..291
                     /locus_tag="IVD64_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          115 a           58 c           55 g           63 t
ORIGIN      
        1 aagaattcaa acaacaaatc gtcgatctct atctcgctgg taagcctcgc gcagaaatta
       61 ttcgagagta tgagcttacg ccttcttctt tcgataaatg gatgaagcaa gcacaatcaa
      121 cgggctcatt caaagaaaga gacaacttaa caccagaaca agcagaattg atcgcactaa
      181 gaaagaaaaa taagcaactc gaaatggaga atgatatttt aaagcaagcg gcgctgatat
      241 tcggacgaaa agacaagtaa ttgatgccaa caagcataaa tattccatat c
//
LOCUS       DACBZN010000319          280 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.332, whole genome shotgun sequence.
ACCESSION   DACBZN010000319 DACBZN010000000
VERSION     DACBZN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.332"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
BASE COUNT           79 a           59 c           53 g           89 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa
//
LOCUS       DACBZN010000320          277 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.333, whole genome shotgun sequence.
ACCESSION   DACBZN010000320 DACBZN010000000
VERSION     DACBZN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.333"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
BASE COUNT          128 a           34 c           42 g           73 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatga
//
LOCUS       DACBZN010000321          274 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.334, whole genome shotgun sequence.
ACCESSION   DACBZN010000321 DACBZN010000000
VERSION     DACBZN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.334"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>274
                     /locus_tag="IVD64_003086"
     CDS             <1..>274
                     /locus_tag="IVD64_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6150246.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMA"
BASE COUNT           66 a           56 c           78 g           74 t
ORIGIN      
        1 gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg
       61 cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga
      121 tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc
      181 atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct
      241 gtccatttca tggcaactgt ttagaaggga tggc
//
LOCUS       DACBZN010000322          251 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.335, whole genome shotgun sequence.
ACCESSION   DACBZN010000322 DACBZN010000000
VERSION     DACBZN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.335"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>251
                     /locus_tag="IVD64_003087"
     CDS             <1..>251
                     /locus_tag="IVD64_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6150247.1"
                     /translation="RLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQ
                     NTELNEYVATLEEERHLSGRKQLADLQVENKKLKEENRS"
BASE COUNT           93 a           33 c           56 g           69 t
ORIGIN      
        1 aggcttcctg atggttcgta tcatttatct gggaatgatt ttgttgattt gtatcaacga
       61 gctacgagag caacaaatgc caagagaaat gctgataaca tgagtgagca atatttacag
      121 atgcttgagc aaaatacaga attaaatgag tatgtagcta cattggaaga agagcgtcat
      181 ttgtctggta gaaaacaatt agcagattta caagtggaaa ataaaaagct gaaagaagaa
      241 aatcgctctt t
//
LOCUS       DACBZN010000323          249 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.336, whole genome shotgun sequence.
ACCESSION   DACBZN010000323 DACBZN010000000
VERSION     DACBZN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.336"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..184
                     /locus_tag="IVD64_003088"
     CDS             <1..184
                     /locus_tag="IVD64_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6150248.1"
                     /translation="QHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKR
                     KNEIYYEQQLSQKERV"
BASE COUNT          104 a           34 c           46 g           65 t
ORIGIN      
        1 acagcataat caagtaattt taaaaggaaa aagttatgga aaagagatcc aaacggcgtt
       61 accttcagaa gaatttgcgc taggaaattc attccgattt tcacttgaga cagtgtcaga
      121 agtggcaaaa cgaaaaaatg aaatctatta tgaacaacaa ttgtcacaaa aagaacgtgt
      181 ataaaacaag taaaaaagac tatctgaaaa acggatagtc ttttttcttt tgggaggttt
      241 taaaatgac
//
LOCUS       DACBZN010000324          248 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.337, whole genome shotgun sequence.
ACCESSION   DACBZN010000324 DACBZN010000000
VERSION     DACBZN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.337"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
BASE COUNT           76 a           46 c           51 g           75 t
ORIGIN      
        1 tatgcttgtg atgaattgat tttcgttctg cacttttgga cgataaattt ggacaattga
       61 aaatacaaaa aaaagaaagg cccctgtaag actcgggaga ctggtaatct ccgtcagtgc
      121 cctacaggaa agcattaagc tcactcctta atgcgtgccc ttcagtgtta cggtattacc
      181 cgcaacggat ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa
      241 ggttaaaa
//
LOCUS       DACBZN010000325          239 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.338, whole genome shotgun sequence.
ACCESSION   DACBZN010000325 DACBZN010000000
VERSION     DACBZN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.338"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>239
                     /locus_tag="IVD64_003089"
     CDS             <1..>239
                     /locus_tag="IVD64_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP6150249.1"
                     /translation="RTKHNVAVFLSAYLSARCRVGQPREPKVTASYSLVPPSTADHIF
                     EAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKP"
BASE COUNT           77 a           49 c           49 g           64 t
ORIGIN      
        1 cgcacaaaac ataatgtcgc tgtgtttctt tcagcatatc tatcagctag atgtagagta
       61 gggcaacctc gagaaccaaa agtgacagca tcttattcct tggttccacc ttctacagct
      121 gatcatatat ttgaagcaga aagaatgctg atcgataaag aagaagcaca ggaagaattt
      181 gagtatctgc ataaattgtt tgttcgaggc tattcagcga tacagcatcc gcataagcc
//
LOCUS       DACBZN010000326          239 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.339, whole genome shotgun sequence.
ACCESSION   DACBZN010000326 DACBZN010000000
VERSION     DACBZN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.339"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..>239)
                     /locus_tag="IVD64_003090"
     CDS             complement(<1..>239)
                     /locus_tag="IVD64_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP6150250.1"
                     /translation="RTKHNVGVFLSAYLSARCRVGQPREPKVTASYSLVPPSTANHDF
                     EAERMLIDKEEAQEEFEYLHKLFIRGYSAIQHPHKP"
BASE COUNT           65 a           45 c           47 g           82 t
ORIGIN      
        1 ggtttgtgcg gatgctgtat agcagagtat cctctaataa acaatttatg caaatactca
       61 aattcttctt gtgcttcttc tttatcaatc aacattcttt cggcttcaaa atcatgatta
      121 gctgtagatg gtggaaccaa ggagtatgat gctgtcactt ttggttctcg aggttgccct
      181 actctacatc tagctgatag atatgctgaa aggaaaacac caacattatg ttttgtgcg
//
LOCUS       DACBZN010000327          239 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.340, whole genome shotgun sequence.
ACCESSION   DACBZN010000327 DACBZN010000000
VERSION     DACBZN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.340"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>239
                     /locus_tag="IVD64_003091"
     rRNA            <1..>239
                     /locus_tag="IVD64_003091"
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           70 g           58 t
ORIGIN      
        1 gtttaattcg aagcaacgcg aagaacctta ccaggtcttg acatcctttg accactctag
       61 agatagagct tccccttcgg gggcaaagtg acaggtggtg catggttgtc gtcagctcgt
      121 gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc cttattgtta gttgccatca
      181 ttcagttggg cactctagca agactgccgg tgacaaaccg gaggaaggtg gggatgacg
//
LOCUS       DACBZN010000328          239 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.341, whole genome shotgun sequence.
ACCESSION   DACBZN010000328 DACBZN010000000
VERSION     DACBZN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.341"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            16..222
                     /locus_tag="IVD64_003092"
     CDS             16..222
                     /locus_tag="IVD64_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6150251.1"
                     /translation="MNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRVAYEII
                     ENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT           84 a           24 c           51 g           80 t
ORIGIN      
        1 ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc atataagggt
       61 gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata tgcgggcaca
      121 acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt gttatgtgga
      181 acgcatgaaa acttttatga acagttgaaa aaattaatct agtgatttgt tgtcgttct
//
LOCUS       DACBZN010000329          231 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.342, whole genome shotgun sequence.
ACCESSION   DACBZN010000329 DACBZN010000000
VERSION     DACBZN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.342"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(64..207)
                     /locus_tag="IVD64_003093"
                     /pseudo
     CDS             complement(64..207)
                     /locus_tag="IVD64_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT           82 a           31 c           32 g           86 t
ORIGIN      
        1 ataaaaaaac aaacaaagta aaataatcta ctttgtttgt ttttatttta attaatattg
       61 aatttataaa attgtcttat cgcttggttc cacaaccaag tagcgataag gctcatcccc
      121 ttcagaatga gtggtaacat aaggattttt acttaaaaca gaatgaattt gttttctttc
      181 aaaagcgggc attggttagt caaagactag ttcatttata tttatatctt a
//
LOCUS       DACBZN010000330          230 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.343, whole genome shotgun sequence.
ACCESSION   DACBZN010000330 DACBZN010000000
VERSION     DACBZN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.343"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..>230
                     /locus_tag="IVD64_003094"
     CDS             <1..>230
                     /locus_tag="IVD64_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall surface anchor protein"
                     /protein_id="HAP6150252.1"
                     /translation="AAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKV
                     DKDSYKEENRITIKNNQKASVPMTGSNGFQSYV"
BASE COUNT           99 a           43 c           37 g           51 t
ORIGIN      
        1 gcagcaatcc taaaatcaga taaagagggt cattttgaaa tcactggttt aactgaagga
       61 gattactcac tggaagaaac aaaagctcct gaaaactatc aaaaattaac aaaagagatc
      121 tctttcaaag tagacaaaga ttcatacaaa gaagaaaacc gaatcacgat caaaaataat
      181 caaaaagctt ccgtaccaat gactggtagc aacggtttcc aatcttacgt
//
LOCUS       DACBZN010000331          228 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.denovo.344, whole genome shotgun sequence.
ACCESSION   DACBZN010000331 DACBZN010000000
VERSION     DACBZN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.denovo.344"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            complement(<1..205)
                     /locus_tag="IVD64_003095"
     CDS             complement(<1..205)
                     /locus_tag="IVD64_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6150253.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKK"
BASE COUNT           66 a           47 c           32 g           83 t
ORIGIN      
        1 attttttatt tttctttttc caaatttgat agagtagttt gccatattct tgcacccaac
       61 gataaatcgt cgtatgagaa acgttaatgc cacgatcata taagatttct tgaacttcac
      121 gatagctaag gttataacga agatagtagc ccacggctac aataatcaca tcctgctgaa
      181 attgctttcc tttaaaatga ttcatcgtca ttcctcctgc tatctttt
//
LOCUS       DACBZN010000332         6299 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.126, whole genome shotgun sequence.
ACCESSION   DACBZN010000332 DACBZN010000000
VERSION     DACBZN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.126"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            345..1559
                     /locus_tag="IVD64_003096"
     CDS             345..1559
                     /locus_tag="IVD64_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6150254.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1590..1808
                     /locus_tag="IVD64_003097"
                     /pseudo
     CDS             1590..1808
                     /locus_tag="IVD64_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2227..2700
                     /gene="dfrF"
                     /locus_tag="IVD64_003098"
     CDS             2227..2700
                     /gene="dfrF"
                     /locus_tag="IVD64_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP6150255.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2827..3483
                     /locus_tag="IVD64_003099"
     CDS             2827..3483
                     /locus_tag="IVD64_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP6150256.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3665..4195
                     /locus_tag="IVD64_003100"
     CDS             3665..4195
                     /locus_tag="IVD64_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6150257.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            4741..4983
                     /locus_tag="IVD64_003101"
     CDS             4741..4983
                     /locus_tag="IVD64_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP6150258.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            4980..5450
                     /locus_tag="IVD64_003102"
     CDS             4980..5450
                     /locus_tag="IVD64_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6150259.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            5578..>6299
                     /locus_tag="IVD64_003103"
     CDS             5578..>6299
                     /locus_tag="IVD64_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6150260.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYE"
BASE COUNT         2144 a          906 c         1311 g         1938 t
ORIGIN      
        1 aatcaattct gattcatcag tcccataaat tatagagccc aaatttgaga accgtagcga
       61 cactcgctgc ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa
      121 aatatcttta aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt
      181 tattttttgt ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt
      241 aattgtctgt cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact
      301 acaccttctt ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta
      361 tcaatacagg agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc
      421 ctggtctagc ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt
      481 ctcaatcttt tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat
      541 tggcctttgg acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga
      601 gacatgttac aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct
      661 ttctttacca actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag
      721 ggagattgaa tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt
      781 tccctgtttg ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt
      841 tcgctggtgg agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca
      901 cacttggtca aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag
      961 ctgctatcgg tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac
     1021 ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg
     1081 cgatgattac ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg
     1141 tactaacagg atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag
     1201 caaaaagtgc catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt
     1261 ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg
     1321 gattcacagg tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc
     1381 caagcagctg gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg
     1441 cctatgtgct gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta
     1501 caaaagaaga acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa
     1561 acaaattcaa atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa
     1621 aaaaattaac gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg
     1681 aatatcccac attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt
     1741 tgctatggtt tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg
     1801 aaaaaggtgt ttcccaaaca aaaagatatt aagaaacagc ccatgtttac ctgcttgcaa
     1861 acaaaagaat attgctacta gactaagaga tactacataa gtgtgttggt atctcttttt
     1921 tgttgccgtg aaacagcaga ctatttcatt tctgcttgat taccttaaaa cttattagtc
     1981 tatggtttgt caagggcttg ttgcgtgaga aatgctgaat atcacccttt acaaaacata
     2041 ggctgataag taacttttca aaagcagaaa agaaatggca atgcttataa caatatgata
     2101 taatgcagat gaagacaaaa aaggaatttg tcgagctggt tagttcgaga taattttgag
     2161 tttgttaaga tgtggtgcac ttgatggaat cacaggtacg acatcatatt atggaggatg
     2221 aaatagatga taggtttgat tgttgcgagg tcaaagaata atgttatagg caagaatggt
     2281 aatataccat ggaaaataaa gggagaacaa aagcaattta gagagttaac aacgggtaat
     2341 gtggttatta tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc taatagaatg
     2401 aatattgttg tttccaccac aacagagtat caaggagata atttagtttc agttaaatca
     2461 ttagaagatg cattattatt ggctaaagga cgagatgtat acatatctgg tggatatgga
     2521 ctatttaagg aagctttgca aatagtagat aaaatgtata tcacagaagt agatttaaat
     2581 attgaagatg gagatacatt ctttccagaa tttgatatca atgattttga agttttgata
     2641 ggggaaacac ttggtgagga agtgaaatat acgagaacat tttatgtaag gaaaaaatga
     2701 attgagtaaa ttttggattt agggtgtttt cataaatata ttgcttttta tgcatatata
     2761 aattgttgta agacaaattc cagtttgtat ggggcgataa aatcagaatt tgagagagga
     2821 ttgaatatga ataaggaatg gtctgaactt aataaaacaa tgcaggcaca aataaaaaag
     2881 aaagatactt ataagagggg tattgatact ttacttactt tacgaagtca gttaatacaa
     2941 accttagtat ctttcaaaga agaattatgc agagaagatt ttaactcaat ccccttcata
     3001 aacgctgatg gttatcatag taagacgatt gcttattcta tttggcatat tttccgcatt
     3061 gaagatatag ttgtgcatac agtgataaat gaagatgaac aagtattttt tgcaggcaat
     3121 tatcaagagc gtatcaattc acctatcatt acaacaggaa atgaactcat gaaacagcag
     3181 attgcagact tttcaaaaca gcttaatctg gaagaattat atttatatat tttcgaagta
     3241 tgggaaagca ctgagaaaat gcttgaacgg ttatcttatg atgaattgaa aagaaaaata
     3301 ccgaaagaaa gaaaaggata cttagaatcg ttgaatgtag taaacgacaa tgaaaaagca
     3361 atttggctga ttgattattg gtgtaataaa gatatttgtg gactaattca aatgccgttt
     3421 tcaagacatt ggattatgca cacagaagcc tgtttgcgta taaagaacaa gatacattca
     3481 taggcaaaaa tttcagtttg tagaactaag gaaattggaa tttaaggagg aataaagtat
     3541 ggttagtgat tttataagag cagcatttcc atggatttta atgggtttat ttgtagcaat
     3601 cagttgttcc cttatgagta aaaaagagaa ataaattcct gtttgtattt ataaaagaga
     3661 aaaaatgaga aaagtagttt tatttattgc catgagcctt gacggatata ttgcggacgg
     3721 taatggtgga gtagcttggc taaatggtca tggaaatgac aatgaaaaca tcgacactta
     3781 tacagaattt accaaagata tagatactgt cttaatgggg tggaatactt ctcatcaggt
     3841 tgttactgaa ctttctccgc aagaatgggt atataataaa tttacaactt atgtattaac
     3901 acataaagag tgcaattctt cccaacaaat tcattttaca agtgaaaatc ctgttttatt
     3961 attggaacgg ctaaaacaag aagcaggaaa ggatatttgg atttgtggcg gagcaaatct
     4021 tgttcagcag ttggtaagca aaaatatgat tgataaatat tacatttctg ttattcctac
     4081 tctgttagga aatggggttc gtcttttggg caacatagac agggaaataa agttaaggct
     4141 ttgtaaaaca cagacttata acggaataac cgatttgatt tatatgcgta gataactaga
     4201 gagaaacaga aaagctgtta aaccgacgga gcgatccgac cacgttggcg ggaaaatcct
     4261 taagattttt ccgccatgct tgcaaggggg cggttcgctt cggcggttcg gcgagcttgc
     4321 gagccgttca aagcccccgt tatctaacaa ggggggcaca aaaaacaaga aacactatac
     4381 actaacaagc ggaaaacgct gtatttataa ggcaaaaccc catttttaca gtagtgatat
     4441 aaattgtacc tatatcacta tgaaaattga atagtaaata tgagaaagac agttgatttc
     4501 aaaaaataag aaattgattg tcgttttctt ttgcagaaat ttaagtgcag gcaattatgg
     4561 tagttgtcgt aattgctgtc agctcagtgt ttatcactga tggtgtgaaa gatttacggt
     4621 tggagatgtt caataagatt attggtgctt aattattttt attgtagtgc tgatatgaga
     4681 atgttataat tggggtataa aacatacaaa taaatttgct gaaaaatgag gtatcaaaat
     4741 atggaaaatg aattactgga aaacttagga agacttcaca caactgaact tggcgttatt
     4801 agaattaaaa agaacttatc tttgaatgta gaaaatgtaa ttgaatggtg caaagaaaaa
     4861 attagccttt ctaatgcgga aatcacaaga aaaggaaaaa actggtatat aacgattgat
     4921 aattgtatta taacggtaaa tgcgtacagt tataccgtta tcactgctca caaggtaaaa
     4981 tgataaattt ttctataaag gaattaaacg catttgcagt tattgggcag gaagtagaac
     5041 tgactaatta tcaaaaaaag aatattcaga ttagtacaca gttttggaga aagtttaaca
     5101 gtagtttgaa aaaagcgtat ctttcacaat caggaaattg ggtaaaatat gcttttatgg
     5161 aaagaagaaa tggaaaactt tattatttct gttctattcc ccaaagaacc attaccccag
     5221 agggctttct gtataaagaa ataccgtctt ataaatattt ggttgtggag catattggtg
     5281 ctatggataa aatatatgaa acttatggca agatttatga agaaattatt cctagtacat
     5341 cgtatattcc tataaaagat attatcctac actttgaaaa atacgattat cgttttcatt
     5401 ggaatagtga taattcagtt attgaaattt ggatacctat tcaagattag aagcaatttt
     5461 atatccatac acacaaagca gttagtctta ggattgactg ctttttctat acagattttt
     5521 aatgacaggc aattatcaat tatggtagtt gccttttttg attggaggaa ttgaagaatg
     5581 aatgataaaa actttattga agaactaaga caaaagcgtg aggaatacgg agtaacacaa
     5641 acaaggcttg ctgttgcctg tggtatcagc cgtgaatatt acaaccgcat tgaaaaagga
     5701 aaacagccat tgaatgacga attaagagaa atcatagaaa aacagattga gcgtttcaat
     5761 ccgcaagaac cactattctt attgattgat tactttcgtg tccgctttcc tactacggac
     5821 gcattggcga ttatccgtga tgtattacaa ctgaaatcta attacatgct ttatgaagat
     5881 tatggaaaat acggatatga aagcaaatat gtacttggcg acatcaatat catgtgttcc
     5941 atgcaggagc atttaggtgt tttattggaa ctgaaaggca aaggctgtcg gcaaatggaa
     6001 tgttatctgc tggcacagga acgctcatgg tatgacttca tgctggattg tatgacggct
     6061 ggtggtgtga tgaaacggct tgatttggct atcaatgata aagcaggaat attggatatt
     6121 ccaaagttaa aggaaaaata caaggctggc gaatgtatgt cctacttccg tatgcagaaa
     6181 gattacagcg gtacagaaaa atgcggtagc gatttaccaa agaatacagg agaaacctta
     6241 tatctcggtt caacaagtag cgaattatat atgtgtgctt atcagaaaaa ttatgagca
//
LOCUS       DACBZN010000333         5561 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.137, whole genome shotgun sequence.
ACCESSION   DACBZN010000333 DACBZN010000000
VERSION     DACBZN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.137"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            103..363
                     /locus_tag="IVD64_003104"
                     /pseudo
     CDS             103..363
                     /locus_tag="IVD64_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            526..1191
                     /locus_tag="IVD64_003105"
                     /pseudo
     CDS             526..1191
                     /locus_tag="IVD64_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1227..1472
                     /locus_tag="IVD64_003106"
     CDS             1227..1472
                     /locus_tag="IVD64_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6150261.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1575..2444
                     /locus_tag="IVD64_003107"
     CDS             1575..2444
                     /locus_tag="IVD64_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6150262.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2425..3159
                     /locus_tag="IVD64_003108"
     CDS             2425..3159
                     /locus_tag="IVD64_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6150263.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3192..4107
                     /gene="ant(6)"
                     /locus_tag="IVD64_003109"
                     /pseudo
     CDS             3192..4107
                     /gene="ant(6)"
                     /locus_tag="IVD64_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            4104..4646
                     /gene="sat4"
                     /locus_tag="IVD64_003110"
     CDS             4104..4646
                     /gene="sat4"
                     /locus_tag="IVD64_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP6150264.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4739..5533
                     /locus_tag="IVD64_003111"
                     /note="aph(3')-IIIa"
     CDS             4739..5533
                     /locus_tag="IVD64_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6150265.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         1921 a          829 c         1220 g         1591 t
ORIGIN      
        1 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
       61 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
      121 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
      181 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
      241 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
      301 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
      361 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
      421 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
      481 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
      541 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
      601 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
      661 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
      721 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
      781 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
      841 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
      901 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
      961 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     1021 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     1081 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     1141 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     1201 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     1261 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     1321 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     1381 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     1441 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     1501 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     1561 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     1621 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     1681 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     1741 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     1801 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     1861 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     1921 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     1981 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     2041 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     2101 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     2161 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     2221 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     2281 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     2341 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     2401 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     2461 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     2521 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     2581 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     2641 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     2701 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     2761 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     2821 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     2881 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     2941 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     3001 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     3061 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     3121 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     3181 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     3241 aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt aatataccta
     3301 aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa cctagaaccg
     3361 tttatatcta atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg
     3421 gaggatatgg aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat
     3481 gattacaata aaattgatct taccttattg cccttggaag agttagataa ttacctaaag
     3541 ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt
     3601 ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc
     3661 aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta
     3721 tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg
     3781 aaggtcggca tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa
     3841 aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat
     3901 gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag
     3961 gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc
     4021 agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct
     4081 gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata
     4141 tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg
     4201 agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg
     4261 aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga
     4321 taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt
     4381 ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca
     4441 taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac
     4501 taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt
     4561 tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag
     4621 ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt
     4681 tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat
     4741 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
     4801 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
     4861 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
     4921 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg
     4981 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
     5041 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
     5101 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgattagc
     5161 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
     5221 agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
     5281 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
     5341 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
     5401 tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt
     5461 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
     5521 tgaattgttt tagtacctag atttagatgt ctaaaaagct t
//
LOCUS       DACBZN010000334         4489 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.154, whole genome shotgun sequence.
ACCESSION   DACBZN010000334 DACBZN010000000
VERSION     DACBZN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.154"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            42..989
                     /locus_tag="IVD64_003112"
     CDS             42..989
                     /locus_tag="IVD64_003112"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6150266.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1009..1536
                     /locus_tag="IVD64_003113"
     CDS             1009..1536
                     /locus_tag="IVD64_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6150267.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1599..2252)
                     /locus_tag="IVD64_003114"
     CDS             complement(1599..2252)
                     /locus_tag="IVD64_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6150268.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2384..3226
                     /locus_tag="IVD64_003115"
     CDS             2384..3226
                     /locus_tag="IVD64_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6150269.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3384..4280
                     /locus_tag="IVD64_003116"
     CDS             3384..4280
                     /locus_tag="IVD64_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6150270.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            4283..>4489
                     /locus_tag="IVD64_003117"
     CDS             4283..>4489
                     /locus_tag="IVD64_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6150271.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNK"
BASE COUNT         1615 a          807 c          896 g         1171 t
ORIGIN      
        1 aaaactatta gacaaatgat attttacgag gaggaaaaag gatggaacaa gcgtatcttg
       61 acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca ggcacaggca
      121 cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt cctttgttaa
      181 caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt ttaaaaggag
      241 atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa tgggcatttg
      301 aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt ggccatagat
      361 gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt ttttgcgaga
      421 agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac atctacggct
      481 atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc aaacaagtga
      541 tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca tggaatccgg
      601 aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt tatgttcaag
      661 agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta ggcgttccgt
      721 tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca ggtttagcgg
      781 ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat atcgaacaaa
      841 tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg aatcaggaaa
      901 agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat gacccacatc
      961 cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat gttagctgca
     1021 atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc ttggcgtttg
     1081 ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt gatgggacga
     1141 aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat tgttttgacg
     1201 agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga agacgtgctt
     1261 gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc tgtttatcaa
     1321 gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt attcgatgga
     1381 gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac tagtcaagga
     1441 gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg taatgagaat
     1501 cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata gactagctgt
     1561 tatttagagc tgtcccatcc atagctttat tttttttatt aagtaaagaa taaaacagag
     1621 aagtacataa aacctgcacc taacataaca aacaaatgcc aaacaacatg catatagcgg
     1681 atagaattca agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc tgctaccatc
     1741 aaaataacac cagtcattcc aagagagtga taaagaggaa cagcagccac gacacagagc
     1801 caccccataa aaatatagat gatcgtagat acttttgata ctctctcccg tttatgcaac
     1861 gtcaacgctt tatatataat gccaagaatc gcaaacaacc agattaatac aaataaagtc
     1921 caacctagcc atcctcggat actcagcaaa caaaaaggag tataacttcc tgcaatcaaa
     1981 agaaaaatcg aactatgatc gaatacttgg aacacctttt ttgcttttgt aaaaatcaat
     2041 gaatgaaaca aagtagacgt taaaaataaa agaatcatca tcgatccata gatagcataa
     2101 gagacaatat ggagggagga atgcaagtgg gcacctttta ctaaaagaac cactagtccc
     2161 gcgatactca atccagtccc aattccatgg gtgactgcat taaacacttc atttaatatt
     2221 tcatacgttc gattaaattt cgttttttcc atgaaaacgg ctcctttact aggtgtaatt
     2281 aatgaaaaat cattatttgt ctgttatact aaaaatagaa attgtcttac atgtagttta
     2341 acatgaataa ggcatcttgt taaagaaaga gagagagaaa ataatgacaa atgtaaaaat
     2401 cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc gatgctttag gcattcgtgt
     2461 catgccgcta tccatcatga tcgacggaac agtatatccc gatgatgacc aactagacgg
     2521 cgaaagattt atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa gccaaccacc
     2581 aattggagaa ttcatctcga tgtatgatga gctaggagaa gacggcagtg aagtgatttc
     2641 gatccatatg acaaaaggct tgagtggaac agtagaagca gcaagacaag ccagtaatct
     2701 aacaaaaacg aaagtaacgg ttatcgacag tgataccact gaccaaggat tatcttttca
     2761 ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa tccttgaaga
     2821 agtcaaaaaa gtacaagaca atacaaaatt atacatcgga atatccacac tagataactt
     2881 ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg tctagtgttt tgaacatacg
     2941 cgtggtgatg aatttcgccc attctgaatt aatccctgta actaaggggc gaggaaagaa
     3001 aacgttcgat aaatggttta ataatttgaa aaatgaactg aaagaattgc caaatgtgcg
     3061 acaaatcggt atttcacatg cggatgcaaa agaattggcg caagaattca aagagggttt
     3121 gcaagaatta ttcccggaca tgcatatccc tgtactgcat acgaatccgg ttatcgctac
     3181 gcatactggt aaagacgcgt ttgcaatcat gtattatgta gattagtcga aaagaggata
     3241 agccctcagc ctgaaaggct tgcagcttat tctctttttc tttataaaat taggaaaata
     3301 attgttttct tttttcataa agactacctt tttgataaaa tttttgataa cattagaacc
     3361 aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg acatttgatc ctcgttttgt
     3421 tcccattatt aattgcgatc atcatctatg caggtttagc tgcttctata ccgaaagctg
     3481 gcccactaat gaaaccagta actactgtga gagaagatcc aaataaaaaa gaaaaacttc
     3541 actacatagc tatcggagat tcgctgactg aaggagtagg agatcaaacc aagcaaggag
     3601 gattcgttcc ccttgtcgca aatgatctaa aagaccgtta taatttgacc gcggttgaaa
     3661 cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat cttaaagcga gtaaaaacaa
     3721 aagaagaaat tcgaaacaat atcaaaacag ctgattttat cacactgact gttggcggta
     3781 atgatttgat gaaagttatc caaaataatt tattcgggtt aacggtcaat tcctttaaaa
     3841 agccactgaa aaactatcag gaaaatgtca cagaactatt ggaagaattt cgatccctca
     3901 ataaaaccgc acctatttac attttaggca tctacaatcc attttatttg aactttcctg
     3961 agatcactga catgcagaca attgttaaca attggaacaa aggaacagaa acggtgacaa
     4021 aaaaaatgcc gaactgctac tttatcccaa tcaatgattt gctttatcaa ggattgaatc
     4081 aggaagtagg tgtcacagaa gaaggaacag atcaaacaac tagcacgaca gaaaatctaa
     4141 atggcatcaa aaataatgtg ctctatgaag aagatcattt ccatccaaat aatttaggtt
     4201 atcaattgat ggcaaatgca gtgagagatc agctgatcga gacacaaaac caatggttag
     4261 agaaggagga tgatcaataa acatgaaaac aagatctgac gtatcttcaa acaaaacaaa
     4321 gaaaacaaat tactggaaat tcgccttcct tatccttctt ggggtagtac ttggttcgac
     4381 cgtattttta ggcagccgta tttttgctaa tcgggaacca gaactcccag aaataccagc
     4441 tcttacggaa agacaaggag atcctgtctt aacgatcaat tcaaataaa
//
LOCUS       DACBZN010000335         3379 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.184, whole genome shotgun sequence.
ACCESSION   DACBZN010000335 DACBZN010000000
VERSION     DACBZN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.184"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..952
                     /locus_tag="IVD64_003118"
     CDS             <1..952
                     /locus_tag="IVD64_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6150272.1"
                     /translation="WRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDK
                     PTDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTE
                     GNPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFL
                     DKQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKK
                     IEQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPI
                     VLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1210..2712
                     /locus_tag="IVD64_003119"
     CDS             1210..2712
                     /locus_tag="IVD64_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6150273.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2779..3306
                     /locus_tag="IVD64_003120"
     CDS             2779..3306
                     /locus_tag="IVD64_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6150274.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1142 a          591 c          752 g          894 t
ORIGIN      
        1 atggcgtacc attgatggga tggatctctt attccgtcag cgaaaaatca gctatttatt
       61 gacaacttgg caaggaaaaa acaccgcagt catcgaatct gcacaagcat tggggttaga
      121 tctacttgat aaaccaacgg atcaaactat ccgacttgct tccagttatg ccttaggaga
      181 acttattaaa gatgcactgc ttcaagaagt agaacaaatt attgtcactt taggaggaag
      241 cgggtgtagc gatggtggtc taggattact ccaatccttg ggtgcttctt tagaagggta
      301 cacagaagga aatcctttat tatctactaa aaaacttcat tttgatcgaa gaaacgttct
      361 tgtttctaaa gtaaaattga ttatcgctgc agatgttacc aatccatata caggtcaaca
      421 aggagctgct tggcaatttg gaagacaaaa aggaggaata gatacgacgc tagctttctt
      481 agataaacaa gcaggaaaga ttgctcagca aataaaagat ttctgggagg tggatttaga
      541 tgatattcca ggaagtggag cagcaggcgg tattggtgga gccctttttt tgttgggagc
      601 gaagatggtt tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga
      661 acaagcagat gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa
      721 agttccatac ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg
      781 cggaagtaga gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat
      841 tcatttgggt ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac
      901 aaaaatctta ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa
      961 atcattttct ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt
     1021 gaaaaattaa gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag
     1081 cagtgtgcag attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta
     1141 tccaaccctt gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg
     1201 tgatggaaaa tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc
     1261 acattattat ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc
     1321 ggacgaaacg gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat
     1381 caagggcaag taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa
     1441 gaacgtttaa cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa
     1501 agagagctcc aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta
     1561 tcggggggag agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc
     1621 ccgttaatcg atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct
     1681 tatttgaaaa agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat
     1741 gaagtagtgg accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat
     1801 ttctctatct atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa
     1861 aaattgaaaa aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct
     1921 cgttcccgag aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga
     1981 cgagtgaata aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca
     2041 atcgtgaatc ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa
     2101 tatatcgatt ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta
     2161 gaagatcttc aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag
     2221 cctcatcagc gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat
     2281 tatcttctgg gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg
     2341 agcattagtt atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca
     2401 gagaaaaacc aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa
     2461 agagatgttt ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa
     2521 ttggctaaat ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat
     2581 ttggatgtct tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg
     2641 ttactagtgg aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt
     2701 gagagaatct aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc
     2761 aacgaaggag agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca
     2821 caaggattag tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca
     2881 cagaaactag cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc
     2941 cctcttcaac gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg
     3001 accgttgatg aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa
     3061 gatgagaact ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca
     3121 gtcttagatg tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa
     3181 aatgtctatc tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat
     3241 ccgatgccta atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat
     3301 gagtaattat gtttttgtgt tatttaaata aaatatttca aggcactgct tttgcttagc
     3361 agtgcctttt ttaggttat
//
LOCUS       DACBZN010000336         3047 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.192, whole genome shotgun sequence.
ACCESSION   DACBZN010000336 DACBZN010000000
VERSION     DACBZN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.192"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            121..1032
                     /locus_tag="IVD64_003121"
     CDS             121..1032
                     /locus_tag="IVD64_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAP6150275.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1185..1670
                     /gene="vanZ"
                     /locus_tag="IVD64_003122"
     CDS             1185..1670
                     /gene="vanZ"
                     /locus_tag="IVD64_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6150276.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1957..2286)
                     /locus_tag="IVD64_003123"
     CDS             complement(1957..2286)
                     /locus_tag="IVD64_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6150277.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2591..2920
                     /locus_tag="IVD64_003124"
     CDS             2591..2920
                     /locus_tag="IVD64_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6150278.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2910..>3047
                     /locus_tag="IVD64_003125"
     CDS             2910..>3047
                     /locus_tag="IVD64_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6150279.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KT"
BASE COUNT         1091 a          434 c          537 g          985 t
ORIGIN      
        1 ataattgact gtaatttacg gggcaaaacg gcacaatctc aaacgagatt gtgccgttta
       61 aggggaagat tctagaaata tttcatactt ccaactatat agttaaggag gagactgaaa
      121 atgaagaagt tgtttttttt attgttattg ttattcttaa tatacttagg ttatgactac
      181 gttaatgaag cactgttttc tcaggaaaaa gtcgaatttc aaaattatga tcaaaatccc
      241 aaagaacatt tagaaaatag tgggacttct gaaaataccc aagagaaaac aattacagaa
      301 gaacaggttt atcaaggaaa tctgctatta atcaatagta aatatcctgt tcgccaagaa
      361 agtgtgaagt cagatatcgt gaatttatct aaacatgacg aattaataaa tggatacggg
      421 ttgcttgata gtaatattta tatgtcaaaa gaaatagcac aaaaattttc agagatggtc
      481 aatgatgctg taaagggtgg cgttagtcat tttattatta atagtggcta tcgagacttt
      541 gatgagcaaa gtgtgcttta ccaagaaatg ggggctgagt atgccttacc agcaggttat
      601 agtgagcata attcaggttt atcactagat gtaggatcaa gcttgacgaa aatggaacga
      661 gcccctgaag gaaagtggat agaagaaaat gcttggaaat acgggttcat tttacgttat
      721 ccagaggaca aaacagagtt aacaggaatt caatatgaac catggcatat tcgctatgtt
      781 ggtttaccac atagtgcgat tatgaaagaa aagaatttcg ttctcgagga atatatggat
      841 tacctaaaag aagaaaaaac catttctgtt agtgtaaatg gggaaaaata tgagatcttt
      901 tattatcctg ttactaaaaa taccaccatt catgtgccga ctaatcttcg ttatgagata
      961 tcaggaaaca atatagacgg tgtaattgtg acagtgtttc ccggatcaac acatactaat
     1021 tcaaggaggt aaggatggcg gaatgaaacc aacgaaatta atgaacagca ttattgtact
     1081 agcacttttg gggtaacgtt agctttttaa tttaaaaccc acgttaacta ggacattgct
     1141 atactaatga tacaacttaa acaaaagaat tagaggaaat tatattggga aaaatattat
     1201 ctagaggatt gctagcttta tatttagtga cactaatctg gttagtgtta ttcaaattac
     1261 aatacaatat tttatcagta tttaattatc atcaaagaag tcttaacttg actccattta
     1321 ctgctactgg gaatttcaga gagatgatag ataatgttat aatctttatt ccatttggct
     1381 tgcttttgaa tgtcaatttt aaagaaatcg gatttttacc taagtttgct tttgtactgg
     1441 ttttaagtct tacttttgaa ataattcaat ttatcttcgc tattggagcg acagacataa
     1501 cagatgtaat tacaaatact gttggaggct ttcttggact gaaattatat ggtttaagca
     1561 ataagcatat gaatcaaaaa aaattagaca gagttattat ttttgtaggt atacttttgc
     1621 tcgtattatt gctcgtttac cgtacccatt taagaataaa ttacgtgtaa gatgtctaaa
     1681 tcaagcaatc tgatctttca tacacataaa gatattgaat gaattggatt agatggaaaa
     1741 cgggatgtgg ggaaactcgc ccgtaggtgt gaagtgaggg gaaaaccggt gataaagtaa
     1801 aaagcttacc taacactata gtaacaaaga aagcccaatt atcaatttta gtgctgagga
     1861 attggtctct ttaataaatt tccttaacgt tgtaaatccg cattttcctg acggtacccc
     1921 caaactaaga tttcatagat agattttctg ataactttaa aaaacacgaa tagcggaaag
     1981 gatgtcttca ttcgctaaag agtaataaac tacttttcca tcttgacgtg attttgccat
     2041 tccatgtttt tttaaaaaac gtaaatggtg tgacgttgta gcgacactag cagtaagtat
     2101 ttctgctaaa tcacagacgc acatttcctt ataggtttct aaagcataga aaattttaat
     2161 tctagaaaaa tcgctgaaac atttccctaa cacaagtaag tttgaaaaat ctttttgatc
     2221 caatttattt tttattccat ttactttttc tttatgtacg acagtcactt ggcaaacttt
     2281 tttcattcat ctcacctcac attcaaacga ttatttgaat gaagtatacc tatttgatca
     2341 ctcttttgtc aatctacctt aattccaata cactattata agcaatttca tgtattgaag
     2401 taaacttact tgcaagtgcg tttttgattc caagttaaaa acatattatt attctacata
     2461 atttcctaat caaataaaac tcctttgaaa attgttctaa aggagtttta ttattttgta
     2521 tttctactaa aaatagcgta caatgtaatt actaagtaat tacgaaatgt aaaataatgg
     2581 aggaaataat atgattacaa aaacaagaaa acaaggaaat tctattatgt taacggtccc
     2641 taaagaattc gatgtgccca atggtgttga agtagaagct aagcttgtag agaatggtat
     2701 cctttatgag tttgtagagc ctaaaaaaga attttttgat tttagtgaag atattttggc
     2761 tgatattctt tcagaaggtt atagcaaaca agagatctta aaagaattta gaaatagaaa
     2821 aaatgcgtta acttctgcat ttaaatctat tgctgaagat actgttgcaa attctaaacc
     2881 gatgacaaaa gaggagttgg cagcagaaat tggcttatga gattctacct tctaaacacg
     2941 taataaagta tctaaaaaaa ttaaaagaga aaacattaaa agaacaattt ttaacgataa
     3001 tttatgatga gatagcagtt cgtcctcatt cgggagaaca aaaaacg
//
LOCUS       DACBZN010000337         2775 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.198, whole genome shotgun sequence.
ACCESSION   DACBZN010000337 DACBZN010000000
VERSION     DACBZN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.198"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..468
                     /locus_tag="IVD64_003126"
     CDS             <1..468
                     /locus_tag="IVD64_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6150280.1"
                     /translation="FSVEQVITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKP
                     QKSDYQPKQLESDHTVDTETMDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKE
                     YVFEELVNPIFTRKDNQVIVNVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            713..829
                     /locus_tag="IVD64_003127"
     CDS             713..829
                     /locus_tag="IVD64_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6150281.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            845..2764
                     /gene="tet(M)"
                     /locus_tag="IVD64_003128"
     CDS             845..2764
                     /gene="tet(M)"
                     /locus_tag="IVD64_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6150282.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          971 a          426 c          536 g          842 t
ORIGIN      
        1 ttttctgttg aacaagttat taccgaagac aaagacaagg aaactatatc gtctagcttt
       61 catgtggttg tccatataga tgaatcagat aatatggtta ttatcaaaaa tccgacaatg
      121 agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc tagaaagtga ccatacagta
      181 gatacagaaa cgatggacga aatcactagc tttttagaaa cgttcttcca gctctatcct
      241 accgccacag aaaaagaatt gacttactat gtaagtaatc atgtgttacc tatgattaac
      301 aaggaatatg ttttcgagga attggtgaat cctatcttta ccagaaaaga taaccaagta
      361 atcgtgaatg tcgctgtgaa atatttagat caagaaacaa aggcaaccca aatctcgcaa
      421 tttgagttga tattagaaaa acaagataat tggaagattg taaaataaca aatattggta
      481 catgattaca gatactttgt aatcatgtac tctttttgat aaaaaattgg agattccttt
      541 acaaatatgc tcttacgtgc tattatttaa gtgactattt aaaaggagtt aataaatatg
      601 cggcaaggta ttcttaaata aactgtcaat ttgatagcgg gaacaaataa ttagatgtcc
      661 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
      721 aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata aaaatcccag
      781 tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa
      841 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt
      901 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
      961 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
     1021 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
     1081 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
     1141 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
     1201 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
     1261 ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac agaaggtaga
     1321 actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg atacggtaat
     1381 agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattgga
     1441 actcgaacaa gaggaaagca taagatttca gaattgttct ctgttccctc tttatcatgg
     1501 aagtgcaaaa agtaatatag ggattgataa ccttatagaa gttattacta ataaatttta
     1561 ttcatcaaca catcgaggtc cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac
     1621 aaaaaaaaga caacgtcttg catatatacg cctttatagt ggagtactac atttacgaga
     1681 ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca gaaatgtata cttcaataaa
     1741 tggtgaatta tgtaagattg atagagctta ttctggagaa attgttattt tgcaaaatga
     1801 gtttttgaag ttaaatagtg ttcttggaga tacaaaacta ttgccacaga gaaaaaagat
     1861 tgaaaatccg caccctctac tacaaacaac tgttgaaccg agtaaacctg aacagagaga
     1921 aatgttgctt gatgcccttt tggaaatctc agatagtgat ccgcttctac gatattacgt
     1981 ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa tggaagtgat
     2041 tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc ctacagtcat
     2101 ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag tgccgccaaa
     2161 tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa gtggaatgca
     2221 gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg cagttatgga
     2281 agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg attgtaaaat
     2341 ctgttttaag tacggtttat actatagccc tgttagtact ccagcagatt ttcggatgct
     2401 tactcctatt gtactggagc aagcctttag aaaagctgga acagaattgt tagagccata
     2461 tcttagtttt aaagtttatg caccacagga atatctttca cgggcatata acgatgctcc
     2521 caaatattgt gcaaatatcg taaatactca actgaaaaat aatgaggtca ttattattgg
     2581 agaaattcct gctcgatgta ttcaagatta tcgcaatgat ttaacttttt ttacaaatgg
     2641 gcttagtgtt tgtttagcag agctaaaagg atatcaggtt accactggcg aacctgtttg
     2701 ccagacccgt cgtctaaata gtcggataga taaagtaaga tatatgttca ataaaataac
     2761 ttagtgcgtt ttatg
//
LOCUS       DACBZN010000338         2585 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.201, whole genome shotgun sequence.
ACCESSION   DACBZN010000338 DACBZN010000000
VERSION     DACBZN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.201"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            104..1072
                     /locus_tag="IVD64_003129"
     CDS             104..1072
                     /locus_tag="IVD64_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6150283.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1065..2096
                     /gene="vanA"
                     /locus_tag="IVD64_003130"
     CDS             1065..2096
                     /gene="vanA"
                     /locus_tag="IVD64_003130"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6150284.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2102..>2585
                     /locus_tag="IVD64_003131"
     CDS             2102..>2585
                     /locus_tag="IVD64_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP6150285.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     Q"
BASE COUNT          756 a          499 c          648 g          682 t
ORIGIN      
        1 gtaagaacga gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc
       61 ccgttttatt tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat
      121 tactgtttat ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt
      181 tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc
      241 tttcaatcaa tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc
      301 gctgaagaga gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga
      361 tacaactgct gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag
      421 cgttgccgat tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt
      481 gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga
      541 catgacagtt ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg
      601 aggatttgga tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt
      661 accgtttgat gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac
      721 ggatacgcac tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct
      781 tatcaatact gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa
      841 cgggaaactg ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc
      901 tgattgcacc caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa
      961 cgtgataatc acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga
     1021 aaaaaccatt aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag
     1081 ttgcaatact gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag
     1141 agatagccgc taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat
     1201 ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt
     1261 cagctgtact ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat
     1321 atgaaatcaa ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg
     1381 gatccataca aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa
     1441 gctcagcaat ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag
     1501 ctactcccgc cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct
     1561 atcctgtttt tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata
     1621 gcgcggacga attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa
     1681 ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt
     1741 tagctgttgg cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg
     1801 aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag
     1861 cagaggagcg aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta
     1921 gaggtctagc ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag
     1981 tcaatactct gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag
     2041 gtattgcact tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag
     2101 catggaaata ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa
     2161 atatgccact tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat
     2221 tgtagggaca tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca
     2281 agggtacgga ttgcttctat gggacggtta ccgtcctaat cgtgctgtaa actgttttat
     2341 gcaatgggct gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga
     2401 ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag
     2461 tgccattgat cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg
     2521 atttgatttt atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc
     2581 gcaaa
//
LOCUS       DACBZN010000339         2356 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.208, whole genome shotgun sequence.
ACCESSION   DACBZN010000339 DACBZN010000000
VERSION     DACBZN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.208"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            268..1746
                     /gene="msr"
                     /locus_tag="IVD64_003132"
     CDS             268..1746
                     /gene="msr"
                     /locus_tag="IVD64_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6150286.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1793..2239
                     /locus_tag="IVD64_003133"
     CDS             1793..2239
                     /locus_tag="IVD64_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6150287.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT          788 a          385 c          504 g          679 t
ORIGIN      
        1 gcatcgatga aattacgttt cgaacttttg aataacaact aaataaattc aaaagacatg
       61 tataaacaac aatttcatgg atggagtatg tccatttttt cgaacgacag tagaataggt
      121 gaaagctatg acagatttgg tgatttagtc atagttattt cgttcagagt aggaaaagat
      181 gtgatgcagt ttttttgcaa acagattttt ctgctctttt ttagtatcga taattgttgt
      241 tttacatgaa ataggagtgt aaaacatatg gaaaatttag cagtaaatat aacaaatcta
      301 caagtcagct ttggcaacca gctagaacta tctattgatt ctcttcgtgt ctatcagcaa
      361 gatcgtatag ggatcatagg agaaaatggt gtcggtaaat cgactttgct caaactaata
      421 gccggtgaac tttttcccga tcatgggaaa atccaaacag agatcacctt caactacctg
      481 cctcaattaa cctatctcgc tgaagcaaag gacctaaatt tggaattagc cagtcatttc
      541 cagttaagac tggaagaaac ttcagagcgg aaatggagcg gaggggaaga acgaaagatc
      601 gagttgatac gtcttctttc ttcttatgaa caagggatgc ttctagacga gccaacaacc
      661 catctagata gaaaaagtat tgatcgactg attgaagagc ttcgttatta ttatggtacg
      721 ctggtttttg ttagtcatga tcgctatttt ctagatgagc tagcatcgaa aatctgggaa
      781 gtaaaagacg gagaaatccg agagttttcg gggaattata gtgcctatct cactcaaaag
      841 gaattggaga aaaagactca gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga
      901 ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt
      961 aaaaagaaaa agcaacaaat cagaccggat cggttgtctt cctctaaaca aaaagacagt
     1021 gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga gaagactcca aaaaatagga
     1081 gaaacaacca agccgcagca gatgaaacaa atccgttttc cagtaccaaa atctcttgaa
     1141 ctccacagcc gctatcccat catgggacaa aatgtccaat tggaaagaag tgggagaaca
     1201 ttactggtaa atggtgattt tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa
     1261 aatggttcag gtaagacaac cttattagaa catatccgca aacaaggaga aggaatcctt
     1321 ctctctccga aagtaagctt tcaagtatat cagcaaaagg gttatcaaat gacatctgaa
     1381 gaatccatca ttcgttttgt catgagacaa acagagtttt cagaatcgct tgtccgtagt
     1441 ttgctgaatc acttagggtt tgctcaggaa actttgacga aaccgttatg tacattaagt
     1501 gggggagaag cgacccgtct gacgattgct ttgcttttta ctaagccaag taatgtgttg
     1561 ctgttagatg aaccgactaa ttttattgat atggcaacga tcgaagcttt agagaagcta
     1621 atgcaaatat atccgggaac gattttgttt acttctcatg attcttactt tgtcgagcgt
     1681 acggctgatg aagtttatga aataaaaggg cagaaaataa aaaaagtact tacgagaaat
     1741 ttttaaatgt gctacgatga ttttcgtgac aaatttaaag gagtggtaca gattgcctac
     1801 aaataaaaaa gaaggaataa tttttacaac aatgatgtgt tttttgatgg tcttggggat
     1861 gagcgcgtat aatttatggc tgcatcaaga tcttcattta tcaagtttgt tgattggtct
     1921 agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa
     1981 aaaaattgcc tttaaactac cagtaaatcc agaaagcaag gtccaaatga tcttaactat
     2041 ttcatgcttg atggttttag gaatggtgac atgtatgtct ttattcggtg tgatcatgga
     2101 acaaggaata cctgatcaat tattctctgc ttatatgaat gcttggaaaa tgaatgtgat
     2161 tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc
     2221 aattcaaagt aatgaatgat ggaacactgt tatttgaaaa aaggccttgg catgcttttt
     2281 gtcatagtct cttgaatgca gttctagaaa aatagtgatc cgaacgatag gttttggcct
     2341 gtcgttcgga tcgctt
//
LOCUS       DACBZN010000340         1453 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.236, whole genome shotgun sequence.
ACCESSION   DACBZN010000340 DACBZN010000000
VERSION     DACBZN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.236"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            <1..492
                     /locus_tag="IVD64_003134"
     CDS             <1..492
                     /locus_tag="IVD64_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAP6150288.1"
                     /translation="VKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCAVLGNSA
                     ALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETAKKIYKA
                     LGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELIDRLIVLA
                     LKG"
     gene            498..1106
                     /locus_tag="IVD64_003135"
     CDS             498..1106
                     /locus_tag="IVD64_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6150289.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          429 a          285 c          365 g          374 t
ORIGIN      
        1 gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat
       61 gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg
      121 ggaaacagtg ccgcgttagc tgttggcgag gtggaccaaa tcaggctgca gtacggaatc
      181 tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt
      241 cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat
      301 aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc
      361 attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt
      421 atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg
      481 ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg
      541 ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt
      601 atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag
      661 aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt cctaatcgtg
      721 ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt
      781 attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa
      841 gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg
      901 taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa
      961 tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt
     1021 ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata
     1081 gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc
     1141 taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt
     1201 agatagtatt gaagatgtaa ggcagagcga tattgcggtc attatctgcg tgcgctgcgg
     1261 caagatagcc tgataataag actgatcgca tagaggggtg gtatttcaca ccgcccattg
     1321 tcaacaggca gttcagcctc gttaaattca gcatgggtat cacttatgaa aattcatcta
     1381 cattggtgat aatagtaaat ccagtagggc gaaataattg actgtaattt acggggcaaa
     1441 acggcacaat ctc
//
LOCUS       DACBZN010000341         1289 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.245, whole genome shotgun sequence.
ACCESSION   DACBZN010000341 DACBZN010000000
VERSION     DACBZN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.245"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            574..1122
                     /gene="aac(6')"
                     /locus_tag="IVD64_003136"
     CDS             574..1122
                     /gene="aac(6')"
                     /locus_tag="IVD64_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6150290.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          455 a          188 c          257 g          389 t
ORIGIN      
        1 ctaaaattta tatcttgact tattaccatt aggctttctt cgttgaaacg ggttaaaatt
       61 ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga
      121 taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga
      181 acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac
      241 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
      301 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
      361 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
      421 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
      481 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
      541 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
      601 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
      661 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
      721 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
      781 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
      841 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
      901 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
      961 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
     1021 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
     1081 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
     1141 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt
     1201 gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga
     1261 aaaagtcgct tagtttcgag tagtaagaa
//
LOCUS       DACBZN010000342         1244 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.252, whole genome shotgun sequence.
ACCESSION   DACBZN010000342 DACBZN010000000
VERSION     DACBZN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.252"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            37..1191
                     /locus_tag="IVD64_003137"
     CDS             37..1191
                     /locus_tag="IVD64_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6150291.1"
                     /translation="MAIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          405 a          229 c          277 g          333 t
ORIGIN      
        1 ttgataatcc gaaatatata aaaacggtat ggggggttgg ctataaaatt gaaaaataaa
       61 aaaaacgact attccaaact agaacgaaaa ctttacatgt atatcgttgc aattgttgtg
      121 gtagcaattg tattcgtgtt gtatattcgt tcaatgatcc gagggaaact tggggattgg
      181 atcttaagta ttttggaaaa caaatatgac ttaaatcacc tggacgcgat gaaattatat
      241 caatattcca tacggaacaa tatagatatc tttatttatg tggcgattgt cattagtatt
      301 cttattctat gtcgcgtcat gctttcaaaa ttcgcaaaat actttgacga gataaatacc
      361 ggcattgatg tacttattca gaacgaagat aaacaaattg agctttctgc ggaaatggat
      421 gttatggaac aaaagctcaa cacattaaaa cggactctgg aaaagcgaga gcaggatgca
      481 aagctggccg aacaaagaaa aaatgacgtt gttatgtact tggcgcacga tattaaaacg
      541 ccccttacat ccattatcgg ttatttgagc ctgcttgacg aggctccaga catgccggta
      601 gatcaaaagg caaagtatgt gcatatcacg ttggacaaag cgtatcgact cgaacagcta
      661 atcgacgagt tttttgagat tacacggtat aacctacaaa cgataacgct aacaaaaacg
      721 cacatagacc tatactatat gctggtgcag atgaccgatg aattttatcc tcagctttcc
      781 gcacatggaa aacaggcggt tattcacgcc cccgaggatc tgaccgtgtc cggcgaccct
      841 gataaactcg cgagagtctt taacaacatt ttgaaaaacg ccgctgcata cagtgaggat
      901 aacagcatca ttgacattac cgcgggcctc tccggggatg tggtgtcaat cgaattcaag
      961 aacactggaa gcatcccaaa agataagcta gctgccatat ttgaaaagtt ctataggctg
     1021 gacaatgctc gttcttccga tacgggtggc gcgggacttg gattggcgat tgcaaaagaa
     1081 attattgttc agcatggagg gcagatttac gcggaaagca atgataacta tacgacgttt
     1141 agggtagagc ttccagcgat gccagacttg gttgataaaa ggaggtccta agagatgtat
     1201 ataattttgg ctctttgtca aatgatgttg gtaaagagaa atct
//
LOCUS       DACBZN010000343         1133 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.259, whole genome shotgun sequence.
ACCESSION   DACBZN010000343 DACBZN010000000
VERSION     DACBZN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.259"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            81..164
                     /locus_tag="IVD64_003138"
     CDS             81..164
                     /locus_tag="IVD64_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6150292.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            289..1026
                     /gene="erm(B)"
                     /locus_tag="IVD64_003139"
     CDS             289..1026
                     /gene="erm(B)"
                     /locus_tag="IVD64_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6150293.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1031..>1133
                     /locus_tag="IVD64_003140"
     CDS             1031..>1133
                     /locus_tag="IVD64_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HAP6150294.1"
                     /translation="MSRFFKFGKLHVTKGNGDKLLDILLTASKKLKRS"
BASE COUNT          422 a          188 c          182 g          341 t
ORIGIN      
        1 cctatagagg atgggcgact tctgcctaat tctgttaaat tagatgctaa aaatttgtaa
       61 ttaagaagga gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta
      121 ctgttttgaa acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt
      181 cctaccagtg actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt
      241 ttaacttctg tatttattta cagatgtaat aacttcagga gtgattacat gaacaaaaat
      301 ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa
      361 ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg
      421 acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc
      481 aacttatcgt cagaaaaatt aaaactgaac attcgtgtca ctttaattca ccaagatatt
      541 ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat
      601 ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg
      661 attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc
      721 ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct
      781 aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat
      841 aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa
      901 ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt
      961 accgttactt atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg
     1021 aaataattct atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg
     1081 agataaatta ttagatatac tactgacagc ttccaagaag ctaaagaggt ccc
//
LOCUS       DACBZN010000344          963 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain EF20PII
            SAMN15598751-rid10849513.guided.271, whole genome shotgun sequence.
ACCESSION   DACBZN010000344 DACBZN010000000
VERSION     DACBZN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15598751
            Sequence Read Archive: SRR12286378
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000866090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000866090.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2020 04:39:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 34 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15598751-rid10849513.guided.271"
                     /strain="EF20PII"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: San Diego, CA"
                     /collected_by="UCSD SBRG"
     gene            246..941
                     /gene="vanR"
                     /locus_tag="IVD64_003141"
     CDS             246..941
                     /gene="vanR"
                     /locus_tag="IVD64_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6150295.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
BASE COUNT          344 a          176 c          192 g          251 t
ORIGIN      
        1 tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata
       61 tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc
      121 cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc
      181 ttataaattc ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga
      241 taactatgag cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg
      301 aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat
      361 tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca
      421 caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc
      481 tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt
      541 atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc
      601 gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc
      661 ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc
      721 ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg
      781 agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca
      841 ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata
      901 taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa
      961 cta
//
